Arthur K. Weissinger

Works (22)

Updated: April 4th, 2024 22:25

2016 journal article

The Seed Biotinylated Protein of Soybean (Glycine max): A Boiling-Resistant New Allergen (Gly m 7) with the Capacity To Induce IgE-Mediated Allergic Responses

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 64(19), 3890–3900.

author keywords: Glycine max; Arachis hypogaea; biotin; allergenic orthologues
MeSH headings : Allergens / chemistry; Chromatography, Liquid; Hypersensitivity / immunology; Immunoglobulin E / immunology; Seeds / chemistry; Soybeans / chemistry; Soybeans / embryology; Tandem Mass Spectrometry
TL;DR: Soybean and peanut nSBPs may represent a novel class of biologically active legume allergens with the structural resilience to withstand many food-manufacturing processes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Analysis of CoQ(10) in Cultivated Tobacco by a High-Performance Liquid Chromatography-Ultraviolet Method

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(17), 9054–9058.

By: M. Stiff n, A. Weissinger n & D. Danehower n

author keywords: ubiquinone; coenzyme Q; tobacco; HPLC
MeSH headings : Chromatography, High Pressure Liquid / methods; Drug Stability; Plant Leaves / chemistry; Tobacco / anatomy & histology; Tobacco / chemistry; Ubiquinone / analogs & derivatives; Ubiquinone / analysis; Vitamins / analysis
TL;DR: A method for the routine analysis of coenzyme Q(10) in green leaf tissue of cultivated Nicotiana tabacum (tobacco) using high-performance liquid chromatography (HPLC) with UV detection is described, finding that this method will likely be useful in the analysis of CoQ in the green leaves of other plant species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 review

Hypoallergenic Legume Crops and Food Allergy: Factors Affecting Feasibility and Risk

[Review of ]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(1), 20–27.

By: J. Riascos n, A. Weissinger n, S. Weissinger n & A. Burks*

author keywords: Legume allergens; food allergies; hypoallergenic crops; soybean; peanut
MeSH headings : Arachis / genetics; Arachis / immunology; Fabaceae / genetics; Fabaceae / immunology; Food Hypersensitivity / immunology; Humans; Plant Proteins / genetics; Plant Proteins / immunology; Plants, Genetically Modified / genetics; Plants, Genetically Modified / immunology; Soybeans / genetics; Soybeans / immunology
TL;DR: Efforts to produce hypoallergenic cultivars of soybean and peanut are described and the challenges that need to be overcome before such products could be available in the marketplace are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 article

Amplicon-plus Targeting Technology (APTT) for rapid production of a highly unstable vaccine protein in tobacco plants (vol 64, pg 619, 2007)

Azhakanandam, K., Weissinger, S. M., Nicholson, J. S., Qu, R., & Weissinger, A. K. (2007, July). PLANT MOLECULAR BIOLOGY, Vol. 64, pp. 619–619.

By: K. Azhakanandam n, S. Weissinger n, J. Nicholson n, R. Qu n & A. Weissinger n

Source: Web Of Science
Added: August 6, 2018

2007 journal article

Amplicon-plus targeting technology (APTT) for rapid production of a highly unstable vaccine protein in tobacco plants

Plant Molecular Biology, 63(3), 393–404.

By: K. Azhakanandam n, S. Weissinger n, J. Nicholson n, R. Qu n & A. Weissinger n

MeSH headings : Biotechnology; Capsid Proteins / biosynthesis; Capsid Proteins / genetics; Capsid Proteins / immunology; Gene Expression; Genetic Vectors; Lambdapapillomavirus / genetics; Lambdapapillomavirus / immunology; Plants, Genetically Modified; RNA, Small Interfering / genetics; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Recombinant Proteins / immunology; Rhizobium / genetics; Tobacco / genetics; Vaccines, Subunit / biosynthesis; Vaccines, Subunit / genetics; Viral Vaccines / biosynthesis; Viral Vaccines / genetics
TL;DR: The good yield and short interval from inoculation to harvest characteristic of APTT, combined with the potential for high-throughput achieved by use of the agrospray inoculation protocol, make this system a very promising technology for producing high value recombinant proteins, especially those known to be highly labile, in plants for a wide range of applications. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes

MOLECULAR ECOLOGY, 14(10), 3177–3189.

By: M. Halfhill n, J. Sutherland*, H. Moon*, G. Poppy*, S. Warwick*, A. Weissinger n, T. Rufty n, P. Raymer*, C. Stewart*

author keywords: Bacillus thuringiensis; Brassica napus; Brassica rapa; hybridization; insect resistance; introgression
MeSH headings : Bacillus thuringiensis Toxins; Bacterial Proteins / genetics; Bacterial Toxins / genetics; Brassica napus / genetics; Brassica napus / growth & development; Brassica rapa / genetics; Brassica rapa / growth & development; Crosses, Genetic; Endotoxins / genetics; Green Fluorescent Proteins / genetics; Hemolysin Proteins; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Random Allocation; Triticum / growth & development
TL;DR: The hybrids were the least competitive with wheat compared with parental Brassica competitors, although differences between transgenic and nontransgenic hybrids varied with location. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to Tomato Spotted Wilt Virus in transgenic tobacco

TRANSGENIC RESEARCH, 14(2), 193–206.

By: J. Levin n, W. Thompson n, A. Csinos*, M. Stephenson* & A. Weissinger n

author keywords: gene silencing; matrix attachment regions; RNA-mediated virus resistance; Tomato Spotted Wilt Virus
MeSH headings : Breeding; DNA Methylation; Gene Expression Regulation; Gene Silencing; Immunity, Innate; Matrix Attachment Regions; Plants, Genetically Modified; Promoter Regions, Genetic; Tobacco / genetics; Tobacco / virology; Tospovirus / genetics; Tospovirus / pathogenicity; Transcription, Genetic
TL;DR: Results are consistent with the concept that MARs may prevent transcriptional silencing, and short interfering 21– 25 nt RNAs from the transgene that are characteristic of post-transcriptional gene silencing (PTGS) were present in the resistant R1 plants, but not the susceptible R4 plants, indicating that virus resistance was associated with PTGS of thetransgene. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2004 journal article

A chitinase from Tex6 maize kernels inhibits growth of Aspergillus flavus

PHYTOPATHOLOGY, 94(1), 82–87.

By: K. Moore, M. Price*, R. Boston*, A. Weissinger* & G. Payne*

TL;DR: The Tex6 chitinase purified in this study is capable of inhibiting the growth of A. flavus by 50% at a concentration of 20 mug/ml and makes a major contribution to the antifungal activity in this maize genotype. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions

Environmental Biosafety Research, 3(2), 73.

By: M. Halfhill*, W. Zhu Bin, P. Raymer, R. Millwood, A. Weissinger* & C. Stewart

MeSH headings : Agriculture; Bacillus thuringiensis / genetics; Bacillus thuringiensis / pathogenicity; Brassica napus / genetics; Brassica rapa / genetics; Green Fluorescent Proteins / genetics; Hybridization, Genetic; Plants, Genetically Modified; Raphanus / genetics; Risk Assessment; Safety; Seeds / genetics
TL;DR: Results show that transgene flow from many independent transformed lines of B. rapa can occur under a range of field conditions, and that transgenic hybrids have a high potential to produce transgenic seeds in backcrosses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

A multi-generation analysis of the stability of transgenic virus resistance in doubled-haploid tobacco lines

Molecular Breeding, 12(2), 145–156.

By: Y. Liu, J. Levin, J. Murray, E. Wernsman & A. Weissinger

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations

THEORETICAL AND APPLIED GENETICS, 107(8), 1533–1540.

By: M. Halfhill n, R. Millwood*, A. Weissinger n, S. Warwick* & C. Stewart*

author keywords: transgenic canola; Brassica rapa; green fluorescent protein; AFLPs; gene flow
MeSH headings : Crops, Agricultural / genetics; Crosses, Genetic; Green Fluorescent Proteins; Luminescent Proteins / genetics; Plants, Genetically Modified; Spectrometry, Fluorescence; Transgenes
TL;DR: It is demonstrated that the formation of homozygous individuals within hybrid populations increases the average level of transgene expression as generations progress, and this phenomenon must be considered in the development of risk-management strategies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Analysis of trans-silencing interactions using transcriptional silencers of varying strength and targets with and without flanking nuclear matrix attachment regions

TRANSGENIC RESEARCH, 12(3), 305–318.

By: R. Ascenzi n, B. Ulker n, J. Todd n, D. Sowinski n, C. Schimeneck n, G. Allen n, A. Weissinger n, W. Thompson n

author keywords: matrix attachment region; tobacco; transcriptional gene silencing; transgene expression; trans-silencing
MeSH headings : Gene Silencing; Inheritance Patterns; Matrix Attachment Regions / genetics; Seedlings / genetics; Sequence Homology; Silencer Elements, Transcriptional; Tobacco / genetics; Transcription, Genetic
TL;DR: The results are consistent with a model in which transcriptional silencing is triggered by transcription of sequences during the early stages of embryo or seedling development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2003 journal article

Spatial and temporal patterns of green fluorescent protein (GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L.

PLANT CELL REPORTS, 22(5), 338–343.

By: M. Halfhill n, R. Millwood*, T. Rufty n, A. Weissinger n & C. Stewart*

author keywords: green fluorescent protein; Bacillus thuringiensis; soluble protein concentration; transgene monitoring; canola
MeSH headings : Bacillus thuringiensis Toxins; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacterial Toxins; Brassica napus / genetics; Brassica napus / growth & development; Brassica napus / metabolism; Endotoxins / genetics; Endotoxins / metabolism; Fluorescence; Green Fluorescent Proteins; Hemolysin Proteins; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Plants, Genetically Modified / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism
TL;DR: The results show that GFP can be used as an accurate transgene marker but that aspects of plant developmental should be taken into account when interpreting fluorescence measurements. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2003 patent

Stable transformation of plant cells

Washington, DC: U.S. Patent and Trademark Office.

By: D. Tomes, A. Weissinger, J. Sanford & T. Klein

Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

A new form of crystalline rubisco and the conversion to its common dodecahedral form

JOURNAL OF PROTEOME RESEARCH, 1(5), 471–473.

By: P. Kwanyuen n, S. Allina n, A. Weissinger n & R. Wilson n

author keywords: rubisco; purification; crystallization; recrystallization; dodecahedron; fraction 2-protein
MeSH headings : Crystallography, X-Ray; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Protein Conformation; Ribulose-Bisphosphate Carboxylase / chemistry; Ribulose-Bisphosphate Carboxylase / isolation & purification; Temperature; Time Factors; Tobacco / metabolism
TL;DR: A new purification procedure is presented that yields a new crystalline form of rubisco and has enabled this most abundant protein from tobacco leaf extract to completely remove from fraction 2-protein. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

E-coli chromosomal DNA in a transgene locus created by microprojectile bombardment in tobacco

TRANSGENIC RESEARCH, 11(3), 311–313.

By: B. Ulker n, A. Weissinger n & S. Spiker n

author keywords: DNA purity; E. coli chromosomal DNA; microprojectile bombardment; transformation; transgene locus
MeSH headings : Biolistics; Escherichia coli / genetics; Plants, Genetically Modified; Tobacco / genetics; Transgenes
TL;DR: In the process of characterising the integration site of a transgene in the tobacco genome, a 260 base pair fragment of E. coli chromosomal DNA was discovered despite the fact that standard procedures were implemented to remove contaminating E. bacteria from the plasmid DNA used in the transformation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2001 patent

Stable transformation of plant cells

Washington, DC: U.S. Patent and Trademark Office.

By: D. Tomes, A. Weissinger, J. Sanford & T. Klein

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions

TRANSGENIC RESEARCH, 10(5), 465–470.

By: R. Ascenzi n, J. Ingram n, M. Massel n, W. Thompson n, S. Spiker n & A. Weissinger n

author keywords: cell differentiation and proliferation; chromatin; matrix; scaffold attachment region (MAR/SAR); plant high mobility group-I/Y protein (HMG-I/Y); transgene expression; tobacco
MeSH headings : Cell Differentiation; Cells, Cultured; Gene Expression; HMGA1a Protein / genetics; HMGA1a Protein / metabolism; Plants, Genetically Modified; Tobacco / cytology; Tobacco / genetics
TL;DR: It appears that tobacco nuclear matrix attachment region function is not enhanced simply because cells in culture divide rapidly and are not differentiated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

1999 journal article

A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants

PLANT JOURNAL, 18(3), 253–263.

By: B. Ulker, G. Allen*, W. Thompson*, S. Spiker* & A. Weissinger*

TL;DR: In primary transgenic plants, the average uidA expression in plants with MARs was twofold greater than in control plants without MARs, but there was no effect on variation of expression. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

1999 patent

Stable transformation of plant cells

Washington, DC: U.S. Patent and Trademark Office.

By: D. Tomes, A. Weissinger, J. Sanford & T. Klein

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Production and purification of an active bovine lysozyme in tobacco (Nicotiana tabacum): Utilization of value-added crop plants traditionally grown under intensive agriculture

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(7), 2793–2797.

By: C. Wilcox n, A. Weissinger n, R. Long n, L. Fitzmaurice n, T. Mirkov n & H. Swaisgood n

author keywords: transgenic; lysozyme; recombinant protein; antimicrobial; Nicotiana tabacum
TL;DR: It was concluded that tobacco was an excellent choice for expression of the recombinant protein and that the purification process developed in this study demonstrates methodology for isolation of high-value enzymes from tobacco and other crop plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1992 patent

Ballistic transformation of conifers

Washington, DC: U.S. Patent and Trademark Office.

By: A. Stomp, A. Weissinger & R. Sederoff

Source: NC State University Libraries
Added: August 6, 2018

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