@article{turner_twiddy_wilkins_ramesh_kilgour_domingos_nasrallah_menegatti_daniele_2023, title={Biodegradable elastomeric circuit boards from citric acid-based polyesters}, volume={7}, ISSN={["2397-4621"]}, DOI={10.1038/s41528-023-00258-z}, abstractNote={Abstract}, number={1}, journal={NPJ FLEXIBLE ELECTRONICS}, author={Turner, Brendan L. and Twiddy, Jack and Wilkins, Michael D. and Ramesh, Srivatsan and Kilgour, Katie M. and Domingos, Eleo and Nasrallah, Olivia and Menegatti, Stefano and Daniele, Michael A.}, year={2023}, month={Jun} }
 @article{kilgour_turner_daniele_menegatti_2023, title={One-Step Quantification of anti-Covid-19 Antibodies via Dual Affinity Ratiometric Quenching Assays}, volume={6}, ISSN={["1520-6882"]}, DOI={10.1021/acs.analchem.3c01266}, abstractNote={The global pandemic caused by acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people and paralyzed healthcare systems worldwide. Developing rapid and accurate tests to detect and quantify anti-SARS-CoV-2 antibodies in complex fluids is critical to (i) track and address the spread of SARS-CoV-2 variants with different virulence and (ii) support the industrial manufacturing and clinical administration of anti-SARS-CoV-2 therapeutic antibodies. Conventional immunoassays, such as lateral flow, ELISA, and surface plasmon resonance (SPR), are either qualitative or, when quantitative, are laborious and expensive and suffer from high variability. Responding to these challenges, this study evaluates the performance of the Dual-Affinity Ratiometric Quenching (DARQ) assay for the quantification of anti-SARS-CoV-2 antibodies in bioprocess harvests and intermediate fractions (i.e., a Chinese hamster ovary (CHO) cell culture supernatant and a purified eluate) and human fluids (i.e., saliva and plasma). Monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid as well as the spike protein of the delta and omicron variants are adopted as model analytes. Additionally, conjugate pads loaded with dried protein were studied as an at-line quantification method that can be used in clinical or manufacturing laboratories. Our results indicate that the DARQ assay is a highly reproducible (coefficient of variation ∼0.5-3%) and rapid (<10 min) test, whose sensitivity (∼0.23-2.5 ng/mL), limit of detection (23-250 ng/mL), and dynamic range (70-1300 ng/mL) are independent of sample complexity, thus representing a valuable tool for monitoring anti-SARS-CoV-2 antibodies.}, journal={ANALYTICAL CHEMISTRY}, author={Kilgour, Katie M. and Turner, Brendan L. and Daniele, Michael and Menegatti, Stefano}, year={2023}, month={Jun} }
 @article{turner_ramesh_menegatti_daniele_2021, title={Resorbable elastomers for implantable medical devices: highlights and applications}, volume={12}, ISSN={["1097-0126"]}, DOI={10.1002/pi.6349}, abstractNote={Abstract}, journal={POLYMER INTERNATIONAL}, author={Turner, Brendan and Ramesh, Srivatsan and Menegatti, Stefano and Daniele, Michael}, year={2021}, month={Dec} }
 @article{turner_senevirathne_kilgour_mcart_biggs_menegatti_daniele_2021, title={Ultrasound-Powered Implants: A Critical Review of Piezoelectric Material Selection and Applications}, volume={7}, ISSN={["2192-2659"]}, DOI={10.1002/adhm.202100986}, abstractNote={Abstract}, journal={ADVANCED HEALTHCARE MATERIALS}, author={Turner, Brendan L. and Senevirathne, Seedevi and Kilgour, Katie and McArt, Darragh and Biggs, Manus and Menegatti, Stefano and Daniele, Michael A.}, year={2021}, month={Jul} }
 @article{turner_kilgour_stine_daniele_menegatti_2020, title={Dual-Affinity Ratiometric Quenching (DARQ) Assay for the Quantification of Therapeutic Antibodies in CHO-S Cell Culture Fluids}, volume={92}, ISSN={["1520-6882"]}, DOI={10.1021/acs.analchem.0c04269}, abstractNote={More than 100 monoclonal antibodies (mAbs) are in industrial and clinical development to treat myriad diseases. Accurate quantification of mAbs in complex media, derived from industrial and patient samples, is vital to determine production efficiency or pharmacokinetic properties. To date, mAb quantification requires time and labor-intensive assays. Herein, we report a novel dual-affinity ratiometric quenching (DARQ) assay, which combines selective biorecognition and quenching of fluorescence signals for rapid and sensitive quantification of therapeutic monoclonal antibodies (mAbs). The reported assay relies on the affinity complexation of the target mAb by the corresponding antigens and Protein L (PrL, which targets the Fab region of the antibody), respectively, labeled with fluorescein and rhodamine. Within the affinity complex, the mAb acts as a scaffold framing the labeled affinity tags (PrL and antigen) in a molecular proximity that results in ratiometric quenching of their fluorescence emission. Notably, the decrease in fluorescence emission intensity is linearly dependent upon mAb concentration in solution. Control experiments conducted with one affinity tag only, two tags labeled with equal fluorophores, or two tags labeled with fluorophores of discrete absorbance and emission bands exhibited significantly reduced effect. The assay was evaluated in noncompetitive (pure mAb) and competitive conditions (mAb in a Chinese Hamster Ovary (CHO) cell culture harvest). The "DARQ" assay is highly reproducible (coefficient of variation ∼0.8-0.7%) and rapid (5 min), and its sensitivity (∼0.2-0.5 ng·mL-1), limit of detection (75-119 ng·mL-1), and dynamic range (300-1600 ng·mL-1) are independent of the presence of CHO host cell proteins.}, number={24}, journal={ANALYTICAL CHEMISTRY}, author={Turner, Brendan L. and Kilgour, Katie M. and Stine, Sydney J. and Daniele, Michael and Menegatti, Stefano}, year={2020}, month={Dec}, pages={16274–16283} }
 @article{yuen_pozdin_young_turner_giles_naciri_trammell_charles_stenger_daniele_2020, title={Perylene-diimide-based n-type semiconductors with enhanced air and temperature stable photoconductor and transistor properties}, volume={174}, ISSN={["1873-3743"]}, DOI={10.1016/j.dyepig.2019.108014}, abstractNote={We report the synthesis and characterization of highly air and temperature stable, solution-processed, n-type organic semiconductors: a perylene-diimide monomer and a perylene-diimide-based pendant polymer. When integrated into a transistor structure, both materials possess pure n-type transport with mobility as high as 10−5 cm2 V−1 s−1 for the polymer. The organic semiconductors exhibit good photoconductor properties, with photocurrent to dark current ratios of up to 103 for the monomer, despite its lower FET mobility. The differences in transistor and photoconductor properties suggest different applications for each material. Both materials can be processed in air, and their transport properties have good air stability, improving with annealing even up to 200 °C in air. It is notable that such air-stable photoconductivity and transport properties have rarely been reported for n-type organic semiconductors before, as most n-type organic semiconductors are not stable in air. Hence, these materials may have potential in a wide range of applications.}, journal={DYES AND PIGMENTS}, author={Yuen, Jonathan D. and Pozdin, Vladimir A. and Young, Ashlyn T. and Turner, Brendan L. and Giles, Ian D. and Naciri, Jawad and Trammell, Scott A. and Charles, Paul T. and Stenger, David A. and Daniele, Michael A.}, year={2020}, month={Mar} }