Adam Mischler

Works (6)

Updated: July 26th, 2023 21:17

2023 journal article

Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions

JOURNAL OF BIOLOGICAL CHEMISTRY, 299(5).

By: V. Karakis n, M. Jabeen n, J. Britt n, A. Cordiner  n, A. Mischler n, F. Li *, A. Miguel  n, B. Rao n 

Contributors: V. Karakis n, M. Jabeen n, J. Britt n, A. Cordiner  n, A. Mischler n, F. Li *, A. San Miguel n , B. Rao n 

MeSH headings : Humans; Cell Differentiation / drug effects; Colforsin / pharmacology; Colforsin / metabolism; HLA-G Antigens / genetics; HLA-G Antigens / metabolism; Laminin / pharmacology; Stem Cells / cytology; Stem Cells / drug effects; Transforming Growth Factor beta / metabolism; Trophoblasts / cytology; Trophoblasts / metabolism; Culture Media / chemistry; Hypoxia-Inducible Factor 1, alpha Subunit / genetics; Gene Expression Regulation, Developmental / drug effects; Cytological Techniques / methods
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5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 3, 2023

2021 journal article

Two distinct trophectoderm lineage stem cells from human pluripotent stem cells

JOURNAL OF BIOLOGICAL CHEMISTRY, 296.

By: A. Mischler n, V. Karakis n, J. Mahinthakumar  n, C. Carberry *, A. San Miguel n , J. Rager *, R. Fry *, B. Rao n 

Contributors: A. Mischler n, V. Karakis n, J. Mahinthakumar  n, C. Carberry *, A. Miguel  n, J. Rager *, R. Fry*, B. Rao n 

MeSH headings : CDX2 Transcription Factor / metabolism; Cell Culture Techniques; Cell Differentiation; Cell Lineage; Culture Media; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Female; Humans; Placenta / cytology; Placenta / metabolism; Pluripotent Stem Cells / cytology; Pluripotent Stem Cells / metabolism; Pregnancy; Trophoblasts / cytology; Trophoblasts / metabolism
TL;DR: Derivation of hTSCs and hTESCs from pluripotent stem cells significantly enables construction of models for normal and pathological placental development. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 7, 2021

2019 article

DERIVATION OF HUMAN TROPHOBLAST STEM CELLS FROM HUMAN PLURIPOTENT STEM CELLS

Mischler, A., Karakis, V., San Miguel, A., & Rao, B. (2019, August). PLACENTA, Vol. 83, pp. E59–E59.

By: A. Mischler n, V. Karakis n, A. San Miguel n  & B. Rao n 

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15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 20, 2020

2019 journal article

Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation

SCIENCE SIGNALING, 12(584).

By: K. Tiruthani n, A. Mischler n, S. Ahmed*, J. Mahinthakumar  n, J. Haugh n  & B. Rao n 

Contributors: K. Tiruthani n, A. Mischler n, S. Ahmed*, J. Mahinthakumar  n, J. Haugh n  & B. Rao n 

MeSH headings : Animals; Biosensing Techniques; Cell Line; Endocytosis; ErbB Receptors / genetics; ErbB Receptors / metabolism; Mice; Microscopy, Fluorescence; Phospholipase C gamma / genetics; Phospholipase C gamma / metabolism; Phosphorylation / genetics; src Homology Domains
TL;DR: The authors used two different mutagenesis and screening approaches to engineer new biosensors, mSH2 and SPY992, that exhibited greater specificity for EGFR Tyr992 and more accurately reported EGFR signaling kinetics, and could be extended to generate phospho-specific biosensor for various targets. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 24, 2019

2017 journal article

Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae

ACS SYNTHETIC BIOLOGY, 6(11), 2096–2107.

By: C. Cruz-Teran n, K. Tiruthani n, A. Mischler n & B. Rao n 

Contributors: C. Cruz-Teran n, K. Tiruthani n, A. Mischler n & B. Rao n 

author keywords: yeast surface display; secretion; ribosomal skipping; 2A peptide; Sso7d; glucose oxidase
MeSH headings : Cell Adhesion Molecules / genetics; Cell Adhesion Molecules / metabolism; Gene Expression; Peptide Library; Peptides / genetics; Peptides / metabolism; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism
TL;DR: A system for simultaneous cell surface display and soluble secretion of proteins in Saccharomyces cerevisiae based on inefficient ribosomal skipping is described and it is shown that binding proteins derived from the Sso7d scaffold and the homodimeric enzyme glucose oxidase can be simultaneously secreted solubly and displayed on the cell surface. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.

By: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n 

Contributors: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n 

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
TL;DR: The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

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