David Zaharoff

Vrabel, M. R., Fesmire, C. C., Rich, M. J., Kobrin, R. L., Sano, M. B., & Zaharoff, D. A. (2024, March). A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation. CRYOBIOLOGY, Vol. 114. https://doi.org/10.1016/j.cryobiol.2023.104844

Mantooth, S. M., Hancock, A. M., Thompson, P. M., Varghese, P. J. G., Meritet, D. M., Vrabel, M. R., … Zaharoff, D. A. (2024). Characterization of an Injectable Chitosan Hydrogel for the Tunable, Localized Delivery of Immunotherapeutics. ACS BIOMATERIALS SCIENCE & ENGINEERING, 10(2), 905–920. https://doi.org/10.1021/acsbiomaterials.3c01580

Vrabel, M. R., Schulman, J. A., Gillam, F. B., Mantooth, S. M., Nguyen, K. G., & Zaharoff, D. A. (2023). Focal Cryo-Immunotherapy with Intratumoral IL-12 Prevents Recurrence of Large Murine Tumors. CANCERS, 15(8). https://doi.org/10.3390/cancers15082210

Zhang, F., Gluck, J. M., Brown, A. C., Zaharoff, D. A., & King, M. W. (2023). Heparin Affinity-Based IL-4 Delivery to Modulate Macrophage Phenotype and Endothelial Cell Activity In Vitro. ACS APPLIED MATERIALS & INTERFACES, 15(23), 27457–27470. https://doi.org/10.1021/acsami.3c00489

Zaharoff, D., & Nguyen, K. (2022, November). IDENTIFICATION OF VASOACTIVE INTESTINAL PEPTIDE (VIP)-SPECIFIC SINGLE-CHAIN ANTIBODY FRAGMENTS (SCFVS) VIA YEAST SURFACE DISPLAY. JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 10, pp. A1424–A1425. https://doi.org/10.1136/jitc-2022-SITC2022.1371

Nguyen, K. G., Mantooth, S. M., Vrabel, M. R., & Zaharoff, D. A. (2022). Intranasal Delivery of Thermostable Subunit Vaccine for Cross-Reactive Mucosal and Systemic Antibody Responses Against SARS-CoV-2. FRONTIERS IN IMMUNOLOGY, 13. https://doi.org/10.3389/fimmu.2022.858904

Paidi, S. K., Troncoso, J. R., Harper, M. G., Liu, Z., Nguyen, K. G., Ravindranathan, S., … Barman, I. (2022). Raman spectroscopy reveals phenotype switches in breast cancer metastasis. THERANOSTICS, 12(12), 5351–5363. https://doi.org/10.7150/thno.74002

Horton, M., Su, G., Yi, L., Wang, Z., Xu, Y., Pagadala, V., … Liu, J. (2021). Construction of heparan sulfate microarray for investigating the binding of specific saccharide sequences to proteins. GLYCOBIOLOGY, 31(3), 188–199. https://doi.org/10.1093/glycob/cwaa068

Mantooth, S., Zaharoff, D., & Mantooth, S. (2021, November). INJECTABLE CHITOSAN HYDROGEL FOR LOCALIZED DELIVERY OF IMMUNE CHECKPOINT INHIBITORS. JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 9, pp. A281–A281. https://doi.org/10.1136/jitc-2021-SITC2021.259

Vrabel, M., Zaharoff, D., & Mantooth, S. (2021, November). ONCO-IMMUNOLOGICAL MECHANISMS OF FOCAL ABLATION AND LOCALIZED IL-12 IMMUNOTHERAPY. JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 9, pp. A650–A650. https://doi.org/10.1136/jitc-2021-SITC2021.620

Nguyen, K. G., Wagner, E. S., Vrabel, M. R., Mantooth, S. M., Meritet, D. M., & Zaharoff, D. A. (2021). Safety and Pharmacokinetics of Intravesical Chitosan/Interleukin-12 Immunotherapy in Murine Bladders. BLADDER CANCER, 7(4), 427–437. https://doi.org/10.3233/BLC-211542

Vrabel, M., Schulman, J., & Zaharoff, D. (2020, November). INTRATUMORAL INTERLEUKIN-12 ADMINISTERED AFTER CRYOABLATION DOES NOT IMPROVE SURVIVAL IN MULTIPLE BILATERAL MURINE MODELS. JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 8, pp. A277–A278. https://doi.org/10.1136/jitc-2020-SITC2020.0457

Smith, S. G., Griffith, B. E., & Zaharoff, D. A. (2019). Analyzing the effects of instillation volume on intravesical delivery using biphasic solute transport in a deformable geometry. MATHEMATICAL MEDICINE AND BIOLOGY-A JOURNAL OF THE IMA, 36(2), 139–156. https://doi.org/10.1093/imammb/dqy004

Kerr, R., Agrawal, S., Maity, S., Koppolu, B., Jayanthi, S., Kumar, G. S., … Kumar, T. K. S. (2019). Design of a thrombin resistant human acidic fibroblast growth factor (hFGF1) variant that exhibits enhanced cell proliferation activity. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 518(2), 191–196. https://doi.org/10.1016/j.bbrc.2019.08.029

Yao, M., Rabbani, Z. N., Sattler, T., Nguyen, K. G., Zaharoff, D. A., Walker, G., & Gamcsik, M. P. (2019). Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia. ANALYTICAL CHEMISTRY, 91(23), 15032–15039. https://doi.org/10.1021/acs.analchem.9b03709

Nguyen, K. G., Gillam, F. B., Hopkins, J. J., Jayanthi, S., Gundampati, R. K., Su, G., … Zaharoff, D. A. (2019). Molecular mechanisms of heparin-induced modulation of human interleukin 12 bioactivity. Journal of Biological Chemistry, 294(12), 4412–4424. https://doi.org/10.1074/JBC.RA118.006193

Fix, S. M., Koppolu, B. P., Novell, A., Hopkins, J., Kierski, T. M., Zaharoff, D. A., … Papadopoulou, V. (2019). ULTRASOUND-STIMULATED PHASE-CHANGE CONTRAST AGENTS FOR TRANSEPITHELIAL DELIVERY OF MACROMOLECULES, TOWARD GASTROINTESTINAL DRUG DELIVERY. ULTRASOUND IN MEDICINE AND BIOLOGY, 45(7), 1762–1776. https://doi.org/10.1016/j.ultrasmedbio.2019.02.004

Davis, J. E., Gundampati, R. K., Jayanthi, S., Anderson, J., Pickhardt, A., Koppolu, B. prasanth, … Kumar, T. K. S. (2018). Effect of extension of the heparin binding pocket on the structure, stability, and cell proliferation activity of the human acidic fibroblast growth factor. Biochemistry and Biophysics Reports, 13, 45–57. https://doi.org/10.1016/J.BBREP.2017.12.001

Davis, J. E., Alghanmi, A., Gundampati, R. K., Jayanthi, S., Fields, E., Armstrong, M., … Kumar, T. K. S. (2018). Probing the role of proline-135 on the structure, stability, and cell proliferation activity of human acidic fibroblast growth factor. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 654, 115–125. https://doi.org/10.1016/j.abb.2018.07.017

Ravindranathan, S., Nguyen, K. G., Kurtz, S. L., Frazier, H. N., Smith, S. G., Koppolu, B. P., … Zaharoff, D. A. (2018). Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines. BREAST CANCER RESEARCH, 20. https://doi.org/10.1186/s13058-018-1054-3

Smith, S. G., Baltz, J. L., Koppolu, B. P., Ravindranathan, S., Nguyen, K., & Zaharoff, D. A. (2017). Immunological mechanisms of intravesical chitosan/interleukin-12 immunotherapy against murine bladder cancer. ONCOIMMUNOLOGY, 6(1). https://doi.org/10.1080/2162402x.2016.1259050

Jayanthi, S., Koppolu, B. P., Nguyen, K. G., Smith, S. G., Felber, B. K., Kumar, T. K. S., & Zaharoff, D. A. (2017). Modulation of Interleukin-12 activity in the presence of heparin. SCIENTIFIC REPORTS, 7. https://doi.org/10.1038/s41598-017-05382-1

Zaharoff, D. A., & Jewell, C. M. (2017, October). Strategic Directions in Immunoresponsive Biomaterials in Tissue Engineering INTRODUCTION. TISSUE ENGINEERING PART A, Vol. 23, pp. 1042–1043. https://doi.org/10.1089/ten.tea.2017.0395

Koppolu, B. prasanth, Smith, S. G., Ravindranathan, S., Jayanthi, S., Suresh Kumar, T. K., & Zaharoff, D. A. (2015). Corrigendum to ‘Controlling chitosan-based encapsulation for protein and vaccine delivery’ [Biomaterials 35 (2014) 4382–4389]. Biomaterials, 73, 308. https://doi.org/10.1016/J.BIOMATERIALS.2015.09.029

Smith, S. G., Koppolu, B. prasanth, Ravindranathan, S., Kurtz, S. L., Yang, L., Katz, M. D., & Zaharoff, D. A. (2015). Intravesical chitosan/interleukin-12 immunotherapy induces tumor-specific systemic immunity against murine bladder cancer. Cancer Immunology, Immunotherapy, 64(6), 689–696. https://doi.org/10.1007/S00262-015-1672-X

Koppolu, B. prasanth, Smith, S. G., Ravindranathan, S., Jayanthi, S., Suresh Kumar, T. K., & Zaharoff, D. A. (2014). Controlling chitosan-based encapsulation for protein and vaccine delivery. Biomaterials, 35(14), 4382–4389. https://doi.org/10.1016/J.BIOMATERIALS.2014.01.078

Jayanthi, S., Koppolu, B. prasanth, Smith, S. G., Jalah, R., Bear, J., Rosati, M., … Kumar, T. K. S. (2014). Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag. Protein Expression and Purification, 102, 76–84. https://doi.org/10.1016/J.PEP.2014.07.002

Smith, S., Yang, L., Vo, J., & Zaharoff, D. (2013). 583 INTRAVESICAL IMMUNOTHERAPY WITH CHITOSAN AND INTERLEUKIN-12 INDUCES SYSTEMIC TUMOR-SPECIFIC IMMUNITY. Journal of Urology, 189(4S). https://doi.org/10.1016/J.JURO.2013.02.1979

Kim, J.-W., Galanzha, E. I., Zaharoff, D. A., Griffin, R. J., & Zharov, V. P. (2013). Nanotheranostics of Circulating Tumor Cells, Infections and Other Pathological Features in Vivo. Molecular Pharmaceutics, 10(3), 813–830. https://doi.org/10.1021/mp300577s

Yang, L., & Zaharoff, D. A. (2013). Role of chitosan co-formulation in enhancing interleukin-12 delivery and antitumor activity. Biomaterials, 34(15), 3828–3836. https://doi.org/10.1016/J.BIOMATERIALS.2013.02.031

Koppolu, B., & Zaharoff, D. A. (2013). The effect of antigen encapsulation in chitosan particles on uptake, activation and presentation by antigen presenting cells. Biomaterials, 34(9), 2359–2369. https://doi.org/10.1016/j.biomaterials.2012.11.066

Heffernan, M. J., Zaharoff, D. A., Fallon, J. K., Schlom, J., & Greiner, J. W. (2011). In vivo efficacy of a chitosan/IL-12 adjuvant system for protein-based vaccines. Biomaterials, 32(3), 926–932. https://doi.org/10.1016/j.biomaterials.2010.09.058

Rogers, C. J., Zaharoff, D. A., Hance, K. W., Perkins, S. N., Hursting, S. D., Schlom, J., & Greiner, J. W. (2008). Exercise enhances vaccine-induced antigen-specific T cell responses. Vaccine, 26(42), 5407–5415. https://doi.org/10.1016/j.vaccine.2008.07.081

Zaharoff, D. A., Rogers, C. J., Hance, K. W., Schlom, J., & Greiner, J. W. (2007). Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination. Vaccine, 25(11), 2085–2094. https://doi.org/10.1016/j.vaccine.2006.11.034

Zaharoff, D. A., Rogers, C. J., Hance, K. W., Schlom, J., & Greiner, J. W. (2007). Chitosan solution enhances the immunoadjuvant properties of GM-CSF. Vaccine, 25(52), 8673–8686. https://doi.org/10.1016/j.vaccine.2007.10.037

Henshaw, J. W., Zaharoff, D. A., Mossop, B. J., & Yuan, F. (2007). Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo. Bioelectrochemistry, 71(2), 233–242. https://doi.org/10.1016/j.bioelechem.2007.07.005

Henshaw, J. W., Zaharoff, D. A., Mossop, B. J., & Yuan, F. (2006). A single molecule detection method for understanding mechanisms of electric field-mediated interstitial transport of genes. Bioelectrochemistry, 69(2), 248–253. https://doi.org/10.1016/j.bioelechem.2006.03.006

Mossop, B. J., Barr, R. C., Henshaw, J. W., Zaharoff, D. A., & Yuan, F. (2006). Electric Fields in Tumors Exposed to External Voltage Sources: Implication for Electric Field-Mediated Drug and Gene Delivery. Annals of Biomedical Engineering, 34(10), 1564–1572. https://doi.org/10.1007/S10439-006-9151-3

Henshaw, J. W., Zaharoff, D. A., Mossop, B. J., & Yuan, F. (2006). Electric field-mediated enhancement of in vivo transport of plasmid DNA in tumor interstitium. Journal of Biomechanics, 39, S377. https://doi.org/10.1016/S0021-9290(06)84518-5

McGuire, S., Zaharoff, D., & Yuan, F. (2006). Nonlinear Dependence of Hydraulic Conductivity on Tissue Deformation During Intratumoral Infusion. Annals of Biomedical Engineering, 34(7), 1173–1181. https://doi.org/10.1007/S10439-006-9136-2

Zaharoff, D. A., & Yuan, F. (2004). Effects of pulse strength and pulse duration on in vitro DNA electromobility. Bioelectrochemistry, 62(1), 37–45. https://doi.org/10.1016/j.bioelechem.2003.10.011