@article{kreager_moon_mitchell_wu_peng_muller_huang_jiang_2023, title={Lung nodule biopsy guided using a 30 MHz ultrasound transducer: in vitro study}, volume={12488}, ISBN={["978-1-5106-6083-0"]}, ISSN={["1996-756X"]}, DOI={10.1117/12.2658585}, abstractNote={Ultrasound is often favored in biopsy guidance since it is non-ionizing, inexpensive, portable, and has a high frame rate. However, imaging probes that operate at a low frequency may not be able to differentiate between tiny targets and surrounding tissues clearly, and at a high frequency, it suffers from tissue scattering and signals attenuation, which is hard to image deeper targets such as lung tissues. In this study, we developed a biopsy needle (with a size of 18 G) integrated with a 30 MHz high-frequency ultrasound transducer (axial resolution: ~ 100 µm) for the lung nodule biopsy in vitro test. To mimic contrasting biological tissues, a melamine foam-gelatin phantom was developed. With an advancing step of 0.5 mm, the distance from the biopsy needle to the gelatin-foam boundary was estimated by the speed of sound in gelatin and the time-of-flight of the echo signal. The results showed that the 30 MHz ultrasound transducer can map the geometry of the gelatin-foam boundary, indicating the capability of distinguishing tumor and healthy lung tissue with this ultrasound-guided biopsy technique.}, journal={HEALTH MONITORING OF STRUCTURAL AND BIOLOGICAL SYSTEMS XVII}, author={Kreager, Ben and Moon, Sunho and Mitchell, Josh and Wu, Huaiyu and Peng, Chang and Muller, Marie and Huang, Chih-Chung and Jiang, Xiaoning}, year={2023} }
 @article{truong_chiu_lai_huang_jiang_huang_2022, title={Ca2+ signaling-mediated low-intensity pulsed ultrasound-induced proliferation and activation of motor neuron cells}, volume={124}, ISSN={["1874-9968"]}, DOI={10.1016/j.ultras.2022.106739}, abstractNote={Motor neuron diseases (MND) including amyotrophic lateral sclerosis and Parkinson disease are commonly neurodegenerative, causing a gradual loss of nerve cells and affecting the mechanisms underlying changes in calcium (Ca2+)-regulated dendritic growth. In this study, the NSC-34 cell line, a population of hybridomas generated using mouse spinal cord cells with neuroblastoma, was used to investigate the effect of low-intensity pulsed ultrasound (LIPUS) as part of an MND treatment model. After NSC-34 cells were seeded for 24 h, LIPUS stimulation was performed on the cells at days 1 and 3 using a non-focused transducer at 1.15 MHz for 8 min. NSC-34 cell proliferation and morphological changes were observed at various LIPUS intensities and different combinations of Ca2+ channel blockers. The nuclear translocation of Ca2+-dependent transcription factors was also examined. We observed that the neurite outgrowth and cell number of NSC-34 significantly increased with LIPUS stimulation at days 2 and 4, which may be associated with the treatment's positive effect on the activation of Ca2+-dependent transcription factors, such as nuclear factor of activated T cells and nuclear factor-kappa B. Our findings suggest that the LIPUS-induced Ca2+ signaling and transcription factor activation facilitate the morphological maturation and proliferation of NSC-34 cells, presenting a promising noninvasive method to improve stimulation therapy for MNDs in the future.}, journal={ULTRASONICS}, author={Truong, Thi-Thuyet and Chiu, Wen-Tai and Lai, Yi-Shyun and Huang, Hsien and Jiang, Xiaoning and Huang, Chih-Chung}, year={2022}, month={Aug} }