Balaji Rao
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Works (55)
Updated: April 3rd, 2024 21:00
2023 journal article
Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions
JOURNAL OF BIOLOGICAL CHEMISTRY, 299(5).
MeSH headings : Humans; Cell Differentiation / drug effects; Colforsin / pharmacology; Colforsin / metabolism; HLA-G Antigens / genetics; HLA-G Antigens / metabolism; Laminin / pharmacology; Stem Cells / cytology; Stem Cells / drug effects; Transforming Growth Factor beta / metabolism; Trophoblasts / cytology; Trophoblasts / metabolism; Culture Media / chemistry; Hypoxia-Inducible Factor 1, alpha Subunit / genetics; Gene Expression Regulation, Developmental / drug effects; Cytological Techniques / methods
www.ncbi.nlm.nih.gov (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
5. Gender Equality
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 3, 2023
2022 article
Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase
McArthur, N., Cruz-Teran, C., Thatavarty, A., Reeves, G. T., & Rao, B. M. (2022, July 1). ACS OMEGA.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: July 26, 2022
2022 journal article
Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase
ACS OMEGA, 7(28), 24551–24560.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 8, 2022
2022 journal article
Modified Histone Peptides Linked to Magnetic Beads Reduce Binding Specificity
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(3).
author keywords: yeast surface display; histone post-translational modifications; affinity reagents; protein engineering; chromodomain; binder; reader; antibody; epigenome engineering; synthetic biology
MeSH headings : HEK293 Cells; Histone Code; Histones / metabolism; Humans; Jurkat Cells; K562 Cells; Protein Array Analysis; Protein Binding; Protein Engineering; Protein Processing, Post-Translational; Saccharomyces cerevisiae
TL;DR:
Specific experimental conditions that affect binding specificities of post-translationally modified histones in classic protein engineering platforms are described and likely explain the relative difficulty with histone targets in these platforms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 21, 2022
2021 journal article
Mapping the residue specificities of epigenome enzymes by yeast surface display
CELL CHEMICAL BIOLOGY, 28(12), 1772-+.
MeSH headings : E1A-Associated p300 Protein / genetics; E1A-Associated p300 Protein / metabolism; Epigenome / genetics; Humans; Saccharomyces cerevisiae / metabolism
TL;DR:
The results show that using yeast surface display to study histone modifications is a useful tool that can advance the understanding of chromatin biology by enabling efficient interrogation of the complexity of epigenome modifications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: January 3, 2022
2021 journal article
Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions
ACS SYNTHETIC BIOLOGY, 10(3), 505–514.
author keywords: yeast two-hybrid; protein-protein interactions; protein engineering; yeast surface display; post-translational modification; cDNA library
MeSH headings : Gene Library; Genes, Reporter; Protein Binding; Protein Domains; Protein Interaction Maps; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Smad3 Protein / chemistry; Smad3 Protein / metabolism; Transcription Factors / chemistry; Transcription Factors / metabolism; Two-Hybrid System Techniques
TL;DR:
A quantitative yeast-yeast two-hybrid system that not only enables the discovery of specific protein-protein interactions but also efficient, quantitative estimation of their binding affinities (KD), and it was shown that qYY2H could also quantitatively evaluate binding interactions mediated by post-translational modifications on the bait protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 29, 2021
2021 journal article
Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4).
author keywords: yeast display library; cyclic peptide; Yes Associated Protein (YAP) 65; WW domain; transglutaminase
MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Adaptor Proteins, Signal Transducing / metabolism; Albumins / metabolism; Binding Sites; Combinatorial Chemistry Techniques; Endoplasmic Reticulum / metabolism; Flow Cytometry; Ligands; Muramidase / metabolism; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / pharmacology; Protein Binding; Protein Engineering / methods; Transcription Factors / chemistry; Transcription Factors / metabolism; Transglutaminases / metabolism; YAP-Signaling Proteins; Yeasts / genetics; Yeasts / growth & development
TL;DR:
The usefulness of yeast surface display for screening transglutaminase-cyclized peptide libraries, and efficient identification of cyclic peptide ligands, are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 22, 2021
2021 journal article
Two distinct trophectoderm lineage stem cells from human pluripotent stem cells
JOURNAL OF BIOLOGICAL CHEMISTRY, 296.
MeSH headings : CDX2 Transcription Factor / metabolism; Cell Culture Techniques; Cell Differentiation; Cell Lineage; Culture Media; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Female; Humans; Placenta / cytology; Placenta / metabolism; Pluripotent Stem Cells / cytology; Pluripotent Stem Cells / metabolism; Pregnancy; Trophoblasts / cytology; Trophoblasts / metabolism
TL;DR:
Derivation of hTSCs and hTESCs from pluripotent stem cells significantly enables construction of models for normal and pathological placental development.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 7, 2021
2020 journal article
A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells
ELIFE, 9.
MeSH headings : Animals; Biosensing Techniques; Cell Line; Cell Physiological Phenomena / genetics; Fluorescence Resonance Energy Transfer; Fluorescent Dyes / metabolism; HEK293 Cells; Humans; Magnetic Resonance Spectroscopy; Mice; Phosphorylation / genetics; Proto-Oncogene Proteins c-fyn / chemistry; Proto-Oncogene Proteins c-fyn / genetics; Proto-Oncogene Proteins c-fyn / metabolism; Signal Transduction / genetics; Yeasts / genetics
TL;DR:
A sensitive, minimally-perturbing fluorescence-resonance-energy-transfer biosensor (FynSensor) that reveals cellular Fyn activity to be spatially localized, pulsatile and sensitive to adhesion/integrin signaling and clarifies how compartmentalized Src-kinase activity may drive cell fate.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 27, 2020
2020 journal article
Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display
BIOCONJUGATE CHEMISTRY, 31(10), 2325–2338.
MeSH headings : Animals; Cell Line; Cell-Penetrating Peptides / chemistry; Cell-Penetrating Peptides / genetics; Cell-Penetrating Peptides / pharmacokinetics; Drosophila melanogaster / embryology; Gene Library; Humans; Mice; Models, Molecular; NIH 3T3 Cells; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / pharmacokinetics; Permeability; RNA, Messenger / genetics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 21, 2020
2020 journal article
Genetically encoded live-cell sensor for tyrosinated microtubules
JOURNAL OF CELL BIOLOGY, 219(10).
MeSH headings : Colchicine / pharmacology; Cytoskeleton / drug effects; Cytoskeleton / genetics; HEK293 Cells; Humans; Microtubule-Associated Proteins / genetics; Microtubules / genetics; Nocodazole / pharmacology; Protein Processing, Post-Translational / drug effects; Protein Processing, Post-Translational / genetics; Tubulin / genetics; Tyrosine / drug effects; Tyrosine / genetics; Vincristine / pharmacology
TL;DR:
The tyrosination sensor is reported, the first genetically encoded sensor against tyrosinated microtubules, which has potential applications to study microtubule and their PTMs in living cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: November 9, 2020
2020 journal article
Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display
ACS COMBINATORIAL SCIENCE, 22(10), 519–532.
author keywords: cyclic peptides; interleukin-17 (IL-17); library screening; yeast-display libraries; affinity ligands
MeSH headings : Biophysical Phenomena; Cell Membrane / chemistry; Cyclization; Flow Cytometry; Humans; Interleukin-17 / chemistry; Molecular Docking Simulation; Peptide Library; Peptides, Cyclic / isolation & purification; Protein Binding; Protein Conformation; Protein Engineering; Recombinant Proteins / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / ultrastructure; Structure-Activity Relationship; Surface Properties
TL;DR:
The results show that yeast surface display can be used to efficiently isolate and characterize cyclic peptides generated by chemical modification from combinatorial libraries.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020
2020 review
Past, Present, and Future of Affinity-based Cell Separation Technologies
[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.
author keywords: Cell purification; Immunoaffinity; MACS; FACS; Microfluidics
MeSH headings : Cell Separation; Chromatography, Affinity; Flow Cytometry; Ligands; Regenerative Medicine
TL;DR:
This review discusses past and current methods for developing cell-targeting affinity ligands and their application in cell purification, along with the benefits and challenges associated with different purification formats, and presents new technologies towards improving the viability and throughput of cell products for tissue engineering and regenerative medicine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 3, 2020
2020 article
Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping
GENOTYPE PHENOTYPE COUPLING: METHODS AND PROTOCOLS, Vol. 2070, pp. 321–334.
author keywords: Yeast surface display; Self-cleaving peptides; 2A peptides; Secretion; Ribosomal skipping; Sso7d
MeSH headings : Peptide Library; Peptides / metabolism; Protein Biosynthesis; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Ribosomes / metabolism; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
TL;DR:
This work has developed a platform that exploits inefficient ribosomal skipping by the F2A peptide for simultaneous soluble secretion and cell surface display of protein in the yeast Saccharomyces cerevisiae and provides specific recommendations for the purification of the secreted protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 7, 2020
2020 journal article
Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands
ACS COMBINATORIAL SCIENCE, 22(12), 738–744.
author keywords: yeast surface display; yeast magnetization; peptide ligands; mRNA-display library; ligand discovery
MeSH headings : Ligands; Mitochondrial Membrane Transport Proteins / chemistry; Models, Molecular; Peptide Library; RNA, Messenger / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae Proteins / chemistry; Transcription Factors / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021
2019 article
DERIVATION OF HUMAN TROPHOBLAST STEM CELLS FROM HUMAN PLURIPOTENT STEM CELLS
Mischler, A., Karakis, V., San Miguel, A., & Rao, B. (2019, August). PLACENTA, Vol. 83, pp. E59–E59.
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 20, 2020
2019 journal article
Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation
SCIENCE SIGNALING, 12(584).
MeSH headings : Animals; Biosensing Techniques; Cell Line; Endocytosis; ErbB Receptors / genetics; ErbB Receptors / metabolism; Mice; Microscopy, Fluorescence; Phospholipase C gamma / genetics; Phospholipase C gamma / metabolism; Phosphorylation / genetics; src Homology Domains
TL;DR:
The authors used two different mutagenesis and screening approaches to engineer new biosensors, mSH2 and SPY992, that exhibited greater specificity for EGFR Tyr992 and more accurately reported EGFR signaling kinetics, and could be extended to generate phospho-specific biosensor for various targets.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 24, 2019
2019 journal article
Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders
ACS COMBINATORIAL SCIENCE, 21(12), 817–832.
author keywords: yeast magnetization; combinatorial library screening; ligand discovery; yeast surface display; membrane protein; Sso7d; nanobody
MeSH headings : Combinatorial Chemistry Techniques; Drug Evaluation, Preclinical; Ferric Compounds / chemistry; Ferric Compounds / pharmacology; Magnetic Phenomena; Mitochondria / drug effects; Mitochondria / metabolism; Mitochondrial Membrane Transport Proteins / antagonists & inhibitors; Mitochondrial Membrane Transport Proteins / isolation & purification; Mitochondrial Membrane Transport Proteins / metabolism; Nanoparticles / chemistry; Protein Binding / drug effects; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / antagonists & inhibitors; Saccharomyces cerevisiae Proteins / isolation & purification; Saccharomyces cerevisiae Proteins / metabolism; Small Molecule Libraries / chemistry; Small Molecule Libraries / pharmacology
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 30, 2019
2018 journal article
An Engineered Sso7d Variant Enables Efficient Magnetization of Yeast Cells
ACS COMBINATORIAL SCIENCE, 20(10), 579–584.
author keywords: Sso7d; magnetization; cell capture; yeast surface display; magnetic separation
MeSH headings : Amino Acid Sequence; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Cell Membrane / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Magnetic Phenomena; Magnetite Nanoparticles / chemistry; Mutation; Peptide Library; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
TL;DR:
It is shown that cell surface expression of a mutant protein that binds iron oxide can enable efficient magnetization of yeast cells, which can enable a range of industrial and biotechnology applications, where easy separation of cells or organelles from complex media is desirable.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 29, 2018
2018 journal article
Kinetic Study of Degrafting Poly(methyl methacrylate) Brushes from Flat Substrates by Tetrabutylammonium Fluoride
MACROMOLECULES, 51(24), 10237–10245.
Sources: Web Of Science, NC State University Libraries
Added: January 14, 2019
2017 journal article
A defined and serum-free culture system for trophoblast differentiation of human embryonic stem cells
Placenta, 57, 237–238.
Source: Crossref
Added: February 24, 2020
2017 journal article
Design and Fabrication of Wettability Gradients with Tunable Profiles through Degrafting Organosilane Layers from Silica Surfaces by Tetrabutylammonium Fluoride
LANGMUIR, 33(51), 14556–14564.
TL;DR:
The fabrication of surface wettability gradients on flat surfaces is described by a simple, two-step procedure that permits precise tuning of the gradient profile and observes a relatively homogeneous coverage of silane throughout the degrafting process, providing a much more uniform surface when compared to additive approaches of gradient formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
IFPA meeting 2016 workshop report I: Genomic communication, bioinformatics, trophoblast biology and transport systems
Placenta, 60, S5–S9.
author keywords: Transport; Trophoblast; Bioinformatics; Exosomes; OMICS; DNA methylation
MeSH headings : Animals; Biological Transport; Biomedical Research / methods; Biomedical Research / trends; Computational Biology / methods; Computational Biology / trends; Congresses as Topic; DNA Methylation; Exome; Female; Genomics / methods; Genomics / trends; Humans; International Agencies; Maternal-Fetal Exchange; Placenta / cytology; Placenta / pathology; Placenta / physiology; Placenta / physiopathology; Pregnancy; Pregnancy Complications / pathology; Pregnancy Complications / physiopathology; Societies, Scientific; Trophoblasts / cytology; Trophoblasts / pathology; Trophoblasts / physiology
TL;DR:
These workshops covered innovative technologies applied to new and traditional areas of placental research: 1) genomic communication; 2) bioinformatics; 3) trophoblast biology and pathology; 4) placental transport systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
5. Gender Equality
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 6, 2018
2017 journal article
Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
SCIENTIFIC REPORTS, 7.
MeSH headings : Animals; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Binding, Competitive / drug effects; Carrier Proteins / genetics; Carrier Proteins / metabolism; Carrier Proteins / pharmacology; Cell Line, Tumor; Cell Membrane / drug effects; Cell Membrane / metabolism; Colorectal Neoplasms / metabolism; Colorectal Neoplasms / pathology; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; HCT116 Cells; HEK293 Cells; Humans; MCF-7 Cells; Protein Binding; RNA Interference; Receptor, Notch1 / antagonists & inhibitors; Receptor, Notch1 / genetics; Receptor, Notch1 / metabolism; Spheroids, Cellular / drug effects; Spheroids, Cellular / metabolism
TL;DR:
The isolation and characterization of a novel Notch1-binding protein, N9, obtained by screening of a combinatorial library based on the ultra-stable Sso7d scaffold is described, suggesting its potential as a therapeutic agent in Notch-associated malignancies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae
ACS SYNTHETIC BIOLOGY, 6(11), 2096–2107.
author keywords: yeast surface display; secretion; ribosomal skipping; 2A peptide; Sso7d; glucose oxidase
MeSH headings : Cell Adhesion Molecules / genetics; Cell Adhesion Molecules / metabolism; Gene Expression; Peptide Library; Peptides / genetics; Peptides / metabolism; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism
TL;DR:
A system for simultaneous cell surface display and soluble secretion of proteins in Saccharomyces cerevisiae based on inefficient ribosomal skipping is described and it is shown that binding proteins derived from the Sso7d scaffold and the homodimeric enzyme glucose oxidase can be simultaneously secreted solubly and displayed on the cell surface.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
BMP signaling plays a biphasic role during trophoblast differentiation of human embryonic stem cells
Placenta, 45, 99–100.
Source: Crossref
Added: February 24, 2020
2016 journal article
Combinatorial Pairwise Assembly Efficiently Generates High Affinity Binders and Enables a "Mix-and-Read" Detection Scheme
ACS SYNTHETIC BIOLOGY, 5(12), 1348–1354.
author keywords: Sso7d; binding affinity; yeast surface display; flexible linker; split GFP
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Mutagenesis; Peptide Library; Plasmids / genetics; Protein Conformation; Protein Engineering; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Yeasts / genetics; Yeasts / metabolism
TL;DR:
It is shown that a combinatorial library constructed by random pairwise assembly of low affinity binders can efficiently generate binders with increased affinity, and such a library based on the Sso7d scaffold contained putative high affinity clones for a model target at higher frequency than a library of monovalent mutants generated by random mutagenesis alone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts
JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.
author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
TL;DR:
The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins
LANGMUIR, 32(34), 8660–8667.
TL;DR:
Results strongly suggest that mutations introduced in Sso7d-2B5 alter its orientation relative to the parent protein, when adsorbed on silica surfaces, and has direct relevance to development of protein-based biosensors and biocatalysts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts
JOURNAL OF BIOLOGICAL CHEMISTRY, 290(14), 8834–8848.
MeSH headings : Activins / metabolism; Base Sequence; Cell Differentiation; Cell Lineage; DNA Methylation; DNA Primers; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Ephrin-B2 / genetics; Humans; Nodal Protein / metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Signal Transduction; Trophoblasts / cytology; Trophoblasts / metabolism
TL;DR:
It is shown that activin/nodal signaling switches the terminal fate of these hESC-derived trophoblasts, providing a model system that allows for targeted derivation of extravillous trophoblast and syncytiotrophoblasts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Avidity-mediated virus separation using a hyperthermophilic affinity ligand
BIOTECHNOLOGY PROGRESS, 29(1), 237–246.
author keywords: avidity; affinity ligand; hyperthermophilic; multivalent
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Ligands; Molecular Weight; Plant Extracts; Plant Leaves / virology; Sulfolobus solfataricus / chemistry; Sulfolobus solfataricus / metabolism; Tobacco / virology; Tombusviridae / isolation & purification
TL;DR:
The approach may be broadly relevant to cases where capture of large multivalent species from complex mixtures and subsequent release without the use of harsh elution conditions is necessary, and bypasses chemical conjugation of the affinity ligand to the capture surface.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Generation and Properties of Antibacterial Coatings Based on Electrostatic Attachment of Silver Nanoparticles to Protein-Coated Polypropylene Fibers
ACS APPLIED MATERIALS & INTERFACES, 5(11), 5298–5306.
author keywords: antibacterial; polypropylene; protein adsorption; nanoparticle; functional coating
MeSH headings : Anti-Bacterial Agents / chemistry; Coated Materials, Biocompatible / chemistry; Escherichia coli / drug effects; Escherichia coli Infections / prevention & control; Hydrophobic and Hydrophilic Interactions; Light; Metal Nanoparticles / chemistry; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Polypropylenes / chemistry; Proteins / chemistry; Silver / chemistry; Static Electricity; Ultraviolet Rays
TL;DR:
The results show that PP surfaces coated with AgNPs exhibit excellent antibacterial activity with 100% removal efficiency and are tested against gram negative bacteria Escherichia coli.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast
ACS APPLIED MATERIALS & INTERFACES, 5(19), 9349–9354.
author keywords: electrospinning; nanofiber; protein; biocatalyst immobilization; yeast surface display
MeSH headings : Biocatalysis; Cell Survival / drug effects; Green Fluorescent Proteins; Microscopy, Electron, Scanning; Nanofibers / chemistry; Polymers / chemistry; Polymers / pharmacology; Polyvinyl Alcohol / chemistry; Polyvinyl Alcohol / pharmacology; Protein Conformation / drug effects; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / growth & development; Water / chemistry
TL;DR:
This method of processing functionalized yeast may be a powerful tool in the direct immobilization of properly folded, active enzymes within electrospun nanofibers with potential applications in biocatalysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 review
Protein selection using yeast surface display
[Review of ]. METHODS, 60(1), 15–26.
author keywords: Yeast surface display; Protein engineering; Library screening; Biophysical characterization; Alternative scaffolds
MeSH headings : Combinatorial Chemistry Techniques; Flow Cytometry; Protein Engineering / methods; Yeasts / genetics; Yeasts / metabolism
TL;DR:
This review presents detailed protocols for the use of yeast surface display to isolate de novo binding proteins from combinatorial libraries, and subsequent biophysical characterization of binders.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Scaffold Diversification Enhances Effectiveness of a Super library of Hyperthermophilic Proteins
ACS SYNTHETIC BIOLOGY, 2(1), 6–13.
author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; alternate scaffold; mRNA display
MeSH headings : Biotechnology / methods; Carrier Proteins / genetics; Carrier Proteins / metabolism; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Models, Molecular; Peptide Library; Protein Conformation; Yeasts / genetics; Yeasts / metabolism
TL;DR:
Scaffold diversification, i.e., randomization of multiple different scaffolds, is a powerful alternate strategy for combinatorial library construction and binders from this modestly sized superlibrary have affinities comparable or higher than those obtained from a library with 1000-fold higher sequence diversity but derived from a single stable scaffold.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 review
Trophoblast differentiation of human embryonic stem cells
[Review of ]. BIOTECHNOLOGY JOURNAL, 8(4), 421-+.
author keywords: Bone morphogenetic protein; CDX2; ELF5; hESC; Trophoblast
MeSH headings : Animals; Bone Morphogenetic Proteins / pharmacology; Cell Culture Techniques; Cell Differentiation / drug effects; Cell Differentiation / physiology; Embryonic Stem Cells / cytology; Embryonic Stem Cells / drug effects; Humans; Trophoblasts / cytology; Trophoblasts / drug effects
TL;DR:
An overview of previously described efforts to obtain trophoblasts from hESCs is provided and the merits and limitations of hESC‐derived cells as a source of trophoblast derivatives are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands
Biotechnology and Bioengineering, 110(3), 857–870.
author keywords: cyclic peptide; mRNA display; Fc binding; affinity ligand; library
MeSH headings : Humans; Immunoglobulin Fc Fragments / metabolism; Peptide Library; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / metabolism; Protein Binding; Protein Engineering / methods; RNA, Messenger / genetics; Solid-Phase Synthesis Techniques / methods
TL;DR:
The discovery, synthesis and characterization of novel cyclic peptide affinity ligands that bind the Fc portion of human Immunoglobulin G (IgG; hFc) are described and the approach can be extended to efficiently generate and evaluatecyclic peptides for other targets of interest.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold
PLOS ONE, 7(11).
MeSH headings : Carrier Proteins / genetics; Carrier Proteins / metabolism; Epitopes / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Histidine / genetics; Histidine / metabolism; Humans; Hydrogen-Ion Concentration; Immunoglobulin Fc Fragments / genetics; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / genetics; Immunoglobulin G / metabolism; Mutagenesis; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism
TL;DR:
P pH sensitive hFc binders are attractive candidates for use in chromatography, to achieve elution of IgG under milder pH conditions, but further studies are needed to evaluate if the Sso7d mutants identified in this study are indeed useful as affinity ligands in Chromatography.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells
MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839.
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned / analysis; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Mice; Proteins / analysis; Secretory Pathway; Subcellular Fractions / chemistry
TL;DR:
Targeted proteomics of secretory pathway organelles is shown to be a powerful alternate approach for interrogating the cellular secretome and expected to be particularly valuable in two contexts highly relevant to hESC biology: obtaining a temporal snapshot of proteins secreted in response to a differentiation trigger, and identifying proteinssecreted by cells that are isolated from a heterogeneous population.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
The subcellular proteome of undifferentiated human embryonic stem cells
PROTEOMICS, 12(3), 421–430.
author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
TL;DR:
The results show that systematic subcellular proteome analysis is a valuable tool for studying hESC biology and shows that proteomic analysis of the endoplasmic reticulum and Golgi compartments is a powerful alternative approach to identify secreted proteins since these are synthesized in the ER and transit through the Golgi.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.
MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
TL;DR:
The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold
JOURNAL OF MOLECULAR BIOLOGY, 409(4), 601–616.
author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; stability; alternate scaffold
MeSH headings : Amino Acid Sequence; Animals; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Chickens; DNA Mutational Analysis; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Fluorescein / chemistry; Fluorescein / metabolism; Immunoglobulins / chemistry; Immunoglobulins / metabolism; Mice; Molecular Sequence Data; Muramidase / chemistry; Muramidase / metabolism; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protein Denaturation; Streptavidin / chemistry; Streptavidin / metabolism; Sulfolobus solfataricus / chemistry; beta Catenin / chemistry; beta Catenin / metabolism
TL;DR:
It is shown that highly stable binding proteins for a wide spectrum of targets can be generated through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus, and that S so7d-derived binding proteins could discriminate between closely related immunoglobulins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Role of Signaling Pathways and Epigenetic Factors in Lineage Determination During Human Embryonic Stem Cell Differentiation
Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, 1, 1.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics
ANALYTICAL CHEMISTRY, 82(20), 8696–8702.
MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
TL;DR:
A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Quantitative top-down proteomics of SILAC labeled human embryonic stem cells
Journal of the American Society for Mass Spectrometry, 21(6), 879–889.
MeSH headings : Amino Acid Sequence; Animals; Arginine / chemistry; Arginine / metabolism; Carbon Isotopes; Cell Line; Embryonic Stem Cells / chemistry; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Lysine / chemistry; Lysine / metabolism; Mass Spectrometry / methods; Mice; Proteome / analysis; Proteomics / methods
TL;DR:
This study has confidently identified 11 proteins by accurate intact mass, MS/MS, and amino acid counting facilitated by SILAC labeling, which to the best of the authors' knowledge represents the first top-down analysis of hESCs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2009 journal article
Molecular aspects of cardiac differentiation in embryonic stem cells
Critical Reviews in Biomedical Engineering, 37(4-5), 283–320.
MeSH headings : Animals; Cell Differentiation / physiology; Cells, Cultured; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Models, Biological; Myocytes, Cardiac / cytology; Myocytes, Cardiac / physiology; Signal Transduction / physiology; Transcription Factors / metabolism
TL;DR:
The current methods to obtain cells of the cardiac lineages from ESCs are summarized and a review of the signaling pathways, transcription factors, and target genes involved in cardiac differentiation are presented.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
2008 journal article
TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal
NATURE CELL BIOLOGY, 10(7), 837–848.
MeSH headings : Active Transport, Cell Nucleus / physiology; Acyltransferases; Animals; Cell Line; Cell Nucleus / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / physiology; Genes, Reporter; Humans; Mediator Complex; RNA, Small Interfering / genetics; RNA, Small Interfering / metabolism; Signal Transduction / physiology; Smad2 Protein / genetics; Smad2 Protein / metabolism; Smad3 Protein / genetics; Smad3 Protein / metabolism; Smad4 Protein / genetics; Smad4 Protein / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic; Transforming Growth Factor beta / metabolism
TL;DR:
It is demonstrated that in response to TGFβ stimulation the transcriptional regulator TAZ binds heteromeric Smad2/3–4 complexes and is recruited to T GFβ response elements and defines a hierarchical system regulating Smad nuclear accumulation and coupling to the transcriptionAL machinery.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Niche-mediated control of human embryonic stem cell self-renewal and differentiation
EMBO JOURNAL, 26(22), 4744–4755.
author keywords: embryonic stem cell; micropatterning; niche; self-renewal; Smad signaling
MeSH headings : Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins / metabolism; Cell Differentiation; Cell Line; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoderm / metabolism; Fibroblast Growth Factors / antagonists & inhibitors; Growth Differentiation Factor 3; Humans; Smad1 Protein / metabolism; Transforming Growth Factor beta / antagonists & inhibitors; Transforming Growth Factor beta / metabolism
TL;DR:
It is demonstrated that the rate and trajectory of hESC differentiation can be controlled by engineering hESCs niche properties and a role for Smad1 is illustrated, for the first time, in the integration of spatial information and in the niche‐size‐dependent control of h ESC self‐renewal and differentiation.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Improved mutants from directed evolution are biased to orthologous substitutions
PROTEIN ENGINEERING DESIGN & SELECTION, 19(6), 245–253.
author keywords: epidermal growth factor; epidermal growth factor receptor; in vitro evolution; protein engineering; yeast display
MeSH headings : Base Sequence; Biosensing Techniques; Directed Molecular Evolution; Epidermal Growth Factor / chemistry; Epidermal Growth Factor / genetics; Epidermal Growth Factor / metabolism; ErbB Receptors / genetics; ErbB Receptors / metabolism; Fibroblasts / metabolism; Gene Library; Humans; Interleukin-2 / genetics; Interleukin-2 / metabolism; Mutagenesis; Mutation; Phylogeny; Protein Binding; Subtilisins / genetics; Subtilisins / metabolism; Transfection / methods; Yeasts / genetics
TL;DR:
These findings highlight a general approach for harnessing information present in phylogenetic variability to create useful genetic diversity for directed evolution and exploits the benefits of library diversity obtained through complementary methods of error-prone PCR and DNA shuffling while circumventing the need for acquisition of multiple genes for family or synthetic shuffling.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2005 review
Culture development for human embryonic stem cell propagation: molecular aspects and challenges
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 16(5), 568–576.
TL;DR:
Basic fibroblast growth factor and members of the transforming growth factor-beta superfamily are important regulators of human embryonic stem cell self-renewal and extensive cross-talk between the intracellular signaling pathways activated by these factors contributes to maintenance of the undifferentiated hESC state.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Engineering protein therapeutics within a systems-level computational context
Nature Biotechnology, 23(2), 191–194.
Source: NC State University Libraries
Added: August 6, 2018
2005 journal article
High-Affinity CD25-Binding IL-2 Mutants Potently Stimulate Persistent T Cell Growth†
Biochemistry, 44(31), 10696–10701.
MeSH headings : Amino Acid Substitution / genetics; Cell Line; Cell Membrane / genetics; Cell Membrane / immunology; Cell Membrane / metabolism; Cell Proliferation; Directed Molecular Evolution; Humans; Interleukin-15 / metabolism; Interleukin-15 / physiology; Interleukin-2 / genetics; Interleukin-2 / metabolism; Interleukin-2 / physiology; Interleukin-2 Receptor alpha Subunit; Lymphocyte Activation / genetics; Mitogens / genetics; Mitogens / metabolism; Mitogens / physiology; Protein Binding / genetics; Receptors, Interleukin / metabolism; Receptors, Interleukin-15; Receptors, Interleukin-2 / metabolism; T-Lymphocytes / cytology; T-Lymphocytes / immunology
TL;DR:
These IL-2 mutants exhibit T cell growth response-receptor occupancy curves indistinguishable from that for IL-15, suggesting that much of the difference between wild-type IL-1 and IL-3 effects arises simply from their 1000-fold differing affinities for their private alpha receptor subunits.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2005 journal article
Integrating cell-level kinetic modeling into the design of engineered protein therapeutics
NATURE BIOTECHNOLOGY, 23(2), 191–194.
MeSH headings : Cell Physiological Phenomena; Computer Simulation; Drug Delivery Systems / methods; Drug Design; Drug Therapy, Computer-Assisted / methods; Gene Expression Regulation / drug effects; Gene Expression Regulation / physiology; Kinetics; Models, Biological; Protein Engineering / methods
TL;DR:
This work states thatFunctional genomics and proteomics are identifying many potential drug targets for novel therapeutic proteins, and both rational and combinatorial protein engineering methods are available for creating drug candidates.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2004 journal article
Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect
Molecular Pharmacology, 66(4), 864–869.
Source: NC State University Libraries
Added: August 6, 2018
2003 journal article
Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity
PROTEIN ENGINEERING, 16(12), 1081–1087.
author keywords: alpha-receptor subunit binding affinity; interleukin-2 mutants; T-cell stimulation
MeSH headings : Cell Division / genetics; Cell Division / physiology; Cloning, Molecular; Interleukin-2 / genetics; Interleukin-2 / metabolism; Interleukin-2 Receptor alpha Subunit; Mutation; Protein Binding / genetics; Protein Binding / physiology; Receptors, Interleukin / metabolism; Saccharomyces cerevisiae
TL;DR:
A mutant was serendipitously identified that exhibited somewhat improved potency, perhaps via altered endocytic trafficking mechanisms described previously, and this mutant was screened libraries of mutated IL-2 displayed on the surface of yeast and isolated mutants with a 15-30-fold improved affinity for theIL-2Ralpha subunit.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
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