Works (55)

Updated: April 3rd, 2024 21:00

2023 journal article

Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions

JOURNAL OF BIOLOGICAL CHEMISTRY, 299(5).

By: V. Karakis n, M. Jabeen n, J. Britt n, A. Cordiner n, A. Mischler n, F. Li*, A. Miguel n, B. Rao n

MeSH headings : Humans; Cell Differentiation / drug effects; Colforsin / pharmacology; Colforsin / metabolism; HLA-G Antigens / genetics; HLA-G Antigens / metabolism; Laminin / pharmacology; Stem Cells / cytology; Stem Cells / drug effects; Transforming Growth Factor beta / metabolism; Trophoblasts / cytology; Trophoblasts / metabolism; Culture Media / chemistry; Hypoxia-Inducible Factor 1, alpha Subunit / genetics; Gene Expression Regulation, Developmental / drug effects; Cytological Techniques / methods
Sources: Web Of Science, ORCID
Added: July 3, 2023

2022 article

Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase

McArthur, N., Cruz-Teran, C., Thatavarty, A., Reeves, G. T., & Rao, B. M. (2022, July 1). ACS OMEGA.

By: N. McArthur n, C. Cruz-Teran n, A. Thatavarty n, G. Reeves* & B. Rao n

Source: Web Of Science
Added: July 26, 2022

2022 journal article

Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase

ACS OMEGA, 7(28), 24551–24560.

By: N. McArthur, C. Cruz-Teran, A. Thatavarty, G. Reeves & B. Rao

Source: Web Of Science
Added: August 8, 2022

2022 journal article

Modified Histone Peptides Linked to Magnetic Beads Reduce Binding Specificity

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(3).

By: J. Meanor n, A. Keung n & B. Rao n

author keywords: yeast surface display; histone post-translational modifications; affinity reagents; protein engineering; chromodomain; binder; reader; antibody; epigenome engineering; synthetic biology
MeSH headings : HEK293 Cells; Histone Code; Histones / metabolism; Humans; Jurkat Cells; K562 Cells; Protein Array Analysis; Protein Binding; Protein Engineering; Protein Processing, Post-Translational; Saccharomyces cerevisiae
Source: Web Of Science
Added: February 21, 2022

2021 journal article

Mapping the residue specificities of epigenome enzymes by yeast surface display

CELL CHEMICAL BIOLOGY, 28(12), 1772-+.

By: A. Waldman n, B. Rao n & A. Keung n

MeSH headings : E1A-Associated p300 Protein / genetics; E1A-Associated p300 Protein / metabolism; Epigenome / genetics; Humans; Saccharomyces cerevisiae / metabolism
Source: Web Of Science
Added: January 3, 2022

2021 journal article

Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions

ACS SYNTHETIC BIOLOGY, 10(3), 505–514.

By: K. Bacon n, A. Blain n, J. Bowen n, M. Burroughs n, N. McArthur n, S. Menegatti n, B. Rao n

author keywords: yeast two-hybrid; protein-protein interactions; protein engineering; yeast surface display; post-translational modification; cDNA library
MeSH headings : Gene Library; Genes, Reporter; Protein Binding; Protein Domains; Protein Interaction Maps; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Smad3 Protein / chemistry; Smad3 Protein / metabolism; Transcription Factors / chemistry; Transcription Factors / metabolism; Two-Hybrid System Techniques
Sources: Web Of Science, ORCID
Added: March 29, 2021

2021 journal article

Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4).

By: J. Bowen n, J. Schneible n, K. Bacon n, C. Labar n, S. Menegatti n & B. Rao n

author keywords: yeast display library; cyclic peptide; Yes Associated Protein (YAP) 65; WW domain; transglutaminase
MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Adaptor Proteins, Signal Transducing / metabolism; Albumins / metabolism; Binding Sites; Combinatorial Chemistry Techniques; Endoplasmic Reticulum / metabolism; Flow Cytometry; Ligands; Muramidase / metabolism; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / pharmacology; Protein Binding; Protein Engineering / methods; Transcription Factors / chemistry; Transcription Factors / metabolism; Transglutaminases / metabolism; YAP-Signaling Proteins; Yeasts / genetics; Yeasts / growth & development
Sources: Web Of Science, ORCID
Added: March 22, 2021

2021 journal article

Two distinct trophectoderm lineage stem cells from human pluripotent stem cells

JOURNAL OF BIOLOGICAL CHEMISTRY, 296.

By: A. Mischler n, V. Karakis n, J. Mahinthakumar n, C. Carberry*, A. San Miguel n, J. Rager*, R. Fry*, B. Rao n

MeSH headings : CDX2 Transcription Factor / metabolism; Cell Culture Techniques; Cell Differentiation; Cell Lineage; Culture Media; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Female; Humans; Placenta / cytology; Placenta / metabolism; Pluripotent Stem Cells / cytology; Pluripotent Stem Cells / metabolism; Pregnancy; Trophoblasts / cytology; Trophoblasts / metabolism
Sources: Web Of Science, ORCID
Added: September 7, 2021

2020 journal article

A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells

ELIFE, 9.

By: A. Mukherjee*, R. Singh*, S. Udayan*, S. Biswas*, P. Reddy*, S. Manmadhan*, G. George*, S. Kumar* ...

co-author countries: India 🇮🇳
MeSH headings : Animals; Biosensing Techniques; Cell Line; Cell Physiological Phenomena / genetics; Fluorescence Resonance Energy Transfer; Fluorescent Dyes / metabolism; HEK293 Cells; Humans; Magnetic Resonance Spectroscopy; Mice; Phosphorylation / genetics; Proto-Oncogene Proteins c-fyn / chemistry; Proto-Oncogene Proteins c-fyn / genetics; Proto-Oncogene Proteins c-fyn / metabolism; Signal Transduction / genetics; Yeasts / genetics
Source: Web Of Science
Added: February 27, 2020

2020 journal article

Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display

BIOCONJUGATE CHEMISTRY, 31(10), 2325–2338.

By: J. Bowen n, A. Schloop n, G. Reeves*, S. Menegatti n & B. Rao n

MeSH headings : Animals; Cell Line; Cell-Penetrating Peptides / chemistry; Cell-Penetrating Peptides / genetics; Cell-Penetrating Peptides / pharmacokinetics; Drosophila melanogaster / embryology; Gene Library; Humans; Mice; Models, Molecular; NIH 3T3 Cells; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / pharmacokinetics; Permeability; RNA, Messenger / genetics
Sources: Web Of Science, ORCID
Added: December 21, 2020

2020 journal article

Genetically encoded live-cell sensor for tyrosinated microtubules

JOURNAL OF CELL BIOLOGY, 219(10).

co-author countries: France 🇫🇷 India 🇮🇳
MeSH headings : Colchicine / pharmacology; Cytoskeleton / drug effects; Cytoskeleton / genetics; HEK293 Cells; Humans; Microtubule-Associated Proteins / genetics; Microtubules / genetics; Nocodazole / pharmacology; Protein Processing, Post-Translational / drug effects; Protein Processing, Post-Translational / genetics; Tubulin / genetics; Tyrosine / drug effects; Tyrosine / genetics; Vincristine / pharmacology
Source: Web Of Science
Added: November 9, 2020

2020 journal article

Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display

ACS COMBINATORIAL SCIENCE, 22(10), 519–532.

By: K. Bacon n, A. Blain n, M. Burroughs n, N. McArthrur n, B. Rao n & S. Menegatti n

author keywords: cyclic peptides; interleukin-17 (IL-17); library screening; yeast-display libraries; affinity ligands
MeSH headings : Biophysical Phenomena; Cell Membrane / chemistry; Cyclization; Flow Cytometry; Humans; Interleukin-17 / chemistry; Molecular Docking Simulation; Peptide Library; Peptides, Cyclic / isolation & purification; Protein Binding; Protein Conformation; Protein Engineering; Recombinant Proteins / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / ultrastructure; Structure-Activity Relationship; Surface Properties
Sources: Web Of Science, ORCID
Added: November 9, 2020

2020 review

Past, Present, and Future of Affinity-based Cell Separation Technologies

[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.

By: K. Bacon n, A. Lavoie n, B. Rao n, M. Daniele n & S. Menegatti n

author keywords: Cell purification; Immunoaffinity; MACS; FACS; Microfluidics
MeSH headings : Cell Separation; Chromatography, Affinity; Flow Cytometry; Ligands; Regenerative Medicine
Sources: Web Of Science, ORCID
Added: August 3, 2020

2020 article

Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping

GENOTYPE PHENOTYPE COUPLING: METHODS AND PROTOCOLS, Vol. 2070, pp. 321–334.

By: C. Cruz-Teran n, K. Bacon n & B. Rao n

author keywords: Yeast surface display; Self-cleaving peptides; 2A peptides; Secretion; Ribosomal skipping; Sso7d
MeSH headings : Peptide Library; Peptides / metabolism; Protein Biosynthesis; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Ribosomes / metabolism; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
Source: Web Of Science
Added: September 7, 2020

2020 journal article

Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands

ACS COMBINATORIAL SCIENCE, 22(12), 738–744.

By: K. Bacon n, J. Bowen n, H. Reese n, B. Rao n & S. Menegatti n

author keywords: yeast surface display; yeast magnetization; peptide ligands; mRNA-display library; ligand discovery
MeSH headings : Ligands; Mitochondrial Membrane Transport Proteins / chemistry; Models, Molecular; Peptide Library; RNA, Messenger / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae Proteins / chemistry; Transcription Factors / chemistry
Sources: Web Of Science, ORCID
Added: January 11, 2021

2019 article

DERIVATION OF HUMAN TROPHOBLAST STEM CELLS FROM HUMAN PLURIPOTENT STEM CELLS

Mischler, A., Karakis, V., San Miguel, A., & Rao, B. (2019, August). PLACENTA, Vol. 83, pp. E59–E59.

By: A. Mischler n, V. Karakis n, A. San Miguel n & B. Rao n

Sources: Web Of Science, ORCID
Added: April 20, 2020

2019 journal article

Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation

SCIENCE SIGNALING, 12(584).

By: K. Tiruthani n, A. Mischler n, S. Ahmed*, J. Mahinthakumar n, J. Haugh n & B. Rao n

co-author countries: Bangladesh 🇧🇩
MeSH headings : Animals; Biosensing Techniques; Cell Line; Endocytosis; ErbB Receptors / genetics; ErbB Receptors / metabolism; Mice; Microscopy, Fluorescence; Phospholipase C gamma / genetics; Phospholipase C gamma / metabolism; Phosphorylation / genetics; src Homology Domains
Sources: Web Of Science, ORCID
Added: June 24, 2019

2019 journal article

Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders

ACS COMBINATORIAL SCIENCE, 21(12), 817–832.

By: K. Bacon n, M. Burroughs n, A. Blain n, S. Menegatti n & B. Rao n

author keywords: yeast magnetization; combinatorial library screening; ligand discovery; yeast surface display; membrane protein; Sso7d; nanobody
MeSH headings : Combinatorial Chemistry Techniques; Drug Evaluation, Preclinical; Ferric Compounds / chemistry; Ferric Compounds / pharmacology; Magnetic Phenomena; Mitochondria / drug effects; Mitochondria / metabolism; Mitochondrial Membrane Transport Proteins / antagonists & inhibitors; Mitochondrial Membrane Transport Proteins / isolation & purification; Mitochondrial Membrane Transport Proteins / metabolism; Nanoparticles / chemistry; Protein Binding / drug effects; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / antagonists & inhibitors; Saccharomyces cerevisiae Proteins / isolation & purification; Saccharomyces cerevisiae Proteins / metabolism; Small Molecule Libraries / chemistry; Small Molecule Libraries / pharmacology
Sources: Web Of Science, ORCID
Added: December 30, 2019

2018 journal article

An Engineered Sso7d Variant Enables Efficient Magnetization of Yeast Cells

ACS COMBINATORIAL SCIENCE, 20(10), 579–584.

By: C. Cruz-Teran n, K. Bacon n, N. McArthur n & B. Rao n

author keywords: Sso7d; magnetization; cell capture; yeast surface display; magnetic separation
MeSH headings : Amino Acid Sequence; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Cell Membrane / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Magnetic Phenomena; Magnetite Nanoparticles / chemistry; Mutation; Peptide Library; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
Source: Web Of Science
Added: October 29, 2018

2018 journal article

Kinetic Study of Degrafting Poly(methyl methacrylate) Brushes from Flat Substrates by Tetrabutylammonium Fluoride

MACROMOLECULES, 51(24), 10237–10245.

By: R. Patil n, J. Miles n, Y. Ko n, P. Datta n, B. Rao n, D. Kiserow*, J. Genzer n

Sources: Web Of Science, ORCID
Added: January 14, 2019

2017 journal article

A defined and serum-free culture system for trophoblast differentiation of human embryonic stem cells

Placenta, 57, 237–238.

By: A. Mischler n & B. Rao n

Source: Crossref
Added: February 24, 2020

2017 journal article

Design and Fabrication of Wettability Gradients with Tunable Profiles through Degrafting Organosilane Layers from Silica Surfaces by Tetrabutylammonium Fluoride

LANGMUIR, 33(51), 14556–14564.

By: J. Miles n, S. Schlenker n, Y. Ko n, R. Patil n, B. Rao n & J. Genzer n

Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

IFPA meeting 2016 workshop report I: Genomic communication, bioinformatics, trophoblast biology and transport systems

Placenta, 60, S5–S9.

By: C. Albrecht*, J. Baker*, C. Blundell*, S. Chavez*, L. Carbone*, L. Chamley*, R. Hannibal*, N. Illsley* ...

co-author countries: Australia 🇦🇺 Canada 🇨🇦 Switzerland 🇨🇭 Germany 🇩🇪 New Zealand 🇳🇿
author keywords: Transport; Trophoblast; Bioinformatics; Exosomes; OMICS; DNA methylation
MeSH headings : Animals; Biological Transport; Biomedical Research / methods; Biomedical Research / trends; Computational Biology / methods; Computational Biology / trends; Congresses as Topic; DNA Methylation; Exome; Female; Genomics / methods; Genomics / trends; Humans; International Agencies; Maternal-Fetal Exchange; Placenta / cytology; Placenta / pathology; Placenta / physiology; Placenta / physiopathology; Pregnancy; Pregnancy Complications / pathology; Pregnancy Complications / physiopathology; Societies, Scientific; Trophoblasts / cytology; Trophoblasts / pathology; Trophoblasts / physiology
Sources: Crossref, NC State University Libraries
Added: August 6, 2018

2017 journal article

Identification and characterization of a novel Sso7d scaffold-based binder against Notch1

SCIENTIFIC REPORTS, 7.

By: T. Gocha*, B. Rao n & R. DasGupta*

co-author countries: Singapore 🇸🇬
MeSH headings : Animals; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Binding, Competitive / drug effects; Carrier Proteins / genetics; Carrier Proteins / metabolism; Carrier Proteins / pharmacology; Cell Line, Tumor; Cell Membrane / drug effects; Cell Membrane / metabolism; Colorectal Neoplasms / metabolism; Colorectal Neoplasms / pathology; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; HCT116 Cells; HEK293 Cells; Humans; MCF-7 Cells; Protein Binding; RNA Interference; Receptor, Notch1 / antagonists & inhibitors; Receptor, Notch1 / genetics; Receptor, Notch1 / metabolism; Spheroids, Cellular / drug effects; Spheroids, Cellular / metabolism
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae

ACS SYNTHETIC BIOLOGY, 6(11), 2096–2107.

By: C. Cruz-Teran n, K. Tiruthani n, A. Mischler n & B. Rao n

author keywords: yeast surface display; secretion; ribosomal skipping; 2A peptide; Sso7d; glucose oxidase
MeSH headings : Cell Adhesion Molecules / genetics; Cell Adhesion Molecules / metabolism; Gene Expression; Peptide Library; Peptides / genetics; Peptides / metabolism; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism
Source: Web Of Science
Added: August 6, 2018

2016 journal article

BMP signaling plays a biphasic role during trophoblast differentiation of human embryonic stem cells

Placenta, 45, 99–100.

By: P. Sarkar n, A. Mischler n & B. Rao n

Source: Crossref
Added: February 24, 2020

2016 journal article

Combinatorial Pairwise Assembly Efficiently Generates High Affinity Binders and Enables a "Mix-and-Read" Detection Scheme

ACS SYNTHETIC BIOLOGY, 5(12), 1348–1354.

By: K. Carlin n, C. Cruz-Teran n, J. Kumar*, C. Gomes n & B. Rao n

co-author countries: India 🇮🇳
author keywords: Sso7d; binding affinity; yeast surface display; flexible linker; split GFP
MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Mutagenesis; Peptide Library; Plasmids / genetics; Protein Conformation; Protein Engineering; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Yeasts / genetics; Yeasts / metabolism
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.

By: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n, B. Rao n

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins

LANGMUIR, 32(34), 8660–8667.

By: C. Cruz-Teran n, K. Carlin n, K. Efimenko n, J. Genzer n & B. Rao n

Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(14), 8834–8848.

By: P. Sarkar n, S. Randall n, T. Collier n, A. Nero n, T. Russell n, D. Muddiman n, B. Rao n

MeSH headings : Activins / metabolism; Base Sequence; Cell Differentiation; Cell Lineage; DNA Methylation; DNA Primers; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Ephrin-B2 / genetics; Humans; Nodal Protein / metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Signal Transduction; Trophoblasts / cytology; Trophoblasts / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Avidity-mediated virus separation using a hyperthermophilic affinity ligand

BIOTECHNOLOGY PROGRESS, 29(1), 237–246.

By: M. Hussain n, D. Lockney n, R. Wang n, N. Gera n & B. Rao n

author keywords: avidity; affinity ligand; hyperthermophilic; multivalent
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Ligands; Molecular Weight; Plant Extracts; Plant Leaves / virology; Sulfolobus solfataricus / chemistry; Sulfolobus solfataricus / metabolism; Tobacco / virology; Tombusviridae / isolation & purification
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Generation and Properties of Antibacterial Coatings Based on Electrostatic Attachment of Silver Nanoparticles to Protein-Coated Polypropylene Fibers

ACS APPLIED MATERIALS & INTERFACES, 5(11), 5298–5306.

By: K. Goli n, N. Gera n, X. Liu n, B. Rao n, O. Rojas n & J. Genzer n

co-author countries: Finland 🇫🇮
author keywords: antibacterial; polypropylene; protein adsorption; nanoparticle; functional coating
MeSH headings : Anti-Bacterial Agents / chemistry; Coated Materials, Biocompatible / chemistry; Escherichia coli / drug effects; Escherichia coli Infections / prevention & control; Hydrophobic and Hydrophilic Interactions; Light; Metal Nanoparticles / chemistry; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Polypropylenes / chemistry; Proteins / chemistry; Silver / chemistry; Static Electricity; Ultraviolet Rays
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast

ACS APPLIED MATERIALS & INTERFACES, 5(19), 9349–9354.

By: M. Canbolat n, N. Gera n, C. Tang n, B. Monian n, B. Rao n, B. Pourdeyhimi n, S. Khan n

author keywords: electrospinning; nanofiber; protein; biocatalyst immobilization; yeast surface display
MeSH headings : Biocatalysis; Cell Survival / drug effects; Green Fluorescent Proteins; Microscopy, Electron, Scanning; Nanofibers / chemistry; Polymers / chemistry; Polymers / pharmacology; Polyvinyl Alcohol / chemistry; Polyvinyl Alcohol / pharmacology; Protein Conformation / drug effects; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / growth & development; Water / chemistry
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 review

Protein selection using yeast surface display

[Review of ]. METHODS, 60(1), 15–26.

By: N. Gera n, M. Hussain n & B. Rao n

author keywords: Yeast surface display; Protein engineering; Library screening; Biophysical characterization; Alternative scaffolds
MeSH headings : Combinatorial Chemistry Techniques; Flow Cytometry; Protein Engineering / methods; Yeasts / genetics; Yeasts / metabolism
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Scaffold Diversification Enhances Effectiveness of a Super library of Hyperthermophilic Proteins

ACS SYNTHETIC BIOLOGY, 2(1), 6–13.

By: M. Hussain n, N. Gera n, A. Hill n & B. Rao n

author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; alternate scaffold; mRNA display
MeSH headings : Biotechnology / methods; Carrier Proteins / genetics; Carrier Proteins / metabolism; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Models, Molecular; Peptide Library; Protein Conformation; Yeasts / genetics; Yeasts / metabolism
Source: Web Of Science
Added: August 6, 2018

2013 review

Trophoblast differentiation of human embryonic stem cells

[Review of ]. BIOTECHNOLOGY JOURNAL, 8(4), 421-+.

By: K. Tiruthani n, P. Sarkar n & B. Rao n

author keywords: Bone morphogenetic protein; CDX2; ELF5; hESC; Trophoblast
MeSH headings : Animals; Bone Morphogenetic Proteins / pharmacology; Cell Culture Techniques; Cell Differentiation / drug effects; Cell Differentiation / physiology; Embryonic Stem Cells / cytology; Embryonic Stem Cells / drug effects; Humans; Trophoblasts / cytology; Trophoblasts / drug effects
Source: Web Of Science
Added: August 6, 2018

2013 journal article

mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands

Biotechnology and Bioengineering, 110(3), 857–870.

By: S. Menegatti n, M. Hussain n, A. Naik n, R. Carbonell n & B. Rao n

author keywords: cyclic peptide; mRNA display; Fc binding; affinity ligand; library
MeSH headings : Humans; Immunoglobulin Fc Fragments / metabolism; Peptide Library; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / metabolism; Protein Binding; Protein Engineering / methods; RNA, Messenger / genetics; Solid-Phase Synthesis Techniques / methods
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold

PLOS ONE, 7(11).

MeSH headings : Carrier Proteins / genetics; Carrier Proteins / metabolism; Epitopes / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Histidine / genetics; Histidine / metabolism; Humans; Hydrogen-Ion Concentration; Immunoglobulin Fc Fragments / genetics; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / genetics; Immunoglobulin G / metabolism; Mutagenesis; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells

MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839.

By: P. Sarkar n, S. Randall n, D. Muddiman n & B. Rao n

MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned / analysis; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Mice; Proteins / analysis; Secretory Pathway; Subcellular Fractions / chemistry
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

The subcellular proteome of undifferentiated human embryonic stem cells

PROTEOMICS, 12(3), 421–430.

By: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n & B. Rao n

author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.

By: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold

JOURNAL OF MOLECULAR BIOLOGY, 409(4), 601–616.

By: N. Gera n, M. Hussain n, R. Wright n & B. Rao n

author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; stability; alternate scaffold
MeSH headings : Amino Acid Sequence; Animals; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Chickens; DNA Mutational Analysis; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Fluorescein / chemistry; Fluorescein / metabolism; Immunoglobulins / chemistry; Immunoglobulins / metabolism; Mice; Molecular Sequence Data; Muramidase / chemistry; Muramidase / metabolism; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protein Denaturation; Streptavidin / chemistry; Streptavidin / metabolism; Sulfolobus solfataricus / chemistry; beta Catenin / chemistry; beta Catenin / metabolism
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Role of Signaling Pathways and Epigenetic Factors in Lineage Determination During Human Embryonic Stem Cell Differentiation

Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, 1, 1.

By: P. Sarkar & B. Rao

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics

ANALYTICAL CHEMISTRY, 82(20), 8696–8702.

By: T. Collier n, P. Sarkar n, W. Franck n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Quantitative top-down proteomics of SILAC labeled human embryonic stem cells

Journal of the American Society for Mass Spectrometry, 21(6), 879–889.

By: T. Collier n, P. Sarkar n, B. Rao n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Animals; Arginine / chemistry; Arginine / metabolism; Carbon Isotopes; Cell Line; Embryonic Stem Cells / chemistry; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Lysine / chemistry; Lysine / metabolism; Mass Spectrometry / methods; Mice; Proteome / analysis; Proteomics / methods
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2009 journal article

Molecular aspects of cardiac differentiation in embryonic stem cells

Critical Reviews in Biomedical Engineering, 37(4-5), 283–320.

By: P. Sarkar n & B. Rao n

MeSH headings : Animals; Cell Differentiation / physiology; Cells, Cultured; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Models, Biological; Myocytes, Cardiac / cytology; Myocytes, Cardiac / physiology; Signal Transduction / physiology; Transcription Factors / metabolism
Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal

NATURE CELL BIOLOGY, 10(7), 837–848.

By: X. Varelas*, R. Sakuma*, P. Samavarchi-Tehrani*, R. Peerani*, B. Rao*, J. Dembowy*, M. Yaffe*, P. Zandstra*, J. Wrana*

co-author countries: Canada 🇨🇦
MeSH headings : Active Transport, Cell Nucleus / physiology; Acyltransferases; Animals; Cell Line; Cell Nucleus / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / physiology; Genes, Reporter; Humans; Mediator Complex; RNA, Small Interfering / genetics; RNA, Small Interfering / metabolism; Signal Transduction / physiology; Smad2 Protein / genetics; Smad2 Protein / metabolism; Smad3 Protein / genetics; Smad3 Protein / metabolism; Smad4 Protein / genetics; Smad4 Protein / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic; Transforming Growth Factor beta / metabolism
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Niche-mediated control of human embryonic stem cell self-renewal and differentiation

EMBO JOURNAL, 26(22), 4744–4755.

By: R. Peerani*, B. Rao*, C. Bauwens*, T. Yin*, G. Wood*, A. Nagy*, E. Kumacheva*, P. Zandstra*

co-author countries: Canada 🇨🇦
author keywords: embryonic stem cell; micropatterning; niche; self-renewal; Smad signaling
MeSH headings : Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins / metabolism; Cell Differentiation; Cell Line; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoderm / metabolism; Fibroblast Growth Factors / antagonists & inhibitors; Growth Differentiation Factor 3; Humans; Smad1 Protein / metabolism; Transforming Growth Factor beta / antagonists & inhibitors; Transforming Growth Factor beta / metabolism
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Improved mutants from directed evolution are biased to orthologous substitutions

PROTEIN ENGINEERING DESIGN & SELECTION, 19(6), 245–253.

By: J. Cochran*, Y. Kim*, S. Lippow*, B. Rao* & K. Wittrup*

co-author countries: Korea (Republic of) 🇰🇷
author keywords: epidermal growth factor; epidermal growth factor receptor; in vitro evolution; protein engineering; yeast display
MeSH headings : Base Sequence; Biosensing Techniques; Directed Molecular Evolution; Epidermal Growth Factor / chemistry; Epidermal Growth Factor / genetics; Epidermal Growth Factor / metabolism; ErbB Receptors / genetics; ErbB Receptors / metabolism; Fibroblasts / metabolism; Gene Library; Humans; Interleukin-2 / genetics; Interleukin-2 / metabolism; Mutagenesis; Mutation; Phylogeny; Protein Binding; Subtilisins / genetics; Subtilisins / metabolism; Transfection / methods; Yeasts / genetics
Source: Web Of Science
Added: August 6, 2018

2005 review

Culture development for human embryonic stem cell propagation: molecular aspects and challenges

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 16(5), 568–576.

By: B. Rao* & P. Zandstra*

co-author countries: Canada 🇨🇦
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Engineering protein therapeutics within a systems-level computational context

Nature Biotechnology, 23(2), 191–194.

By: B. Rao, D. Lauffenburger & K. Wittrup

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

High-Affinity CD25-Binding IL-2 Mutants Potently Stimulate Persistent T Cell Growth†

Biochemistry, 44(31), 10696–10701.

By: B. Rao*, I. Driver*, D. Lauffenburger* & K. Wittrup*

MeSH headings : Amino Acid Substitution / genetics; Cell Line; Cell Membrane / genetics; Cell Membrane / immunology; Cell Membrane / metabolism; Cell Proliferation; Directed Molecular Evolution; Humans; Interleukin-15 / metabolism; Interleukin-15 / physiology; Interleukin-2 / genetics; Interleukin-2 / metabolism; Interleukin-2 / physiology; Interleukin-2 Receptor alpha Subunit; Lymphocyte Activation / genetics; Mitogens / genetics; Mitogens / metabolism; Mitogens / physiology; Protein Binding / genetics; Receptors, Interleukin / metabolism; Receptors, Interleukin-15; Receptors, Interleukin-2 / metabolism; T-Lymphocytes / cytology; T-Lymphocytes / immunology
Source: Crossref
Added: August 28, 2020

2005 journal article

Integrating cell-level kinetic modeling into the design of engineered protein therapeutics

NATURE BIOTECHNOLOGY, 23(2), 191–194.

By: B. Rao*, D. Lauffenburger* & K. Wittrup*

MeSH headings : Cell Physiological Phenomena; Computer Simulation; Drug Delivery Systems / methods; Drug Design; Drug Therapy, Computer-Assisted / methods; Gene Expression Regulation / drug effects; Gene Expression Regulation / physiology; Kinetics; Models, Biological; Protein Engineering / methods
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect

Molecular Pharmacology, 66(4), 864–869.

By: B. Rao, I. Driver, D. Lauffenburger & K. Wittrup

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity

PROTEIN ENGINEERING, 16(12), 1081–1087.

By: B. Rao*, A. Girvin, T. Ciardelli, D. Lauffenburger & K. Wittrup

author keywords: alpha-receptor subunit binding affinity; interleukin-2 mutants; T-cell stimulation
MeSH headings : Cell Division / genetics; Cell Division / physiology; Cloning, Molecular; Interleukin-2 / genetics; Interleukin-2 / metabolism; Interleukin-2 Receptor alpha Subunit; Mutation; Protein Binding / genetics; Protein Binding / physiology; Receptors, Interleukin / metabolism; Saccharomyces cerevisiae
Source: Web Of Science
Added: August 6, 2018

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