@article{willman_bushakra_bassil_finn_dossett_perkins-veazie_bradish_fernandez_weber_scheerens_et al._2022, title={Analysis of a Multi-Environment Trial for Black Raspberry (Rubus occidentalis L.) Quality Traits}, volume={13}, ISSN={2073-4425}, url={http://dx.doi.org/10.3390/genes13030418}, DOI={10.3390/genes13030418}, abstractNote={U.S. black raspberry (BR) production is currently limited by narrowly adapted, elite germplasm. An improved understanding of genetic control and the stability of pomological traits will inform the development of improved BR germplasm and cultivars. To this end, the analysis of a multiple-environment trial of a BR mapping population derived from a cross that combines wild ancestors introgressed with commercial cultivars on both sides of its pedigree has provided insights into genetic variation, genotype-by-environment interactions, quantitative trait loci (QTL), and QTL-by-environment interactions (QEI) of fruit quality traits among diverse field environments. The genetic components and stability of four fruit size traits and six fruit biochemistry traits were characterized in this mapping population following their evaluation over three years at four distinct locations representative of current U.S. BR production. This revealed relatively stable genetic control of the four fruit size traits across the tested production environments and less stable genetic control of the fruit biochemistry traits. Of the fifteen total QTL, eleven exhibited significant QEI. Closely overlapping QTL revealed the linkage of several fruit size traits: fruit mass, drupelet count, and seed fraction. These and related findings are expected to guide further genetic characterization of BR fruit quality, management of breeding germplasm, and development of improved BR cultivars for U.S. production.}, number={3}, journal={Genes}, publisher={MDPI AG}, author={Willman, Matthew R. and Bushakra, Jill M. and Bassil, Nahla and Finn, Chad E. and Dossett, Michael and Perkins-Veazie, Penelope and Bradish, Christine M. and Fernandez, Gina E. and Weber, Courtney A. and Scheerens, Joseph C. and et al.}, year={2022}, month={Feb}, pages={418} }
 @article{baptissart_bradish_jones_walsh_tehrani_marrero-colon_mehta_jima_oh_diehl_et al._2022, title={Zac1 and the Imprinted Gene Network program juvenile NAFLD in response to maternal metabolic syndrome}, volume={2}, ISSN={["1527-3350"]}, url={https://doi.org/10.1002/hep.32363}, DOI={10.1002/hep.32363}, abstractNote={Background and Aims Within the next decade, NAFLD is predicted to become the most prevalent cause of childhood liver failure in developed countries. Predisposition to juvenile NAFLD can be programmed during early life in response to maternal metabolic syndrome (MetS), but the underlying mechanisms are poorly understood. We hypothesized that imprinted genes, defined by expression from a single parental allele, play a key role in maternal MetS-induced NAFLD, due to their susceptibility to environmental stressors and their functions in liver homeostasis. We aimed to test this hypothesis and determine the critical periods of susceptibility to maternal MetS. Approach and Results We established a mouse model to compare the effects of MetS during prenatal and postnatal development on NAFLD. Postnatal but not prenatal MetS exposure is associated with histological, biochemical, and molecular signatures of hepatic steatosis and fibrosis in juvenile mice. Using RNA sequencing, we show that the Imprinted Gene Network (IGN), including its regulator Zac1, is up-regulated and overrepresented among differentially expressed genes, consistent with a role in maternal MetS-induced NAFLD. In support of this, activation of the IGN in cultured hepatoma cells by overexpressing Zac1 is sufficient to induce signatures of profibrogenic transformation. Using chromatin immunoprecipitation, we demonstrate that Zac1 binds the TGF-β1 and COL6A2 promoters, forming a direct pathway between imprinted genes and well-characterized pathophysiological mechanisms of NAFLD. Finally, we show that hepatocyte-specific overexpression of Zac1 is sufficient to drive fibrosis in vivo. Conclusions Our findings identify a pathway linking maternal MetS exposure during postnatal development to the programming of juvenile NAFLD, and provide support for the hypothesis that imprinted genes play a central role in metabolic disease programming.}, journal={HEPATOLOGY}, author={Baptissart, Marine and Bradish, Christine M. and Jones, Brie S. and Walsh, Evan and Tehrani, Jesse and Marrero-Colon, Vicmarie and Mehta, Sanya and Jima, Dereje D. and Oh, Seh Hoon and Diehl, Anna Mae and et al.}, year={2022}, month={Feb} }
 @article{baptissart_lamb_to_bradish_tehrani_reif_cowley_2018, title={Neonatal mice exposed to a high-fat diet in utero influence the behaviour of their nursing dam}, volume={285}, url={https://doi.org/10.1098/rspb.2018.1237}, DOI={10.1098/rspb.2018.1237}, abstractNote={The behaviour of a nursing dam influences the development, physiology, and behaviour of her offspring. Maternal behaviours can be modulated both by environmental factors, including diet, and by physical or behavioural characteristics of the offspring. In most studies of the effects of the environment on maternal behaviour, F 0 dams nurse their own F 1 offspring. Because the F 1 are indirectly exposed to the environmental stressor in utero in these studies, it is not possible to differentiate between effects on maternal behaviour from direct exposure of the dam and those mediated by changes in the F 1 as a consequence of in utero exposure. In this study, we used a mouse model of high-fat (HF) diet feeding, which has been shown to influence maternal behaviours, combined with cross-fostering to discriminate between these effects. We tested whether the diet of the F 0 dam or the exposure experienced by the F 1 pups in utero is the most significant predictor of maternal behaviour. Neither factor significantly influenced pup retrieval behaviours. However, strikingly, F 1 in utero exposure was a significant predictor of maternal behaviour in the 15 min immediately following pup retrieval while F 0 diet had no discernable effect. Our findings suggest that in utero exposure to HF diet programmes physiological changes in the offspring which influence the maternal behaviours of their dam after birth.}, number={1891}, journal={Proceedings of the Royal Society B: Biological Sciences}, publisher={The Royal Society}, author={Baptissart, Marine and Lamb, Harold E. and To, Kimberly and Bradish, Christine and Tehrani, Jesse and Reif, David and Cowley, Michael}, year={2018}, month={Nov}, pages={20181237} }
 @article{perkins-veazie_ma_fernandez_bradish_bushakra_bassil_weber_scheerens_robbins_finn_et al._2016, title={Black raspberry fruit composition over two years from seedling populations grown at four US geographic locations}, volume={1133}, ISSN={0567-7572 2406-6168}, url={http://dx.doi.org/10.17660/actahortic.2016.1133.52}, DOI={10.17660/actahortic.2016.1133.52}, number={1133}, journal={Acta Horticulturae}, publisher={International Society for Horticultural Science (ISHS)}, author={Perkins-Veazie, P. and Ma, G. and Fernandez, G.E. and Bradish, C.M. and Bushakra, J.M. and Bassil, N.V. and Weber, C.A. and Scheerens, J.C. and Robbins, L. and Finn, C.E. and et al.}, year={2016}, month={May}, pages={335–338} }
 @article{bradish_overbaugh_ballington_fernandez_bassil_2016, title={Comparative diversity analysis of southeastern Rubus germplasm through molecular and pedigree techniques}, volume={1127}, ISSN={["2406-6168"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85007394056&partnerID=MN8TOARS}, DOI={10.17660/actahortic.2016.1127.25}, journal={XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): INTERNATIONAL SYMPOSIUM ON PLANT BREEDING IN HORTICULTURE}, author={Bradish, C. M. and Overbaugh, E. and Ballington, J. and Fernandez, G. E. and Bassil, N. V.}, year={2016}, pages={157–161} }
 @article{bradish_fernandez_bushakra_perkins-veazie_dossett_bassil_finn_2016, title={Evaluation of vigor and winter hardiness of black raspberry breeding populations (Rubus occidentalis) grown in the southeastern US}, volume={1133}, ISSN={["2406-6168"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84973606487&partnerID=MN8TOARS}, DOI={10.17660/actahortic.2016.1133.19}, abstractNote={Demand for fresh berry products continues to grow in the United States; however, commercial raspberry production in the southeastern US is difficult because cultivars are not well adapted to the warm climate and fluctuating winter temperatures, where heat degrades plant vigor and fruit quality, and warm spells in mid to late winter can cause low chilling plants to break bud too early. Recent research about the health benefits of a polyphenolic-rich diet has led to a resurgence of interest and breeding efforts in black raspberry, and is the driving factor for this particular research. Two half-sib populations, ORUS 4304 and ORUS 4305 (307 progeny total) were planted in Jackson Springs, North Carolina in 2012. From fall 2012-spring 2015, a number of traits were measured on each plant, among them vigor, winter hardiness, and heat damage, analyzed in several different ways. Plant vigor increased or decreased over harvest seasons based on individual differences in the segregating plant population, but also showed correlations with temperature, i.e., less vigorous plants were more susceptible to damage by heat or cold. Overall, ORUS 4305 was less vigorous than ORSU 4304. As is the case with most raspberries grown in the southeast, vigor in general is expected to decrease over time; however, certain individuals show transgressive segregation for the trait. Linkage mapping for both populations is underway to identify and validate QTL for vigor, winter hardiness, heat tolerance, and other traits within black raspberry. Ultimately, we hope to gain a more complete understanding of black raspberry physiology and genetics to make breeding commercial quality cultivars a possibility in the southeast.}, number={1133}, journal={XI INTERNATIONAL RUBUS AND RIBES SYMPOSIUM}, publisher={International Society for Horticultural Science (ISHS)}, author={Bradish, C. M. and Fernandez, G. E. and Bushakra, J. M. and Perkins-Veazie, P. and Dossett, M. and Bassil, N. V. and Finn, C. E.}, year={2016}, pages={129–133} }
 @article{bradish_bushakra_dossett_bassil_finn_fernandez_2016, title={Genotyping and phenotyping heat tolerance in black raspberry (Rubus occidentalis L.)}, volume={1127}, ISSN={["2406-6168"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85007373316&partnerID=MN8TOARS}, DOI={10.17660/actahortic.2016.1127.50}, number={1127}, journal={XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): INTERNATIONAL SYMPOSIUM ON PLANT BREEDING IN HORTICULTURE}, publisher={International Society for Horticultural Science (ISHS)}, author={Bradish, C. M. and Bushakra, J. M. and Dossett, M. and Bassil, N. V. and Finn, C. E. and Fernandez, G. E.}, year={2016}, pages={321–324} }
 @article{bushakra_bradish_weber_dossett_fernandez_weiland_peterson_scheerens_robbins_serce_et al._2016, title={Toward understanding genotype x environment interactions in black raspberry (Rubus occidentalis L.)}, volume={1117}, ISSN={["0567-7572"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84971528876&partnerID=MN8TOARS}, DOI={10.17660/actahortic.2016.1117.5}, number={1117}, journal={XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): II INTERNATIONAL BERRY FRUIT SYMPOSIUM: INTERACTIONS! LOCAL AND GLOBAL BERRY RESEARCH AND INNOVATION}, publisher={International Society for Horticultural Science (ISHS)}, author={Bushakra, J. M. and Bradish, C. M. and Weber, C. A. and Dossett, M. and Fernandez, G. and Weiland, J. and Peterson, M. and Scheerens, J. C. and Robbins, L. and Serce, S. and et al.}, year={2016}, pages={25–29} }
 @article{bradish_yousef_ma_perkins-veazie_fernandez_2015, title={Anthocyanin, carotenoid, tocopherol, and ellagitannin content of red raspberry cultivars grown under field or high tunnel cultivation in the Southeastern United States}, volume={140}, number={2}, journal={Journal of the American Society for Horticultural Science}, author={Bradish, C. M. and Yousef, G. G. and Ma, G. Y. and Perkins-Veazie, P. and Fernandez, G. E.}, year={2015}, pages={163–171} }
 @article{bradis_yousef_ma_perkins-veazie_fernandez_2015, title={Anthocyanin,Carotenoid, Tocopherol, Andellagitannin Content of Red Raspberry Cultivars Grown Under Field or High Tunnel Cultivation in the Southeastern United States}, volume={140}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84927935205&partnerID=MN8TOARS}, number={2}, journal={Journal of the American Society for Horticultural Science}, author={Bradis, C.M. and Yousef, G.G. and Ma, G. and Perkins-Veazie, P. and Fernandez, G.E.}, year={2015}, pages={163–171} }
 @article{bradish_perkins-veazie_fernandez_xie_jia_2012, title={Comparison of Flavonoid Composition of Red Raspberries (Rubus idaeus L.) Grown in the Southern United States}, volume={60}, ISSN={["1520-5118"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84862185617&partnerID=MN8TOARS}, DOI={10.1021/jf203474e}, abstractNote={Raspberry flavonoid compounds have significant antioxidant activities, and regular consumption may help prevent and/or moderate chronic diseases. Targeted metabolite profiling is useful to identify compounds contributing to these antioxidant properties and health benefits and for tailored breeding for functional foods. In this study, metabolomic variation was determined among three fall-fruiting red raspberry cultivars (‘Autumn Britten’, ‘Caroline’, ‘Nantahala’) grown at three North Carolina locations differing in elevation and average day/night temperatures. ‘Nantahala’ was specifically bred for the mountainous regions of the southern United States. Ten flavonoid compounds were detected by liquid chromatography–time-of-flight–mass spectrometry (LC-TOF-MS). Of those, cyanidin-3-glucoside, cyanidin-3-sophoroside, cyanidin-3-rutinoside, cyanidin-3-sambubioside, and quercetin-3-glucoside were quantified against external standards. Variation in flavonoid composition was primarily attributed to genotype and associated with night temperature and hours exposed to temperatures over 29 °C. ‘Nantahala’ had particularly high levels of cyanidin-3-sambubioside, indicative of its purple raspberry lineage. Quercetin-3-glucoside levels increased the most with elevated temperatures.}, number={23}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Bradish, Christine M. and Perkins-Veazie, Penelope and Fernandez, Gina E. and Xie, Guoxiang and Jia, Wei}, year={2012}, month={Jun}, pages={5779–5786} }