@article{bissinger_apperson_sonenshine_watson_roe_2009, title={Efficacy of the new repellent BioUD(A (R)) against three species of ixodid ticks}, volume={48}, ISSN={["1572-9702"]}, DOI={10.1007/s10493-008-9235-x}, abstractNote={BioUD ® with the active ingredient 2-undecanone originally derived from wild tomato plants is a new repellent recently registered by the US EPA. Repellent efficacy of BioUD ® (7.75% 2-undecanone) and DEET (98.11%) was examined in the laboratory using a choice test between repellent-treated and control filter paper surfaces for Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis. BioUD ® provided greater repellency against A. americanum and I. scapularis than DEET. No difference was found between BioUD ® and DEET against D. variabilis. In head-to-head assays between BioUD ® and DEET, undiluted and 50% dilutions of BioUD® were more repellent than undiluted DEET against all three species tested. Similarly, a 25% dilution of BioUD® was more repellent than DEET against A. americanum while no difference in mean percentage repellency was found between a 25% dilution of BioUD® and DEET against I. scapularis. Based on regression analysis, the concentration of BioUD® required for equivalent repellency to 98.11% DEET was 39.5% for D. variabilis and 29.7% for I. scapularis. A log-probit model could not be constructed for A. americanum from the dosages tested. Based on filter paper head-to-head assays, BioUD® is at least 2–4 times more active as a repellent than DEET against three species of ixodid ticks under the conditions of our laboratory bioassays.}, number={3}, journal={EXPERIMENTAL AND APPLIED ACAROLOGY}, author={Bissinger, B. W. and Apperson, C. S. and Sonenshine, D. E. and Watson, D. W. and Roe, R. M.}, year={2009}, month={Jul}, pages={239–250} }
 @article{shen_brandt_witting-bissinger_gunnoe_roe_2009, title={Novel insecticide polymer chemistry to reduce the enzymatic digestion of a protein pesticide, trypsin modulating oostatic factor (TMOF)}, volume={93}, ISSN={["1095-9939"]}, DOI={10.1016/j.pestbp.2009.02.004}, abstractNote={A limiting factor in the use of proteins as insecticides, especially when the site of action is in the insect hemocoel, is protease degradation in the digestive system and hemolymph and movement across the midgut ventriculus. Trypsin modulating oostatic factor (TMOF) is a per os mosquito peptidic larvacide which moves across the digestive system and binds to receptors on the hemolymph side of the gut where the hormone inhibits protease synthesis and food utilization ultimately causing death. In the current study, the in vitro degradation of TMOF by the digestive enzyme, leucine aminopeptidase, was inhibited by conjugation of TMOF-K with aliphatic polyethylene glycol (PEG) polymers. Structure activity studies demonstrated a correlation between the molecular weight of the PEG polymer and resistance to digestion and show proof of concept that aliphatic-PEG protein polymerization can be used to prevent protease degradation of a protein insecticide.}, number={3}, journal={PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY}, author={Shen, Hongyan and Brandt, Alan and Witting-Bissinger, Brooke E. and Gunnoe, T. Brent and Roe, R. Michael}, year={2009}, month={Mar}, pages={144–152} }
 @article{witting-bissinger_orr_linker_2008, title={Effects of floral resources on fitness of the parasitoids Trichogramma exiguum (Hymenoptera: Trichogrammatidae) and Cotesia congregata (Hymenoptera: Braconidae)}, volume={47}, ISSN={["1090-2112"]}, DOI={10.1016/j.biocontrol.2008.07.013}, abstractNote={This study was conducted to determine if floral resources enhanced longevity and fecundity of the egg parasitoid Trichogramma exiguum Pinto & Platner and longevity of the larval parasitoid Cotesia congregata (Say). Newly eclosed (⩽12 h) female wasps were provisioned with fennel (Foeniculum vulgare P. Mill.) or buckwheat (Fagopyrum esculentum Moench) flowers or one of two controls: honey or water. Wasps were monitored daily until all had died. Daily egg production by T. exiguum was monitored using Ephestia kuehniella Keller egg cards. Longevity of both T. exiguum (6.7 d) and C. congregata (5.1 d) provided buckwheat flowers was increased approximately 8.5-fold compared with wasps provided only water. Buckwheat-provisioned T. exiguum exhibited 2-fold greater longevity than those provided fennel. Longevity of C. congregata provisioned with fennel and honey was not statistically different. Water-provisioned T. exiguum and C. congregata exhibited the shortest longevity (0.8 and 0.6 d, respectively). Total fecundity was 6.3-fold greater in T. exiguum provisioned with buckwheat and 2.3-fold greater in T. exiguum provisioned with fennel than in water controls. Average female to male ratio of progeny over the lifetime of each female was significantly greatest in T. exiguum provisioned with water alone, likely because of sperm depletion in wasps exhibiting greater longevity. Total mean number of female offspring produced was significantly greatest in T. exiguum provided honey or buckwheat flowers although no difference in total female offspring were observed between adults provisioned with buckwheat or fennel flowers. Our results show that provisioning T. exiguum with honey and buckwheat flowers resulted in greater longevity, total fecundity, and lifetime production of female offspring than water alone. Buckwheat flowers also lead to greater longevity in C. congregata.}, number={2}, journal={BIOLOGICAL CONTROL}, author={Witting-Bissinger, B. E. and Orr, D. B. and Linker, H. M.}, year={2008}, month={Nov}, pages={180–186} }
 @article{witting-bissinger_stumpf_donohue_apperson_roe_2008, title={Novel arthropod repellent, BioUD, is an efficacious alternative to deet}, volume={45}, ISSN={["1938-2928"]}, DOI={10.1603/0022-2585(2008)45[891:NARBIA]2.0.CO;2}, abstractNote={Abstract For >50 yr, N,N-diethyl-3-methylbenzamide (deet) has been the standard for arthropod repellents and has been an important tool to protect people from disease agents carried by ticks, mosquitoes, and other arthropods. However, some people avoid using deet because of concerns about adverse health effects. In 2007, a new repellent, BioUD, with the active ingredient 7.75% 2-undecanone, originally derived from wild tomato (Lycopersicon hirsutum Dunal f. glabratum C. H. Müll) plants, was registered by the U.S. Environmental Protection Agency. In the current study, repellent efficacy of BioUD was compared using arm-in-cage studies with 7 and 15% deet against the mosquitoes Aedes aegypti (L.) and Aedes albopictus Skuse. No differences were found in mean repellency over 6 h after application between BioUD versus 7 and 15% deet for Ae albopictus. For Ae. aegypti, no differences were found over the same time period for 7% deet. Compared with 15% deet, BioUD mean repellency was lower over the 6-h test period. Human subject field trials were conducted in North Carolina, United States, and Ontario, Canada, comparing the repellency of BioUD to products containing 25 and 30% deet. BioUD provided the same repellency or was more efficacious than 25 and 30% deet, respectively, in these studies. Laboratory trials were conducted to determine the repellent activity of BioUD against the American dog tick, Dermacentor variabilis (Say), on human skin and cloth. BioUD repelled ticks at least 2.5 h after application to human skin. On cloth, no differences in mean repellency were found through 8 d after application between BioUD and 7% deet. In a two-choice test for BioUD versus 15% deet on filter paper, ticks spent significantly more time on the deet-treated surface than the BioUD-treated surface. Based on these studies in toto, BioUD is an efficacious alternative to deet in its repellent activity.}, number={5}, journal={JOURNAL OF MEDICAL ENTOMOLOGY}, author={Witting-Bissinger, B. E. and Stumpf, C. F. and Donohue, K. V. and Apperson, C. S. and Roe, R. M.}, year={2008}, month={Sep}, pages={891–898} }
 @article{witting_orr_linker_2007, title={Attraction of insect natural enemies to habitat plantings in north carolina}, volume={42}, ISSN={["0749-8004"]}, DOI={10.18474/0749-8004-42.4.439}, abstractNote={Two studies were conducted to evaluate the relative attractiveness of floral habitats that may provide nectar and pollen food resources to natural enemies of agricultural insect pests in North Carolina. In the first study, timed observations were made of insect flower-feeding to estimate attraction of natural enemies to 16 plant species in 2004 and 5 plant species in 2005. Insects were identified to the family level and assigned to 1 of 6 feeding groups. In both years, crop predators fed from fennel (Foeniculum vulgare P. Mill.) flowers in greater abundance than from any other flowers observed. Crop parasitoids fed most often on fennel in 2005. In both years, the remaining insect feeding groups other than pollinators were not significantly affected by flower species. In 2005 the response of representatives of 3 families of egg parasitoids (Mymaridae, Scelionidae, and Trichogrammatidae) to 6 habitat plants (black-eyed Susan, buckwheat, fennel, cock's comb [Celosia cristata L.], Shasta daisy [Leucanthemum × superbum (J. W. Ingram) Berg, ex Kent.], and yarrow [Achillea millefolium L.]) was determined. Crabgrass (Digitaria spp. Haller), that exhibited no obvious flowering, served as a control. Results demonstrated that flower species and height affected insects of all 3 families of parasitoids, but removal of flowers only affected scelionids which were trapped in greater abundance in cock's comb plots at flower height. Mymarids were most abundant at 0.5 times the flower height in black-eyed Susan plots regardless of flower presence, and trichogrammatids were trapped in greatest abundance at 0.5 times the canopy height in unmowed crabgrass plots. Our results indicate that habitat plantings may attract egg parasitoids, but that flowers themselves do not appear to be responsible for this attraction.}, number={4}, journal={JOURNAL OF ENTOMOLOGICAL SCIENCE}, author={Witting, B. E. and Orr, D. B. and Linker, H. M.}, year={2007}, month={Oct}, pages={439–456} }