@article{joignant_knizner_xi_muddiman_2023, title={Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization}, volume={37}, ISSN={["1097-0231"]}, DOI={10.1002/rcm.9638}, abstractNote={RationaleInfrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) utilizes a 2970 nm mid‐IR laser to desorb samples with depth resolutions (Z) on the order of micrometers. Conventionally, 5–20 μm thick tissue sections are used to characterize different applications of the IR‐MALDESI source, but an optimal thickness has not been systematically investigated.}, number={22}, journal={RAPID COMMUNICATIONS IN MASS SPECTROMETRY}, author={Joignant, Alena N. and Knizner, Kevan T. and Xi, Ying and Muddiman, David C.}, year={2023}, month={Nov} }
 @article{joignant_xi_muddiman_2023, title={Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non-flat samples}, volume={3}, ISSN={["1096-9888"]}, DOI={10.1002/jms.4914}, abstractNote={Abstract}, journal={JOURNAL OF MASS SPECTROMETRY}, author={Joignant, Alena N. N. and Xi, Ying and Muddiman, David C. C.}, year={2023}, month={Mar} }
 @article{joignant_ritter_knizner_garrard_kullman_muddiman_2023, title={Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs)}, volume={8}, ISSN={["1879-1123"]}, DOI={10.1021/jasms.3c00210}, abstractNote={Increasing the spatial resolution of a mass spectrometry imaging (MSI) method results in a more defined heatmap of the spatial distribution of molecules across a sample, but it is also associated with the disadvantage of increased acquisition time. Decreasing the area of the region of interest to achieve shorter durations results in the loss of potentially valuable information in larger specimens. This work presents a novel MSI method to reduce the time of MSI data acquisition with variable step size imaging: nested regions of interest (nROIs). Using nROIs, a small ROI may be imaged at a higher spatial resolution while nested inside a lower-spatial-resolution peripheral ROI. This conserves the maximal spatial and chemical information generated from target regions while also decreasing the necessary acquisition time. In this work, the nROI method was characterized on mouse liver and applied to top-hat MSI of zebrafish using a novel optical train, which resulted in a significant improvement in both acquisition time and spatial detail of the zebrafish. The nROI method can be employed with any step size pairing and adapted to any method in which the acquisition time of larger high-resolution ROIs poses a practical challenge.}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY}, author={Joignant, Alena N. and Ritter, Morgan M. and Knizner, Kevan T. and Garrard, Kenneth P. and Kullman, Seth W. and Muddiman, David C.}, year={2023}, month={Aug} }
 @article{xi_sohn_joignant_cologna_prentice_muddiman_2023, title={SMART: A data reporting standard for mass spectrometry imaging}, volume={58}, ISSN={["1096-9888"]}, DOI={10.1002/jms.4904}, abstractNote={Abstract}, number={2}, journal={JOURNAL OF MASS SPECTROMETRY}, author={Xi, Ying and Sohn, Alexandria L. and Joignant, Alena N. and Cologna, Stephanie M. and Prentice, Boone M. and Muddiman, David C.}, year={2023}, month={Feb} }
 @article{joignant_bai_guymon_garrard_pankow_muddiman_2022, title={Developing transmission mode for infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging}, volume={36}, ISSN={["1097-0231"]}, DOI={10.1002/rcm.9386}, abstractNote={RationaleThe development and characterization of the novel NextGen infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) source catalyzed new advancements in IR‐MALDESI instrumentation, including the development of a new analysis geometry.}, number={22}, journal={RAPID COMMUNICATIONS IN MASS SPECTROMETRY}, author={Joignant, Alena N. and Bai, Hongxia and Guymon, Jacob P. and Garrard, Kenneth P. and Pankow, Mark and Muddiman, David C.}, year={2022}, month={Nov} }
 @article{joignant_bai_manni_muddiman_2022, title={Improved spatial resolution of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective}, volume={36}, ISSN={["1097-0231"]}, DOI={10.1002/rcm.9392}, abstractNote={RationaleThe level of visual detail of a mass spectrometry image is dependent on the spatial resolution with which it is acquired, which is largely determined by the focal diameter in infrared laser ablation‐based techniques. While the use of mid‐IR light for mass spectrometry imaging (MSI) has advantages, it results in a relatively large focal diameter and spatial resolution. The continual advancement of infrared matrix‐assisted electrospray ionization (IR‐MALDESI) for MSI warranted novel methods to decrease laser ablation areas and thus improve spatial resolution.}, number={23}, journal={RAPID COMMUNICATIONS IN MASS SPECTROMETRY}, author={Joignant, Alena N. and Bai, Hongxia and Manni, Jeffrey G., Sr. and Muddiman, David C.}, year={2022}, month={Dec} }