Works (54)

2022 article

Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase

McArthur, N., Cruz-Teran, C., Thatavarty, A., Reeves, G. T., & Rao, B. M. (2022, July 1). ACS OMEGA.

By: N. McArthur n, C. Cruz-Teran n, A. Thatavarty n, G. Reeves & B. Rao

Source: Web Of Science
Added: July 26, 2022

2022 journal article

Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase

ACS OMEGA, 7(28), 24551–24560.

By: N. McArthur, C. Cruz-Teran, A. Thatavarty, G. Reeves & B. Rao

Source: Web Of Science
Added: August 8, 2022

2022 journal article

Modified Histone Peptides Linked to Magnetic Beads Reduce Binding Specificity

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(3).

By: J. Meanor, A. Keung & B. Rao

author keywords: yeast surface display; histone post-translational modifications; affinity reagents; protein engineering; chromodomain; binder; reader; antibody; epigenome engineering; synthetic biology
Source: Web Of Science
Added: February 21, 2022

2021 journal article

Mapping the residue specificities of epigenome enzymes by yeast surface display

CELL CHEMICAL BIOLOGY, 28(12), 1772-+.

By: A. Waldman, B. Rao & A. Keung

Source: Web Of Science
Added: January 3, 2022

2021 journal article

Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions

ACS SYNTHETIC BIOLOGY, 10(3), 505–514.

By: K. Bacon, A. Blain n, J. Bowen n, M. Burroughs n, N. McArthur n, S. Menegatti, B. Rao

author keywords: yeast two-hybrid; protein-protein interactions; protein engineering; yeast surface display; post-translational modification; cDNA library
Sources: Web Of Science, ORCID
Added: March 29, 2021

2021 journal article

Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4).

By: J. Bowen n, J. Schneible, K. Bacon, C. Labar n, S. Menegatti & B. Rao

author keywords: yeast display library; cyclic peptide; Yes Associated Protein (YAP) 65; WW domain; transglutaminase
Sources: Web Of Science, ORCID
Added: March 22, 2021

2021 journal article

Two distinct trophectoderm lineage stem cells from human pluripotent stem cells

JOURNAL OF BIOLOGICAL CHEMISTRY, 296.

Source: Web Of Science
Added: September 7, 2021

2020 journal article

A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells

ELIFE, 9.

By: A. Mukherjee*, R. Singh*, S. Udayan*, S. Biswas*, P. Reddy*, S. Manmadhan*, G. George*, S. Kumar* ...

Source: Web Of Science
Added: February 27, 2020

2020 journal article

Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display

BIOCONJUGATE CHEMISTRY, 31(10), 2325–2338.

By: J. Bowen n, A. Schloop, G. Reeves, S. Menegatti & B. Rao

Sources: Web Of Science, ORCID
Added: December 21, 2020

2020 journal article

Genetically encoded live-cell sensor for tyrosinated microtubules

JOURNAL OF CELL BIOLOGY, 219(10).

By: S. Kesarwani*, P. Lama*, A. Chandra*, P. Reddy*, A. Jijumon*, S. Bodakuntla*, B. Rao, C. Janke*, R. Das*, M. Sirajuddin

Source: Web Of Science
Added: November 9, 2020

2020 journal article

Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display

ACS COMBINATORIAL SCIENCE, 22(10), 519–532.

By: K. Bacon, A. Blain n, M. Burroughs n, N. McArthrur n, B. Rao & S. Menegatti

author keywords: cyclic peptides; interleukin-17 (IL-17); library screening; yeast-display libraries; affinity ligands
Sources: Web Of Science, ORCID
Added: November 9, 2020

2020 review

Past, Present, and Future of Affinity-based Cell Separation Technologies

[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.

author keywords: Cell purification; Immunoaffinity; MACS; FACS; Microfluidics
Sources: Web Of Science, ORCID
Added: August 3, 2020

2020 article

Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping

GENOTYPE PHENOTYPE COUPLING: METHODS AND PROTOCOLS, Vol. 2070, pp. 321–334.

By: C. Cruz-Teran n, K. Bacon & B. Rao

author keywords: Yeast surface display; Self-cleaving peptides; 2A peptides; Secretion; Ribosomal skipping; Sso7d
Source: Web Of Science
Added: September 7, 2020

2020 journal article

Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands

ACS COMBINATORIAL SCIENCE, 22(12), 738–744.

By: K. Bacon, J. Bowen n, H. Reese, B. Rao & S. Menegatti

author keywords: yeast surface display; yeast magnetization; peptide ligands; mRNA-display library; ligand discovery
Sources: Web Of Science, ORCID
Added: January 11, 2021

2019 article

DERIVATION OF HUMAN TROPHOBLAST STEM CELLS FROM HUMAN PLURIPOTENT STEM CELLS

Mischler, A., Karakis, V., San Miguel, A., & Rao, B. (2019, August). PLACENTA, Vol. 83, pp. E59–E59.

Source: Web Of Science
Added: April 20, 2020

2019 journal article

Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation

SCIENCE SIGNALING, 12(584).

Sources: Web Of Science, ORCID
Added: June 24, 2019

2019 journal article

Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders

ACS COMBINATORIAL SCIENCE, 21(12), 817–832.

By: K. Bacon, M. Burroughs, A. Blain, S. Menegatti & B. Rao

author keywords: yeast magnetization; combinatorial library screening; ligand discovery; yeast surface display; membrane protein; Sso7d; nanobody
Sources: Web Of Science, ORCID
Added: December 30, 2019

2018 journal article

An Engineered Sso7d Variant Enables Efficient Magnetization of Yeast Cells

ACS COMBINATORIAL SCIENCE, 20(10), 579–584.

By: C. Cruz-Teran n, K. Bacon n, N. McArthur n & B. Rao

author keywords: Sso7d; magnetization; cell capture; yeast surface display; magnetic separation
Source: Web Of Science
Added: October 29, 2018

2018 journal article

Kinetic Study of Degrafting Poly(methyl methacrylate) Brushes from Flat Substrates by Tetrabutylammonium Fluoride

MACROMOLECULES, 51(24), 10237–10245.

Sources: Web Of Science, ORCID
Added: January 14, 2019

2017 journal article

A defined and serum-free culture system for trophoblast differentiation of human embryonic stem cells

Placenta, 57, 237–238.

By: A. Mischler & B. Rao

Source: Crossref
Added: February 24, 2020

2017 journal article

Design and Fabrication of Wettability Gradients with Tunable Profiles through Degrafting Organosilane Layers from Silica Surfaces by Tetrabutylammonium Fluoride

LANGMUIR, 33(51), 14556–14564.

By: J. Miles, S. Schlenker n, Y. Ko, R. Patil n, B. Rao & J. Genzer

Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

IFPA meeting 2016 workshop report I: Genomic communication, bioinformatics, trophoblast biology and transport systems

Placenta, 60, S5–S9.

author keywords: Transport; Trophoblast; Bioinformatics; Exosomes; OMICS; DNA methylation
Sources: NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Identification and characterization of a novel Sso7d scaffold-based binder against Notch1

SCIENTIFIC REPORTS, 7.

By: T. Gocha*, B. Rao & R. DasGupta*

Source: Web Of Science
Added: August 6, 2018

2017 journal article

Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae

ACS SYNTHETIC BIOLOGY, 6(11), 2096–2107.

By: C. Cruz-Teran n, K. Tiruthani, A. Mischler & B. Rao

author keywords: yeast surface display; secretion; ribosomal skipping; 2A peptide; Sso7d; glucose oxidase
Source: Web Of Science
Added: August 6, 2018

2016 journal article

BMP signaling plays a biphasic role during trophoblast differentiation of human embryonic stem cells

Placenta, 45, 99–100.

By: P. Sarkar n, A. Mischler n & B. Rao

Source: Crossref
Added: February 24, 2020

2016 journal article

Combinatorial Pairwise Assembly Efficiently Generates High Affinity Binders and Enables a "Mix-and-Read" Detection Scheme

ACS SYNTHETIC BIOLOGY, 5(12), 1348–1354.

By: K. Carlin n, C. Cruz-Teran n, J. Kumar*, C. Gomes n & B. Rao

author keywords: Sso7d; binding affinity; yeast surface display; flexible linker; split GFP
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.

By: P. Sarkar, A. Mischler, S. Randall, T. Collier, K. Dorman*, K. Boggess*, D. Muddiman, B. Rao

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins

LANGMUIR, 32(34), 8660–8667.

By: C. Cruz-Teran n, K. Carlin n, K. Efimenko, J. Genzer & B. Rao

Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(14), 8834–8848.

By: P. Sarkar, S. Randall*, T. Collier*, A. Nero*, T. Russell n, D. Muddiman, B. Rao

Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Avidity-mediated virus separation using a hyperthermophilic affinity ligand

BIOTECHNOLOGY PROGRESS, 29(1), 237–246.

author keywords: avidity; affinity ligand; hyperthermophilic; multivalent
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Generation and Properties of Antibacterial Coatings Based on Electrostatic Attachment of Silver Nanoparticles to Protein-Coated Polypropylene Fibers

ACS APPLIED MATERIALS & INTERFACES, 5(11), 5298–5306.

By: K. Goli, N. Gera, X. Liu, B. Rao, O. Rojas & J. Genzer

author keywords: antibacterial; polypropylene; protein adsorption; nanoparticle; functional coating
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast

ACS APPLIED MATERIALS & INTERFACES, 5(19), 9349–9354.

By: M. Canbolat n, N. Gera, C. Tang, B. Monian n, B. Rao, B. Pourdeyhimi, S. Khan

author keywords: electrospinning; nanofiber; protein; biocatalyst immobilization; yeast surface display
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 review

Protein selection using yeast surface display

[Review of ]. METHODS, 60(1), 15–26.

By: N. Gera, M. Hussain & B. Rao

author keywords: Yeast surface display; Protein engineering; Library screening; Biophysical characterization; Alternative scaffolds
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Scaffold Diversification Enhances Effectiveness of a Super library of Hyperthermophilic Proteins

ACS SYNTHETIC BIOLOGY, 2(1), 6–13.

author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; alternate scaffold; mRNA display
Source: Web Of Science
Added: August 6, 2018

2013 review

Trophoblast differentiation of human embryonic stem cells

[Review of ]. BIOTECHNOLOGY JOURNAL, 8(4), 421-+.

author keywords: Bone morphogenetic protein; CDX2; ELF5; hESC; Trophoblast
Source: Web Of Science
Added: August 6, 2018

2013 journal article

mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands

Biotechnology and Bioengineering, 110(3), 857–870.

author keywords: cyclic peptide; mRNA display; Fc binding; affinity ligand; library
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold

PLOS ONE, 7(11).

Source: Web Of Science
Added: August 6, 2018

2012 journal article

Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells

MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839.

By: P. Sarkar, S. Randall, D. Muddiman & B. Rao

Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

The subcellular proteome of undifferentiated human embryonic stem cells

PROTEOMICS, 12(3), 421–430.

author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.

Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold

JOURNAL OF MOLECULAR BIOLOGY, 409(4), 601–616.

author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; stability; alternate scaffold
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Role of Signaling Pathways and Epigenetic Factors in Lineage Determination During Human Embryonic Stem Cell Differentiation

Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, 1, 1.

By: P. Sarkar & B. Rao

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics

ANALYTICAL CHEMISTRY, 82(20), 8696–8702.

By: T. Collier, P. Sarkar n, W. Franck, B. Rao, R. Dean & D. Muddiman

Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Quantitative top-down proteomics of SILAC labeled human embryonic stem cells

Journal of the American Society for Mass Spectrometry, 21(6), 879–889.

By: T. Collier, P. Sarkar n, B. Rao & D. Muddiman

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2009 journal article

Molecular aspects of cardiac differentiation in embryonic stem cells

Critical Reviews in Biomedical Engineering, 37(4-5), 283–320.

By: P. Sarkar n & B. Rao

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal

NATURE CELL BIOLOGY, 10(7), 837–848.

By: X. Varelas*, R. Sakuma*, P. Samavarchi-Tehrani*, R. Peerani*, B. Rao, J. Dembowy*, M. Yaffe*, P. Zandstra*, J. Wrana*

Source: Web Of Science
Added: August 6, 2018

2007 journal article

Niche-mediated control of human embryonic stem cell self-renewal and differentiation

EMBO JOURNAL, 26(22), 4744–4755.

By: R. Peerani*, B. Rao, C. Bauwens*, T. Yin*, G. Wood*, A. Nagy*, E. Kumacheva*, P. Zandstra*

author keywords: embryonic stem cell; micropatterning; niche; self-renewal; Smad signaling
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Improved mutants from directed evolution are biased to orthologous substitutions

PROTEIN ENGINEERING DESIGN & SELECTION, 19(6), 245–253.

By: J. Cochran*, Y. Kim*, S. Lippow*, B. Rao & K. Wittrup*

author keywords: epidermal growth factor; epidermal growth factor receptor; in vitro evolution; protein engineering; yeast display
Source: Web Of Science
Added: August 6, 2018

2005 review

Culture development for human embryonic stem cell propagation: molecular aspects and challenges

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 16(5), 568–576.

By: B. Rao & P. Zandstra*

Source: Web Of Science
Added: August 6, 2018

2005 journal article

Engineering protein therapeutics within a systems-level computational context

Nature Biotechnology, 23(2), 191–194.

By: B. Rao, D. Lauffenburger & K. Wittrup

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

High-Affinity CD25-Binding IL-2 Mutants Potently Stimulate Persistent T Cell Growth†

Biochemistry, 44(31), 10696–10701.

Source: Crossref
Added: August 28, 2020

2005 journal article

Integrating cell-level kinetic modeling into the design of engineered protein therapeutics

NATURE BIOTECHNOLOGY, 23(2), 191–194.

Source: Web Of Science
Added: August 6, 2018

2004 journal article

Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect

Molecular Pharmacology, 66(4), 864–869.

By: B. Rao, I. Driver, D. Lauffenburger & K. Wittrup

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity

PROTEIN ENGINEERING, 16(12), 1081–1087.

By: B. Rao, A. Girvin, T. Ciardelli, D. Lauffenburger & K. Wittrup

author keywords: alpha-receptor subunit binding affinity; interleukin-2 mutants; T-cell stimulation
Source: Web Of Science
Added: August 6, 2018