Works (63)

Updated: August 26th, 2023 21:16

2023 journal article

Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria

NATURE COMMUNICATIONS, 14(1).

By: D. Collias n, E. Vialetto*, J. Yu*, K. Co*, E. Almasi*, A. Ruettiger, T. Achmedov*, T. Strowig*, C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
Source: Web Of Science
Added: July 3, 2023

2022 journal article

A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13

CELL HOST & MICROBE, 30(8), 1151-+.

By: E. Vialetto*, Y. Yu*, S. Collins n, K. Wandera*, L. Barquist* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Autoimmunity / genetics; Bacteriophages / genetics; CRISPR-Cas Systems; Plasmids; RNA
Source: Web Of Science
Added: November 21, 2022

2021 review

CRISPR technologies and the search for the PAM-free nuclease

[Review of ]. NATURE COMMUNICATIONS, 12(1).

By: D. Collias n & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Binding Sites / genetics; CRISPR-Associated Protein 9 / classification; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems; Gene Editing / methods; Models, Genetic; Nucleotide Motifs; Phylogeny; Protein Engineering / methods; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism
Source: Web Of Science
Added: February 22, 2021

2021 article

Coupling smartphone and CRISPR-Cas12a for digital and multiplexed nucleic acid detection

Yu, T., Zhang, S., Matei, R., Marx, W., Beisel, C. L., & Wei, Q. (2021, July 23). AICHE JOURNAL, Vol. 7.

By: T. Yu n, S. Zhang n, R. Matei*, W. Marx n, C. Beisel n & Q. Wei n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Cas12a; CRISPR diagnostics; digital assay; point-of-care diagnostics; smartphone device
Sources: Web Of Science, ORCID
Added: August 2, 2021

2021 journal article

In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii

ACS SYNTHETIC BIOLOGY, 10(5), 1039–1052.

By: D. Durmusoglu n, I. Al'Abri n, S. Collins n, J. Cheng n, A. Eroglu n, C. Beisel n, N. Crook n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Saccharomyces boulardii; probiotic engineering; metabolic engineering; colonization profile; in situ production; beta-carotene
MeSH headings : Animals; Antineoplastic Agents / metabolism; CRISPR-Cas Systems; Feces / chemistry; Female; Gastrointestinal Microbiome; Gastrointestinal Tract / metabolism; Gastrointestinal Tract / microbiology; Gene Editing / methods; Gene Expression; Indoles / metabolism; Male; Metabolic Engineering / methods; Mice; Mice, Inbred C57BL; Microorganisms, Genetically-Modified; Multigene Family; Plasmids / genetics; Probiotics / metabolism; Promoter Regions, Genetic; Provitamins / biosynthesis; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces cerevisiae / genetics; beta Carotene / biosynthesis
Sources: Web Of Science, ORCID
Added: May 6, 2021

2021 journal article

Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch

NUCLEIC ACIDS RESEARCH, 49(5), 2985–2999.

By: S. Collins n, W. Rostain*, C. Liao* & C. Beisel n

co-author countries: Germany 🇩🇪 France 🇫🇷 United States of America 🇺🇸
MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems; DNA / chemistry; Endodeoxyribonucleases / metabolism; Escherichia coli / genetics; Phosphotransferases (Alcohol Group Acceptor) / genetics; Protein Biosynthesis; RNA / chemistry; RNA / metabolism; RNA, Messenger / metabolism; Transcription, Genetic
Source: Web Of Science
Added: June 10, 2021

2020 journal article

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

SCIENCE ADVANCES, 6(29).

By: D. Collias n, R. Leenay n, R. Slotkowski n, Z. Zuo*, S. Collins n, B. McGirr n, J. Liu*, C. Beisel n

co-author countries: China 🇨🇳 Germany 🇩🇪 United States of America 🇺🇸
Source: Web Of Science
Added: August 10, 2020

2020 journal article

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

METHODS, 172, 42–50.

By: K. Wandera*, S. Collins n, F. Wimmer*, R. Marshall*, V. Noireaux* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Anti-CRISPR proteins; Cas9; Genome editing; sgRNA; TXTL
MeSH headings : Bacteriophages / metabolism; CRISPR-Associated Protein 9 / antagonists & inhibitors; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems / genetics; Enzyme Assays / instrumentation; Enzyme Assays / methods; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / immunology; Escherichia coli / virology; Escherichia coli Proteins / antagonists & inhibitors; Escherichia coli Proteins / metabolism; Fluorescence; Gene Editing / methods; Genes, Reporter / genetics; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Protein Biosynthesis; Transcription, Genetic; Viral Proteins / metabolism
Source: Web Of Science
Added: April 6, 2020

2020 journal article

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

NUCLEIC ACIDS RESEARCH, 48(10), 5624–5638.

By: T. Jacobsen n, F. Ttofali n, C. Liao*, S. Manchalu*, B. Gray* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / classification; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / classification; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism; DNA Cleavage; Endodeoxyribonucleases / chemistry; Endodeoxyribonucleases / classification; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; HEK293 Cells; Humans; Mutation; Phylogeny; Prevotella / enzymology; Protein Biosynthesis; Protein Domains; Transcription, Genetic
Source: Web Of Science
Added: October 5, 2020

2020 journal article

Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(7).

co-author countries: Germany 🇩🇪 Denmark 🇩🇰 United States of America 🇺🇸
author keywords: lactic acid bacteria; bioprotection; food spoilage; manganese starvation; genome editing; Lactobacillus
MeSH headings : Cultured Milk Products / microbiology; Food Microbiology; Fungi / physiology; Lactobacillus / physiology; Yeasts / physiology
Source: Web Of Science
Added: April 20, 2020

2020 article

Methods for characterizing, applying, and teaching CRISPR-Cas systems

Beisel, C. L. (2020, February 1). METHODS, Vol. 172, pp. 1–2.

By: C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Bacteria / genetics; CRISPR-Cas Systems / genetics; Gene Editing / methods; Molecular Biology / education; Molecular Biology / methods; Teaching
Source: Web Of Science
Added: April 6, 2020

2020 journal article

Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems

PLOS ONE, 15(4).

By: T. Jacobsen n, G. Yi n, H. Al Asafen n, A. Jermusyk n, C. Beisel n & G. Reeves n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Animals; Drosophila / genetics; Eukaryota / genetics; Eukaryota / metabolism; Eukaryotic Cells / metabolism; Gene Expression / genetics; Gene Expression / physiology; Gene Expression Regulation / genetics; Gene Expression Regulation / physiology; Genetic Engineering / methods; Nucleic Acid Conformation; Proof of Concept Study; RNA, Catalytic / genetics; RNA, Catalytic / physiology; RNA, Messenger / metabolism; Synthetic Biology / methods
Source: Web Of Science
Added: June 22, 2020

2020 article

Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Collins, S. P., & Beisel, C. L. (2020, September 3). MOLECULAR CELL, Vol. 79, pp. 703–704.

By: S. Collins n & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Cytosine; DNA, Single-Stranded; Deamination; Gene Editing
Source: Web Of Science
Added: October 12, 2020

2019 journal article

An educational module to explore CRISPR technologies with a cell-free transcription-translation system

SYNTHETIC BIOLOGY, 4(1).

By: D. Collias n, R. Marshall*, S. Collins n, C. Beisel* & V. Noireaux*

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: CRISPR; Cas9; education modules; synthetic biology; TXTL
Source: Web Of Science
Added: February 27, 2020

2019 review

Barriers to genome editing with CRISPR in bacteria

[Review of ]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 46(9-10), 1327–1341.

By: J. Vento n, N. Crook n & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Bacteria; Nuclease; Genome editing; CRISPR; Recombineering
MeSH headings : Bacteria / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing; Humans
Sources: Web Of Science, ORCID
Added: October 28, 2019

2019 article

CRATES: A one-step assembly method for Class 2 CRISPR arrays

TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - MOLECULAR METHODS, Vol. 629, pp. 493–511.

By: C. Liao*, R. Slotkowski n & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA Cleavage; Escherichia coli / genetics; Gene Editing / methods; Plasmids / genetics
Source: Web Of Science
Added: June 29, 2020

2019 journal article

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator

BIOTECHNOLOGY AND BIOENGINEERING, 116(5), 1139–1151.

By: A. Westbrook*, X. Tang*, R. Marshall*, C. Maxwell n, J. Chappell*, D. Agrawal*, M. Dunlop*, V. Noireaux* ...

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Bayesian methods; CRISPRi; model-guided design; RNA-based circuits; sRNA
MeSH headings : CRISPR-Cas Systems; Cell-Free System / chemistry; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats; Models, Chemical; RNA / chemistry; RNA / metabolism; Transcription, Genetic; Transcriptional Activation
Source: Web Of Science
Added: April 15, 2019

2019 journal article

Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods

BIOTECHNOLOGY JOURNAL, 14(3).

co-author countries: Germany 🇩🇪 France 🇫🇷
author keywords: genome editing; lactobacilli; recombineering; shuttle plasmids
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Gene Editing / methods; Lactobacillus plantarum / genetics; Plasmids / genetics; Recombinases / genetics; Recombination, Genetic / genetics
Source: Web Of Science
Added: March 18, 2019

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

NATURE COMMUNICATIONS, 10.

By: C. Liao n, F. Ttofali n, R. Slotkowski n, S. Denny n, T. Cecil n, R. Leenay n, A. Keung n, C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
Source: Web Of Science
Added: July 22, 2019

2019 journal article

Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells

NATURE BIOTECHNOLOGY, 37(12), 1493-+.

By: A. Pickar-Oliver*, J. Black*, M. Lewis*, K. Mutchnick*, T. Klann*, K. Gilcrest*, M. Sitton*, C. Nelson* ...

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : CRISPR-Cas Systems / genetics; Escherichia coli / genetics; Genetic Engineering / methods; HEK293 Cells; Humans; Listeria monocytogenes / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
Sources: Web Of Science, ORCID
Added: December 30, 2019

2019 journal article

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

FEMS MICROBIOLOGY LETTERS, 366(8).

By: T. Jacobsen n, C. Liao* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Cas nuclease; Cpf1; CRISPR-Cas systems; PAM; TIDE; TXTL
MeSH headings : Acidaminococcus / enzymology; Acidaminococcus / genetics; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems; Catalytic Domain; DNA Cleavage; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; Endonucleases / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Gene Editing; HEK293 Cells; Humans; Nucleotide Motifs; Plasmids / genetics
Source: Web Of Science
Added: September 9, 2019

2019 journal article

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

RNA BIOLOGY, 16(4), 404–412.

By: C. Liao n, R. Slotkowski n, T. Achmedov* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Cpf1; CRISPR; RNA structure; terminator; TXTL
MeSH headings : CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA Cleavage; DNA, Intergenic / genetics; Francisella / genetics; Nucleic Acid Conformation; RNA Processing, Post-Transcriptional / genetics; RNA, Guide, Kinetoplastida / metabolism; Rho Factor / metabolism; Terminal Repeat Sequences / genetics; Transcription Termination, Genetic
Source: Web Of Science
Added: May 20, 2019

2018 journal article

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

Methods, 143, 48–57.

By: C. Maxwell n, T. Jacobsen n, R. Marshall*, V. Noireaux* & C. Beisel n

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
author keywords: Cas9; Cas12a; PAM; sgRNA; crRNA; TXTL
MeSH headings : Amino Acid Motifs; Biotechnology / methods; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Computational Biology / methods; DNA / genetics; Escherichia coli; Gene Library; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / metabolism; High-Throughput Nucleotide Sequencing / instrumentation; High-Throughput Nucleotide Sequencing / methods; Neisseria meningitidis; Plasmids / genetics; Protein Biosynthesis / genetics; RNA, Guide, Kinetoplastida / genetics; Sequence Analysis, DNA / instrumentation; Sequence Analysis, DNA / methods; Transcription, Genetic / genetics
Source: Crossref
Added: February 24, 2020

2018 journal article

Advances in CRISPR Technologies for Microbial Strain Engineering

Biotechnology Journal, 13(9), 1800460.

By: H. Alper* & C. Beisel*

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Bacteria / genetics; CRISPR-Cas Systems; Fungi / genetics; Gene Editing / methods; Genetic Engineering / methods
Source: Crossref
Added: February 24, 2020

2018 journal article

Bacterial Adaptation to the Host's Diet Is a Key Evolutionary Force Shaping Drosophila-Lactobacillus LESymbiosis

CELL HOST & MICROBE, 24(1), 109-+.

By: M. Martino*, P. Joncour*, R. Leenay n, H. Gervais*, M. Shah n, S. Hughes*, B. Gillet*, C. Beisel n, F. Leulier*

co-author countries: Germany 🇩🇪 France 🇫🇷 United States of America 🇺🇸
MeSH headings : Acetate Kinase / genetics; Acetate Kinase / metabolism; Adaptation, Physiological; Animals; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Drosophila melanogaster / microbiology; Evolution, Molecular; Glutamine / analogs & derivatives; Glutamine / metabolism; Host Microbial Interactions; Lactobacillus plantarum / genetics; Lactobacillus plantarum / growth & development; Larva / microbiology; Microbiota; Mutation; Symbiosis
Source: Web Of Science
Added: August 6, 2018

2018 journal article

CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9

MOLECULAR CELL, 69(5), 893-+.

By: G. Dugar*, R. Leenay n, S. Eisenbart*, T. Bischler*, B. Aul*, C. Beisel n, C. Sharma*

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems / physiology; Campylobacter jejuni / enzymology; Campylobacter jejuni / genetics; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Protein Domains; RNA Stability / physiology; RNA, Bacterial / genetics; RNA, Bacterial / metabolism; RNA, Messenger / genetics; RNA, Messenger / metabolism
Source: Web Of Science
Added: August 6, 2018

2018 journal article

CRISPR tool puts RNA on the record

Nature, 562(7727), 347–349.

By: C. Beisel*

MeSH headings : CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing; RNA
Source: Crossref
Added: November 21, 2020

2018 journal article

Mathematical Modeling of RNA-Based Architectures for Closed Loop Control of Gene Expression

ACS SYNTHETIC BIOLOGY, 7(5), 1219–1228.

By: D. Agrawal*, X. Tang*, A. Westbrook*, R. Marshall*, C. Maxwell n, J. Lucks*, V. Noireaux*, C. Beisel n, M. Dunlop*, E. Franco*

co-author countries: United States of America 🇺🇸
author keywords: RNA; feedback; gene expression; mathematical modeling; control; sensitivity analysis
MeSH headings : CRISPR-Cas Systems; Computational Biology / methods; Feedback, Physiological / physiology; Gene Expression Regulation; Models, Theoretical; RNA / chemistry; Synthetic Biology / methods
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

MOLECULAR CELL, 69(1), 146-+.

By: R. Marshall*, C. Maxwell n, S. Collins n, T. Jacobsen n, M. Luo n, M. Begemann*, B. Gray*, E. January* ...

co-author countries: United States of America 🇺🇸
MeSH headings : CRISPR-Cas Systems / genetics; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Endonucleases / metabolism; Escherichia coli / genetics; Genetic Engineering / methods; Oryza / genetics; Protein Biosynthesis / genetics; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic / genetics
Source: Web Of Science
Added: August 6, 2018

2018 article

Synthetic Biology Approaches to Engineer Probiotics and Members of the Human Microbiota for Biomedical Applications

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 20, Vol. 20, pp. 277–300.

By: J. Bober*, C. Beisel n & N. Nair*

co-author countries: United States of America 🇺🇸
author keywords: probiotics; genetic tools; CRISPR; genome engineering; microbiome
MeSH headings : Animals; Bacteroides; CRISPR-Cas Systems; Gene Editing; Genetic Engineering; Genomics; Humans; Immune System; Lactic Acid / chemistry; Microbiota; Probiotics / chemistry; Probiotics / therapeutic use; Synthetic Biology / methods
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Advancing the design and delivery of CRISPR antimicrobials

Current Opinion in Biomedical Engineering, 4, 57–64.

By: J. Fagen n, D. Collias n, A. Singh n & C. Beisel n

co-author countries: United States of America 🇺🇸
author keywords: Bacteriophage; Cas9; CRISPR-Cas systems; sgRNA; Tail-fiber proteins
Source: Crossref
Added: February 24, 2020

2017 review

Deciphering, Communicating, and Engineering the CRISPR PAM

[Review of ]. JOURNAL OF MOLECULAR BIOLOGY, 429(2), 177–191.

By: R. Leenay n & C. Beisel n

co-author countries: United States of America 🇺🇸
author keywords: Cas9; Cpf1; CRISPR-Cas systems; PFS; rPAM
MeSH headings : Amino Acid Motifs; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Gene Library; Genetic Loci; Plasmids / genetics; Protein Engineering; RNA / genetics; RNA / metabolism; Staphylococcus epidermidis / genetics; Streptococcus thermophilus / genetics
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems

BIOTECHNOLOGY AND BIOENGINEERING, 114(9), 2137–2141.

By: R. Marshall*, C. Maxwell n, S. Collins n, C. Beisel n & V. Noireaux*

co-author countries: United States of America 🇺🇸
author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
MeSH headings : Cell-Free System / physiology; DNA, Bacterial / genetics; Escherichia coli / genetics; Exodeoxyribonuclease V / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Protein Biosynthesis / genetics; Transcription, Genetic / genetics
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Toward a genetic tool development pipeline for host-associated bacteria

Current Opinion in Microbiology, 38, 156–164.

By: M. Waller n, J. Bober*, N. Nair* & C. Beisel n

co-author countries: United States of America 🇺🇸
MeSH headings : Bacteria / genetics; Gastrointestinal Microbiome; Genetics, Microbial / methods; Host-Pathogen Interactions; Humans; Molecular Biology / methods; Symbiosis
Source: Crossref
Added: February 24, 2020

2017 journal article

What is the role of circuit design in the advancement of synthetic biology? Part 1 enabling real-world applications and therapeutics

Cell Systems, 4(4), 370–372.

By: Y. Chen, M. Shapiro, W. Ruder, H. Ye, S. Kiani, T. Moon, S. Raman, C. Beisel, C. Barnes

Source: NC State University Libraries
Added: August 6, 2018

2016 review

Current and future prospects for CRISPR-based tools in bacteria

[Review of ]. BIOTECHNOLOGY AND BIOENGINEERING, 113(5), 930–943.

By: M. Luo n, R. Leenay* & C. Beisel*

co-author countries: United States of America 🇺🇸
author keywords: antimicrobials; Cas9; genetic control; genetic circuits; genome engineering; undomesticated microbes
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / genetics; Bacterial Infections / drug therapy; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Drug Discovery; Drug Resistance, Bacterial; Gene Editing / methods; Gene Expression Regulation, Bacterial; Genetic Engineering / methods; Genome, Bacterial; Humans
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Engineering genes with CRISPR-Cas9

Chemical Engineering Progress , 112(9), 36–41.

By: M. Luo & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

By: R. Leenay n, K. Maksimchuk n, R. Slotkowski n, R. Agrawal n, A. Gomaa n, A. Briner n, R. Barrangou n, C. Beisel n

co-author countries: Egypt 🇪🇬 United States of America 🇺🇸
MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 article

Rethinking the Hierarchy of Sugar Utilization in Bacteria

Beisel, C. L., & Afroz, T. (2016, February). JOURNAL OF BACTERIOLOGY, Vol. 198, pp. 374–376.

By: C. Beisel n & T. Afroz n

co-author countries: United States of America 🇺🇸
MeSH headings : Bacteria / metabolism; Carbohydrate Metabolism / physiology; Carbohydrates / classification; Gene Expression Regulation, Bacterial
Source: Web Of Science
Added: August 6, 2018

2016 journal article

The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers

NUCLEIC ACIDS RESEARCH, 44(15), 7385–7394.

co-author countries: United States of America 🇺🇸
MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems / genetics; DNA / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial; Gene Silencing; Macromolecular Substances / chemistry; Macromolecular Substances / metabolism; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Cover Picture: Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing (Angew. Chem. Int. Ed. 41/2015)

Angewandte Chemie International Edition, 54(41), 11877–11877.

By: W. Sun n, W. Ji n, J. Hall n, Q. Hu n, C. Wang n, C. Beisel n, Z. Gu n

co-author countries: United States of America 🇺🇸
Source: Crossref
Added: February 24, 2020

2015 journal article

Impact of Residual Inducer on Titratable Expression Systems

PLOS ONE, 10(9).

By: T. Afroz n, M. Luo n & C. Beisel n

co-author countries: United States of America 🇺🇸
MeSH headings : Arabinose / biosynthesis; Arabinose / genetics; Bacterial Proteins / biosynthesis; Bacterial Proteins / genetics; Escherichia coli; Flow Cytometry; Gene Expression Regulation, Bacterial; Models, Theoretical; Plasmids; Promoter Regions, Genetic; Riboswitch / genetics; Thiamine / genetics; Thiamine Pyrophosphate / biosynthesis; Thiamine Pyrophosphate / genetics
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

NUCLEIC ACIDS RESEARCH, 43(1), 674–681.

By: M. Luo n, A. Mullis n, R. Leenay n & C. Beisel n

co-author countries: United States of America 🇺🇸
MeSH headings : CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Cell Engineering; Escherichia coli / genetics; Gene Deletion; Transcription, Genetic
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing

Angewandte Chemie International Edition, 54(41), 12029–12033.

By: W. Sun n, W. Ji n, J. Hall n, Q. Hu n, C. Wang n, C. Beisel n, Z. Gu n

co-author countries: United States of America 🇺🇸
author keywords: CRISPR-Cas9; DNA; drug delivery; genome editing; nanoparticles
MeSH headings : Animals; CRISPR-Associated Proteins / administration & dosage; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Cell Line; Clustered Regularly Interspaced Short Palindromic Repeats; DNA / administration & dosage; DNA / chemistry; DNA / genetics; Green Fluorescent Proteins / genetics; Humans; Mice, Nude; Nanoparticles / chemistry; Neoplasms / genetics; Neoplasms / therapy; RNA, Guide, Kinetoplastida / administration & dosage; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; Streptococcus pyogenes / chemistry; Streptococcus pyogenes / genetics
Sources: Crossref, Web Of Science
Added: August 6, 2018

2015 journal article

Trade-offs in Engineering Sugar Utilization Pathways for Titratable Control

ACS SYNTHETIC BIOLOGY, 4(2), 141–149.

By: T. Afroz n, K. Biliouris*, K. Boykin n, Y. Kaznessis* & C. Beisel n

co-author countries: United States of America 🇺🇸
author keywords: carbon catabolism; Escherichia coli; feedback; inducible systems; linearization; systems biology
MeSH headings : Arabinose / metabolism; Carbohydrate Metabolism / physiology; Escherichia coli / metabolism; Metabolic Engineering; Models, Theoretical
Source: Web Of Science
Added: August 6, 2018

2014 article

A CRISPR design for next-generation antimicrobials

GENOME BIOLOGY, Vol. 15.

By: C. Beisel n, A. Gomaa n & R. Barrangou n

co-author countries: Egypt 🇪🇬 United States of America 🇺🇸
MeSH headings : Animals; Anti-Infective Agents / administration & dosage; CRISPR-Cas Systems; Drug Resistance, Microbial / genetics; Enterohemorrhagic Escherichia coli / genetics; Gene Targeting; RNA, Guide, Kinetoplastida / genetics; Ribonucleases / genetics
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Bacterial sugar utilization gives rise to distinct single-cell behaviours

MOLECULAR MICROBIOLOGY, 93(6), 1093–1103.

By: T. Afroz n, K. Biliouris*, Y. Kaznessis* & C. Beisel n

co-author countries: United States of America 🇺🇸
MeSH headings : Carbohydrate Metabolism; Escherichia coli / cytology; Escherichia coli / metabolism; Gene Expression Regulation, Bacterial; Metabolic Networks and Pathways; Models, Biological; Single-Cell Analysis / methods
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Construction of ligand-responsive microRNAs that operate through inhibition of Drosha processing

Artificial Riboswitches: Methods and Protocols, 1111, 259–267.

By: C. Beisel, R. Bloom & C. Smolke

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

MOLECULAR CELL, 56(2), 333–339.

By: A. Briner n, P. Donohoue*, A. Gomaa n, K. Selle n, E. Slorach*, C. Nye*, R. Haurwitz*, C. Beisel n, A. May*, R. Barrangou n

co-author countries: Egypt 🇪🇬 United States of America 🇺🇸
MeSH headings : Bacterial Proteins / chemistry; CRISPR-Associated Protein 9; CRISPR-Associated Proteins / chemistry; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; DNA / chemistry; DNA Cleavage; Endonucleases / chemistry; Genetic Engineering / methods; HEK293 Cells; Humans; Nucleic Acid Conformation; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / ultrastructure
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

MBIO, 5(1).

By: A. Gomaa n, H. Klumpe n, M. Luo n, K. Selle n, R. Barrangou n & C. Beisel n

co-author countries: United States of America 🇺🇸
MeSH headings : CRISPR-Cas Systems; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Escherichia coli / genetics; Genome, Bacterial; Hydrolysis; Microbial Viability
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Understanding and exploiting feedback in synthetic biology

CHEMICAL ENGINEERING SCIENCE, 103, 79–90.

By: T. Afroz n & C. Beisel n

co-author countries: United States of America 🇺🇸
author keywords: Biological engineering; Feedback; Genetic circuit; Mathematical modeling; Process control; Synthetic biology
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Multiple factors dictate target selection by Hfq-binding small RNAs

The EMBO Journal, 31(8), 1961–1974.

By: C. Beisel*, T. Updegrove*, B. Janson* & G. Storz*

co-author countries: United States of America 🇺🇸
author keywords: base-pairing; Escherichia coli; Spot 42; target prediction
MeSH headings : Base Pairing; Binding Sites; Escherichia coli / physiology; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial; Host Factor 1 Protein / metabolism; MicroRNAs / metabolism; Nucleic Acid Conformation; Protein Binding; Sequence Analysis
Source: Crossref
Added: August 28, 2020

2011 journal article

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing

Nucleic Acids Research, 39(7), 2981–2994.

By: C. Beisel CL, C. Yy, . Hoff KG & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2011 article

Discriminating tastes: physiological contributions of the Hfq-binding small RNA Spot 42 to catabolite repression

RNA Biology, Vol. 8, pp. 1–5.

By: C. Beisel & G. Storz

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli

Molecular Cell, 41(3), 286–297.

By: C. Beisel* & G. Storz*

co-author countries: United States of America 🇺🇸
MeSH headings : Base Pairing; Base Sequence; Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / growth & development; Escherichia coli / metabolism; Gene Expression Regulation, Bacterial; Microbial Viability; Molecular Sequence Data; Operon; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Messenger / chemistry; RNA, Messenger / genetics
Source: Crossref
Added: August 28, 2020

2011 journal article

The base pairing RNA Spot 42 participates in a multi-output feedforward loop to help enact catabolite repression in Escherichia coli

Molecular Cell, 41, 1–12.

By: B. Cl & S. G

Source: NC State University Libraries
Added: August 6, 2018

2010 review

Base pairing small RNAs and their roles in global regulatory networks

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 34(5), 866–882.

By: C. Beisel* & G. Storz*

co-author countries: United States of America 🇺🇸
author keywords: sRNAs; regulatory circuits; gene regulation; Hfq; network motifs
MeSH headings : Bacteria / genetics; Base Pairing / genetics; Gene Expression Regulation, Bacterial; Gene Regulatory Networks; RNA, Bacterial / genetics; RNA, Bacterial / metabolism; RNA, Small Untranslated / genetics; RNA, Small Untranslated / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure [Including Theochem], 3(1).

By: H. Bayer TS, L. Beisel CL & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Design Principles for Riboswitch Function

PLOS COMPUTATIONAL BIOLOGY, 5(4).

By: C. Beisel* & C. Smolke*

co-author countries: United States of America 🇺🇸
MeSH headings : Base Sequence; Gene Expression Regulation / genetics; Models, Genetic; Molecular Sequence Data; RNA, Messenger / genetics; Regulatory Sequences, Ribonucleic Acid; Ribosomes / genetics
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Model-guided design of ligand-regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4, 224.

By: B. Beisel CL, . Hoff KG & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Model‐guided design of ligand‐regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4(1), 224.

By: C. Beisel*, T. Bayer*, K. Hoff* & C. Smolke*

co-author countries: United States of America 🇺🇸
author keywords: aptamer; mathematical modelling; RNA; shRNA; synthetic biology
MeSH headings : Aptamers, Nucleotide / chemistry; Biotechnology / methods; Drug Design; Ligands; RNA Interference / drug effects; RNA, Small Interfering / chemical synthesis; RNA, Small Interfering / pharmacology
Source: Crossref
Added: August 28, 2020

2005 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure: THEOCHEM, 730(1-3), 51–58.

By: C. Beisel*, M. Dowd* & P. Reilly*

co-author countries: United States of America 🇺🇸
Source: Crossref
Added: August 28, 2020

2005 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure, 730, 51–58.

By: D. Beisel CL & R. Pj

Source: NC State University Libraries
Added: August 6, 2018