Charles E. Melvin

Works (4)

Updated: July 5th, 2023 15:37

2020 chapter

BioVision Tracker: A semi-automated image analysis software for spatiotemporal gene expression tracking in Arabidopsis thaliana

In Methods in Cell Biology (Vol. 160, pp. 419–436).

By: E. Buckner n, I. Madison n, C. Melvin n, T. Long n , R. Sozzani  n & C. Williams n 

Contributors: E. Buckner n, I. Madison n, C. Melvin n, T. Long n , R. Sozzani n  & C. Williams n 

MeSH headings : Arabidopsis / genetics; Automation; Gene Expression Regulation, Plant; Image Processing, Computer-Assisted; Plant Roots / anatomy & histology; Software; Time Factors
TL;DR: A procedure for analyzing 3D microscopy images to track spatiotemporal gene expression signals using the MATLAB-based BioVision Tracker software, which requires either a fluorescence image or a brightfield image to analyze root geometry and afluorescence image to capture and track temporal changes in gene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: June 17, 2020

2020 chapter

MAGIC: Live imaging of cellular division in plant seedlings using lightsheet microscopy

In Methods in Cell Biology (Vol. 160, pp. 405–418).

By: I. Madison n, C. Melvin n, E. Buckner n, C. Williams n , R. Sozzani  n & T. Long n 

Contributors: I. Madison n, C. Melvin n, E. Buckner n, C. Williams n , R. Sozzani n  & T. Long n 

MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; Cell Division; Imaging, Three-Dimensional; Microscopy, Fluorescence / methods; Seedlings / cytology; Seedlings / growth & development
TL;DR: This chapter describes a detailed method for preparing and imaging Arabidopsis thaliana seedlings for lightsheet microscopy via a Multi-Sample Imaging Growth Chamber (MAGIC), which allows simultaneous imaging of at least four samples. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: June 17, 2020

2017 journal article

Dose-Duration Reciprocity for G protein activation: Modulation of kinase to substrate ratio alters cell signaling

PLOS ONE, 12(12).

By: K. Liao*, C. Melvin n, R. Sozzani  n, R. Jones *, T. Elston * & A. Jones *

MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; GTP-Binding Proteins / metabolism; Genotype; Phosphorylation; Phosphotransferases / metabolism; Signal Transduction; Substrate Specificity
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Multi-sample Arabidopsis Growth and Imaging Chamber (MAGIC) for long term imaging in the ZEISS Lightsheet Z.1

Developmental Biology, 419(1), 19–25.

By: M. Luis Balaguer n, M. Ramos-Pezzotti n, M. Rahhal n, C. Melvin n, E. Johannes  n, T. Horn n , R. Sozzani  n

author keywords: Arabidopsis root; Light sheet; 3D printing; Time-lapse imaging
MeSH headings : Arabidopsis / ultrastructure; Arabidopsis Proteins / analysis; Cell Division; Computer-Aided Design; Cyclin B / analysis; Equipment Design; Green Fluorescent Proteins / analysis; Image Processing, Computer-Assisted / methods; Microscopy, Fluorescence / instrumentation; Microscopy, Fluorescence / methods; Plant Roots / ultrastructure; Printing, Three-Dimensional; Recombinant Fusion Proteins / analysis; Time-Lapse Imaging / instrumentation; Time-Lapse Imaging / methods
TL;DR: A Multi-sample Arabidopsis Growth and Imaging Chamber (MAGIC) is developed that provides near-physiological imaging conditions and allows high-throughput time-course imaging experiments in the ZEISS Lightsheet Z.1, and its imaging capabilities are illustrated by following cell divisions, as an indicator of plant growth and development, over prolonged time periods. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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