Cecilia Renee Schaaf

College of Veterinary Medicine

Works (7)

Updated: June 1st, 2024 05:01

2023 journal article

A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease

FASEB JOURNAL, 37(6).

By: C. Schaaf n, K. Polkoff n, A. Carter n, A. Stewart n , B. Sheahan n, J. Freund n, J. Ginzel *, J. Snyder * ...

author keywords: APC mutation; colorectal cancer; intestine; Lgr5; porcine; stem cell; transgenic large animal
MeSH headings : Humans; Swine; Animals; Mice; In Situ Hybridization, Fluorescence; Intestines; Stem Cells; Ileum; Colon; Coleoptera; Green Fluorescent Proteins / genetics; Receptors, G-Protein-Coupled / genetics
TL;DR: This study validates ISC isolation in a new porcine Leucine Rich Repeat Containing G Protein‐Coupled Receptor 5 (LGR5) reporter line and demonstrates the use of these pigs as a novel colorectal cancer model. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2023 journal article

Changes in equine intestinal stem/progenitor cell number at resection margins in cases of small intestinal strangulation

Equine Veterinary Journal, 55(6), 995–1002.

By: B. Veerasammy  n, G. Gonzalez n, P. Báez‐Ramos n, C. Schaaf n, A. Stewart n , E. Ludwig n, C. McKinney‐Aguirre, J. Freund n, J. Robertson n , L. Gonzalez n 

Contributors: B. Veerasammy  n, G. Gonzalez n, P. Baez-Ramos n, C. Schaaf n, A. Stewart n , E. Ludwig n, C. McKinney-Aguirre , J. Freund n, J. Robertson n , L. Gonzalez n 

author keywords: horse; immunofluorescence; intestine; Ki67; small intestinal strangulation; stem cell
TL;DR: Proliferating cell and ISC numbers may be associated with case outcome, and associations between biomarker expression and the number of preoperative risk factors and postoperative complications negatively related to outcome were determined. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: January 31, 2023

2023 journal article

Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability

American Journal of Transplantation, 24(4), 564–576.

By: E. Ludwig n, N. Abraham *, C. Schaaf n, C. McKinney  n, J. Freund n, A. Stewart n , B. Veerasammy  n, M. Thomas  n ...

Contributors: E. Ludwig n, N. Abraham *, C. Schaaf n, C. McKinney  n, J. Freund n, A. Stewart n , B. Veerasammy  n, M. Thomas  n ...

author keywords: intestinal transplantation; normothermic machine perfusion; cold storage; ischemia-reperfusion injury; intestinal stem cells; preservation injury
TL;DR: Normothermic machine perfusion preservation of kidney and liver allografts minimizes CS-associated ischemia-reperfusion injury and appears to reduce IRI and improve graft regeneration with improved ISC viability and proliferation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: December 28, 2023

2022 journal article

Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications

BMC VETERINARY RESEARCH, 18(1).

By: A. Stewart n , C. Schaaf n, B. Veerasammy  n, J. Freund n & L. Gonzalez n 

author keywords: Horse; Stem cell; 3D culture; Organoid; Enteroid; Intestine; Storage
MeSH headings : Animals; Cryopreservation / veterinary; Epithelial Cells / cytology; Horses; Intestines / cytology; Stem Cells / cytology; Time Factors; Cells, Cultured; Intestinal Diseases / therapy; Intestinal Diseases / veterinary
TL;DR: Equine ISCs can be isolated and cultured after prolonged tissue storage and this suggests that ISCs could be isolated for several days from samples properly stored after procedures, including surgery or necropsy, and used to create ISC repositories for study or therapy of equine intestinal diseases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023

2022 journal article

Orthotopic Transplantation of the Full-length Porcine Intestine After Normothermic Machine Perfusion

Transplantation Direct, 8(11), e1390.

By: N. Abraham *, E. Ludwig n, C. Schaaf n, B. Veerasammy  n, A. Stewart n , C. McKinney  n, J. Freund n, J. Brassil ...

Contributors: N. Abraham *, E. Ludwig n, C. Schaaf n, B. Veerasammy  n, A. Stewart n , C. McKinney  n, J. Freund n, J. Brassil ...

TL;DR: Development and optimization of machine perfusion preservation of small intestine and successful transplantation of intestinal allografts in a porcine model are reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: October 31, 2022

2022 review

Use of Translational, Genetically Modified Porcine Models to Ultimately Improve Intestinal Disease Treatment

[Review of ]. FRONTIERS IN VETERINARY SCIENCE, 9.

By: C. Schaaf n & L. Gonzalez n 

author keywords: cystic fibrosis; colorectal cancer; ischemia-reperfusion injury; genetically altered models; intestinal disease; translational porcine model; stem cell reporter model
TL;DR: Recent advances and increased availability of gene editing technologies has led to both novel use of large animal models in clinically relevant intestinal disease research and improved testing of potential therapeutics for these diseases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 13, 2022

2021 journal article

HOPX+ injury-resistant intestinal stem cells drive epithelial recovery after severe intestinal ischemia

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 321(5), G588–G602.

By: A. Stewart n , C. Schaaf n, J. Luff n, J. Freund n, T. Becker *, S. Tufts n, J. Robertson n , L. Gonzalez n 

author keywords: epithelial repair; HOPX; ischemia; large animal models; stem cell
MeSH headings : Animals; Cell Proliferation; Disease Models, Animal; Epithelial Cells / metabolism; Epithelial Cells / pathology; Female; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Intestinal Mucosa / metabolism; Intestinal Mucosa / pathology; Male; Mesenteric Ischemia / genetics; Mesenteric Ischemia / metabolism; Mesenteric Ischemia / pathology; Phenotype; Re-Epithelialization; Severity of Illness Index; Stem Cells / metabolism; Stem Cells / pathology; Sus scrofa; Tissue Culture Techniques
TL;DR: It is demonstrated that during early in vivo recovery, injury-resistant HOPX+cells maintain quiescence, suggesting that HopX may serve a functional role in ISC mediated regeneration after injury and could be a target to control ISC proliferation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 3, 2021

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