Chase Beisel

A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13

CELL HOST & MICROBE, 30(8), 1151-+.

CRISPR technologies and the search for the PAM-free nuclease

[Review of ]. NATURE COMMUNICATIONS, 12(1).

Coupling smartphone and CRISPR-Cas12a for digital and multiplexed nucleic acid detection

Yu, T., Zhang, S., Matei, R., Marx, W., Beisel, C. L., & Wei, Q. (2021, July 23). AICHE JOURNAL, Vol. 7.

In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii

ACS SYNTHETIC BIOLOGY, 10(5), 1039–1052.

Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch

NUCLEIC ACIDS RESEARCH, 49(5), 2985–2999.

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

SCIENCE ADVANCES, 6(29).

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

METHODS, 172, 42–50.

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

NUCLEIC ACIDS RESEARCH, 48(10), 5624–5638.

Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(7).

Methods for characterizing, applying, and teaching CRISPR-Cas systems

Beisel, C. L. (2020, February 1). METHODS, Vol. 172, pp. 1–2.

Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems

PLOS ONE, 15(4).

Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Collins, S. P., & Beisel, C. L. (2020, September 3). MOLECULAR CELL, Vol. 79, pp. 703–704.

An educational module to explore CRISPR technologies with a cell-free transcription-translation system

SYNTHETIC BIOLOGY, 4(1).

Barriers to genome editing with CRISPR in bacteria

[Review of ]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 46(9-10), 1327–1341.

CRATES: A one-step assembly method for Class 2 CRISPR arrays

TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - MOLECULAR METHODS, Vol. 629, pp. 493–511.

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator

BIOTECHNOLOGY AND BIOENGINEERING, 116(5), 1139–1151.

Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods

BIOTECHNOLOGY JOURNAL, 14(3).

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

NATURE COMMUNICATIONS, 10.

Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells

NATURE BIOTECHNOLOGY, 37(12), 1493-+.

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

FEMS MICROBIOLOGY LETTERS, 366(8).

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

RNA BIOLOGY, 16(4), 404–412.

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

Methods, 143, 48–57.

Advances in CRISPR Technologies for Microbial Strain Engineering

Biotechnology Journal, 13(9), 1800460.

Bacterial Adaptation to the Host's Diet Is a Key Evolutionary Force Shaping Drosophila-Lactobacillus LESymbiosis

CELL HOST & MICROBE, 24(1), 109-+.

CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9

MOLECULAR CELL, 69(5), 893-+.

CRISPR tool puts RNA on the record

Nature, 562(7727), 347–349.

Mathematical Modeling of RNA-Based Architectures for Closed Loop Control of Gene Expression

ACS SYNTHETIC BIOLOGY, 7(5), 1219–1228.

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

MOLECULAR CELL, 69(1), 146-+.

Synthetic Biology Approaches to Engineer Probiotics and Members of the Human Microbiota for Biomedical Applications

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 20, Vol. 20, pp. 277–300.

Advancing the design and delivery of CRISPR antimicrobials

Current Opinion in Biomedical Engineering, 4, 57–64.

Deciphering, Communicating, and Engineering the CRISPR PAM

[Review of ]. JOURNAL OF MOLECULAR BIOLOGY, 429(2), 177–191.

Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems

BIOTECHNOLOGY AND BIOENGINEERING, 114(9), 2137–2141.

Toward a genetic tool development pipeline for host-associated bacteria

Current Opinion in Microbiology, 38, 156–164.

What is the role of circuit design in the advancement of synthetic biology? Part 1 enabling real-world applications and therapeutics

Cell Systems, 4(4), 370–372.

Current and future prospects for CRISPR-based tools in bacteria

[Review of ]. BIOTECHNOLOGY AND BIOENGINEERING, 113(5), 930–943.

Engineering genes with CRISPR-Cas9

Chemical Engineering Progress , 112(9), 36–41.

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

Rethinking the Hierarchy of Sugar Utilization in Bacteria

Beisel, C. L., & Afroz, T. (2016, February). JOURNAL OF BACTERIOLOGY, Vol. 198, pp. 374–376.

The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers

NUCLEIC ACIDS RESEARCH, 44(15), 7385–7394.

Cover Picture: Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing (Angew. Chem. Int. Ed. 41/2015)

Angewandte Chemie International Edition, 54(41), 11877–11877.

Impact of Residual Inducer on Titratable Expression Systems

PLOS ONE, 10(9).

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

NUCLEIC ACIDS RESEARCH, 43(1), 674–681.

Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing

Angewandte Chemie International Edition, 54(41), 12029–12033.

Trade-offs in Engineering Sugar Utilization Pathways for Titratable Control

ACS SYNTHETIC BIOLOGY, 4(2), 141–149.

A CRISPR design for next-generation antimicrobials

GENOME BIOLOGY, Vol. 15.

Bacterial sugar utilization gives rise to distinct single-cell behaviours

MOLECULAR MICROBIOLOGY, 93(6), 1093–1103.

Construction of ligand-responsive microRNAs that operate through inhibition of Drosha processing

Artificial Riboswitches: Methods and Protocols, 1111, 259–267.

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

MOLECULAR CELL, 56(2), 333–339.

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

MBIO, 5(1).

Understanding and exploiting feedback in synthetic biology

CHEMICAL ENGINEERING SCIENCE, 103, 79–90.

Multiple factors dictate target selection by Hfq-binding small RNAs

The EMBO Journal, 31(8), 1961–1974.

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing

Nucleic Acids Research, 39(7), 2981–2994.

Discriminating tastes: physiological contributions of the Hfq-binding small RNA Spot 42 to catabolite repression

RNA Biology, Vol. 8, pp. 1–5.

The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli

Molecular Cell, 41(3), 286–297.

The base pairing RNA Spot 42 participates in a multi-output feedforward loop to help enact catabolite repression in Escherichia coli

Molecular Cell, 41, 1–12.

Base pairing small RNAs and their roles in global regulatory networks

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 34(5), 866–882.

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure [Including Theochem], 3(1).

Design Principles for Riboswitch Function

PLOS COMPUTATIONAL BIOLOGY, 5(4).

Model-guided design of ligand-regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4, 224.

Model‐guided design of ligand‐regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4(1), 224.

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure, 730, 51–58.

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure: THEOCHEM, 730(1-3), 51–58.