Works (62)

Updated: April 11th, 2023 10:13

2022 journal article

A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13

CELL HOST & MICROBE, 30(8), 1151-+.

By: E. Vialetto*, Y. Yu, S. Collins, K. Wandera, L. Barquist & C. Beisel

Source: Web Of Science
Added: November 21, 2022

2021 review

CRISPR technologies and the search for the PAM-free nuclease

[Review of ]. NATURE COMMUNICATIONS, 12(1).

By: D. Collias & C. Beisel

Source: Web Of Science
Added: February 22, 2021

2021 article

Coupling smartphone and CRISPR-Cas12a for digital and multiplexed nucleic acid detection

Yu, T., Zhang, S., Matei, R., Marx, W., Beisel, C. L., & Wei, Q. (2021, July 23). AICHE JOURNAL, Vol. 7.

By: T. Yu, S. Zhang, R. Matei*, W. Marx n, C. Beisel & Q. Wei

author keywords: Cas12a; CRISPR diagnostics; digital assay; point-of-care diagnostics; smartphone device
Sources: Web Of Science, ORCID
Added: August 2, 2021

2021 journal article

In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii

ACS SYNTHETIC BIOLOGY, 10(5), 1039–1052.

author keywords: Saccharomyces boulardii; probiotic engineering; metabolic engineering; colonization profile; in situ production; beta-carotene
Sources: Web Of Science, ORCID
Added: May 6, 2021

2021 journal article

Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch

NUCLEIC ACIDS RESEARCH, 49(5), 2985–2999.

By: S. Collins, W. Rostain*, C. Liao* & C. Beisel

Source: Web Of Science
Added: June 10, 2021

2020 journal article

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

SCIENCE ADVANCES, 6(29).

By: D. Collias, R. Leenay, R. Slotkowski, Z. Zuo*, S. Collins, B. McGirr n, J. Liu*, C. Beisel

Source: Web Of Science
Added: August 10, 2020

2020 journal article

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

METHODS, 172, 42–50.

By: K. Wandera*, S. Collins, F. Wimmer*, R. Marshall*, V. Noireaux* & C. Beisel

author keywords: Anti-CRISPR proteins; Cas9; Genome editing; sgRNA; TXTL
Source: Web Of Science
Added: April 6, 2020

2020 journal article

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

NUCLEIC ACIDS RESEARCH, 48(10), 5624–5638.

By: T. Jacobsen, F. Ttofali, C. Liao*, S. Manchalu*, B. Gray* & C. Beisel

Source: Web Of Science
Added: October 5, 2020

2020 journal article

Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(7).

By: S. Siedler*, M. Rau*, S. Bidstrup*, J. Vento, S. Aunsbjerg*, E. Bosma*, L. McNair*, C. Beisel, A. Neves*

author keywords: lactic acid bacteria; bioprotection; food spoilage; manganese starvation; genome editing; Lactobacillus
Source: Web Of Science
Added: April 20, 2020

2020 article

Methods for characterizing, applying, and teaching CRISPR-Cas systems

Beisel, C. L. (2020, February 1). METHODS, Vol. 172, pp. 1–2.

By: C. Beisel

Source: Web Of Science
Added: April 6, 2020

2020 journal article

Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems

PLOS ONE, 15(4).

By: T. Jacobsen, G. Yi n, H. Al Asafen n, A. Jermusyk n, C. Beisel & G. Reeves

Source: Web Of Science
Added: June 22, 2020

2020 article

Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Collins, S. P., & Beisel, C. L. (2020, September 3). MOLECULAR CELL, Vol. 79, pp. 703–704.

By: S. Collins & C. Beisel

Source: Web Of Science
Added: October 12, 2020

2019 journal article

An educational module to explore CRISPR technologies with a cell-free transcription-translation system

SYNTHETIC BIOLOGY, 4(1).

By: D. Collias, R. Marshall*, S. Collins, C. Beisel & V. Noireaux*

author keywords: CRISPR; Cas9; education modules; synthetic biology; TXTL
Source: Web Of Science
Added: February 27, 2020

2019 review

Barriers to genome editing with CRISPR in bacteria

[Review of ]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 46(9-10), 1327–1341.

By: J. Vento, N. Crook & C. Beisel

author keywords: Bacteria; Nuclease; Genome editing; CRISPR; Recombineering
Sources: Web Of Science, ORCID
Added: October 28, 2019

2019 article

CRATES: A one-step assembly method for Class 2 CRISPR arrays

TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - MOLECULAR METHODS, Vol. 629, pp. 493–511.

By: C. Liao*, R. Slotkowski & C. Beisel

Source: Web Of Science
Added: June 29, 2020

2019 journal article

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator

BIOTECHNOLOGY AND BIOENGINEERING, 116(5), 1139–1151.

By: A. Westbrook*, X. Tang*, R. Marshall*, C. Maxwell, J. Chappell*, D. Agrawal*, M. Dunlop*, V. Noireaux* ...

author keywords: Bayesian methods; CRISPRi; model-guided design; RNA-based circuits; sRNA
Source: Web Of Science
Added: April 15, 2019

2019 journal article

Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods

BIOTECHNOLOGY JOURNAL, 14(3).

author keywords: genome editing; lactobacilli; recombineering; shuttle plasmids
Source: Web Of Science
Added: March 18, 2019

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

NATURE COMMUNICATIONS, 10.

By: C. Liao n, F. Ttofali, R. Slotkowski, S. Denny, T. Cecil n, R. Leenay, A. Keung, C. Beisel

Source: Web Of Science
Added: July 22, 2019

2019 journal article

Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells

NATURE BIOTECHNOLOGY, 37(12), 1493-+.

By: A. Pickar-Oliver*, J. Black*, M. Lewis*, K. Mutchnick*, T. Klann*, K. Gilcrest*, M. Sitton*, C. Nelson* ...

Sources: Web Of Science, ORCID
Added: December 30, 2019

2019 journal article

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

FEMS MICROBIOLOGY LETTERS, 366(8).

By: T. Jacobsen, C. Liao* & C. Beisel

author keywords: Cas nuclease; Cpf1; CRISPR-Cas systems; PAM; TIDE; TXTL
Source: Web Of Science
Added: September 9, 2019

2019 journal article

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

RNA BIOLOGY, 16(4), 404–412.

By: C. Liao n, R. Slotkowski, T. Achmedov* & C. Beisel

author keywords: Cpf1; CRISPR; RNA structure; terminator; TXTL
Source: Web Of Science
Added: May 20, 2019

2018 journal article

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

Methods, 143, 48–57.

By: C. Maxwell, T. Jacobsen, R. Marshall, V. Noireaux & C. Beisel

author keywords: Cas9; Cas12a; PAM; sgRNA; crRNA; TXTL
Source: Crossref
Added: February 24, 2020

2018 journal article

Advances in CRISPR Technologies for Microbial Strain Engineering

Biotechnology Journal, 13(9), 1800460.

By: H. Alper & C. Beisel

Source: Crossref
Added: February 24, 2020

2018 journal article

Bacterial Adaptation to the Host's Diet Is a Key Evolutionary Force Shaping Drosophila-Lactobacillus LESymbiosis

CELL HOST & MICROBE, 24(1), 109-+.

By: M. Martino*, P. Joncour*, R. Leenay n, H. Gervais*, M. Shah, S. Hughes*, B. Gillet*, C. Beisel, F. Leulier*

Source: Web Of Science
Added: August 6, 2018

2018 journal article

CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9

MOLECULAR CELL, 69(5), 893-+.

By: G. Dugar*, R. Leenay n, S. Eisenbart*, T. Bischler*, B. Aul*, C. Beisel, C. Sharma*

Source: Web Of Science
Added: August 6, 2018

2018 journal article

CRISPR tool puts RNA on the record

Nature, 562(7727), 347–349.

By: C. Beisel

Source: Crossref
Added: November 21, 2020

2018 journal article

Mathematical Modeling of RNA-Based Architectures for Closed Loop Control of Gene Expression

ACS SYNTHETIC BIOLOGY, 7(5), 1219–1228.

By: D. Agrawal*, X. Tang*, A. Westbrook*, R. Marshall*, C. Maxwell n, J. Lucks*, V. Noireaux*, C. Beisel, M. Dunlop*, E. Franco*

author keywords: RNA; feedback; gene expression; mathematical modeling; control; sensitivity analysis
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

MOLECULAR CELL, 69(1), 146-+.

By: R. Marshall*, C. Maxwell n, S. Collins, T. Jacobsen, M. Luo n, M. Begemann*, B. Gray*, E. January* ...

Source: Web Of Science
Added: August 6, 2018

2018 article

Synthetic Biology Approaches to Engineer Probiotics and Members of the Human Microbiota for Biomedical Applications

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 20, Vol. 20, pp. 277–300.

By: J. Bober*, C. Beisel & N. Nair*

author keywords: probiotics; genetic tools; CRISPR; genome engineering; microbiome
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Advancing the design and delivery of CRISPR antimicrobials

Current Opinion in Biomedical Engineering, 4, 57–64.

By: J. Fagen n, D. Collias n, A. Singh n & C. Beisel

author keywords: Bacteriophage; Cas9; CRISPR-Cas systems; sgRNA; Tail-fiber proteins
Source: Crossref
Added: February 24, 2020

2017 review

Deciphering, Communicating, and Engineering the CRISPR PAM

[Review of ]. JOURNAL OF MOLECULAR BIOLOGY, 429(2), 177–191.

By: R. Leenay & C. Beisel

author keywords: Cas9; Cpf1; CRISPR-Cas systems; PFS; rPAM
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems

BIOTECHNOLOGY AND BIOENGINEERING, 114(9), 2137–2141.

By: R. Marshall*, C. Maxwell, S. Collins, C. Beisel & V. Noireaux*

author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Toward a genetic tool development pipeline for host-associated bacteria

Current Opinion in Microbiology, 38, 156–164.

By: M. Waller n, J. Bober*, N. Nair* & C. Beisel

Source: Crossref
Added: February 24, 2020

2017 journal article

What is the role of circuit design in the advancement of synthetic biology? Part 1 enabling real-world applications and therapeutics

Cell Systems, 4(4), 370–372.

By: Y. Chen, M. Shapiro, W. Ruder, H. Ye, S. Kiani, T. Moon, S. Raman, C. Beisel, C. Barnes

Source: NC State University Libraries
Added: August 6, 2018

2016 review

Current and future prospects for CRISPR-based tools in bacteria

[Review of ]. BIOTECHNOLOGY AND BIOENGINEERING, 113(5), 930–943.

By: M. Luo, R. Leenay & C. Beisel

author keywords: antimicrobials; Cas9; genetic control; genetic circuits; genome engineering; undomesticated microbes
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Engineering genes with CRISPR-Cas9

Chemical Engineering Progress , 112(9), 36–41.

By: M. Luo & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

By: R. Leenay, K. Maksimchuk n, R. Slotkowski, R. Agrawal, A. Gomaa n, A. Briner n, R. Barrangou, C. Beisel

Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 article

Rethinking the Hierarchy of Sugar Utilization in Bacteria

Beisel, C. L., & Afroz, T. (2016, February). JOURNAL OF BACTERIOLOGY, Vol. 198, pp. 374–376.

By: C. Beisel & T. Afroz

Source: Web Of Science
Added: August 6, 2018

2016 journal article

The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers

NUCLEIC ACIDS RESEARCH, 44(15), 7385–7394.

Source: Web Of Science
Added: August 6, 2018

2015 journal article

Cover Picture: Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing (Angew. Chem. Int. Ed. 41/2015)

Angewandte Chemie International Edition, 54(41), 11877–11877.

By: W. Sun, W. Ji, J. Hall, Q. Hu, C. Wang, C. Beisel, Z. Gu

Source: Crossref
Added: February 24, 2020

2015 journal article

Impact of Residual Inducer on Titratable Expression Systems

PLOS ONE, 10(9).

By: T. Afroz, M. Luo & C. Beisel

Source: Web Of Science
Added: August 6, 2018

2015 journal article

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

NUCLEIC ACIDS RESEARCH, 43(1), 674–681.

By: M. Luo, A. Mullis, R. Leenay & C. Beisel

Source: Web Of Science
Added: August 6, 2018

2015 journal article

Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing

Angewandte Chemie International Edition, 54(41), 12029–12033.

By: W. Sun, W. Ji n, J. Hall n, Q. Hu, C. Wang n, C. Beisel, Z. Gu

author keywords: CRISPR-Cas9; DNA; drug delivery; genome editing; nanoparticles
Sources: Crossref, Web Of Science
Added: August 6, 2018

2015 journal article

Trade-offs in Engineering Sugar Utilization Pathways for Titratable Control

ACS SYNTHETIC BIOLOGY, 4(2), 141–149.

By: T. Afroz, K. Biliouris*, K. Boykin, Y. Kaznessis* & C. Beisel

author keywords: carbon catabolism; Escherichia coli; feedback; inducible systems; linearization; systems biology
Source: Web Of Science
Added: August 6, 2018

2014 article

A CRISPR design for next-generation antimicrobials

GENOME BIOLOGY, Vol. 15.

By: C. Beisel, A. Gomaa n & R. Barrangou

Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Bacterial sugar utilization gives rise to distinct single-cell behaviours

MOLECULAR MICROBIOLOGY, 93(6), 1093–1103.

By: T. Afroz, K. Biliouris*, Y. Kaznessis* & C. Beisel

Source: Web Of Science
Added: August 6, 2018

2014 journal article

Construction of ligand-responsive microRNAs that operate through inhibition of Drosha processing

Artificial Riboswitches: Methods and Protocols, 1111, 259–267.

By: C. Beisel, R. Bloom & C. Smolke

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

MOLECULAR CELL, 56(2), 333–339.

By: A. Briner n, P. Donohoue*, A. Gomaa n, K. Selle, E. Slorach*, C. Nye*, R. Haurwitz*, C. Beisel, A. May*, R. Barrangou

Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

MBIO, 5(1).

By: A. Gomaa n, H. Klumpe n, M. Luo, K. Selle, R. Barrangou & C. Beisel

Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Understanding and exploiting feedback in synthetic biology

CHEMICAL ENGINEERING SCIENCE, 103, 79–90.

By: T. Afroz & C. Beisel

author keywords: Biological engineering; Feedback; Genetic circuit; Mathematical modeling; Process control; Synthetic biology
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Multiple factors dictate target selection by Hfq-binding small RNAs

The EMBO Journal, 31(8), 1961–1974.

By: C. Beisel, T. Updegrove*, B. Janson* & G. Storz*

author keywords: base-pairing; Escherichia coli; Spot 42; target prediction
Source: Crossref
Added: August 28, 2020

2011 journal article

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing

Nucleic Acids Research, 39(7), 2981–2994.

By: C. Beisel CL, C. Yy, . Hoff KG & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2011 article

Discriminating tastes: physiological contributions of the Hfq-binding small RNA Spot 42 to catabolite repression

RNA Biology, Vol. 8, pp. 1–5.

By: C. Beisel & G. Storz

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli

Molecular Cell, 41(3), 286–297.

By: C. Beisel & G. Storz*

Source: Crossref
Added: August 28, 2020

2011 journal article

The base pairing RNA Spot 42 participates in a multi-output feedforward loop to help enact catabolite repression in Escherichia coli

Molecular Cell, 41, 1–12.

By: B. Cl & S. G

Source: NC State University Libraries
Added: August 6, 2018

2010 review

Base pairing small RNAs and their roles in global regulatory networks

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 34(5), 866–882.

By: C. Beisel & G. Storz*

author keywords: sRNAs; regulatory circuits; gene regulation; Hfq; network motifs
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure [Including Theochem], 3(1).

By: H. Bayer TS, L. Beisel CL & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Design Principles for Riboswitch Function

PLOS COMPUTATIONAL BIOLOGY, 5(4).

By: C. Beisel & C. Smolke*

Source: Web Of Science
Added: August 6, 2018

2008 journal article

Model-guided design of ligand-regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4, 224.

By: B. Beisel CL, . Hoff KG & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Model‐guided design of ligand‐regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4(1), 224.

By: C. Beisel, T. Bayer*, K. Hoff* & C. Smolke*

author keywords: aptamer; mathematical modelling; RNA; shRNA; synthetic biology
Source: Crossref
Added: August 28, 2020

2005 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure, 730, 51–58.

By: D. Beisel CL & R. Pj

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure: THEOCHEM, 730(1-3), 51–58.

By: C. Beisel, M. Dowd* & P. Reilly*

Source: Crossref
Added: August 28, 2020