@misc{gornicki_lambrinow_golkar-narenji_data_domagala_niebora_farzaneh_mozdziak_zabel_antosik_et al._2024, title={Biomimetic Scaffolds-A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering}, volume={14}, ISSN={["2079-4991"]}, url={https://www.mdpi.com/2079-4991/14/6/531}, DOI={10.3390/nano14060531}, abstractNote={Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.}, number={6}, journal={NANOMATERIALS}, author={Gornicki, Tomasz and Lambrinow, Jakub and Golkar-Narenji, Afsaneh and Data, Krzysztof and Domagala, Dominika and Niebora, Julia and Farzaneh, Maryam and Mozdziak, Paul and Zabel, Maciej and Antosik, Pawel and et al.}, year={2024}, month={Mar} }
 @misc{bryl_kulus_bryja_domagala_mozdziak_antosik_bukowska_zabel_dziegiel_kempisty_2024, title={Cardiac progenitor cell therapy: mechanisms of action}, volume={14}, ISSN={["2045-3701"]}, DOI={10.1186/s13578-024-01211-x}, abstractNote={Abstract}, number={1}, journal={CELL AND BIOSCIENCE}, author={Bryl, Rut and Kulus, Magdalena and Bryja, Artur and Domagala, Dominika and Mozdziak, Paul and Antosik, Pawel and Bukowska, Dorota and Zabel, Maciej and Dziegiel, Piotr and Kempisty, Bartosz}, year={2024}, month={Mar} }
 @misc{domagala_data_szyller_farzaneh_mozdziak_wozniak_zabel_dziegiel_kempisty_2024, title={Cellular, Molecular and Clinical Aspects of Aortic Aneurysm-Vascular Physiology and Pathophysiology}, volume={13}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/13/3/274}, DOI={10.3390/cells13030274}, abstractNote={A disturbance of the structure of the aortic wall results in the formation of aortic aneurysm, which is characterized by a significant bulge on the vessel surface that may have consequences, such as distention and finally rupture. Abdominal aortic aneurysm (AAA) is a major pathological condition because it affects approximately 8% of elderly men and 1.5% of elderly women. The pathogenesis of AAA involves multiple interlocking mechanisms, including inflammation, immune cell activation, protein degradation and cellular malalignments. The expression of inflammatory factors, such as cytokines and chemokines, induce the infiltration of inflammatory cells into the wall of the aorta, including macrophages, natural killer cells (NK cells) and T and B lymphocytes. Protein degradation occurs with a high expression not only of matrix metalloproteinases (MMPs) but also of neutrophil gelatinase-associated lipocalin (NGAL), interferon gamma (IFN-γ) and chymases. The loss of extracellular matrix (ECM) due to cell apoptosis and phenotype switching reduces tissue density and may contribute to AAA. It is important to consider the key mechanisms of initiating and promoting AAA to achieve better preventative and therapeutic outcomes.}, number={3}, journal={CELLS}, author={Domagala, Dominika and Data, Krzysztof and Szyller, Hubert and Farzaneh, Maryam and Mozdziak, Paul and Wozniak, Slawomir and Zabel, Maciej and Dziegiel, Piotr and Kempisty, Bartosz}, year={2024}, month={Feb} }
 @article{jeseta_kalina_franzova_fialkova_hosek_mekinova_crha_kempisty_ventruba_navratilova_2024, title={Cross sectional study on exposure to BPA and its analogues and semen parameters in Czech men}, volume={345}, ISSN={["1873-6424"]}, DOI={10.1016/j.envpol.2024.123445}, abstractNote={Exposure to bisphenols has been found to have adverse effects on male reproductive function in animals. Human exposure to bisphenols is widespread. Bisphenol A (BPA) and its analogues, including bisphenol S (BPS), bisphenol F (BPF), and bisphenol AF (BPAF) are utilized in various consumer products such as food contact materials and dental resins. The effects of these compounds on male fertility and spermatogenesis are unclear and findings from human studies are inconsistent. In this cross-sectional study, we evaluated the influence of BPA, BPS, BPF, BPAF (BPs) measured in semen on number of spermatozoa, total motility, progressive motility, morphology, and DNA fragmentation. We also examined the association of bisphenols (BPs) exposure with patients’ occupation. A total of 358 patients aged 17–62 years with BMI 18–42 were included in the study from 2019 to 2021. BPs were extracted using solvent extraction followed by preconcentration step and determined by high-performance liquid chromatography and tandem mass spectrometry (LC/MSMS). Bisphenols were detected in 343 from 349 analysed samples (98.3% of all the samples). In 6 samples, the concentration of all BPs was under the limit of detection and in 20 samples under the limit of quantification. We did not find a statistically significant relationship between occupation and BPs. However, we observed significant correlations between the concentration of BPA and a lower motility and normal morphology. For BPS, a significant correlation with a lower ejaculate volume and a lower total sperm count was found. BPF and BPAF were detected only in 14.3% and 23.9% of samples, respectively. For BPF and BPAF, no significant correlations with spermiogram parameters were observed. Our results show that BPs are widespread in the male population (more than 90% of analysed samples), independently of an occupation and in case of BPA and BPS having a negative impact on spermiogram parameters.}, journal={ENVIRONMENTAL POLLUTION}, author={Jeseta, Michal and Kalina, Jiri and Franzova, Katerina and Fialkova, Sandra and Hosek, Jan and Mekinova, Lenka and Crha, Igor and Kempisty, Bartosz and Ventruba, Pavel and Navratilova, Jana}, year={2024}, month={Mar} }
 @article{kulus_farzaneh_bryja_zehtabi_azizidoost_dari_golcar-narenji_ziemak_chwarzynski_piotrowska-kempisty_et al._2024, title={Phenotypic Transitions the Processes Involved in Regulation of Growth and Proangiogenic Properties of Stem Cells, Cancer Stem Cells and Circulating Tumor Cells}, volume={2}, ISSN={["2629-3277"]}, DOI={10.1007/s12015-024-10691-w}, abstractNote={Abstract}, journal={STEM CELL REVIEWS AND REPORTS}, author={Kulus, Magdalena and Farzaneh, Maryam and Bryja, Artur and Zehtabi, Mojtaba and Azizidoost, Shirin and Dari, Mahrokh Abouali Gale and Golcar-Narenji, Afsaneh and Ziemak, Hanna and Chwarzynski, Mikolaj and Piotrowska-Kempisty, Hanna and et al.}, year={2024}, month={Feb} }
 @article{stefanska_nemcova_blatkiewicz_pienkowski_rucinski_zabel_mozdziak_podhorska-okolow_dziegiel_kempisty_2023, title={Apoptosis Related Human Wharton's Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells-Complete Transcriptomic Assays}, volume={24}, ISSN={["1422-0067"]}, url={https://www.mdpi.com/1422-0067/24/12/10023}, DOI={10.3390/ijms241210023}, abstractNote={Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) exhibit multilineage differentiation potential, adhere to plastic, and express a specific set of surface markers—CD105, CD73, CD90. Although there are relatively well-established differentiation protocols for WJ-MSCs, the exact molecular mechanisms involved in their in vitro long-term culture and differentiation remain to be elucidated. In this study, the cells were isolated from Wharton’s jelly of umbilical cords obtained from healthy full-term deliveries, cultivated in vitro, and differentiated towards osteogenic, chondrogenic, adipogenic and neurogenic lineages. RNA samples were isolated after the differentiation regimen and analyzed using an RNA sequencing (RNAseq) assay, which led to the identification of differentially expressed genes belonging to apoptosis-related ontological groups. ZBTB16 and FOXO1 were upregulated in all differentiated groups as compared to controls, while TGFA was downregulated in all groups. In addition, several possible novel marker genes associated with the differentiation of WJ-MSCs were identified (e.g., SEPTIN4, ITPR1, CNR1, BEX2, CD14, EDNRB). The results of this study provide an insight into the molecular mechanisms involved in the long-term culture in vitro and four-lineage differentiation of WJ-MSCs, which is crucial to utilize WJ-MSCs in regenerative medicine.}, number={12}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Stefanska, Katarzyna and Nemcova, Lucie and Blatkiewicz, Malgorzata and Pienkowski, Wojciech and Rucinski, Marcin and Zabel, Maciej and Mozdziak, Paul and Podhorska-Okolow, Marzenna and Dziegiel, Piotr and Kempisty, Bartosz}, year={2023}, month={Jun} }
 @misc{kulus_jankowski_kranc_narenji_farzaneh_dziegiel_zabel_antosik_bukowska_mozdziak_et al._2023, title={Bioreactors, scaffolds and microcarriers and in vitro meat production-current obstacles and potential solutions}, volume={10}, ISSN={["2296-861X"]}, DOI={10.3389/fnut.2023.1225233}, abstractNote={In vitro meat production presents a potential viable alternative for meat consumption, which could provide the consumer with a product indistinguishable from the original, with very similar nutritional and culinary values. Indeed, the alternative products currently accessible often lack comparable nutritional value or culinary attributes to their animal-derived counterparts. This creates challenges for their global acceptance, particularly in countries where meat consumption holds cultural significance. However, while cultured meat research has been progressing rapidly in recent years, some significant obstacles still need to be overcome before its possible commercialization. Hence, this review summarizes the most current knowledge regarding the history of cultured meat, the currently used cell sources and methods used for the purpose of in vitro meat production, with particular focus on the role of bioreactors, scaffolds and microcarriers in overcoming the current obstacles. The authors put the potential microcarrier and scaffold-based solutions in a context, discussing the ways in which they can impact the way forward for the technology, including the use of considering the potential practical and societal barriers to implementing it as a viable food source worldwide.}, journal={FRONTIERS IN NUTRITION}, author={Kulus, Magdalena and Jankowski, Maurycy and Kranc, Wieslawa and Narenji, Afsaneh Golkar and Farzaneh, Maryam and Dziegiel, Piotr and Zabel, Maciej and Antosik, Pawel and Bukowska, Dorota and Mozdziak, Paul and et al.}, year={2023}, month={Sep} }
 @article{ghaedrahmati_nasrolahi_najafi_mighani_anbiyaee_haybar_assareh_kempisty_dziegiel_azizidoost_et al._2023, title={Circular RNAs-mediated angiogenesis in human cancers}, ISSN={["1699-3055"]}, DOI={10.1007/s12094-023-03178-6}, abstractNote={Circular RNAs (circRNAs) as small non-coding RNAs with cell, tissue, or organ-specific expression accomplish a broad array of functions in physiological and pathological processes such as cancer development. Angiogenesis, a complicated multistep process driving a formation of new blood vessels, speeds up tumor progression by supplying nutrients as well as energy. Abnormal expression of circRNAs reported to affect tumor development through impressing angiogenesis. Such impacts are introduced as constant with different tumorigenic features known as "hallmarks of cancer". In addition, deregulated circRNAs show possibilities to prognosis and diagnosis both in the prophecy of prognosis in malignancies and also their prejudice from healthy individuals. In the present review article, we have evaluated the angiogenic impacts and anti-angiogenic managements of circRNAs in human cancers.}, journal={CLINICAL & TRANSLATIONAL ONCOLOGY}, author={Ghaedrahmati, Farhoodeh and Nasrolahi, Ava and Najafi, Sajad and Mighani, Mohammad and Anbiyaee, Omid and Haybar, Habib and Assareh, Ahmad Reza and Kempisty, Bartosz and Dziegiel, Piotr and Azizidoost, Shirin and et al.}, year={2023}, month={Apr} }
 @misc{data_kulus_ziemak_chwarzynski_piotrowska-kempisty_bukowska_antosik_mozdziak_kempisty_2023, title={Decellularization of Dense Regular Connective Tissue-Cellular and Molecular Modification with Applications in Regenerative Medicine}, volume={12}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/12/18/2293}, DOI={10.3390/cells12182293}, abstractNote={Healing of dense regular connective tissue, due to a high fiber-to-cell ratio and low metabolic activity and regeneration potential, frequently requires surgical implantation or reconstruction with high risk of reinjury. An alternative to synthetic implants is using bioscaffolds obtained through decellularization, a process where the aim is to extract cells from the tissue while preserving the tissue-specific native molecular structure of the ECM. Proteins, lipids, nucleic acids and other various extracellular molecules are largely involved in differentiation, proliferation, vascularization and collagen fibers deposit, making them the crucial processes in tissue regeneration. Because of the multiple possible forms of cell extraction, there is no standardized protocol in dense regular connective tissue (DRCT). Many modifications of the structure, shape and composition of the bioscaffold have also been described to improve the therapeutic result following the implantation of decellularized connective tissue. The available data provide a valuable source of crucial information. However, the wide spectrum of decellularization makes it important to understand the key aspects of bioscaffolds relative to their potential use in tissue regeneration.}, number={18}, journal={CELLS}, author={Data, Krzysztof and Kulus, Magdalena and Ziemak, Hanna and Chwarzynski, Mikolaj and Piotrowska-Kempisty, Hanna and Bukowska, Dorota and Antosik, Pawel and Mozdziak, Paul and Kempisty, Bartosz}, year={2023}, month={Sep} }
 @misc{issa_jaber_rifai_mozdziak_kempisty_dyszkiewicz-konwinska_2023, title={Diagnostic Test Accuracy of Artificial Intelligence in Detecting Periapical Periodontitis on Two-Dimensional Radiographs: A Retrospective Study and Literature Review}, volume={59}, ISSN={["1648-9144"]}, url={https://www.mdpi.com/1648-9144/59/4/768}, DOI={10.3390/medicina59040768}, abstractNote={This study aims to evaluate the diagnostic accuracy of artificial intelligence in detecting apical pathosis on periapical radiographs. A total of twenty anonymized periapical radiographs were retrieved from the database of Poznan University of Medical Sciences. These radiographs displayed a sequence of 60 visible teeth. The evaluation of the radiographs was conducted using two methods (manual and automatic), and the results obtained from each technique were afterward compared. For the ground-truth method, one oral and maxillofacial radiology expert with more than ten years of experience and one trainee in oral and maxillofacial radiology evaluated the radiographs by classifying teeth as healthy and unhealthy. A tooth was considered unhealthy when periapical periodontitis related to this tooth had been detected on the radiograph. At the same time, a tooth was classified as healthy when no periapical radiolucency was detected on the periapical radiographs. Then, the same radiographs were evaluated by artificial intelligence, Diagnocat (Diagnocat Ltd., San Francisco, CA, USA). Diagnocat (Diagnocat Ltd., San Francisco, CA, USA) correctly identified periapical lesions on periapical radiographs with a sensitivity of 92.30% and identified healthy teeth with a specificity of 97.87%. The recorded accuracy and F1 score were 96.66% and 0.92, respectively. The artificial intelligence algorithm misdiagnosed one unhealthy tooth (false negative) and over-diagnosed one healthy tooth (false positive) compared to the ground-truth results. Diagnocat (Diagnocat Ltd., San Francisco, CA, USA) showed an optimum accuracy for detecting periapical periodontitis on periapical radiographs. However, more research is needed to assess the diagnostic accuracy of artificial intelligence-based algorithms in dentistry.}, number={4}, journal={MEDICINA-LITHUANIA}, author={Issa, Julien and Jaber, Mouna and Rifai, Ismail and Mozdziak, Paul and Kempisty, Bartosz and Dyszkiewicz-Konwinska, Marta}, year={2023}, month={Apr} }
 @article{sheykhi-sabzehpoush_ghasemian_pour_mighani_moghanibashi_jafari_zabel_dziegiel_farzaneh_kempisty_2023, title={Emerging roles of long non-coding RNA FTX in human disorders}, ISSN={["1699-3055"]}, DOI={10.1007/s12094-023-03163-z}, abstractNote={Long non-coding RNAs (lncRNAs) are involved the progression of cancerous and non-cancerous disorders via different mechanism. FTX (five prime to xist) is an evolutionarily conserved lncRNA that is located upstream of XIST and regulates its expression. FTX participates in progression of various malignancy including gastric cancer, glioma, ovarian cancer, pancreatic cancer, and retinoblastoma. Also, FTX can be involved in the pathogenesis of non-cancerous disorders such as endometriosis and stroke. FTX acts as competitive endogenous RNA (ceRNA) and via sponging various miRNAs, including miR-186, miR-200a-3p, miR-215-3p, and miR-153-3p to regulate the expression of their downstream target. FTX by targeting various signaling pathways including Wnt/β-catenin, PI3K/Akt, SOX4, PDK1/PKB/GSK-3β, TGF-β1, FOXA2, and PPARγ regulate molecular mechanism involved in various disorders. Dysregulation of FTX is associated with an increased risk of various disorders. Therefore, FTX and its downstream targets may be suitable biomarkers for the diagnosis and treatment of human malignancies. In this review, we summarized the emerging roles of FTX in human cancerous and non-cancerous cells.}, journal={CLINICAL & TRANSLATIONAL ONCOLOGY}, author={Sheykhi-Sabzehpoush, Mohadeseh and Ghasemian, Majid and Pour, Fatemeh Khojasteh and Mighani, Mohammad and Moghanibashi, Mehdi and Jafari, Razieh Mohammad and Zabel, Maciej and Dziegiel, Piotr and Farzaneh, Maryam and Kempisty, Bartosz}, year={2023}, month={Apr} }
 @misc{jozkowiak_piotrowska-kempisty_kobylarek_gorska_mozdziak_kempisty_rachon_spaczynski_2023, title={Endocrine Disrupting Chemicals in Polycystic Ovary Syndrome: The Relevant Role of the Theca and Granulosa Cells in the Pathogenesis of the Ovarian Dysfunction}, volume={12}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/12/1/174}, DOI={10.3390/cells12010174}, abstractNote={Polycystic ovary syndrome (PCOS) is the most common heterogeneous endocrine disorder among women of reproductive age. The pathogenesis of PCOS remains elusive; however, there is evidence suggesting the potential contribution of genetic interactions or predispositions combined with environmental factors. Among these, endocrine disrupting chemicals (EDCs) have been proposed to potentially contribute to the etiology of PCOS. Granulosa and theca cells are known to cooperate to maintain ovarian function, and any disturbance can lead to endocrine disorders, such as PCOS. This article provides a review of the recent knowledge on PCOS pathophysiology, the role of granulosa and theca cells in PCOS pathogenesis, and the evidence linking exposure to EDCs with reproductive disorders such as PCOS.}, number={1}, journal={CELLS}, author={Jozkowiak, Malgorzata and Piotrowska-Kempisty, Hanna and Kobylarek, Dominik and Gorska, Natalia and Mozdziak, Paul and Kempisty, Bartosz and Rachon, Dominik and Spaczynski, Robert Z.}, year={2023}, month={Jan} }
 @article{stefanska_nemcova_blatkiewicz_zok_kaczmarek_pienkowski_mozdziak_piotrowska-kempisty_kempisty_2023, title={Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton's Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells}, volume={24}, ISSN={["1422-0067"]}, url={https://www.mdpi.com/1422-0067/24/16/12939}, DOI={10.3390/ijms241612939}, abstractNote={Wharton’s jelly (WJ) contains mesenchymal stem cells (MSCs) exhibiting broad immunomodulatory properties and differentiation capacity, which makes them a promising tool for cellular therapies. Although the osteogenic, chondrogenic and adipogenic differentiation is a gold standard for proper identification of MSCs, it is important to elucidate the exact molecular mechanisms governing these processes to develop safe and efficient cellular therapies. Umbilical cords were collected from healthy, full-term deliveries, for subsequent MSCs (WJ-MSCs) isolation. WJ-MSCs were cultivated in vitro for osteogenic, chondrogenic, adipogenic and neurogenic differentiation. The RNA samples were isolated and the transcript levels were evaluated using NovaSeq platform, which led to the identification of differentially expressed genes. Expression of H19 and SLPI was enhanced in adipocytes, chondrocytes and osteoblasts, and NPPB was decreased in all analyzed groups compared to the control. KISS1 was down-regulated in adipocytes, chondrocytes, and neural-like cells compared to the control. The most of identified genes were already implicated in differentiation of MSCs; however, some genes (PROK1, OCA2) have not yet been associated with initiating final cell fate. The current results indicate that both osteo- and adipo-induced WJ-MSCs share many similarities regarding the most overexpressed genes, while the neuro-induced WJ-MSCs are quite distinctive from the other three groups. Overall, this study provides an insight into the transcriptomic changes occurring during the differentiation of WJ-MSCs and enables the identification of novel markers involved in this process, which may serve as a reference for further research exploring the role of these genes in physiology of WJ-MSCs and in regenerative medicine.}, number={16}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Stefanska, Katarzyna and Nemcova, Lucie and Blatkiewicz, Malgorzata and Zok, Agnieszka and Kaczmarek, Mariusz and Pienkowski, Wojciech and Mozdziak, Paul and Piotrowska-Kempisty, Hanna and Kempisty, Bartosz}, year={2023}, month={Aug} }
 @article{kulus_kranc_kulus_dziegiel_bukowska_mozdziak_kempisty_antosik_2023, title={Expression of genes regulating cell division in porcine follicular granulosa cells}, volume={18}, ISSN={["1747-1028"]}, DOI={10.1186/s13008-023-00094-7}, abstractNote={Abstract}, number={1}, journal={CELL DIVISION}, author={Kulus, Jakub and Kranc, Wieslawa and Kulus, Magdalena and Dziegiel, Piotr and Bukowska, Dorota and Mozdziak, Paul and Kempisty, Bartosz and Antosik, Pawel}, year={2023}, month={Aug} }
 @article{golkar-narenji_dziegiel_kempisty_petitte_mozdziak_bryja_2023, title={In vitro culture of reptile PGCS to preserve endangered species}, volume={5}, ISSN={["1095-8355"]}, DOI={10.1002/cbin.12033}, abstractNote={Abstract}, journal={CELL BIOLOGY INTERNATIONAL}, author={Golkar-Narenji, Afsaneh and Dziegiel, Piotr and Kempisty, Bartosz and Petitte, James and Mozdziak, Paul Edward and Bryja, Artur}, year={2023}, month={May} }
 @article{kulus_kranc_kulus_bukowska_piotrowska-kempisty_mozdziak_kempisty_antosik_2023, title={New Gene Markers of Exosomal Regulation Are Involved in Porcine Granulosa Cell Adhesion, Migration, and Proliferation}, volume={24}, ISSN={["1422-0067"]}, url={https://www.mdpi.com/1422-0067/24/14/11873}, DOI={10.3390/ijms241411873}, abstractNote={Exosomal regulation is intimately involved in key cellular processes, such as migration, proliferation, and adhesion. By participating in the regulation of basic mechanisms, extracellular vesicles are important in intercellular signaling and the functioning of the mammalian reproductive system. The complexity of intercellular interactions in the ovarian follicle is also based on multilevel intercellular signaling, including the mechanisms involving cadherins, integrins, and the extracellular matrix. The processes in the ovary leading to the formation of a fertilization-ready oocyte are extremely complex at the molecular level and depend on the oocyte’s ongoing relationship with granulosa cells. An analysis of gene expression from material obtained from a primary in vitro culture of porcine granulosa cells was employed using microarray technology. Genes with the highest expression (LIPG, HSD3B1, CLIP4, LOX, ANKRD1, FMOD, SHAS2, TAGLN, ITGA8, MXRA5, and NEXN) and the lowest expression levels (DAPL1, HSD17B1, SNX31, FST, NEBL, CXCL10, RGS2, MAL2, IHH, and TRIB2) were selected for further analysis. The gene expression results obtained from the microarrays were validated using quantitative RT-qPCR. Exosomes may play important roles regarding intercellular signaling between granulosa cells. Therefore, exosomes may have significant applications in regenerative medicine, targeted therapy, and assisted reproduction technologies.}, number={14}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Kulus, Jakub and Kranc, Wieslawa and Kulus, Magdalena and Bukowska, Dorota and Piotrowska-Kempisty, Hanna and Mozdziak, Paul and Kempisty, Bartosz and Antosik, Pawel}, year={2023}, month={Jul} }
 @misc{jeseta_pospisilova_mekinova_franzova_ventruba_lousova_kempisty_ozdian_zakova_crha_2023, title={Non-Invasive Diagnostics of Male Spermatogenesis from Seminal Plasma: Seminal Proteins}, volume={13}, ISSN={["2075-4418"]}, DOI={10.3390/diagnostics13152468}, abstractNote={The compounds of seminal plasma have great potential as biomarkers of male fertility and can be used as a diagnostic tool for types of azoospermia. Azoospermia occurs in approximately 1% of the male population, and for an effective therapy of this form of male infertility, it is important to distinguish between obstructive and non-obstructive azoospermia. Proteins in seminal plasma can serve as biomarkers for diagnosing azoospermia. Considering the various types of obstructions, a combination of multiple proteins is advisable for diagnostic purposes. In this context, testicular and epididymal proteins are particularly significant, as they are specific to these tissues and typically absent in ejaculate during most obstructions. A combination of multiple biomarkers is more effective than the analysis of a single protein. This group of markers contains TEX101 and ECM1 proteins, combined detections of these two bring a diagnostic output with a high sensitivity and specificity. Similar results were observed for combined detection of TEX101 and SPAG1. The effective using of specific biomarkers from seminal plasma can significantly improve the existing approaches to diagnosis of the causes of male infertility.}, number={15}, journal={DIAGNOSTICS}, author={Jeseta, Michal and Pospisilova, Anna and Mekinova, Lenka and Franzova, Katerina and Ventruba, Pavel and Lousova, Eva and Kempisty, Bartosz and Ozdian, Tomas and Zakova, Jana and Crha, Igor}, year={2023}, month={Aug} }
 @misc{azizidoost_nasrolahi_sheykhi-sabzehpoush_akiash_assareh_anbiyaee_antosik_dzi_farzaneh_kempisty_2023, title={Potential roles of endothelial cells-related non-coding RNAs in cardiovascular diseases}, volume={242}, ISSN={["1618-0631"]}, DOI={10.1016/j.prp.2023.154330}, abstractNote={Endothelial dysfunction is identified by a conversion of the endothelium toward decreased vasodilation and prothrombic features and is known as a primary pathogenic incident in cardiovascular diseases. An insight based on particular and promising biomarkers of endothelial dysfunction may possess vital clinical significances. Currently, non-coding RNAs due to their participation in critical cardiovascular processes like initiation and progression have gained much attention as possible diagnostic as well as prognostic biomarkers in cardiovascular diseases. Emerging line of proof has demonstrated that abnormal expression of non-coding RNAs is nearly correlated with the pathogenesis of cardiovascular diseases. In the present review, we focus on the expression and functional effects of various kinds of non-coding RNAs in cardiovascular diseases and negotiate their possible clinical implications as diagnostic or prognostic biomarkers and curative targets.}, journal={PATHOLOGY RESEARCH AND PRACTICE}, author={Azizidoost, Shirin and Nasrolahi, Ava and Sheykhi-Sabzehpoush, Mohadeseh and Akiash, Nehzat and Assareh, Ahmad Reza and Anbiyaee, Omid and Antosik, Pawel and Dzi, Piotr and Farzaneh, Maryam and Kempisty, Bartosz}, year={2023}, month={Feb} }
 @article{nasrolahi_pour_salehi_kempisty_hajizadeh_feghhi_azizidoost_farzaneh_2023, title={Potential roles of lncRNA MALAT1-miRNA interactions in ocular diseases}, ISSN={["1873-961X"]}, DOI={10.1007/s12079-023-00787-2}, journal={JOURNAL OF CELL COMMUNICATION AND SIGNALING}, author={Nasrolahi, Ava and Pour, Fatemeh Khojasteh and Salehi, Abdolah Mousavi and Kempisty, Bartosz and Hajizadeh, Maryam and Feghhi, Mostafa and Azizidoost, Shirin and Farzaneh, Maryam}, year={2023}, month={Oct} }
 @misc{bryja_zadka_farzaneh_zehtabi_ghasemian_dyszkiewicz-konwinska_mozdziak_zabel_podhorska-okolow_dziegiel_et al._2023, title={Small extracellular vesicles - A host for advanced bioengineering and "Trojan Horse" of non-coding RNAs}, volume={332}, ISSN={["1879-0631"]}, DOI={10.1016/j.lfs.2023.122126}, abstractNote={Small extracellular vesicles (sEVs) are a type of membranous vesicles that can be released by cells into the extracellular space. The relationship between sEVs and non-coding RNAs (ncRNAs) is highly intricate and interdependent. This symbiotic relationship plays a pivotal role in facilitating intercellular communication and holds profound implications for a myriad of biological processes. The concept of sEVs and their ncRNA cargo as a "Trojan Horse" highlights their remarkable capacity to traverse biological barriers and surreptitiously deliver their cargo to target cells, evading detection by the host-immune system. Accumulating evidence suggests that sEVs may be harnessed as carriers to ferry therapeutic ncRNAs capable of selectively silencing disease-driving genes, particularly in conditions such as cancer. This approach presents several advantages over conventional drug delivery methods, opening up new possibilities for targeted therapy and improved treatment outcomes. However, the utilization of sEVs and ncRNAs as therapeutic agents raises valid concerns regarding the possibility of unforeseen consequences and unintended impacts that may emerge from their application. It is important to consider the fundamental attributes of sEVs and ncRNAs, including by an in-depth analysis of the practical and clinical potentials of exosomes, serving as a representative model for sEVs encapsulating ncRNAs.}, journal={LIFE SCIENCES}, author={Bryja, Artur and Zadka, Lukasz and Farzaneh, Maryam and Zehtabi, Mojtaba and Ghasemian, Majid and Dyszkiewicz-Konwinska, Marta and Mozdziak, Paul and Zabel, Maciej and Podhorska-Okolow, Marzenna and Dziegiel, Piotr and et al.}, year={2023}, month={Nov} }
 @article{jozkowiak_kobylarek_bryja_gogola-mruk_czajkowski_skupin-mrugalska_kempisty_spaczynski_piotrowska-kempisty_2023, title={Steroidogenic activity of liposomal methylated resveratrol analog 3,4,5,4 & PRIME;-tetramethoxystilbene (DMU-212) in human luteinized granulosa cells in a primary three-dimensional in vitro model}, ISSN={["1559-0100"]}, DOI={10.1007/s12020-023-03458-9}, abstractNote={Abstract}, journal={ENDOCRINE}, author={Jozkowiak, Malgorzata and Kobylarek, Dominik and Bryja, Artur and Gogola-Mruk, Justyna and Czajkowski, Mikolaj and Skupin-Mrugalska, Paulina and Kempisty, Bartosz and Spaczynski, Robert Z. and Piotrowska-Kempisty, Hanna}, year={2023}, month={Aug} }
 @article{farzaneh_anbiyaee_azizidoost_nasrolahi_ghaedrahmati_kempisty_mozdziak_khoshnam_najafi_2023, title={The Mechanisms of Long Non-coding RNA-XIST in Ischemic Stroke: Insights into Functional Roles and Therapeutic Potential}, volume={11}, ISSN={["1559-1182"]}, DOI={10.1007/s12035-023-03740-x}, journal={MOLECULAR NEUROBIOLOGY}, author={Farzaneh, Maryam and Anbiyaee, Omid and Azizidoost, Shirin and Nasrolahi, Ava and Ghaedrahmati, Farhoodeh and Kempisty, Bartosz and Mozdziak, Paul and Khoshnam, Seyed Esmaeil and Najafi, Sajad}, year={2023}, month={Nov} }
 @misc{stefanska_jozkowiak_angelova volponi_shibli_golkar-narenji_antosik_bukowska_piotrowska-kempisty_mozdziak_dziegiel_et al._2023, title={The Role of Exosomes in Human Carcinogenesis and Cancer Therapy-Recent Findings from Molecular and Clinical Research}, volume={12}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/12/3/356}, DOI={10.3390/cells12030356}, abstractNote={Exosomes are biological nanoscale spherical lipid bilayer vesicles, 40–160 nm in diameter, produced by most mammalian cells in both physiological and pathological conditions. Exosomes are formed via the endosomal sorting complex required for transport (ESCRT). The primary function of exosomes is mediating cell-to-cell communication. In terms of cancer, exosomes play important roles as mediators of intercellular communication, leading to tumor progression. Moreover, they can serve as biomarkers for cancer detection and progression. Therefore, their utilization in cancer therapies has been suggested, either as drug delivery carriers or as a diagnostic tool. However, exosomes were also reported to be involved in cancer drug resistance via transferring information of drug resistance to sensitive cells. It is important to consider the current knowledge regarding the role of exosomes in cancer, drug resistance, cancer therapies, and their clinical application in cancer therapies.}, number={3}, journal={CELLS}, author={Stefanska, Katarzyna and Jozkowiak, Malgorzata and Angelova Volponi, Ana and Shibli, Jamil Awad and Golkar-Narenji, Afsaneh and Antosik, Pawel and Bukowska, Dorota and Piotrowska-Kempisty, Hanna and Mozdziak, Paul and Dziegiel, Piotr and et al.}, year={2023}, month={Feb} }
 @misc{anbiyaee_moalemnia_ghaedrahmati_shooshtari_khoshnam_kempisty_halili_farzaneh_morenikeji_2023, title={The functions of long non-coding RNA (lncRNA)-MALAT-1 in the pathogenesis of renal cell carcinoma}, volume={24}, ISSN={["1471-2369"]}, DOI={10.1186/s12882-023-03438-1}, abstractNote={Abstract}, number={1}, journal={BMC NEPHROLOGY}, author={Anbiyaee, Omid and Moalemnia, Arash and Ghaedrahmati, Farhoodeh and Shooshtari, Maryam Khombi and Khoshnam, Seyed Esmaeil and Kempisty, Bartosz and Halili, Shahla Ahmadi and Farzaneh, Maryam and Morenikeji, Olanrewaju B.}, year={2023}, month={Dec} }
 @misc{jankowski_farzaneh_ghaedrahmati_shirvaliloo_moalemnia_kulus_ziemak_chwarzynski_dziegiel_zabel_et al._2023, title={Unveiling Mesenchymal Stem Cells' Regenerative Potential in Clinical Applications: Insights in miRNA and lncRNA Implications}, volume={12}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/12/21/2559}, DOI={10.3390/cells12212559}, abstractNote={It is now widely recognized that mesenchymal stem cells (MSCs) possess the capacity to differentiate into a wide array of cell types. Numerous studies have identified the role of lncRNA in the regulation of MSC differentiation. It is important to elucidate the role and interplay of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the regulation of signalling pathways that govern MSC function. Furthermore, miRNAs and lncRNAs are important clinical for innovative strategies aimed at addressing a wide spectrum of existing and emerging disease. Hence it is important to consider their impact on MSC function and differentiation. Examining the data available in public databases, we have collected the literature containing the latest discoveries pertaining to human stem cells and their potential in both fundamental research and clinical applications. Furthermore, we have compiled completed clinical studies that revolve around the application of MSCs, shedding light on the opportunities presented by harnessing the regulatory potential of miRNAs and lncRNAs. This exploration of the therapeutic possibilities offered by miRNAs and lncRNAs within MSCs unveils exciting prospects for the development of precision therapies and personalized treatment approaches. Ultimately, these advancements promise to augment the efficacy of regenerative strategies and produce positive outcomes for patients. As research in this field continues to evolve, it is imperative to explore and exploit the vast potential of miRNAs and lncRNAs as therapeutic agents. The findings provide a solid basis for ongoing investigations, fuelling the quest to fully unlock the regenerative potential of MSCs.}, number={21}, journal={CELLS}, author={Jankowski, Maurycy and Farzaneh, Maryam and Ghaedrahmati, Farhoodeh and Shirvaliloo, Milad and Moalemnia, Arash and Kulus, Magdalena and Ziemak, Hanna and Chwarzynski, Mikolaj and Dziegiel, Piotr and Zabel, Maciej and et al.}, year={2023}, month={Nov} }
 @misc{issa_abou chaar_kempisty_gasiorowski_olszewski_mozdziak_dyszkiewicz-konwinska_2022, title={Artificial-Intelligence-Based Imaging Analysis of Stem Cells: A Systematic Scoping Review}, volume={11}, ISSN={["2079-7737"]}, url={https://www.mdpi.com/2079-7737/11/10/1412}, DOI={10.3390/biology11101412}, abstractNote={This systematic scoping review aims to map and identify the available artificial-intelligence-based techniques for imaging analysis, the characterization of stem cell differentiation, and trans-differentiation pathways. On the ninth of March 2022, data were collected from five electronic databases (PubMed, Medline, Web of Science, Cochrane, and Scopus) and manual citation searching; all data were gathered in Zotero 5.0. A total of 4422 articles were collected after deduplication; only twenty-seven studies were included in this systematic scoping review after a two-phase screening against inclusion criteria by two independent reviewers. The amount of research in this field is significantly increasing over the years. While the current state of artificial intelligence (AI) can tackle a multitude of medical problems, the consensus amongst researchers remains that AI still falls short in multiple ways that investigators should examine, ranging from the quality of images used in training sets and appropriate sample size, as well as the unexpected events that may occur which the algorithm cannot predict.}, number={10}, journal={BIOLOGY-BASEL}, author={Issa, Julien and Abou Chaar, Mazen and Kempisty, Bartosz and Gasiorowski, Lukasz and Olszewski, Raphael and Mozdziak, Paul and Dyszkiewicz-Konwinska, Marta}, year={2022}, month={Oct} }
 @misc{bryl_piwocka_kawka_mozdziak_kempisty_knopik-skrocka_2022, title={Cancer Stem Cells-The Insight into Non-Coding RNAs}, volume={11}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/11/22/3699}, DOI={10.3390/cells11223699}, abstractNote={Since their initial identification three decades ago, there has been extensive research regarding cancer stem cells (CSCs). It is important to consider the biology of cancer stem cells with a particular focus on their phenotypic and metabolic plasticity, the most important signaling pathways, and non-coding RNAs (ncRNAs) regulating these cellular entities. Furthermore, the current status of therapeutic approaches against CSCs is an important consideration regarding employing the technology to improve human health. Cancer stem cells have claimed to be one of the most important group of cells for the development of several common cancers as they dictate features, such as resistance to radio- and chemotherapy, metastasis, and secondary tumor formation. Therapies which could target these cells may develop into an effective strategy for tumor eradication and a hope for patients for whom this disease remains uncurable.}, number={22}, journal={CELLS}, author={Bryl, Rut and Piwocka, Oliwia and Kawka, Emilia and Mozdziak, Paul and Kempisty, Bartosz and Knopik-Skrocka, Agnieszka}, year={2022}, month={Nov} }
 @article{chermula_kranc_celichowski_stelmach_piotrowska-kempisty_mozdziak_pawelczyk_spaczynski_kempisty_2022, title={Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers-Clinical Approach}, volume={11}, ISSN={["2077-0383"]}, url={https://www.mdpi.com/2077-0383/11/1/73}, DOI={10.3390/jcm11010073}, abstractNote={In the growing ovarian follicle, the maturing oocyte is accompanied by cumulus (CCs) and granulosa (GCs) cells. Currently, there remain many unanswered questions about the epithelial origin of these cells. Global and targeted gene transcript levels were assessed on 1, 7, 15, 30 days of culture for CCs and GCs. Detailed analysis of the genes belonging to epithelial cell-associated ontological groups allowed us to assess a total of 168 genes expressed in CCs (97 genes) and GCs (71 genes) during long-term in vitro culture. Expression changes of the analyzed genes allowed the identification of the group of genes: TGFBR3, PTGS2, PRKX, AHI1, and IL11, whose expression decreased the most and the group of ANXA3, DKK1, CCND1, STC1, CAV1, and SFRP4 genes, whose expression significantly increased. These genes’ expression indicates CCs and GCs epithelialization processes and their epithelial origin. Expression change analysis of genes involved in epithelization processes in GCs and CCs during their in vitro culture made it possible to describe the most significantly altered of the 11 genes. Detailed analysis of gene expression in these two cell populations at different time intervals confirms their ovarian surface epithelial origin. Furthermore, some gene expression profiles appear to have tumorigenic properties, suggesting that granulosa cells may play a role in cancerogenesis.}, number={1}, journal={JOURNAL OF CLINICAL MEDICINE}, author={Chermula, Blazej and Kranc, Wieslawa and Celichowski, Piotr and Stelmach, Boguslawa and Piotrowska-Kempisty, Hanna and Mozdziak, Paul and Pawelczyk, Leszek and Spaczynski, Robert Zygmunt and Kempisty, Bartosz}, year={2022}, month={Jan} }
 @article{jankowski_kaczmarek_wasiatycz_konwerska_dompe_bukowska_antosik_mozdziak_kempisty_2022, title={Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes}, volume={13}, ISSN={["2073-4425"]}, url={https://www.mdpi.com/2073-4425/13/9/1664}, DOI={10.3390/genes13091664}, abstractNote={The interest in stem cell research continuously increased over the last decades, becoming one of the most important trends in the 21st century medicine. Stem cell-based therapies have a potential to become a solution for a range of currently untreatable diseases, such as spinal cord injuries, type I diabetes, Parkinson’s disease, heart disease, stroke, and osteoarthritis. Hence, this study, based on canine material, aims to investigate the molecular basis of adipose-derived stem cell (ASC) differentiation into chondrocytes, to serve as a transcriptomic reference for further research aiming to introduce ASC into treatment of bone and cartilage related diseases, such as osteoarthritis in veterinary medicine. Adipose tissue samples were harvested from a canine specimen subjected to a routine ovariohysterecromy procedure at an associated veterinary clinic. The material was treated for ASC isolation and chondrogenic differentiation. RNA samples were isolated at day 1 of culture, day 30 of culture in unsupplemented culture media, and day 30 of culture in chondrogenic differentiation media. The resulting RNA was analyzed using RNAseq assays, with the results validated by RT-qPCR. Between differentiated chondrocytes, early and late cultures, most up- and down-regulated genes in each comparison were selected for further analysis., there are several genes (e.g., MMP12, MPEG1, CHI3L1, and CD36) that could be identified as new markers of chondrogenesis and the influence of long-term culture conditions on ASCs. The results of the study prove the usefulness of the in vitro culture model, providing further molecular insight into the processes associated with ASC culture and differentiation. Furthermore, the knowledge obtained could be used as a molecular reference for future in vivo and clinical studies.}, number={9}, journal={GENES}, author={Jankowski, Maurycy and Kaczmarek, Mariusz and Wasiatycz, Grzegorz and Konwerska, Aneta and Dompe, Claudia and Bukowska, Dorota and Antosik, Pawel and Mozdziak, Paul and Kempisty, Bartosz}, year={2022}, month={Sep} }
 @misc{sibiak_ozegowska_wender-ozegowska_gutaj_mozdziak_kempisty_2022, title={Fetomaternal Expression of Glucose Transporters (GLUTs)-Biochemical, Cellular and Clinical Aspects}, volume={14}, ISSN={["2072-6643"]}, url={https://www.mdpi.com/2072-6643/14/10/2025}, DOI={10.3390/nu14102025}, abstractNote={Several types of specialized glucose transporters (GLUTs) provide constant glucose transport from the maternal circulation to the developing fetus through the placental barrier from the early stages of pregnancy. GLUT1 is a prominent protein isoform that regulates placental glucose transfer via glucose-facilitated diffusion. The GLUT1 membrane protein density and permeability of the syncytial basal membrane (BM) are the main factors limiting the rate of glucose diffusion in the fetomaternal compartment in physiological conditions. Besides GLUT1, the GLUT3 and GLUT4 isoforms are widely expressed across the human placenta. Numerous medical conditions and molecules, such as hormones, adipokines, and xenobiotics, alter the GLUT’s mRNA and protein expression. Diabetes upregulates the BM GLUT’s density and promotes fetomaternal glucose transport, leading to excessive fetal growth. However, most studies have found no between-group differences in GLUTs’ placental expression in macrosomic and normal control pregnancies. The fetomaternal GLUTs expression may also be influenced by several other conditions, such as chronic hypoxia, preeclampsia, and intrahepatic cholestasis of pregnancy.}, number={10}, journal={NUTRIENTS}, author={Sibiak, Rafal and Ozegowska, Katarzyna and Wender-Ozegowska, Ewa and Gutaj, Pawel and Mozdziak, Paul and Kempisty, Bartosz}, year={2022}, month={May} }
 @article{jeseta_franzova_machynova_kalina_kohoutek_mekinova_crha_kempisty_kasik_zakova_et al._2022, title={The Bisphenols Found in the Ejaculate of Men Does Not Pass through the Testes}, volume={10}, ISSN={["2305-6304"]}, DOI={10.3390/toxics10060311}, abstractNote={Exposure to bisphenols is related to negative effects on male reproduction. The bisphenols exposure is associated with several modes of action including negative impact on the blood–testis barrier (BTB) in testes or direct effect on spermatozoa. Bisphenols have been detected in human seminal plasma, but the possible mechanism of seminal transfer of bisphenols is not clear. Some authors consider the transfer through the blood–testis barrier to be crucial. Therefore, in this work, we compared normozoospermic men and men after vasectomy who have interrupted vas deferens and their ejaculate does not contain testicular products. We measured the concentration of bisphenol A (BPA), bisphenol S (BPS) and bisphenol F (BPF) in the urine and seminal plasma of these men using liquid chromatography tandem mass spectrometry (LC/MSMS). We found that the ratio of urinary and seminal plasma content of bisphenols did not differ in normozoospermic men or men after vasectomy. From the obtained data, it can be concluded that the pathways of transport of bisphenols into seminal plasma are not primarily through the testicular tissue, but this pathway is applied similarly to other routes of transmission by a corresponding ejaculate volume ratio. To a much greater extent than through testicular tissue, bisphenols enter the seminal plasma mainly as part of the secretions of the accessory glands.}, number={6}, journal={TOXICS}, author={Jeseta, Michal and Franzova, Katerina and Machynova, Simona and Kalina, Jiri and Kohoutek, Jiri and Mekinova, Lenka and Crha, Igor and Kempisty, Bartosz and Kasik, Marek and Zakova, Jana and et al.}, year={2022}, month={Jun} }
 @misc{savino_kempisty_mozdziak_2022, title={The Potential of a Protein Model Synthesized Absent of Methionine}, volume={27}, ISSN={["1420-3049"]}, url={https://www.mdpi.com/1420-3049/27/12/3679}, DOI={10.3390/molecules27123679}, abstractNote={Methionine is an amino acid long thought to be essential, but only in the case of protein synthesis initiation. In more recent years, methionine has been found to play an important role in antioxidant defense, stability, and modulation of cell and protein activity. Though these findings have expanded the previously held sentiment of methionine having a singular purpose within cells and proteins, the essential nature of methionine can still be challenged. Many of the features that give methionine its newfound functions are shared by the other sulfur-containing amino acid: cysteine. While the antioxidant, stabilizing, and cell/protein modulatory functions of cysteine have already been well established, recent findings have shown a similar hydrophobicity to methionine which suggests cysteine may be able to replace methionine in all functions outside of protein synthesis initiation with little effect on cell and protein function. Furthermore, a number of novel mechanisms for alternative initiation of protein synthesis have been identified that suggest a potential to bypass the traditional methionine-dependent initiation during times of stress. In this review, these findings are discussed with a number of examples that demonstrate a potential model for synthesizing a protein in the absence of methionine.}, number={12}, journal={MOLECULES}, author={Savino, Ronald J. and Kempisty, Bartosz and Mozdziak, Paul}, year={2022}, month={Jun} }
 @misc{azizidoost_nasrolahi_ghaedrahmati_kempisty_mozdziak_radoszkiewicz_farzaneh_2022, title={The pathogenic roles of lncRNA-Taurine upregulated 1 (TUG1) in colorectal cancer}, volume={22}, ISSN={["1475-2867"]}, DOI={10.1186/s12935-022-02745-1}, abstractNote={Abstract}, number={1}, journal={CANCER CELL INTERNATIONAL}, author={Azizidoost, Shirin and Nasrolahi, Ava and Ghaedrahmati, Farhoodeh and Kempisty, Bartosz and Mozdziak, Paul and Radoszkiewicz, Klaudia and Farzaneh, Maryam}, year={2022}, month={Nov} }
 @article{nawrocki_jopek_kaczmarek_zdun_mozdziak_jemielity_bukowska_kempisty_perek_2022, title={Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture}, volume={13}, ISSN={["2073-4425"]}, url={https://www.mdpi.com/2073-4425/13/7/1205}, DOI={10.3390/genes13071205}, abstractNote={Numerous cardiovascular diseases (CVD) eventually lead to severe myocardial dysfunction, which is the most common cause of death worldwide. A better understanding of underlying molecular mechanisms of cardiovascular pathologies seems to be crucial to develop effective therapeutic options. Therefore, a worthwhile endeavor is a detailed molecular characterization of cells extracted from the myocardium. A transcriptomic profile of atrial cardiomyocytes during long-term primary cell culture revealed the expression patterns depending on the duration of the culture and the heart segment of origin (right atrial appendage and right atrium). Differentially expressed genes (DEGs) were classified as involved in ontological groups such as: “cellular component assembly”, “cellular component organization”, “cellular component biogenesis”, and “cytoskeleton organization”. Transcriptomic profiling allowed us to indicate the increased expression of COL5A2, COL8A1, and COL12A1, encoding different collagen subunits, pivotal in cardiac extracellular matrix (ECM) structure. Conversely, genes important for cellular architecture, such as ABLIM1, TMOD1, XIRP1, and PHACTR1, were downregulated during in vitro culture. The culture conditions may create a favorable environment for reconstruction of the ECM structures, whereas they may be suboptimal for expression of some pivotal transcripts responsible for the formation of intracellular structures.}, number={7}, journal={GENES}, author={Nawrocki, Mariusz J. and Jopek, Karol and Kaczmarek, Mariusz and Zdun, Maciej and Mozdziak, Paul and Jemielity, Marek and Bukowska, Dorota and Kempisty, Bartosz and Perek, Bartlomiej}, year={2022}, month={Jul} }
 @article{nawrocki_jopek_zdun_mozdziak_jemielity_perek_bukowska_kempisty_2021, title={Expression Profile of Genes Encoding Proteins Involved in Regulation of Vasculature Development and Heart Muscle Morphogenesis-A Transcriptomic Approach Based on a Porcine Model}, volume={22}, ISSN={["1422-0067"]}, url={https://www.mdpi.com/1422-0067/22/16/8794}, DOI={10.3390/ijms22168794}, abstractNote={Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, understanding the mechanisms that control the regeneration of the heart muscle is important. The development of new coronary vessels plays a pivotal role in cardiac regeneration. Employing microarray expression assays and RT-qPCR validation expression pattern of genes in long-term primary cultured cells isolated form the right atrial appendage (RAA) and right atrium (RA) was evaluated. After using DAVID software, it indicated the analysis expression profiles of genes involved in ontological groups such as: “angiogenesis”, “blood vessel morphogenesis”, “circulatory system development”, “regulation of vasculature development”, and “vasculature development” associated with the process of creation new blood vessels. The performed transcriptomic comparative analysis between two different compartments of the heart muscle allowed us to indicate the presence of differences in the expression of key transcripts depending on the cell source. Increases in culture intervals significantly increased expression of SFRP2, PRRX1 genes and some other genes involved in inflammatory process, such as: CCL2, IL6, and ROBO1. Moreover, the right atrial appendage gene encoding lysyl oxidase (LOX) showed much higher expression compared to the pre-cultivation state.}, number={16}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Nawrocki, Mariusz J. and Jopek, Karol and Zdun, Maciej and Mozdziak, Paul and Jemielity, Marek and Perek, Bartlomiej and Bukowska, Dorota and Kempisty, Bartosz}, year={2021}, month={Aug} }
 @article{chermula_hutchings_kranc_jozkowiak_jopek_stelmach_mozdziak_pawelczyk_piotrowska-kempisty_spaczynski_et al._2021, title={Expression Profile of New Gene Markers and Signaling Pathways Involved in Immunological Processes in Human Cumulus-Oophorus Cells}, volume={12}, ISSN={["2073-4425"]}, url={https://www.mdpi.com/2073-4425/12/9/1369}, DOI={10.3390/genes12091369}, abstractNote={The function of the immune system extends from defense against external pathogens to the recognition and elimination of mutated or dying cells, aiding elimination of malignant potential and/or maintaining homeostasis. The many cell types of the immune system secrete a broad range of factors to enable cellular signaling that is vital to physiological processes. Additionally, in the ovary, follicular selection and maturation, as well as ovulation, are directly regulated by the nearby immune cells. Additionally, ovulation and rupture of the follicle have been observed to resemble a local inflammatory response. Cells of the cumulus–oocyte complex (COC) show evolving gene expression profiles throughout the oocytes’ lifespan, including genes associated with immunological processes. Analysis of these genes allows the identification of useful molecular markers, as well as highlighting gene functions and interactions in these cells. Cumulus cells were obtained from hormonally stimulated patients undergoing an in vitro fertilization procedure and studied under long-term culture conditions. The microarray technique made it possible to compare the level of CCs’ gene expression on the 1st, 7th, 15th and 30th day of cultivation. Additionally, RNA microarray analysis was performed to map gene expression in these cells, associated with immunological processes and associated cytokine signaling. Subsequently, the use of DAVID software allowed us to identify the “defense response to other organism”, “defense response”, “defense response to virus”, “cytokine secretion”, “cytokine production” and “cytokine-mediated signaling pathway” GO BP terms, as well as allowing further analysis of the most differentially expressed genes associated with these processes. Of the 122 genes involved, 121 were upregulated and only one was downregulated. The seven most upregulated genes related to the abovementioned terms were ANXA3, IFIT1, HLA-DPA1, MX1, KRT8, HLA-DRA and KRT18. Therefore, genes involved in immunological defense processes are upregulated in CC cultures and could serve as useful molecular markers of growth and development in the COC, as well as the proliferation of granulosa and cumulus cells.}, number={9}, journal={GENES}, author={Chermula, Blazej and Hutchings, Greg and Kranc, Wieslawa and Jozkowiak, Malgorzata and Jopek, Karol and Stelmach, Boguslawa and Mozdziak, Paul and Pawelczyk, Leszek and Piotrowska-Kempisty, Hanna and Spaczynski, Robert Z. and et al.}, year={2021}, month={Sep} }
 @article{jankowski_kaczmarek_wasiatycz_dompe_mozdziak_jaskowski_piotrowska-kempisty_kempisty_2021, title={Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts}, volume={22}, ISSN={["1422-0067"]}, url={https://www.mdpi.com/1422-0067/22/13/6663}, DOI={10.3390/ijms22136663}, abstractNote={Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine.}, number={13}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Jankowski, Maurycy and Kaczmarek, Mariusz and Wasiatycz, Grzegorz and Dompe, Claudia and Mozdziak, Paul and Jaskowski, Jedrzej M. and Piotrowska-Kempisty, Hanna and Kempisty, Bartosz}, year={2021}, month={Jul} }
 @misc{dompe_kulus_stefanska_kranc_chermula_bryl_pienkowski_nawrocki_petitte_stelmach_et al._2021, title={Human Granulosa Cells-Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis}, volume={10}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/10/6/1396}, DOI={10.3390/cells10061396}, abstractNote={The ovarian follicle is the basic functional unit of the ovary, comprising theca cells and granulosa cells (GCs). Two different types of GCs, mural GCs and cumulus cells (CCs), serve different functions during folliculogenesis. Mural GCs produce oestrogen during the follicular phase and progesterone after ovulation, while CCs surround the oocyte tightly and form the cumulus oophurus and corona radiata inner cell layer. CCs are also engaged in bi-directional metabolite exchange with the oocyte, as they form gap-junctions, which are crucial for both the oocyte’s proper maturation and GC proliferation. However, the function of both GCs and CCs is dependent on proper follicular angiogenesis. Aside from participating in complex molecular interplay with the oocyte, the ovarian follicular cells exhibit stem-like properties, characteristic of mesenchymal stem cells (MSCs). Both GCs and CCs remain under the influence of various miRNAs, and some of them may contribute to polycystic ovary syndrome (PCOS) or premature ovarian insufficiency (POI) occurrence. Considering increasing female fertility problems worldwide, it is of interest to develop new strategies enhancing assisted reproductive techniques. Therefore, it is important to carefully consider GCs as ovarian stem cells in terms of the cellular features and molecular pathways involved in their development and interactions as well as outline their possible application in translational medicine.}, number={6}, journal={CELLS}, publisher={MDPI AG}, author={Dompe, Claudia and Kulus, Magdalena and Stefanska, Katarzyna and Kranc, Wieslawa and Chermula, Blazej and Bryl, Rut and Pienkowski, Wojciech and Nawrocki, Mariusz J. and Petitte, James N. and Stelmach, Boguslawa and et al.}, year={2021}, month={Jun} }
 @article{kulus_sibiak_stefanska_zdun_wieczorkiewicz_piotrowska-kempisty_jaskowski_bukowska_ratajczak_zabel_et al._2021, title={Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues-Origins, Characteristics, Signaling Pathways, and Clinical Trials}, volume={10}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/10/12/3278}, DOI={10.3390/cells10123278}, abstractNote={Mesenchymal stem/stromal cells (MSCs) are currently one of the most extensively researched fields due to their promising opportunity for use in regenerative medicine. There are many sources of MSCs, of which cells of perinatal origin appear to be an invaluable pool. Compared to embryonic stem cells, they are devoid of ethical conflicts because they are derived from tissues surrounding the fetus and can be safely recovered from medical waste after delivery. Additionally, perinatal MSCs exhibit better self-renewal and differentiation properties than those derived from adult tissues. It is important to consider the anatomy of perinatal tissues and the general description of MSCs, including their isolation, differentiation, and characterization of different types of perinatal MSCs from both animals and humans (placenta, umbilical cord, amniotic fluid). Ultimately, signaling pathways are essential to consider regarding the clinical applications of MSCs. It is important to consider the origin of these cells, referring to the anatomical structure of the organs of origin, when describing the general and specific characteristics of the different types of MSCs as well as the pathways involved in differentiation.}, number={12}, journal={CELLS}, author={Kulus, Magdalena and Sibiak, Rafal and Stefanska, Katarzyna and Zdun, Maciej and Wieczorkiewicz, Maria and Piotrowska-Kempisty, Hanna and Jaskowski, Jedrzej M. and Bukowska, Dorota and Ratajczak, Kornel and Zabel, Maciej and et al.}, year={2021}, month={Dec} }
 @misc{hutchings_kruszyna_nawrocki_strauss_bryl_spaczynska_perek_jemielity_mozdziak_kempisty_et al._2021, title={Molecular Mechanisms Associated with ROS-Dependent Angiogenesis in Lower Extremity Artery Disease}, volume={10}, ISSN={["2076-3921"]}, url={https://www.mdpi.com/2076-3921/10/5/735}, DOI={10.3390/antiox10050735}, abstractNote={Currently, atherosclerosis, which affects the vascular bed of all vital organs and tissues, is considered as a leading cause of death. Most commonly, atherosclerosis involves coronary and peripheral arteries, which results in acute (e.g., myocardial infarction, lower extremities ischemia) or chronic (persistent ischemia leading to severe heart failure) consequences. All of them have a marked unfavorable impact on the quality of life and are associated with increased mortality and morbidity in human populations. Lower extremity artery disease (LEAD, also defined as peripheral artery disease, PAD) refers to atherosclerotic occlusive disease of the lower extremities, where partial or complete obstruction of peripheral arteries is observed. Decreased perfusion can result in ischemic pain, non-healing wounds, and ischemic ulcers, and significantly reduce the quality of life. However, the progressive atherosclerotic changes cause stimulation of tissue response processes, like vessel wall remodeling and neovascularization. These mechanisms of adapting the vascular network to pathological conditions seem to play a key role in reducing the impact of the changes limiting the flow of blood. Neovascularization as a response to ischemia induces sprouting and expansion of the endothelium to repair and grow the vessels of the circulatory system. Neovascularization consists of three different biological processes: vasculogenesis, angiogenesis, and arteriogenesis. Both molecular and environmental factors that may affect the process of development and growth of blood vessels were analyzed. Particular attention was paid to the changes taking place during LEAD. It is important to consider the molecular mechanisms underpinning vessel growth. These mechanisms will also be examined in the context of diseases commonly affecting blood vessel function, or those treatable in part by manipulation of angiogenesis. Furthermore, it may be possible to induce the process of blood vessel development and growth to treat peripheral vascular disease and wound healing. Reactive oxygen species (ROS) play an important role in regulation of essential cellular signaling pathways such as cell differentiation, proliferation, migration and apoptosis. With regard to the repair processes taking place during diseases such as LEAD, prospective therapeutic methods have been described that could significantly improve the treatment of vessel diseases in the future. Summarizing, regenerative medicine holds the potential to transform the therapeutic methods in heart and vessel diseases treatment.}, number={5}, journal={ANTIOXIDANTS}, author={Hutchings, Greg and Kruszyna, Lukasz and Nawrocki, Mariusz J. and Strauss, Ewa and Bryl, Rut and Spaczynska, Julia and Perek, Bartlomiej and Jemielity, Marek and Mozdziak, Paul and Kempisty, Bartosz and et al.}, year={2021}, month={May} }
 @article{kulus_kranc_wojtanowicz-markiewicz_celichowski_swiatly-blaszkiewicz_matuszewska_sujka-kordowska_konwerska_zdun_bryl_et al._2021, title={New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes}, volume={22}, ISSN={["1422-0067"]}, url={https://www.mdpi.com/1422-0067/22/4/2082}, DOI={10.3390/ijms22042082}, abstractNote={Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction.}, number={4}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Kulus, Magdalena and Kranc, Wieslawa and Wojtanowicz-Markiewicz, Katarzyna and Celichowski, Piotr and Swiatly-Blaszkiewicz, Agata and Matuszewska, Eliza and Sujka-Kordowska, Patrycja and Konwerska, Aneta and Zdun, Maciej and Bryl, Rut and et al.}, year={2021}, month={Feb} }
 @misc{nowicki_kulus_wieczorkiewicz_pienkowski_stefanska_skupin-mrugalska_bryl_mozdziak_kempisty_piotrowska-kempisty_2021, title={Ovarian Cancer and Cancer Stem Cells-Cellular and Molecular Characteristics, Signaling Pathways, and Usefulness as a Diagnostic Tool in Medicine and Oncology}, volume={13}, ISSN={["2072-6694"]}, url={https://www.mdpi.com/2072-6694/13/16/4178}, DOI={10.3390/cancers13164178}, abstractNote={Despite the increasing development of medicine, ovarian cancer is still a high-risk, metastatic disease that is often diagnosed at a late stage. In addition, difficulties in its treatment are associated with high resistance to chemotherapy and frequent relapse. Cancer stem cells (CSCs), recently attracting significant scientific interest, are considered to be responsible for the malignant features of tumors. CSCs, as the driving force behind tumor development, generate new cells by modifying different signaling pathways. Moreover, investigations on different types of tumors have shown that signaling pathways are key to epithelial-mesenchymal transition (EMT) regulation, metastasis, and self-renewal of CSCs. Based on these established issues, new therapies are being investigated based on the use of inhibitors to block CSC growth and proliferation signals. Many reports indicate that CSC markers play a key role in cancer metastasis, with hopes placed in their targeting to block this process and eliminate relapses. Current histological classification of ovarian tumors, their epidemiology, and the most recent knowledge of ovarian CSCs, with particular emphasis on their molecular background, are important aspects for consideration. Furthermore, the importance of signaling pathways involved in tumor growth, development, and metastasis, is also presented.}, number={16}, journal={CANCERS}, author={Nowicki, Andrzej and Kulus, Magdalena and Wieczorkiewicz, Maria and Pienkowski, Wojciech and Stefanska, Katarzyna and Skupin-Mrugalska, Paulina and Bryl, Rut and Mozdziak, Paul and Kempisty, Bartosz and Piotrowska-Kempisty, Hanna}, year={2021}, month={Aug} }
 @article{jeseta_navratilova_franzova_fialkova_kempisty_ventruba_zakova_crha_2021, title={Overview of the Mechanisms of Action of Selected Bisphenols and Perfluoroalkyl Chemicals on the Male Reproductive Axes}, volume={12}, ISSN={["1664-8021"]}, DOI={10.3389/fgene.2021.692897}, abstractNote={Male fertility has been deteriorating worldwide for considerable time, with the greatest deterioration recorded mainly in the United States, Europe countries, and Australia. That is, especially in countries where an abundance of chemicals called endocrine disruptors has repeatedly been reported, both in the environment and in human matrices. Human exposure to persistent and non-persistent chemicals is ubiquitous and associated with endocrine-disrupting effects. This group of endocrine disrupting chemicals (EDC) can act as agonists or antagonists of hormone receptors and can thus significantly affect a number of physiological processes. It can even negatively affect human reproduction with an impact on the development of gonads and gametogenesis, fertilization, and the subsequent development of embryos. The negative effects of endocrine disruptors on sperm gametogenesis and male fertility in general have been investigated and repeatedly demonstrated in experimental and epidemiological studies. Male reproduction is affected by endocrine disruptors via their effect on testicular development, impact on estrogen and androgen receptors, potential epigenetic effect, production of reactive oxygen species or direct effect on spermatozoa and other cells of testicular tissue. Emerging scientific evidence suggests that the increasing incidence of male infertility is associated with the exposure to persistent and non-persistent endocrine-disrupting chemicals such as bisphenols and perfluoroalkyl chemicals (PFAS). These chemicals may impact men’s fertility through various mechanisms. This study provides an overview of the mechanisms of action common to persistent (PFAS) and nonpersistent (bisphenols) EDC on male fertility.}, journal={FRONTIERS IN GENETICS}, author={Jeseta, Michal and Navratilova, Jana and Franzova, Katerina and Fialkova, Sandra and Kempisty, Bartozs and Ventruba, Pavel and Zakova, Jana and Crha, Igor}, year={2021}, month={Sep} }
 @article{kocherova_bryja_blochowiak_kaczmarek_stefanska_matys_grzech-lesniak_dominiak_mozdziak_kempisty_et al._2021, title={Photobiomodulation with Red and Near-Infrared Light Improves Viability and Modulates Expression of Mesenchymal and Apoptotic-Related Markers in Human Gingival Fibroblasts}, volume={14}, ISSN={["1996-1944"]}, url={https://www.mdpi.com/1996-1944/14/12/3427}, DOI={10.3390/ma14123427}, abstractNote={Photobiomodulation (PBM), also called low-level laser treatment (LLLT), has been considered a promising tool in periodontal treatment due to its anti-inflammatory and wound healing properties. However, photobiomodulation’s effectiveness depends on a combination of parameters, such as energy density, the duration and frequency of the irradiation sessions, and wavelength, which has been shown to play a key role in laser-tissue interaction. The objective of the study was to compare the in vitro effects of two different wavelengths—635 nm and 808 nm—on the human primary gingival fibroblasts in terms of viability, oxidative stress, inflammation markers, and specific gene expression during the four treatment sessions at power and energy density widely used in dental practice (100 mW, 4 J/cm2). PBM with both 635 and 808 nm at 4 J/cm2 increased the cell number, modulated extracellular oxidative stress and inflammation markers and decreased the susceptibility of human primary gingival fibroblasts to apoptosis through the downregulation of apoptotic-related genes (P53, CASP9, BAX). Moreover, modulation of mesenchymal markers expression (CD90, CD105) can reflect the possible changes in the differentiation status of irradiated fibroblasts. The most pronounced results were observed following the third irradiation session. They should be considered for the possible optimization of existing low-level laser irradiation protocols used in periodontal therapies.}, number={12}, journal={MATERIALS}, author={Kocherova, Ievgeniia and Bryja, Artur and Blochowiak, Katarzyna and Kaczmarek, Mariusz and Stefanska, Katarzyna and Matys, Jacek and Grzech-Lesniak, Kinga and Dominiak, Marzena and Mozdziak, Paul and Kempisty, Bartosz and et al.}, year={2021}, month={Jun} }
 @article{saska_pilatti_silva_nagasawa_camara_lizier_finger_dyszkiewicz konwinska_kempisty_tunchel_et al._2021, title={Polydioxanone-Based Membranes for Bone Regeneration}, volume={13}, ISSN={["2073-4360"]}, DOI={10.3390/polym13111685}, abstractNote={Resorbable synthetic and natural polymer-based membranes have been extensively studied for guided tissue regeneration. Alloplastic biomaterials are often used for tissue regeneration due to their lower immunoreactivity when compared with allogeneic and xenogeneic materials. Plenum® Guide is a synthetic membrane material based on polydioxanone (PDO), whose surface morphology closely mimics the extracellular matrix. In this study, Plenum® Guide was compared with collagen membranes as a barrier material for bone-tissue regeneration in terms of acute and subchronic systemic toxicity. Moreover, characterizations such as morphology, thermal analysis (Tm = 107.35 °C and crystallinity degree = 52.86 ± 2.97 %, final product), swelling (thickness: 0.25 mm ≅ 436% and 0.5 mm ≅ 425% within 24 h), and mechanical tests (E = 30.1 ± 6.25 MPa; σ = 3.92 ± 0.28 MPa; ε = 287.96 ± 34.68%, final product) were performed. The in vivo results revealed that the PDO membranes induced a slightly higher quantity of newly formed bone tissue than the control group (score: treated group = 15, control group = 13) without detectable systemic toxicity (clinical signs and evaluation of the membranes after necropsy did not result in differences between groups, i.e., non-reaction -> tissue-reaction index = 1.3), showing that these synthetic membranes have the essential characteristics for an effective tissue regeneration. Human adipose-derived stem cells (hASCs) were seeded on PDO membranes; results demonstrated efficient cell migration, adhesion, spread, and proliferation, such that there was a slightly better hASC osteogenic differentiation on PDO than on collagen membranes. Hence, Plenum® Guide membranes are a safe and efficient alternative for resorbable membranes for tissue regeneration.}, number={11}, journal={POLYMERS}, author={Saska, Sybele and Pilatti, Livia and Silva, Edvaldo Santos de Sousa and Nagasawa, Magda Aline and Camara, Diana and Lizier, Nelson and Finger, Eduardo and Dyszkiewicz Konwinska, Marta and Kempisty, Bartosz and Tunchel, Samy and et al.}, year={2021}, month={Jun} }
 @misc{kulus_kulus_stefanska_sobolewski_piotrowska-kempisty_mozdziak_kempisty_2021, title={SARS-CoV-2 Genetic Variability and Non-Specific Immunity Associated with the Use of Different BCG Strains-A Molecular and Clinical Approach}, volume={9}, ISSN={["2076-393X"]}, url={https://www.mdpi.com/2076-393X/9/6/639}, DOI={10.3390/vaccines9060639}, abstractNote={The effect of BCG vaccination against tuberculosis on the reduction in COVID-19 infection is related to the effect of the BCG vaccine on the immunomodulation of non-specific immunity. In the early stages of the pandemic, countries with universal BCG vaccination programs registered a low number of new cases of COVID-19, with the situation now reversed, as exemplified by India. The high genetic variability of SARS-CoV-2, a known characteristic of RNA viruses, causing the occurrence of SARS-CoV-2 variants may have led to the virus adapting to overcome the initial immune protection. The strains from the United Kingdom (B1.1.7), Brazil (B1.1.28 and B1.1.33), South Africa (B.1.351), and India (B.1.617) are characterized by a greater ability to spread in the environment, in comparison with the original infectious agent of SARS-CoV-2. It should be remembered that the large variation in the genetic makeup of SARS-CoV-2 may result in future changes in its pathogenicity, immunogenicity and antigenicity, and therefore it is necessary to carefully study the mutations occurring within the virus to determine whether the current vaccines will remain effective. However, most studies show that monoclonal antibodies produced after vaccination against COVID-19 are effective against the newly developed variants.}, number={6}, journal={VACCINES}, author={Kulus, Jakub and Kulus, Magdalena and Stefanska, Katarzyna and Sobolewski, Jaroslaw and Piotrowska-Kempisty, Hanna and Mozdziak, Paul and Kempisty, Bartosz}, year={2021}, month={Jun} }
 @article{borowiec_angelova volponi_mozdziak_kempisty_dyszkiewicz-konwinska_2021, title={Small Extracellular Vesicles and COVID19-Using the "Trojan Horse" to Tackle the Giant}, volume={10}, ISSN={["2073-4409"]}, url={https://www.mdpi.com/2073-4409/10/12/3383}, DOI={10.3390/cells10123383}, abstractNote={The COVID-19 pandemic is a global challenge, demanding researchers address different approaches in relation to prevention, diagnostics and therapeutics. Amongst the many tactics of tackling these therapeutic challenges, small extracellular vesicles (sEVs) or exosomes are emerging as a new frontier in the field of ameliorating viral infections. Exosomes are part of extracellular vesicles (EVs)—spherical biological structures with a lipid bilayer of a diameter of up to 5000 nm, which are released into the intercellular space by most types of eukaryotic cells, both in physiological and pathological states. EVs share structural similarities to viruses, such as small size, common mechanisms of biogenesis and mechanisms for cell entry. The role of EVs in promoting the viral spread by evading the immune response of the host, which is exhibited by retroviruses, indicates the potential for further investigation and possible manipulation of these processes when tackling the spread and treatment of COVID-19. The following paper introduces the topic of the use of exosomes in the treatment of viral infections, and presents the future prospects for the use of these EVs.}, number={12}, journal={CELLS}, author={Borowiec, Blanka Maria and Angelova Volponi, Ana and Mozdziak, Paul and Kempisty, Bartosz and Dyszkiewicz-Konwinska, Marta}, year={2021}, month={Dec} }
 @article{kulus_kulus_kranc_jopek_zdun_jozkowiak_jaskowski_piotrowska-kempisty_bukowska_antosik_et al._2021, title={Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells}, volume={10}, ISSN={["2079-7737"]}, url={https://www.mdpi.com/2079-7737/10/11/1214}, DOI={10.3390/biology10111214}, abstractNote={The extracellular matrix (ECM) in granulosa cells is functionally very important, and it is involved in many processes related to ovarian follicle growth and ovulation. The aim of this study was to describe the expression profile of genes within granulosa cells that are associated with extracellular matrix formation, intercellular signaling, and cell–cell fusion. The material for this study was ovaries of sexually mature pigs obtained from a commercial slaughterhouse. Laboratory-derived granulosa cells (GCs) from ovarian follicles were cultured in a primary in vitro culture model. The extracted genetic material (0, 48, 96, and 144 h) were subjected to microarray expression analysis. Among 81 genes, 66 showed increased expression and only 15 showed decreased expression were assigned to 7 gene ontology groups “extracellular matrix binding”, “extracellular matrix structural constituent”, “binding, bridging”, “cadherin binding”, “cell adhesion molecule binding”, “collagen binding” and “cadherin binding involved in cell-cell adhesion”. The 10 genes with the highest expression (POSTN, ITGA2, FN1, LAMB1, ITGB3, CHI3L1, PCOLCE2, CAV1, DCN, COL14A1) and 10 of the most down-regulated (SPP1, IRS1, CNTLN, TMPO, PAICS, ANK2, ADAM23, ABI3BP, DNAJB1, IGF1) were selected for further analysis. The results were validated by RT-qPCR. The current results may serve as preliminary data for further analyses using in vitro granulosa cell cultures in assisted reproduction technologies, studies of pathological processes in the ovary as well as in the use of the stemness potential of GCs.}, number={11}, journal={BIOLOGY-BASEL}, author={Kulus, Jakub and Kulus, Magdalena and Kranc, Wieslawa and Jopek, Karol and Zdun, Maciej and Jozkowiak, Malgorzata and Jaskowski, Jedrzej M. and Piotrowska-Kempisty, Hanna and Bukowska, Dorota and Antosik, Pawel and et al.}, year={2021}, month={Nov} }