Works (65)

Updated: April 3rd, 2024 19:41

2024 article

The scholarship of honorifics

Davis, J. M., Angle, J. S., Ferl, R. J., Gilbert, R. A., Grunden, A. M., Rials, T. G., … Toews, M. D. (2024, March 3). CROP SCIENCE, Vol. 3.

By: J. Davis*, J. Angle*, R. Ferl*, R. Gilbert*, A. Grunden n, T. Rials*, S. Senseman*, G. Thompson*, M. Toews*

Sources: Web Of Science, ORCID
Added: March 25, 2024

2023 journal article

Biochemical characterization of a psychrophilic and halotolerant a-carbonic anhydrase from a deep-sea bacterium, Photobacterium profundum

AIMS MICROBIOLOGY, 9(3), 540–553.

By: V. Somalinga*, E. Foss* & A. Grunden n

author keywords: Photobacterium profundum; a-carbonic anhydrase; halotolerance; biomineralization; carbon capture
TL;DR: The first biochemical characterization of P. profundum α–CA (PprCA) is reported which revealed several catalytic properties that are atypical for this class of CA's and it is demonstrated that PprCA accelerates CO2 biomineralization to calcium carbonate under alkaline conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: July 19, 2023

2023 article

Nitrogen Fixation and Ammonium Assimilation Pathway Expression of Geobacter sulfurreducens Changes in Response to the Anode Potential in Microbial Electrochemical Cells

Ortiz-Medina, J. F., Poole, M. R., Grunden, A. M., & Call, D. F. (2023, March 28). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 3.

By: J. Ortiz-Medina n, M. Poole n, A. Grunden n & D. Call n

author keywords: electromicrobiology; Geobacter; nitrogen fixation
MeSH headings : Nitrogen Fixation; Ammonium Compounds / metabolism; Geobacter / metabolism; Electrodes; Nitrogenase / metabolism; Nitrogen / metabolism
TL;DR: The anode potential in microbial electrochemical technologies has a significant impact on nitrogen gas fixation rates, ammonium assimilation pathways, and expression of genes associated with nitrogen gasfixing in Geobacter sulfurreducens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: April 11, 2023

2023 article

Streptomyces spp. as biocatalyst sources in pulp and paper and textile industries: Biodegradation, bioconversion and valorization of waste

Cuebas-Irizarry, M. F., & Grunden, A. M. (2023, April 5). MICROBIAL BIOTECHNOLOGY, Vol. 4.

By: M. Cuebas-Irizarry n & A. Grunden n

TL;DR: This review discusses reports of Streptomyces‐based technologies for use in the textile and pulp‐milling industry and describes the challenges and recent advances aimed at achieving better biodegradation methods featuring these microbial catalysts. (via Semantic Scholar)
Sources: ORCID, Web Of Science
Added: April 6, 2023

2023 journal article

Survivability of Salmonella Typhimurium (ATCC 14208) and Listeria innocua (ATCC 51742) on lignocellulosic materials for paper packaging

HELIYON, 9(3).

By: J. Zwilling n, J. Whitham n, F. Zambrano n, A. Pifano n, A. Grunden n, H. Jameel n, R. Venditti n, R. Gonzalez n

author keywords: Lignocellulosic; Food safety; Paper; Survivability; Bacteria; Packaging
TL;DR: This research compared the growth and survivability of Salmonella Typhimurium and Listeria innocua on bleached and unbleached paper packaging materials to understand how bacteria proliferate and survive on lignocellulosic surfaces. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: May 9, 2023

2023 journal article

Trimming and Decontamination of Metagenomic Data can Significantly Impact Assembly and Binning Metrics, Phylogenomic and Functional Analysis

CURRENT BIOINFORMATICS, 18(5), 428–439.

By: J. Whitham n & A. Grunden n

author keywords: Trimming; decontamination; binning; assembly; metagenomics; MAG; QC; accessibility
Sources: Web Of Science, ORCID
Added: August 28, 2023

2022 article

Diversity and Structure of Bacterial Communities in Different Rhizocompartments (Rhizoplane, Rhizosphere, and Bulk) at Flag Leaf Emergence in Four Winter Wheat Varieties

Zervas, A., Ellegaard-Jensen, L., Hennessy, R. C., Bak, F., Guan, Y., Horn Herms, C., … Nicolaisen, M. H. (2022, April 13). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 4.

By: A. Zervas*, L. Ellegaard-Jensen*, R. Hennessy*, F. Bak*, Y. Guan*, C. Horn Herms*, K. Molina Zamudio*, D. Thybo Ganzhorn* ...

TL;DR: 16S amplicon sequencing data from 3 rhizocompartments of 4 wheat cultivars grown under controlled greenhouse conditions observed that rhizOCompartments and cultivar affect the community composition. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: May 2, 2022

2022 article

Microbial Diversity in Four Rhizocompartments (Bulk Soil, Rhizosphere, Rhizoplane, and Endosphere) of Four Winter Wheat Varieties at the Fully Emerged Flag Leaf Growth Stage

Ahmad, J., Zervas, A., Ellegaard-Jensen, L., Hennessy, R. C., Carbone, I., Cornish, V., … Nicolaisen, M. H. (2022, October 6). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 10.

TL;DR: Results of 16S rRNA amplicon sequencing from four rhizocompartments for four wheat cultivars grown under controlled conditions and sampled after flag leaf emergence demonstrate differences in microbial communities according to rhizcompartment. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, ORCID
Added: October 24, 2022

2022 article

Plasma-driven biocatalysis: In situ hydrogen peroxide production with an atmospheric pressure plasma jet increases the performance of OleT(JE) when compared to adding the same molar amount of hydrogen peroxide in bolus

Wapshott-Stehli, H. L., Myers, B. G., Quesada, M. J. H., Grunden, A., & Stapelmann, K. (2022, February 3). PLASMA PROCESSES AND POLYMERS, Vol. 2.

author keywords: atmospheric pressure plasma jet; biocatalysis; cytochrome P450 enzymes; hydrogen peroxide; plasma liquid chemistry
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science
Added: February 4, 2022

2021 journal article

A review of clothing microbiology: the history of clothing and the role of microbes in textiles

BIOLOGY LETTERS, 17(1).

By: D. Sanders n, A. Grunden n & R. Dunn n

author keywords: clothing microbiology; textiles; fibres; degradation; microbes in clothing; microbiome
MeSH headings : Clothing; Humans; Laundering; Microbiota; Textiles
TL;DR: The history of clothing and the evolution of textiles, what is and is not known about microbial persistence on and degradation of various fibres, and what opportunities for the industrial and environmental application of clothing microbiology exist for the future are discussed. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: February 15, 2021

2021 review

Bacterial valorization of pulp and paper industry process streams and waste

[Review of ]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 105(4), 1345–1363.

By: D. Brown n, J. Pawlak n & A. Grunden n

author keywords: Lignocellulose; Pulp and paper; Bacteria; Lignin; Cellulose; Hemicellulose
MeSH headings : Bacteria / metabolism; Biomass; Cellulose / metabolism; Fermentation; Industrial Waste / analysis; Lignin / metabolism; Paper
TL;DR: The composition of pulp and paper streams, bacterial isolates from process streams that can be used for lignocellulose biotransformations, and technological approaches for improving valorization efforts are discussed. (via Semantic Scholar)
Sources: ORCID, Web Of Science
Added: January 23, 2021

2021 journal article

In situ H2O2 generation methods in the context of enzyme biocatalysis

ENZYME AND MICROBIAL TECHNOLOGY, 145.

By: H. Wapshott-Stehli n & A. Grunden n

author keywords: Hydrogen peroxide; Peroxidases; Unspecific peroxygenases; Biocatalysis
MeSH headings : Biocatalysis; Heme; Hydrogen; Hydrogen Peroxide
TL;DR: Generation of hydrogen peroxide in situ for biocatalysis using enzymes, bioelectrocatalyis, photocatalysis, and cold temperature plasmas are addressed and particular emphasis is given to reaction processes that support high total turnover numbers (TTNs) of the hydrogenperoxide-requiring enzymes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: April 19, 2021

2021 review

Leveraging Pseudomonas Stress Response Mechanisms for Industrial Applications

[Review of ]. FRONTIERS IN MICROBIOLOGY, 12.

By: K. Craig*, B. Johnson* & A. Grunden n

author keywords: stress; Pseudomonas; formulation; heat; desiccation; cold; biofilm; chaperone
TL;DR: Mechanisms of survival are grouped by the type of inducing stress with a focus on adaptations such as synthesis of protective substances, biofilm formation, entering a non-culturable state, enlisting chaperones, transcription and translation regulation, and altering membrane composition. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: June 10, 2021

2021 journal article

Plasma agriculture: Review from the perspective of the plant and its ecosystem

Plasma Processes and Polymers.

By: P. Ranieri n, N. Sponsel n, J. Kizer n, M. Rojas‐Pierce n, R. Hernández n, L. Gatiboni n, A. Grunden n, K. Stapelmann n

author keywords: plant fertilizer; nonthermal plasma; plant development; seed germination; soil microbiome
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: ORCID
Added: October 1, 2020

2021 journal article

Structure, Function, and Thermal Adaptation of the Biotin Carboxylase Domain Dimer from Hydrogenobacter thermophilus 2-Oxoglutarate Carboxylase

BIOCHEMISTRY, 60(4), 324–345.

By: G. Buhrman n, P. Enriquez n, L. Dillard n, H. Baer n, V. Truong n, A. Grunden n, R. Rose n

MeSH headings : Bacteria / enzymology; Bacterial Proteins / chemistry; Carbon-Nitrogen Ligases / chemistry; Crystallography, X-Ray; Enzyme Stability; Hot Temperature; Protein Domains; Structure-Activity Relationship
TL;DR: Structural data reveal that the OGC BC dimer comprises a "wet" dimerization interface that is dominated by hydrophilic interactions and structural water molecules common to all BC domains and likely facilitates the conformational changes associated with the catalytic cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID
Added: January 20, 2021

2021 journal article

Temperature and solvent exposure response of three fatty acid peroxygenase enzymes for application in industrial enzyme processes

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 571, 60–65.

By: H. Wapshott-Stehli n & A. Grunden n

author keywords: Hydrogen peroxide; Cytochrome P450 (CYP450); Peroxygenases; Biocatalysis; OleT; CYP152K6
MeSH headings : Acetone / chemistry; Acetone / metabolism; Bacillus / enzymology; Dimethylformamide / chemistry; Dimethylformamide / metabolism; Fatty Acids / metabolism; Mixed Function Oxygenases / metabolism; Solvents / chemistry; Solvents / metabolism; Temperature
TL;DR: A fatty acid peroxygenase from Bacillus methanolicus (CYP152K6) was shown here to have a higher optimal reaction temperature than OleT and showed great stability in 3% acetone and dimethylformamide. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science
Added: July 23, 2021

2020 journal article

Bacterial exposure leads to variable mortality but not a measurable increase in surface antimicrobials across ant species

PEERJ, 8.

By: O. Halawani n, R. Dunn n, A. Grunden n & A. Smith n

author keywords: Entomopathogen; Metapleural gland; Social immunity; Bacterial exposure; Social insects; Sociobiology; Antimicrobial
TL;DR: The results demonstrate the susceptibility to bacteria is varied across ant species, and variation may correlate with an ant species’ use of surface antimicrobials, as significant mortality effects in species which also were producing antimicrobial compounds are found. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID
Added: January 11, 2021

2020 journal article

Thermostable endoglucanase gene derived by amplification from the genomic DNA of a cellulose-enriched mixed culture from mudspring water of Mt. Makiling, Laguna, Philippines

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 36(3).

By: R. Tambalo*, A. Raymundo* & A. Grunden n

author keywords: Endoglucanase; Saccharolobus; Mt; Makiling mudspring; Lignocellulose hydrolysis; Thermophiles
MeSH headings : Amino Acid Sequence; Archaea / enzymology; Archaea / genetics; Bacteria / enzymology; Bacteria / genetics; Base Sequence; Cellulase / genetics; Cellulase / metabolism; Cellulose / metabolism; Cloning, Molecular; DNA; Enzyme Stability; Escherichia coli / genetics; Genomics; Hydrogen-Ion Concentration; Molecular Weight; Philippines; Recombinant Proteins; Sequence Alignment; Sulfolobales / enzymology; Sulfolobales / genetics; Temperature; Water / metabolism; Water Microbiology
TL;DR: The thermo-acid stable endoglucanase gene can be used in a supplementary hydrolysis step to further hydrolyze the lignocellulosic materials that were previously treated under high temperature-dilute acid conditions, thereby enhancing the release of more glucose sugars for bioethanol production. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: April 6, 2020

2019 journal article

Nitrogen Gas Fixation and Conversion to Ammonium Using Microbial Electrolysis Cells

ACS Sustainable Chemistry & Engineering, 7(3), 3511–3519.

By: J. Ortiz-Medina n, A. Grunden n, M. Hyman n & D. Call n

author keywords: Nitrogen fixation; Ammonium production; Haber-Bosch; Microbial electrochemical technology; Geobacter
TL;DR: This study explored the possibility of converting N2 into NH4+ using anaerobic, single-chamber microbial electrolysis cells (MECs) and found that the presence of NH4+, which can inhibit the activity of the nitrogenase enzyme, did not significantly reduce N2 fixation rates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, ORCID, Web Of Science
Added: February 5, 2019

2019 journal article

Public questions spur the discovery of new bacterial species associated with lignin bioconversion of industrial waste

ROYAL SOCIETY OPEN SCIENCE, 6(3).

By: S. Mathews*, M. Epps*, R. Blackburn n, M. Goshe n, A. Grunden n & R. Dunn n

author keywords: greenhouse camel cricket; hide beetle; Cedecea lapagei; lignocellulose; secretome
TL;DR: This work demonstrates a novel source of lignocellulose-degrading bacteria and introduces an effective workflow to identify bacterial enzymes for transforming industrial waste into value-added products and suggests the value of ecologically guided discovery of novel organisms. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: May 28, 2019

2018 review

Bacteria and archaea as the sources of traits for enhanced plant phenotypes

[Review of ]. BIOTECHNOLOGY ADVANCES, 36(7), 1900–1916.

By: C. Smith-Moore n & A. Grunden n

author keywords: Abiotic Stress; Archaea; Bacteria; Carbon Fixation; Genetic Engineering; Nitrogen Acquisition; Phosphate Acquisition; Yield
MeSH headings : Archaea / genetics; Bacteria / genetics; Carbon Cycle; Genetic Engineering; Nitrogen / metabolism; Phenotype; Phosphates / metabolism; Photosynthesis; Plant Development; Plant Physiological Phenomena; Plants / genetics; Plants / microbiology; Plants, Genetically Modified; Stress, Physiological
TL;DR: Transgenic plants expressing bacterial and archaeal genes will be discussed along with emerging strategies and tools to increase plant growth and yield. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: October 16, 2018

2018 journal article

Cloning, Over-Expression, and Purification of Carbonic Anhydrase from an Extremophilic Bacterium: An Introduction to Advanced Molecular Biology

AMERICAN BIOLOGY TEACHER, 80(1), 29–34.

By: V. Somalinga*, H. Klemmer n, A. Arun n, S. Mathews*, H. Wapshott n & A. Grunden n

author keywords: research methods; high school student research program; carbonic anhydrase; protein purification; protonography; Photobacterium profundum
TL;DR: Successful cloning and over-expression by high school students of two novel carbonic anhydrases from a psychrohalophile with application in biofuel production are reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2018 review

Methods for enhancing cyanobacterial stress tolerance to enable improved production of biofuels and industrially relevant chemicals

[Review of ]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 102(4), 1617–1628.

By: R. Kitchener n & A. Grunden n

author keywords: Cyanobacteria; Biofuel; Stress tolerance; Stress response; Stress mitigation
MeSH headings : Alcohols / metabolism; Alcohols / toxicity; Alkanes / metabolism; Alkanes / toxicity; Biofuels / toxicity; Cyanobacteria / drug effects; Cyanobacteria / genetics; Cyanobacteria / physiology; Drug Tolerance; Ethylenes / metabolism; Ethylenes / toxicity; Fatty Acids / metabolism; Fatty Acids / toxicity; Genetic Engineering / methods; Industrial Microbiology / methods; Stress, Physiological
TL;DR: Genetic manipulation, directed evolution, and supplementation of culture media with antioxidants are all viable strategies for designing more robust cyanobacterial strains that have the potential to meet industrial production goals. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Construction and setup of a bench-scale algal photosynthetic bioreactor with temperature, light, and ph monitoring for kinetic growth tests

Jove-Journal of Visualized Experiments, (124).

By: A. Karam, C. McMillan, Y. Lai, F. Reyes, H. Sederoff, A. Grunden, R. Ranjithan, J. Levis, J. Ducoste

Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

Escherichia coli O157:H7 Acid Sensitivity Correlates with Flocculation Phenotype during Nutrient Limitation

FRONTIERS IN MICROBIOLOGY, 8.

By: K. Kay n, F. Breidt n, P. Fratamico*, G. Baranzoni*, G. Kim*, A. Grunden n, D. Oh*

author keywords: STEC; acid resistance; nutrient limitation; curli; GcvB
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Revisiting Graduate Student Training to Address Agricultural and Environmental Societal Challenges

Agricultural & Environmental Letters, 2(1), 170019.

By: O. Duckworth n, M. Andrews n, M. Cubeta n, A. Grunden n & P. Ojiambo n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2016 journal article

A High-Resolution Crystal Structure of a Psychrohalophilic alpha-Carbonic Anhydrase from Photobacterium profundum Reveals a Unique Dimer Interface

PLOS ONE, 11(12).

By: V. Somalinga n, G. Buhrman n, A. Arun n, R. Rose n & A. Grunden n

Ed(s): A. Hofmann

MeSH headings : Carbonic Anhydrases / chemistry; Chlorides / chemistry; Chromatography, Gel; Crystallography, X-Ray; Dimerization; Molecular Dynamics Simulation; Photobacterium / enzymology; Protein Conformation
TL;DR: The first crystal structure of a pyschrohalophilic α-CA from a deep-sea bacterium, Photobacterium profundum, is reported and reveals a highly conserved “knot-topology” that is characteristic of α–CA’s. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain

Applied Microbiology and Biotechnology, 101(4), 1615–1630.

By: J. Whitham n, M. Schulte n, B. Bobay*, J. Bruno-Barcena n, M. Chinn n, M. Flickinger n, J. Pawlak n, A. Grunden n

Contributors: J. Whitham n, M. Schulte n, B. Bobay*, J. Bruno-Barcena n, M. Chinn n, M. Flickinger n, J. Pawlak n, A. Grunden n

author keywords: Acetyl-coA; Carbon monoxide dehydrogenase; Clostridium ljungdahlii; Ethanol; Wood-Ljungdahl pathway
MeSH headings : Acetyl Coenzyme A / metabolism; Aldehyde Oxidoreductases / metabolism; Carbon Dioxide / metabolism; Carbon Monoxide / metabolism; Clostridium / metabolism; Ethanol / metabolism; Multienzyme Complexes / metabolism
TL;DR: A non-autotrophic hyper ethanol-producing strain of C. ljungdahlii was identified that has utility for further physiology and strain performance studies and as a biocatalyst for industrial applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2016 journal article

Degradation of lignocellulose and lignin by Paenibacillus glucanolyticus

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 110, 79–86.

By: S. Mathews n, A. Grunden n & J. Pawlak n

author keywords: Lignin degradation; Lignocellulose degradation; Paenibacillus glucanolyticus; Bioproducts
TL;DR: Results show that both strains of P. glucanolyticus can degrade aromatic lignin-related compounds under aerobic and anaerobic conditions, however, only P. glucose SLM1 can also degrade polymeric lign in under aerobic conditions. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Enhancing Heat Tolerance of the Little Dogwood Cornus canadensis L. f. with Introduction of a Superoxide Reductase Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus

Frontiers in Plant Science, 7.

By: X. Geng n, X. Liu n, M. Ji n, W. Hoffmann n, A. Grunden n & Q. Xiang n

author keywords: antioxidant enzyme; Cornus canadensis; genetic transformation; heat tolerance; Pyrococcus furiosus; reactive oxygen species (ROS); superoxide reductase (SOR)
TL;DR: This is the first report of the successful demonstration of improved heat tolerance in a non-model plant resulting from the introduction of P. furiosus SOR, and demonstrates the potential of SOR for crop improvement and that inherent limitations of plant heat tolerance can be ameliorated with P.furiosu SOR. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2016 journal article

Microbial diversity of extreme habitats in human homes

PeerJ, 4(9), e2376.

By: A. Savage*, J. Hills*, K. Driscoll, D. Fergus*, A. Grunden n & R. Dunn n

Contributors: A. Savage*, J. Hills*, K. Driscoll, D. Fergus*, A. Grunden n & R. Dunn n

author keywords: Community Ecology; Extreme environments; Human Homes; Interactive effects; Microbialdiversity; Temperature; pH; Chemical
TL;DR: High-throughput sequencing techniques are used to assess bacterial and archaeal diversity in the extreme environments inside human homes with extreme temperature, pH, and chemical environmental conditions, finding a lower diversity of microbes in these extreme home environments compared to less extreme habitats in the home. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2016 journal article

Purification and characterization of a recombinant laccase-like multi-copper oxidase from Paenibacillus glucanolyticus SLM1

JOURNAL OF APPLIED MICROBIOLOGY, 121(5), 1335–1345.

By: S. Mathews n, C. Smithson* & A. Grunden n

author keywords: dye decolorization; laccase; lignin degradation; Paenibacillus glucanolyticus SLM1; recombinant expression
MeSH headings : Benzothiazoles / metabolism; Catechols / metabolism; Coloring Agents / metabolism; Hydrogen-Ion Concentration; Oxidoreductases / chemistry; Oxidoreductases / genetics; Oxidoreductases / isolation & purification; Oxidoreductases / metabolism; Paenibacillus / enzymology; Pyrogallol / analogs & derivatives; Pyrogallol / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sulfonic Acids / metabolism
TL;DR: The aim of this study was to evaluate the activity of a novel bacterial laccase‐like multi‐copper oxidase (LMCO) from Paenibacillus glucanolyticus SLM1: a bacterium isolated from pulp and paper waste. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants

PLASMID, 81, 55–62.

By: J. Dalal n, R. Yalamanchili n, C. Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n ...

Contributors: J. Dalal n, R. Yalamanchili n, C. La Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n, H. Sederoff n, R. Qu n

author keywords: Gateway; Plant transformation; Agrobacterium; Gene stacking
MeSH headings : Cloning, Molecular / methods; Gene Expression; Gene Order; Genes, Reporter; Genetic Vectors / genetics; Plants, Genetically Modified; Plasmids / genetics; Transformation, Genetic; Transgenes
TL;DR: The PC-GW vectors provide choices for selectable markers, cloning methods, and can accommodate up to eight gene constructs in a single T-DNA, thereby significantly reducing the number of transformations or crosses needed to generate multi-transgene expressing plants. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Bacterial biodegradation and bioconversion of industrial lignocellulosic streams

Applied Microbiology and Biotechnology, 99(7), 2939–2954.

By: S. Mathews n, J. Pawlak n & A. Grunden n

author keywords: Bacteria; Biodegradation; Cellulose; Hemicellulose; Lignin; Lignocellulose
MeSH headings : Bacteria / growth & development; Bacteria / metabolism; Biodegradation, Environmental; Biotechnology / methods; Cellulose / chemistry; Cellulose / metabolism; Enzymes / genetics; Enzymes / metabolism; Fungi / metabolism; Industrial Waste; Lignin / chemistry; Lignin / metabolism; Polysaccharides / chemistry; Polysaccharides / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Waste Management / methods
TL;DR: Treatment of underutilized streams of materials that contain cellulose, hemicellulose, and lignin with bacteria, which specifically degrade lignocellulose through the action of enzymes, offers a low-energy and low-cost method for biodegradation and bioconversion. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Characterization of recombinant glutathione reductase from the psychrophilic Antarctic bacterium Colwellia psychrerythraea

EXTREMOPHILES, 19(4), 863–874.

By: M. Ji n, C. Barnwell n & A. Grunden n

author keywords: Colwellia psychrerythraea; Glutathione reductase; Psychrophile; Oxidative stress; Antioxidant enzyme
MeSH headings : Alteromonadaceae / enzymology; Alteromonadaceae / genetics; Antarctic Regions; Bacterial Proteins / biosynthesis; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Glutathione Reductase / biosynthesis; Glutathione Reductase / chemistry; Glutathione Reductase / genetics; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics
TL;DR: A recombinant form of glutathione reductase from Colwellia psychrerythraea, a marine psychrophilic bacterium, has been biochemically characterized to determine its molecular and enzymatic properties, suggesting its potential utility as an antioxidant enzyme in heterologous systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Metabolic Response of Clostridium ljungdahlii to Oxygen Exposure

Applied and Environmental Microbiology, 81(24), 8379–8391.

By: J. Whitham n, O. Tirado-Acevedo n, M. Chinn n, J. Pawlak n & A. Grunden n

Ed(s): R. Parales

MeSH headings : Acetates / metabolism; Aldehyde Oxidoreductases / metabolism; Base Sequence; Biofuels / microbiology; Bioreactors / microbiology; Carbon Dioxide / metabolism; Clostridium / enzymology; Clostridium / growth & development; Clostridium / metabolism; DNA, Bacterial / genetics; Energy Metabolism / physiology; Ethanol / metabolism; Fermentation / physiology; Gene Expression / drug effects; Molecular Sequence Data; Oxidation-Reduction / drug effects; Oxidoreductases / metabolism; Oxygen / metabolism; Oxygen / pharmacology; Reactive Oxygen Species / metabolism; Sequence Alignment; Sequence Analysis, DNA
TL;DR: The metabolic responses of higher ethanol production and oxygen/ROS detoxification were found to be linked by cofactor management and substrate and energy metabolism and new genetic targets to generate C. ljungdahlii strains that produce more ethanol and are more tolerant to syngas contaminants are provided. (via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies

Scientific Data, 2(1).

MeSH headings : Algorithms; Clostridium / genetics; Genome, Bacterial; Genomics; Sequence Analysis, DNA / methods
TL;DR: High quality sequence datasets which span three generations of sequencing technologies, containing six types of data from four NGS platforms and originating from a single microorganism, Clostridium autoethanogenum are described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Crossref, ORCID
Added: March 4, 2019

2014 review

Biotechnological applications of halophilic lipases and thioesterases

[Review of ]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(3), 1011–1021.

By: S. Schreck n & A. Grunden n

author keywords: Lipase; Thioesterase; Halophilic; Halotolerant; Biofuel; Algae; Dunaliella
MeSH headings : Biotechnology / methods; Lipase / metabolism; Thiolester Hydrolases / metabolism
TL;DR: This review provides a brief overview of lipases and esterases and examines specific structural motifs and evolutionary adaptations of halophilic lipases, and provides an in-depth look at an exciting algal-based biofuel production system. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Isolation of Paenibacillus glucanolyticus from pulp mill sources with potential to deconstruct pulping waste

BIORESOURCE TECHNOLOGY, 164, 100–105.

By: S. Mathews n, J. Pawlak n & A. Grunden n

author keywords: Biodegradation (bacterial degradation); Pulp mill waste; Black liquor; Lignin; Paenibacillus glucanolyticus
MeSH headings : Anaerobiosis / drug effects; Biomass; Carbon / pharmacology; Fermentation / drug effects; Gas Chromatography-Mass Spectrometry; Hydrogen-Ion Concentration; Industrial Waste; Lignin / metabolism; Paenibacillus / genetics; Paenibacillus / growth & development; Paenibacillus / isolation & purification; Paper; RNA, Ribosomal, 16S / genetics; Temperature
TL;DR: Minimal media growth curves demonstrated that this facultative anaerobic microorganism can degrade black liquor as well as cellulose, hemicellulose, and lignin and was shown to be capable of growth onblack liquor as the sole carbon source based on minimal media growth studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

ASI:Dunaliellamarine microalgae to drop-in replacement liquid transportation fuel

Environmental Progress & Sustainable Energy, 32(4), 916–925.

By: W. Wang n, E. Allen n, A. Campos n, R. Cade n, L. Dean*, M. Dvora n, J. Immer n, S. Mixson n ...

Contributors: W. Wang n, E. Allen n, A. Campos n, R. Cade n, L. Dean*, M. Dvora n, J. Immer n, S. Mixson n ...

TL;DR: A technically feasible and scalable thermo-catalytic process to convert the lipids into replacements for liquid transportation fuels has been developed and these alkanes can be reformed into renewable diesel via conventional catalytic hydrocarbon isomerization reactions to improve cold flow properties, if desired. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: March 1, 2020

2012 journal article

A versatile method for preparation of hydrated microbial–latex biocatalytic coatings for gas absorption and gas evolution

Journal of Industrial Microbiology & Biotechnology, 39(9), 1269–1278.

By: J. Gosse n, M. Chinn n, A. Grunden n, O. Bernal n, J. Jenkins n, C. Yeager*, S. Kosourov*, M. Seibert*, M. Flickinger n

author keywords: Latex coating immobilization on chromatography paper; Chlamydomonas; Rhodopseudomonas; Clostridium; Synechococcus
MeSH headings : Absorption; Bacterial Adhesion; Biocatalysis; Bioreactors; Carbon Dioxide / metabolism; Carbon Monoxide / metabolism; Gases / chemistry; Gases / metabolism; Hydrogen / metabolism; Latex / chemistry; Oxygen / metabolism; Paper; Rhodopseudomonas / growth & development; Rhodopseudomonas / metabolism
TL;DR: The immobilization of different microorganisms in thin adhesive films in the gas phase demonstrates the utility of this method for evaluating genetically optimized microorganisms for gas absorption and gas evolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2012 journal article

Evaluation of a quantitative H2S MPN test for fecal microbes analysis of water using biochemical and molecular identification

WATER RESEARCH, 46(6), 1693–1704.

By: L. McMahan*, A. Grunden n, A. Devine* & M. Sobsey*

author keywords: Quantitative H2S test; Microbial water quality; TRFLP; Fecal indicator
MeSH headings : Bacteria / classification; Bacteria / genetics; Bacteria / isolation & purification; Cell Culture Techniques; DNA, Bacterial / genetics; DNA, Bacterial / isolation & purification; Environmental Monitoring / methods; Feces / microbiology; Genome, Bacterial / genetics; Hydrogen Sulfide / pharmacology; Lakes / microbiology; North Carolina; Polymorphism, Restriction Fragment Length; Rain / microbiology; Software; Software Design; Species Specificity; Universities; Water; Water Microbiology; Water Wells / microbiology
TL;DR: Overall, it was shown that water samples testing positive for H(2)S bacteria also had bacteria of likely fecal origin and waters containing fecal pathogens also were positive for the test, further document the validity of the H( 2)S test for detecting and quantifying fecal contamination of water. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 review

Prolidase function in proline metabolism and its medical and biotechnological applications

[Review of ]. JOURNAL OF APPLIED MICROBIOLOGY, 113(2), 233–247.

By: R. Kitchener n & A. Grunden n

author keywords: biotechnology; degradation; diagnosis; enzymes; peptides
MeSH headings : Animals; Archaea / enzymology; Bacteria / enzymology; Biodegradation, Environmental; Biotechnology; Collagen / metabolism; Dipeptidases / deficiency; Dipeptidases / metabolism; Dipeptidases / therapeutic use; Enzyme Replacement Therapy; Food Industry; Humans; Proline / metabolism; Protein Structure, Tertiary
TL;DR: The many in vivo functions of procaryotic and eucaryotic prolidases, as well as the most recent advances in therapeutic and biotechnological application of prolidase are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

Archaea, 2011, 1–9.

By: C. Theriot*, R. Semcer n, S. Shah & A. Grunden n

Ed(s): B. C.M. & S. Shah

MeSH headings : Biotransformation; Chemical Warfare Agents / metabolism; Dipeptidases / genetics; Dipeptidases / metabolism; Enzyme Stability; Hydrogen-Ion Concentration; Mutant Proteins / genetics; Mutant Proteins / metabolism; Mutation; Organophosphates / metabolism; Pyrococcus horikoshii / chemistry; Pyrococcus horikoshii / enzymology; Pyrococcus horikoshii / metabolism; Sarin / metabolism; Soman / metabolism; Temperature
TL;DR: Four Ph1prol mutants were isolated which had greater thermostability and improved activity over a broader range of temperatures against Xaa-Pro dipeptides and OP nerve agents compared to wild type Pyrococcus prolidases. (via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2011 journal article

Validation of the H2S method to detect bacteria of fecal origin by cultured and molecular methods

Applied Microbiology and Biotechnology, 92(6), 1287–1295.

By: L. McMahan*, A. Devine*, A. Grunden n & M. Sobsey*

author keywords: H2S test; TRFLP; Microbial water quality
MeSH headings : Amplified Fragment Length Polymorphism Analysis / methods; Bacteria / chemistry; Bacteria / genetics; Bacteria / isolation & purification; Bacteria / metabolism; Biochemistry / methods; Feces / microbiology; Hydrogen Sulfide / analysis; Hydrogen Sulfide / metabolism; Polymorphism, Restriction Fragment Length; Water Microbiology; Water Pollution / analysis
TL;DR: This research determined whether or not the H2S test did correctly identify sewage-contaminated waters by being the first to use culturing and molecular methods to identify the types and numbers of fecal indicator organisms, pathogens, and other microbes present in sewage samples with positive H1N1 test results. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, ORCID
Added: September 8, 2020

2010 journal article

Basal Signaling Regulates Plant Growth and Development

PLANT PHYSIOLOGY, 154(2), 439–443.

By: W. Boss n, H. Sederoff n, Y. Im n, N. Moran n, A. Grunden n & I. Perera n

Contributors: W. Boss n, H. Sederoff n, Y. Im n, N. Moran n, A. Grunden n & I. Perera n

MeSH headings : Plant Development; Plant Physiological Phenomena; Second Messenger Systems
TL;DR: The term signal transduction refers to the classical paradigm where an external stimulus is sensed and initiates an increase in second messengers and each second messenger transmits and amplifies the signal by activating a subset of downstream pathways. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Hydrolysis of organophosphorus compounds by microbial enzymes

Applied Microbiology and Biotechnology, 89(1), 35–43.

By: C. Theriot n & A. Grunden n

author keywords: Organophosphorus compound; OP nerve agent; Pesticide; OPAA; OPH; Phosphotriesterase; Prolidase
MeSH headings : Aryldialkylphosphatase / chemistry; Aryldialkylphosphatase / genetics; Aryldialkylphosphatase / metabolism; Bacteria / chemistry; Bacteria / enzymology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biocatalysis; Hydrolysis; Kinetics; Organophosphorus Compounds / chemistry; Substrate Specificity
TL;DR: An update of what is experimentally known about OPH and OPAA to include their structures, substrate specificity, and catalytic properties is provided. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2010 journal article

Improving the catalytic activity of hyperthermophilic Pyrococcus prolidases for detoxification of organophosphorus nerve agents over a broad range of temperatures

Applied Microbiology and Biotechnology, 87(5), 1715–1726.

By: C. Theriot n, X. Du n, S. Tove n & A. Grunden n

author keywords: Prolidase; Pyrococcus furiosus; OP nerve agents; Mutagenesis; Directed evolution
MeSH headings : Amino Acid Substitution / genetics; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; Chemical Warfare Agents / metabolism; Culture Media / chemistry; Dipeptidases / chemistry; Dipeptidases / genetics; Dipeptidases / isolation & purification; Dipeptidases / metabolism; Enzyme Stability; Escherichia coli / genetics; Models, Molecular; Mutagenesis; Organophosphorus Compounds / metabolism; Pesticides / metabolism; Plasmids; Polymerase Chain Reaction / methods; Protein Stability; Protein Structure, Tertiary; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Sequence Deletion; Temperature
TL;DR: To obtain a better enzyme for OP nerve agent decontamination and to investigate structural factors that influence protein thermostability and thermoactivity, randomly mutated P. furiosus prolidase mutants with improved activity over a broader range of temperatures were isolated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2010 review

Production of biofuels from synthesis gas using microbial catalysts

[Review of ]. Advances in applied microbiology, vol 70, 70, 57–92.

By: O. Tirado-Acevedo, M. Chinn & A. Grunden

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii

Applied Microbiology and Biotechnology, 86(1), 177–188.

By: C. Theriot n, S. Tove n & A. Grunden n

author keywords: Prolidase; Pyrococcus horikoshii; Hyperthermophile; Metalloenzyme; Cobalt enzyme
MeSH headings : Amino Acid Sequence; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Biotechnology / methods; Catalytic Domain; Cobalt / metabolism; Dipeptidases / chemistry; Dipeptidases / genetics; Dipeptidases / metabolism; Enzyme Stability; Hot Temperature; Hydrogen-Ion Concentration; Molecular Sequence Data; Pyrococcus horikoshii / enzymology; Pyrococcus horikoshii / genetics; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR: Biochemical characterization of two thermostable prolidases identified in the genome of Pyrococcus horikoshii shows they have higher catalytic activities over a broader pH range, higher affinity for metal and are more stable compared to P. furiosus prolidase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2009 journal article

Effects of antibiotics and oil on microbial profiles and fermentation in mixed cultures of ruminal microorganisms

JOURNAL OF DAIRY SCIENCE, 92(9), 4467–4480.

By: M. Johnson*, A. Devine n, J. Ellis, A. Grunden n & V. Fellner*

author keywords: ionophore; soybean oil; rumen microbial diversity; continuous culture
MeSH headings : Ammonia / metabolism; Animals; Anti-Bacterial Agents / pharmacology; Bacteria / classification; Bacteria / drug effects; Biodiversity; Bioreactors; Cattle; Fatty Acids, Volatile / metabolism; Female; Fermentation / drug effects; Hydrogen-Ion Concentration; In Vitro Techniques; Methane / metabolism; Rumen / microbiology; Soybean Oil / pharmacology
TL;DR: The terminal restriction fragment length polymorphism data suggest that microbial adaptation to the in vitro system in the control fermentor occurred within 4 d, and in contrast, treatments MO, OM, BO, and OB had little effect on culture fermentation despite differences in the patterns of microbial fragments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Erratum to: Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii

Applied Microbiology and Biotechnology, 86(1), 393–393.

By: C. Theriot n, S. Tove n & A. Grunden n

TL;DR: Fig. 2 Activity of P. horikoshii prolidases over a pH range of 4.0–10.0 shows 100% specific activity corresponds to 2,321 U/mg for Phprol and 3,357 U/ mg for Ph1prol. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 21, 2021

2009 journal article

Ethanol and acetate production by Clostridium ljungdahlii and Clostridium autoethanogenum using resting cells

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 32(3), 369–380.

By: J. Cotter n, M. Chinn n & A. Grunden n

author keywords: Clostridium ljungdahlii; Clostridium autoethanaogenum; Ethanol; Acetate; Resting cells; Non-growing cells
MeSH headings : Acetates / metabolism; Cell Culture Techniques / methods; Clostridium / chemistry; Clostridium / classification; Clostridium / growth & development; Clostridium / metabolism; Ethanol / metabolism; Hydrogen-Ion Concentration; Nitrogen / metabolism; Species Specificity
TL;DR: Clostridium autoethanogenum cultures were not induced into true resting states but did show improvement in total ethanol production as well as increased shifts in ethanol-to-acetate production ratios, and resting cell states were induced in autotrophic Clostridia ljungdahlii cultures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Expression of Pyrococcus furiosus Superoxide Reductase in Arabidopsis Enhances Heat Tolerance

PLANT PHYSIOLOGY, 151(2), 893–904.

By: Y. Im n, M. Ji n, A. Lee n, R. Killens n, A. Grunden n & W. Boss n

MeSH headings : Adaptation, Physiological; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Ascorbate Peroxidases; Chlorophyll / metabolism; Gene Expression Regulation, Plant; Germination; Heat-Shock Response; Hot Temperature; Hydrogen Peroxide / metabolism; Immunoprecipitation; Oxidoreductases / genetics; Oxidoreductases / metabolism; Peroxidases / metabolism; Plant Extracts / metabolism; Plant Leaves / metabolism; Plant Roots / metabolism; Plants, Genetically Modified; Pyrococcus furiosus / enzymology; RNA, Messenger / genetics; RNA, Messenger / metabolism; Seedlings / enzymology; Seeds / metabolism
TL;DR: It is shown that P. furiosus SOR can be produced as a functional enzyme in planta and that plants producing SOR have enhanced tolerance to heat, light, and chemically induced ROS. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Influence of process parameters on growth of Clostridium ljungdahlii and Clostridium autoethanogenum on synthesis gas

ENZYME AND MICROBIAL TECHNOLOGY, 44(5), 281–288.

By: J. Cotter n, M. Chinn n & A. Grunden n

author keywords: Clostridium ljungdahlii; Clostridium autoethanaogenum; Synthesis gas fermentation; Ethanol; Acetate
TL;DR: Higher flow rates resulted in greater end product formation with no significant effect on product ratios in C. autoethanogenum fermentations, and the ethanol to acetate ratios were smaller at lower pH levels and higher flow rates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Molecular characterization of the recombinant iron-containing alcohol dehydrogenase from the hyperthermophilic Archaeon, Thermococcus strain ES1

EXTREMOPHILES, 13(2), 299–311.

By: X. Ying*, A. Grunden n, L. Nie*, M. Adams* & K. Ma*

author keywords: Iron-containing alcohol dehydrogenase; Heterologous expression; Hyperthermophile; Archaea; Thermococcus
MeSH headings : Alcohol Dehydrogenase / chemistry; Amino Acid Sequence; Archaea; Cloning, Molecular; Hydrogen-Ion Concentration; Iron / chemistry; Kinetics; Molecular Sequence Data; NADP / metabolism; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Temperature; Thermococcus / metabolism
TL;DR: The gene encoding a thermostable iron-containing alcohol dehydrogenase from Thermococcus Strain ES1 was cloned, sequenced and expressed in Escherichia coli and it was concluded that the gene encoding ES1 ADH was expressed successfully in E. coli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Proteomics of Pyrococcus furiosus, a Hyperthermophilic Archaeon Refractory to Traditional Methods

JOURNAL OF PROTEOME RESEARCH, 8(8), 3844–3851.

By: A. Lee n, J. Sevinsky n, J. Bundy n, A. Grunden n & J. Stephenson n

author keywords: Pyrococcus furiosus; shotgun proteomics; archaea; hyperthermophile; high throughput; IPG-IEF; LC-MS/MS; archaeal proteins; microwave assisted acid hydrolysis
MeSH headings : Archaeal Proteins / analysis; Archaeal Proteins / metabolism; Hydrogen-Ion Concentration; Hydrolysis; Isoelectric Point; Microwaves; Peptide Fragments / analysis; Peptide Fragments / metabolism; Peptide Mapping; Protein Conformation; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Pyrococcus furiosus / chemistry; Trypsin / metabolism
TL;DR: A simple shotgun proteomics approach for the global identification of the P. furiosus proteome using a detergent-based microwave assisted acid hydrolysis step coupled with an overnight trypsin digest to obtain peptides is developed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 review

biotechnological applications of recombinant microbial prolidases

[Review of ]. Advances in applied microbiology, vol 68, 68, 99-.

By: C. Theriot, S. Tove & A. Grunden

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Characterization of the dinuclear metal center of Pyrococcus furiosus prolidase by analysis of targeted mutants

FEBS LETTERS, 579(27), 6140–6146.

By: X. Du*, S. Tove, K. Kast-Hutcheson & A. Grunden*

author keywords: prolidase; aminopeptidase; dinuclear metal center; cobalt enzyme; Pyrococcus furiosus
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Binding Sites; Catalysis; Cobalt / chemistry; Dipeptidases / chemistry; Dipeptidases / genetics; Mutation; Pyrococcus furiosus / enzymology
TL;DR: Metal‐content, enzyme activity and CD‐spectra analyses of D209A‐, H284L‐, and E327L‐prolidase mutants show that Co1 is the tight‐binding and Co2 the loose‐binding metal center. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

In vitro reconstitution of an NADPH-dependent superoxide reduction pathway from Pyrococcus furiosus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(3), 1522–1530.

By: A. Grunden n, F. Jenney*, K. Ma*, M. Ji n, M. Weinberg* & M. Adams*

MeSH headings : Amino Acid Sequence; Base Sequence; Biophysical Phenomena; Biophysics; DNA, Archaeal / genetics; Genes, Archaeal; Hydrogen Peroxide / metabolism; Kinetics; Molecular Sequence Data; NADH, NADPH Oxidoreductases / chemistry; NADH, NADPH Oxidoreductases / genetics; NADH, NADPH Oxidoreductases / metabolism; NADP / metabolism; Oxidation-Reduction; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sequence Homology, Amino Acid; Superoxides / metabolism
TL;DR: This is the first demonstration of in vitro superoxide reduction to hydrogen peroxide using NAD(P)H as the electron donor in an SOR-mediated pathway. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Production of a thermostable archaeal superoxide reductase in plant cells

FEBS LETTERS, 579(25), 5521–5526.

By: Y. Im n, M. Ji n, A. Lee n, W. Boss n & A. Grunden n

author keywords: archaeal; heat-stress; hyperthermophile; plant; reactive oxygen species; superoxide dismutase; superoxide reductase
MeSH headings : Archaeal Proteins / analysis; Archaeal Proteins / biosynthesis; Archaeal Proteins / genetics; Cells, Cultured; Cytoplasm / chemistry; Enzyme Stability; Green Fluorescent Proteins / analysis; Green Fluorescent Proteins / biosynthesis; Hot Temperature; Oxidoreductases / analysis; Oxidoreductases / biosynthesis; Oxidoreductases / genetics; Plants, Genetically Modified / cytology; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Pyrococcus furiosus / enzymology; Recombinant Fusion Proteins / analysis; Recombinant Fusion Proteins / biosynthesis; Superoxides / metabolism; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The Archaeal SOR provides a novel mechanism to reduce superoxide and demonstrates the potential for using archaeal genes to alter eukaryotic metabolism. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2004 journal article

Structure of the Prolidase fromPyrococcus furiosus†

Biochemistry, 43(10), 2771–2783.

By: M. Maher*, M. Ghosh*, A. Grunden*, A. Menon*, M. Adams*, H. Freeman*, J. Guss*

MeSH headings : Archaeal Proteins / antagonists & inhibitors; Archaeal Proteins / chemistry; Binding Sites; Cobalt / chemistry; Crystallization; Crystallography, X-Ray; Dimerization; Dipeptidases / antagonists & inhibitors; Dipeptidases / chemistry; Oligopeptides / chemistry; Proline / analogs & derivatives; Protease Inhibitors / chemistry; Pyrococcus furiosus / enzymology; Zinc / chemistry
TL;DR: The structure of prolidase from the hyperthermophilic archaeon Pyrococcus furiosus (Pfprol) has been solved and refined at 2.0 A resolution, and comparisons with the molecular structures of APPro and MetAP suggest how Pfprol discriminates against oligopeptides and in favor of Xaa-Pro substrates. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 24, 2021

2004 journal article

Synthesis/degradation ratio mass spectrometry for measuring relative dynamic protein turnover

ANALYTICAL CHEMISTRY, 76(1), 86–97.

By: B. Cargile n, J. Bundy n, A. Grunden n & J. Stephenson n

MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence / genetics; Mass Spectrometry / methods; Sequence Analysis, Protein / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: This technique is highly amenable to shotgun proteomics-based approaches and thus should allow relative turnover measurements for whole proteomes in the future, and requires only a single cell culture to obtain useful biological information about the processes occurring inside a cell. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2001 journal article

Molybdate transport

RESEARCH IN MICROBIOLOGY, 152(3-4), 311–321.

By: W. Self*, A. Grunden n, A. Hasona* & K. Shanmugam*

author keywords: molybdate transport; genetics; biochemistry; bacteria; archaea
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Bacterial Proteins / metabolism; Carrier Proteins / metabolism; Escherichia coli / metabolism; Escherichia coli Proteins / metabolism; Ion Transport; Molybdenum / metabolism; Multigene Family; Operon; Periplasmic Binding Proteins; Phylogeny
TL;DR: Comparative analysis of the molybdate transport proteins in various bacteria and archaea is the focus of this review. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2001 journal article

Proline dipeptidase from Pyrococcus furiosus

Hyperthermophilic Enzymes. Part A, 330, 433–445.

By: A. Grunden n, M. Ghosh & M. Adams

MeSH headings : Amino Acid Sequence; Base Sequence; DNA Primers; Dipeptidases / chemistry; Dipeptidases / isolation & purification; Dipeptidases / metabolism; Enzyme Stability; Molecular Sequence Data; Protein Conformation; Pyrococcus furiosus / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sequence Homology, Amino Acid
TL;DR: Prolyl residues are unique among the 20 common amino acid residues in that they confer a conformational constraint on peptide chains due to the cyclic nature of their pyrrolidine side group, which prevents cleavage of bonds adjacent to proline by most proteases; however, several enzymes have been characterized that are capable of hydrolyzing these bonds. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

Employment

Updated: December 30th, 2019 15:44

2000 - present

North Carolina State University Raleigh, NC, US
William Neal Reynolds Distinguished Professor of Microbiology Plant and Microbial Biology

Education

Updated: December 30th, 2019 15:47

1993 - 1996

University of Florida Gainesville, FL, US
PhD Microbiology and Cell Science

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