Works (53)

2020 journal article

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

METHODS, 172, 42–50.

By: K. Wandera, S. Collins, F. Wimmer, R. Marshall, V. Noireaux & C. Beisel

Source: Web Of Science
Added: April 6, 2020

2020 journal article

Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(7).

By: S. Siedler, M. Rau, S. Bidstrup, J. Vento, S. Aunsbjerg, E. Bosma, L. McNair, C. Beisel, A. Neves

Source: Web Of Science
Added: April 20, 2020

2020 article

Methods for characterizing, applying, and teaching CRISPR-Cas systems

METHODS, Vol. 172, pp. 1–2.

By: C. Beisel

Source: Web Of Science
Added: April 6, 2020

2020 journal article

Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems

PLOS ONE, 15(4).

By: T. Jacobsen, G. Yi, H. Al Asafen, A. Jermusyk, C. Beisel & G. Reeves

Source: Web Of Science
Added: June 22, 2020

2019 journal article

An educational module to explore CRISPR technologies with a cell-free transcription-translation system

SYNTHETIC BIOLOGY, 4(1).

By: D. Collias, R. Marshall, S. Collins, C. Beisel & V. Noireaux

Source: Web Of Science
Added: February 27, 2020

2019 review

Barriers to genome editing with CRISPR in bacteria

[Review of ]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 46(9-10), 1327–1341.

By: J. Vento, N. Crook & C. Beisel

Source: Web Of Science
Added: October 28, 2019

2019 article

CRATES: A one-step assembly method for Class 2 CRISPR arrays

TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - MOLECULAR METHODS, pp. 493–511.

By: C. Liao, R. Slotkowski & C. Beisel

Source: Web Of Science
Added: June 29, 2020

2019 journal article

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator

BIOTECHNOLOGY AND BIOENGINEERING, 116(5), 1139–1151.

By: A. Westbrook, X. Tang, R. Marshall, C. Maxwell, J. Chappell, D. Agrawal, M. Dunlop, V. Noireaux ...

Source: Web Of Science
Added: April 15, 2019

2019 journal article

Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods

BIOTECHNOLOGY JOURNAL, 14(3).

By: R. Leenay, J. Vento, M. Shah, M. Martino, F. Leulier & C. Beisel

Source: Web Of Science
Added: March 18, 2019

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

NATURE COMMUNICATIONS, 10.

By: C. Liao, F. Ttofali, R. Slotkowski, S. Denny, T. Cecil, R. Leenay, A. Keung, C. Beisel

Source: Web Of Science
Added: July 22, 2019

2019 journal article

Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells

NATURE BIOTECHNOLOGY, 37(12), 1493-+.

By: A. Pickar-Oliver, J. Black, M. Lewis, K. Mutchnick, T. Klann, K. Gilcrest, M. Sitton, C. Nelson ...

Source: Web Of Science
Added: December 30, 2019

2019 journal article

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

FEMS MICROBIOLOGY LETTERS, 366(8).

By: T. Jacobsen, C. Liao & C. Beisel

Source: Web Of Science
Added: September 9, 2019

2019 journal article

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

RNA BIOLOGY, 16(4), 404–412.

By: C. Liao, R. Slotkowski, T. Achmedov & C. Beisel

Source: Web Of Science
Added: May 20, 2019

2018 journal article

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

Methods, 143, 48–57.

By: C. Maxwell, T. Jacobsen, R. Marshall, V. Noireaux & C. Beisel

Source: Crossref
Added: February 24, 2020

2018 journal article

Advances in CRISPR Technologies for Microbial Strain Engineering

Biotechnology Journal, 13(9), 1800460.

By: H. Alper & C. Beisel

Source: Crossref
Added: February 24, 2020

2018 journal article

Bacterial adaptation to the host's diet is a key evolutionary force shaping Drosophila-Lactobacillus LESymbiosis

Cell Host & Microbe, 24(1), 109-.

By: M. Martino, P. Joncour, R. Leenay, H. Gervais, M. Shah, S. Hughes, B. Gillet, C. Beisel, F. Leulier

Source: NC State University Libraries
Added: August 6, 2018

2018 journal article

CRISPR RNA-dependent binding and cleavage of endogenous RNAs by the campylobacter jejuni Cas9

Molecular Cell, 69(5), 893-.

By: G. Dugar, R. Leenay, S. Eisenbart, T. Bischler, B. Aul, C. Beisel, C. Sharma

Source: NC State University Libraries
Added: August 6, 2018

2018 journal article

Mathematical modeling of rna-based architectures for closed loop control of gene expression

ACS Synthetic Biology, 7(5), 1219–1228.

By: D. Agrawal, X. Tang, A. Westbrook, R. Marshall, C. Maxwell, J. Lucks, V. Noireaux, C. Beisel, M. Dunlop, E. Franco

Source: NC State University Libraries
Added: August 6, 2018

2018 journal article

Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system

Molecular Cell, 69(1), 146-.

By: R. Marshall, C. Maxwell, S. Collins, T. Jacobsen, M. Luo, M. Begemann, B. Gray, E. January ...

Source: NC State University Libraries
Added: August 6, 2018

2018 journal article

Synthetic biology approaches to engineer probiotics and members of the human microbiota for biomedical applications

Annual Review of Biomedical Engineering, Vol 20, 20, 277–300.

By: J. Bober, C. Beisel & N. Nair

Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

Advancing the design and delivery of CRISPR antimicrobials

Current Opinion in Biomedical Engineering, 4, 57–64.

By: J. Fagen, D. Collias, A. Singh & C. Beisel

Source: Crossref
Added: February 24, 2020

2017 review

Deciphering, communicating, and engineering the CRISPR PAM

[Review of ]. Journal of Molecular Biology, 429(2), 177–191.

By: R. Leenay & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

Short DNA containing chi sites enhances DNA stability and gene expression in E-coli cell-free transcription-translation systems

Biotechnology and Bioengineering, 114(9), 2137–2141.

By: R. Marshall, C. Maxwell, S. Collins, C. Beisel & V. Noireaux

Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

Toward a genetic tool development pipeline for host-associated bacteria

Current Opinion in Microbiology, 38, 156–164.

By: M. Waller, J. Bober, N. Nair & C. Beisel

Source: Crossref
Added: February 24, 2020

2017 journal article

What is the role of circuit design in the advancement of synthetic biology? Part 1 enabling real-world applications and therapeutics

Cell Systems, 4(4), 370–372.

By: Y. Chen, M. Shapiro, W. Ruder, H. Ye, S. Kiani, T. Moon, S. Raman, C. Beisel, C. Barnes

Source: NC State University Libraries
Added: August 6, 2018

2016 review

Current and future prospects for CRISPR-based tools in bacteria

[Review of ]. Biotechnology and Bioengineering, 113(5), 930–943.

By: M. Luo, R. Leenay & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Engineering genes with CRISPR-Cas9

Chemical Engineering Progress , 112(9), 36–41.

By: M. Luo & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Identifying and visualizing functional PAM diversity across CRISPR-cas systems

Molecular Cell, 62(1), 137–147.

By: R. Leenay, K. Maksimchuk, R. Slotkowski, R. Agrawal, A. Gomaa, A. Briner, R. Barrangou, C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Rethinking the hierarchy of sugar utilization in bacteria

Journal of Bacteriology, 198(3), 374–376.

By: C. Beisel & T. Afroz

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers

Nucleic Acids Research, 44(15), 7385–7394.

By: M. Luo, R. Jackson, S. Denny, M. Tokmina-Lukaszewska, K. Maksimchuk, W. Lin, B. Bothner, B. Wiedenheft, C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Cover Picture: Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing (Angew. Chem. Int. Ed. 41/2015)

Angewandte Chemie International Edition, 54(41), 11877–11877.

By: W. Sun, W. Ji, J. Hall, Q. Hu, C. Wang, C. Beisel, Z. Gu

Source: Crossref
Added: February 24, 2020

2015 journal article

Impact of residual inducer on titratable expression systems

PLoS One, 10(9).

By: T. Afroz, M. Luo & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

Nucleic Acids Research, 43(1), 674–681.

By: M. Luo, A. Mullis, R. Leenay & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing

Angewandte Chemie International Edition, 54(41), 12029–12033.

By: W. Sun, W. Ji, J. Hall, Q. Hu, C. Wang, C. Beisel, Z. Gu

Source: Crossref
Added: February 24, 2020

2015 journal article

Trade-offs in engineering sugar utilization pathways for titratable control

ACS Synthetic Biology, 4(2), 141–149.

By: T. Afroz, K. Biliouris, K. Boykin, Y. Kaznessis & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

A CRISPR design for next-generation antimicrobials

Genome Biology, 15(11).

By: C. Beisel, A. Gomaa & R. Barrangou

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Bacterial sugar utilization gives rise to distinct single-cell behaviours

Molecular Microbiology, 93(6), 1093–1103.

By: T. Afroz, K. Biliouris, Y. Kaznessis & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Construction of ligand-responsive microRNAs that operate through inhibition of Drosha processing

Artificial Riboswitches: Methods and Protocols, 1111, 259–267.

By: C. Beisel, R. Bloom & C. Smolke

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Guide RNA functional modules direct Cas9 activity and orthogonality

Molecular Cell, 56(2), 333–339.

By: A. Briner, P. Donohoue, A. Gomaa, K. Selle, E. Slorach, C. Nye, R. Haurwitz, C. Beisel, A. May, R. Barrangou

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems

MBio, 5(1).

By: A. Gomaa, H. Klumpe, M. Luo, K. Selle, R. Barrangou & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Understanding and exploiting feedback in synthetic biology

Chemical Engineering Science, 103, 79–90.

By: T. Afroz & C. Beisel

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Multiple factors dictate target selection by Hfq-binding small RNAs

The EMBO Journal, 31(8), 1961–1974.

By: C. Beisel, T. Updegrove, B. Janson & G. Storz

Source: Crossref
Added: August 28, 2020

2011 journal article

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing

Nucleic Acids Research, 39(7), 2981–2994.

By: C. Beisel CL, C. Yy, . Hoff KG & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2011 article

Discriminating tastes: physiological contributions of the Hfq-binding small RNA Spot 42 to catabolite repression

RNA Biology, Vol. 8, pp. 1–5.

By: C. Beisel & G. Storz

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli

Molecular Cell, 41(3), 286–297.

By: C. Beisel & G. Storz

Source: Crossref
Added: August 28, 2020

2011 journal article

The base pairing RNA Spot 42 participates in a multi-output feedforward loop to help enact catabolite repression in Escherichia coli

Molecular Cell, 41, 1–12.

By: B. Cl & S. G

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Base pairing small RNAs and their roles in global regulatory networks

FEMS Microbiology Reviews, 34(5), 866–882.

By: . Beisel CL & S. G

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure [Including Theochem], 3(1).

By: H. Bayer TS, L. Beisel CL & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Design principles for riboswitch function

PLoS Computational Biology, 5(4), e1000363.

By: . Beisel CL & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Model-guided design of ligand-regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4, 224.

By: B. Beisel CL, . Hoff KG & S. Cd

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Model‐guided design of ligand‐regulated RNAi for programmable control of gene expression

Molecular Systems Biology, 4(1), 224.

By: C. Beisel, T. Bayer, K. Hoff & C. Smolke

Source: Crossref
Added: August 28, 2020

2005 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure, 730, 51–58.

By: D. Beisel CL & R. Pj

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Conformational analysis of gossypol and its derivatives by molecular mechanics

Journal of Molecular Structure: THEOCHEM, 730(1-3), 51–58.

By: C. Beisel, M. Dowd & P. Reilly

Source: Crossref
Added: August 28, 2020