Works (115)

Updated: April 3rd, 2024 19:43

2024 article

Structural determination of a full-length plant cellulose synthase informed by experimental and in silico methods

Kwansa, A. L., Singh, A., Williams, J. T., Haigler, C. H., Roberts, A. W., & Yingling, Y. G. (2024, January 6). CELLULOSE, Vol. 1.

By: A. Kwansa n, A. Singh n, J. Williams n, C. Haigler n, A. Roberts* & Y. Yingling n

author keywords: Plant cellulose synthesis; Protein homology model; Molecular dynamics simulation; Hydrogen bonding; Gossypium hirsutum
Sources: Web Of Science, NC State University Libraries
Added: January 16, 2024

2022 article

Efficient imaging and computer vision detection of two cell shapes in young cotton fibers

Graham, B. P., Park, J., Billings, G. T., Hulse-Kemp, A. M., Haigler, C. H., & Lobaton, E. (2022, November 26). APPLICATIONS IN PLANT SCIENCES, Vol. 11.

By: B. Graham n, J. Park n, G. Billings n, A. Hulse-Kemp n, C. Haigler n & E. Lobaton n

author keywords: cotton diversity; fiber morphogenesis; Gossypium barbadense; Gossypium hirsutum; light microscopy; machine learning
TL;DR: Improved semi‐automated imaging methods for young cotton fibers and a novel machine learning algorithm for the rapid detection of tapered or hemisphere fibers in homogeneous or mixed populations are developed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: November 28, 2022

2022 journal article

Leveraging National Germplasm Collections to Determine Significantly Associated Categorical Traits in Crops: Upland and Pima Cotton as a Case Study

FRONTIERS IN PLANT SCIENCE, 13.

By: D. Restrepo-Montoya n, A. Hulse-Kemp n, J. Scheffler*, C. Haigler n, L. Hinze*, J. Love*, R. Percy*, D. Jones*, J. Frelichowski*

author keywords: trait association; categorical data; cotton; crop germplasm; breeding
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 31, 2022

2021 journal article

Microtubules exert early, partial, and variable control of cotton fiber diameter

PLANTA, 253(2).

By: B. Graham n & C. Haigler n

author keywords: Gossypium hirsutum; Gossypium barbadense; Colchicine; Taxol; Ovule culture; Immunofluorescence; Plant cell growth; Plant cell shape control
MeSH headings : Cotton Fiber; Gene Expression Regulation, Plant; Gossypium; Microtubules / metabolism; Phenotype
TL;DR: Overall, the results demonstrate cell-type-specific mechanisms regulating fiber expansion within 80 µm of the apex, with variation in the impact of microtubules between tip types and over developmental time. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 22, 2021

2021 journal article

Phenotypic effects of changes in the FTVTxK region of an Arabidopsis secondary wall cellulose synthase compared with results from analogous mutations in other isoforms

PLANT DIRECT, 5(8).

By: J. Burris n, M. Makarem*, E. Slabaugh n, A. Chaves*, E. Pierce n, J. Lee*, S. Kiemle*, A. Kwansa n ...

author keywords: AtCesA7; cellulose synthesis complex; genetic complementation; irx3-1 mutant; PpCesA5; protein engineering; sum frequency generation spectroscopy
TL;DR: The cumulative results show that the FTVTxK region is important for the function of an angiosperm secondary wall CesA as well as widely divergent primary wall CesAs, while differences in complementation results between isomers may reflect functional differences that can be explored in further work. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2021

2020 journal article

In silico structure prediction of full-length cotton cellulose synthase protein (GhCESA1) and its hierarchical complexes

Cellulose, 27(10), 5597–5616.

By: A. Singh n, A. Kwansa n, H. Kim n, J. Williams n, H. Yang*, N. Li n, J. Kubicki*, A. Roberts*, C. Haigler n, Y. Yingling n

author keywords: Plant; Cellulose synthesis complex; Homotrimer; Protein structure prediction; Molecular dynamics simulation
TL;DR: A 3D atomic-resolution model of a full-length cotton CESA (GhCESA1) structure is presented that indicates that there are similarities to BcsA bacterial cellulose synthase, such as the transmembrane topology and the internalization of conserved catalytic motifs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries, ORCID
Added: May 1, 2020

2019 journal article

Cultures of Gossypium barbadense cotton ovules offer insights into the microtubule-mediated control of fiber cell expansion

Planta, 249(5), 1551–1563.

By: E. Pierce n, B. Graham n, M. Stiff n, J. Osborne n & C. Haigler n

author keywords: Colchicine; Cotton fiber; Cytoskeleton; Fluridone; Ovule culture; Plant cell growth
MeSH headings : Cotton Fiber; Cytoskeleton / drug effects; Cytoskeleton / metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant / drug effects; Gossypium / cytology; Gossypium / drug effects; Gossypium / metabolism; Microtubules / drug effects; Microtubules / metabolism; Pyridones / pharmacology
TL;DR: A novel method for culturing ovules of G. barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion, which supported the zonal control of cell expansion in one type ofG. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 6, 2019

2018 journal article

Cellulose synthase "class specific regions' are intrinsically disordered and functionally undifferentiated

JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 60(6), 481–497.

By: T. Scavuzzo-Duggan*, A. Chaves*, A. Singh n, L. Sethaphong n, E. Slabaugh n, Y. Yingling n, C. Haigler n, A. Roberts*

Contributors: T. Scavuzzo-Duggan*, A. Chaves*, A. Singh n, L. Sethaphong n, E. Slabaugh n, Y. Yingling n, C. Haigler n, A. Roberts*

MeSH headings : Amino Acid Sequence; Bryopsida / enzymology; Cellulose / metabolism; Gene Knockout Techniques; Genetic Complementation Test; Genetic Vectors / metabolism; Glucosyltransferases / chemistry; Glucosyltransferases / classification; Intrinsically Disordered Proteins / chemistry; Intrinsically Disordered Proteins / metabolism; Isoenzymes / chemistry; Isoenzymes / metabolism; Models, Molecular; Phylogeny
TL;DR: Sequence analysis and computational modeling showed that the CSR is intrinsically disordered and contains predicted molecular recognition features, consistent with a possible role in CESA oligomerization and explaining the evolution of class-specific sequences without selection for class- specific function. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2018 journal article

Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity

Plant Direct, 2(7), e00061.

By: J. Hill*, A. Hill*, A. Roberts*, C. Haigler n & M. Tien*

author keywords: Arabidopsis thaliana; cellulose synthase; chimera; class specificity; domain swap; protein interaction; secondary cell wall
TL;DR: Results indicate that the central domain, as well as the N‐ and C‐terminal regions, contributes to class‐specific function variously in Arabidopsis CESA4, CESA7, and CESA8. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2018 journal article

Structure/function relationships in the rosette cellulose synthesis complex illuminated by an evolutionary perspective

Cellulose, 26(1), 227–247.

By: C. Haigler n & A. Roberts*

author keywords: Cellulose synthase; Constructive neutral evolution; Cellulose microfibril; Protein structure; Rosette cellulose synthesis complex
TL;DR: Major advances in understanding CESA and CSC structure/function relationships since 2013 are reviewed, when ground-breaking insights about the structure of cellulose synthases in bacteria and plants were published. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: March 4, 2019

2018 journal article

Two types of cellulose synthesis complex knit the plant cell wall together

Proceedings of the National Academy of Sciences, 115(27), 6882–6884.

By: C. Haigler n

MeSH headings : Arabidopsis; Cell Wall; Cellulose; Gene Expression Regulation, Plant; Glucosyltransferases; Plant Cells
TL;DR: Key insights are generated into the dynamic regulation of the cellulose synthase (CESA) enzyme family during the transition between the synthesis of the primary cell wall (PCW), which surrounds all expanding plant cells, and the SCW, which confers specialized functions to some cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Modifications to a LATE MERISTEM IDENTITY1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.)

Proceedings of the National Academy of Sciences of the United States of America, 114(1), E57–66.

By: R. Andres n, V. Coneva*, M. Frank*, J. Tuttle n, L. Samayoa n, S. Han n, B. Kaur n, L. Zhu n ...

TL;DR: It is shown that the major leaf shapes of cotton at the L-D1 locus are controlled by a HD-Zip transcription factor most similar to Late Meristem Identity1 (LMI1) gene, and that sub-okra is the ancestral leaf shape of tetraploid cotton and normal is a derived mutant allele that came to predominate and define the leafshape of cultivated cotton. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2016 journal article

A Structural Study of CESA1 Catalytic Domain of Arabidopsis Cellulose Synthesis Complex: Evidence for CESA Trimers

Plant Physiology, 170(1), 123–135.

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Catalytic Domain; Cellulose / biosynthesis; Cellulose / metabolism; Escherichia coli / genetics; Glucosyltransferases / chemistry; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Microscopy, Electron, Transmission; Models, Molecular; Multiprotein Complexes / genetics; Multiprotein Complexes / metabolism; Protein Multimerization; Protein Structure, Secondary; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Scattering, Small Angle; X-Ray Diffraction
TL;DR: The “hexamer of trimers” model for the rosette cellulose synthesis complex that synthesizes an 18-chain cellulose microfibril as its fundamental product strongly supports the number of cellulose synthase proteins in this large multisubunit transmembrane protein complex. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2016 chapter

Biosynthesis and Assembly of Cellulose

In Molecular Cell Biology of the Growth and Differentiation of Plant Cells (pp. 120–138).

By: C. Haigler*, J. Davis, E. Slabaugh & J. Kubicki

Sources: Crossref, NC State University Libraries
Added: October 14, 2020

2016 journal article

Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex

Scientific Reports, 6(1).

By: B. Nixon*, K. Mansouri n, A. Singh n, J. Du*, J. Davis n, J. Lee n, E. Slabaugh n, V. Vandavasi* ...

Contributors: B. Nixon*, K. Mansouri n, A. Singh n, J. Du*, J. Davis n, J. Lee n, E. Slabaugh n, V. Vandavasi* ...

MeSH headings : Cellulose / biosynthesis; Cellulose / chemistry; Glucosyltransferases / chemistry; Models, Molecular; Plant Proteins / chemistry; Protein Domains; Protein Folding; Protein Structure, Quaternary
TL;DR: Improved transmission electron microscopy images and image averages of the rosette-type CSC are obtained, revealing the frequent triangularity and average cross-sectional area in the plasma membrane of its individual lobes and multifaceted data support a rosettes CSC with 18 CESAs that mediates the synthesis of a fundamental microfibril composed of 18 glucan chains. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2016 journal article

Cotton fiber tips have diverse morphologies and show evidence of apical cell wall synthesis

SCIENTIFIC REPORTS, 6.

By: M. Stiff n & C. Haigler n

MeSH headings : Cell Wall / metabolism; Cell Wall / ultrastructure; Cotton Fiber; Cryoelectron Microscopy; Epitopes; Flowers / cytology; Flowers / physiology; Glucans / immunology; Glucans / metabolism; Gossypium / cytology; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Pectins / immunology; Pectins / metabolism; Plant Cells / metabolism; Polysaccharides / metabolism; Xylans / immunology; Xylans / metabolism
TL;DR: Diverse cotton fiber tip morphologies and support primary wall synthesis occurring at the apex and discrete distal regions of the tip are revealed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 chapter

Cotton Fiber Biotechnology: Potential Controls and Transgenic Improvement of Elongation and Cell Wall Thickening

In K. Ramawat & M. Ahuja (Eds.), Fiber Plants (pp. 127–153).

By: M. Stiff n, J. Tuttle n, B. Graham n & C. Haigler n

Ed(s): K. Ramawat & M. Ahuja

author keywords: Cotton fiber development; Fiber improvement; Fiber quality; Primary cell wall; Secondary cell wall
TL;DR: This chapter presents a brief review of cotton fiber development with a focus on processes affecting elongation and cell wall thickening, and highlights selected genetic engineering experiments that have resulted in improved cotton fiber quality. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2016 journal article

Modifications to a LATE MERISTEM IDENTITY1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.)

Proceedings of the National Academy of Sciences, 114(1), E57–E66.

By: R. Andres n, V. Coneva*, M. Frank*, J. Tuttle n, L. Samayoa n, S. Han n, B. Kaur n, L. Zhu n ...

Contributors: R. Andres n, V. Coneva*, M. Frank*, J. Tuttle n, L. Samayoa n, S. Han n, B. Kaur n, L. Zhu n ...

author keywords: cotton; leaf shape; okra; gene cloning
MeSH headings : Amino Acid Sequence / genetics; Frameshift Mutation / genetics; Gene Expression Regulation, Plant; Genes, Plant / genetics; Gossypium / genetics; Gossypium / physiology; Plant Leaves / genetics; Plant Leaves / physiology; Promoter Regions, Genetic / genetics; Transcription Factors / genetics
TL;DR: The results indicate that subokra is the ancestral leaf shape of tetraploid cotton that gave rise to the okra allele and that normal is a derived mutant allele that came to predominate and define the leafshape of cultivated cotton. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 30, 2019

2016 journal article

Prediction of the structures of the plant-specific regions of vascular plant cellulose synthases and correlated functional analysis

Cellulose, 23(1), 145–161.

By: L. Sethaphong n, J. Davis n, E. Slabaugh n, A. Singh n, C. Haigler n & Y. Yingling n

Contributors: Y. Yingling n, C. Haigler n, A. Singh n, E. Slabaugh n, J. Davis n & L. Sethaphong n

author keywords: Arabidopsis thaliana; Cellulose synthesis; Computational protein structure prediction; Isoform specificity; Mutant complementation
TL;DR: Modeling of the mutant P-CR of Atcesa8R362K (fra6) suggested that changes in local structural stability and surface electrostatics may cause the mutant phenotype, and results provide structural clues about the function of plant-specific regions of CESA. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2016 journal article

Structural Studies of Plant CESA Support Eighteen CESAs in the Plant CSC

Biophysical Journal, 110(3), 27a.

By: V. Vandavasi*, D. Putnam*, Q. Zhang*, L. Petridis*, W. Heller*, B. Nixon*, C. Haigler n, U. Kalluri* ...

TL;DR: These studies are the first experimental evidence that CESA trimers form the lobes of rosette CSCs providing strong support for the hexamer of trimers model that synthesizes an 18-chain cellulose microfibril as the fundamental product of cellulose synthesis in plants. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2015 journal article

Comprehensive analysis of cellulose content, crystallinity, and lateral packing in Gossypium hirsutum and Gossypium barbadense cotton fibers using sum frequency generation, infrared and Raman spectroscopy, and X-ray diffraction

Cellulose, 22(2), 971–989.

By: C. Lee*, K. Kafle*, D. Belias*, Y. Park*, R. Glick n, C. Haigler n, S. Kim*

author keywords: Sum frequency generation; Infrared spectroscopy; Raman spectroscopy; Cotton fiber development; Plant cell wall; X-ray diffraction
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

Metabolomic and transcriptomic insights into how cotton fiber transitions to secondary wall synthesis, represses lignification, and prolongs elongation

BMC Genomics, 16(1).

By: J. Tuttle n, G. Nah*, M. Duke*, D. Alexander*, X. Guan*, Q. Song*, Z. Chen*, B. Scheffler*, C. Haigler n

author keywords: Ascorbate; Cell elongation; Cell wall synthesis; Cotton fiber development; Gossypium; Lignification; Reactive oxygen species; Metabolomics; RNA Seq transcriptomics
MeSH headings : Carbohydrate Metabolism / genetics; Cell Wall / genetics; Cotton Fiber / methods; Gene Expression Profiling; Gene Expression Regulation, Plant / genetics; Genes, Plant / genetics; Glucosyltransferases / genetics; Gossypium / genetics; Metabolome / genetics; Metabolomics / methods; Transcriptome / genetics
TL;DR: The parallel data on deep-sequencing transcriptomics and non-targeted metabolomics for two genotypes of single-celled cotton fiber showed that a discrete developmental stage of transitional cell wall remodeling occurs before secondary wall cellulose synthesis begins, and implicated enhanced capacity to manage reactive oxygen species through the ascorbate-glutathione cycle as a positive contributor to fiber length. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

The valine and lysine residues in the conserved FxVTxK motif are important for the function of phylogenetically distant plant cellulose synthases

Glycobiology, 26(5), 509–519.

By: E. Slabaugh n, T. Scavuzzo-Duggan*, A. Chaves*, L. Wilson*, C. Wilson n, J. Davis n, D. Cosgrove*, C. Anderson*, A. Roberts*, C. Haigler n

author keywords: cellulose synthesis; gametophore formation; phenotypic complementation; root elongation; site-directed mutagenesis
MeSH headings : Amino Acid Motifs; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Bryopsida / enzymology; Bryopsida / genetics; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Lysine / genetics; Lysine / metabolism; Valine / genetics; Valine / metabolism
TL;DR: The results support the possibility that the FxVTxK region functions similarly in CESA and BcsA, and show that Physcomitrella provides an efficient model for assessing the effects of engineered CESA mutations affecting primary cell wall synthesis and that diverse testing systems can lead to nuanced insights into CESA structure-function relationships. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 chapter

Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton

In Methods in Molecular Biology (Vol. 1287, pp. 219–234).

By: J. Tuttle n, C. Haigler n & D. Robertson

author keywords: Virus-induced gene silencing; VIGS; Cotton fiber; GFP; Silencing; Semiquantitative RT-PCR
MeSH headings : Agrobacterium / genetics; Agrobacterium / physiology; Agrobacterium / virology; Cotton Fiber; Cotyledon / genetics; Cotyledon / growth & development; Cotyledon / microbiology; Geminiviridae / genetics; Gene Silencing; Gene Transfer Techniques; Genes, Plant; Genetic Vectors / genetics; Gossypium / genetics; Gossypium / growth & development; Gossypium / microbiology; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Plants, Genetically Modified / growth & development; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR: The use of Green Fluorescent Protein (GFP) is described as a marker for identifying silenced tissues in reproductive tissues, a procedure that requires the use of transgenic plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2014 journal article

Cellulose synthases: new insights from crystallography and modeling

Trends in Plant Science, 19(2), 99–106.

By: E. Slabaugh n, J. Davis n, C. Haigler n, Y. Yingling n & J. Zimmer*

Contributors: J. Zimmer*, Y. Yingling n, C. Haigler n, J. Davis n & E. Slabaugh n

MeSH headings : Catalytic Domain; Crystallography; Glucosyltransferases / chemistry; Models, Molecular; Plant Proteins / chemistry
TL;DR: It is shown how the structures of CelS proteins can be used to generate hypotheses for experiments testing mechanisms of glucan synthesis and translocation in plant CelS, and how these structures impact the understanding of the synthesis of cellulose and other extracellular polysaccharides. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2014 journal article

Changes in the cell wall and cellulose content of developing cotton fibers investigated by FTIR spectroscopy

Carbohydrate Polymers, 100, 9–16.

By: N. Abidi*, L. Cabrales* & C. Haigler n

author keywords: Cellulose; FTIR; Cotton; Fiber development; Maturity; Cell wall
MeSH headings : Cell Wall / metabolism; Cellulose / metabolism; Cotton Fiber; Gossypium / cytology; Gossypium / metabolism; Spectroscopy, Fourier Transform Infrared
TL;DR: Changes in cellulose during cotton fiber growth and development were identified through changes in numerous vibrations within the spectra and the intensity of the vibration bands at 667 and 897 cm(-1) correlated with percentage of cellulose analyzed chemically. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2014 journal article

Computational and genetic evidence that different structural conformations of a non-catalytic region affect the function of plant cellulose synthase

Journal of Experimental Botany, 65(22), 6645–6653.

Contributors: E. Slabaugh n, L. Sethaphong n, C. Xiao*, J. Amick n, C. Anderson*, C. Haigler n, Y. Yingling n

author keywords: Cellulose biosynthesis; CESA; genetic complementation; protein structural modelling; rsw1 mutant; transmembrane helix
MeSH headings : Amino Acid Sequence; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Biocatalysis; Computational Biology; Glucosyltransferases / chemistry; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Molecular Sequence Data; Mutant Proteins / metabolism; Mutation; Phenotype; Protein Structure, Secondary; Recombinant Fusion Proteins / metabolism; Sequence Alignment; Structure-Activity Relationship
TL;DR: Computational modelling of peptide structure, genetic complementation in Arabidopsis thaliana, and confocal microscopy provide evidence that a region between two transmembrane helices may adopt two predominant structural conformations that affect the function of plant cellulose synthase. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2014 journal article

Molecular Modeling and Imaging of Initial Stages of Cellulose Fibril Assembly: Evidence for a Disordered Intermediate Stage

PLoS ONE, 9(4), e93981.

By: C. Haigler n, M. Grimson*, J. Gervais*, N. Le Moigne*, H. Höfte*, B. Monasse*, P. Navard*

Ed(s): M. Barbosa

MeSH headings : Asteraceae; Cell Membrane / chemistry; Cell Wall / enzymology; Cellulose / chemistry; Computer Simulation; Freeze Fracturing; Glucans / chemistry; Glucose / chemistry; Hydrogen Bonding; Models, Molecular; Molecular Dynamics Simulation; Motion
TL;DR: The data support the possibility that a zone of uncrystallized chains on the plasma membrane surface buffers the predicted variable rates of cellulose polymerization from multiple catalytic subunits within the CSC and acts as a flexible hinge allowing the horizontal alignment of the crystalline cellulose fibrils relative to the cell wall. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2013 journal article

Cotton Fiber Cell Walls of Gossypium hirsutum and Gossypium barbadense Have Differences Related to Loosely-Bound Xyloglucan

PLoS ONE, 8(2), e56315.

By: U. Avci*, S. Pattathil*, B. Singh n, V. Brown*, M. Hahn* & C. Haigler n

Ed(s): J. Zhang

MeSH headings : Cell Wall / metabolism; Cotton Fiber; Glucans / metabolism; Glycomics; Gossypium / cytology; Gossypium / metabolism; Hydrolysis; Species Specificity; Xylans / metabolism
TL;DR: Comparison of cell wall chemistry over the time course of fiber development in Gh and Gossypium barbadense, the two most important commercial cotton species, when plants were grown in parallel in a highly controlled greenhouse provides a rich picture of the similarities and differences in the cell wall structure of the twomost important cotton species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2013 journal article

Tertiary model of a plant cellulose synthase

Proceedings of the National Academy of Sciences, 110(18), 7512–7517.

By: L. Sethaphong*, C. Haigler n, J. Kubicki*, J. Zimmer*, D. Bonetta*, S. DeBolt*, Y. Yingling*

Contributors: Y. Yingling*, S. DeBolt*, D. Bonetta*, J. Zimmer*, J. Kubicki*, C. Haigler n, L. Sethaphong*

author keywords: rosette cellulose synthase complex; molecular modeling; protein structure prediction; GlycosylTransferase Family 2; beta-1,4-glucan polymerization
MeSH headings : Arabidopsis / enzymology; Bacteria / enzymology; Computational Biology; Cytosol / enzymology; Glucosyltransferases / chemistry; Glucosyltransferases / genetics; Gossypium / enzymology; Models, Molecular; Mutation / genetics; Phenotype; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary
TL;DR: The predicted tertiary structure of cotton CESA provides a platform for the biochemical engineering of plant CESAs and supports the importance of the plant-conserved region and/or class-specific region in CESA oligomerization to form the multimeric cellulose–synthesis complexes that are characteristic of plants. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2012 journal article

Cotton fiber: a powerful single-cell model for cell wall and cellulose research

Frontiers in Plant Science, 3.

By: C. Haigler n, L. Betancur, M. Stiff n & J. Tuttle n

author keywords: cellulose; cotton fiber; elongation; Gossypium; pectin; transition stage; virus-induced gene silencing; xyloglucan
TL;DR: Prior contributions of cotton fiber to building fundamental knowledge about cell walls will be summarized and the dynamic changes in cell wall polymers throughout cotton fiber differentiation will be described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Crossref, NC State University Libraries
Added: October 13, 2020

2012 journal article

Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Plant Methods, 8(1), 27.

By: J. Tuttle n, C. Haigler n & D. Robertson n

author keywords: Cotton; VIGS; Virus-induced gene silencing; Cotton leaf crumple virus; Agroinoculation; Particle inflow gun; Bombardment; Gossypium
TL;DR: The adaptation of a virus-induced gene silencing vector for cotton from the bipartite geminivirusCotton leaf crumple virus for delivery by Agrobacterium is described and the construction of two low-cost particle inflow guns are described to extend the versatility of CLCrV-based VIGS for analyzing gene function in cotton. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Moss cell walls: structure and biosynthesis

Frontiers in Plant Science, 3.

By: A. Roberts*, E. Roberts* & C. Haigler n

author keywords: cell wall; polysaccharide; cellulose; cellulose synthesis complex; glycosyl transferase; moss; Physcomitrella patens
TL;DR: The genome sequence of the moss Physcomitrella patens has stimulated new research examining the cell wall polysaccharides of mosses and the glycosyl transferases that synthesize them as a means to understand fundamental processes of cell wall biosynthesis and plant cell wall evolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: October 13, 2020

2012 chapter

Recent Advances in Cotton Fiber Development

In D. M. Oosterhuis & J. T. Cothren (Eds.), Flowering and Fruiting in Cotton (pp. 163–192). Retrieved from (http://www.cotton.org/foundation/fandfcontents.cfm), Cotton Physiology Book Series, The Cotton Foundation, Cordova TN

By: M. Stiff & C. Haigler

Ed(s): D. Oosterhuis & J. Cothren

Source: NC State University Libraries
Added: April 24, 2021

2012 journal article

Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres

Nature, 492(7429), 423–427.

By: A. Paterson*, J. Wendel*, H. Gundlach*, H. Guo*, J. Jenkins*, D. Jin*, D. Llewellyn*, K. Showmaker* ...

MeSH headings : Alleles; Biological Evolution; Cacao / genetics; Chromosomes, Plant / genetics; Cotton Fiber; Diploidy; Gene Duplication / genetics; Genes, Plant / genetics; Genome, Plant / genetics; Gossypium / classification; Gossypium / genetics; Molecular Sequence Annotation; Phylogeny; Polyploidy; Vitis / genetics
TL;DR: It is shown that an abrupt five- to sixfold ploidy increase approximately 60 million years (Myr) ago, and allopolyploidy reuniting divergent Gossypium genomes approximately 1–2 Myr ago, conferred about 30–36-fold duplication of ancestral angiosperm genes in elite cottons, genetic complexity equalled only by Brassica among sequenced angiosperms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2011 journal article

Perturbation of Wood Cellulose Synthesis Causes Pleiotropic Effects in Transgenic Aspen

Molecular Plant, 4(2), 331–345.

By: C. Joshi*, S. Thammannagowda*, T. Fujino*, J. Gou*, U. Avci n, C. Haigler n, L. McDonnell*, S. Mansfield* ...

author keywords: Aspen; cellulose synthesis; transgenic trees; xylem development; cell wall; lignin; irregular xylem; growth; crystallinity
MeSH headings : Cellulose / metabolism; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Lignin / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Plants, Genetically Modified / metabolism; Populus / genetics; Populus / growth & development; Populus / metabolism
TL;DR: The fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees is demonstrated. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2011 journal article

Starch Self-Processing in Transgenic Sweet Potato Roots Expressing a Hyperthermophilic alpha-Amylase

BIOTECHNOLOGY PROGRESS, 27(2), 351–359.

By: M. Santa-Maria n, C. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n & B. Sosinski n

author keywords: hyperthermophilic enzymes; starch conversion; transgenic plants; sweet potato; biofuels
MeSH headings : Crops, Agricultural / genetics; Hot Temperature; Ipomoea batatas / genetics; Ipomoea batatas / metabolism; Plant Roots / metabolism; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / metabolism; Southeastern United States; Starch / metabolism; Thermotoga maritima / enzymology; alpha-Amylases / genetics; alpha-Amylases / physiology
TL;DR: The results presented here demonstrate that engineering plants with hyperthermophilic glycoside hydrolases can facilitate cost effective starch conversion to fermentable sugars and the use of sweet potato as an alternative near‐term energy crop should be considered. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

The effect of calcium on early fiber elongation in cotton ovule culture

Journal of Cotton Science, 15, 154–161. http://journal.cotton.org/journal/2011-15/2/154.cfm

By: E. Taliercio & C. Haigler

Source: NC State University Libraries
Added: April 24, 2021

2010 journal article

3D volumes constructed from pixel-based images by digitally clearing plant and animal tissue

Journal of Microscopy, 240(2), 122–129.

By: D. Livingston n, T. Tuong n, S. Gadi n, C. Haigler n, R. Gelman & J. Cullen n

author keywords: Adobe After Effects; biopsy; frozen plant; in situ hybridization; light microscopy; liver; lung; paraffin; radiofrequency ablation; tumour; 3D reconstruction
MeSH headings : Animals; Avena / anatomy & histology; Imaging, Three-Dimensional / methods; Liver / anatomy & histology; Marmota; Mice; Pulmonary Veins / anatomy & histology
TL;DR: Three‐dimensional volumes of the crown of an oat plant showing internal responses to a freezing treatment, a sample of a hepatocellular carcinoma from a woodchuck liver that had been heat‐treated with computer‐guided radiofrequency ablation to induce necrosis in the central portion of the tumour, and several features of a samples of mouse lung are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2010 journal article

Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Physiological and Molecular Plant Pathology, 75(1-2), 13–22.

By: A. Idris*, J. Tuttle n, D. Robertson n, C. Haigler n & J. Brown*

author keywords: Begomovirus; Cotton breeding; Cotton genetics; Genetic improvement; Geminivirus; Magnesium chelatase marker; Viral episome
TL;DR: A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous genesilencing in cotton of varied genetic backgrounds, indicating successful phloem invasion in all cultivars tested. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2010 journal article

Gene expression in developing fibres of Upland cotton (Gossypium hirsutum L.) was massively altered by domestication

BMC Biology, 8(1), 139.

By: R. Rapp*, C. Haigler n, L. Flagel*, R. Hovav*, J. Udall* & J. Wendel*

MeSH headings : Breeding / methods; Cotton Fiber; Gene Expression Regulation, Developmental / genetics; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks / genetics; Gossypium / genetics; Gossypium / metabolism; Models, Biological; Selection, Genetic
TL;DR: It is shown that artificial selection during crop domestication can radically alter the transcriptional developmental network of even a single-celled structure, affecting nearly a quarter of the genes in the genome. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2010 journal article

Phylogenetically Distinct Cellulose Synthase Genes Support Secondary Wall Thickening in Arabidopsis Shoot Trichomes and Cotton Fiber

Journal of Integrative Plant Biology, 52(2), 205–220.

By: L. Betancur n, B. Singh n, R. Rapp*, J. Wendel*, M. Marks*, A. Roberts*, C. Haigler n

MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / classification; Arabidopsis Proteins / genetics; Arabidopsis Proteins / physiology; Cell Wall / metabolism; Cotton Fiber; Gene Expression Regulation, Plant / genetics; Gene Expression Regulation, Plant / physiology; Glucosyltransferases / classification; Glucosyltransferases / genetics; Glucosyltransferases / physiology; Gossypium / genetics; Gossypium / metabolism; Oligonucleotide Array Sequence Analysis; Phylogeny; Plant Proteins / classification; Plant Proteins / genetics; Plant Proteins / physiology; Plant Shoots / genetics; Plant Shoots / metabolism
TL;DR: Cumulatively, the data show that the xylem of arabidopsis provides the best model for secondary wall cellulose synthesis in cotton fiber and CesA genes within a "cell wall toolbox" are used in diverse ways for the construction of particular specialized cell walls. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2010 chapter

Physiological and Anatomical Factors Determining Fiber Structure and Utility

In Physiology of Cotton (pp. 33–47).

By: C. Haigler n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Crossref, NC State University Libraries
Added: October 13, 2020

2010 journal article

Sequencing and Utilization of the Gossypium Genomes

Tropical Plant Biology, 3(2), 71–74.

By: A. Paterson*, J. Rong*, A. Gingle*, P. Chee*, E. Dennis*, D. Llewellyn*, L. Dure*, C. Haigler n ...

TL;DR: Progress toward a reference sequence for the smallest Gossypium genome is a logical stepping-stone toward revealing diversity in the remaining seven genomes that permitted GossYPium species to adapt to a wide range of ecosystems in warmer arid regions of the world. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2009 journal article

A Specialized Outer Layer of the Primary Cell Wall Joins Elongating Cotton Fibers into Tissue-Like Bundles

Plant Physiology, 150(2), 684–699.

By: B. Singh n, U. Avci n, S. Eichler Inwood, M. Grimson n, J. Landgraf n, D. Mohnen n, I. Sørensen n, C. Wilkerson n, W. Willats n, C. Haigler n

MeSH headings : Cell Wall / physiology; Cell Wall / ultrastructure; Cotton Fiber; Cryoelectron Microscopy; Gossypium / cytology; Gossypium / growth & development; Gossypium / ultrastructure; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Polysaccharides / metabolism; Time Factors
TL;DR: Data show that adhesion modulated by an outer layer of the primary wall can coordinate the extensive growth of a large group of cells and illustrate dynamic changes in primary wall structure and composition occurring during the differentiation of one cell type that spends only part of its life as a tissue. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber

Plant Cell Reports, 28(7), 1023–1032.

By: B. Singh n, H. Cheek n & C. Haigler n

author keywords: Auxin; Cellulose; Cotton ovule culture; Fiber differentiation; Primary cell wall; Secondary cell wall
MeSH headings : Cell Wall / metabolism; Cellulose / biosynthesis; Cotton Fiber; Gene Expression Profiling; Gene Expression Regulation, Plant; Gossypium / genetics; Gossypium / growth & development; Gossypium / metabolism; Indoleacetic Acids / metabolism; Naphthaleneacetic Acids / metabolism; Plant Growth Regulators / metabolism; Seeds / growth & development; Seeds / metabolism
TL;DR: It is demonstrated that secondary wall cellulose synthesis is under strong transcriptional control that is influenced by auxin and must be specifically characterized in the cotton ovule/fiber culture system given the many protocol variables employed in different laboratories. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2009 chapter

Biogenesis of Cellulose Nanofibrils by a Biological Nanomachine

In The Nanoscience and Technology of Renewable Biomaterials (pp. 43–59).

By: C. Haigler n & A. Roberts*

Sources: Crossref, NC State University Libraries
Added: October 17, 2020

2009 chapter

Genomics of cotton fiber secondary wall deposition and cellulose biogenesis

In Genetics and genomics of cotton (pp. 385–417).

By: C. Haigler n, B. Singh*, G. Wang & D. Zhang

TL;DR: This chapter provides an overview of cellulose biogenesis, summarizes how cotton fiber has previously facilitated unique insights in this field, and explains how cellulose is important in terms of cotton fiber physical properties. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2009 journal article

Plant cell calcium-rich environment enhances thermostability of recombinantly produced α-amylase from the hyperthermophilic bacterium Thermotoga maritime

Biotechnology and Bioengineering, 104(5), 947–956.

By: M. Santa-Maria n, C. Chou n, G. Yencho n, C. Haigler n, W. Thompson n, R. Kelly n, B. Sosinski n

author keywords: hyperthermophilic enzymes; starch processing; transgenic plants; tobacco NT1 Cell cultures; biofuel
MeSH headings : Calcium / pharmacology; Coenzymes / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Hot Temperature; Plants, Genetically Modified / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Tobacco / enzymology; Tobacco / genetics; alpha-Amylases / chemistry; alpha-Amylases / genetics; alpha-Amylases / metabolism
TL;DR: In a proof of concept study, tobacco cell cultures were used as model system to test in planta production of a hyperthermophilic α‐amylase from Thermotoga maritima, demonstrating the importance of a calcium‐rich environment for thermostability, as well as the advantage of producing this enzyme directly in plant cells where calcium is readily available. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

Rapid Microwave Processing of Winter Cereals for Histology Allows Identification of Separate Zones of Freezing Injury in the Crown

Crop Science, 49(5), 1837–1842.

By: D. Livingston n, T. Tuong n, C. Haigler n, U. Avci n & S. Tallury n

TL;DR: High quality sections from the crowns of oat, barley, wheat, and rye indicate that this procedure is valid for all winter cereals, and it is predicted that the protocol will be useful for other grasses as well. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2008 journal article

A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses

PLANT JOURNAL, 56(3), 483–492.

By: M. Marks*, L. Betancur n, E. Gilding*, F. Chen*, S. Bauer*, J. Wenger*, R. Dixon*, C. Haigler n

author keywords: trichome isolation; trichome cell wall; trichome gene expression; lignin; pectin
MeSH headings : Arabidopsis / chemistry; Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis Proteins / isolation & purification; Cell Separation / methods; Cell Wall / chemistry; DNA, Plant / genetics; DNA, Plant / isolation & purification; Gene Expression Profiling; Genes, Plant; Genome, Plant; Lignin / chemistry; Microscopy, Electron, Transmission; Monosaccharides / chemistry; Plant Epidermis / cytology; Plant Epidermis / genetics; Plant Leaves / cytology; Plant Leaves / genetics; Polymerase Chain Reaction; RNA, Plant / genetics; RNA, Plant / isolation & purification
TL;DR: The monosaccharide analysis and positive staining with ruthenium red indicates that the walls also contain a large portion of pectin, which suggests that the polysaccharides of the trichome cell walls are more like those of typical primary walls even though the wall becomes quite thick. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Cysteine proteases XCP1 and XCP2 aid micro-autolysis within the intact central vacuole during xylogenesis in Arabidopsis roots

PLANT JOURNAL, 56(2), 303–315.

By: U. Avci n, H. Petzold*, I. Ismail*, E. Beers* & C. Haigler n

author keywords: programmed cell death; tracheary element autolysis; cysteine protease; vacuole; microwave-assisted fixation; immunolabeling
MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / ultrastructure; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Cysteine Endopeptidases / genetics; Cysteine Endopeptidases / metabolism; DNA, Bacterial / genetics; DNA, Plant / genetics; Genes, Plant; Genetic Complementation Test; Genotype; Immunoblotting; Microscopy, Electron, Transmission; Mutagenesis, Insertional; Plant Roots / genetics; Plant Roots / metabolism; Plant Roots / ultrastructure; Vacuoles / genetics; Vacuoles / metabolism; Vacuoles / ultrastructure; Xylem / genetics; Xylem / metabolism; Xylem / ultrastructure
TL;DR: These data demonstrate that two paralogous Arabidopsis thaliana proteases, XYLEM CYSTEINE PROTEASE1 (XCP1) and XCP2, participated in micro-autolysis within the intact central vacuole before mega-autolynsis was initiated by tonoplast implosion. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Geminivirus-Mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Cotton

Plant Physiology, 148(1), 41–50.

By: J. Tuttle n, A. Idris n, J. Brown n, C. Haigler n & D. Robertson n

MeSH headings : Animals; Capsid Proteins / physiology; Cold Temperature; DNA, Viral / analysis; Flowers / chemistry; Geminiviridae / physiology; Gene Silencing; Genetic Markers; Genetic Vectors / analysis; Genetic Vectors / physiology; Gossypium / chemistry; Gossypium / virology; Host-Pathogen Interactions; Insect Vectors / physiology; Plant Diseases / virology; Plant Leaves / chemistry; Plant Roots / chemistry
TL;DR: The experiments establish the use of the CLCrV silencing vector to study gene function in cotton and show that temperature can have a major impact on the extent of geminivirus-induced gene silencing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2008 journal article

XND1, a member of the NAC domain family in Arabidopsis thaliana, negatively regulates lignocellulose synthesis and programmed cell death in xylem

PLANT JOURNAL, 53(3), 425–436.

By: C. Zhao*, U. Avci n, E. Grant*, C. Haigler n & E. Beers*

author keywords: Arabidopsis; xylem; tracheary element; cell wall; programmed cell death; NAC domain
MeSH headings : Apoptosis / genetics; Apoptosis / physiology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis / ultrastructure; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Cell Wall / metabolism; Cellulose / biosynthesis; Gene Expression Regulation, Plant / drug effects; Leupeptins / pharmacology; Lignin / biosynthesis; Microscopy, Electron, Transmission; Mutagenesis, Insertional; Plants, Genetically Modified; Reverse Transcriptase Polymerase Chain Reaction; Xylem / genetics; Xylem / metabolism; Xylem / ultrastructure
TL;DR: Findings suggest that XND1 affects tracheary element growth through regulation of secondary wall synthesis and programmed cell death in Arabidopsis thaliana. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 conference paper

New controls of cotton fiber development and quality illuminated through integration of genomic, cell biological, and biochemical analyses

Proceedings of the World Cotton Research Conference-4. Presented at the World Cotton Research Conference, Lubbock, TX.

By: B. Singh, U. Avci, M. Grimson, S. Inwood, J. Landgraf, D. Mohnen, I. Sorensen, C. Wilkerson, W. Willats, C. Haigler

Event: World Cotton Research Conference at Lubbock, TX on September 10-14, 2007

Source: NC State University Libraries
Added: April 24, 2021

2007 chapter

Substrate supply for cellulose synthesis and its stress sensitivity in the cotton fiber

In R. M. Brown & I. Saxena (Eds.), Cellulose: Molecular and structural biology (pp. 145–166).

By: C. Haigler n

Ed(s): . R. M. Brown & I. Saxena

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2007 journal article

Toward Sequencing Cotton (Gossypium) Genomes: Figure 1.

Plant Physiology, 145(4), 1303–1310.

By: Z. Chen*, B. Scheffler*, E. Dennis*, B. Triplett*, T. Zhang*, W. Guo*, X. Chen*, D. Stelly* ...

MeSH headings : Genome, Plant; Gossypium / genetics; Sequence Analysis, DNA
TL;DR: Despite rapidly decreasing costs and innovative technologies, sequencing of angiosperm genomes is not yet undertaken lightly and the difficulties of sequencing and assembling complex genomes de novo are not yet addressed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Crossref, NC State University Libraries
Added: March 21, 2021

2007 journal article

Transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions

PLANT MOLECULAR BIOLOGY, 63(6), 815–832.

By: C. Haigler n, B. Singh n, D. Zhang*, S. Hwang, C. Wu*, W. Cai*, M. Hozain*, W. Kang* ...

author keywords: carbon partitioning; cotton fiber quality; environmental stress; sucrose, starch and cellulose metabolism; transgenic cotton
MeSH headings : Blotting, Western; Carbon Dioxide / pharmacology; Carbon Radioisotopes; Cotton Fiber; Gene Expression Regulation, Plant / drug effects; Gene Expression Regulation, Plant / radiation effects; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Gossypium / genetics; Gossypium / growth & development; Gossypium / metabolism; Light; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / metabolism; Plants, Genetically Modified; Reverse Transcriptase Polymerase Chain Reaction; Spinacia oleracea / enzymology; Spinacia oleracea / genetics; Starch / metabolism; Sucrose / metabolism; Temperature
TL;DR: A family of transgenic cotton plants was produced that over-expressed spinach sucrose-phosphate synthase (SPS) because of its role in regulation of sucrose synthesis in photosynthetic and heterotrophic tissues and had higher fiber micronaire and maturity ratio associated with greater thickness of the cellulosic secondary wall. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

A global assembly of cotton ESTs

GENOME RESEARCH, 16(3), 441–450.

By: J. Udall*, J. Swanson*, K. Haller*, R. Rapp*, M. Sparks*, J. Hatfield*, Y. Yu*, Y. Wu* ...

MeSH headings : DNA, Complementary / genetics; Diploidy; Expressed Sequence Tags; Gene Expression Profiling / methods; Genome, Plant; Gossypium / genetics; Molecular Sequence Data; Polyploidy; Sequence Analysis, DNA
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 conference paper

Analysis of cell wall synthesis in feeding cells formed by root-knot nematodes

Biology of plant-microbe interactions - Proceedings of the 12th International Congress on Molecular Plant-Microbe Interactions, Mérida, Yucatán, México, 281–285.

By: L. Hudson, C. Haigler & E. Davis

Event: 12th International Congress on Molecular Plant-Microbe Interactions, at Mérida, Yucatán, México on December 14-19, 2005

Source: NC State University Libraries
Added: April 24, 2021

2006 patent

Chitinase encoding DNA molecules from cotton expressed preferentially in secondary walled cells during secondary wall deposition and a corresponding promoter

Washington, DC: U.S. Patent and Trademark Office.

By: C. Haigler, H. Zhang, C. Wu & D. Zhang

Source: NC State University Libraries
Added: August 6, 2018

2006 chapter

Establishing the cellular and biophysical context of cellulose synthesis

In The science and lore of the plant cell wall: Biosynthesis, structure and function (pp. 97–105). Boca Raton, FL: BrownWalker Press.

By: C. Haigler

Source: NC State University Libraries
Added: August 6, 2018

2006 patent

Transgenic fiber producing plants with increased expression of sucrose phosphate synthase

Washington, DC: U.S. Patent and Trademark Office.

By: C. Haigler & A. Holaday

Source: NC State University Libraries
Added: August 6, 2018

2005 review

Biotechnological improvement of cotton fibre maturity

[Review of ]. PHYSIOLOGIA PLANTARUM, 124(3), 285–294.

By: C. Haigler n, D. Zhang n & C. Wilkerson*

TL;DR: The prospects and tools for controlling cotton fibre secondary wall thickness and key genes that participate in the regulation and control of secondary wall cellulose biogenesis must be identified are focused on. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Long-term night chilling of cotton (Gossypium hirsutum) does not result in reduced CO2 assimilation

FUNCTIONAL PLANT BIOLOGY, 32(7), 655–666.

By: B. Singh n, L. Haley*, J. Nightengale*, W. Kang*, C. Haigler n & A. Holaday*

author keywords: carbohydrates; cotton; night chilling; photosynthesis; sucrose-phosphate synthase
TL;DR: Despite the elevated leaf carbohydrate content when plants were grown to maturity with night chilling, no reduction in A, gs, carboxylation capacity, electron transport capacity, triose-phosphate utilisation capacity occurred, and growth in cool nights tended to retard the diminishing of photosynthetic parameters and gs for aging stem and subtending leaves. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 book

Nanotechnology for the forest products industry: Vision and technology roadmap

By: R. Atalla*, J. Beecher*, B. Caron, J. Catchmark*, Y. Deng*, W. Glasser*, D. Gray*, C. Haigler n ...

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2004 journal article

Cool temperature hinders flux from glucose to sucrose during cellulose synthesis in secondary wall stage cotton fibers

CELLULOSE, 11(3-4), 339–349.

By: L. Martin* & C. Haigler n

author keywords: cellulose synthesis; cool temperature stress; cotton fiber; fructose; glucose; Gossypium hirsutum; in vitro culture; metabolic flux; secondary wall; sucrose synthesis
TL;DR: Data are presented that implicate sucrose synthesis within cotton fibers as a particularly cool temperature-sensitive step in the partitioning of carbon to cellulose. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls

PLANT MOLECULAR BIOLOGY, 54(3), 353–372.

By: D. Zhang n, M. Hrmova*, C. Wan*, C. Wu*, J. Balzen*, W. Cai*, J. Wang*, L. Densmore* ...

author keywords: cellulose; chitin binding; cotton fiber; molecular modeling; promoter activity; secondary cell wall
MeSH headings : Amino Acid Sequence; Blotting, Northern; Cell Wall / genetics; Cell Wall / metabolism; Chitinases / chemistry; Chitinases / genetics; Chitinases / metabolism; DNA, Complementary / chemistry; DNA, Complementary / genetics; DNA, Plant / chemistry; DNA, Plant / genetics; Gene Expression Profiling; Gene Expression Regulation, Plant; Glucuronidase / genetics; Glucuronidase / metabolism; Gossypium / cytology; Gossypium / genetics; Models, Molecular; Molecular Conformation; Molecular Sequence Data; Multigene Family / genetics; Phylogeny; Plant Proteins / chemistry; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; RNA, Plant / genetics; RNA, Plant / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid
TL;DR: Evidence from other researchers that mutants in an Arabidopsis gene within the GhCTL group are cellulose-deficient with phenotypes indicative of altered primary cell walls, suggest that members of the Gh CTL group of chitinase-like proteins are essential for cellulose synthesis in primary and secondary cell walls. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Roles of microtubules and cellulose microfibril assembly in the localization of secondary-cell-wall deposition in developing tracheary elements

PROTOPLASMA, 224(3-4), 217–229.

By: A. Roberts*, A. Frost*, E. Roberts* & C. Haigler*

author keywords: amiprophosmethyl; cellulose microfibril assembly; Congo Red; cortical microtubule; Evans Blue; xylogenic suspension culture
MeSH headings : Asteraceae; Cell Wall / drug effects; Cell Wall / physiology; Cell Wall / ultrastructure; Cells, Cultured; Cellulose / metabolism; Congo Red / pharmacology; Evans Blue / pharmacology; Fluorescent Antibody Technique; Microfibrils / drug effects; Microfibrils / physiology; Microfibrils / ultrastructure; Microtubules / drug effects; Microtubules / physiology; Microtubules / ultrastructure; Models, Biological; Plant Cells; Plants / metabolism
TL;DR: Examination of non-dye-treated cultures following application of microtubule inhibitors during various stages of secondary-cell-wall deposition revealed that the pattern became fixed at an early stage such that deposition remained localized in the absence of cortical microtubules. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Characterization of a novel cellulose synthesis inhibitor

PLANTA, 217(6), 922–930.

By: B. Kiedaisch*, R. Blanton n & C. Haigler n

author keywords: cellulose synthesis inhibitor; freeze fracture; rosettes; Sorghum; Zinnia
MeSH headings : Asteraceae / cytology; Asteraceae / drug effects; Asteraceae / physiology; Asteraceae / ultrastructure; Benzamides / pharmacology; Cellulose / antagonists & inhibitors; Cellulose / biosynthesis; Freeze Fracturing; Sorghum; Thiazines / pharmacology; Triazines / pharmacology
TL;DR: Freeze-fracture electron microscopy showed that the plasma membrane below the patterned thickenings of AE F150944-treated tracheary elements was depleted of cellulose-synthase-containing rosettes, which appeared to be inserted intact into the plasma membranes followed by their rapid disaggregation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Localization of sucrose synthase and callose in freeze-substituted secondary-wall-stage cotton fibers

PROTOPLASMA, 221(3-4), 175–184.

By: . Salnikov*, M. Grimson*, R. Seagull* & C. Haigler*

author keywords: cell wall; cellulose synthesis; cryogenic specimen preparation; Gossypium hirsutum; immunolabeling; transmission electron microscopy
MeSH headings : Cell Wall / chemistry; Cell Wall / ultrastructure; Cellulose / biosynthesis; Cotton Fiber; Cryopreservation; Glucans / analysis; Glucosyltransferases / analysis; Gossypium / chemistry; Gossypium / enzymology; Immunohistochemistry; Microscopy, Electron; Polymers / analysis
TL;DR: Methods for cryogenic fixation, freeze substitution, and embedding were developed to preserve the cellular structure and protein localization of secondary-wall-stage cotton fibers accurately for the first time, revealing native ultrastructure, including numerous active Golgi bodies, multivesicular bodies, and proplastids. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 conference paper

Progress and emerging questions in understanding cellulose biogenesis

Proceedings, Vol. 1: 12th International Symposium on Wood and Pulping Chemistry, 9–16. Madison: University of Wisconsin.

By: C. Haigler

Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

The regulation of metabolic flux to cellulose, a major sink for carbon in plants

METABOLIC ENGINEERING, 4(1), 22–28.

By: D. Delmer & C. Haigler*

MeSH headings : Carbon / metabolism; Cellulose / biosynthesis; Cellulose / metabolism; Gene Expression Regulation, Plant; Genes, Plant; Models, Genetic; Mutation; Plants / genetics; Plants / metabolism; Protein Engineering; Starch / metabolism
TL;DR: This review outlines basic knowledge about the genes and proteins that are involved in cellulose biosynthesis and presents a model that summarizes the current thinking on the overall cellulOSE biosynthesis pathway, strategies that might be used for altering the flux of carbon into this pathway. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 patent

Transgenic cotton plants with altered fiber characteristics transformed with a sucrose phosphate synthase nucleic acid

Washington, DC: U.S. Patent and Trademark Office.

By: C. Haigler & A. Holaday

Source: NC State University Libraries
Added: August 6, 2018

2001 review

Carbon partitioning to cellulose synthesis

[Review of ]. PLANT MOLECULAR BIOLOGY, 47(1-2), 29–51.

By: C. Haigler*, M. Ivanova-Datcheva*, P. Hogan*, . Salnikov*, S. Hwang*, K. Martin*, D. Delmer*

author keywords: calcium; carbon partitioning; cellulose; cotton fiber; sucrose synthase; phosphorylation
TL;DR: A model for metabolism related to cellulose synthesis is developed that implicates the changing intracellular localization of sucrose synthase as a molecular switch between survival metabolism and growth and/or differentiation processes involving cellulose synthase. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 article

Regulation of cellulose biosynthesis in developing xylem

MOLECULAR BREEDING OF WOODY PLANTS, PROCEEDINGS, Vol. 18, pp. 1–9.

By: C. Haigler*, V. Babb*, S. Hwang* & . Salnikov*

author keywords: cellulose synthesis; cellulose synthase; primary wall; secondary wall; sucrose phosphate synthase; sucrose synthase; tracheary element; Zinnia elegans
TL;DR: The advantages of using isolated mesophyll cells of Zinnia elegans as a model to study the regulation of cellulose synthesis will be discussed and a composite cellular and metabolic model for cellulose synthesisation will be presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Sucrose phosphate synthase activity rises in correlation with high-rate cellulose synthesis in three heterotrophic systems

Plant Physiology, 127, 1234–1242.

By: V. Babb* & C. Haigler*

TL;DR: The capacity of cellulose sink cells to synthesize Suc was tested by analyzing the Suc phosphate synthase (SPS) activity of three heterotrophic systems with cellulose-rich secondary walls, and SPS activity rose in all three systems during periods of maximum cellulose deposition within secondary walls. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2001 article

Sucrose synthase localizes to cellulose synthesis sites in tracheary elements

Salnikov, VV, Grimson, M. J., Delmar, D. P., & Haigler, C. H. (2001, July). PHYTOCHEMISTRY, Vol. 57, pp. 823–833.

By: . Salnikov*, M. Grimson*, D. Delmar & C. Haigler*

author keywords: Zinnia elegans; compositae; electron microscopic immunolocalization; freeze substitution; cellulose synthesis; sucrose synthase; actin; microtubule; tracheary element; secondary cell wall
MeSH headings : Actins / analysis; Actins / metabolism; Asteraceae / cytology; Asteraceae / metabolism; Asteraceae / ultrastructure; Cell Differentiation; Cells, Cultured; Cellulose / biosynthesis; Glucosyltransferases / analysis; Microscopy, Electron; Microscopy, Immunoelectron; Microtubules / metabolism; Microtubules / ultrastructure; Models, Biological
TL;DR: The results show that the preferential localization of sucrose synthase at sites of high-rate cellulose synthesis can be generalized beyond cotton fibers, and they establish a spatial context for further work on a multi-protein complex that may facilitate secondary wall cellulOSE synthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 chapter

Optimizing secondary wall synthesis in cotton fibers

In Genetic control of cotton fiber and seed quality (pp. 147–165). Cary, NC: Cotton Incorporated.

By: C. Haigler, W. Cai, J. Gannaway, M. Grimson, E. Hequet, A. Holaday, J. Huang, T. Jaradat ...

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

A secreted factor induces cell expansion and formation of metaxylem-like tracheary elements in xylogenic suspension cultures of Zinnia

PLANT PHYSIOLOGY, 115(2), 683–692.

By: A. Roberts*, S. Donovan* & C. Haigler*

TL;DR: It is suggested that the secreted factor may be an oligosaccharide involved in the coordination of cell expansion and differentiation and the regulation of the protoxylem-like to metaxylem -like transition in xylogenic suspension cultures. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1996 chapter

Cellulose biosynthesis

In J. P. K. M. Smallwood & D. J. Bowles (Eds.), Membranes: Specialized functions in plants (pp. 57–75). Oxford, UK: BIOS Scientific Publishers.

By: R. Blanton & C. Haigler

Ed(s): J. M. Smallwood & D. Bowles

Source: NC State University Libraries
Added: August 6, 2018

1996 journal article

Cellulose microfibrils, cell motility, and plasma membrane organization change in parallel during culmination in Dictyostelium discoideum

Journal of Cell Science, 109, 3079–3087.

By: M. Grimson, C. Haigler & R. Blanton

Source: NC State University Libraries
Added: August 6, 2018

1996 journal article

Detection of cellulose with improved specificity using laser-based instruments

BIOTECHNIC & HISTOCHEMISTRY, 71(5), 215–223.

By: J. Taylor*, C. Haigler*, D. Kilburn* & R. Blanton*

author keywords: cellulose; cellulose binding domain; laser scanning confocal microscopy; double staining; fluorescence
MeSH headings : Animals; Cellulase / metabolism; Cellulose / analysis; Chlorophyll; Dictyostelium / metabolism; Flow Cytometry / instrumentation; Fluorescent Antibody Technique; Lasers; Microscopy, Confocal / instrumentation; Microtubules / metabolism; Plants / metabolism; Sensitivity and Specificity
TL;DR: It is demonstrated that a cellulase and an isolated bacterial cellulose binding domain (CBD) conjugated to fluorescent dyes can be used for laser detection of cellulose with improved specificity and the presence or absence of cellulOSE can now be analyzed using common lasers. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1996 article

New hope for old dreams: Evidence that plant cellulose synthase genes have finally been identified

Haigler, C. H., & Blanton, R. L. (1996, October 29). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 93, pp. 12082–12085.

By: C. Haigler* & R. Blanton*

MeSH headings : Cellulose / biosynthesis; Conserved Sequence; DNA, Complementary / chemistry; Gossypium / genetics; Gram-Negative Bacteria / enzymology; Plants / enzymology
TL;DR: This finding is noteworthy because it provides the first likely direct route to unraveling the cellular and molecular details of plant cellulose biogenesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1995 journal article

A MEMBRANE-ASSOCIATED FORM OF SUCROSE SYNTHASE AND ITS POTENTIAL ROLE IN SYNTHESIS OF CELLULOSE AND CALLOSE IN PLANTS

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 92(20), 9353–9357.

By: Y. Amor*, C. Haigler*, S. Johnson*, M. Wainscott* & D. Delmer*

MeSH headings : Amino Acid Sequence; Cell Membrane / enzymology; Cellulose / metabolism; Electrophoresis, Polyacrylamide Gel; Glucans / biosynthesis; Glucosyltransferases / chemistry; Glucosyltransferases / isolation & purification; Glucosyltransferases / metabolism; Gossypium / enzymology; Immunohistochemistry; Kinetics; Molecular Sequence Data; Sequence Homology, Amino Acid; Sucrose / metabolism; Uridine Diphosphate Glucose / metabolism
TL;DR: Results support a model in which SuSy exists in a complex with the beta-glucan synthases and serves to channel carbon from sucrose to glucan, and the traditional observation that when UDPglucose is used as substrate in vitro, callose is the major product synthesized. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1994 journal article

CELL EXPANSION AND TRACHEARY ELEMENT DIFFERENTIATION ARE REGULATED BY EXTRACELLULAR PH IN MESOPHYLL CULTURES OF ZINNIA-ELEGANS L

PLANT PHYSIOLOGY, 105(2), 699–706.

By: A. Roberts* & C. Haigler*

TL;DR: The correlation between cellansion and delayed differentiation of large, metaxylem-like TEs may indicate a link between the regulatory mechanisms controlling cell expansion and TE differentiation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1994 article

FROM SIGNAL-TRANSDUCTION TO BIOPHYSICS - TRACHEARY ELEMENT DIFFERENTIATION AS A MODEL SYSTEM

HAIGLER, C. H. (1994, May). INTERNATIONAL JOURNAL OF PLANT SCIENCES, Vol. 155, pp. 248–250.

By: C. Haigler*

TL;DR: The three review articles that follow deal with the processes by which cells become competent for, then committed to, a terminal differentiation pathway culminating in cell death, which is essential for plant function, environmental adaptation, and the economic uses of wood. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1994 conference paper

Temperature dependence of fiber cellulose biosynthesis: Impact on fiber maturity and strength

Proceedings of the Biochemistry of Cotton Workshop, Galveston, TX, Sept. 28-30, 95–100. Raleigh, NC: Cotton Incorporated.

By: C. Haigler, J. Taylor & L. Martin

Source: NC State University Libraries
Added: August 6, 2018

1993 journal article

Cool temperature effects on cotton fiber initiation and elongation clarified using in vitro cultures

Crop Science, 33, 1258–1264.

By: W. Xie*, N. Trolinder* & C. Haigler*

TL;DR: Cotton ovules (Gossypium hirsutum L.) cultured in vitro were used as a manipulable and reproducible experimental system based on previous evidence that they provide a valid model for cotton fiber development. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1993 journal article

Patterned secondary wall assembly in tracheary elements occurs in a self-perpetuating cascade

Acta Botanica Neerlandica, 42, 153–163.

By: J. Taylor* & C. Haigler*

TL;DR: The behaviours are presented of newly-synthesized xylan and putative glycine-rich protein during patterned secondary cell-wall biogenesis in drug-treated tracheary elements (TEs) differentiating in culture from isolated mesophyll cells of Zinnia elegans. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1992 journal article

A SIMPLIFIED MEDIUM FOR INVITRO TRACHEARY ELEMENT DIFFERENTIATION IN MESOPHYLL SUSPENSION-CULTURES FROM ZINNIA-ELEGANS L

PLANT CELL TISSUE AND ORGAN CULTURE, 28(1), 27–35.

By: A. Roberts*, L. Koonce* & C. Haigler*

author keywords: CALCIUM; PLANT CELL SUSPENSION CULTURE; TRACHEARY ELEMENT DIFFERENTIATION; XYLOGENESIS; ZINNIA-ELEGANS L
TL;DR: This simplified medium is particularly suitable for studies of the role of Ca2+ in tracheary element differentiation due to the elimination of myo-inositol, an intermediate in the phosphatidyl inositol signal transduction pathway and reduction in the concentrations of Mg2+ and Mn2+, which block calcium channels. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

DISPERSED LIGNIN IN TRACHEARY ELEMENTS TREATED WITH CELLULOSE SYNTHESIS INHIBITORS PROVIDES EVIDENCE THAT MOLECULES OF THE SECONDARY CELL-WALL MEDIATE WALL PATTERNING

PLANT JOURNAL, 2(6), 959–970.

By: J. Taylor*, T. Owen*, L. Koonce* & C. Haigler*

TL;DR: The data suggest that normal patterned wall assembly in TEs occurs in a self-perpetuating cascade in which some molecules of the secondary cell wall mediate patterning of others, and the localization of lignin is ultimately dependent on the localized cellulose. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

EFFECTS OF CYCLING TEMPERATURES ON FIBER METABOLISM IN CULTURED COTTON OVULES

PLANT PHYSIOLOGY, 100(2), 979–986.

By: E. Roberts*, N. Rao, J. Huang, N. Trolinder & C. Haigler*

TL;DR: Data indicate that cellulose synthesis and respiration respond differently to cool temperatures, which is consistent with previous observations on the responses of fiber elongation and weight gain to cycling temperatures in vitro and in the field. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

Methylxanthines reversibly inhibit tracheary element differentiation in suspension cultures of Zinnia elegans

Planta (Online), 186, 586–592.

By: A. Roberts* & C. Haigler

TL;DR: Tracheary-element (TE) differentiation in suspension-cultured mesophyll cells of Zinnia elegans L. was completely inhibited by caffeine and theophylline only when these methylxanthines were applied at least 8 h prior to the appearance of secondary cell-wall thickenings, indicating the involvement of amethylxanthine-inhibitable event in TE differentiation that is distinguishable from an event dependent on influx of extracellular calcium. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1992 conference paper

The crystallinity of cotton cellulose in relation to cotton improvement

Proceedings, Cotton Fiber Cellulose: Structure, Function, and Utilization Conference, 211–225. Memphis, TN: National Cotton Council of America.

By: C. Haigler

Source: NC State University Libraries
Added: August 6, 2018

1991 journal article

Buffer capacity of cotton cells and effects of extracellular pH on growth and somatic embryogenesis in cotton cell suspensions

In Vitro Cellular & Developmental Biology. Plant, 27P, 147–152.

By: X. Shang*, J. Huang*, C. Haigler* & N. Trolinder*

TL;DR: The metabolic activity of living cells accounts for the adjustment and stabilization of pH during the log phase of growth and the starting pH of the culture medium affects longterm viability, growth, and differentiation of the cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1991 journal article

CULTURED OVULES AS MODELS FOR COTTON FIBER DEVELOPMENT UNDER LOW-TEMPERATURES

PLANT PHYSIOLOGY, 95(1), 88–96.

By: C. Haigler*, N. Rao*, E. Roberts*, J. Huang*, D. Upchurch* & N. Trolinder

TL;DR: These results demonstrate that cool temperature effects on fiber development are at least partly fiber/ovule-specific events; they do not depend on whole-plant physiology; and cultured ovules are valid models for research on the regulation of the field cool temperature response. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1991 chapter

The relationship between polymerization and crystallization in cellulose biogenesis

In C. H. Haigler & P. Weimer (Eds.), Biosynthesis and biodegradation of cellulose (pp. 99–124). New York: Marcel Dekker.

By: C. Haigler

Ed(s): C. Haigler & P. Weimer

Source: NC State University Libraries
Added: August 6, 2018

1990 chapter

Relationship between polymerization and crystallization in microfibril biogenesis

In C. H. Haigler & P. Weimer (Eds.), Biosynthesis and Biodegradation of Cellulose (pp. 99–124). New York: Marcel Dekker.

By: C. Haigler

Ed(s): C. Haigler & P. Weimer

Source: NC State University Libraries
Added: April 24, 2021

1990 journal article

TRACHEARY-ELEMENT DIFFERENTIATION IN SUSPENSION-CULTURED CELLS OF ZINNIA REQUIRES UPTAKE OF EXTRACELLULAR CA-2+ - EXPERIMENTS WITH CALCIUM-CHANNEL BLOCKERS AND CALMODULIN INHIBITORS

PLANTA, 180(4), 502–509.

By: A. Roberts* & C. Haigler*

TL;DR: Results indicate that TE differentiation involves at least two calcium-regulated events: one calmodulin-dependent and occurring shortly after exposure to inductive conditions, and the other cal modulin-independent and occurring just prior to secondary cell-wall deposition. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1989 chapter

Electron diffraction analysis of altered cellulose: Implications for mechanisms of biogenesis

In Cellulose and wood: Chemistry and technology (pp. 493–506). New York: John Wiley.

By: C. Haigler & H. Chanzy

Source: NC State University Libraries
Added: August 6, 2018

1989 journal article

Rise in chlorotetracycline fluorescence accompanies tracheary element differentiation in suspension cultures of Zinnia

Protoplasma, 152, 37–45.

By: A. Roberts* & C. Haigler*

TL;DR: Developing tracheary elements in suspension cultures of Zinnia elegans fluoresce intensely relative to non-differentiating cells when stained with chlorotetracycline (CTC), a fluorescent chelate probe for membrane associated calcium, suggesting that a change in calcium uptake or subcellular distribution accompanies the onset oftracheary element differentiation. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1989 conference paper

Structural aspects of tracheary element differentiation in suspension cultures of Zinnia elegans

Proceedings of the 47th Annual Meeting of the Electron Microscopy Society of America, 768–769. San Francisco, CA: San Francisco Press.

By: C. Haigler & A. Roberts

Source: NC State University Libraries
Added: August 6, 2018

1988 journal article

ELECTRON-DIFFRACTION ANALYSIS OF THE ALTERED CELLULOSE SYNTHESIZED BY ACETOBACTER-XYLINUM IN THE PRESENCE OF FLUORESCENT BRIGHTENING AGENTS AND DIRECT DYES

JOURNAL OF ULTRASTRUCTURE AND MOLECULAR STRUCTURE RESEARCH, 98(3), 299–311.

By: C. Haigler* & H. Chanzy

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1986 journal article

ABSCISIC-ACID CONTROL OF LECTIN ACCUMULATION IN WHEAT SEEDLINGS AND CALLUS-CULTURES - EFFECTS OF EXOGENOUS ABA AND FLURIDONE

PLANT PHYSIOLOGY, 80(1), 167–171.

By: N. Raikhel*, B. Palevitz* & C. Haigler*

TL;DR: 10 micromolar abscisic acid produces an average two to three-fold enhancement in the amount of lectin in the shoot base and the terminal portion of the root system of hydroponically grown wheat seedlings, indicating that lectin synthesis is under ABA control in both wheat embryos and adult plants. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1986 journal article

TRANSPORT OF ROSETTES FROM THE GOLGI-APPARATUS TO THE PLASMA-MEMBRANE IN ISOLATED MESOPHYLL-CELLS OF ZINNIA-ELEGANS DURING DIFFERENTIATION TO TRACHEARY ELEMENTS IN SUSPENSION-CULTURE

PROTOPLASMA, 134(2-3), 111–120.

By: C. Haigler* & R. Brown*

TL;DR: The data presented indicate that the Golgi apparatus has a critical role in the control of cell wall deposition because it is involved not only in the synthesis and export of matrix components but also in the export of an important component of the cellulose synthesizing apparatus. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1985 chapter

The functions and biogenesis of native cellulose

In Cellulose chemistry and its applications (pp. 30–83). Chichester, Eng.: Ellis Horwood.

By: C. Haigler

Source: NC State University Libraries
Added: August 6, 2018

1983 chapter

The biosynthesis and degradation of cellulose. (Journal of applied polymer science. Applied polymer symposium; 37)

In Proceedings of the Ninth Cellulose Conference: held at Syracuse, New York, May 24-27, 1982 (pp. 33–78). New York: Wiley.

By: R. Brown, C. Haigler, J. Suttie, A. White, E. Roberts, C. Smith, T. Itoh, C. Cooper

Source: NC State University Libraries
Added: August 6, 2018

1982 journal article

ALTERATION OF CELLULOSE MICROFIBRIL FORMATION IN EUKARYOTIC CELLS - CALCOFLUOR WHITE INTERFERES WITH MICROFIBRIL ASSEMBLY AND ORIENTATION IN OOCYSTIS-APICULATA

PROTOPLASMA, 113(1), 1–9.

By: E. Roberts*, R. Seagull*, C. Haigler* & R. Brown*

TL;DR: It is demonstrated that Calcofluor also disrupts cell wall assembly in the eukaryotic algaOocystis apiculata, and cells with severely disrupted walls are unable to complete their normal life cycle. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1982 journal article

ALTERATION OF INVIVO CELLULOSE RIBBON ASSEMBLY BY CARBOXYMETHYLCELLULOSE AND OTHER CELLULOSE DERIVATIVES

JOURNAL OF CELL BIOLOGY, 94(1), 64–69.

By: C. Haigler*, A. White, R. Brown & K. Cooper

MeSH headings : Acetobacter / metabolism; Acetobacter / ultrastructure; Carboxymethylcellulose Sodium / pharmacology; Cell Membrane / metabolism; Cellulose / analogs & derivatives; Cellulose / biosynthesis; Cytoskeleton / metabolism; Macromolecular Substances; Methylcellulose / analogs & derivatives
TL;DR: The data support the previous hypothesis that the cellulose ribbon of A. xylinum is formed by a hierarchical, cell- directed, self-assembly process and the regulation of cellulose microfibril size and wall extensibility in plant cell walls is discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1982 chapter

Biogenesis of cellulose I microfibrils occurs by cell-directed self-assembly in Acetobacter xylinum

In Cellulose and other natural polymer systems (pp. 273–296).

By: C. Haigler* & M. Benziman*

TL;DR: It is probable that there is a common biosynthetic mechanism among all organisms that produce crystalline cellulose I microfibrils, and any proposed mechanism of cellulose biogenesis must be consistent with the ultimate physical properties of the macromolecule. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1982 journal article

EXPERIMENTAL INDUCTION OF ALTERED NON-MICROFIBRILLAR CELLULOSE

SCIENCE, 218(4577), 1141–1142.

By: R. Brown*, C. Haigler* & K. Cooper*

TL;DR: Cellulose produced by Acetobacter xylinum was experimentally modified during its biosynthesis in the presence of fluorescent brightening agents, and sheets of cellulose were synthesized that could be converted to fibrils by washing with distilled water. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1981 conference paper

Probing the relationship of polymerization and crystallization in the biogenesis of cellulose

The Ekman-Days 1981: International Symposium on Wood and Pulping Chemistry, Stockholm, June 9-12, 1981. Stockholm: Swedish Paper Chemistry Institute.

By: C. Haigler & R. Brown

Source: NC State University Libraries
Added: August 6, 2018

1980 journal article

CALCOFLUOR WHITE ST ALTERS THE INVIVO ASSEMBLY OF CELLULOSE MICROFIBRILS

SCIENCE, 210(4472), 903–906.

By: C. Haigler*, R. Malcolmbrown* & M. Benziman*

MeSH headings : Acetobacter / drug effects; Acetobacter / metabolism; Acetobacter / ultrastructure; Benzenesulfonates / pharmacology; Cellulose / biosynthesis; Glucans / metabolism; Stilbenes / pharmacology
TL;DR: Use of Calcofluor has made it possible to separate the processes of polymerization and crystallization leading to the biogenesis of cellulose microfibrils, and has suggested that crystallization occurs by a cell-directed process. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1980 journal article

CELLULOSE BIOGENESIS - POLYMERIZATION AND CRYSTALLIZATION ARE COUPLED PROCESSES IN ACETOBACTER-XYLINUM

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 77(11), 6678–6682.

By: M. Benziman*, C. Haigler*, R. Brown*, A. White* & K. Cooper*

TL;DR: Calcofluor White ST, stilbene derivative used commerically as an optical brightener for cellulose, increased the rate of glucose polymerization into cellulose by resting cells of the gram-negative bacterium Acetobacter xylinum, suggesting that coupling must be maintained for biogenesis of crystalline cellulose I. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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