@article{bartz_glassbrook_danehower_cubeta_2013, title={Modulation of the phenylacetic acid metabolic complex by quinic acid alters the disease-causing activity of Rhizoctonia solani on tomato}, volume={89}, ISSN={0031-9422}, url={http://dx.doi.org/10.1016/j.phytochem.2012.09.018}, DOI={10.1016/j.phytochem.2012.09.018}, abstractNote={The metabolic control of plant growth regulator production by the plant pathogenic fungus Rhizoctonia solani Kühn (teleomorph = Thanatephorus cucumeris (A.B. Frank) Donk) and consequences associated with the parasitic and saprobic activity of the fungus were investigated. Fourteen genetically distinct isolates of the fungus belonging to anastomosis groups (AG) AG-3, AG-4, and AG-1-IA were grown on Vogel’s minimal medium N with and without the addition of a 25 mM quinic acid (QA) source of carbon. The effect of QA on fungal biomass was determined by measuring the dry wt of mycelia produced under each growth condition. QA stimulated growth of 13 of 14 isolates of R. solani examined. The production of phenylacetic acid (PAA) and the chemically related derivatives 2-hydroxy-PAA, 3-hydroxy-PAA, 4-hydroxy-PAA, and 3-methoxy-PAA on the two different media was compared by gas chromatography coupled with mass spectrometry (GC–MS). The presence of QA in the growth medium of R. solani altered the PAA production profile, limiting the conversion of PAA to derivative forms. The effect of QA on the ability of R. solani to cause disease was examined by inoculating tomato (Solanum lycopersicum L.) plants with 11 isolates of R. solani AG-3 grown on media with and without the addition of 25 mM QA. Mean percent survival of tomato plants inoculated with R. solani was significantly higher when the fungal inoculum was generated on growth medium containing QA. The results of this study support the hypotheses that utilization of QA by R. solani leads to reduced production of the plant growth regulators belonging to the PAA metabolic complex which can suppress plant disease development.}, journal={Phytochemistry}, publisher={Elsevier BV}, author={Bartz, Faith E. and Glassbrook, Norman J. and Danehower, David A. and Cubeta, Marc A.}, year={2013}, month={May}, pages={47–52} }
 @article{bartz_glassbrook_danehower_cubeta_2012, title={Elucidating the role of the phenylacetic acid metabolic complex in the pathogenic activity of Rhizoctonia solani anastomosis group 3}, volume={104}, ISSN={["0027-5514"]}, DOI={10.3852/11-084}, abstractNote={The soil fungus Rhizoctonia solani produces phytotoxic phenylacetic acid (PAA) and hydroxy (OH-) and methoxy (MeO-) derivatives of PAA. However, limited information is available on the specific role that these compounds play in the development of Rhizoctonia disease symptoms and concentration(s) required to induce a host response. Reports that PAA inhibits the growth of R. solani conflict with the established ability of the fungus to produce and metabolize PAA. Experiments were conducted to clarify the role of the PAA metabolic complex in Rhizoctonia disease. In this study the concentration of PAA and derivatives required to induce tomato root necrosis and stem canker, in the absence of the fungus, and the concentration that inhibits mycelial growth of R. solani were determined. The effect of exogenous PAA and derivatives of PAA on tomato seedling growth also was investigated. Growth of tomato seedlings in medium containing 0.1–7.5 mM PAA and derivatives induced necrosis of up to 85% of root system. Canker development resulted from injection of tomato seedling stems with 7.5 mM PAA, 3-OH-PAA, or 3-MeO-PAA. PAA in the growth medium reduced R. solani biomass, with 50% reduction observed at 7.5 mM. PAA, and derivatives were quantified from the culture medium of 14 isolates of R. solani belonging to three distinct anastomosis groups by GC-MS. The quantities ranged from below the limit of detection to 678 nM, below the concentrations experimentally determined to be phytotoxic. Correlation analyses revealed that isolates of R. solani that produced high PAA and derivatives in vitro also caused high mortality on tomato seedlings. The results of this investigation add to the body of evidence that the PAA metabolic complex is involved in Rhizoctonia disease development but do not indicate that production of these compounds is the primary or the only determinant of pathogenicity.}, number={4}, journal={MYCOLOGIA}, author={Bartz, Faith E. and Glassbrook, Norman J. and Danehower, David A. and Cubeta, Marc A.}, year={2012}, pages={793–803} }
 @article{brooks_danehower_murphy_reberg-horton_burton_2012, title={Estimation of heritability of benzoxazinoid production in rye (Secale cereale) using gas chromatographic analysis}, volume={131}, ISSN={["1439-0523"]}, DOI={10.1111/j.1439-0523.2011.01885.x}, abstractNote={With 4 tables}, number={1}, journal={PLANT BREEDING}, publisher={Wiley}, author={Brooks, Ashley M. and Danehower, David A. and Murphy, J. Paul and Reberg-Horton, S. Chris and Burton, James D.}, year={2012}, month={Feb}, pages={104–109} }
 @article{stiff_weissinger_danehower_2011, title={Analysis of CoQ(10) in Cultivated Tobacco by a High-Performance Liquid Chromatography-Ultraviolet Method}, volume={59}, ISSN={["0021-8561"]}, DOI={10.1021/jf201130z}, abstractNote={Coenzyme Q (CoQ) is a naturally occurring lipid-soluble quinone that performs multiple functions in all living cells and has become a popular antioxidant supplement, a coadjuvant in the treatment of heart disease, and the object of study for treating neurodegenerative disorders. Although there are many tools for CoQ analysis of microbial and animal samples, there have been relatively few reports of methods for CoQ analysis of green plants. This work describes a method for the routine analysis of coenzyme Q(10) in green leaf tissue of cultivated Nicotiana tabacum (tobacco) using high-performance liquid chromatography (HPLC) with UV detection. The method was applied to the analysis of CoQ(10) in N. tabacum 'KY14' leaves at different stalk positions representing young lanceolate to senescing leaves, and it was found that CoQ(10) increased as leaf position changed down the stalk from 18.69 to 82.68 μg/g fw. The method was also used to observe CoQ(10) in N. tabacum 'NC55' and N. tabacum 'TN90LC' leaves over time, finding that CoQ(10) leaf content remained relatively stable from 3 to 6 weeks but increased in both cultivars at 8 weeks. This method will likely be useful in the analysis of CoQ(10) in the green leaves of other plant species.}, number={17}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Stiff, Michael R. and Weissinger, Arthur K. and Danehower, David A.}, year={2011}, month={Sep}, pages={9054–9058} }
 @article{vontimitta_danehower_steede_moon_lewis_2010, title={Analysis of a Nicotiana tabacum L. Genomic Region Controlling Two Leaf Surface Chemistry Traits}, volume={58}, ISSN={["1520-5118"]}, DOI={10.1021/jf903256h}, abstractNote={cis-Abienol and sucrose esters are Nicotiana tabacum leaf surface components that likely influence plant resistance to pests. Their breakdown products also contribute to flavor and aroma characteristics of certain tobacco types. Mapping of genes involved in the biosynthesis of these compounds could permit development of molecular-based tools for generating tobacco types with novel cured leaf chemistry profiles. A doubled haploid mapping population segregating for major genes (Abl and BMVSE) affecting the ability to accumulate cis-abienol and sucrose esters was generated and genotyped with a large set of microsatellite markers. The two genes were found to reside on chromosome A of the N. tabacum genome with a distance of 8.2 cM (centimorgans) between them. Seventeen microsatellite markers were also placed on this linkage group, several of which exhibited complete cosegregation with Abl and BMVSE. Results should aid breeding efforts focused on modification of this aspect of tobacco cured leaf chemistry.}, number={1}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Vontimitta, Vijay and Danehower, David A. and Steede, Tyler and Moon, Hyunsook S. and Lewis, Ramsey S.}, year={2010}, month={Jan}, pages={294–300} }
 @article{chaves_shea_danehower_2008, title={Analysis of chlorothalonil and degradation products in soil and water by GC/MS and LC/MS}, volume={71}, ISSN={["0045-6535"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-39849092250&partnerID=MN8TOARS}, DOI={10.1016/j.chemosphere.2007.11.015}, abstractNote={We present a method using gas chromatography (GC) and liquid chromatography (LC) coupled to a mass selective detector to measure concentrations of the fungicide chlorothalonil and several of its metabolites in soil and water. The methods employed solid-phase extraction using a hydrophobic polymeric phase for the isolation of analytes. In lake water, average analyte recoveries ranged from 70% to 110%, with exception of pentachloronitrobenzene that gave low recoveries (23%). The method detection limits were determined to be in the range of 1 and 0.1 μg l−1 for the LC and GC methods, respectively. In soil samples, recoveries ranged from 80% to 95% for 4-hydroxy-2,5,6-trichloroisophthalonitrile (metabolite II) and 1,3-dicarbamoyl-2,4,5,6-tetrachlorobenzene (metabolite III). Limits of detection (LOD) were 0.05 and 0.02 μg g−1, respectively. Chlorothalonil and other metabolites were analyzed by GC giving recoveries ranging from 54% to 130% with LOD of 0.001−0.005 μg g−1.}, number={4}, journal={CHEMOSPHERE}, author={Chaves, Alicia and Shea, Damian and Danehower, David}, year={2008}, month={Mar}, pages={629–638} }
 @article{tombokan_aguda_danehower_kilpatrick_carbonell_2008, title={Three-component phase behavior of the sclareol-ethyl lactate-carbon dioxide system for GAS applications}, volume={45}, ISSN={["0896-8446"]}, DOI={10.1016/j.supflu.2007.12.007}, abstractNote={This paper focuses on the extraction of sclareol from the leaves of Salvia sclarea Lamiaceae, more commonly known as Clary sage. The process involves the extraction of sclareol using a CO2-soluble GRAS solvent such as ethyl lactate, followed by GAS anti-solvent precipitation from ethyl lactate solution with carbon dioxide. The three-component phase behavior of the sclareol–ethyl lactate–CO2 system at various pressures has been determined and indicates a slight cybotactic effect. The ability of thermodynamic models to predict the complex three-component phase behavior of this system is discussed, together with the implications of the thermodynamic behavior of the system on process design.}, number={2}, journal={JOURNAL OF SUPERCRITICAL FLUIDS}, author={Tombokan, Xenia C. and Aguda, Remil M. and Danehower, David A. and Kilpatrick, Peter K. and Carbonell, Ruben G.}, year={2008}, month={Jun}, pages={146–155} }
 @article{gonzalez_danehower_daub_2007, title={Vitamer levels, stress response, enzyme activity, and gene regulation of Arabidopsis lines mutant in the pyridoxine/pyridoxamine 5 '-phosphate oxidase (PDX3) and the pyridoxal kinase (SOS4) genes involved in the vitamin B-6 salvage pathway}, volume={145}, ISSN={["1532-2548"]}, DOI={10.1104/pp.107.105189}, abstractNote={Abstract}, number={3}, journal={PLANT PHYSIOLOGY}, author={Gonzalez, Eugenia and Danehower, David and Daub, Margaret E.}, year={2007}, month={Nov}, pages={985–996} }
 @article{reberg-horton_burton_danehower_ma_monks_murphy_ranells_williamson_creamer_2005, title={Changes over time in the allelochemical content of ten cultivars of rye (Secale cereale L.)}, volume={31}, ISSN={["1573-1561"]}, DOI={10.1007/s10886-005-0983-3}, abstractNote={Published studies focused on characterizing the allelopathy-based weed suppression by rye cover crop mulch have provided varying and inconsistent estimates of weed suppression. Studies were initiated to examine several factors that could influence the weed suppressiveness of rye: kill date, cultivar, and soil fertility. Ten cultivars of rye were planted with four rates of nitrogen fertilization, and tissue from each of these treatment combinations was harvested three times during the growing season. Concentrations of a known rye allelochemical DIBOA (2,4-dihydroxy-1,4-(2H)benzoxazine-3-one) were quantified from the harvested rye tissue using high performance liquid chromatography (HPLC). Phytotoxicity observed from aqueous extracts of the harvested rye tissue correlated with the levels of DIBOA recovered in harvested tissue. The amount of DIBOA in rye tissue varied depending on harvest date and rye cultivar, but was generally lower with all cultivars when rye was harvested later in the season. However, the late maturing variety 'Wheeler' retained greater concentrations of DIBOA in comparison to other rye cultivars when harvested later in the season. The decline in DIBOA concentrations as rye matures, and the fact that many rye cultivars mature at different rates may help explain why estimates of weed suppression from allelopathic agents in rye have varied so widely in the literature.}, number={1}, journal={JOURNAL OF CHEMICAL ECOLOGY}, publisher={Springer Nature}, author={Reberg-Horton, SC and Burton, JD and Danehower, DA and Ma, GY and Monks, DW and Murphy, JP and Ranells, NN and Williamson, JD and Creamer, NG}, year={2005}, month={Jan}, pages={179–193} }
 @article{finney_danehower_burton_2005, title={Gas chromatographic method for the analysis of allelopathic natural products in rye (Secale cereale L.)}, volume={1066}, ISSN={["1873-3778"]}, DOI={10.1016/j.chroma.2005.01.050}, abstractNote={Accurate and reproducible methods for the analysis of plant allelochemicals are a requirement for the study of chemical interactions between plants. This paper describes a method for sample preparation and quantitative analysis of the allelopathic chemical content of rye (Secale cereale L.) using gas chromatography (GC). Sample preparation consists of extraction of freeze-dried rye vegetative tissue with aqueous ethanol followed by partitioning of the allelochemicals into ethyl acetate, evaporation, and derivatization using the trimethylsilylating reagent N-methyl-N-trimethylsilyltrifluoroacetamide. GC analysis of the silylated mixture was performed using flame ionization detection. This method permits analysis of all known rye allelopathic agents including 2,4-dihydroxy-1,4-benzoxazin-3-one, its corresponding glucoside, 2-benzoxazolinone, β-hydroxybutyric acid, and β-phenyllactic acid. Identities of all compounds were confirmed by GC/MS analysis.}, number={1-2}, journal={JOURNAL OF CHROMATOGRAPHY A}, author={Finney, MM and Danehower, DA and Burton, JD}, year={2005}, month={Feb}, pages={249–253} }
 @article{price_pline_cranmer_danehower_2004, title={Physiological basis for cotton tolerance to flumioxazin applied postemergence directed}, volume={52}, ISSN={["1550-2759"]}, DOI={10.1614/ws-03-038r}, abstractNote={Previous research has shown that flumioxazin, a herbicide being developed as a postemergence-directed spray (PDS) in cotton, has the potential to injure cotton less than 30 cm tall if the herbicide contacts green stem tissue by rain splash or misapplication. In response to this concern, five-leaf cotton plants with chlorophyllous stems and older cotton, 16-leaf cotton plants, with bark on the lower stem were treated with a PDS containing flumioxazin plus crop oil concentrate (COC) or nonionic surfactant (NIS). Stems of treated plants and untreated plants at the respective growth stage were cross-sectioned and then magnified and photographed using bright-field microscopy techniques. More visible injury consisting of necrosis and desiccation was evident in younger cotton. Also, there was a decrease in treated-stem diameter and an increase in visible injury with COC compared with NIS in younger cotton. The effects of plant growth stage and harvest time on absorption, translocation, and metabolism of14C-flumioxazin in cotton were also investigated. Total14C absorbed at 72 h after treatment (HAT) was 77, 76, and 94% of applied at 4-, 8-, and 12-leaf growth stages, respectively. Cotton at the 12-leaf stage absorbed more14C within 48 HAT than was absorbed by four- or eight-leaf cotton by 72 HAT. A majority (31 to 57%) of applied14C remained in the treated stem for all growth stages and harvest times. Treated cotton stems at all growth stages and harvest times contained higher concentrations (Bq g−1) of14C than any other tissues. Flumioxazin metabolites made up less than 5% of the radioactivity found in the treated stem. Because of the undetectable levels of metabolites in other tissues when flumioxazin was applied PDS, flumioxazin was foliar applied to determine whether flumioxazin transported to the leaves may have been metabolized. In foliar-treated cotton, flumioxazin metabolites in the treated leaf of four-leaf cotton totaled 4% of the recovered14C 72 HAT. Flumioxazin metabolites in the treated leaf of 12-leaf cotton totaled 35% of the recovered14C 48 HAT. These data suggest that differential absorption, translocation, and metabolism at various growth stages, as well as the development of a bark layer, are the bases for differential tolerances of cotton to flumioxazin applied PDS.}, number={1}, journal={WEED SCIENCE}, author={Price, AJ and Pline, WA and Cranmer, JR and Danehower, D}, year={2004}, pages={1–7} }
 @article{mitchell_alejos-gonzalez_gracz_danehower_daub_chilton_2003, title={Xanosporic acid, an intermediate in bacterial degradation of the fungal phototoxin cercosporin}, volume={62}, ISSN={["0031-9422"]}, DOI={10.1016/S0031-9422(02)00517-4}, abstractNote={The red fungal perylenequinone phototoxin cercosporin is oxidized by Xanthomonas campestris pv zinniae to a non-toxic, unstable green metabolite xanosporic acid, identified via its lactone as 1,12-bis(2'R-hydroxypropyl)-4,9-dihydroxy-6,7-methylenedioxy-11-methoxy-3-oxaperylen-10H-10-one-2-carboxylic acid. Xanosporolactone was isolated in approximately 2:1 ratio of M:P atropisomers.}, number={5}, journal={PHYTOCHEMISTRY}, author={Mitchell, TK and Alejos-Gonzalez, F and Gracz, HS and Danehower, DA and Daub, ME and Chilton, WS}, year={2003}, month={Mar}, pages={723–732} }
 @article{fulcher_ranney_burton_walgenbach_danehower_1998, title={Natural resistance of Malus to adult Japanese beetles}, volume={188}, number={10}, journal={American Nurseryman}, author={Fulcher, A. F. and Ranney, T. G. and Burton, J. D. and Walgenbach, J. F. and Danehower, D. A.}, year={1998}, pages={56–57} }
 @article{fulcher_ranney_burton_walgenbach_danehower_1998, title={Role of foliar phenolics in host plant resistance of Malus taxa to adult Japanese beetles}, volume={33}, number={5}, journal={HortScience}, author={Fulcher, A. F. and Ranney, T. G. and Burton, J. D. and Walgenbach, J. F. and Danehower, D. A.}, year={1998}, pages={862–865} }
 @article{fulcher_ranney_burton_walgenbach_danehower_1997, title={The role of endogenous phenolics in host plant resistance among Malus taxa to Japanese beetles}, volume={42}, number={1997}, journal={Proceedings of Southern Nurserymen's Association Research Conference Annual Report}, author={Fulcher, A. F. and Ranney, T. G. and Burton, J. D. and Walgenbach, J. F. and Danehower, D. A.}, year={1997}, pages={68–70} }
 @article{danehower_kelley_1990, title={RAPID EXTRACTION AND HIGH-SPEED LIQUID-CHROMATOGRAPHY OF NICOTIANA-TABACUM LEAF PIGMENTS}, volume={502}, ISSN={["0021-9673"]}, DOI={10.1016/s0021-9673(01)89609-6}, number={2}, journal={JOURNAL OF CHROMATOGRAPHY}, author={DANEHOWER, DA and KELLEY, WT}, year={1990}, month={Mar}, pages={431–436} }
 @article{danehower_reed_wernsman_1989, title={IDENTIFICATION OF THE CHROMOSOME CARRYING THE GENE FOR PRODUCTION OF BETA-METHYLVALERYL SUCROSE ESTERS IN NICOTIANA-TABACUM}, volume={53}, ISSN={["0002-1369"]}, DOI={10.1080/00021369.1989.10869730}, abstractNote={Journal Article Identification of the Chromosome Carrying the Gene for Production of β-Methylvaleryl Sucrose Esters in Nicotiana tabacum Get access D A Danehower, D A Danehower Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695–7620, U.S.A Author to whom correspondence should be addressed. Search for other works by this author on: Oxford Academic Google Scholar S M Reed, S M Reed Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695–7620, U.S.A Search for other works by this author on: Oxford Academic Google Scholar E A Wernsman E A Wernsman Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695–7620, U.S.A Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 53, Issue 10, 1 October 1989, Pages 2813–2815, https://doi.org/10.1080/00021369.1989.10869730 Published: 01 October 1989 Article history Received: 21 April 1989 Published: 01 October 1989}, number={10}, journal={AGRICULTURAL AND BIOLOGICAL CHEMISTRY}, author={DANEHOWER, DA and REED, SM and WERNSMAN, EA}, year={1989}, month={Oct}, pages={2813–2815} }
 @article{danehower_bordner_1984, title={CUTICULAR WAX OF EPILACHNA-VARIVESTIS}, volume={14}, ISSN={["0020-1790"]}, DOI={10.1016/0020-1790(84)90045-3}, abstractNote={Cuticular lipids of larvae, pupae and adults of the Mexican bean beetle (Epilachna varivestis) have been examined using gravimetric and thin layer densitometric techniques. The effects of rearing on different hostplants and of rearing temperature on lipid composition were studied. Total lipid varied with developmental stage as well as hosplant. The amount of lipid extracted ranged from 3.0 mg/g wet weight, in the case of larvae reared on snapbeans in the field, to 5.2 mg/g wet weight for pupae reared in limas under field conditions. Total lipid increased with increasing temperature for larvae reared under controlled climatic conditions. Thin layer densitometry was used to quantify lipid classes. Epicuticular lipids included hydrocarbons, wax esters, triacylglycerols, fatty alcohols, free fatty acids, sterols, three unidentified materials and alkaloid(s). Lipids of larval and pupal stages were composed primarily of wax esters, hydrocarbons and fatty alcohols in roughly equal proportions; free fatty acids, triacylglycerols, sterols, alkaloid(s) and two unknown materials made up of the remainder. Adult cuticular lipids consisted mainly of hydrocarbons (49–60% of total lipid).}, number={6}, journal={INSECT BIOCHEMISTRY}, author={DANEHOWER, DA and BORDNER, J}, year={1984}, pages={671–676} }