@article{maier_gin_callahan_sheriff_duerkop_kleiner_2024, title={Pseudo-pac site sequences used by phage P22 in generalized transduction of <i>Salmonella</i>}, volume={20}, ISSN={["1553-7374"]}, url={https://doi.org/10.1371/journal.ppat.1012301}, DOI={10.1371/journal.ppat.1012301}, abstractNote={Salmonella enterica Serovar Typhimurium ( Salmonella ) and its bacteriophage P22 are a model system for the study of horizontal gene transfer by generalized transduction. Typically, the P22 DNA packaging machinery initiates packaging when a short sequence of DNA, known as the pac site, is recognized on the P22 genome. However, sequences similar to the pac site in the host genome, called pseudo-pac sites, lead to erroneous packaging and subsequent generalized transduction of Salmonella DNA. While the general genomic locations of the Salmonella pseudo-pac sites are known, the sequences themselves have not been determined. We used visualization of P22 sequencing reads mapped to host Salmonella genomes to define regions of generalized transduction initiation and the likely locations of pseudo-pac sites. We searched each genome region for the sequence with the highest similarity to the P22 pac site and aligned the resulting sequences. We built a regular expression (sequence match pattern) from the alignment and used it to search the genomes of two P22-susceptible Salmonella strains—LT2 and 14028S—for sequence matches. The final regular expression successfully identified pseudo-pac sites in both LT2 and 14028S that correspond with generalized transduction initiation sites in mapped read coverages. The pseudo-pac site sequences identified in this study can be used to predict locations of generalized transduction in other P22-susceptible hosts or to initiate generalized transduction at specific locations in P22-susceptible hosts with genetic engineering. Furthermore, the bioinformatics approach used to identify the Salmonella pseudo-pac sites in this study could be applied to other phage—host systems.}, number={6}, journal={PLOS PATHOGENS}, author={Maier, Jessie L. and Gin, Craig and Callahan, Benjamin and Sheriff, Emma K. and Duerkop, Breck A. and Kleiner, Manuel}, editor={Secor, PatrickEditor}, year={2024}, month={Jun} }
 @article{gin_petzold_uthappa_neighbors_borough_gin_lashnits_sempowski_denny_bienzle_et al._2023, title={Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020}, volume={11}, ISSN={["2167-8359"]}, DOI={10.7717/peerj.16310}, abstractNote={We collected oral and/or rectal swabs and serum from dogs and cats living in homes with SARS-CoV-2-PCR-positive persons for SARS-CoV-2 PCR and serology testing. Pre-COVID-19 serum samples from dogs and cats were used as negative controls, and samples were tested in duplicate at different timepoints. Raw ELISA results scrutinized relative to known negative samples suggested that cut-offs for IgG seropositivity may require adjustment relative to previously proposed values, while proposed cut-offs for IgM require more extensive validation. A small number of pet dogs (2/43, 4.7%) and one cat (1/21, 4.8%) were positive for SARS-CoV-2 RNA, and 28.6 and 37.5% of cats and dogs were positive for anti-SARS-CoV-2 IgG, respectively.}, journal={PEERJ}, author={Gin, Taylor E. and Petzold, Elizabeth A. and Uthappa, Diya M. and Neighbors, Coralei E. and Borough, Anna R. and Gin, Craig and Lashnits, Erin and Sempowski, Gregory D. and Denny, Thomas and Bienzle, Dorothee and et al.}, year={2023}, month={Oct} }
 @article{gin_shea_brunton_kutz_2021, title={DeepGreen: deep learning of Green's functions for nonlinear boundary value problems}, volume={11}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-021-00773-x}, abstractNote={Abstract}, number={1}, journal={SCIENTIFIC REPORTS}, author={Gin, Craig R. and Shea, Daniel E. and Brunton, Steven L. and Kutz, J. Nathan}, year={2021}, month={Nov} }
 @article{gin_daripa_2021, title={Time-dependent injection strategies for multilayer Hele-Shaw and porous media flows}, volume={6}, ISSN={["2469-990X"]}, DOI={10.1103/PhysRevFluids.6.033901}, abstractNote={Universality in the behavior of multilayer radial Hele-Shaw flows is discovered by semi-analytical methods. In particular, it is found numerically that the maximum injection rate for a stable flow decreases proportional to ${t}^{\ensuremath{-}1/3}$ regardless of the number of interfaces and increases at a rate proportional to the number of interfaces to the two-thirds power at large time $t\ensuremath{\gg}1$. However, at earlier times the number of interfaces can increase the maximum stable injection rate by a much greater amount.}, number={3}, journal={PHYSICAL REVIEW FLUIDS}, author={Gin, Craig and Daripa, Prabir}, year={2021}, month={Mar} }