@misc{woodrow_sheats_cooper_bayless_2023, title={Asthma: The Use of Animal Models and Their Translational Utility}, volume={12}, ISSN={["2073-4409"]}, url={https://doi.org/10.3390/cells12071091}, DOI={10.3390/cells12071091}, abstractNote={Asthma is characterized by chronic lower airway inflammation that results in airway remodeling, which can lead to a permanent decrease in lung function. The pathophysiology driving the development of asthma is complex and heterogenous. Animal models have been and continue to be essential for the discovery of molecular pathways driving the pathophysiology of asthma and novel therapeutic approaches. Animal models of asthma may be induced or naturally occurring. Species used to study asthma include mouse, rat, guinea pig, cat, dog, sheep, horse, and nonhuman primate. Some of the aspects to consider when evaluating any of these asthma models are cost, labor, reagent availability, regulatory burden, relevance to natural disease in humans, type of lower airway inflammation, biological samples available for testing, and ultimately whether the model can answer the research question(s). This review aims to discuss the animal models most available for asthma investigation, with an emphasis on describing the inciting antigen/allergen, inflammatory response induced, and its translation to human asthma.}, number={7}, journal={CELLS}, author={Woodrow, Jane Seymour and Sheats, M. Katie and Cooper, Bethanie and Bayless, Rosemary}, year={2023}, month={Apr} }
 @article{bayless_cooper_sheats_2022, title={Investigation of plasma cell-free DNA as a potential biomarker in horses}, volume={2}, ISSN={["1943-4936"]}, url={https://doi.org/10.1177/10406387221078047}, DOI={10.1177/10406387221078047}, abstractNote={Plasma cell-free DNA (cfDNA) is a biomarker of ischemia, systemic inflammation, and mortality in humans with gastrointestinal disease. Cell-free DNA has not been investigated as a biomarker for equine colic, to our knowledge. We hypothesized that cfDNA could be measured accurately in neat equine plasma using a benchtop fluorometer and that plasma cfDNA would be elevated in emergency patients compared to healthy horses. Plasma was obtained from blood collected in Roche DNA stabilizing tubes. We used the Qubit 4 fluorometer and 1× dsDNA HS assay kit to measure cfDNA concentration in neat patient plasma and following DNA extraction of plasma with a commercial kit. Assay precision and linearity of dilution were satisfactory for neat plasma cfDNA, but DNA spike and recovery results were variable. Further, cfDNA concentrations in paired neat plasma and extracted-plasma samples (n = 66) were not correlated. Median extracted-plasma cfDNA was higher in emergency patients (n = 50) and a subgroup of colic patients (n = 36), compared to healthy horses (n = 19). Our results with extracted-plasma samples provide proof of concept for further investigation of plasma cfDNA as a biomarker in horses.}, number={3}, journal={JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION}, publisher={SAGE Publications}, author={Bayless, Rosemary L. and Cooper, Bethanie L. and Sheats, M. Katie}, year={2022}, month={Feb} }