@inbook{da-silva_silva_inacio_araújo_modolo_2024, title={Current trends in H2S use in crops}, url={https://doi.org/10.1016/B978-0-323-99035-6.00009-9}, DOI={10.1016/B978-0-323-99035-6.00009-9}, abstractNote={Hydrogen sulfide (H2S) is a gasotransmitter produced by plant cells that contributes to plant growth, particularly when plants experience environmental stresses. Using H2S donors in agriculture is a strategy for improving crop production. This chapter presents the state of the art in H2S application to crops grown under uncontrolled environments, discussing case studies of some unconventional crops—plants with low commercial appeal that humans can consume as food without the plants becoming indispensable for feeding. The relationship between H2S and beneficial microorganisms in crop production is also reviewed. Considering the results obtained for crops grown at a small scale under uncontrolled conditions, we present some challenges and perspectives for using H2S in field crops.}, author={Da-Silva, Cristiane Jovelina and Silva, Thamara Ferreira and Inacio, Gabrielle Marques and Araújo, Lara Matos and Modolo, Luzia Valentina}, year={2024} }
 @inbook{ali_banerjee_bhardwaj_borokotoky_corpas_da-silva_silva maia_silva neto_araújo_fátima_et al._2024, title={List of contributors}, url={https://doi.org/10.1016/B978-0-323-99035-6.01002-2}, DOI={10.1016/B978-0-323-99035-6.01002-2}, booktitle={Elsevier}, author={Ali, Sajid and Banerjee, Aditya and Bhardwaj, Savita and Borokotoky, Subhomoi and Corpas, Francisco J. and Da-Silva, Cristiane Jovelina and Silva Maia, Angélica Faleiros and Silva Neto, Leonardo and Araújo, Lara Matos and Fátima, Ângelo and et al.}, year={2024} }
 @article{haverroth_da-silva_taggart_oliveira_cardoso_2024, title={Shoot hydraulic impairments induced by root waterlogging: Parallels and contrasts with drought}, volume={6}, ISSN={["1532-2548"]}, url={https://doi.org/10.1093/plphys/kiae336}, DOI={10.1093/plphys/kiae336}, abstractNote={Soil waterlogging and drought correspond to contrasting water extremes resulting in plant dehydration. Dehydration in response to waterlogging occurs due to impairments to root water transport, but no previous study has addressed whether limitations to water transport occur beyond this organ or whether dehydration alone can explain shoot impairments. Using common bean (Phaseolus vulgaris) as a model species, we report that waterlogging also impairs water transport in leaves and stems. During the very first hours of waterlogging, leaves transiently dehydrated to water potentials close to the turgor loss point, possibly driving rapid stomatal closure and partially explaining the decline in leaf hydraulic conductance. The initial decline in leaf hydraulic conductance (occurring within 24 h), however, surpassed the levels predicted to occur based solely on dehydration. Constraints to leaf water transport resulted in a hydraulic disconnection between leaves and stems, furthering leaf dehydration during waterlogging and after soil drainage. As leaves dehydrated later during waterlogging, leaf embolism initiated and extensive embolism levels amplified leaf damage. The hydraulic disconnection between leaves and stems prevented stem water potentials from declining below the threshold for critical embolism levels in response to waterlogging. This allowed plants to survive waterlogging and soil drainage. In summary, leaf and stem dehydration are central in defining plant impairments in response to waterlogging, thus creating similarities between waterlogging and drought. Yet, our findings point to the existence of additional players (likely chemicals) partially controlling the early declines in leaf hydraulic conductance and contributing to leaf damage during waterlogging.}, journal={PLANT PHYSIOLOGY}, author={Haverroth, Eduardo J. and Da-Silva, Cristiane J. and Taggart, Matthew and Oliveira, Leonardo A. and Cardoso, Amanda A.}, year={2024}, month={Jun} }
 @misc{martins_da-silva_shabala_striker_carvalho_oliveira_amarante_2024, title={Understanding plant responses to saline waterlogging: insights from halophytes and implications for crop tolerance}, volume={259}, ISSN={["1432-2048"]}, url={https://doi.org/10.1007/s00425-023-04275-0}, DOI={10.1007/s00425-023-04275-0}, number={1}, journal={PLANTA}, author={Martins, Tamires S. and Da-Silva, Cristiane J. and Shabala, Sergey and Striker, Gustavo G. and Carvalho, Ivan R. and Oliveira, Ana Claudia B. and Amarante, Luciano}, year={2024}, month={Jan} }
 @article{shimoia_da-silva_posso_martins_agualongo_oliveira_amarante_2023, title={Co-inoculation of Seeds with <i>Bradyrhizobium</i>,<i>Azospirillum</i>, and<i>Rhizophagus</i>Improves Nitrogen Assimilation and Growth in Soybean Plants Subjected to Waterlogging}, volume={70}, ISSN={["1608-3407"]}, url={http://dx.doi.org/10.1134/s1021443723601040}, DOI={10.1134/S1021443723601040}, number={6}, journal={RUSSIAN JOURNAL OF PLANT PHYSIOLOGY}, publisher={Pleiades Publishing Ltd}, author={Shimoia, E. P. and Da-Silva, C. J. and Posso, D. A. and Martins, T. da Silva and Agualongo, D. A. P. and Oliveira, A. C. B. and Amarante, L.}, year={2023}, month={Dec} }
 @article{boanares_da-silva_costa_filgueira_salles_neto_gastauer_valadares_medeiros_ramos_et al._2023, title={Exogenous Nitric Oxide Alleviates Water Deficit and Increases the Seed Production of an Endemic Amazonian Canga Grass}, volume={24}, ISSN={["1422-0067"]}, url={https://doi.org/10.3390/ijms242316676}, DOI={10.3390/ijms242316676}, abstractNote={Open pit mining can cause loss in different ecosystems, including damage to habitats of rare and endemic species. Understanding the biology of these species is fundamental for their conservation, and to assist in decision-making. Sporobolus multiramosus is an annual grass endemic to the Amazon canga ecosystems, which comprise rocky outcrop vegetation covering one of the world’s largest iron ore reserves. Here, we evaluated whether nitric oxide aids S. multiramosus in coping with water shortages and examined the physiological processes behind these adaptations. nitric oxide application improved the water status, photosynthetic efficiency, biomass production, and seed production and germination of S. multiramosus under water deficit conditions. These enhancements were accompanied by adjustments in leaf and root anatomy, including changes in stomata density and size and root endodermis thickness and vascular cylinder diameter. Proteomic analysis revealed that nitric oxide promoted the activation of several proteins involved in the response to environmental stress and flower and fruit development. Overall, the results suggest that exogenous nitric oxide has the potential to enhance the growth and productivity of S. multiramosus. Enhancements in seed productivity have significant implications for conservation initiatives and can be applied to seed production areas, particularly for the restoration of native ecosystems.}, number={23}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Boanares, Daniela and Da-Silva, Cristiane J. and Costa, Keila Jamille Alves and Filgueira, Joana Patricia Pantoja Serrao and Salles, Marina Ludmila Oliveira Conor and Neto, Luiz Palhares and Gastauer, Markus and Valadares, Rafael and Medeiros, Priscila Sanjuan and Ramos, Silvio Junio and et al.}, year={2023}, month={Dec} }
 @article{silva martins_da-silva_shimoia_posso_carvalho_oliveira_amarante_2023, title={Nitrate supply decreases fermentation and alleviates oxidative and ionic stress in nitrogen-fixing soybean exposed to saline waterlogging}, url={https://doi.org/10.1071/FP22145}, DOI={10.1071/FP22145}, abstractNote={Nitrate (NO3−) nutrition is known to mitigate the damages caused by individual stresses of waterlogging and salinity. Here, we investigated the role of NO3− in soybean plants exposed to these stresses in combination. Nodulated soybean cultivated under greenhouse conditions and daily fertilised with a nutrient solution without nitrogen were subjected to the following treatments: Water, NO3−, NaCl, and NaCl + NO3−. Then, plants were exposed to waterlogging (6 days) and drainage (2 days). Compared to plants exposed to isolated stress, the saline waterlogging resulted in higher concentrations of H2O2, O2˙−, and lipid peroxidation at the whole-plant level, mainly during drainage. Furthermore, saline waterlogging increased fermentation and the concentrations of Na+ and K+ in roots and leaves both during waterlogging and drainage. NO3− supplementation led to augments in NO3− and NO levels, and stimulated nitrate reductase activity in both organs. In addition, NO3− nutrition alleviated oxidative stress and fermentation besides increasing the K+/Na+ ratio in plants exposed to saline waterlogging. In conclusion, NO3− supplementation is a useful strategy to help soybean plants overcome saline waterlogging stress. These findings are of high relevance for agriculture as soybean is an important commodity and has been cultivated in areas prone to saline waterlogging.}, journal={Functional Plant Biology}, author={Silva Martins, Tamires and Da-Silva, Cristiane Jovelina and Shimoia, Eduardo Pereira and Posso, Douglas Antônio and Carvalho, Ivan Ricardo and Oliveira, Ana Claudia Barneche and Amarante, Luciano}, editor={Mathesius, UlrikeEditor}, year={2023}, month={Apr} }
 @article{posso_da-silva_shimoia_martins_reissig_oliveira_borella_dongen_amarante_2023, title={Root-hypoxia tolerance in soybean sister-lines plants indicates a better balance in energy use/dissipation and oxidative stress control}, volume={10}, ISSN={["2667-064X"]}, url={http://dx.doi.org/10.1016/j.stress.2023.100225}, DOI={10.1016/j.stress.2023.100225}, abstractNote={Climatic changes are leading to an increased number of flooding year by year which impacts negatively the metabolism of roots and influences the shoot metabolism which leads to a decline in soybean productivity. This can be more drastic when it is cultivated in lowland soils. Our group hypothesized that tolerant sister-line plants have more capacity to cope with oxidative stress due to better management of energy use and dissipation on photosynthesis machinery during root-flooding and recovery conditions, while in sensitive soybean sister-line the impairment of the photosynthetic use and dissipation of energy lead to an unbalanced redox state response. For that, soybean seeds from the same parents, PELBR15- 7015C (flooding tolerant) and PELBR15-7060 (flooding sensitive) were sowed and cultivated in 500 L capacity plastic containers with lowland soil simulating the field management process. At the reproductive stage, the roots were subjected to flooding for 11 days, then drained to analyze reoxygenation (3 d) and recovery (10 d). Tolerant plants have a greater ability to manage root flooding conditions compared to sensitive ones. The ability includes efficient energy use and dissipation, which minimizes the production of reactive oxygen species (ROS) and subsequent cell damage. Conversely, in sensitive sister lines, impaired electron transport chains lead to increased ROS production and damage. Upon reoxygenation and recovery, the tolerant sister lines exhibit a faster metabolism turnover, allowing them to cope more effectively with the stress on leaves and roots. While both sister lines are capable of detoxifying ROS, the tolerant sister line's superior ability to manage energy use and dissipation allows for more stable and rapid control of oxidative stress.}, journal={PLANT STRESS}, publisher={Elsevier BV}, author={Posso, Douglas Antonio and da-Silva, Cristiane Jovelina and Shimoia, Eduardo Pereira and Martins, Tamires da Silva and Reissig, Gabriela Niemeyer and Oliveira, Ana Claudia Barneche and Borella, Junior and Dongen, Joost Thomas and Amarante, Luciano}, year={2023}, month={Dec} }
 @article{silva_scherner_da-silva_camargo_palacios-zuñiga_schreiber_amarante_parfitt_avila_2022, title={Effect of Water Deficit on Growth and Photoassimilate Partitioning in Leersia hexandra and Luziola peruviana}, volume={12}, ISSN={2077-0472}, url={http://dx.doi.org/10.3390/agriculture12081261}, DOI={10.3390/agriculture12081261}, abstractNote={Minimum tillage in rice production is increasing in southern Brazil, reaching 60% of the total planted area. Compared to conventional tillage, in this system there is an increase in the occurrence of perennial weeds, such as Luziola peruviana and Leersia hexandra, which are amphibious plants, as they can occur in both flooded environments and aerobic conditions. These weeds are controlled in burn-down before rice planting by glyphosate applications, the efficacy of which depends on the soil moisture. This study aimed to evaluate the effect of different soil moisture regimes in L. hexandra and L. peruviana growth and photoassimilate partitioning. Both species were assessed under soil moisture regimes of flooded, saturated, 7 kPa, 15 kPa, 40 kPa, and 100 kPa (experiment 1) and flooded, saturated, and 7 kPa (experiment 2). Plants were sampled immediately after drainage (3 months after transplant) and 1 and 2 weeks after drainage. The results showed that as soil moisture levels decreased, growth and development parameters were negatively affected in both species. Photoassimilate partitioning was affected by water regime and differences were observed between species. The total soluble sugars and starch content decreased in the leaves, and sucrose concentration increased in the roots following drainage. Soil drainage affects the growth and alters carbohydrate partitioning of these weeds, increasing the sugar storage in roots. The agronomic impact of these findings is that this behavior may affect herbicide transport in phloem and may affect herbicide efficacy.}, number={8}, journal={Agriculture}, publisher={MDPI AG}, author={Silva, Renan Souza and Scherner, Ananda and Da-Silva, Cristiane Jovelina and Camargo, Edinalvo Rabaioli and Palacios-Zuñiga, Roque Mauricio and Schreiber, Fabio and Amarante, Luciano do and Parfitt, José Maria Barbat and Avila, Luis Antonio de}, year={2022}, month={Aug}, pages={1261} }
 @article{delias_da-silva_martins_oliveira_amarante_2022, title={Iron toxicity increases oxidative stress and impairs mineral accumulation and leaf gas exchange in soybean plants during hypoxia}, volume={11}, url={https://doi.org/10.1007/s11356-021-17397-3}, DOI={10.1007/s11356-021-17397-3}, journal={Environmental Science and Pollution Research}, publisher={Springer Science and Business Media LLC}, author={Delias, Dominique S. and Da-Silva, Cristiane Jovelina and Martins, Angelita C. and Oliveira, Denise S. C. and Amarante, Luciano}, year={2022}, month={Mar} }
 @inbook{da-silva_amarante_2022, title={Nitric oxide signaling in plants during flooding stress}, url={http://dx.doi.org/10.1016/b978-0-12-818797-5.00009-1}, DOI={10.1016/b978-0-12-818797-5.00009-1}, abstractNote={Flooding is a key limiting condition affecting the growth and productivity of several crop species cultivated in low-lying rainfed areas. During flooding, the oxygen rapidly depletes in the soil, which impairs mitochondrial ATP production and induces NADH accumulation in the roots. Thus, the root metabolism is shifted from aerobic respiration to anaerobic fermentation. Nitric oxide (NO) is an important component of the plant cell signaling conferring increased tolerance against several abiotic stresses, including flooding. In this chapter, we describe the main NO biosynthetic pathways and the functioning of the phytoglobins–NO cycle—an important NO scavenger pathway in plants under low oxygen conditions. We also address the roles of NO in plant tolerance to low oxygen environments such as inducing anaerobic ATP production, NAD+ cycling, oxygen sensing, and aerenchyma formation. Finally, we briefly describe the signaling pathways of NO that result in metabolic and morphological changes in plants under hypoxia and anoxia.}, booktitle={Nitric Oxide in Plant Biology}, publisher={Elsevier}, author={Da-Silva, Cristiane J. and Amarante, Luciano}, year={2022}, pages={241–260} }
 @article{oliveira_da-silva_garcia_agualongo_oliveira_kanamori_takasaki_urano_shinozaki_nakashima_et al._2022, title={The overexpression of NCED results in waterlogging sensitivity in soybean}, volume={3}, url={http://dx.doi.org/10.1016/j.stress.2021.100047}, DOI={10.1016/j.stress.2021.100047}, abstractNote={Soil waterlogging limits plant growth and has a major impact on agricultural productivity. Here, we tested the hypothesis that the overexpression of the NCED gene improves waterlogging and reoxygenation tolerance in soybean plants. This was tested using a transgenic soybean line overexpressing the NCED gene (2Ha11) and the conventional cultivar BRS184 (wild-type; WT), which is the genetic background of the 2Ha11 line. Plants at vegetative (V6) and reproductive (R2) stages were exposed to waterlogging for 60 h and posterior reoxygenation for an additional 60 h. Overall, the NCED overexpression increased the levels of lipid peroxidation in plants exposed to waterlogging and posterior reoxygenation while decreased the antioxidant activity and alcohol dehydrogenase activity. In addition, NCED overexpression decreased seed weight and grain yield in the 2Ha11 line exposed to waterlogging at the reproductive stage. Taken together, these results suggest that the overexpression of NCED triggers an increased waterlogging sensitivity in soybean plants especially when they are exposed to waterlogging at the reproductive stage.}, journal={Plant Stress}, publisher={Elsevier BV}, author={Oliveira, Fabiane Kletke and Da-Silva, Cristiane Jovelina and Garcia, Natália and Agualongo, Darwin Alexis Pomagualli and Oliveira, Ana Cláudia Barneche and Kanamori, Norihito and Takasaki, Hironori and Urano, Kaoru and Shinozaki, Kazuo and Nakashima, Kazuo and et al.}, year={2022}, month={Jan}, pages={100047} }
 @article{agualongo_da-silva_garcia_oliveira_shimoia_posso_oliveira_oliveira_amarante_2022, title={Waterlogging priming alleviates the oxidative damage, carbohydrate consumption, and yield loss in soybean (}, url={https://doi.org/10.1071/FP22030}, DOI={10.1071/FP22030}, abstractNote={In this study, we tested whether waterlogging priming at the vegetative stage would mitigate a subsequent waterlogging event at the reproductive stage in soybean [Glycine max (L.) Merr.]. Plants (V3 stage) were subjected to priming for 7 days and then exposed to waterlogging stress for 5 days (R2 stage) with non-primed plants. Roots and leaves were sampled on the fifth day of waterlogging and the second and fifth days of reoxygenation. Overall, priming decreased the H2O2 concentration and lipid peroxidation in roots and leaves during waterlogging and reoxygenation. Priming also decreased the activity of antioxidative enzymes in roots and leaves and increased the foliar concentration of phenols and photosynthetic pigments. Additionally, priming decreased fermentation and alanine aminotransferase activity during waterlogging and reoxygenation. Finally, priming increased the concentration of amino acids, sucrose, and total soluble sugars in roots and leaves during waterlogging and reoxygenation. Thus, primed plants were higher and more productive than non-primed plants. Our study shows that priming alleviates oxidative stress, fermentation, and carbohydrate consumption in parallel to increase the yield of soybean plants exposed to waterlogging and reoxygenation.}, journal={Functional Plant Biology}, author={Agualongo, Darwin Alexis Pomagualli and Da-Silva, Cristiane Jovelina and Garcia, Natália and Oliveira, Fabiane Kletke and Shimoia, Eduardo Pereira and Posso, Douglas Antônio and Oliveira, Ana Cláudia Barneche and Oliveira, Denise and Amarante, Luciano}, editor={Zeng, FanrongEditor}, year={2022}, month={Aug} }
 @article{braga_de jesus_soares_guatimosim_da silva neto_da-silva_modolo_menezes filho_rhana_cruz_et al._2021, title={A novel H<sub>2</sub>S releasing-monastrol hybrid (MADTOH) inhibits L-type calcium channels}, volume={45}, ISSN={1144-0546 1369-9261}, url={http://dx.doi.org/10.1039/d0nj04415f}, DOI={10.1039/d0nj04415f}, abstractNote={A new alleged monastrol-H2S releasing hybrid, named MADTOH, was designed based on the structure of monastrol (M) and 5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione (ADTOH) and synthesized in 7.8% overall yield.}, number={2}, journal={New Journal of Chemistry}, publisher={Royal Society of Chemistry (RSC)}, author={Braga, Taniris Cafiero and de Jesus, Itamar Couto Guedes and Soares, Kathleen Viveiros and Guatimosim, Silvia and da Silva Neto, Leonardo and da-Silva, Cristiane Jovelina and Modolo, Luzia Valentina and Menezes Filho, José Evaldo Rodrigues and Rhana, Paula and Cruz, Jader Santos and et al.}, year={2021}, pages={671–678} }
 @inbook{da-silva_rodrigues_modolo_2021, title={Hydrogen Sulfide Signaling in the Defense Response of Plants to Abiotic Stresses}, ISBN={9783030736774 9783030736781}, ISSN={2730-6194 2730-6208}, url={http://dx.doi.org/10.1007/978-3-030-73678-1_9}, DOI={10.1007/978-3-030-73678-1_9}, abstractNote={Hydrogen sulfide (H2S) has emerged as a signaling molecule in plants in the late 2000s. Since then, a spectrum of evidence indicates H2S as a key player in plant tolerance to abiotic stresses. This chapter summarizes the production of H2S and its signaling pathways in plants. The main mechanisms of plant defense induced by H2S in response to several abiotic stresses such as high metal availability, high salinity, drought, and extreme temperatures are also highlighted. Finally, the current knowledge on the interplay among H2S, phytohormones, second messengers, and metabolites is also presented. Overall, H2S mitigates the oxidative stress and the damage to organic molecules to maintain seed germination and contribute to plant growth and survival during stressful conditions.}, booktitle={Hydrogen Sulfide and Plant Acclimation to Abiotic Stresses}, publisher={Springer International Publishing}, author={Da-Silva, Cristiane J. and Rodrigues, Ana Cláudia and Modolo, Luzia V.}, year={2021}, pages={139–159} }
 @article{da-silva_shimoia_posso_cardoso_batz_oliveira_amarante_2021, title={Nitrate nutrition increases foliar levels of nitric oxide and waterlogging tolerance in soybean}, volume={43}, url={https://doi.org/10.1007/s11738-021-03291-5}, DOI={10.1007/s11738-021-03291-5}, number={8}, journal={Acta Physiologiae Plantarum}, publisher={Springer Science and Business Media LLC}, author={Da-Silva, Cristiane Jovelina and Shimoia, Eduardo P. and Posso, Douglas A. and Cardoso, Amanda A. and Batz, Timothy A. and Oliveira, Ana Claudia B. and Amarante, Luciano}, year={2021}, month={Aug} }
 @article{canatto_oliveira_da-silva_albino_2021, title={Tolerance of Landoltia punctata to arsenate: an evaluation of the potential use in phytoremediation programs}, volume={23}, url={https://doi.org/10.1080/15226514.2020.1797630}, DOI={10.1080/15226514.2020.1797630}, abstractNote={Abstract Plants used in phytoremediation should accumulate and tolerate a specific pollutant. Here, we aimed at evaluating a possible arsenic (As) accumulation and mechanisms of tolerance against As-induced damage in Landoltia punctata to explore this species for phytoremediation. Plants were subjected to increasing As levels. As absorption was higher with increasing As levels. The activity of superoxide dismutase and glutathione reductase as well as anthocyanin levels increased with As levels. Catalase and peroxidase activities increased in plants subjected to As levels up to 1.0 mg L−1 and decreased at higher levels. Due to the antioxidant system, higher levels of reactive oxygen species were restrained in plants under low levels of As. However, the levels of superoxide anion, hydrogen peroxide, and lipid peroxidation increased in response to the impaired antioxidant system induced by the highest As levels. Biomass decreased in plants exposed to As and scanning electron microscopy revealed root structural damage in the root cap of plants under 3.0 mg L−1 As. This work highlights that L. punctata can be considered a hyperaccumulator species and has potential for As phytoremediation when levels are lower than 1.0 mg L−1—a concentration 100-fold higher than that recommended for drinking water. Novelty Statement: Landoltia punctata can be considered a hyperaccumulator species and has the potential for arsenic phytoremediation when levels are lower than 1.0 mg L−1.}, number={1}, journal={International Journal of Phytoremediation}, publisher={Informa UK Limited}, author={Canatto, Regiane Aparecida and Oliveira, Juraci Alves and da-Silva, Cristiane Jovelina and Albino, Bruno Éric Siqueira}, year={2021}, month={Jan}, pages={102–110} }
 @article{cardoso_gori_da-silva_brunetti_2020, title={Abscisic Acid Biosynthesis and Signaling in Plants: Key Targets to Improve Water Use Efficiency and Drought Tolerance}, volume={10}, url={https://doi.org/10.3390/app10186322}, DOI={10.3390/app10186322}, abstractNote={The observation of a much-improved fitness of wild-type plants over abscisic acid (ABA)-deficient mutants during drought has led researchers from all over to world to perform experiments aiming at a better understanding of how this hormone modulates the physiology of plants under water-limited conditions. More recently, several promising approaches manipulating ABA biosynthesis and signaling have been explored to improve water use efficiency and confer drought tolerance to major crop species. Here, we review recent progress made in the last decade on (i) ABA biosynthesis, (ii) the roles of ABA on plant-water relations and on primary and secondary metabolisms during drought, and (iii) the regulation of ABA levels and perception to improve water use efficiency and drought tolerance in crop species.}, number={18}, journal={Applied Sciences}, publisher={MDPI AG}, author={Cardoso, Amanda A. and Gori, Antonella and Da-Silva, Cristiane J. and Brunetti, Cecilia}, year={2020}, month={Sep}, pages={6322} }
 @article{lima_da-silva_mariot_freitag_serpa_ribeiro_amarante_2020, title={Effect of shading and nitrogen fertilization on nitrogen metabolism, essential oil content and antimicrobial activity of Achillea millefolium}, volume={42}, ISSN={1807-863X 1679-9283}, url={http://dx.doi.org/10.4025/actascibiolsci.v42i1.46412}, DOI={10.4025/actascibiolsci.v42i1.46412}, abstractNote={The Achillea millefolium L. is a perennial herb with important antibacterial, antifungal, anti-inflammatory, antitumoral, and antioxidant properties. This research aimed to investigate the effect of shading (75%; black net) and nitrogen fertilization (0, 75 and 150 kg urea ha-1) on the nitrogen metabolism, essential oil yield and antimicrobial activity of A. millefolium at vegetative- and reproductive-stage. The evaluated parameters varied depending on the organ and the phenological stage of the plant considered. Overall, our findings indicated that shading decreased nitrogen assimilation. Decreased activities of nitrate reductase and glutamine synthetase were observed on shaded plants during reproductive and vegetative stages, respectively. Nitrate and total amino acid levels increased in shaded plants at the vegetative stage. Regarding nitrogen supply, the improved nitrogen metabolism and essential oil yield values were accompanied by intermediate concentrations of urea (75 kg ha-1). Plants fertilized with 75 kg urea ha-1 produced the highest amino acids concentration (vegetative stage), ammonium concentration (vegetative stage) and essential oil yield (reproductive stage). Shading or nitrogen supply did not influence the microbial activity of A. millefolium essential oil. However, the essential oil of leaves and flowers were highly effective against fungi and bacteria, especially gram-positive bacteria. In conclusion, the current study showed that full light and 75 kg urea ha-1 enhanced the nitrogen metabolism of A. millefolium in both vegetative and reproductive stages.}, journal={Acta Scientiarum. Biological Sciences}, publisher={Universidade Estadual de Maringa}, author={Lima, Milene Conceição and Da-Silva, Cristiane Jovelina and Mariot, Marcio Paim and Freitag, Rogério Antônio and Serpa, Rosana and Ribeiro, Gladis Aver and Amarante, Luciano do}, year={2020}, month={Feb}, pages={e46412} }
 @article{boanares_jovelina da-silva_mary dos santos isaias_costa frança_2020, title={Oxidative metabolism in plants from Brazilian rupestrian fields and its relation with foliar water uptake in dry and rainy seasons}, volume={146}, ISSN={0981-9428}, url={http://dx.doi.org/10.1016/j.plaphy.2019.12.005}, DOI={10.1016/j.plaphy.2019.12.005}, abstractNote={In an altitudinal Brazilian ecosystem, fog is a frequent event in both the dry and rainy seasons. The drought stress is aggravated in the dry season due to elevated evaporative demand and this constraint can induce oxidative stress in plants. In this ecosystem, there are plants which present different foliar water uptake (FWU) capacities - species that absorb less water more quickly (LQ) and those that absorb more water more slowly (MS). In this study, the relationship between oxidative stress responses and the different FWU strategies was evaluated in dry and rainy seasons. The oxidative stress was assessed by H2O2 production and lipid peroxidation as well as by the antioxidant enzymes system as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). During the dry season, plants had higher oxidative stress compared to rainy season plants which exhibited moderate oxidative damage. The FWU strategies were closely related to oxidative stress responses, since the LQ species presented the higher H2O2 content and oxidative defense system. Contrastingly, it was found that MS species have the lowest values of H2O2 and less SOD, CAT and APX activities. However, the lipid peroxidation did not present any relation with FWU strategies. Altogether results revealed that plants, which present MS strategy, are more adapted to cope with the higher H2O2 concentrations generated in the dry season and drought stress events than those that present LQ strategy.}, journal={Plant Physiology and Biochemistry}, publisher={Elsevier BV}, author={Boanares, Daniela and Jovelina da-Silva, Cristiane and Mary dos Santos Isaias, Rosy and Costa França, Marcel Giovanni}, year={2020}, month={Jan}, pages={457–462} }
 @article{da-silva_do amarante_2020, title={Short-term nitrate supply decreases fermentation and oxidative stress caused by waterlogging in soybean plants}, volume={176}, ISSN={0098-8472}, url={http://dx.doi.org/10.1016/j.envexpbot.2020.104078}, DOI={10.1016/j.envexpbot.2020.104078}, abstractNote={Waterlogging is one of the main causes of agricultural losses around the world, and nitrate nutrition has been demonstrated to mitigate the negative effects of waterlogging in plants. Here we evaluate the effect of short- and long-term nitrate fertilization in waterlogged nodulated soybean plants and comprehensively examine the mechanisms underpinning nitrate fertilization and soybean tolerance against hypoxia. Waterlogging increased fermentation and oxidative stress and decreased ATP levels in the roots of soybean. Waterlogged plants that received nitrate nutrition (short- or long-term) increased the levels of nitric oxide (NO) and partially restored ATP levels. Also, nitrate nutrition reduced the activity of fermentative and antioxidant enzymes as well as the fermentation substrates and reactive oxygen species (ROS) in waterlogged plants. Few metabolic differences resulted from short- and long-term fertilization, and these differences were not sufficient to impair waterlogging tolerance in soybean plants. By infiltrating roots from waterlogged plants with cPTIO (an NO scavenger), we show that enhanced nitrate nutrition-induced accumulation of NO in waterlogged plants proved to be a key factor for soybean tolerance against waterlogging. Increased ATP accumulation and decreased levels of ROS and toxic products derived from fermentation reactions are suggested as crucial mechanisms underpinning the tolerance of soybean plants under waterlogged conditions.}, journal={Environmental and Experimental Botany}, publisher={Elsevier BV}, author={da-Silva, Cristiane J. and do Amarante, Luciano}, year={2020}, month={Aug}, pages={104078} }
 @article{da-silva_do amarante_2020, title={Time-course biochemical analyses of soybean plants during waterlogging and reoxygenation}, volume={180}, ISSN={0098-8472}, url={http://dx.doi.org/10.1016/j.envexpbot.2020.104242}, DOI={10.1016/j.envexpbot.2020.104242}, abstractNote={Soil waterlogging and subsequent reoxygenation can severely impair soybean (Glycine max) growth and productivity. Here we aimed at showing the main biochemical events that occur in soybean plants during waterlogging and reoxygenation conditions. Roots and leaves were analyzed from the first hour up to ten days of waterlogging and reoxygenation imposition. During the first hours of waterlogging, reactive oxygen species (ROS) and nitric oxide (NO) accumulated in roots and leaves. In roots, only NO levels were persistent, while ROS peaked and decreased over time. In leaves, both ROS and NO increased and remain at high levels during the entire experiment. Despite the increased activity of antioxidant enzymes, malondialdehyde (MDA) levels increased and photosynthetic pigment levels declined. The NO levels increased in response to the increment in nitrate reductase activity in the roots, while in the leaves additional biosynthetic pathways seemed to be involved. The activity of fermentative enzymes and the accumulation of lactate and ethanol occurred after a few hours of waterlogging. Regarding reoxygenation, the transient increase in ROS and NO levels were again observed in roots as well as the increase in MDA levels and antioxidant enzyme activities. In leaves, the levels of ROS and NO decreased as did the activity of the antioxidant enzymes and the MDA levels. In contrast, the levels of photosynthetic pigments increased to pre-waterlogging levels. Both the activity and products of fermentative metabolism decreased. Overall, the biochemical events in soybean plants under waterlogging and reoxygenation conditions are dynamic and change during the time course of stress.}, journal={Environmental and Experimental Botany}, publisher={Elsevier BV}, author={Da-Silva, Cristiane J. and do Amarante, Luciano}, year={2020}, month={Dec}, pages={104242} }
 @article{garcia_da-silva_cocco_pomagualli_de oliveira_da silva_de oliveira_amarante_2020, title={Waterlogging tolerance of five soybean genotypes through different physiological and biochemical mechanisms}, volume={172}, ISSN={0098-8472}, url={http://dx.doi.org/10.1016/j.envexpbot.2020.103975}, DOI={10.1016/j.envexpbot.2020.103975}, abstractNote={Waterlogging is a serious environmental threat that limits crop growth and yield in low-lying, rainfed areas in many regions across the globe. Here we investigated the effects of waterlogging and subsequent re-oxygenation on the physiology and biochemistry of three soybean [Glycine max (L.) Merrill] genotypes (PELBR10-6000, PELBR11-6028, and PELBR11-6042) and two cultivars (TEC IRGA 6070 and BMX Potência). Plants were grown under greenhouse conditions until the V4 stage when they were subjected to waterlogging for seven days. The water was then drained and plants were allowed to recover for another seven days. Overall, all genotypes suppressed waterlogging stress with distinct mechanisms. Waterlogged PELBR10-6000 surpassed control plant levels of CO2 assimilation rate and readily responded to the energy lack induced by hypoxia by activating the fermentative enzymes and alanine aminotransferase. Similar mechanisms were observed in BMX Potência, which restored metabolism to control levels at the end of the recovery. PELBR11-6028 and PELBR11-6042 activated the antioxidant defenses, and TEC IRGA 6070 did not delay flowering.}, journal={Environmental and Experimental Botany}, publisher={Elsevier BV}, author={Garcia, Natália and da-Silva, Cristiane Jovelina and Cocco, Kassia Luiza Teixeira and Pomagualli, Darwin and de Oliveira, Fabiane Kletke and da Silva, João Victor Lemos and de Oliveira, Ana Cláudia Barneche and Amarante, Luciano do}, year={2020}, month={Apr}, pages={103975} }
 @article{duarte_da-silva_marques_modolo_lemos filho_2019, title={Does oxidative stress determine the thermal limits of the regeneration niche of Vriesea friburgensis and Alcantarea imperialis (Bromeliaceae) seedlings?}, volume={80}, ISSN={0306-4565}, url={http://dx.doi.org/10.1016/j.jtherbio.2019.02.003}, DOI={10.1016/j.jtherbio.2019.02.003}, abstractNote={The predicted environmental changes may be detrimental to initial seedling growth, particularly the expected increase in air temperature. We therefore investigated the thermal limits for growth and development of Vriesea friburgensis and Alcantarea imperialis seedlings in the context of oxidative stress. The optimal temperatures for the growth of V. friburgensis and A. imperialis were 25 and 25–30 °C, respectively. Extreme temperatures (15, 30, or 35 °C) induced oxidative stress in both species with significant accumulation of hydrogen peroxide (H2O2) and nitric oxide (NO). Under oxidative stress, the amount of chlorophyll decreased in both species, more prominently in V. friburgensis, while carotenoid levels dramatically increased in A. imperialis. Notably, the activities of superoxide dismutase, catalase (CAT), and ascorbate peroxidase increased in A. imperialis at extreme temperatures. Similar results were observed for V. friburgensis; however, the activity of CAT remained unaffected regardless of temperature. Seedlings of A. imperialis survived at a wider range of temperatures than V. friburgensis, which had greater than 40% mortality when growing at 30 °C. Overall, precise control of cellular H2O2 and NO levels takes place during the establishment of A. imperialis seedlings, allowing the species to cope with relatively high temperatures. The thermal limits of the fundamental niches of the species investigated, determined based on the ability of seedlings to cope with oxidative stress, were distinct from the realized niches of these species. The results suggest that recruitment success is dependent on the ability of seedlings to handle extreme temperature-triggered oxidative stress, which limits the regeneration niche.}, journal={Journal of Thermal Biology}, publisher={Elsevier BV}, author={Duarte, Alexandre Aparecido and da-Silva, Cristiane Jovelina and Marques, Andréa Rodrigues and Modolo, Luzia Valentina and Lemos Filho, José Pires}, year={2019}, month={Feb}, pages={150–157} }
 @inbook{da‐silva_rodrigues_modolo_2019, title={H<sub>2</sub> O<sub>2</sub>, NO, and H<sub>2</sub> S}, url={http://dx.doi.org/10.1002/9781119468677.ch37}, DOI={10.1002/9781119468677.ch37}, abstractNote={Plants produce a variety of reactive oxygen, nitrogen, and sulfur either under physiological conditions or when are threatened. Among them, considerable attention has been directed to hydrogen peroxide (H2O2), nitric oxide (NO), and most recently to hydrogen sulfide (H2S) particularly with respect to their involvement in plant response to abiotic stresses. This chapter highlights the main sources of these signaling molecules, the control of their homeostasis, the interaction among them, and biochemical events subsequently triggered in plant cells in response to several abiotic stress. Herein, it is intended to show the way H2O2, NO, and H2S tailor to fancily suit plants against environmental adverse conditions.}, booktitle={Reactive Oxygen, Nitrogen and Sulfur Species in Plants}, publisher={Wiley}, author={da‐Silva, Cristiane J. and Rodrigues, Ana Claudia and Modolo, Luzia V.}, year={2019}, month={Jul}, pages={841–856} }
 @inbook{souri_cardoso_da‐silva_oliveira_dari_sihi_karimi_2019, title={Heavy Metals and Photosynthesis: Recent Developments}, url={http://dx.doi.org/10.1002/9781119501800.ch7}, DOI={10.1002/9781119501800.ch7}, abstractNote={Heavy metals are among the main pollutants affecting plant photosynthesis. A broad literature screening reveals that heavy metals impair, in a type- and dose-dependent manner, many aspects related to the photosynthetic apparatus. This chapter explores how stomatal and mesophyll conductances, chloroplasts, photosynthetic pigments, photosystems I and II, photosynthetic enzymes, and the antioxidant defense mechanism are negatively affected by heavy metals. It also describes how hyperaccumulator plants cope with potential disturbances in photosynthesis upon heavy metal stress.}, booktitle={Photosynthesis, Productivity and Environmental Stress}, publisher={Wiley}, author={Souri, Zahra and Cardoso, Amanda A. and da‐Silva, Cristiane J. and Oliveira, Letúzia M. and Dari, Biswanath and Sihi, Debjani and Karimi, Naser}, year={2019}, month={Sep}, pages={107–134} }
 @misc{chaves-silva_da silva_da-silva_2019, title={Molecular Biology Tools to Boost the Production of Natural Products}, url={http://dx.doi.org/10.1201/b22296-4}, DOI={10.1201/b22296-4}, abstractNote={The determination of the DNA structure in the 1950s offered a new way for scientists to look at living organisms. Subsequent genomics studies were a breakthrough in the investigation of the organisms' physiology. The interactions of an organism's genes with one another and how the expression of genes is affected by the surrounding environment can be better understood. Several techniques of gene and genome editing have been developed in the last few decades for multiple purposes. Specifically, we can investigate in a rational manner, mechanisms of biosynthetic pathways and target the accumulation of valuable substances in cells or suppression of undesired compounds. This chapter presents the concepts behind forward and reverse genetics and synthetic biology. Herein, we provide examples of genome editing techniques looking at gene/genome editing of medicinal plants. How such technologies might be useful to produce natural products of Brazilian medicinal plants origin are highlighted.}, journal={Brazilian Medicinal Plants}, publisher={CRC Press}, author={Chaves-Silva, Samuel and da Silva, Thamara Ferreira and da-Silva, Cristiane Jovelina}, editor={Modolo, Luzia ValentinaEditor}, year={2019}, month={Nov}, pages={71–90} }
 @article{plants of achillea millefolium l. grown under colored shading nets have altered secondary metabolism_2019, journal={Brazilian Journal of Biosciences}, year={2019}, month={Dec} }
 @article{modolo_da-silva_brandão_chaves_2018, title={A minireview on what we have learned about urease inhibitors of agricultural interest since mid-2000s}, volume={13}, ISSN={2090-1232}, url={http://dx.doi.org/10.1016/j.jare.2018.04.001}, DOI={10.1016/j.jare.2018.04.001}, abstractNote={World population is expected to reach 9.7 billion by 2050, which makes a great challenge the achievement of food security. The use of urease inhibitors in agricultural practices has long been explored as one of the strategies to guarantee food supply in enough amounts. This is due to the fact that urea, one of the most used nitrogen (N) fertilizers worldwide, rapidly undergoes urease-driven hydrolysis on soil surface yielding up to 70% N losses to environment. This review provides with a compilation of what has been done since 2005 with respect to the search for good urease inhibitors of agricultural interests. The potential of synthetic organic molecules, such as phosphoramidates, hydroquinone, quinones, (di)substituted thioureas, benzothiazoles, coumarin and phenolic aldehyde derivatives, and vanadium-hydrazine complexes, together with B, Cu, S, Zn, ammonium thiosulfate, silver nanoparticles, and oxidized charcoal as urease inhibitors was presented from experiments with purified jack bean urease, different soils and/or plant-soil systems. The ability of some urease inhibitors to mitigate formation of greenhouse gases is also discussed.}, journal={Journal of Advanced Research}, publisher={Elsevier BV}, author={Modolo, Luzia V. and da-Silva, Cristiane J. and Brandão, Débora S. and Chaves, Izabel S.}, year={2018}, month={Sep}, pages={29–37} }
 @article{das_silva_silva_dantas_fátima_góis ruiz_silva_carvalho_santos_figueiredo_et al._2018, title={Highly functionalized piperidines: Free radical scavenging, anticancer activity, DNA interaction and correlation with biological activity}, volume={9}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85032893999&partnerID=MN8TOARS}, DOI={10.1016/j.jare.2017.10.010}, abstractNote={Twenty-five piperidines were studied as potential radical scavengers and antitumor agents. Quantitative interaction of compounds with ctDNA using spectroscopic techniques was also evaluated. Our results demonstrate that the evaluated piperidines possesses different abilities to scavenge the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the anion radical superoxide (O2−). The piperidine 19 was the most potent radical DPPH scavenger, while the most effective to O2− scavenger was piperidine 10. In general, U251, MCF7, NCI/ADR-RES, NCI-H460 and HT29 cells were least sensitive to the tested compounds and all compounds were considerably more toxic to the studied cancer cell lines than to the normal cell line HaCaT. The binding mode of the compounds and ctDNA was preferably via intercalation. In addition, these results were confirmed based on theoretical studies. Finally, a linear and exponential correlation between interaction constant (Kb) and GI50 for several human cancer cell was observed.}, journal={Journal of Advanced Research}, author={Das, S. and Silva, C.J. and Silva, M.D.M. and Dantas, M.D.D.A. and Fátima, Â. and Góis Ruiz, A.L.T. and Silva, C.M. and Carvalho, J.E. and Santos, J.C.C. and Figueiredo, I.M. and et al.}, year={2018}, pages={51–61} }
 @article{da-silva_modolo_2018, title={Hydrogen sulfide: A new endogenous player in an old mechanism of plant tolerance to high salinity}, volume={32}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85040764662&partnerID=MN8TOARS}, DOI={10.1590/0102-33062017abb0229}, abstractNote={High salinity aff ects plants due to stimulation of osmotic stress. Cell signaling triggered by nitric oxide (NO) and hydrogen sulfi de (H2S) activates a cascade of biochemical events that culminate in plant tolerance to abiotic and biotic stresses. For instance, the NO/H2S-stimulated biochemical events that occur in plants during response to high salinity include the control of reactive oxygen species, activation of antioxidant system, accumulation of osmoprotectants in cytosol, induction of K+ uptake and Na+ cell extrusion or its vacuolar compartmentation among others. Th is review is a compilation of what we have learned in the last 10 years about NO participation during cell signaling in response to high salinity as well as the role of H2S, a new player in the mechanism of plant tolerance to salt stress. Th e main sources of NO and H2S in plant cells is also discussed together with the evidence of interplay between both signaling molecules during response to stress.}, number={1}, journal={Acta Botanica Brasilica}, author={da-Silva, C.J. and Modolo, L.V.}, year={2018}, pages={150–160} }
 @article{da-silva_mollica_vicente_peres_modolo_2018, title={NO, hydrogen sulfide does not come first during tomato response to high salinity}, volume={76}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85030754040&partnerID=MN8TOARS}, DOI={10.1016/j.niox.2017.09.008}, abstractNote={High salinity greatly impacts agriculture, particularly in tomato (Solanum lycopersicum), a crop that is a model to study this abiotic stress. This work investigated whether hydrogen sulfide (H2S) acts upstream or downstream of nitric oxide (NO) in the signaling cascade during tomato response to salt stress. An NO-donor incremented H2S levels by 12-18.9% while an H2S-donor yielded 10% more NO in roots. The NO accumulated in roots one-hour after NaCl treatment while H2S accumulation started two-hour later. The NO stimulated H2S accumulation in roots/leaves, but not the opposite (i.e H2S was unable to stimulate NO accumulation) two-hour post NaCl treatment. Also, NO accumulation was accompanied by an increment of transcript levels of genes that encode for H2S-synthesizing enzymes. Our results indicate that H2S acts downstream of NO in the mitigation of oxidative stress, which helps tomato plants to tolerate high salinity.}, journal={Nitric Oxide - Biology and Chemistry}, author={da-Silva, C.J. and Mollica, D.C.F. and Vicente, M.H. and Peres, L.E.P. and Modolo, L.V.}, year={2018}, pages={164–173} }
 @article{da-silva_canatto_cardoso_ribeiro_de oliveira_2018, title={Oxidative stress triggered by arsenic in a tropical macrophyte is alleviated by endogenous and exogenous nitric oxide}, volume={41}, ISSN={0100-8404 1806-9959}, url={http://dx.doi.org/10.1007/s40415-017-0431-y}, DOI={10.1007/s40415-017-0431-y}, abstractNote={Nitric oxide (NO) plays important role in alleviating abiotic stresses in plants, including those caused by arsenic (As). Here, we examined the effects of endogenous and exogenous NO in Spirodela intermedia W. Koch (Lemnaceae) under As exposure. For this purpose, we evaluated the As content, reactive oxygen species (ROS) levels, membrane damage and enzymatic antioxidant system. The levels of endogenous NO and the activity of nitrate reductase (NR) were also addressed. The As treatment triggered the production of high endogenous levels of NO and a pronounced activation of the antioxidant enzymes; however, it was not sufficient to completely avoid the increment in ROS content and membrane damage. In contrast, exogenous NO decreased the As levels in plants exposed to As and NO donor, mitigating the ROS production and membrane damage, while maintaining a lower activity of the antioxidant enzymes compared with As-treated plants. Exogenous NO further downregulated the NR activity by a negative feedback, while As boosted the NR activity, consistent with the high endogenous levels of NO observed upon As treatment. Our results suggest that both endogenous and exogenous NO play critical roles in alleviating the As-induced oxidative stress in S. intermedia by reducing As uptake, and possibly by acting as an antioxidant molecule.}, number={1}, journal={Brazilian Journal of Botany}, publisher={Springer Science and Business Media LLC}, author={da-Silva, Cristiane Jovelina and Canatto, Regiane Aparecida and Cardoso, Amanda Avila and Ribeiro, Cleberson and de Oliveira, Juraci Alves}, year={2018}, pages={21–28} }
 @article{da-silva_canatto_cardoso_ribeiro_oliveira_2017, title={Arsenic-hyperaccumulation and antioxidant system in the aquatic macrophyte Spirodela intermedia W. Koch (Lemnaceae)}, volume={29}, url={https://doi.org/10.1007/s40626-017-0096-8}, DOI={10.1007/s40626-017-0096-8}, number={4}, journal={Theoretical and Experimental Plant Physiology}, publisher={Springer Nature}, author={da-Silva, Cristiane J. and Canatto, Regiane A. and Cardoso, Amanda A. and Ribeiro, Cleberson and Oliveira, Juraci A.}, year={2017}, month={Dec}, pages={203–213} }
 @article{liberto_simï??es_paiva silva_silva_modolo_fï??tima_silva_derita_zacchino_zuï??iga_et al._2017, title={Quinolines: Microwave-assisted synthesis and their antifungal, anticancer and radical scavenger properties}, volume={25}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85009431717&partnerID=MN8TOARS}, DOI={10.1016/j.bmc.2016.12.023}, abstractNote={An efficient method for the synthesis of quinolines using microwave irradiation was developed providing 28 quinolines with good yields. The reaction procedures are environmentally friendly, convenient, mild and of easy work-up. Quinolines were evaluated for their antifungal, anticancer and antioxidant properties and exhibited high activities in all tests performed.}, number={3}, journal={Bioorganic and Medicinal Chemistry}, author={Liberto, N.A. and Simï??es, J.B. and Paiva Silva, S. and Silva, C.J. and Modolo, L.V. and Fï??tima, ï??. and Silva, L.M. and Derita, M. and Zacchino, S. and Zuï??iga, O.M.P. and et al.}, year={2017}, pages={1153–1162} }
 @article{silva_batista fontes_modolo_2017, title={Salinity-induced accumulation of endogenous H<inf>2</inf>S and NO is associated with modulation of the antioxidant and redox defense systems in Nicotiana tabacum L. cv. Havana}, volume={256}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85009085828&partnerID=MN8TOARS}, DOI={10.1016/j.plantsci.2016.12.011}, abstractNote={Salinity is one of the abiotic factors that most affect crop growth and production. This study focused on the effect of high salinity on the endogenous levels of the signaling molecules hydrogen sulfite (H2S) and nitric oxide (NO) in Nicotiana tabacum leaves and the extent of these for the biochemically-driven plant tolerance to such abiotic stress. The NaCl treatment for 10 days led to an expressive augment of H2S and NO levels. This increase was correlated with the raise of l-Cys and l-Arg and the induction of l-cysteine desulfhydrase, cyanoalanine synthase, cysteine synthase, nitrate reductase and arginase, enzymes known to be involved in the biosynthesis of H2S or NO. The enzymatic antioxidant system (superoxide dismutase and catalase activity) was boosted and the non-enzymatic antioxidant glutathione was intensively oxidized in leaves upon stress allowing plants to cope with oxidative stress. Lower stomatal conductance was observed in stressed plants in comparison with control ones. Moreover, the high activity of antioxidant enzymes and high rate of glutathione oxidation following salt stress were considerably decreased upon NO or H2S scavenging. Thus, increment in NO and H2S levels and their interplay, along with metabolic and physiological changes, contributed to tobacco survival to extreme salinity conditions.}, journal={Plant Science}, author={Silva, C.J. and Batista Fontes, E.P. and Modolo, L.V.}, year={2017}, pages={148–159} }
 @article{resende_braga_pereira_da silva_vale_bianchetti_forzza_ribeiro_peixoto_2016, title={Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)}, volume={76}, ISSN={1678-4375 1519-6984}, url={http://dx.doi.org/10.1590/1519-6984.19314}, DOI={10.1590/1519-6984.19314}, abstractNote={Abstract This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA3 or 0.2 µM NAA, sucrose at 15 or 30 g L–1, in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA3 and 15 g L–1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L–1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.}, number={1}, journal={Brazilian Journal of Biology}, publisher={FapUNIFESP (SciELO)}, author={Resende, C. F. and Braga, V. F. and Pereira, P. F. and da Silva, C. J. and Vale, V. F. and Bianchetti, R. E. and Forzza, R. C. and Ribeiro, C. and Peixoto, P. H. P.}, year={2016}, month={Jan}, pages={218–227} }
 @article{cardoso_obolari_borges_silva_rodrigues_m. m. obolari_borges_silva_2015, title={Environmental factors on seed germination, seedling survival and initial growth of sacha inchi (Plukenetia volubilis l.),Fatores ambientais na germinação de sementes e na sobrevivência e crescimento inicial de plântulas de sacha inchi (Plukenetia volubilis L.)}, volume={37}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84937002825&partnerID=MN8TOARS}, DOI={10.1590/2317-1545v37n2145054}, abstractNote={Abstract: Sacha inchi (Plukenetia volubilis L.) is an Amazon species of elevated agro-industrial potential due the high content of omega-3 and omega-6 in its seeds. Despite of it, little information about its propagation by seeds is currently available. Thus, the aim of this study was to assess seed germination, seedling survival and growth of this species under different conditions of substrate (on paper, between papers and paper roll), light (continuous darkness, 12-h photoperiod and continuous light) and temperature (continuous temperature at 20, 25, 30, 35 and 40 °C). Germination is stimulated by substrates with increased surface contact with the seeds, presence of light and temperatures between 25 and 35 °C. Survival and initial growth of seedlings are favored by vermiculite, continuous light and 30 °C temperature. These conditions allow rapid and uniform germination of seeds and better establishment and development of seedlings. We encourage the propagation of sacha inchi by seeds, since we consider it a feasible technique.}, number={2}, journal={Journal of Seed Science}, author={Cardoso, A. A. and Obolari, A. de M. M. and Borges, E. E. de and Silva, C. J. da and Rodrigues, H. S. and M. M. Obolari, A. and Borges, E. E. and Silva, C. J.}, year={2015}, pages={111–116} }
 @misc{modolo_da silva_da silva_da silva neto_de fátima_2015, title={Introduction to the Biosynthesis and Biological Activities of Phenylpropanoids}, url={http://dx.doi.org/10.1002/9783527684403.ch14}, DOI={10.1002/9783527684403.ch14}, abstractNote={The sessile nature of plants made these organisms evolve biosynthetic routes that culminate in the production of secondary metabolites that are used to cope with stressful environmental stimuli. Phenylpropanoids compounds, which bear a C6–C3 phenolic scaffold, have received particular attention not only because of their function in plants but also because of their wide spectrum of biological activities. This chapter provides information about the biosynthesis of phenylpropanoid compounds. The main focus of this compilation is the subclass of phenylpropanoids named flavonoids, coumarins, and stilbenes. Examples of substances that belong to such subclasses are given together with a parallel between the function of such phenylpropanoid classes in plants and animals. In this sense, we intend to cover the notable biological activities exhibited by phenylpropanoids that have been used as inspiration for the design of new lead compounds for a variety of therapeutic use.}, journal={Bioactive Natural Products}, publisher={Wiley-VCH Verlag GmbH & Co. KGaA}, author={Modolo, Luzia V. and da Silva, Cristiane J. and da Silva, Fernanda G. and da Silva Neto, Leonardo and de Fátima, Ângelo}, year={2015}, month={Jul}, pages={387–408} }
 @article{cardoso_borges_souza_silva_pires_dias_2015, title={Seed imbibition and germination of Plothymenia reticulata Benth. (Fabaceae) affected by mercury: Possible role of aquaporins}, volume={29}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84942324027&partnerID=MN8TOARS}, DOI={10.1590/0102-33062014abb0038}, abstractNote={Studies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants.}, number={3}, journal={Acta Botanica Brasilica}, author={Cardoso, A.?. and Borges, E.E.L. and Souza, G.A. and Silva, C.J. and Pires, R.M.O. and Dias, D.C.F.S.}, year={2015}, pages={285–291} }
 @article{resende_braga_silva_pereira_ribeiro_salimena_peixoto_2014, title={An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae)}, volume={28}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84901000822&partnerID=MN8TOARS}, DOI={10.1590/S0102-33062014000200005}, abstractNote={This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervao-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 µM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 µM). The medium found to induce the greatest number of shoot was that containing 5 µM of BA (NAA-free). For rooting, we evaluated three auxins (NAA, indole-3-acetic acid and indole-3-butyric acid; 0.1, 0.2, 0.3 and 0.4 µM), as well as a control. No differences were observed between the control and the other treatments. The auxin-free medium was deemed the most suitable, because it ensures the lowest cost in the micropropagation procedures. We obtained 100% survival of the acclimatized seedlings, and the plants showed normal vegetative and reproductive development, suggesting that the micropropagation did not alter the biological cycle of this species. The results show the importance and potential of micropropagation for biodiversity conservation of Bouchea fluminensis.}, number={2}, journal={Acta Botanica Brasilica}, author={Resende, C.F. and Braga, V.F. and Silva, C.J. and Pereira, P.F. and Ribeiro, C. and Salimena, F.R.G. and Peixoto, P.H.P.}, year={2014}, pages={184–189} }