@article{galiakhmetov_shah_lane_davern_proulx_nevzorov_2024, title={Peptoid-based macrodiscs of variable lipid composition for structural studies of membrane proteins by oriented-sample solid-state NMR}, volume={9}, ISSN={["2590-1524"]}, DOI={10.1016/j.yjsbx.2023.100095}, abstractNote={Solid-state Nuclear Magnetic Resonance (NMR) in combination with magnetically aligned discoidal lipid mimics allows for studying the conformations of membrane proteins in planar, lipid-rich bilayer environments and at the physiological temperature. We have recently demonstrated the general applicability of macrodiscs composed of DMPC lipids and peptoid belts, which yield magnetic alignment and NMR spectroscopic resolution comparable or superior to detergent-containing bicelles. Here we report on a considerable improvement in the magnetic alignment and NMR resolution of peptoid-based macrodiscs consisting of a mixture of the zwitterionic and negatively charged lipids (DMPC/DMPG at the 85% to 15% molar ratio). The resulting linewidths are about 30% sharper due to the higher orientational order parameter likely arising from the stabilizing electrostatic repulsion between the discs. Moreover, highly aligned, detergent-free macrodiscs can be formed with a longer-chain lipid, DPPC. Interestingly, the spectra of Pf1 in the two lipid mimetics are almost indistinguishable, which would mean that the overall transmembrane helix tilt might be governed not only by the hydrophobic matching but also possibly by the interactions of the flanking lysine and arginine residues at the membrane interface.}, journal={JOURNAL OF STRUCTURAL BIOLOGY-X}, author={Galiakhmetov, Azamat R. and Shah, Adit A. and Lane, Addison and Davern, Carolynn M. and Proulx, Caroline and Nevzorov, Alexander A.}, year={2024}, month={Jun} }
 @article{davern_proulx_2023, title={Late-Stage Chloride Displacements Enable Access to Peptoids with cis-Inducing Alkylammonium Side Chains}, volume={8}, ISSN={["1523-7052"]}, DOI={10.1021/acs.orglett.3c02393}, abstractNote={The synthesis of peptoids possessing multiple cis-inducing monomers with alkylammonium side chains is reported, where chloropropyl side chains are diversified on a solid support by late-stage SN2 displacements with amines. The conditions were optimized for a wide variety of primary, secondary, and tertiary alkyl amine nucleophiles. We also demonstrated that multiple chloride displacements could be achieved on sequences possessing trans-inducing N-aryl- and N-imino glycine monomers.}, journal={ORGANIC LETTERS}, author={Davern, Carolynn M. and Proulx, Caroline}, year={2023}, month={Aug} }
 @article{galiakhmetov_davern_esteves_awosanya_guthrie_proulx_nevzorov_2022, title={Aligned peptoid-based macrodiscs for structural studies of membrane proteins by oriented-sample NMR}, volume={121}, ISSN={["1542-0086"]}, DOI={10.1016/j.bpj.2022.07.024}, abstractNote={Development of a robust, uniform, and magnetically orientable lipid mimetic will undoubtedly advance solid-state NMR of macroscopically aligned membrane proteins. Here, we report on a novel lipid membrane mimetic based on peptoid belts. The peptoids, composed of 15 residues, were synthesized by alternating N-(2-phenethyl)glycine with N-(2-carboxyethyl)glycine residues at a 2:1 molar ratio. The chemically synthesized peptoids possess a much lower degree of polydispersity versus styrene-maleic acid polymers, thus yielding uniform discs. Moreover, the peptoid oligomers are more flexible and do not require a specific folding, unlike lipoproteins, in order to wrap around the hydrophobic membrane core. The NMR spectra measured for the membrane-bound form of Pf1 coat protein incorporated in this new lipid mimetics demonstrate a higher order parameter and uniform linewidths compared with the conventional bicelles and peptide-based macrodiscs. Importantly, unlike bicelles, the peptoid-based macrodiscs are detergent free.}, number={17}, journal={BIOPHYSICAL JOURNAL}, author={Galiakhmetov, Azamat R. and Davern, Carolynn M. and Esteves, Richard J. A. and Awosanya, Emmanuel O. and Guthrie, Quibria A. E. and Proulx, Caroline and Nevzorov, Alexander A.}, year={2022}, month={Sep}, pages={3263–3270} }
 @article{davern_lowe_rosfi_ison_proulx_2021, title={Submonomer synthesis of peptoids containing trans-inducing N-imino- and N-alkylamino-glycines}, volume={5}, ISSN={["2041-6539"]}, DOI={10.1039/d1sc00717c}, abstractNote={The use of hydrazones as a new type of submonomer in peptoid synthesis is described, giving access to peptoid monomers that are structure-inducing.}, journal={CHEMICAL SCIENCE}, author={Davern, Carolynn M. and Lowe, Brandon D. and Rosfi, Adam and Ison, Elon A. and Proulx, Caroline}, year={2021}, month={May} }