@article{stuber_hancock_2008, title={Sustaining plant breeding-national workshop}, volume={48}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2007.07.0406spp}, abstractNote={Sustaining plant breeding was the central theme of a national workshop held in Raleigh, NC, February 8–9, 2007. The workshop was spearheaded by Ann Marie Thro, National Program Staff of USDA‐CSREES, and was co‐hosted by the Departments of Crop Science and Horticultural Science of North Carolina State University. The major catalyst for the meeting was the growing imbalance between the importance of plant breeding to the nation's future versus the steady decline in the national plant breeding investment over the past 20 years. This has led to a significant reduction in the number of public plant breeders in the U.S. and an associated substantial weakening of university education programs in this area (Frey, 1996; Guner and Wehner, 2003; Morris et al., 2006; Price, 1999). Several previous efforts have drawn attention to our nation's declining plant breeding capacity (National Plant Breeding Study, 1994). More recently, a plant breeding workshop held in 2005 at Michigan State University also focused on the decline in numbers of plant breeders in the public arena (Hancock, 2006). However, the message from these efforts was not nationally audible or sustained through the establishment of a dedicated group interested in maintaining plant breeding as a science and profession.}, number={1}, journal={CROP SCIENCE}, author={Stuber, Charles W. and Hancock, Jim}, year={2008}, pages={25–29} } @article{hancock_stuber_2008, title={Sustaining public plant breeding to meet future national needs}, volume={43}, number={2}, journal={HortScience}, author={Hancock, J. F. and Stuber, C.}, year={2008}, pages={298–299} } @article{szalma_hostert_ledeaux_stuber_holland_2007, title={QTL mapping with near-isogenic lines in maize}, volume={114}, ISSN={["1432-2242"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-34247847700&partnerID=MN8TOARS}, DOI={10.1007/s00122-007-0512-6}, abstractNote={A set of 89 near-isogenic lines (NILs) of maize was created using marker-assisted selection. Nineteen genomic regions, identified by restriction fragment length polymorphism loci and chosen to represent portions of all ten maize chromosomes, were introgressed by backcrossing three generations from donor line Tx303 into the B73 genetic background. NILs were genotyped at an additional 128 simple sequence repeat loci to estimate the size of introgressions and the amount of background introgression. Tx303 introgressions ranged in size from 10 to 150 cM, with an average of 60 cM. Across all NILs, 89% of the Tx303 genome is represented in targeted and background introgressions. The average proportion of background introgression was 2.5% (range 0-15%), significantly lower than the expected value of 9.4% for third backcross generation lines developed without marker-assisted selection. The NILs were grown in replicated field evaluations in two years to map QTLs for flowering time traits. A parallel experiment of testcrosses of each NIL to the unrelated inbred, Mo17, was conducted in the same environments to map QTLs in NIL testcross hybrids. QTLs affecting days to anthesis, days to silking, and anthesis-silk interval were detected in both inbreds and hybrids in both environments. The testing environments differed dramatically for drought stress, and different sets of QTLs were detected across environments. Furthermore, QTLs detected in inbreds were typically different from QTLs detected in hybrids, demonstrating the genetic complexity of flowering time. NILs can serve as a valuable genetic mapping resource for maize breeders and geneticists.}, number={7}, journal={THEORETICAL AND APPLIED GENETICS}, author={Szalma, S. J. and Hostert, B. M. and LeDeaux, J. R. and Stuber, C. W. and Holland, J. B.}, year={2007}, month={May}, pages={1211–1228} } @article{sanchez_goodman_stuber_2007, title={Racial diversity of maize in Brazil and adjacent areas}, volume={52}, number={1}, journal={Maydica}, author={Sanchez, J. J. and Goodman, M. M. and Stuber, C. W.}, year={2007}, pages={13–30} } @article{sanchez_goodman_bird_stuber_2006, title={Isozyme and morphological variation in maize of five Andean countries}, volume={51}, number={1}, journal={Maydica}, author={Sanchez, J. J. and Goodman, M. M. and Bird, R. M. and Stuber, C. W.}, year={2006}, pages={25–42} } @article{ledeaux_graham_stuber_2006, title={Stability of QTLs involved in heterosis in maize when mapped under several stress conditions}, volume={51}, number={1}, journal={Maydica}, author={Ledeaux, J. R. and Graham, G. I. and Stuber, C. W.}, year={2006}, pages={151–167} } @article{sanchez_goodman_stuber_2000, title={Isozymatic and morphological diversity in the races of maize of Mexico}, volume={54}, ISSN={["1874-9364"]}, DOI={10.1007/BF02866599}, number={1}, journal={ECONOMIC BOTANY}, author={Sanchez, JJ and Goodman, MM and Stuber, CW}, year={2000}, pages={43–59} } @article{sanchez_stuber_goodman_2000, title={Isozymatic diversity in the races of maize of the Americas}, volume={45}, number={3}, journal={Maydica}, author={Sanchez, J. J. and Stuber, C. W. and Goodman, M. M.}, year={2000}, pages={185–203} } @article{kaeppler_parke_mueller_senior_stuber_tracy_2000, title={Variation among maize inbred lines and detection of quantitative trait loci for growth at low phosphorus and responsiveness to arbuscular mycorrhizal fungi}, volume={40}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2000.402358x}, abstractNote={Maize (Zea mays L.) growth at low soil P levels is affected both by inherent physiological factors as well as interactions with soil microbes. The objectives of this study were (i) to quantify differences among maize inbred lines for growth at low P and response to mycorrhizal fungi, and (ii) to identify quantitative trait loci (QTL) controlling these traits in a B73 × Mo17 recombinant inbred population. Shoot dry weight and root volume were measured in the greenhouse after 6 wk of growth in a factorial experiment of 28 inbred maize lines using treatments of low vs. high P and mycorrhizal vs. nonmycorrhizal treatments. Shoot dry weight for the low P treatment in the absence of mycorrhizae ranged from 0.56 to 3.15 g. Mycorrhizal responsiveness based on shoot dry weight ranged from 106 to 800%. Shoot dry weight in the low P–nonmycorrhizal treatment was highly negatively correlated with mycorrhizal responsiveness. Plants grown at high P in the presence of mycorrhizae accumulated only 88% of the biomass of plants grown at high P in the absence of mycorrhizae, indicating that mycorrhizae can reduce plant growth when not contributing to the symbiosis. Percentage of root colonization was not correlated with mycorrhizal responsiveness. B73 and Mo17 were among the extremes for growth at low P and mycorrhizal responsiveness, and a B73 × Mo17 population of 197 recombinant inbred lines was used to detect QTL for growth at low P and mycorrhizal responsiveness. Three QTL were identified which controlled growth at low P in the absence of mycorrhizae based on shoot weight and one QTL which controlled mycorrhizal responsiveness. This study indicates that there is substantial variation among maize lines for growth at low P and response to mycorrhizal fungi. This variation could be harnessed to develop cultivars for regions of the world with P deficiency and for reduced‐input production systems.}, number={2}, journal={CROP SCIENCE}, author={Kaeppler, SM and Parke, JL and Mueller, SM and Senior, L and Stuber, C and Tracy, WF}, year={2000}, pages={358–364} } @article{marcon_kaeppler_jensen_senior_stuber_1999, title={Loci controlling resistance to high plains virus and wheat streak mosaic virus in a B73 x Mo17 population of maize}, volume={39}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci1999.0011183X003900040037x}, abstractNote={High Plains disease has the potential to cause significant yield loss in susceptible corn (Zea mays L.) and wheat (Triticum aestivum L.) genotypes, especially in the central and western USA. The primary causal agent, High Plains virus (HPV), is vectored by wheat curl mite (WCM; Aceria tossicheila Keifer), which is also the vector of wheat streak mosaic virus (WSMV). In general, the two diseases occur together as a mixed infection in the field. The objective of this research was to characterize the inheritance of HPV and WSMV resistance using B73 (resistant to HPV and WSMV) × Mo17 (moderately susceptible to HPV and WSMV) recombinant inbred lines. A population of 129 recombinant inbred lines scored for 167 molecular markers was used to evaluate resistance to WSMV and to a mixed infection of WSMV and HPV. Loci conferring resistance to systemic movement of WSMV in plants mapped to chromosomes 3, 6, and 10, consistent with the map position of wsm2, wsm1, and wsm3, respectively. Major genes for resistance to systemic spread of HPV in doubly infected plants mapped to chromosomes 3 and 6, coincident or tightly linked with the WSMV resistance loci. Analysis of doubly infected plants revealed that chromosome 6 had a major effect on HPV resistance, consistent with our previous analysis of B73 × W64A and B73 × Wf9 populations. Quantitative trait loci (QTL) affecting resistance to localized symptom development mapped to chromosomes 4 (umc66), 5 (bnl5.40), and 6 (umc85), and accounted for 24% of the phenotypic variation. Localized symptoms may reflect the amount of mite feeding or the extent of virus spread at the point of infection. Identification of cosegregating markers may facilitate selection for HPV and WSMV resistance in corn breeding programs.}, number={4}, journal={CROP SCIENCE}, author={Marcon, A and Kaeppler, SM and Jensen, SG and Senior, L and Stuber, C}, year={1999}, pages={1171–1177} } @article{stuber_polacco_lynn_1999, title={Synergy of empirical breeding, marker-assisted selection, and genomics to increase crop yield potential}, volume={39}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci1999.3961571x}, abstractNote={ABSTRACTThis paper was presented as part of the symposium entitled “Post‐Green Revolution Trends in Crop Yield Potential: Increasing, Stagnant or Greater Resistance to Stress.” In this presentation, we have focused on (i) uses of marker technology in determining the genetic basis of phenotypic expression and the manipulation of phenotypic variation in plants. This included the use of markers in understanding heterosis, in attempts to improve hybrid predictions, in quantitative trait locus (QTL) identification and mapping, in marker‐assisted selection (MAS), and in enhancing breeding success in the development of improved lines and hybrids; (ii) the role of genomics in developing a precise understanding of the genetic basis of phenotypic expression which will then provide more precision in the manipulation of phenotypic variation; and (iii) some attempts to integrate marker technology and genomics into empirical breeding strategies. In addition, we have focused on what has been successful as well as what has fallen short of expectations, and have suggested some of the possible reasons for the lack of success. Because of page limitations, we could not include an exhaustive review of the plant literature and have limited many of our examples to investigations in maize (Zea mays L).}, number={6}, journal={CROP SCIENCE}, author={Stuber, CW and Polacco, M and Lynn, M}, year={1999}, pages={1571–1583} } @article{vuylsteke_mank_antonise_bastiaans_senior_stuber_melchinger_lubberstedt_xia_stam_et al._1999, title={Two high-density AFLP (R) linkage maps of Zea mays L.: analysis of distribution of AFLP markers}, volume={99}, ISSN={["1432-2242"]}, DOI={10.1007/s001220051399}, number={6}, journal={THEORETICAL AND APPLIED GENETICS}, author={Vuylsteke, M and Mank, R and Antonise, R and Bastiaans, E and Senior, ML and Stuber, CW and Melchinger, AE and Lubberstedt, T and Xia, XC and Stam, P and et al.}, year={1999}, month={Oct}, pages={921–935} } @article{senior_murphy_goodman_stuber_1998, title={Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system}, volume={38}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1998.0011183X003800040034x}, abstractNote={Among maize (Zea maize L.) breeders, there is a heightened awareness of the necessity for both maintaining genetic diversity for crop improvement and improving the quality of genetic resource management. Restriction fragment length polymorphisms (RFLPs) and isozymes can serve as genetic markers for estimating divergence or diversity; however, the limited number of polymorphic isozyme loci available and the labor intensive and time consuming nature of RFLPs make their use for this purpose prohibitive. Simple sequence repeats (SSRs), when resolved using agarose gels, may be a viable and costeffective alternative to RFLPs and isozymes. Ninety‐four elite maize inbred lines, representative of the genetic diversity among lines derived from the Corn Belt Dent and Southern Dent maize races, were assayed for polymorphism at 70 SSR marker loci using agarose gels. The 365 alleles identified served as raw data for estimating genetic similarities among these lines. The patterns of genetic divergence revealed by the SSR polymorphisms were consistent with known pedigrees. A cluster analysis placed the inbred lines in nine clusters that correspond to major heterotic groups or market classes for North American maize. A unique fingerprint for each inbred line could be obtained from as few as five SSR loci. The utility of polymerase chain reaction (PCR)‐based markers such as SSRs for measuring genetic diversity, for assigning lines to heterotic groups and for genetic fingerprinting equals or exceeds that of RFLP markers, a property that may prove a valuable asset for a maize breeding program.}, number={4}, journal={CROP SCIENCE}, author={Senior, ML and Murphy, JP and Goodman, MM and Stuber, CW}, year={1998}, pages={1088–1098} } @article{graham_wolff_stuber_1997, title={Characterization of a yield quantitative trait locus on chromosome five of maize by fine mapping}, volume={37}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1997.0011183X003700050033x}, abstractNote={In an earlier study for identifying quantitative trait loci (QTLs) a maize (Zea mays L.) population generated from the cross B73 × Mo17, a major effect on grain yield and yield related traits was detected on chromosome 5. This chromosomal region has also shown significant associations with grain yield in several other studies. These findings have, thus, provided the impetus to further characterize this segment. A set of BC2S1 lines was created, each containing an intrugressed segment of Mo17 in a B73 background. A reciprocal set of lines, each with a B73 donor segment in a Mo17 background, also was created. These BC2S1 lines were genotyped by means of 16 restriction fragment length polymorphism (RFLP) and two isozyme markers that mapped to the targeted region on chromosome 5. From field data based on testcrosses of these lines, this one large region on chromosome 5 was dissected into at least two smaller QTLs. Effects at these two QTLs appear to act in a dominant manner, each showing significance in one testcross but not the other. These genetic factors are in repulsion phase linkage and their effects support the dominance theory of heterosis. One other segment in this region on chromosome 5 showed a significant association with yield, but it was not consistently expressed and may be spurious. The largest of these three segments has been mapped to a 27.5‐centimorgan (cM) interval near Amp3. if the observed results are indicative of the true complexity associated with QTLs having large effects, marker‐aided breeding involving such regions could be difficult, particularly if the marker‐aided breeding is based on early generation (backcross, F2, or F3) data, where the intricate nature of a region cannot be resolved.}, number={5}, journal={CROP SCIENCE}, author={Graham, GI and Wolff, DW and Stuber, CW}, year={1997}, pages={1601–1610} } @article{stuber_1997, title={Marker-assisted selection in maize}, volume={8}, ISSN={["1049-5398"]}, DOI={10.1080/10495399709525871}, number={1}, journal={ANIMAL BIOTECHNOLOGY}, author={Stuber, CW}, year={1997}, pages={91–97} } @article{stuber_1994, title={Heterosis in plant breeding}, volume={12}, DOI={10.1002/9780470650493.ch8}, abstractNote={In this review, a selected group of investigations undertaken throughout this century into the genetic, physiological and biochemical basis of heterosis are outlined, and results of recent investigations are discussed. Information is presented in sections considering: a historical perspective; genetic theories of heterosis; physiological and biochemical basis of heterosis; heterosis in population crosses; hybrid predictions for maize, rapeseed, small grains and other crops (groundnuts, tomatoes and rice); and genetics and enhancement of heterosis using molecular markers.}, journal={Plant Breeding Reviews}, author={Stuber, C. W.}, year={1994}, pages={227} } @article{stuber_lincoln_wolff_helentjaris_lander_1992, title={Identification of genetic-factors contributing to heterosis in a hybrid from 2 elite maize inbred lines using molecular markers}, volume={132}, number={3}, journal={Genetics}, author={Stuber, C. W. and Lincoln, S. E. and Wolff, D. W. and Helentjaris, T. and Lander, E. S.}, year={1992}, pages={823–839} } @article{stuber_1989, title={Marker-based selection for quantitative traits}, volume={16}, ISBN={3489622103}, journal={Science for plant breeding : proceedings of the XII. Congress of Eucarpia, February 27-March 4, 1989, Go?ttingen, Germany F.R.}, publisher={Berlin: Paul Parey Scientific Publishers}, author={Stuber, C. W.}, year={1989}, pages={31} } @article{burr_burr_thompson_albertson_stuber_1988, title={Gene-mapping with recombinant inbreds in maize}, volume={118}, number={3}, journal={Genetics}, author={Burr, B. and Burr, F. A. and Thompson, K. H. and Albertson, M. C. and Stuber, C. W.}, year={1988}, pages={519–526} } @article{edwards_stuber_wendel_1987, title={Molecular-marker-facilitated investigations of quantitative-trait loci in maize .1. numbers, genomic distribution and types of gene-action}, volume={116}, number={1}, journal={Genetics}, author={Edwards, M. D. and Stuber, C. W. and Wendel, J. F.}, year={1987}, pages={113–125} }