@article{shi_collado_hernandez_2024, title={Improve Cannabis sativa micropropagation through increasing air change rate in photoautotrophic and traditional tissue culture}, volume={372}, ISSN={["1879-1018"]}, DOI={10.1016/j.scienta.2024.113238}, journal={SCIENTIA HORTICULTURAE}, author={Shi, Xiaonan and Collado, Cristian E. and Hernandez, Ricardo}, year={2024}, month={Jun} } @article{collado_hwang_hernandez_2024, title={Supplemental greenhouse lighting increased the water use efficiency, crop growth, and cutting production in Cannabis sativa}, volume={15}, ISSN={["1664-462X"]}, DOI={10.3389/fpls.2024.1371702}, abstractNote={The expanding cannabis production sector faces economic challenges, intensified by freshwater scarcity in the main US production areas. Greenhouse cultivation harnesses sunlight to reduce production costs, yet the impact of greenhouse light levels on crucial production components, such as plant growth, branching, and water use efficiency (WUE), remains poorly understood. This study aimed to assess the effects of combined sunlight and supplemental lighting on the crop’s main production components and leaf gas exchange of Cannabis sativa ‘Suver Haze’ in the vegetative stage. Within a greenhouse, LED lighting provided at intensities of ~150, 300, 500, and 700 µmol m -2 s -1 (18-hour photoperiod), combined with solar radiation, resulted in average daily light integrals of 17.9, 29.8, 39.5, and 51.8 mol m -2 d -1 . Increasing light levels linearly increased biomass, leaf area, and the number of branches per plant and square meter, with respective rates of 0.26 g, 32.5 cm 2 , and 0.41 branches per mole of additional light. As anticipated, crop evapotranspiration increased by 1.8-fold with the increase in light intensity yet crop WUE improved by 1.6-fold when comparing the lowest and highest light treatments. Moreover, water requirements per unit of plant biomass decreased from 0.37 to 0.24 liters per gram when lighting increased from ~18 to 52 mol m -2 d -1 , marking a 35% reduction in evapotranspiration. These results were supported by increments in leaf photosynthesis and WUE with light enhancement. Furthermore, our findings indicate that even 52 mol m -2 d -1 of supplemental lighting did not saturate any of the crop responses to light and can be economically viable for cannabis nurseries. In conclusion, light supplementation strongly enhanced photosynthesis and plant growth while increasing WUE. Additionally, a comprehensive discussion highlights the shared physiological mechanisms governing WUE in diverse plant species and their potential for water conservation under enhanced lighting conditions.}, journal={FRONTIERS IN PLANT SCIENCE}, author={Collado, Cristian E. and Hwang, Seung Jae and Hernandez, Ricardo}, year={2024}, month={Jun} } @article{park_collado_lam_hernandez_2023, title={Flowering Response of Cannabis sativa L. 'Suver Haze' under Varying Daylength-Extension Light Intensities and Durations}, volume={9}, ISSN={["2311-7524"]}, DOI={10.3390/horticulturae9050526}, abstractNote={Daylength-extension lighting (DE) is used in the cannabis industry to increase plant size and produce cuttings by regulating flowering and extending the vegetative stage. Growers have reported incomplete or transitional inflorescences in several Cannabis cultivars even when exposed to long photoperiods. Cannabis sativa L. ‘Suver Haze’ has been reported to develop incomplete inflorescences in North Carolina nurseries using photoperiods of 15 h. The objectives of this study were to investigate the required light intensity and photoperiod to inhibit the flowering of ‘Suver Haze’. In Experiment 1, DE of 1.0, 2.5, 5.8, and 10.3 µmol·m−2·s−1 of photosynthetic photon flux density from incandescent lamps were used to extend the photoperiod of ‘Suver Haze’ from 9 to 15 h. A 9 h photoperiod control was included. The results showed that all DE treatments stopped the full transition to flowering compared to the control; however, all DE-treated plants showed the presence of incomplete inflorescences. In Experiment 2, three photoperiod treatments of 15 h, 18 h, and 21 h were tested. ‘Suver Haze’ under 18 h and 21 h photoperiods did not develop incomplete inflorescences in contrast to plants in 15 h photoperiod. Therefore, a light intensity of at least 1.0 µmol·m−2·s−1 PPFD and an 18 h photoperiod are required to prevent incomplete inflorescences and flowering of ‘Suver Haze’.}, number={5}, journal={HORTICULTURAE}, author={Park, Jongseok and Collado, Cristian E. E. and Lam, Vu Phong and Hernandez, Ricardo}, year={2023}, month={Apr} } @article{collado_hernandez_2022, title={Effects of Light Intensity, Spectral Composition, and Paclobutrazol on the Morphology, Physiology, and Growth of Petunia, Geranium, Pansy, and Dianthus Ornamental Transplants}, volume={41}, ISSN={["1435-8107"]}, url={https://doi.org/10.1007/s00344-021-10306-5}, DOI={10.1007/s00344-021-10306-5}, number={2}, journal={JOURNAL OF PLANT GROWTH REGULATION}, publisher={Springer Science and Business Media LLC}, author={Collado, Cristian E. and Hernandez, Ricardo}, year={2022}, month={Feb}, pages={461–478} }