Works (413)

Updated: February 3rd, 2026 05:05

2026 article

Demonstrating Voxel‐by‐Voxel (V × V) Single‐Point Calibration in Whole‐Body Zebrafish by IR‐MALDESI Quantitative MSI

Joignant, A. N., Vega, S. J., & Muddiman, D. C. (2026, January 12). Journal of Mass Spectrometry, Vol. 1.

By: A. Joignant n, S. Vega n & D. Muddiman n

MeSH headings : Animals; Zebrafish / metabolism; Calibration; Arachidonic Acid / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Ion-surface interactions and analysis
Sources: NC State University Libraries, NC State University Libraries
Added: January 14, 2026

2026 article

Integrating Remote Sensing and Metabolomics to Assess Synergistic Effects of Phosphate Deficiency, Drought, and AMF Symbiosis in Soybean

Hassan, M. A., Kibbe, R. R., Muddiman, D. C., & Chang, C. Y. Y. (2026, January 1). Physiologia Plantarum, Vol. 1.

By: M. Hassan*, R. Kibbe n, D. Muddiman n & C. Chang*

MeSH headings : Mycorrhizae / physiology; Symbiosis / physiology; Glycine max / physiology; Glycine max / microbiology; Glycine max / metabolism; Droughts; Metabolomics / methods; Phosphates / deficiency; Phosphates / metabolism; Plant Roots / physiology; Plant Roots / microbiology; Plant Roots / metabolism; Photosynthesis; Chlorophyll / metabolism; Remote Sensing Technology / methods; Plant Leaves / physiology; Stress, Physiological; Genotype
topics (OpenAlex): Mycorrhizal Fungi and Plant Interactions; Plant nutrient uptake and metabolism; Biocrusts and Microbial Ecology
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6. Clean Water and Sanitation (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: January 21, 2026

2026 article

Resolving the glycosaminoglycan signature of ischemic stroke brain using PRM-based IR-MALDESI mass spectrometry imaging

Palomino, T. V., Campbell, N., Ouyang, Y., Naik, N., Hawkridge, A. M., Segura, T., & Muddiman, D. C. (2026, January 23). Analytical and Bioanalytical Chemistry, Vol. 1.

By: T. Palomino n, N. Campbell*, Y. Ouyang*, N. Naik*, A. Hawkridge*, T. Segura*, D. Muddiman n

topics (OpenAlex): Proteoglycans and glycosaminoglycans research; Mass Spectrometry Techniques and Applications; Glycosylation and Glycoproteins Research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: January 26, 2026

2026 article

Spatially resolved lipids in a mouse brain model of globoid cell leukodystrophy via IR-MALDESI MSI and parallel reaction monitoring MSI

Hunter, S. N., Wang, M. F., Thomas, B. N., Filiano, A. J., & Muddiman, D. C. (2026, January 23). Analytical and Bioanalytical Chemistry, Vol. 1.

By: S. Hunter n, M. Wang n, B. Thomas*, A. Filiano* & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Lysosomal Storage Disorders Research; Metabolomics and Mass Spectrometry Studies
Sources: NC State University Libraries, NC State University Libraries
Added: January 26, 2026

2025 article

A Strategy to Achieve Sub‐Parts‐per‐Million Mass Measurement Accuracy of N‐Linked Glycans Using Infrared Matrix‐Assisted Laser Desorption Electrospray Ionization

Adepoju, A. A., Palomino, T. V., Sohn, A. L., & Muddiman, D. C. (2025, September 29). Journal of Mass Spectrometry, Vol. 60.

By: A. Adepoju n, T. Palomino n, A. Sohn n & D. Muddiman n

author keywords: custom calibration; IR-MALDESI; mass calibration; mass measurement accuracy; N-linked glycans
MeSH headings : Polysaccharides / chemistry; Polysaccharides / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Animals; Cattle; Calibration; Spectrometry, Mass, Electrospray Ionization / methods; Fetuins / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Glycosylation and Glycoproteins Research; Analytical Chemistry and Chromatography
Sources: Web Of Science, NC State University Libraries
Added: October 13, 2025

2025 article

Comparative proteomic analysis of skin wound healing responses to biomaterial treatments identifies key pathways which govern differential regenerative outcomes

Suarez-Arnedo, A., Caston, E. L. P., Liu, Y., Bai, H., Muddiman, D. C., & Segura, T. (2025, September 4). Biomaterials, Vol. 326.

By: A. Suarez-Arnedo, E. Caston, Y. Liu, H. Bai n, D. Muddiman n & T. Segura

author keywords: Proteomics; Gene ontology; MAP scaffold; Chirality; Regeneration; Wound healing
topics (OpenAlex): Wound Healing and Treatments; Tendon Structure and Treatment; Periodontal Regeneration and Treatments
Sources: Web Of Science, NC State University Libraries
Added: September 29, 2025

2025 article

Comparative proteomic analysis of skin wound healing responses to biomaterial treatments identifies key pathways which govern differential regenerative outcomes

Suarez-Arnedo, A., Caston, E. L. P., Liu, Y., Baia, H., Muddiman, D., & Segura, T. (2025, May 24). (Vol. 5). Vol. 5.

By: A. Suarez-Arnedo*, E. Caston*, Y. Liu*, H. Baia n, D. Muddiman n & T. Segura*

topics (OpenAlex): Wound Healing and Treatments; Tendon Structure and Treatment; Periodontal Regeneration and Treatments
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: January 18, 2026

2025 article

Comparison of Mid‐Infrared and Ultraviolet Lasers Coupled to the MALDESI Source for the Detection of Secondary Metabolites and Structural Lipids in Arabidopsis thaliana

Ashbacher, S. M., Manni, J. G., & Muddiman, D. C. (2025, February 18). Journal of Mass Spectrometry, Vol. 3.

By: S. Ashbacher n, J. Manni & D. Muddiman n

author keywords: MALDESI; mass spectrometry imaging; mid-infrared; plants; ultraviolet
MeSH headings : Arabidopsis / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers; Ultraviolet Rays; Secondary Metabolism; Lipids / analysis; Lipids / chemistry; Flavonoids / analysis; Flavonoids / chemistry; Infrared Rays; Glucosinolates / analysis; Glucosinolates / chemistry; Plant Leaves / chemistry; Galactolipids / analysis; Galactolipids / chemistry; Fatty Acids / analysis; Fatty Acids / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 28, 2025

2025 article

Demonstrating voxel-by-voxel (V × V) single-point calibration in liver tissue by IR-MALDESI quantitative MSI

Bruce, E. R., Kibbe, R. R., Opperman, L. J., & Muddiman, D. C. (2025, September 29). Analytical and Bioanalytical Chemistry, Vol. 9.

By: E. Bruce n, R. Kibbe n, L. Opperman n & D. Muddiman n

author keywords: IR-MALDESI; Quantitative mass spectrometry imaging; Single-point calibration; Quantitative sampling
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
Sources: Web Of Science, NC State University Libraries
Added: October 7, 2025

2025 article

Development and Optimization of an Electrospray Diverter for Reduced Background Signal in Ambient Ionization Sources

Eisenberg, S. M., Joignant, A. N., Trumbull, J. D., Hernandez, D., Chang-Yen, D., Wouters, E. R., … Muddiman, D. C. (2025, December 8). Journal of the American Society for Mass Spectrometry, Vol. 12.

By: S. Eisenberg n, A. Joignant n, J. Trumbull*, D. Hernandez*, D. Chang-Yen*, E. Wouters*, J. Williams*, A. Radosevich*, D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Electrohydrodynamics and Fluid Dynamics
Sources: NC State University Libraries, NC State University Libraries
Added: December 9, 2025

2025 article

Distinguishing the Metabolic Effect of Fetal and Adult Fibrinogen on Human Fibroblast Cell Culture by IR-MALDESI Mass Spectrometry Imaging

Joignant, A. N., Sheridan, A., Brown, A. C., & Muddiman, D. C. (2025, April 7). Journal of the American Society for Mass Spectrometry, Vol. 4.

By: A. Joignant n, A. Sheridan n, A. Brown n & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging; fibroblast; fibrinogen
topics (OpenAlex): Blood properties and coagulation; Erythrocyte Function and Pathophysiology; Adipokines, Inflammation, and Metabolic Diseases
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 21, 2025

2025 article

Exploring ammonium salt doping to enhance ion abundance for quantitative mass spectrometry imaging

Dunnavant, A. R., Eisenberg, S. M., & Muddiman, D. C. (2025, November 4). Analytical and Bioanalytical Chemistry.

By: A. Dunnavant n, S. Eisenberg n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
Sources: NC State University Libraries, NC State University Libraries
Added: November 22, 2025

2025 article

Feasibility of IR‐MALDESI Mass Spectrometry Imaging of PFAS

Martin, A., Joignant, A. N., Farrell, M., Planchart, A., & Muddiman, D. C. (2025, January 14). Journal of Mass Spectrometry, Vol. 60.

By: A. Martin n, A. Joignant n, M. Farrell n, A. Planchart n & D. Muddiman n

author keywords: IR-MALDESI; LC-MS/MS; mass spectrometry imaging; PFAS; zebrafish
MeSH headings : Fluorocarbons / analysis; Animals; Mice; Limit of Detection; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Liver / chemistry; Liver / metabolism; Alkanesulfonic Acids / analysis; Caprylates / analysis; Feasibility Studies; Environmental Pollutants / analysis; Tandem Mass Spectrometry / methods; Zebrafish / metabolism
topics (OpenAlex): Per- and polyfluoroalkyl substances research; Lanthanide and Transition Metal Complexes; Mass Spectrometry Techniques and Applications
Sources: Web Of Science, NC State University Libraries
Added: April 28, 2025

2025 article

Glycosaminoglycan Mass Spectrometry Imaging by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Palomino, T. V., & Muddiman, D. C. (2025, March 4). Journal of the American Society for Mass Spectrometry, Vol. 3.

By: T. Palomino n & D. Muddiman n

author keywords: Chondroitin Sulfate; Glycosaminoglycans; MassSpectrometry Imaging; IR-MALDESI
MeSH headings : Animals; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Chondroitin Sulfates / analysis; Chondroitin Sulfates / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Brain Chemistry; Glycosaminoglycans / analysis; Glycosaminoglycans / chemistry; Rats; Disaccharides / analysis; Disaccharides / chemistry; Male; Mice; Brain / diagnostic imaging; Brain / metabolism
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Proteoglycans and glycosaminoglycans research; Analytical Chemistry and Chromatography
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 17, 2025

2025 article

Mechanistic Insights into Visible-Light-Induced ATRA Reactions Powered by the Symbiotic Relationship between Cu(II)/Cu(I) Phenanthroline Complexes

Arteta, S., Deegbey, M., Durand, N., Kibbe, R., Floß, J., Barth, A. T., … Castellano, F. N. (2025, August 26). ACS Catalysis, Vol. 8.

By: S. Arteta n, M. Deegbey n, N. Durand n, R. Kibbe n, J. Floß*, A. Barth n, D. Muddiman n, E. Jakubikova n, O. Reiser*, F. Castellano n

author keywords: photochemistry; photocatalysts; charge transfer; copper phenanthrolines; ATRA reactions
topics (OpenAlex): Radical Photochemical Reactions; Oxidative Organic Chemistry Reactions; Porphyrin and Phthalocyanine Chemistry
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 26, 2025

2025 article

Mitochondrial Preparation from Microglia for Glycan Analysis

Madabhushi, M., Palomino, T. V., Patil, M. H., Muddiman, D. C., Tyrrell, D. J., & Samal, J. (2025, May 30). Journal of Visualized Experiments, Vol. 5.

By: M. Madabhushi*, T. Palomino n, M. Patil*, D. Muddiman n, D. Tyrrell* & J. Samal*

MeSH headings : Microglia / chemistry; Microglia / metabolism; Polysaccharides / analysis; Mitochondria / chemistry; Mitochondria / metabolism; Animals; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Mitochondrial Proteins / chemistry; Mitochondrial Proteins / analysis; Mitochondrial Proteins / isolation & purification; Mitochondrial Proteins / metabolism; Mice
topics (OpenAlex): Neuroinflammation and Neurodegeneration Mechanisms; Glycosylation and Glycoproteins Research; Neurological Disease Mechanisms and Treatments
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 16, 2025

2025 article

Native mass spectrometry enabled by infrared matrix-assisted laser desorption electrospray ionization for rapid measurement of protein-ligand biophysical parameters

Adepoju, A. A., Ghiladi, R. A., & Muddiman, D. C. (2025, November 14). Analytical and Bioanalytical Chemistry.

By: A. Adepoju n, R. Ghiladi n, D. Muddiman n, A. Adepoju, R. Ghiladi & D. Muddiman

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Enzyme function and inhibition; Protein Interaction Studies and Fluorescence Analysis
Sources: NC State University Libraries, NC State University Libraries
Added: November 16, 2025

2025 article

Part A: Implementing an Analyte Panel and Sampling Protocol for Quality Control in Mass Spectrometry Imaging

Mills, Q., Kibbe, R. R., Sohn, A. L., Percy, A. J., Backiel, K., & Muddiman, D. C. (2025, February 17). Rapid Communications in Mass Spectrometry, Vol. 39.

By: Q. Mills n, R. Kibbe n, A. Sohn n, A. Percy*, K. Backiel* & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging; quality control; stable isotope labeled; system suitability testing
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
Sources: Web Of Science, NC State University Libraries
Added: March 3, 2025

2025 article

Part B: SLICE‐MSI—A Machine Learning Interface for System Suitability Testing of Mass Spectrometry Imaging Platforms

Kibbe, R. R., Mills, Q., Sohn, A. L., & Muddiman, D. C. (2025, February 17). Rapid Communications in Mass Spectrometry, Vol. 39.

By: R. Kibbe n, Q. Mills n, A. Sohn n & D. Muddiman n

author keywords: graphical user interface; machine learning; mass spectrometry imaging; quality control; system suitability testing
topics (OpenAlex): Machine Learning in Materials Science; Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications
Sources: Web Of Science, NC State University Libraries
Added: March 3, 2025

2025 article

Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging

Gamcsik, M. P., Bruce, E. R., & Muddiman, D. C. (2025, October 30). Bioanalysis.

By: M. Gamcsik n, E. Bruce n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Proteomics Techniques and Applications
Sources: NC State University Libraries, NC State University Libraries
Added: November 21, 2025

2025 article

Signal enhancement of lipids and glycans using ammonium fluoride for IR-MALDESI mass spectrometry imaging

Eisenberg, S. M., Dunnavant, A. R., Palomino, T. V., Muddiman, D. C., Eisenberg, S. M., Dunnavant, A. R., … Muddiman, D. C. (2025, November 18). Analytical and Bioanalytical Chemistry.

By: S. Eisenberg n, A. Dunnavant n, T. Palomino n, D. Muddiman n, S. Eisenberg, A. Dunnavant, T. Palomino, D. Muddiman

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Proteomics Techniques and Applications
Sources: NC State University Libraries, NC State University Libraries
Added: November 20, 2025

2025 article

Temperature Programming Secondary Electrospray Ionization (TP-SESI): A Novel Approach for Mass Spectrometry Analysis of Plant Volatile Organic Compounds

Ashbacher, S. M., & Muddiman, D. C. (2025, July 30). Analytical Chemistry, Vol. 7.

By: S. Ashbacher n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Chemical Sensor Technologies; Plant biochemistry and biosynthesis
Sources: Web Of Science, NC State University Libraries
Added: August 11, 2025

2025 article

Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction

Sohn, A. L., Witherspoon, J. G., Smart, R. C., & Muddiman, D. C. (2025, February 3). Analytical and Bioanalytical Chemistry, Vol. 2.

By: A. Sohn n, J. Witherspoon n, R. Smart n & D. Muddiman n

author keywords: 3D; Mass spectrometry imaging; Lipids; Automatic <italic>z</italic>-axis correction; Top-hat
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 3, 2025

2025 article

Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging

Mills, Q., Ashbacher, S. M., Sohn, A. L., & Muddiman, D. C. (2025, March 15). Analytical and Bioanalytical Chemistry, Vol. 3.

By: Q. Mills n, S. Ashbacher n, A. Sohn n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Plant imaging; Plant metabolomics; Plant biology; <italic>Brassica oleracea</italic> var. <italic>viridis</italic>
MeSH headings : Brassica / chemistry; Plant Leaves / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 19, 2025

2025 article

Unraveling the molecular dynamics of wound healing: integrating spatially resolved lipidomics and temporally resolved proteomics

Bai, H., Arnedo, A. S., Liu, Y., Segura, T., & Muddiman, D. (2025, April 24). Analytical and Bioanalytical Chemistry, Vol. 4.

By: H. Bai n, A. Arnedo*, Y. Liu*, T. Segura* & D. Muddiman*

author keywords: Wound healing; Mass spectrometry imaging; 3D; IR-MALDESI; Multi-omics
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Forensic Entomology and Diptera Studies; Spectroscopy Techniques in Biomedical and Chemical Research
Sources: Web Of Science, NC State University Libraries
Added: May 6, 2025

2024 article

A statistical approach to system suitability testing for mass spectrometry imaging

Sohn, A. L., Kibbe, R. R., Dioli, O. E., Hector, E. C., Bai, H., Garrard, K. P., & Muddiman, D. C. (2024, March 8). Rapid Communications in Mass Spectrometry.

By: A. Sohn n, R. Kibbe n, O. Dioli n, E. Hector n, H. Bai n, K. Garrard n, D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Pesticide Residue Analysis and Safety
Sources: Web Of Science, NC State University Libraries
Added: March 18, 2024

2024 article

Absolute Quantification of Glutathione Using Top‐Hat Optics for IR‐MALDESI Mass Spectrometry Imaging

Bruce, E. R., Kibbe, R. R., Hector, E. C., & Muddiman, D. C. (2024, September 18). Journal of Mass Spectrometry, Vol. 59.

By: E. Bruce n, R. Kibbe n, E. Hector n & D. Muddiman n

author keywords: diffractive optical element; mass spectrometry imaging; qMSI; top-hat IR-MALDESI
MeSH headings : Glutathione / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Animals
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 30, 2024

2024 article

Improved detection in untargeted lipidomics through silver‐doped infrared matrix‐assisted laser desorption electrospray ionization

Eisenberg, S. M., & Muddiman, D. C. (2024, May 30). Rapid Communications in Mass Spectrometry, Vol. 38.

By: S. Eisenberg n & D. Muddiman n

MeSH headings : Animals; Mice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lipids / chemistry; Lipids / analysis; Liver / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Lipidomics / methods; Silver / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Proteomics Techniques and Applications
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 11, 2024

2024 article

In-depth characterization of N-glycosylation and sialic acid content in fetal and adult fibrinogen

Palomino, T. V., Sheridan, A., Muddiman, D. C., & Brown, A. C. (2024, November 1). Research and Practice in Thrombosis and Haemostasis, Vol. 8.

By: T. Palomino n, A. Sheridan n, D. Muddiman n & A. Brown n

author keywords: fetal; fibrinogen; glycan; mass spectrometry; neonate; sialic acid
topics (OpenAlex): Blood properties and coagulation; Glycosylation and Glycoproteins Research; Proteoglycans and glycosaminoglycans research
Sources: Web Of Science, NC State University Libraries
Added: December 16, 2024

2024 journal article

Incorporation of Three Different Optical Trains into the IR-MALDESI Mass Spectrometry Imaging Platform to Characterize Artemisia annua

Journal of the American Society for Mass Spectrometry, 35(6), 1245–1252.

By: S. Ashbacher n, Q. Mills n, A. Sohn n, D. Xie n & D. Muddiman n

Contributors: S. Ashbacher n, Q. Mills n, A. Sohn n, D. Xie n & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging; laseroptics; Artemisia annua
MeSH headings : Artemisia annua / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Artemisinins / analysis; Artemisinins / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: May 1, 2024

2024 article

Leveraging Supervised Machine Learning Algorithms for System Suitability Testing of Mass Spectrometry Imaging Platforms

Kibbe, R. R., Sohn, A. L., & Muddiman, D. C. (2024, September 3). Journal of Proteome Research, Vol. 9.

By: R. Kibbe n, A. Sohn n & D. Muddiman n

author keywords: mass spectrometry imaging; machine learning; quality control; system suitability testing; IR-MALDESI
MeSH headings : Supervised Machine Learning; Algorithms; Mass Spectrometry / methods; Quality Control; Reproducibility of Results; Humans
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Machine Learning in Materials Science
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2024

2024 article

Mass spectrometry imaging of N‐linked glycans: Fundamentals and recent advances

Palomino, T. V., & Muddiman, D. C. (2024, June 27). Mass Spectrometry Reviews, Vol. 6.

By: T. Palomino n & D. Muddiman n

author keywords: glycomics; mass spectrometry imaging; N-linked glycans
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Glycosylation and Glycoproteins Research; Advanced Proteomics Techniques and Applications
Sources: Web Of Science, NC State University Libraries
Added: July 8, 2024

2024 article

Obtaining 20 μm Spatial Resolution with a 2940 nm Laser by IR-MALDESI Mass Spectrometry Imaging

Eisenberg, S. M., Joignant, A. N., Knizner, K. T., Manni, J. G., & Muddiman, D. C. (2024, November 8). Journal of the American Society for Mass Spectrometry, Vol. 11.

By: S. Eisenberg n, A. Joignant n, K. Knizner n, J. Manni & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging; mid-IRlaser; spatial resolution
MeSH headings : Animals; Mice; Lasers; Mass Spectrometry / methods; Liver / chemistry; Liver / diagnostic imaging; Brain / diagnostic imaging; Brain / metabolism; Brain Chemistry; Lipids / analysis; Lipids / chemistry; Neurotransmitter Agents / analysis
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Spectroscopy Techniques in Biomedical and Chemical Research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 18, 2024

2024 article

Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain

Wang, M. F., Ouyang, Y., Segura, T., & Muddiman, D. C. (2024, June 1). Analytical and Bioanalytical Chemistry, Vol. 6.

By: M. Wang n, Y. Ouyang*, T. Segura* & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Sucrose; PFA; Neurotransmitter
MeSH headings : Animals; Neurotransmitter Agents / analysis; Neurotransmitter Agents / metabolism; Mice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Brain / metabolism; Mice, Inbred C57BL; Brain Chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 11, 2024

2024 article

Quantitative mass spectrometry imaging (qMSI): A tutorial

Kibbe, R. R., & Muddiman, D. C. (2024, March 15). Journal of Mass Spectrometry, Vol. 59.

By: R. Kibbe n & D. Muddiman n

author keywords: absolute quantification; mass spectrometry imaging; qMSI; relative quantification
MeSH headings : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: This tutorial will detail the main factors to consider when designing a qMSI experiment and highlight the methods that have been developed to overcome these added complexities, specifically for those newer to the field of MSI. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 25, 2024

2024 article

Quantitative sampling by IR-MALDESI is not susceptible to tissue heterogeneity in a multi-organ model

Joignant, A. N., Hector, E. C., Barnes, M. M., Kullman, S. W., & Muddiman, D. C. (2024, November 13). Analytical and Bioanalytical Chemistry, Vol. 11.

By: A. Joignant n, E. Hector n, M. Barnes n, S. Kullman n & D. Muddiman*

author keywords: IR-MALDESI; Mass spectrometry imaging; Quantitative; Zebrafish
MeSH headings : Animals; Zebrafish; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Liver / chemistry
topics (OpenAlex): Spectroscopy Techniques in Biomedical and Chemical Research; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
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2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 25, 2024

2024 article

RCM protocols: improving reproducibility in the field of mass spectrometry

Holman, S. W., Langley, G. J., Muddiman, D. C., & Novak‐Mitchell, C. (2024, January 1). Rapid Communications in Mass Spectrometry, Vol. 1.

By: S. Holman*, G. Langley*, D. Muddiman n & C. Novak‐Mitchell*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Isotope Analysis in Ecology; Analytical chemistry methods development
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Sources: Web Of Science, NC State University Libraries
Added: January 16, 2024

2024 article

Rigorous scientific inquiry guided by creativity, curiosity and support: One of the last Renaissance scientists of our time

Muddiman, D. C., & Hittle, L. R. (2024, July 9). Analytical and Bioanalytical Chemistry, Vol. 7.

By: D. Muddiman n & L. Hittle*

MeSH headings : Creativity; Humans; Exploratory Behavior; Science; History, 20th Century
topics (OpenAlex): Science and Climate Studies; Ophthalmology and Visual Health Research
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2024

2024 article

Turning the Tide Toward Equity: Gender Disparities in Scientific Publishing in Biomedical Mass Spectrometry

Sahoo, A., Chie, C., Muddiman, D. C., & Samal, J. (2024, September 25). GEN Biotechnology, Vol. 9.

By: A. Sahoo, C. Chie, D. Muddiman n & J. Samal*

topics (OpenAlex): Diversity and Career in Medicine; scientometrics and bibliometrics research; Health and Medical Research Impacts
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4. Quality Education (Web of Science)
5. Gender Equality (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 30, 2024

2023 article

Achieving Cross-Ring Fragmentation of N-Linked Glycans by IR-MALDESI

Palomino, T. V., & Muddiman, D. C. (2023, December 19). Journal of the American Society for Mass Spectrometry, Vol. 35, pp. 166–171.

By: T. Palomino n & D. Muddiman n

author keywords: N-linked glycans; lithium doping; fragmentation; IR-MALDESI; cross-ring fragment
MeSH headings : Tandem Mass Spectrometry; Lithium / chemistry; Polysaccharides / chemistry; Metals / chemistry; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 16, 2024

2023 article

Achieving Sub-Parts-per-Million Mass Measurement Accuracy on an Orbitrap Mass Spectrometry Imaging Platform without Automatic Gain Control

Kibbe, R. R., & Muddiman, D. C. (2023, April 25). Journal of the American Society for Mass Spectrometry.

By: R. Kibbe n & D. Muddiman n

author keywords: centroid; mass measurement accuracy; IR-MALDESI; mass spectrometry imaging; Orbitrap; automatic gain control
MeSH headings : Animals; Mice; Mass Spectrometry / methods; Data Accuracy; Lipids
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Isotope Analysis in Ecology
TL;DR: There is support that natively collecting centroid MS data significantly improves MMA to sub-ppm levels without AGC and reduces false positive annotations. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 15, 2023

2023 article

Automatic z-Axis Correction for IR-MALDESI Mass Spectrometry Imaging of Uneven Surfaces

Knizner, K. T., Garrard, K. P., & Muddiman, D. C. (2023, June 12). Journal of the American Society for Mass Spectrometry, Vol. 6.

By: K. Knizner n, K. Garrard n & D. Muddiman n

author keywords: mass spectrometry imaging; nonflat surfaces; IR-MALDESI; automatic z-axis correction
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
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Sources: Web Of Science, NC State University Libraries
Added: June 26, 2023

2023 article

Comparative analysis of sucrose-embedding for whole-body zebrafish MSI by IR-MALDESI

Wang, M. F., Ritter, M. M., Kullman, S. W., & Muddiman, D. C. (2023, August 29). Analytical and Bioanalytical Chemistry, Vol. 8.

By: M. Wang n, M. Ritter n, S. Kullman n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Sucrose; PFA; Zebrafish
MeSH headings : Animals; Zebrafish; Sucrose / analysis; Sucrose / metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tissue Embedding / methods; Lipids / analysis; Lipids / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Physiological and biochemical adaptations
TL;DR: Danio rerio, or zebrafish, are model organisms for various disease states and can be imaged as a multi-organ sample to analyze morphological and metabolomic preservation as a result of sample preparation to compare sucrose-embedding with paraformaldehyde fixation against conventional fresh-frozen sample preparation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 25, 2023

2023 article

Correction to “On the importance of color in mass spectrometry imaging”

Muddiman, D. C. (2023, April 26). Correction to “On the importance of color in mass spectrometry imaging.” Journal of Mass Spectrometry, Vol. 58.

By: D. Muddiman n

topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Advanced Chemical Sensor Technologies
Sources: Web Of Science, NC State University Libraries
Added: May 15, 2023

2023 article

Correction to “Time of acquisition and high spatial resolution mass spectrometry imaging”

Muddiman, D. C. (2023, April 26). Correction to “Time of acquisition and high spatial resolution mass spectrometry imaging.” Journal of Mass Spectrometry.

By: D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Isotope Analysis in Ecology
Sources: Web Of Science, NC State University Libraries
Added: May 15, 2023

2023 article

Development of an object-based image analysis tool for mass spectrometry imaging ion classification

Eisenberg, S. M., Knizner, K. T., & Muddiman, D. C. (2023, May 24). Analytical and Bioanalytical Chemistry, Vol. 5.

By: S. Eisenberg n, K. Knizner n & D. Muddiman n

author keywords: IR-MALDESI; Object-based image analysis; Mass spectrometry imaging; Image segmentation; Image classification; MATLAB
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Chemical Sensor Technologies
TL;DR: The utility of an ion classification tool (ICT) developed using object-based image analysis in MATLAB is presented, which was able to accurately classify 45/50 ions as on-tissue or background. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 3, 2023

2023 article

Enhanced Detection of Charged N-Glycans in the Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometric Imaging

Samal, J., Palomino, T. V., Chen, J., Muddiman, D. C., & Segura, T. (2023, July 10). Analytical Chemistry, Vol. 95, pp. 10913–10920.

By: J. Samal*, T. Palomino n, J. Chen*, D. Muddiman n & T. Segura*

topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Carbohydrate Chemistry and Synthesis
TL;DR: This work demonstrates the first application of IR-MALDESI in N-linked glycan imaging of the brain tissue, leading to a 2.5-fold increase in the in situ total brain N-glycan detection compared to the current gold standard of positive-mode matrix-assisted laser desorption/ionization mass spectrometry imaging. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 31, 2023

2023 article

Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix‐assisted laser desorption electrospray ionization

Joignant, A. N., Knizner, K. T., & Muddiman, D. C. (2023, September 18). Rapid Communications in Mass Spectrometry, Vol. 37.

By: A. Joignant n, K. Knizner n & D. Muddiman n

MeSH headings : Mice; Animals; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Histological Techniques; Light; Lasers
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: This work defines an optimal sample thickness for IR-MALDESI-MSI and demonstrates the utility of optimizing tissue thickness for MSI platforms of comparable Z resolution. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

2023 article

Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non‐flat samples

Joignant, A. N., & Muddiman, D. C. (2023, February 23). Journal of Mass Spectrometry, Vol. 3.

By: A. Joignant n & D. Muddiman n

author keywords: depth of focus; laser wavelength; mass spectrometry imaging; spatial resolution; surface roughness
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Spectroscopy and Laser Applications; Spectroscopy Techniques in Biomedical and Chemical Research
Sources: Web Of Science, NC State University Libraries
Added: April 4, 2023

2023 article

Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI

Wang, M. F., Sohn, A. L., Samal, J., Erning, K., Segura, T., & Muddiman, D. C. (2023, March 29). Journal of the American Society for Mass Spectrometry, Vol. 3.

By: M. Wang n, A. Sohn n, J. Samal*, K. Erning*, T. Segura* & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging; sucrose; PFA
MeSH headings : Mice; Animals; Spectrometry, Mass, Electrospray Ionization / methods; Lipidomics; Ice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ions / chemistry; Lipids / analysis; Brain
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: Evidence from these investigations supports sucrose-embedding without ice matrix as an alternative preparatory technique for IR-MALDESI-MSI, and demonstrates improved spatial distribution of ions resulting from the cryo-protective property of sucrose and paraformaldehyde fixation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: April 24, 2023

2023 article

Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs)

Joignant, A. N., Ritter, M. M., Knizner, K. T., Garrard, K. P., Kullman, S. W., & Muddiman, D. C. (2023, August 1). Journal of the American Society for Mass Spectrometry, Vol. 8.

By: A. Joignant n, M. Ritter n, K. Knizner n, K. Garrard n, S. Kullman n & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging; spatialresolution; nested region of interest; zebrafish
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Physiological and biochemical adaptations
TL;DR: The nROI method was characterized on mouse liver and applied to top-hat MSI of zebrafish using a novel optical train, which resulted in a significant improvement in both acquisition time and spatial detail of the zebra fish. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 21, 2023

2023 article

Metabolite Annotation Confidence Score (MACS): A Novel MSI Identification Scoring Tool

Eisenberg, S. M., Knizner, K. T., & Muddiman, D. C. (2023, August 22). Journal of the American Society for Mass Spectrometry, Vol. 8.

By: S. Eisenberg n, K. Knizner n & D. Muddiman n

author keywords: IR-MALDESI; annotation scoring; mass measurementaccuracy; spectral accuracy; SSIM; MATLAB
MeSH headings : Mass Spectrometry; Databases, Factual; Ions
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Computational Drug Discovery Methods; Mass Spectrometry Techniques and Applications
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Sources: Web Of Science, NC State University Libraries
Added: September 5, 2023

2023 article

Predicting Sialic Acid Content of N-Linked Glycans Using the Isotopic Pattern of Chlorine

Palomino, T. V., & Muddiman, D. C. (2023, June 8). Journal of the American Society for Mass Spectrometry, Vol. 6.

By: T. Palomino n & D. Muddiman n

author keywords: N-linked glycans; sialic acid; chlorine; IR-MALDESI
MeSH headings : Humans; N-Acetylneuraminic Acid; Chlorine; Polysaccharides / chemistry; Sialic Acids / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Glycosylation and Glycoproteins Research; Analytical Chemistry and Chromatography
TL;DR: A new rule is described that can predict the number of sialic acids on a glycan and grants confident glycan annotations and compositions beyond accurate mass measurements, thereby further improving the capability of IR-MALDESI to study sialylated N-linked glycans within biological tissues. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 26, 2023

2023 article

Prototyping an ionization source for non‐engineers

Knizner, K. T., Eisenberg, S. M., & Muddiman, D. C. (2023, December 21). Journal of Mass Spectrometry, Vol. 59.

By: K. Knizner n, S. Eisenberg n & D. Muddiman n

author keywords: computer-aided design; hardware interfacing; instrumentation prototyping; microcontroller; software development
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
Sources: Web Of Science, NC State University Libraries
Added: January 2, 2024

2023 article

Quasi‐continuous infrared matrix‐assisted laser desorption electrospray ionization source coupled to a quadrupole time‐of‐flight mass spectrometer for direct analysis from well plates

Arciniega, C., Garrard, K. P., Guymon, J. P., Manni, J. G., Apffel, A., Fjeldsted, J. C., & Muddiman, D. C. (2023, January 1). Journal of Mass Spectrometry, Vol. 58.

By: C. Arciniega n, K. Garrard n, J. Guymon n, J. Manni*, A. Apffel*, J. Fjeldsted*, D. Muddiman n

author keywords: ambient ionization; direct analysis; IR-MALDESI; Q-TOF mass spectrometer
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: An infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) source coupled directly to an Agilent 6545 for direct analysis from well plates and is a proof of concept for HTS applications using IR‐MAL DESI coupled to a different MS platform. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: February 13, 2023

2023 article

SMART: A data reporting standard for mass spectrometry imaging

Sohn, A. L., Joignant, A. N., Cologna, S. M., Prentice, B. M., & Muddiman, D. C. (2023, January 22). Journal of Mass Spectrometry, Vol. 58.

By: A. Sohn n, A. Joignant n, S. Cologna*, B. Prentice* & D. Muddiman n

author keywords: data reporting standards; mass spectrometry imaging
MeSH headings : Research Design; Mass Spectrometry / methods; Ions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Proteomics Techniques and Applications
TL;DR: The goal of this tutorial is to discuss the necessity of establishing a uniform reporting system to communicate MSI data in publications and presentations in a simple format to readily interpret the parameters and baseline outcomes of the data. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: March 13, 2023

2022 article

An adaptive teosinte mexicana introgression modulates phosphatidylcholine levels and is associated with maize flowering time

Barnes, A. C., Rodríguez-Zapata, F., Juárez-Núñez, K. A., Gates, D. J., Janzen, G. M., Kur, A., … Rellán-Álvarez, R. (2022, June 30). Proceedings of the National Academy of Sciences, Vol. 7.

Contributors: P. Thompson n

author keywords: phospholipid metabolism; maize genetics; highland adaptation; flowering time; selection
MeSH headings : Adaptation, Physiological; Alleles; Chromosome Mapping; Flowers / genetics; Flowers / metabolism; Gene-Environment Interaction; Genes, Plant; Genetic Linkage; Phosphatidylcholines / metabolism; Phospholipases A1 / classification; Phospholipases A1 / genetics; Phospholipases A1 / metabolism; Plant Proteins / classification; Plant Proteins / genetics; Plant Proteins / metabolism; Zea mays / genetics; Zea mays / growth & development
topics (OpenAlex): Lipid metabolism and biosynthesis; Genetic Mapping and Diversity in Plants and Animals; Adipose Tissue and Metabolism
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UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 3, 2022

2022 article

Developing transmission mode for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging

Joignant, A. N., Bai, H., Guymon, J. P., Garrard, K. P., Pankow, M., & Muddiman, D. C. (2022, September 3). Rapid Communications in Mass Spectrometry, Vol. 11.

By: A. Joignant n, H. Bai n, J. Guymon n, K. Garrard n, M. Pankow n & D. Muddiman n

MeSH headings : Animals; Glycerol; Ice; Lasers; Mammals; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: This work has successfully demonstrated the adaptation of the NextGen IR‐MAL DESI source through the feasibility of tm‐IR‐MALDESI mass spectrometry imaging on mammalian tissue, expanding future biological applications of the method. (via Semantic Scholar)
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7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 26, 2022

2022 article

Development and validation of a high resolving power absolute quantitative per‐ and polyfluoroalkyl substances method incorporating Skyline data processing

Enders, J. R., Weed, R. A., Griffith, E. H., & Muddiman, D. C. (2022, March 11). Rapid Communications in Mass Spectrometry, Vol. 6.

By: J. Enders n, R. Weed n, E. Griffith n & D. Muddiman n

MeSH headings : Chromatography, Liquid; Fluorocarbons; Mass Spectrometry / methods; Reproducibility of Results; Software
topics (OpenAlex): Per- and polyfluoroalkyl substances research; Toxic Organic Pollutants Impact; Atmospheric chemistry and aerosols
TL;DR: This method demonstrates the feasibility of performing reproducible absolute quantitation of PFAS on an HRAM platform and does so using an open-source vendor-neutral data processing software to facilitate sharing of data across labs and institutions. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 4, 2022

2022 journal article

Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance

Cell Metabolism, 34(7), 1042–1053.e6.

By: Y. Wu, C. Wong, E. Chiles, A. Mellinger n, H. Bae*, S. Jung*, T. Peterson*, J. Wang* ...

MeSH headings : Blood Glucose; Diabetes Mellitus, Type 2; Diet, High-Fat / adverse effects; Dietary Fats / pharmacology; Fructose / metabolism; Glucose / metabolism; Glucose Intolerance / metabolism; Humans; Insulin / metabolism; Islets of Langerhans / metabolism
topics (OpenAlex): Diet, Metabolism, and Disease; Liver Disease Diagnosis and Treatment; Diet and metabolism studies
TL;DR: It is reported that dietary fat enhances intestinal fructose metabolism, which releases glycerate into the blood, which provides a link between dietary fructose and diabetes that is modulated by dietary fat. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 8, 2022

2022 article

Highlighting Functional Mass Spectrometry Imaging Methods in Bioanalysis

Mellinger, A. L., Muddiman, D. C., & Gamcsik, M. P. (2022, June 24). Journal of Proteome Research, Vol. 6.

By: A. Mellinger n, D. Muddiman n & M. Gamcsik n

author keywords: mass spectrometry imaging; kinetics; rate; isotope; metabolism; dynamics
MeSH headings : Magnetic Resonance Imaging; Mass Spectrometry / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: MSI exceeds some of the classic functional imaging methods, demonstrating the ability to provide functional data from the nanoscale (subcellular) to whole tissue or organ level, and this Perspective highlights several examples of how different MSI ionization and detection technologies can provide unprecedented detailed spatial maps of time-dependent biological processes. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 11, 2022

2022 article

Improved spatial resolution of infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective

Joignant, A. N., Bai, H., Manni, J. G., & Muddiman, D. C. (2022, September 4). Rapid Communications in Mass Spectrometry, Vol. 12.

By: A. Joignant n, H. Bai n, J. Manni & D. Muddiman n

MeSH headings : Animals; Spectrometry, Mass, Electrospray Ionization / methods; Ice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers; Mammals
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Isotope Analysis in Ecology; Metabolomics and Mass Spectrometry Studies
Sources: Web Of Science, NC State University Libraries
Added: October 17, 2022

2022 article

Interrogating the Metabolomic Profile of Amyotrophic Lateral Sclerosis in the Post-Mortem Human Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging (MSI)

Sohn, A. L., Ping, L., Glass, J. D., Seyfried, N. T., Hector, E. C., & Muddiman, D. C. (2022, November 10). Metabolites, Vol. 12.

By: A. Sohn n, L. Ping*, J. Glass*, N. Seyfried*, E. Hector n & D. Muddiman n

author keywords: IR-MALDESI MSI; mass spectrometry imaging; Amyotrophic lateral sclerosis (ALS); neurodegenerative disease; multiomic
topics (OpenAlex): Amyotrophic Lateral Sclerosis Research; Genetic Neurodegenerative Diseases; Mitochondrial Function and Pathology
TL;DR: This work is the first of its kind, reporting on the investigation of ALS post-mortem human brain tissue analyzed by multiomic MSI, and nearly 300 metabolites were identified across the sixteen samples where 25 were identified as dysregulated between disease cohorts. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 3, 2023

2022 article

Mapping glycine uptake and its metabolic conversion to glutathione in mouse mammary tumors using functional mass spectrometry imaging

Mellinger, A. L., Kibbe, R. R., Rabbani, Z. N., Meritet, D., Muddiman, D. C., & Gamcsik, M. P. (2022, November 1). Free Radical Biology and Medicine, Vol. 193, pp. 677–684.

By: A. Mellinger n, R. Kibbe n, Z. Rabbani n, D. Meritet n, D. Muddiman n & M. Gamcsik n

MeSH headings : Mice; Animals; Glycine; Biological Transport; Glutathione; Mammary Neoplasms, Animal; Mass Spectrometry
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Sulfur Compounds in Biology
TL;DR: A novel functional mass spectrometry imaging (fMSI) method that can map the variations in the conversion of glycine to glutathione metabolic activity across tumor tissue sections by tracking the fate of three glycine isotopologues administered in a timed sequence to tumor-bearing anesthetized mice is developed. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 23, 2023

2022 article

Next-Generation Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Source for Mass Spectrometry Imaging and High-Throughput Screening

Knizner, K. T., Guymon, J. P., Garrard, K. P., Bouvrée, G., Manni, J., Hauschild, J.-P., … Muddiman, D. C. (2022, September 29). Journal of the American Society for Mass Spectrometry, Vol. 9.

By: K. Knizner n, J. Guymon n, K. Garrard n, G. Bouvrée, J. Manni, J. Hauschild*, K. Strupat*, K. Fort* ...

author keywords: IR-MALDESI; High-Throughput Screening; Mass Spectrometry Imaging; Orbitrap Mass Spectrometer; Design of Experiments
MeSH headings : High-Throughput Screening Assays; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
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Sources: Web Of Science, NC State University Libraries
Added: October 17, 2022

2022 article

Normalization techniques for high‐throughput screening by infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry

Knizner, K. T., Bagley, M. C., Pu, F., Elsen, N. L., Williams, J. D., & Muddiman, D. C. (2022, May 28). Journal of Mass Spectrometry, Vol. 6.

By: K. Knizner n, M. Bagley n, F. Pu*, N. Elsen*, J. Williams* & D. Muddiman n

author keywords: high-throughput screening; IR-MALDESI; normalization; Orbitrap mass spectrometer
MeSH headings : Drug Discovery; High-Throughput Screening Assays; Lasers; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: Optimal solvent compositions for increasing measured analyte abundances with direct analysis by IR‐MALDESI‐MS are reported and normalization strategies, such as adding a normalization standard that is similar or dissimilar in structure to the model target drug, are evaluated. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 20, 2022

2022 article

Novel matrix strategies for improved ionization and spatial resolution using IR‐MALDESI mass spectrometry imaging

Kibbe, R. R., Mellinger, A. L., & Muddiman, D. C. (2022, July 19). Journal of Mass Spectrometry, Vol. 8.

By: R. Kibbe n, A. Mellinger n & D. Muddiman n

author keywords: energy-absorbing matrix; glycerol; IR-MALDESI; mass spectrometry imaging
MeSH headings : Animals; Glycerol; Ice; Lipids; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: It was observed that utilizing a 50% glycerol matrix without ice provides improved spatial resolution with lipid abundances and annotations comparable to the ice matrix standard, while decreasing the time required to complete an IR‐MALDESI tissue imaging experiment. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 8, 2022

2022 article

On the importance of color in mass spectrometry imaging

Knizner, K. T., Kibbe, R. R., Garrard, K. P., Nuñez, J. R., Anderton, C. R., & Muddiman, D. C. (2022, November 22). Journal of Mass Spectrometry, Vol. 57.

author keywords: colormap; CVD; data visualization; MSI
MeSH headings : Humans; Mass Spectrometry
topics (OpenAlex): Data Visualization and Analytics; Species Distribution and Climate Change; Mass Spectrometry Techniques and Applications
TL;DR: Best practices for choosing a colormap to accurately display MSI data, improve readability, and accommodate all CVDs are presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 9, 2023

2022 article

Optimized C-Trap Timing of an Orbitrap 240 Mass Spectrometer for High-Throughput Screening and Native MS by IR-MALDESI

Knizner, K. T., Bagley, M. C., Garrard, K. P., Hauschild, J.-P., Pu, F., Elsen, N. L., … Muddiman, D. C. (2022, January 24). Journal of the American Society for Mass Spectrometry, Vol. 2.

By: K. Knizner n, M. Bagley n, K. Garrard n, J. Hauschild*, F. Pu*, N. Elsen*, J. Williams*, D. Muddiman n

author keywords: high-throughput screening; IR-MALDESI; Orbitrap mass spectrometer; C-trap
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
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Sources: Web Of Science, NC State University Libraries
Added: March 7, 2022

2022 article

Phosphorylation-dependent proteome of Marcks in ependyma during aging and behavioral homeostasis in the mouse forebrain

Muthusamy, N., Williams, T. I., Ryan, O. T., Brudvig, J. J., Adler, K. B., Weimer, J. M., … Ghashghaei, H. T. (2022, January 24). GeroScience, Vol. 1, pp. 2077–2094.

author keywords: Aging; Behavior; Clca3; Ependymal cells; Forebrain; Marcks; Mass spectrometry; Neuroinflammation
MeSH headings : Mice; Animals; Phosphorylation; Ependyma / metabolism; Intracellular Signaling Peptides and Proteins / metabolism; Myristoylated Alanine-Rich C Kinase Substrate / metabolism; Proteome / metabolism; Membrane Proteins / metabolism; Protein Kinase C / metabolism; Homeostasis; Prosencephalon / metabolism; Mammals / metabolism
topics (OpenAlex): Neuroscience and Neuropharmacology Research; Neuroinflammation and Neurodegeneration Mechanisms; Alzheimer's disease research and treatments
TL;DR: This study provides first line of evidence for potential mechanisms that may mediate differential Marcks functions in young and old ECs, and their effect on forebrain homeostasis during aging. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 31, 2022

2022 article

Transforming a Mid-infrared Laser Profile from Gaussian to a Top-Hat with a Diffractive Optical Element for Mass Spectrometry Imaging

Bai, H., Manni, J. G., & Muddiman, D. C. (2022, December 21). Journal of the American Society for Mass Spectrometry, Vol. 34, pp. 10–16.

By: H. Bai n, J. Manni & D. Muddiman n

author keywords: diffractive optical element; top-hat; infrared; IR-MALDESI; 3D MSI
MeSH headings : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Electrospray Ionization / methods; Lasers; Light
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Spectroscopy and Laser Applications; Laser-Matter Interactions and Applications
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7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023

2021 article

In situ detection of fatty acid C=C positional isomers by coupling on‐tissue mCPBA epoxidation with infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry

Tu, A., Garrard, K. P., Said, N., & Muddiman, D. C. (2021, May 4). Rapid Communications in Mass Spectrometry, Vol. 7.

By: A. Tu n, K. Garrard n, N. Said* & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: The on-tissue mCPBA epoxidation was implemented into an ambient MS imaging workflow to offer a rapid and simple way for in situ identification and relative quantification of double bond positional isomers without the requirement for instrument modification. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 6, 2021

2021 article

Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Muddiman, D. C. (2021, November 29). Metabolites, Vol. 12.

By: D. Muddiman n

author keywords: metabolites; dicationic reagent; ambient ionization; IR-MALDESI; mass spectrometry imaging
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
Sources: Web Of Science, NC State University Libraries
Added: January 10, 2022

2021 article

Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa

Lin, C.-Y., Sun, Y., Song, J., Chen, H.-C., Shi, R., Yang, C., … Chiang, V. L. (2021, October 6). Frontiers in Plant Science, Vol. 12.

By: C. Lin n, Y. Sun*, J. Song n, H. Chen n, R. Shi n, C. Yang n, J. Liu n, S. Tunlaya-Anukit n ...

Contributors: C. Lin n, H. Chen n, R. Shi n, C. Yang n, J. Liu n, S. Tunlaya-Anukit n, Y. Lin n, R. Sederoff n ...

author keywords: protein interaction; monolignol biosynthesis; wood formation; Populus trichocarpa; BiFC; metabolic flux
topics (OpenAlex): Plant Gene Expression Analysis; Lignin and Wood Chemistry; Biofuel production and bioconversion
TL;DR: It is proposed that Ptr4CL/PtrHCT complexes modulate the metabolic flux of CoA ligation for monolignol biosynthesis during wood formation in P. trichocarpa. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 8, 2021

2021 article

GlycoHunter: An Open-Source Software for the Detection and Relative Quantification of INLIGHT-Labeled N-Linked Glycans

Kalmar, J. G., Garrard, K. P., & Muddiman, D. C. (2021, January 8). Journal of Proteome Research, Vol. 20, pp. 1855–1863.

By: J. Kalmar n, K. Garrard n & D. Muddiman n

author keywords: glycomics; INLIGHT; N-linked glycans; data analysis; open source; software
MeSH headings : Computational Biology; Glycomics; Polysaccharides; Software
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: GlycoHunter, a user-friendly software created in MATLAB that enables researchers to accurately and efficiently process MS1 glycomics data where a NAT and SIL pair is generated for relative quantification, including but not limited to, INLIGHT. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 3, 2021

2021 article

Investigations of β‐carotene radical cation formation in infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI)

Bagley, M. C., & Muddiman, D. C. (2021, May 26). Rapid Communications in Mass Spectrometry.

By: M. Bagley n & D. Muddiman n

MeSH headings : Cations / analysis; Cations / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry; beta Carotene / analysis; beta Carotene / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Antioxidant Activity and Oxidative Stress
TL;DR: There are several potential mechanisms for β-carotene radical cationization prior to IR-MALDESI analysis with multiphoton-ionization, thermal degradation, and/or reaction with oxygen, but changing certain aspects of sample conditions can result in significant mixed-mode ionization with competing protonation. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2021

2021 article

Mass Spectrometry Imaging of N-Linked Glycans in a Formalin-Fixed Paraffin-Embedded Human Prostate by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Pace, C. L., Angel, P. M., Drake, R. R., & Muddiman, D. C. (2021, December 3). Journal of Proteome Research, Vol. 1.

By: C. Pace n, P. Angel*, R. Drake* & D. Muddiman n

author keywords: IR-MALDESI; N-linked glycans; mass spectrometry imaging; prostate cancer
MeSH headings : Formaldehyde / chemistry; Humans; Lasers; Male; Paraffin Embedding; Polysaccharides / chemistry; Prostate / chemistry; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications
TL;DR: This work demonstrates the first steps in N-linked glycan imaging of biological tissues by IR-MALDESI MSI, a hybrid ionization technique commonly used for mass spectrometry imaging (MSI) applications. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: February 7, 2022

2021 article

Michael S. Bereman (1981–2021)

Muddiman, D. C., & MacCoss, M. J. (2021, May 5). Journal of the American Society for Mass Spectrometry, Vol. 5.

By: D. Muddiman n & M. MacCoss*

topics (OpenAlex): Various Chemistry Research Topics; Mass Spectrometry Techniques and Applications; History and advancements in chemistry
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9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021

2021 article

Multimodal Mass Spectrometry Imaging of Rat Brain Using IR-MALDESI and NanoPOTS-LC-MS/MS

Pace, C. L., Simmons, J., Kelly, R. T., & Muddiman, D. C. (2021, December 3). Journal of Proteome Research, Vol. 3.

By: C. Pace n, J. Simmons*, R. Kelly* & D. Muddiman n

author keywords: multimodal mass spectrometry imaging; multiomic analyses; IR-MALDESI; nanoPOTS-LC-MS/MS
MeSH headings : Animals; Brain / diagnostic imaging; Chromatography, Liquid / methods; Proteome; Proteomics; Rats; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications
TL;DR: A multiomic pathway integration showed a strong correlation between the two data sets when comparing average abundances of metabolites and corresponding enzymes in each brain region, and the first steps in the creation of a multimodal MSI technique that combines two highly sensitive and complementary imaging platforms are demonstrated. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022

2021 article

Multiple Infusion Start Time Mass Spectrometry Imaging of Dynamic SIL-Glutathione Biosynthesis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Mellinger, A. L., Garrard, K. P., Khodjaniyazova, S., Rabbani, Z. N., Gamcsik, M. P., & Muddiman, D. C. (2021, November 22). Journal of Proteome Research, Vol. 3.

author keywords: IR-MALDESI; mass spectrometry imaging; stable isotope labeling; glutathione; kinetic metabolism
MeSH headings : Animals; Glutathione; Glycine; Lasers; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Sulfur Compounds in Biology; Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications
TL;DR: A study combining a multiple infusion start time protocol, stable isotope labeling technology, infrared matrix-assisted laser desorption electrospray ionization, and high-resolution accurate mass-mass spectrometry imaging to study spatial changes in glutathione kinetics across in sectioned mouse liver tissues is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022

2021 article

Spatially resolved metabolomic characterization of muscle invasive bladder cancer by mass spectrometry imaging

Tu, A., Said, N., & Muddiman, D. C. (2021, July 21). Metabolomics, Vol. 8.

By: A. Tu n, N. Said* & D. Muddiman n

author keywords: Bladder cancer; IR-MALDESI mass spectrometry imaging; Metabolomics; Lipidomics; Intratumor heterogeneity
MeSH headings : Feasibility Studies; Humans; Metabolomics / methods; Muscle, Smooth / metabolism; Muscle, Smooth / pathology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Urinary Bladder Neoplasms / diagnostic imaging; Urinary Bladder Neoplasms / metabolism; Urinary Bladder Neoplasms / pathology
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Cancer, Lipids, and Metabolism; Mass Spectrometry Techniques and Applications
TL;DR: Great promise is demonstrated of IR-MALDESI MSI to identify molecular changes derived from cancer and unveil tumor heterogeneity, which can potentially promote the discovery of clinically relevant biomarkers and allow for applications in precision medicine. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 2, 2021

2021 article

The development and application of matrix assisted laser desorption electrospray ionization: The teenage years

Bagley, M. C., Garrard, K. P., & Muddiman, D. C. (2021, May 24). Mass Spectrometry Reviews, Vol. 5.

By: M. Bagley n, K. Garrard n & D. Muddiman n

author keywords: ambient ionization; direct analysis; FTMS; IR lasers; MALDESI; mass spectrometry imaging; MSiReader
MeSH headings : Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: This review will discuss the developments of MALDESI since its first laboratory demonstration in 2005 to the most recent advances in 2021. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021

2021 article

Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry

Bai, H., Linder, K. E., & Muddiman, D. C. (2021, January 2). Analytical and Bioanalytical Chemistry.

By: H. Bai n, K. Linder n & D. Muddiman n

author keywords: 3D; Mass spectrometry imaging; IR-MALDESI; Skin; Lipids
MeSH headings : Animals; Imaging, Three-Dimensional / methods; Lipids / analysis; Mice; Mice, Hairless; Skin / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: The use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue is described. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021

2021 article

Understanding the electrospray ionization response factors of per- and poly-fluoroalkyl substances (PFAS)

Enders, J. R., O’Neill, G. M., Whitten, J. L., & Muddiman, D. C. (2021, July 21). Analytical and Bioanalytical Chemistry, Vol. 7.

By: J. Enders n, G. O’Neill n, J. Whitten n & D. Muddiman n

author keywords: Electrospray ionization; PFAS; logP; Surface activity
topics (OpenAlex): Per- and polyfluoroalkyl substances research; Mass Spectrometry Techniques and Applications; Toxic Organic Pollutants Impact
TL;DR: A method for predicting the behavior of individual PFAS compounds, including relative retention order in chromatography, is presented which is simply based on the number of fluorine atoms in the molecule as well as the class of the compound. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 2, 2021

2021 article

Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT™ derivatized N-linked glycans in biological samples

Butler, K. E., Kalmar, J. G., Muddiman, D. C., & Baker, E. S. (2021, August 4). Analytical and Bioanalytical Chemistry, Vol. 8.

By: K. Butler n, J. Kalmar n, D. Muddiman n & E. Baker n

author keywords: Glycomics; Ion mobility spectrometry; INLIGHT (TM); N-Linked glycans; LC-IMS-MS
MeSH headings : Chromatography, Liquid; Glycomics / methods; Glycosylation; Humans; Ion Mobility Spectrometry; Mass Spectrometry / methods; Polysaccharides / analysis
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: The combination of IMS and the natural (NAT) and stable-isotope label (SIL) in the INLIGHT™ strategy provided additional confidence for each glycan identification due to the mobility aligned NAT- and SIL-labeled glycans and further capabilities for isomer examinations. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 16, 2021

2020 article

3D Imaging and metabolomic profiling reveal higher neuroactive kavalactone contents in lateral roots and crown root peels of Piper methysticum (kava)

Jaiswal, Y. S., Yerke, A. M., Bagley, M. C., Ekelöf, M., Weber, D., Haddad, D., … Williams, L. L. (2020, September 1). GigaScience, Vol. 9.

author keywords: kava; kavalactones; metabolomics; 3D imaging; mass spectrometry imaging
MeSH headings : Imaging, Three-Dimensional; Kava; Lactones; Metabolomics; X-Ray Microtomography
topics (OpenAlex): Medicinal Plant Extracts Effects; Alkaloids: synthesis and pharmacology; Phytochemistry and Biological Activities
TL;DR: Detailed metabolomic profile and spatio-temporal characteristics of tissues from the roots and stems are characterized using cross-platform metabolomics and a 3D imaging approach to provide mechanistic insights into the social and clinical practice of the use of only peeled roots. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 16, 2020

2020 journal article

A Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns

Journal of the American Society for Mass Spectrometry, 31(12), 2547–2552.

By: K. Garrard n, M. Ekelöf n, S. Khodjaniyazova n, M. Bagley n & D. Muddiman n

author keywords: Mass Spectrometry Imaging; Arbitrary Region of Interest; MALDESI; Vision-System
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Spectroscopy Techniques in Biomedical and Chemical Research
TL;DR: A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns that reduces contamination from off-sample material and significantly reduces acquisition time. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 19, 2021

2020 article

Analysis of neurotransmitters in rat placenta exposed to flame retardants using IR-MALDESI mass spectrometry imaging

Pace, C. L., Horman, B., Patisaul, H., & Muddiman, D. C. (2020, April 16). Analytical and Bioanalytical Chemistry, Vol. 412, pp. 3745–3752.

By: C. Pace n, B. Horman n, H. Patisaul n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Neurotransmitters; Exposomics
MeSH headings : Animals; Female; Flame Retardants / adverse effects; Infrared Rays; Neurotransmitter Agents / analysis; Optical Imaging / instrumentation; Optical Imaging / methods; Placenta / chemistry; Placenta / diagnostic imaging; Pregnancy; Rats; Rats, Wistar; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Toxic Organic Pollutants Impact; Metabolomics and Mass Spectrometry Studies
TL;DR: Changes in the abundance and spatial distribution of neurotransmitters in rat placenta following chemical exposure could be determined using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 1, 2020

2020 article

Comparative Proteomic Analysis of Wild Type and Mutant Lacking an SCF E3 Ligase F-Box Protein in Magnaporthe oryzae

Kalmar, J. G., Oh, Y., Dean, R. A., & Muddiman, D. C. (2020, July 21). Journal of Proteome Research, Vol. 19, pp. 3761–3768.

By: J. Kalmar n, Y. Oh n, R. Dean n & D. Muddiman n

MeSH headings : Ascomycota; F-Box Proteins / genetics; Fungal Proteins / genetics; Magnaporthe / metabolism; Proteomics; Ubiquitin-Protein Ligases / genetics
topics (OpenAlex): Fungal and yeast genetics research; Plant Pathogens and Fungal Diseases; Ubiquitin and proteasome pathways
TL;DR: A label-free quantitative global proteomics technique was used to probe the role of ubiquitination and phosphorylation in the mechanism of how E3 ligase regulates change in virulence of M. oryzae and greatly assist in the selection of future genes for functional studies and enabling mechanistic insight related to virulence. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 5, 2020

2020 journal article

Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications

Journal of the American Society for Mass Spectrometry, 31(3), 642–650.

By: M. Ekelöf n, J. Dodds n, S. Khodjaniyazova n, K. Garrard n, E. Baker n & D. Muddiman n

author keywords: mass spectrometry imaging; ion mobility spectrometry; IR-MALDESI; high-throughput; isomer resolution
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: To increase a range of detectable features in IR-MALDESI experiments, the home-built ion source was integrated with a commercially available drift tube ion mobility spec-trometer-mass spectrometer (IMS-MS) and analyzed small polar molecules, lipids, carbohydrates as well as intact proteins. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID, Web Of Science
Added: March 23, 2020

2020 article

Determination of Optimal Electrospray Parameters for Lipidomics in Infrared-Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging

Bagley, M. C., Ekelöf, M., & Muddiman, D. C. (2020, January 10). Journal of the American Society for Mass Spectrometry, Vol. 31, pp. 319–325.

By: M. Bagley n, M. Ekelöf n & D. Muddiman n

author keywords: IR-MALDESI; mass spectrometry imaging: electrospray ionization; metabolites; lipids
MeSH headings : Animals; Lipidomics / methods; Lipids / analysis; Liver / chemistry; Liver / diagnostic imaging; Molecular Imaging / methods; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: Using these defined parameters, IR-MALDESI positive polarity lipidomics studies can increase lipid abundances 3-fold, with 15% greater coverage, while an abundance increase of 1.5-fold and 10% more coverage can be achieved relative to commonly used parameters in negative polarity. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 10, 2020

2020 article

Direct Analysis of Native N-Linked Glycans by IR-MALDESI

Pace, C. L., & Muddiman, D. C. (2020, June 30). Journal of the American Society for Mass Spectrometry, Vol. 31, pp. 1759–1762.

By: C. Pace n & D. Muddiman n

author keywords: IR-MALDESI; N-linked glycans; glycomics; direct analysis
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 31, 2020

2020 article

Effects of Prenatal Exposure to a Mixture of Organophosphate Flame Retardants on Placental Gene Expression and Serotonergic Innervation in the Fetal Rat Brain

Rock, K. D., Armour, G. S., Horman, B., Phillips, A., Ruis, M., Stewart, A. K., … Patisaul, H. B. (2020, March 30). Toxicological Sciences, Vol. 176, pp. 203–223.

By: K. Rock n, G. Armour n, B. Horman n, A. Phillips*, M. Ruis*, A. Stewart n, D. Jima n, D. Muddiman n, H. Stapleton*, H. Patisaul n

author keywords: endocrine disruptors; endocrine toxicology; flame retardants; metabolome; neurotoxicity; developmental; neurotoxicology; neurotransmitter; developmental toxicity; prenatal; reproductive and developmental toxicology; developmental/teratology
MeSH headings : Animals; Brain / drug effects; Female; Fetal Development; Fetus; Flame Retardants / toxicity; Gene Expression; Humans; Male; Organophosphates; Organophosphorus Compounds; Placenta / metabolism; Plasticizers; Polybrominated Biphenyls; Pregnancy; Prenatal Exposure Delayed Effects; Rats; Rats, Wistar; Serotonin / metabolism
topics (OpenAlex): Toxic Organic Pollutants Impact; Effects and risks of endocrine disrupting chemicals; Carcinogens and Genotoxicity Assessment
TL;DR: Findings suggest that OPFRs have the potential to impact the 5-HTergic system in the fetal forebrain by disrupting placental Trp metabolism. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 14, 2020

2020 article

Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™

Kalmar, J. G., Butler, K. E., Baker, E. S., & Muddiman, D. C. (2020, August 26). Analytical and Bioanalytical Chemistry.

By: J. Kalmar n, K. Butler n, E. Baker n & D. Muddiman n

author keywords: Glycomics; Orbitrap; Sample preparation; INLIGHT (TM); N-linked glycans; HILIC; PGC; Reversed-phase; Chromatography
MeSH headings : Chromatography, Reverse-Phase / methods; Female; Fetuins / chemistry; Glycomics / methods; Glycosylation; Humans; Male; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / chemistry; Polysaccharides / analysis; Tandem Mass Spectrometry / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Proteoglycans and glycosaminoglycans research
TL;DR: To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated and the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)3, was possible. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2020 article

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging analysis of endogenous metabolites in cherry tomatoes

Bagley, M. C., Pace, C. L., Ekelöf, M., & Muddiman, D. C. (2020, January 1). The Analyst, Vol. 145, pp. 5516–5523.

By: M. Bagley*, C. Pace*, M. Ekelöf* & D. Muddiman n

MeSH headings : Lasers; Lipids; Solanum lycopersicum; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: The spatially resolved metabolic profiling of cherry tomatoes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI), a mass spectrometry imaging technique that operates at ambient conditions and requires no sample derivatization, finds endogenous carotenoid hydrocarbons ionized as radical cations. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 31, 2020

2020 article

Lipidomic profiling of single mammalian cells by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Tu, A., & Muddiman, D. C. (2020, September 29). Analytical and Bioanalytical Chemistry.

By: A. Tu n & D. Muddiman n

author keywords: Single-cell analysis; Lipidome; Mass spectrometry; IR-MALDESI; Orbitrap
MeSH headings : Animals; HeLa Cells; Humans; Limit of Detection; Lipidomics; Rats; Reproducibility of Results; Single-Cell Analysis / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Proteomics Techniques and Applications
TL;DR: A rapid and sensitive method for investigating the lipid profiles of isolated single cells using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry (IR-MALDESI-MS), which provides an important basis for future work on differentiation between normal and diseased cells at various developmental states. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 12, 2020

2020 article

Metabolite Profiling Reveals Predictive Biomarkers and the Absence of β-Methyl Amino-l-alanine in Plasma from Individuals Diagnosed with Amyotrophic Lateral Sclerosis

Bereman, M. S., Kirkwood, K. I., Sabaretnam, T., Furlong, S., Rowe, D. B., Guillemin, G. J., … Muddiman, D. C. (2020, May 18). Journal of Proteome Research, Vol. 19, pp. 3276–3285.

author keywords: amyotrophic lateral sclerosis; biomarkers; BMAA; CZE-MS/MS; metabolites; plasma
MeSH headings : Alanine; Amyotrophic Lateral Sclerosis / diagnosis; Biomarkers; Female; Humans; Male; Mass Spectrometry; Metabolome
topics (OpenAlex): Amyotrophic Lateral Sclerosis Research; Cholinesterase and Neurodegenerative Diseases; Neurological diseases and metabolism
TL;DR: The plasma metabolome of 134 patients diagnosed with sporadic amyotrophic lateral sclerosis and 118 individuals deemed healthy was profiled, and two models using the metabolite abundances were developed for the separation of ALS and healthy samples and the prediction of disease progression based on the ALS functional rating score. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2020 journal article

Methods for Cryosectioning and Mass Spectrometry Imaging of Whole-Body Zebrafish

Journal of the American Society for Mass Spectrometry, 31(4), 768–772.

By: W. Stutts n, M. Knuth n, M. Ekelöf n, D. Mahapatra n, S. Kullman n & D. Muddiman n

author keywords: zebrafish; mass spectrometry imaging; cryosectioning; IR-MALDESI; lipids
MeSH headings : Animals; Cryoultramicrotomy / methods; Lipids / analysis; Male; Molecular Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Zebrafish / metabolism
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Physiological and biochemical adaptations; Ion-surface interactions and analysis
TL;DR: The zebrafish (Danio rerio) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease, and infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebra fish tissue sections. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries, ORCID
Added: February 6, 2021

2019 journal article

Artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua

Molecular Plant, 12(5), 704–714.

By: R. Judd n, M. Bagley n, M. Li n, Y. Zhu n, C. Lei n, S. Yuzuak n, M. Ekelöf n, G. Pu n ...

Contributors: R. Judd n, M. Bagley n, M. Li n, Y. Zhu n, C. Lei n, S. Yuzuak n, M. Ekelöf n, G. Pu n ...

author keywords: Artemisia annua; artemisinin; glandular trichome; non-glandular trichome cell
MeSH headings : Artemisia annua / cytology; Artemisia annua / genetics; Artemisia annua / metabolism; Artemisia annua / physiology; Artemisinins / metabolism; Metabolic Engineering; Mutation; Pollination; Trichomes / metabolism
topics (OpenAlex): Plant biochemistry and biosynthesis; Essential Oils and Antimicrobial Activity; Sesquiterpenes and Asteraceae Studies; Pharmacological Effects of Natural Compounds; Malaria Research and Control
TL;DR: It is reported that non-GT cells of self-pollinated inbred A. annua plants can express the artemisinin biosynthetic pathway and leaves of regenerated gl plantlets biosynthesize artemis inin. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2019 journal article

Development of a relative quantification method for infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry imaging of Arabidopsis seedlings

Rapid Communications in Mass Spectrometry, 34(6).

By: M. Bagley n, A. Stepanova n, M. Ekelöf n, J. Alonso n & D. Muddiman n

MeSH headings : Arabidopsis / chemistry; Arabidopsis / genetics; Arabidopsis Proteins / genetics; Indoleacetic Acids / analysis; Indoles / analysis; Mutation; Oximes / analysis; Oxygenases / genetics; Seedlings / chemistry; Seedlings / genetics; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: IAA doping of agarose was demonstrated as a viable technique for relative quantification of metabolites in live seedlings or tissues with similar biological considerations and was shown to be an appropriate sampling surface for IR-MALDESI mass spectrometry imaging of Arabidopsis seedlings. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: March 10, 2020

2019 article

Heterogeneous antiretroviral drug distribution and HIV/SHIV detection in the gut of three species

Thompson, C. G., Rosen, E. P., Prince, H. M. A., White, N., Sykes, C., Cruz, G., … Kashuba, A. D. M. (2019, July 3). Science Translational Medicine, Vol. 11.

By: C. Thompson*, E. Rosen*, H. Prince*, N. White*, C. Sykes*, G. Cruz*, M. Mathews*, C. Deleage* ...

MeSH headings : ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism; Adolescent; Adult; Aged; Animals; Anti-Retroviral Agents / pharmacology; CD3 Complex / metabolism; Female; Gastrointestinal Tract / drug effects; Gastrointestinal Tract / virology; Gene Expression Regulation, Viral / drug effects; HIV / drug effects; HIV / genetics; Humans; Macaca mulatta; Male; Mice; Middle Aged; RNA, Viral / genetics; RNA, Viral / metabolism; Simian Immunodeficiency Virus / drug effects; Simian Immunodeficiency Virus / genetics; Species Specificity; T-Lymphocytes / drug effects; Young Adult
topics (OpenAlex): HIV Research and Treatment; HIV/AIDS Research and Interventions; HIV/AIDS drug development and treatment
TL;DR: There may be gut regions with little to no exposure to antiretroviral drugs, which may result in low-level HIV replication contributing to HIV persistence. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 5, 2019

2019 article

Internal Energy Deposition in Infrared Matrix-Assisted Laser Desorption Electrospray Ionization With and Without the Use of Ice as a Matrix

Tu, A., & Muddiman, D. C. (2019, September 9). Journal of the American Society for Mass Spectrometry, Vol. 30, pp. 2380–2391.

By: A. Tu n & D. Muddiman n

author keywords: Internal energy deposition; IR-MALDESI; Mass spectrometry imaging; Survival yield method; Thermometer ions; Ambient ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical chemistry methods development
TL;DR: Measurements with holo-myoglobin show that IR-MALDESI-MS retains the noncovalently bound heme-protein complexes under both native-like and denaturing conditions, while complete loss of the heme group occurred in denatured ESI-MS, showing that the softness of IR-MalDESI is equivalent or superior to ESI for biomolecules. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 25, 2019

2019 article

Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

Kalmar, J. G., Oh, Y., Dean, R. A., & Muddiman, D. C. (2019, November 23). Analytical and Bioanalytical Chemistry, Vol. 412, pp. 139–147.

By: J. Kalmar n, Y. Oh n, R. Dean n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Orbitrap; Magnaporthe oryzae; Image recognition; Polarity switching
MeSH headings : Hordeum / metabolism; Hordeum / microbiology; Host-Pathogen Interactions; Infrared Rays; Magnaporthe / metabolism; Magnaporthe / physiology; Metabolomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Fermentation and Sensory Analysis; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: Serotonin, a barley defense metabolite, was a compound identified in both positive and negative modes, and metabolites in the melanin pathway, important for infection development of M. oryzae were identified but were unable to be confirmed with MS/MS due to their low abundances. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: December 9, 2019

2019 article

Label‐Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

Salvato, F., Loziuk, P., Kiyota, E., Daneluzzi, G. S., Araújo, P., Muddiman, D. C., & Mazzafera, P. (2019, June 7). PROTEOMICS.

author keywords: drought; RNA regulation; Saccharum spp; shotgun proteomics; transcription factors
MeSH headings : Droughts; Gene Expression Regulation, Plant; Plant Proteins / metabolism; Proteomics; Saccharum / metabolism
topics (OpenAlex): Sugarcane Cultivation and Processing; Biofuel production and bioconversion; Rice Cultivation and Yield Improvement
TL;DR: The integration of nuclei enrichment sample preparation with the shotgun proteomic approach results in great coverage of the sugarcane stem proteome with 5381 protein groups identified, indicating that drought affects negatively the cell wall metabolism. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

2019 article

Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Khodjaniyazova, S., Hanne, N. J., Cole, J. H., & Muddiman, D. C. (2019, January 1). Analytical Methods, Vol. 11, pp. 5929–5938.

By: S. Khodjaniyazova n, N. Hanne n, J. Cole n & D. Muddiman n

MeSH headings : Animals; Diagnostic Imaging; Lasers; Mice; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Spectroscopy Techniques in Biomedical and Chemical Research; Metabolomics and Mass Spectrometry Studies; Ion-surface interactions and analysis
TL;DR: An effective strategy for direct analysis and two-dimensional matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) of mouse bones that underwent no chemical treatments prior to analysis is reported. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 16, 2019

2019 journal article

NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods

Molecular & Cellular Proteomics, 19(1), 11–30.

By: D. Muddiman & E. Hecht (2 connected NC State authors and many others, 162 authors in total )

author keywords: Glycomics; mass spectrometry; fluorescence; glycosylation; glycoproteins; glycan; glycopeptide; interlaboratory study; NISTmAb; reference antibody
MeSH headings : Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / metabolism; Biological Products; Biopharmaceutics / methods; Glycomics / methods; Glycopeptides / metabolism; Glycosylation; Humans; Laboratories; Polysaccharides / metabolism; Protein Processing, Post-Translational; Proteomics / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Monoclonal and Polyclonal Antibodies Research; Analytical Chemistry and Chromatography
TL;DR: A broad-based interlaboratory study of glycosylation profiles of a reference and modified IgG antibody involving 103 reports from 76 laboratories offers a view of the current state-of-the-art for biologic glyCosylation measurement and suggests a clear need for harmonization of gly cosylation analysis methods. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: May 11, 2020

2019 article

Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance

Borges, K. L. R., Salvato, F., Loziuk, P. L., Muddiman, D. C., & Azevedo, R. A. (2019, July 5). Environmental Science and Pollution Research, Vol. 26, pp. 26039–26051.

author keywords: Root proteome; Cd stress; Antioxidant; Redox; Cell wall; Metabolism
MeSH headings : Cadmium / chemistry; Cadmium / metabolism; Cell Wall / metabolism; Drug Tolerance; Genotype; Solanum lycopersicum / metabolism; Plant Roots / metabolism; Proteome / chemistry; Proteome / metabolism; Proteomics; Stress, Physiological / drug effects
topics (OpenAlex): Plant Micronutrient Interactions and Effects; Aluminum toxicity and tolerance in plants and animals; Plant Stress Responses and Tolerance
TL;DR: Findings could shed some light on the molecular basis underlying the Cd stress response in tomato, providing fundamental insights for the development of Cd-safe cultivars. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2019

2019 article

Systematic evaluation of repeatability of IR-MALDESI-MS and normalization strategies for correcting the analytical variation and improving image quality

Tu, A., & Muddiman, D. C. (2019, June 25). Analytical and Bioanalytical Chemistry, Vol. 411, pp. 5729–5743.

By: A. Tu n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Repeatability; Normalization
MeSH headings : Animals; Chickens; Female; Rats; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: Results demonstrated that the majority of normalization approaches benefit data quality to some extent, and local normalization methods significantly outperform their global counterparts, resulting in a reduction of median %RSD up to 22. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 9, 2019

2019 article

Three-Dimensional Imaging with Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry

Bai, H., Khodjaniyazova, S., Garrard, K. P., & Muddiman, D. C. (2019, December 30). Journal of the American Society for Mass Spectrometry, Vol. 31, pp. 292–297.

By: H. Bai n, S. Khodjaniyazova n, K. Garrard n & D. Muddiman n

author keywords: mass spectrometry imaging; 3D; IR-MALDESI; MUPS; HRAM
MeSH headings : Anti-Ulcer Agents / analysis; Citrates / analysis; Imaging, Three-Dimensional / methods; Omeprazole / analysis; Pharmaceutical Preparations / chemistry; Proton Pump Inhibitors / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Starch / analysis; Tablets, Enteric-Coated / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Ion-surface interactions and analysis
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UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 10, 2020

2018 article

CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Yan, X., Liu, J., Kim, H., Liu, B., Huang, X., Yang, Z., … Chiang, V. L. (2018, October 2). New Phytologist, Vol. 222, pp. 244–260.

By: X. Yan*, J. Liu n, H. Kim*, B. Liu*, X. Huang*, Z. Yang n, Y. Lin*, H. Chen n ...

Contributors: X. Yan*, J. Liu n, H. Kim*, B. Liu*, X. Huang*, Z. Yang n, Y. Lin*, H. Chen n ...

author keywords: co-immunoprecipitation; enzyme activity; monolignol biosynthetic pathway; nuclear magnetic resonance (NMR); Populus trichocarpa; stem-differentiating xylem protein
MeSH headings : Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Down-Regulation / genetics; Gene Expression Regulation, Plant; Lignin / metabolism; Magnetic Resonance Spectroscopy; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Populus / genetics; Populus / metabolism; RNA Interference; Recombinant Proteins / metabolism; Xylem / metabolism
topics (OpenAlex): Plant Gene Expression Analysis; Lignin and Wood Chemistry; Biofuel production and bioconversion
TL;DR: Evidence is provided for the formation of PtrCAD1/PtrCCR2 protein complexes in monolignol biosynthesis in planta, and biomolecular fluorescence complementation and pull-down/co-immunoprecipitation experiments supported a hypothesis of P Tr trichocarpa heterodimer formation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 17, 2018

2018 article

A novel integrated strategy for the detection and quantification of the neurotoxin β-N-methylamino-l-alanine in environmental samples

Beri, J., Kirkwood, K. I., Muddiman, D. C., & Bereman, M. S. (2018, February 17). Analytical and Bioanalytical Chemistry, Vol. 410, pp. 2597–2605.

By: J. Beri n, K. Kirkwood n, D. Muddiman n & M. Bereman n

author keywords: BMAA; CZE; Isomer separation; Quantification; HRAM MS/MS; Seafood
MeSH headings : Amino Acids, Diamino / analysis; Animals; Cyanobacteria Toxins; Environmental Monitoring / methods; Environmental Pollutants / analysis; Fishes / metabolism; Food Analysis / methods; Humans; Limit of Detection; Neurotoxins / analysis; Seafood / analysis; Tandem Mass Spectrometry / methods
topics (OpenAlex): Amino Acid Enzymes and Metabolism; Metabolomics and Mass Spectrometry Studies; Polyamine Metabolism and Applications
TL;DR: Interestingly, BMAA was not identified in the free protein fraction but only detected after protein hydrolysis which suggests that BMAA is tightly bound by and/or incorporated into proteins. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 article

Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging

Ekelöf, M., Manni, J., Nazari, M., Bokhart, M., & Muddiman, D. C. (2018, February 17). Analytical and Bioanalytical Chemistry, Vol. 410, pp. 2395–2402.

By: M. Ekelöf n, J. Manni, M. Nazari n, M. Bokhart n & D. Muddiman n

author keywords: Mass spectrometry imaging; Infrared laser; IR-MALDESI; HRAM mass spectrometry
MeSH headings : Animals; Equipment Design; Infrared Rays; Lasers; Mice; Miniaturization / instrumentation; Optical Imaging / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Whole Body Imaging / instrumentation
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Spectroscopy Techniques in Biomedical and Chemical Research; Metabolomics and Mass Spectrometry Studies
TL;DR: The use of a novel miniature 2.94 μm burst-mode laser in IR-MALDESI allows for rapid and sensitive mass spectrometry imaging of a whole mouse, yielding several hundred confident metabolite identifications. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 article

Discovery and quantification of bioactive peptides in fermented cucumber by direct analysis IR-MALDESI mass spectrometry and LC-QQQ-MS

Fideler, J., Johanningsmeier, S. D., Ekelöf, M., & Muddiman, D. C. (2018, July 31). Food Chemistry, Vol. 271, pp. 715–723.

By: J. Fideler n, S. Johanningsmeier n, M. Ekelöf n & D. Muddiman n

author keywords: Lactic acid fermentation; ACE-inhibitory peptides; Isoleucine-proline-proline; Leucine-proline-proline; Valine-proline-proline; Vegetable composition
MeSH headings : Cucumis sativus / chemistry; Fermentation; Peptides / chemistry; Sodium Chloride; Tandem Mass Spectrometry
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Probiotics and Fermented Foods; Meat and Animal Product Quality
TL;DR: It is demonstrated that lactic acid bacteria fermentation can enhance bioactive peptide content in vegetables by 3-5 fold in fermented cucumbers compared with acidified cucumbers. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 16, 2018

2018 article

Discriminating normal regions within cancerous hen ovarian tissue using multivariate hyperspectral image analysis

Lakeh, M. A., Tu, A., Muddiman, D. C., & Abdollahi, H. (2018, November 23). Rapid Communications in Mass Spectrometry, Vol. 33, pp. 381–391.

By: M. Lakeh*, A. Tu n, D. Muddiman n & H. Abdollahi*

MeSH headings : Animals; Chickens / metabolism; Female; Least-Squares Analysis; Multivariate Analysis; Ovarian Neoplasms / chemistry; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / pathology; Ovarian Neoplasms / veterinary; Ovary / chemistry; Ovary / pathology; Poultry Diseases / diagnosis; Poultry Diseases / pathology; Principal Component Analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Spectroscopy Techniques in Biomedical and Chemical Research
TL;DR: Two unsupervised chemometric methods including PCA and MCR-ALS were applied for resolving and visualizing IR-MALDESI-MS data acquired from hen ovary tissues, improving the interpretation of mass spectrometry imaging results. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 18, 2019

2018 journal article

Evaluation of Digital Image Recognition Methods for Mass Spectrometry Imaging Data Analysis

Journal of The American Society for Mass Spectrometry, 29(12), 2467–2470.

By: M. Ekelöf n, K. Garrard n, R. Judd n, E. Rosen*, D. Xie n, A. Kashuba*, D. Muddiman n

Contributors: M. Ekelöf n, K. Garrard n, R. Judd n, E. Rosen*, D. Xie n, A. Kashuba*, D. Muddiman n

author keywords: Mass spectrometry imaging; Image recognition; SSIM
MeSH headings : Algorithms; Animals; Image Processing, Computer-Assisted / methods; Lymph Nodes / chemistry; Lymph Nodes / metabolism; Macaca mulatta; Maraviroc / pharmacokinetics; Mass Spectrometry / methods; Models, Theoretical; Molecular Imaging / methods; Plant Leaves / chemistry; Tissue Distribution
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Spectroscopy and Chemometric Analyses; Advanced Chemical Sensor Technologies
TL;DR: A method for comprehensive, semi-targeted discovery of molecular distributions of interest from mass spectrometry imaging data, using widely available image similarity scoring algorithms to rank images by spatial correlation is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: December 10, 2018

2018 article

IR-MALDESI mass spectrometry imaging of underivatized neurotransmitters in brain tissue of rats exposed to tetrabromobisphenol A

Bagley, M. C., Ekelöf, M., Rock, K., Patisaul, H., & Muddiman, D. C. (2018, October 13). Analytical and Bioanalytical Chemistry, Vol. 410, pp. 7979–7986.

By: M. Bagley n, M. Ekelöf n, K. Rock n, H. Patisaul n & D. Muddiman n

author keywords: IR-MALDESI; Neurotransmitters; Mass spectrometry imaging; Exposomics; Orbitrap
MeSH headings : Animals; Brain; Female; Infrared Rays; Neurotransmitter Agents / chemistry; Polybrominated Biphenyls / toxicity; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Toxic Organic Pollutants Impact
TL;DR: This study serves as a demonstration of a new application of IR-MALDESI, namely that neurotransmitter distributions can be confidently and rapidly imaged without derivatization, and is used for the analysis of neurotransmitters in the brains of rats exposed to TBBPA in gestation and lactation through their mothers. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018

2018 journal article

Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Nature Communications, 9(1), 1579.

By: J. Wang n, M. Matthews n, C. Williams n, R. Shi n, C. Yang n, S. Tunlaya-Anukit n, H. Chen n, Q. Li* ...

Contributors: J. Wang n, M. Matthews n, C. Williams n, R. Shi n, C. Yang n, S. Tunlaya-Anukit n, H. Chen n, Q. Li* ...

MeSH headings : Gene Expression Regulation, Plant; Lignin / biosynthesis; Lignin / genetics; Plants, Genetically Modified / genetics; Populus / genetics; Populus / metabolism; Transcriptome / genetics; Trees / genetics; Trees / metabolism; Wood / chemistry; Wood / physiology; Xylem / metabolism
topics (OpenAlex): Biofuel production and bioconversion; Plant Gene Expression Analysis; Lignin and Wood Chemistry
TL;DR: A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels and may lead to greater understanding of other pathways for improved growth and adaptation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2018 journal article

Jürgen H. Gross: Mass spectrometry: a textbook, 3rd ed.

Analytical and Bioanalytical Chemistry, 410(8), 2051–2052.

By: D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Isotope Analysis in Ecology
TL;DR: To effectively make use of modern mass spectrometry for chemical and biological systems, one needs to understand the principles of the technique including ionization methods, mass analyzer technology, and ion dissociation as well as a basic understanding of isotopic distributions and measuring mass. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2018 article

Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Nazari, M., Bokhart, M. T., Loziuk, P. L., & Muddiman, D. C. (2018, January 1). The Analyst, Vol. 143, pp. 654–661.

By: M. Nazari n, M. Bokhart n, P. Loziuk n & D. Muddiman n

MeSH headings : Animals; Chickens; Chromatography, Liquid; Female; Glutathione / analysis; Ovarian Neoplasms / diagnostic imaging; Ovary / diagnostic imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: A ∼2-fold increase in the concentration of GSH in cancer tissue compared to the healthy tissue was observed using both techniques, and possible reasons for the difference between absolute concentration values obtained using IR-MALDESI QMSI and LC-MS/MS are discussed. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Characterization of the Spectral Accuracy of an Orbitrap Mass Analyzer Using Isotope Ratio Mass Spectrometry

Analytical Chemistry, 90(3), 1897–1906.

By: S. Khodjaniyazova n, M. Nazari n, K. Garrard n, M. Matos, G. Jackson & D. Muddiman n

MeSH headings : Caffeine / analysis; Carbon Isotopes / analysis; Nitrogen Isotopes / analysis; Oligopeptides / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Sulfur Isotopes / analysis
topics (OpenAlex): Isotope Analysis in Ecology; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: It is demonstrated that at optimum absolute ions abundances the Orbitrap can be used to accurately count carbons, nitrogens, and sulfurs in samples with varying masses and absolute monoisotopic ion abundances required for high SA were empirically determined by using the expected and experimental isotopic distributions to calculate the Pearson chi-square test. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2017 journal article

Climate projections of spatial variations in coastal storm surges along the Gulf of Mexico and U.S. east coast

Journal of Ocean University of China, 16(1), 1–7.

author keywords: storm surge; sea surface winds; climate change; regional ocean
topics (OpenAlex): Tropical and Extratropical Cyclones Research; Oceanographic and Atmospheric Processes; Ocean Waves and Remote Sensing
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UN Sustainable Development Goal Categories
13. Climate Action (Web of Science; OpenAlex)
14. Life Below Water (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2017 journal article

Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae

Proteome Science, 15(1).

By: Y. Oh n, S. Robertson n, J. Parker n, D. Muddiman n & R. Dean n

author keywords: Proteomics; Magnaporthe oryzae; Nitrogen starvation; Melanin biosynthesis; Extracellular protein
topics (OpenAlex): Fungal and yeast genetics research; Fungal Biology and Applications; Mycorrhizal Fungi and Plant Interactions
TL;DR: A functional analysis of differentially expressed proteins revealed that during nitrogen starvation nitrogen catabolite repression, melanin biosynthesis, protein degradation and protein translation pathways underwent extensive alterations, consistent with observations of a link between nitrogen starvation and the development of pathogenicity in M. oryzae. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2017 journal article

Cover Image

Rapid Communications in Mass Spectrometry, 31(22), i-i.

By: M. Nazari, M. Ekelöf, S. Khodjaniyazova, N. Elsen, J. Williams & D. Muddiman*

topics (OpenAlex):
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2017 article

DRILL: An Electrospray Ionization-Mass Spectrometry Interface for Improved Sensitivity via Inertial Droplet Sorting and Electrohydrodynamic Focusing in a Swirling Flow

Kottke, P. A., Lee, J. Y., Jonke, A. P., Seneviratne, C. A., Hecht, E. S., Muddiman, D. C., … Fedorov, A. G. (2017, June 14). Analytical Chemistry, Vol. 89, pp. 8981–8987.

MeSH headings : Angiotensin I / analysis; Angiotensin I / metabolism; Angiotensin II / analysis; Angiotensin II / metabolism; Chromatography, High Pressure Liquid; Humans; Isotope Labeling; Limit of Detection; Peptides / analysis; Peptides / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Electrohydrodynamics and Fluid Dynamics; Laser-induced spectroscopy and plasma
TL;DR: This work experimentally demonstrates sensitivity improvement obtained via vortex-induced inertial sorting of electrosprayed droplets/ions: one possible mode of DRILL operation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Demonstration of hydrazide tagging for O-glycans and a central composite design of experiments optimization using the INLIGHT™ reagent

King, S. R., Hecht, E. S., & Muddiman, D. C. (2017, December 26). Analytical and Bioanalytical Chemistry, Vol. 410, pp. 1409–1415.

By: S. King n, E. Hecht n & D. Muddiman n

author keywords: O-glycosylation; Hydrazide tag; Rapid derivatization; Design of experiments
MeSH headings : Chromatography, Liquid; Glycoproteins / chemistry; Hydrazines / chemistry; Mass Spectrometry; Polysaccharides / chemistry
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Monoclonal and Polyclonal Antibodies Research
TL;DR: This work has taken the INLIGHT™ strategy for N-linked glycan derivatization and adopted it for use with O-linked glycans, demonstrating successful hydrazide tagging of O-glycans for the first time. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI

Analytical and Bioanalytical Chemistry, 410(3), 953–962.

By: M. Nazari n, A. Malico n, M. Ekelöf n, S. Lund n, G. Williams n & D. Muddiman n

author keywords: Terpenes; IR-MALDESI; SESI; Direct analysis; Biological buffers; Q Exactive Plus
MeSH headings : Alkenes / analysis; Buffers; Infrared Rays; Ions / analysis; Monocyclic Sesquiterpenes; Sesquiterpenes / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Terpenes / analysis
topics (OpenAlex): Plant biochemistry and biosynthesis; Metabolomics and Mass Spectrometry Studies; Microbial Metabolic Engineering and Bioproduction
TL;DR: By coupling the two techniques and doping the electrospray solvent with silver ions, olefinic terpenes of different classes and varying degrees of volatility were directly analyzed from a biological buffer system with no sample workup steps. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 article

Direct screening of enzyme activity using infrared matrix‐assisted laser desorption electrospray ionization

Nazari, M., Ekelöf, M., Khodjaniyazova, S., Elsen, N. L., Williams, J. D., & Muddiman, D. C. (2017, August 25). Rapid Communications in Mass Spectrometry, Vol. 31, pp. 1868–1874.

MeSH headings : Drug Discovery / methods; Enzyme Assays / methods; High-Throughput Screening Assays / methods; Isocitrate Dehydrogenase / metabolism; Isocitrates / metabolism; Ketoglutaric Acids / metabolism; Models, Chemical; NADP / metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: The utility of infrared matrix-assisted laser desorption electrospray ionization for monitoring an enzymatic reaction directly from a biological buffer system with no sample cleanup and at high throughput is presented. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution

Journal of The American Society for Mass Spectrometry, 28(10), 2099–2107.

By: M. Bokhart n, J. Manni, K. Garrard n, M. Ekelöf n, M. Nazari n & D. Muddiman n

author keywords: Mass spectrometry imaging; IR-MALDESI; Tissue imaging; Spatial resolution; Laser focusing
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Ion-surface interactions and analysis
TL;DR: This work presents MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) at 50 μm resolution, using an adjustable iris, beam expander, and an aspherical focusing lens to reduce tissue ablation diameters for MSI at high resolution. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2017 article

IR-MALDESI method optimization based on time-resolved measurement of ion yields

Ekelöf, M., & Muddiman, D. C. (2017, August 29). Analytical and Bioanalytical Chemistry, Vol. 410, pp. 963–970.

By: M. Ekelöf n & D. Muddiman n

author keywords: IR-MALDESI; Electrospray post-ionization; Mass spectrometry imaging; Laser ablation; Q Exactive
MeSH headings : Animals; Brain Chemistry; Cholesterol / analysis; Ions / analysis; Laser Therapy / methods; Lipids / analysis; Liver / chemistry; Mice; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Pesticide Residue Analysis and Safety; Analytical Chemistry and Chromatography
TL;DR: In a model imaging experiment using infrared matrix-assisted laser desorption electrospray ionization, fixing the injection time at the minimum duration required to capture all ions generated by the last laser pulse in a sequence is shown to maximize target ion abundances. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Imaging antiretroviral distribution within gastrointestinal tissues across pre-clinical species: implications for hiv eradication

Clinical Pharmacology & Therapeutics, Vol. 101, pp. S17–17.

By: C. Thompson, E. Rosen, M. Mathews, N. White, C. Sykes, Y. Fedoriw, P. Charlins, L. Mulder ...

Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

MSiReader v1.0: Evolving Open-Source Mass Spectrometry Imaging Software for Targeted and Untargeted Analyses

Journal of The American Society for Mass Spectrometry, 29(1), 8–16.

By: M. Bokhart, M. Nazari, K. Garrard n & D. Muddiman*

author keywords: Mass spectrometry imaging; Mass spectrometry software; Data analysis; Image processing; High mass resolving power; Open-source; Orbitrap
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Isotope Analysis in Ecology
TL;DR: A major update to the mass spectrometry imaging (MSI) software MSiReader is presented, offering a multitude of newly added features critical to MSI analyses, enabling the MSI community to evaluate their data in greater depth and in less time. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2017 article

Mass spectrometric detection of chlorophyll a and the tetrapyrrole secondary metabolite tolyporphin A in the filamentous cyanobacterium HT-58-2. Approaches to high-throughput screening of intact cyanobacteria

Zhang, Y., Zhang, R., Nazari, M., Bagley, M. C., Miller, E. S., Williams, P. G., … Lindsey, J. S. (2017, November 1). Journal of Porphyrins and Phthalocyanines, Vol. 21, pp. 759–768.

By: Y. Zhang n, R. Zhang n, M. Nazari n, M. Bagley n, E. Miller n, P. Williams*, D. Muddiman n, J. Lindsey n

author keywords: IR-MALDESI-FTMS; MS/MS; MALDI-TOF-MS; chlorophyll; bacteriochlorin; high-throughput screening
topics (OpenAlex): Porphyrin and Phthalocyanine Chemistry; Marine and coastal ecosystems; Marine Toxins and Detection Methods
TL;DR: Examination by MALDI-TOF-MS of lipophilic crude extracts of small-scale HT-58-2 samples without chromatographic fractionation enabled semi-quantitation of tolyporphin A over a 41-day growth period. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Perfluorinated alcohol induced coacervates as extraction media for proteomic analysis

McCord, J. P., Muddiman, D. C., & Khaledi, M. G. (2017, June 12). Journal of Chromatography A, Vol. 1523, pp. 293–299.

By: J. McCord n, D. Muddiman n & M. Khaledi*

author keywords: Coacervation; Surfactants; Perfluorinated alcohol; Proteomics; Membrane proteins
MeSH headings : Membrane Proteins / isolation & purification; Propanols / chemistry; Proteome / analysis; Proteomics / methods; Saccharomyces cerevisiae / metabolism; Surface-Active Agents / chemistry
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Protein purification and stability
TL;DR: A novel method for using hexafluoroisopropanol induced coacervates with a variety of surfactants to extract proteins from a yeast whole cell lysate and conduct a global proteomic investigation on the extracted proteins is described. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

The PeptideAtlas of the Domestic Laying Hen

McCord, J., Sun, Z., Deutsch, E. W., Moritz, R. L., & Muddiman, D. C. (2017, February 7). Journal of Proteome Research, Vol. 16, pp. 1352–1363.

By: J. McCord n, Z. Sun*, E. Deutsch*, R. Moritz* & D. Muddiman n

author keywords: biomarkers; chicken; ovarian cancer; PeptideAtlas
MeSH headings : Acute-Phase Proteins / analysis; Animals; Biomarkers, Tumor / analysis; Chickens; Data Mining; Female; Proteome / analysis; Proteomics / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Identification and Quantification in Food; Meat and Animal Product Quality
TL;DR: A PeptideAtlas for the domestic chicken is presented, containing an extensive and robust collection of chicken tissue and plasma samples with substantial value for the chicken proteomics community for protein validation and design of downstream targeted proteome quantitation workflows. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

The scope of Analytical and Bioanalytical Chemistry (ABC)

Analytical and Bioanalytical Chemistry, 410(3), 649–650.

By: H. Cui*, P. Garrigues*, G. Gauglitz*, E. Hilder*, G. Hopfgartner*, D. Muddiman*, A. Roda*, A. Sanz-Medel* ...

topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Genetics, Bioinformatics, and Biomedical Research; Various Chemistry Research Topics
TL;DR: Assessing whether an application is within the scope of Analytical and Bioanalytical Chemistry, it is recommended that authors consider whether the major focus of the manuscript is to advance another field and whether the work demonstrates an innovative contribution to the field. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2017 article

Xylose Migration During Tandem Mass Spectrometry of N-Linked Glycans

Hecht, E. S., Loziuk, P. L., & Muddiman, D. C. (2017, January 26). Journal of the American Society for Mass Spectrometry, Vol. 28, pp. 729–732.

By: E. Hecht n, P. Loziuk n & D. Muddiman n

author keywords: Xylose migration; MS/MS; Glycomics; N-linked glycans; Plants
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: This work reports the second instance of saccharide migration ever to occur for N-glycans, the first empirical observation of this rearrangement, and warrants dissemination so it may be searched in de novo sequencing glycan workflows. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Journal of Visualized Experiments, 3(109).

By: E. Hecht n, J. McCord n & D. Muddiman n

author keywords: Chemistry; Issue 109; Glycomics; proteomics; glycosylation-site analysis; deamidation; hydrazide tagging; relative quantitation
MeSH headings : Animals; Chickens; Chromatography, Liquid / methods; Glycomics / methods; Glycopeptides / analysis; Glycopeptides / blood; Glycosylation; Humans; Mass Spectrometry / methods; Peptides / analysis; Peptides / blood; Polysaccharides / analysis; Polysaccharides / blood; Protein Processing, Post-Translational; Proteins / analysis; Proteomics / methods; Solid Phase Extraction / methods; Swine
topics (OpenAlex): Glycosylation and Glycoproteins Research; Peptidase Inhibition and Analysis; Advanced Proteomics Techniques and Applications
TL;DR: A new in-line high-throughput, unbiased, filter based protocol for quantitative glycomics and proteomics analysis and showed that N-glycans purified on filter and derivatized by hydrophobic hydrazide tags were comparable in terms of abundance and class to those by solid phase extraction (SPE); the latter is considered a gold standard in the field. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2016 journal article

A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

Tree Genetics & Genomes, 12(2).

By: C. Lin n, Q. Li*, S. Tunlaya-Anukit*, R. Shi n, Y. Sun*, J. Wang n, J. Liu n, P. Loziuk n ...

Contributors: C. Lin n, Q. Li*, S. Tunlaya-Anukit*, R. Shi n, Y. Sun*, J. Wang n, J. Liu n, P. Loziuk n ...

author keywords: Lignin polymerization; Populus trichocarpa; Lignin peroxidase; LC-MS/MS; Lignin systems biology
topics (OpenAlex): Enzyme-mediated dye degradation; Lignin and Wood Chemistry; Plant Gene Expression Analysis
TL;DR: Differentially expressed gene (DEG) analysis of PtrPO21 downregulated transgenics identified a significant overexpression of PtPrx35, suggesting a compensatory effect within the peroxidase family. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science, ORCID
Added: August 6, 2018

2016 article

Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease

Heaven, M. R., Flint, D., Randall, S. M., Sosunov, A. A., Wilson, L., Barnes, S., … Brenner, M. (2016, May 19). Journal of Proteome Research, Vol. 15, pp. 2265–2282.

By: M. Heaven, D. Flint, S. Randall n, A. Sosunov, L. Wilson, S. Barnes, J. Goldman, D. Muddiman n, M. Brenner

author keywords: Rosenthal fiber; Alexander disease; protein aggregates; neurodegenerative disease; astrocyte; proteomics; mass spectrometry (MS); stress granules
MeSH headings : Alexander Disease; Animals; Astrocytes; Cyclin D2 / analysis; DEAD-box RNA Helicases / analysis; GTP-Binding Proteins / analysis; Humans; Immunohistochemistry; Mice; Neoplasm Proteins / analysis; Neuropeptides / analysis; Protein Aggregates; Protein Aggregation, Pathological; Proteome / analysis; RNA Helicases / analysis; Receptors for Activated C Kinase; Receptors, Cell Surface / analysis
topics (OpenAlex): RNA regulation and disease; RNA and protein synthesis mechanisms; RNA Research and Splicing
TL;DR: No support was obtained for the sequestration hypothesis for AxD, but the association of several of the RF proteins with stress granules suggests a role for stressgranules in the origin of RFs. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Direct Analysis of Triterpenes from High-Salt Fermented Cucumbers Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Journal of The American Society for Mass Spectrometry, 28(2), 370–375.

By: M. Ekelöf n, E. McMurtrie n, M. Nazari n, S. Johanningsmeier n & D. Muddiman n

author keywords: Food preservation; IR-MALDESI; Mass spectrometry imaging; Phytosterols; Direct analysis; HRAM; Fermented vegetable composition; Q Exactive
MeSH headings : Cucumis sativus / chemistry; Fermented Foods / analysis; Food Analysis / methods; Food Storage / methods; Image Processing, Computer-Assisted; Sodium Chloride; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry; Triterpenes / analysis; Triterpenes / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2016 article

Enhanced Lipidome Coverage in Shotgun Analyses by using Gas-Phase Fractionation

Nazari, M., & Muddiman, D. C. (2016, August 25). Journal of the American Society for Mass Spectrometry, Vol. 27, pp. 1735–1744.

By: M. Nazari n & D. Muddiman n

author keywords: Shotgun lipidomics; Gas-phase fractionation; Lipids; Hen model; Lipid metabolism; Polarity switching; Q Exactive Plus
MeSH headings : Animals; Chickens; Female; Glycerophospholipids; Ions; Lipids / chemistry; Tandem Mass Spectrometry
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications
TL;DR: This comprehensive analysis of the lipidome can serve as a species- and tissue-specific database for confident identification of other MS-based datasets, such as mass spectrometry imaging. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

Examining ubiquitinated peptide enrichment efficiency through an epitope labeled protein

Parker, J., Oh, Y., Moazami, Y., Pierce, J. G., Loziuk, P. L., Dean, R. A., & Muddiman, D. C. (2016, August 24). Analytical Biochemistry, Vol. 512, pp. 114–119.

By: J. Parker n, Y. Oh n, Y. Moazami n, J. Pierce n, P. Loziuk n, R. Dean n, D. Muddiman n

author keywords: Ubiquitination; Gly-Gly enrichment; Enrichment efficiency
MeSH headings : Antibodies, Monoclonal, Murine-Derived / chemistry; Epitopes / chemistry; Muramidase / chemistry; Ubiquitinated Proteins / chemistry; Ubiquitination
topics (OpenAlex): Ubiquitin and proteasome pathways; Advanced Proteomics Techniques and Applications; Glycosylation and Glycoproteins Research
TL;DR: It is shown that when conjugated to a lysine containing protein, such as lysozyme, it can be applied as a standard protein to examine ubiquitinated peptide enrichment within a complex background. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

Sarkar, P., Mischler, A., Randall, S. M., Collier, T. S., Dorman, K. F., Boggess, K. A., … Rao, B. M. (2016, July 5). Journal of Proteome Research, Vol. 15, pp. 2433–2444.

By: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n, B. Rao n

Contributors: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n, B. Rao n

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
topics (OpenAlex): Epigenetics and DNA Methylation; Pluripotent Stem Cells Research; Renal and related cancers
TL;DR: The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2016 article

Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

Bokhart, M. T., & Muddiman, D. C. (2016, January 1). The Analyst, Vol. 141, pp. 5236–5245.

By: M. Bokhart n & D. Muddiman n

MeSH headings : Histocytological Preparation Techniques; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical Chemistry and Chromatography
TL;DR: Challenges and limitations of MSI using IR-MALDESI are addressed in the context of the bioimaging field and valuable information is provided for targeted, label-free drug distribution studies and untargeted metabolomic datasets. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

N-linked glycosite profiling and use of Skyline as a platform for characterization and relative quantification of glycans in differentiating xylem of Populus trichocarpa

Loziuk, P. L., Hecht, E. S., & Muddiman, D. C. (2016, August 4). Analytical and Bioanalytical Chemistry, Vol. 409, pp. 487–497.

By: P. Loziuk n, E. Hecht n & D. Muddiman n

author keywords: N-linked glycosite; Skyline; Populus trichocarpa; Monolignol; N-linked glycans
MeSH headings : Computational Biology / standards; Computational Biology / trends; Polysaccharides / analysis; Populus / chemistry; Xylem / chemistry
topics (OpenAlex): Biofuel production and bioconversion; Glycosylation and Glycoproteins Research; Plant Gene Expression Analysis
TL;DR: This study presents a novel workflow for identification of glycans using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy developed in the lab, and implements Skyline for relative glycan quantification for improved accuracy and precision of quantitative measurements over current processing tools. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

Optimizing Mass Spectrometry Analyses: A Tailored Review on the Utility of Design of Experiments

Hecht, E. S., Oberg, A. L., & Muddiman, D. C. (2016, March 7). Journal of the American Society for Mass Spectrometry, Vol. 27, pp. 767–785.

By: E. Hecht n, A. Oberg* & D. Muddiman n

author keywords: Design of experiments; Mass spectrometry; Tutorial; Optimization; Modeling
MeSH headings : Mass Spectrometry; Models, Statistical; Research Design
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Pesticide Residue Analysis and Safety; Analytical Chemistry and Chromatography
TL;DR: This review provides a tutorial-like guide into the selection of DOE for MS experiments, the practices for modeling and optimization of response variables, and the available software tools that support DOE implementation in any laboratory. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

Recent advances in glycomics, glycoproteomics and allied topics

Mechref, Y., & Muddiman, D. C. (2016, December 8). Analytical and Bioanalytical Chemistry, Vol. 409, pp. 355–357.

By: Y. Mechref* & D. Muddiman n

topics (OpenAlex): Glycosylation and Glycoproteins Research
TL;DR: The understanding of the attributes of glycans in biological functions and the development and progression of diseases has been facilitated by mass-spectrometry-based glycomics and glycoproteomics, which are dynamic and are rapidly evolving through innovative technologies and bioinformatics. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

TransOmic analysis of forebrain sections in Sp2 conditional knockout embryonic mice using IR-MALDESI imaging of lipids and LC-MS/MS label-free proteomics

Analytical and Bioanalytical Chemistry, 408(13), 3453–3474.

By: P. Loziuk n, F. Meier n, C. Johnson n, H. Ghashghaei n & D. Muddiman n

author keywords: IR-MALDESI; Proteomics; Lipidomics; Mass spectrometry imaging; Embryonic brain
MeSH headings : Animals; Chromatography, Liquid / methods; Lipids / analysis; Mass Spectrometry / methods; Mice; Mice, Knockout; Prosencephalon / embryology; Prosencephalon / metabolism; Sp2 Transcription Factor / genetics; Spectrophotometry, Infrared / methods; Transcriptome
topics (OpenAlex): Lipid Membrane Structure and Behavior; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: An integrated approach for systematic analysis of the “lipidome” in tissue revealed many specific changes to fundamental cellular processes and biosynthetic pathways in the cerebral cortices of Sp2-cKO embryos. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2016 journal article

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Journal of Visualized Experiments, 3(109).

By: M. Nazari n, M. Bokhart n & D. Muddiman n

author keywords: Bioengineering; Issue 109; Mass spectrometry imaging; IR-MALDESI; Tissue analysis; Whole-body imaging; Metabolites; Lipids
MeSH headings : Histocytological Preparation Techniques / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: A robust IR-MALDESI imaging source has been developed, where a tunable mid-IR laser is synchronized with a computer controlled XY translational stage and a high resolving power mass spectrometer. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2015 article

4-Coumaroyl and Caffeoyl Shikimic Acids Inhibit 4-Coumaric Acid:Coenzyme A Ligases and Modulate Metabolic Flux for 3-Hydroxylation in Monolignol Biosynthesis of Populus trichocarpa

Lin, C.-Y., Wang, J. P., Li, Q., Chen, H.-C., Liu, J., Loziuk, P., … Chiang, V. L. (2015, January 1). Molecular Plant, Vol. 8, pp. 176–187.

By: C. Lin n, J. Wang n, Q. Li*, H. Chen n, J. Liu n, P. Loziuk n, J. Song n, C. Williams n ...

Contributors: C. Lin n, J. Wang n, Q. Li*, H. Chen n, J. Liu n, P. Loziuk n, J. Song n, C. Williams n ...

author keywords: monolignol biosynthesis; Populus trichocarpa; metabolic flux; reaction and inhibition kinetics; LC-MS/MS; 4-coumaroyl and caffeoyl shikimic acids
MeSH headings : Acyl Coenzyme A / metabolism; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Hydroxylation / drug effects; Plant Proteins / metabolism; Populus / drug effects; Populus / enzymology; Populus / metabolism; Propionates; Shikimic Acid / pharmacology
topics (OpenAlex): Biochemical and biochemical processes; Plant Gene Expression Analysis; Lignin and Wood Chemistry
TL;DR: 4-coumaroyl and caffeoyl shikimic acids are inhibitors of Ptr4CL3 and Ptr 4CL5, which can reduce lignin content in a number of plant species and play significant regulatory roles when these inhibitors accumulate. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 article

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

Sarkar, P., Randall, S. M., Collier, T. S., Nero, A., Russell, T. A., Muddiman, D. C., & Rao, B. M. (2015, February 11). Journal of Biological Chemistry, Vol. 290, pp. 8834–8848.

By: P. Sarkar*, S. Randall*, T. Collier*, A. Nero, T. Russell n, D. Muddiman*, B. Rao*

Contributors: P. Sarkar*, S. Randall*, T. Collier*, A. Nero, T. Russell n, D. Muddiman*, B. Rao*

MeSH headings : Activins / metabolism; Base Sequence; Cell Differentiation; Cell Lineage; DNA Methylation; DNA Primers; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Ephrin-B2 / genetics; Humans; Nodal Protein / metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Signal Transduction; Trophoblasts / cytology; Trophoblasts / metabolism
topics (OpenAlex): Pluripotent Stem Cells Research; Renal and related cancers; Mesenchymal stem cell research
TL;DR: It is shown that activin/nodal signaling switches the terminal fate of these hESC-derived trophoblasts, providing a model system that allows for targeted derivation of extravillous trophoblast and syncytiotrophoblasts. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 article

Analysis of Antiretrovirals in Single Hair Strands for Evaluation of Drug Adherence with Infrared-Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging

Rosen, E. P., Thompson, C. G., Bokhart, M. T., Prince, H. M. A., Sykes, C., Muddiman, D. C., & Kashuba, A. D. M. (2015, December 21). Analytical Chemistry, Vol. 88, pp. 1336–1344.

By: E. Rosen*, C. Thompson*, M. Bokhart n, H. Prince*, C. Sykes*, D. Muddiman n, A. Kashuba*

MeSH headings : Anti-Retroviral Agents / analysis; Hair / chemistry; Infrared Rays; Molecular Structure; Optical Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
topics (OpenAlex): HIV/AIDS drug development and treatment; Mass Spectrometry Techniques and Applications; HIV Research and Treatment
TL;DR: Evaluated the detectability of HIV antiretrovirals in hair from a range of drug classes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) with 100 μm resolution and an MSI method to quantify the melanin biomarker pyrrole-2,3,5-tricarboxylic acid (PTCA) was evaluated. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT PlasmaN-Glycans

Hecht, E. S., McCord, J. P., & Muddiman, D. C. (2015, June 18). Analytical Chemistry, Vol. 87, pp. 7305–7312.

By: E. Hecht n, J. McCord n & D. Muddiman n

MeSH headings : Animals; Chickens; Equipment Design; Mass Spectrometry / instrumentation; Polysaccharides / blood; Polysaccharides / isolation & purification; Solid Phase Extraction / instrumentation
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Carbohydrate Chemistry and Synthesis
TL;DR: High-throughput, quantitative processing of N-linked glycans would facilitate large-scale studies correlating the glycome with disease and open the field to basic and applied researchers by coupling filter-aided-N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags (INLIGHT) for analysis of plasma. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Elucidation of Xylem-Specific Transcription Factors and Absolute Quantification of Enzymes Regulating Cellulose Biosynthesis in Populus trichocarpa

Loziuk, P. L., Parker, J., Li, W., Lin, C.-Y., Wang, J. P., Li, Q., … Muddiman, D. C. (2015, August 27). Journal of Proteome Research, Vol. 14, pp. 4158–4168.

By: P. Loziuk n, J. Parker n, W. Li n, C. Lin n, J. Wang n, Q. Li*, R. Sederoff n, V. Chiang n, D. Muddiman n

Contributors: P. Loziuk n, J. Parker n, W. Li n, C. Lin n, J. Wang n, Q. Li*, R. Sederoff n, V. Chiang n, D. Muddiman n

author keywords: shotgun discovery proteomics; absolute quantification; PC-IDMS; cellulose biosynthesis; transcription factor; targeted mass spectrometiry; SRM
MeSH headings : Carbohydrate Metabolism; Cellulose / biosynthesis; Cellulose / genetics; Chromatography, Liquid; Gene Expression Regulation, Plant; Molecular Sequence Annotation; Plant Proteins / genetics; Plant Proteins / isolation & purification; Plant Proteins / metabolism; Populus / genetics; Populus / metabolism; Proteome / genetics; Proteome / isolation & purification; Proteome / metabolism; Proteomics; Tandem Mass Spectrometry; Transcription Factors / genetics; Transcription Factors / isolation & purification; Transcription Factors / metabolism; Wood / chemistry; Wood / metabolism; Xylem / genetics; Xylem / metabolism
topics (OpenAlex): Polysaccharides and Plant Cell Walls; Plant Gene Expression Analysis; Advanced Cellulose Research Studies
TL;DR: The study described herein sought to identify the proteins directly involved in cellulose biosynthesis during wood formation in Populus trichocarpa along with known xylem-specific transcription factors involved in regulating these key proteins. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 article

Global Proteomic Analysis of Functional Compartments in Immature Avian Follicles Using Laser Microdissection Coupled to LC-MS/MS

Nepomuceno, A. I., Muddiman, D. C., & Petitte, J. N. (2015, July 27). Journal of Proteome Research, Vol. 9, pp. 3912–3923.

By: A. Nepomuceno n, D. Muddiman n & J. Petitte n

author keywords: microdissection; proteomics; ovary; follicle; avian; chicken; yolk; steroidogenesis; vitellogenin
MeSH headings : Animals; Avian Proteins / analysis; Avian Proteins / chemistry; Chickens; Chromatography, Liquid; Female; Laser Capture Microdissection / methods; Ovarian Follicle / chemistry; Proteome / analysis; Proteome / chemistry; Proteomics / methods; Tandem Mass Spectrometry
topics (OpenAlex): Meat and Animal Product Quality; Advanced Proteomics Techniques and Applications; Sperm and Testicular Function
TL;DR: A global proteome for SWF, white yolk, and ovarian stroma of the avian ovary is provided that can be used as a baseline for future studies and verifies that the coupling of LMD with proteomic analysis can be use to evaluate proteins from small, physiologically functional compartments of complex tissue. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 article

In-depth LC-MS/MS analysis of the chicken ovarian cancer proteome reveals conserved and novel differentially regulated proteins in humans

Nepomuceno, A. I., Shao, H., Jing, K., Ma, Y., Petitte, J. N., Idowu, M. O., … Hawkridge, A. M. (2015, July 9). Analytical and Bioanalytical Chemistry, Vol. 9, pp. 6851–6863.

author keywords: Mass spectrometry; Proteomics; Ovarian Cancer; Chicken; Ovostatin 2; OVOS2
MeSH headings : Amino Acid Sequence; Animals; Chickens; Chromatography, Liquid / methods; Conserved Sequence; Female; Gene Expression Regulation, Neoplastic; Humans; Mass Spectrometry / methods; Molecular Sequence Data; Neoplasm Proteins / metabolism; Ovarian Neoplasms / metabolism; Proteome / chemistry; Proteome / metabolism; Species Specificity
topics (OpenAlex): S100 Proteins and Annexins; Advanced Proteomics Techniques and Applications; Protease and Inhibitor Mechanisms
TL;DR: Analysis of the ovarian cancer proteome of the chicken provides the first evidence for involvement of OVOS2 in the pathogenesis of both chicken and human ovarian cancer. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 article

Influence of C-Trap Ion Accumulation Time on the Detectability of Analytes in IR-MALDESI MSI

Rosen, E. P., Bokhart, M. T., Nazari, M., & Muddiman, D. C. (2015, September 28). Analytical Chemistry, Vol. 87, pp. 10483–10490.

By: E. Rosen n, M. Bokhart n, M. Nazari n & D. Muddiman n

MeSH headings : Ions / chemistry; Lasers; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Infrared; Time Factors; Xenobiotics / analysis
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: Reducing the C-trap ion accumulation time resulted in increased ion abundance by up to 3 orders of magnitude for analytes ranging from xenobiotics to endogenous lipids, and facilitated the reduction of voxel-to-voxel variability by more than 3-fold. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Influence of Desorption Conditions on Analyte Sensitivity and Internal Energy in Discrete Tissue or Whole Body Imaging by IR-MALDESI

Journal of The American Society for Mass Spectrometry, 26(6), 899–910.

By: E. Rosen n, M. Bokhart n, H. Ghashghaei n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Laser desorption whole body imaging; Lipids; Xenobiotics
MeSH headings : Anesthetics, Local / analysis; Anesthetics, Local / pharmacokinetics; Animals; Cocaine / analysis; Cocaine / pharmacokinetics; Infrared Rays; Lipid Metabolism; Lipids / analysis; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Whole Body Imaging / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: Optimized desorption conditions have been used to conduct an untargeted lipidomic analysis of whole body sagittal sections of neonate mouse, with enhanced lipid coverage received by varying the laser wavelength used for mass spectrometry imaging (MSI). (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 chapter

MALDESI: Fundamentals, Direct Analysis, and MS Imaging

In Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry (pp. 169–182).

By: M. Nazari n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Ion-surface interactions and analysis
TL;DR: The vast experimental space governing the MAL Desorption electrospray ionization process has been investigated thoroughly, while its applications in direct analysis of biomolecules were explored. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2015 journal article

Machine learning reveals sex-specific 17β-estradiol-responsive expression patterns in white perch (Morone americana) plasma proteins

PROTEOMICS, 15(15), 2678–2690.

By: J. Schilling n, A. Nepomuceno n, A. Planchart n, J. Yoder n, R. Kelly n, D. Muddiman n, H. Daniels n, N. Hiramatsu*, B. Reading n

Contributors: J. Schilling n, A. Nepomuceno n, A. Planchart n, J. Yoder n, R. Kelly n, D. Muddiman n, H. Daniels n, N. Hiramatsu*, B. Reading n

author keywords: Animal proteomics; Endocrine disrupting compounds; Machine learning; Plasma; Semiquantitative proteomics; Support vector machines
MeSH headings : Animals; Bass / metabolism; Blood Proteins / metabolism; Blotting, Western; Chromatography, Liquid; Estradiol / pharmacology; Estrogens / pharmacology; Female; Fish Proteins / metabolism; Kininogens / metabolism; Male; Nanotechnology / methods; Proteome / drug effects; Proteome / metabolism; Proteomics / methods; Sex Factors; Support Vector Machine; Tandem Mass Spectrometry; Vitellogenins / metabolism
topics (OpenAlex): Reproductive biology and impacts on aquatic species; Hormonal and reproductive studies; Aquaculture Nutrition and Growth
TL;DR: This is the first report that Eltd1 and Kng1 may be E2‐responsive proteins in fishes and therefore may be useful indicators of estrogen induction in fishes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2015 article

Mass Spectrometry Imaging Reveals Heterogeneous Efavirenz Distribution within Putative HIV Reservoirs

Thompson, C. G., Bokhart, M. T., Sykes, C., Adamson, L., Fedoriw, Y., Luciw, P. A., … Rosen, E. P. (2015, March 3). Antimicrobial Agents and Chemotherapy, Vol. 59, pp. 2944–2948.

By: C. Thompson*, M. Bokhart n, C. Sykes*, L. Adamson*, Y. Fedoriw*, P. Luciw*, D. Muddiman n, A. Kashuba*, E. Rosen n

MeSH headings : Alkynes; Animals; Anti-Retroviral Agents / pharmacokinetics; Benzoxazines / pharmacokinetics; Cyclopropanes; Gray Matter / metabolism; Lymph Nodes / metabolism; Macaca; Mass Spectrometry / methods
topics (OpenAlex): HIV Research and Treatment; HIV/AIDS drug development and treatment; HIV/AIDS Research and Interventions
TL;DR: Intratissue efavirenz distribution was heterogeneous, with the drug concentrating in the lamina propria of the colon, the primary follicles of lymph nodes, and the brain gray matter, the first imaging data of an antiretroviral drug in active viral reservoirs. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Mechanisms of Egg Yolk Formation and Implications on Early Life History of White Perch (Morone americana)

Schilling, J., Loziuk, P. L., Muddiman, D. C., Daniels, H. V., & Reading, B. J. (2015, November 18). PLoS ONE, Vol. 10.

By: J. Schilling n, P. Loziuk n, D. Muddiman n, H. Daniels n & B. Reading n

MeSH headings : Animals; Bass / metabolism; Blotting, Western; Chromatography, Liquid; Egg Proteins / metabolism; Egg Yolk / metabolism; Female; Immunohistochemistry; Oocytes / cytology; Oocytes / metabolism; Ovary / cytology; Receptors, Cell Surface / metabolism; Sexual Maturation; Staining and Labeling; Tandem Mass Spectrometry; Vitellogenins / metabolism
topics (OpenAlex): Reproductive biology and impacts on aquatic species; Aquaculture Nutrition and Growth; Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
TL;DR: The three white perch vitellogenins were quantified accurately and precisely in the liver, plasma, and ovary during pre-vitellogenic oocyte growth using protein cleavage-isotope dilution mass spectrometry (PC-IDMS). (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Phosphoproteome Analysis Links Protein Phosphorylation to Cellular Remodeling and Metabolic Adaptation during Magnaporthe oryzae Appressorium Development

Journal of Proteome Research, 14(6), 2408–2424.

By: W. Franck n, E. Gokce n, S. Randall n, Y. Oh n, A. Eyre n, D. Muddiman n, R. Dean n

author keywords: Magnaporthe oryzae; quantitative phosphoproteomics; appressorium formation; network analysis; transcription factors
MeSH headings : Adaptation, Physiological; Basic Helix-Loop-Helix Transcription Factors / metabolism; Chromatography, Liquid; Fungal Proteins / metabolism; Magnaporthe / metabolism; Magnaporthe / physiology; Oryza / microbiology; Phosphoproteins / metabolism; Phosphorylation; Proteomics; Signal Transduction; Tandem Mass Spectrometry
topics (OpenAlex): Fungal and yeast genetics research; Fungal Biology and Applications; Plant-Microbe Interactions and Immunity
TL;DR: Network analysis incorporating regulation from transcriptomic, proteomic, and phosphoproteomic data revealed new insights into the regulation of the metabolism of conidial storage reserves and phospholipids, autophagy, actin dynamics, and cell wall metabolism during appressorium formation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2015 journal article

Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis

Proceedings of the National Academy of Sciences, 112(27), 8481–8486.

By: J. Wang n, L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, R. Shi n, G. Qu*, D. Muddiman n, R. Sederoff n, V. Chiang n

Contributors: J. Wang n, L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, R. Shi n, G. Qu*, D. Muddiman n, R. Sederoff n, V. Chiang n

author keywords: AldOMT; COMT; lignin; phosphoproteomics; phosphoregulation
MeSH headings : Acrolein / analogs & derivatives; Acrolein / metabolism; Amino Acid Sequence; Binding Sites / genetics; Biocatalysis; Catechols / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Isoenzymes / genetics; Isoenzymes / metabolism; Lignin / biosynthesis; Methyltransferases / genetics; Methyltransferases / metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutation; Phosphoproteins / metabolism; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Populus / enzymology; Populus / genetics; Populus / metabolism; Proteomics / methods; Protoplasts / enzymology; Protoplasts / metabolism; Sequence Homology, Amino Acid; Tandem Mass Spectrometry
topics (OpenAlex): Plant Gene Expression Analysis; Biofuel production and bioconversion; Horticultural and Viticultural Research
TL;DR: It is shown that reversible monophosphorylation at Ser123 or Ser125 acts as an on/off switch for the activity of 5-hydroxyconiferaldehyde O-methyltransferase 2 (PtrAldOMT2), an enzyme central to monolignol biosynthesis for lignification in stem-differentiating xylem of Populus trichocarpa. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science, ORCID
Added: August 6, 2018

2015 article

Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Nazari, M., & Muddiman, D. C. (2015, September 21). The Analyst, Vol. 141, pp. 595–605.

By: M. Nazari n & D. Muddiman n

MeSH headings : Animals; Chickens; Female; Infrared Rays; Molecular Imaging / methods; Ovarian Neoplasms / diagnostic imaging; Ovarian Neoplasms / pathology; Ovary / cytology; Ovary / diagnostic imaging; Ovary / pathology; Solvents / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: In order to obtain a comprehensive metabolite and lipid data, a polarity switching MSI method using infrared matrix assisted laser desorption electrospray ionization (IR-MALDESI) was developed and optimized where the electrosPRay polarity was alternated from one voxel to the next. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples

Hecht, E. S., Scholl, E. H., Walker, S. H., Taylor, A. D., Cliby, W. A., Motsinger-Reif, A. A., & Muddiman, D. C. (2015, September 8). Journal of Proteome Research.

By: E. Hecht*, E. Scholl*, S. Walker*, A. Taylor, W. Cliby*, A. Motsinger-Reif*, D. Muddiman*

author keywords: N-linked glycosylation; INLIGHT; ovarian cancer; relative quantification; cancer biomarker; human plasma
MeSH headings : Biomarkers, Tumor / blood; Carbohydrate Sequence; Case-Control Studies; Chromatography, Liquid / methods; Female; Fucose / blood; Glycosylation; Humans; Hydrazines / chemistry; Molecular Sequence Data; Neoplasm Staging; Ovarian Neoplasms / blood; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / pathology; Polysaccharides / blood; Sialic Acids / blood; Staining and Labeling / methods; Tandem Mass Spectrometry / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Monoclonal and Polyclonal Antibodies Research; Lung Cancer Research Studies
TL;DR: For all N-glycans, the vectors of the effects from stages II-IV were sequentially reversed, suggesting potential biological changes in OVC morphology or in host response. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Wall modified photonic crystal fibre capillaries as porous layer open tubular columns for in-capillary micro-extraction and capillary chromatography

Kazarian, A. A., Rodriguez, E. S., Deverell, J. A., McCord, J., Muddiman, D. C., & Paull, B. (2015, October 24). Analytica Chimica Acta, Vol. 905, pp. 1–7.

By: A. Kazarian n, E. Rodriguez*, J. Deverell*, J. McCord n, D. Muddiman n & B. Paull*

author keywords: Polymer monolith; Porous layer open tubular column; Fused silica photonic crystal fibres; In-capillary extraction; Bioseparations
MeSH headings : Chromatography, Liquid / instrumentation; Chromatography, Liquid / methods; Equipment Design; Photons; Porosity; Reproducibility of Results; Spectrometry, Fluorescence
topics (OpenAlex): Microfluidic and Capillary Electrophoresis Applications; Mass Spectrometry Techniques and Applications; Innovative Microfluidic and Catalytic Techniques Innovation
TL;DR: The modified multi-channel capillaries were applied to the in-capillary micro-extraction of water samples, proving chromatographic potential of the new column format, with run-to-run retention time reproducibility of below 1%. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

What if you could only publish 50 papers your entire career?

Muddiman, D. C. (2015, December 16). Analytical and Bioanalytical Chemistry, Vol. 408, pp. 663–664.

By: D. Muddiman n

topics (OpenAlex): Scientific Computing and Data Management; scientometrics and bibliometrics research
TL;DR: Though this shift to the highest publishable unit (HPU) will not mean every paper is a blockbuster, it would guarantee that each manuscript fulfills one or more of three goals: Regardless of journal type or impact factor, each manuscript should detail a complete and significant study. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Analysis of trace fibers by IR-MALDESI imaging coupled with high resolving power MS

Cochran, K. H., Barry, J. A., Robichaud, G., & Muddiman, D. C. (2014, July 31). Analytical and Bioanalytical Chemistry, Vol. 407, pp. 813–820.

By: K. Cochran n, J. Barry n, G. Robichaud n & D. Muddiman n

author keywords: Mass spectrometry imaging; Forensic science; Trace evidence; Polarity switching imaging; MALDESI
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Forensic Fingerprint Detection Methods
TL;DR: IR-MALDESI was used as a source for mass spectrometry imaging (MSI) of a dyed nylon fiber cluster and single fiber to yield more quantitative structural information about the dye and polymer that can be used for more conclusive comparisons. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Are Presentations at ASMS Conferences Publications?

Muddiman, D. C., & Weintraub, S. T. (2014, January 2). Journal of the American Society for Mass Spectrometry, Vol. 25, pp. 301–302.

By: D. Muddiman n & S. Weintraub*

MeSH headings : Congresses as Topic; Humans; Mass Spectrometry; Publications; Societies, Scientific / organization & administration
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Isotope Analysis in Ecology
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Assessing drug and metabolite detection in liver tissue by UV-MALDI and IR-MALDESI mass spectrometry imaging coupled to FT-ICR MS

Barry, J. A., Groseclose, M. R., Robichaud, G., Castellino, S., & Muddiman, D. C. (2014, May 24). International Journal of Mass Spectrometry, Vol. 377, pp. 448–455.

author keywords: MALDI; IR-MALDESI; Mass spectrometry imaging; Drug distribution
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: The detection of lapatinib as well as several of its metabolites in liver tissue was determined by MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) coupled to high resolving power Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 chapter

Atmospheric Pressure Mass Spectrometry Imaging

In R. A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation (pp. 1–44).

By: J. Barry n, D. Muddiman n & G. Robichaud n

Ed(s): R. Meyers

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Spectroscopy and Laser Applications; Advanced Chemical Physics Studies
Sources: NC State University Libraries, NC State University Libraries
Added: March 8, 2021

2014 journal article

C/EBPα regulates CRL4Cdt2-mediated degradation of p21 in response to UVB-induced DNA damage to control the G1/S checkpoint

Cell Cycle, 13(22), 3602–3610.

By: J. Hall n, M. Bereman n, A. Nepomuceno n, E. Thompson n, D. Muddiman n & R. Smart n

author keywords: C/EBP alpha; CRL4(Cdt2); G(1)/S DNA damage checkpoint; keratinocytes; p21
MeSH headings : Animals; CCAAT-Enhancer-Binding Protein-alpha / genetics; CCAAT-Enhancer-Binding Protein-alpha / metabolism; Cell Cycle Checkpoints / genetics; Cell Cycle Checkpoints / radiation effects; Cyclin-Dependent Kinase Inhibitor p21 / metabolism; DNA Damage / genetics; DNA Damage / radiation effects; G1 Phase Cell Cycle Checkpoints / genetics; Keratinocytes / metabolism; Keratinocytes / radiation effects; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Proteolysis; Ubiquitin-Protein Ligases / genetics; Ubiquitin-Protein Ligases / metabolism; Ultraviolet Rays; p21-Activated Kinases / genetics; p21-Activated Kinases / metabolism
topics (OpenAlex): Cancer-related Molecular Pathways; Ubiquitin and proteasome pathways; DNA Repair Mechanisms
TL;DR: Findings reveal C/EBPα regulates G1/S cell cycle arrest in response to DNA damage via the control of CRL4Cdt2 mediated degradation of p21. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2014 article

Cellular-level mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) by oversampling

Nazari, M., & Muddiman, D. C. (2014, December 8). Analytical and Bioanalytical Chemistry, Vol. 407, pp. 2265–2271.

By: M. Nazari n & D. Muddiman n

author keywords: Mass spectrometry imaging; Infrared; MALDESI; Cellular analysis; Oversampling; Tissue analysis; Lipids
MeSH headings : Cells / chemistry; Cells / metabolism; Cervix Uteri / chemistry; Cervix Uteri / metabolism; Cholesterol / metabolism; Female; Humans; Microtomy; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) imaging of cervical tissue sections was performed using a spot-to-spot distance of 10 μm by utilizing the method of oversampling, where the target plate is moved by a distance that is less than the desOrption radius of the laser. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Compartment Proteomics Analysis of White Perch (Morone americana) Ovary Using Support Vector Machines

Schilling, J., Nepomuceno, A., Schaff, J. E., Muddiman, D. C., Daniels, H. V., & Reading, B. J. (2014, February 4). Journal of Proteome Research, Vol. 13, pp. 1515–1526.

By: J. Schilling n, A. Nepomuceno n, J. Schaff n, D. Muddiman n, H. Daniels n & B. Reading n

author keywords: compartment proteomics; support vector machines; modulated modularity clustering; ovary; oocytes; vitellogenin; transcriptome; mitochondria; alternatively spliced variants
MeSH headings : Animals; Bass / metabolism; Chemical Fractionation; Female; Fish Proteins / analysis; Membrane Proteins / analysis; Mitochondrial Proteins / analysis; Molecular Sequence Annotation; Ovary / chemistry; Ovary / metabolism; Proteomics / methods; Support Vector Machine; Tandem Mass Spectrometry
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Identification and Quantification in Food; Mass Spectrometry Techniques and Applications
TL;DR: The white perch ovary cytosolic and membrane proteomes and transcriptome presented in this study can support future investigations into oogenesis and early embryogenesis ofwhite perch and other members of the genus Morone. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
14. Life Below Water (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa

The Plant Cell, 26(3), 894–914.

By: J. Wang n, P. Naik n, H. Chen n, R. Shi n, C. Lin n, J. Liu n, C. Shuford n, Q. Li n ...

Contributors: J. Wang n, P. Naik n, H. Chen n, R. Shi n, C. Lin n, J. Liu n, C. Shuford n, Q. Li n ...

MeSH headings : Kinetics; Lignin / metabolism; Mass Spectrometry; Plant Proteins / metabolism; Polymorphism, Single Nucleotide; Populus / metabolism; Proteome
topics (OpenAlex): Plant Gene Expression Analysis; Lignin and Wood Chemistry; Biofuel production and bioconversion
TL;DR: A proteomic-based predictive kinetic metabolic-flux model was developed for monolignol biosynthesis in Populus trichocarpa and predicts how pathway enzymes affect lignin content and composition, explains a long-standing paradox regarding the regulation of monolIGNol subunit ratios in lignIn, and reveals novel mechanisms involved in theregulation of lign in biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 article

Establishing ion ratio thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry

Loziuk, P. L., Sederoff, R. R., Chiang, V. L., & Muddiman, D. C. (2014, January 1). The Analyst, Vol. 139, pp. 5439–5450.

By: P. Loziuk n, R. Sederoff n, V. Chiang n & D. Muddiman n

MeSH headings : Chromatography, Liquid; Isotopes / chemistry; Proteins / analysis; Tandem Mass Spectrometry / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: It is suggested that evaluation of the absolute ion abundance for any given transition is necessary in order to effectively implement RA thresholds, and these RA values should be assessed as close as possible to the time at which data is collected for quantification. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis

Rapid Communications in Mass Spectrometry, 28(23), 2511–2522.

By: S. Randall n, I. Koryakina n, G. Williams n & D. Muddiman n

MeSH headings : Acyltransferases / metabolism; Chromatography, Liquid / methods; Filtration; Hydrophobic and Hydrophilic Interactions; Mass Spectrometry / methods; Oligopeptides / chemistry; Oligopeptides / metabolism; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Polyketides / chemistry; Polyketides / metabolism
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Enzyme Structure and Function
TL;DR: The MS signal as a function of nonpolar surface area (NPSA) is explored in order to better understand this bias in electrospray response and to determine relative LC/MS response for peptides. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2014 article

Focus on Advancing High Performance Mass Spectrometry, Honoring Dr. Richard D. Smith, Recipient of the 2013 Award for a Distinguished Contribution in Mass Spectrometry

Baker, E. S., Muddiman, D. C., & Loo, J. A. (2014, October 17). Journal of the American Society for Mass Spectrometry, Vol. 25, pp. 1997–1999.

By: E. Baker*, D. Muddiman n & J. Loo*

Contributors: E. Baker*, D. Muddiman n & J. Loo*

topics (OpenAlex): Mass Spectrometry Techniques and Applications
TL;DR: This special focus issue of the Journal of the American Society for Mass spectrometry celebrates the accomplishments of Dr. Richard D. Smith, the recipient of the 2013ASMS Award for a Distinguished Contribution in Mass Spectrometry. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2014 article

Hierarchical Self‐Assembly of Supramolecular Hydrophobic Metallacycles into Ordered Nanostructures

Zhang, J., Marega, R., Chen, L. J., Wu, N. W., Xu, X. D., Muddiman, D. C., … Yang, H. B. (2014, August 19). Chemistry - An Asian Journal, Vol. 9, pp. 2928–2936.

author keywords: metallacycles; noncovalent interactions; nanostructures; self-assembly; supramolecular chemistry
topics (OpenAlex): Supramolecular Self-Assembly in Materials; Supramolecular Chemistry and Complexes; Metal-Organic Frameworks: Synthesis and Applications
TL;DR: A new series of well-defined metallacycles decorated with long alkyl chains were obtained through metal-ligand interactions, which were capable of aggregating into ordered fibroid or spherical nanostructures on the surface, mostly driven by hydrophobic interactions. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

IR-MALDESI Mass Spectrometry Imaging of Biological Tissue Sections Using Ice as a Matrix

Robichaud, G., Barry, J. A., & Muddiman, D. C. (2014, January 2). Journal of the American Society for Mass Spectrometry, Vol. 25, pp. 319–328.

By: G. Robichaud n, J. Barry n & D. Muddiman n

author keywords: Mass spectrometry imaging; IR-MALDESI; Tissue ablation; Shadowgraphy
MeSH headings : Animals; Brain / cytology; Brain Chemistry; Histocytological Preparation Techniques / methods; Ice; Mice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical Chemistry and Chromatography
TL;DR: With a better understanding of the fundamentals of ablation dynamics and a systematic approach to explore the experimental space, it was possible to improve ion abundance by nearly one order of magnitude. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Mapping Antiretroviral Drugs in Tissue by IR-MALDESI MSI Coupled to the Q Exactive and Comparison with LC-MS/MS SRM Assay

Barry, J. A., Robichaud, G., Bokhart, M. T., Thompson, C., Sykes, C., Kashuba, A. D. M., & Muddiman, D. C. (2014, April 17). Journal of the American Society for Mass Spectrometry, Vol. 25, pp. 2038–2047.

By: J. Barry n, G. Robichaud n, M. Bokhart n, C. Thompson*, C. Sykes*, A. Kashuba*, D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; LC-MS/MS; Drug distribution
MeSH headings : Adenine / analogs & derivatives; Adenine / analysis; Adenine / chemistry; Adenine / pharmacokinetics; Anti-Retroviral Agents / analysis; Anti-Retroviral Agents / chemistry; Anti-Retroviral Agents / pharmacokinetics; Cervix Uteri / chemistry; Cervix Uteri / metabolism; Chromatography, Liquid / methods; Deoxycytidine / analogs & derivatives; Deoxycytidine / analysis; Deoxycytidine / chemistry; Deoxycytidine / pharmacokinetics; Emtricitabine; Female; Histocytochemistry; Humans; Molecular Imaging / methods; Organophosphonates / analysis; Organophosphonates / chemistry; Organophosphonates / pharmacokinetics; Pyrrolidinones / analysis; Pyrrolidinones / chemistry; Pyrrolidinones / pharmacokinetics; Raltegravir Potassium; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry / methods; Tenofovir; Tissue Distribution
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Spectroscopy Techniques in Biomedical and Chemical Research; Metabolomics and Mass Spectrometry Studies
TL;DR: An agreement between the average intensities from the imaging experiment and the absolute quantities for each drug was observed and this correlation between these two techniques serves as a prerequisite to quantitative IR-MALDESI MSI. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Michael Gross: 25 Years of Dedication and Leadership of JASMS (1990–2015)

Muddiman, D. C. (2014, December 23). Journal of the American Society for Mass Spectrometry, Vol. 26, pp. 1–4.

By: D. Muddiman n

topics (OpenAlex): Health and Medical Research Impacts
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Quantitative mass spectrometry imaging of emtricitabine in cervical tissue model using infrared matrix-assisted laser desorption electrospray ionization

Bokhart, M. T., Rosen, E., Thompson, C., Sykes, C., Kashuba, A. D. M., & Muddiman, D. C. (2014, October 15). Analytical and Bioanalytical Chemistry, Vol. 407, pp. 2073–2084.

By: M. Bokhart n, E. Rosen n, C. Thompson*, C. Sykes*, A. Kashuba* & D. Muddiman n

author keywords: Mass spectrometry imaging; IR-MALDESI; Absolute quantification; Drug distribution; HIV; Selected reaction monitoring
MeSH headings : Diagnostic Imaging / instrumentation; Diagnostic Imaging / methods; Female; Humans; Papillomavirus Vaccines / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Uterine Cervical Neoplasms / chemistry; Uterine Cervical Neoplasms / diagnosis
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: A quantitative mass spectrometry imaging technique using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is demonstrated for the antiretroviral (ARV) drug emtricitabine in incubated human cervical tissue, leading to a gain in sensitivity and removal of interferences for several ARV drugs. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 article

Silver dopants for targeted and untargeted direct analysis of unsaturated lipids via infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI)

Meier, F., Garrard, K. P., & Muddiman, D. C. (2014, October 9). Rapid Communications in Mass Spectrometry, Vol. 28, pp. 2461–2470.

By: F. Meier n, K. Garrard n & D. Muddiman n

MeSH headings : Alkenes / chemistry; Dried Blood Spot Testing; Fats, Unsaturated / analysis; Fats, Unsaturated / chemistry; Humans; Male; Silver / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: Silver cationization has been shown to enhance IR-MALDESI sensitivity and selectivity for unsaturated lipids, even when applied to complex samples, and developed tools for peak assignment and mapping of isotopic patterns will clearly benefit future mass spectrometry imaging studies. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling

The Plant Cell, 26(3), 876–893.

By: H. Chen n, J. Song n, J. Wang n, Y. Lin n, J. Ducoste n, C. Shuford n, J. Liu n, Q. Li n ...

Contributors: H. Chen n, J. Song n, J. Wang n, Y. Lin n, J. Ducoste n, C. Shuford n, J. Liu n, Q. Li n ...

MeSH headings : Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Immunoprecipitation; Lignin / biosynthesis; Mass Spectrometry; Models, Biological; Populus / metabolism; Propionates; RNA, Messenger / genetics; Substrate Specificity; Systems Biology
topics (OpenAlex): Biofuel production and bioconversion; Lignin and Wood Chemistry; Plant Gene Expression Analysis; Biochemical and biochemical processes
TL;DR: This work shows that 4CL, an enzyme in monolignol biosynthesis, is found as a heterotetrameric complex of two isoforms in Populus trichocarpa, where the two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact in vivo and in vitro to form a heterosymmetric protein complex. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science, ORCID
Added: August 6, 2018

2013 article

Reply to the Comment on: “Utilizing Artificial Neural Networks in MATLAB to Achieve Parts-Per-Billion Mass Measurement Accuracy with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer” by D. Keith Williams Jr., Alexander L. Kovach, David C. Muddiman, and Kenneth W. Hanck. J. Am. Soc. Mass Spectrom.20, 1303–1310 (2009)

Muddiman, D. C. (2013, December 20). Journal of the American Society for Mass Spectrometry, Vol. 25, pp. 697–697.

By: D. Muddiman n

MeSH headings : Fourier Analysis; Mass Spectrometry / methods; Neural Networks, Computer
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Accurate Identification of Deamidated Peptides in Global Proteomics Using a Quadrupole Orbitrap Mass Spectrometer

Nepomuceno, A. I., Gibson, R. J., Randall, S. M., & Muddiman, D. C. (2013, November 29). Journal of Proteome Research, Vol. 13, pp. 777–785.

By: A. Nepomuceno n, R. Gibson n, S. Randall n & D. Muddiman n

author keywords: deamidation; post-translational modification; quadrupole orbitrap mass spectrometry
MeSH headings : Amides / chemistry; Chromatography, Liquid; Peptides / chemistry; Proteomics; Tandem Mass Spectrometry / instrumentation
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Glycosylation and Glycoproteins Research
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Comparative Proteomic Analysis and IgE Binding Properties of Peanut Seed and Testa (Skin)

White, B. L., Gökce, E., Nepomuceno, A. I., Muddiman, D. C., Sanders, T. H., & Davis, J. P. (2013, March 27). Journal of Agricultural and Food Chemistry, Vol. 61, pp. 3957–3968.

By: B. White*, E. Gökce n, A. Nepomuceno n, D. Muddiman n, T. Sanders* & J. Davis*

author keywords: IgE binding; LC-MS/MS; peanut; proteomics; skins; testae
MeSH headings : Allergens / immunology; Antigens, Plant / immunology; Arachis / chemistry; Arachis / immunology; Humans; Immunoglobulin E / metabolism; Peanut Hypersensitivity / immunology; Plant Proteins / analysis; Plant Proteins / chemistry; Plant Proteins / immunology; Proteomics; Seeds / chemistry; Seeds / immunology
topics (OpenAlex): Food Allergy and Anaphylaxis Research; Allergic Rhinitis and Sensitization; Proteins in Food Systems
TL;DR: Findings indicate that peanut skins contain potentially allergenic proteins; however, the presence of phenolic compounds may attenuate this effect. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Factorial Experimental Designs Elucidate Significant Variables Affecting Data Acquisition on a Quadrupole Orbitrap Mass Spectrometer

Randall, S. M., Cardasis, H. L., & Muddiman, D. C. (2013, August 2). Journal of the American Society for Mass Spectrometry, Vol. 24, pp. 1501–1512.

By: S. Randall n, H. Cardasis* & D. Muddiman n

author keywords: Design of experiments; Data dependent acquisition; Proteome coverage; Q Exactive; FTMS
MeSH headings : Models, Chemical; Proteome / analysis; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT): A Novel Glycan-Relative Quantification Strategy

Walker, S. H., Taylor, A. D., & Muddiman, D. C. (2013, July 15). Journal of the American Society for Mass Spectrometry, Vol. 24, pp. 1376–1384.

By: S. Walker n, A. Taylor n & D. Muddiman n

author keywords: Glycomics; Hydrazide Reagents; Stable Isotope Labeling; Normalization; Hydrazone; Formation; Relative Quantification; N-Linked Glycans
MeSH headings : Chromatography, Reverse-Phase; Glycomics / methods; Glycoproteins / chemistry; Glycosylation; Humans; Isotope Labeling / methods; Mass Spectrometry / methods; Plasma / chemistry; Polysaccharides / analysis; Polysaccharides / isolation & purification
topics (OpenAlex): Glycosylation and Glycoproteins Research; Advanced Proteomics Techniques and Applications; Enzyme Structure and Function
TL;DR: The ability to spike in a glycoprotein and detect change at the glycan level validates both the sample preparation and data analysis strategy, making INLIGHT an invaluable relative quantification strategy for the field of glycomics. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

MSiReader: An Open-Source Interface to View and Analyze High Resolving Power MS Imaging Files on Matlab Platform

Robichaud, G., Garrard, K. P., Barry, J. A., & Muddiman, D. C. (2013, March 27). Journal of the American Society for Mass Spectrometry, Vol. 24, pp. 718–721.

By: G. Robichaud n, K. Garrard n, J. Barry n & D. Muddiman n

author keywords: Mass spectrometry imaging; Software; High mass resolving power; FT-ICR-MS; Open-source
MeSH headings : Algorithms; Computational Biology; Image Processing, Computer-Assisted / methods; Internet; Mass Spectrometry / methods; Software; User-Computer Interface
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Isotope Analysis in Ecology
TL;DR: MSiReader is introduced, a free open source application to read and analyze high resolution MSI data from the most common MSi data formats and is built on the Matlab platform and includes a large selection of data analysis tools and features. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Mass Recalibration of FT-ICR Mass Spectrometry Imaging Data Using the Average Frequency Shift of Ambient Ions

Barry, J. A., Robichaud, G., & Muddiman, D. C. (2013, May 28). Journal of the American Society for Mass Spectrometry, Vol. 24, pp. 1137–1145.

By: J. Barry n, G. Robichaud n & D. Muddiman n

author keywords: Mass spectrometry imaging; FT-ICR MS; Mass calibration; MALDESI; MMA
MeSH headings : Animals; Brain / anatomy & histology; Brain Chemistry; Calibration; Cyclotrons; Fourier Analysis; Infrared Rays; Ions; Mass Spectrometry / methods; Mass Spectrometry / statistics & numerical data; Mice; Molecular Imaging / methods; Molecular Imaging / statistics & numerical data; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Electrospray Ionization / statistics & numerical data; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / statistics & numerical data
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Advanced Proteomics Techniques and Applications
TL;DR: A calibration method is applied based on the observed cyclotron frequency shift of ions found in the ambient laboratory environment, polydimethylcyclosiloxanes (PDMS) to achieve part per billion mass accuracy with relatively high precision for an infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) MSI dataset. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Monolignol Pathway 4-Coumaric Acid:Coenzyme A Ligases in Populus. trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes 

Chen, H.-C., Song, J., Williams, C. M., Shuford, C. M., Liu, J., Wang, J. P., … Chiang, V. L. (2013, January 23). PLANT PHYSIOLOGY, Vol. 161, pp. 1501–1516.

By: H. Chen n, J. Song n, C. Williams n, C. Shuford n, J. Liu n, J. Wang n, Q. Li n, R. Shi n ...

Contributors: H. Chen n, J. Song n, C. Williams n, C. Shuford n, J. Liu n, J. Wang n, Q. Li n, R. Shi n ...

MeSH headings : Allosteric Regulation / drug effects; Binding Sites; Biosynthetic Pathways / drug effects; Blotting, Western; Caffeic Acids / pharmacology; Coenzyme A / metabolism; Coenzyme A Ligases / antagonists & inhibitors; Coenzyme A Ligases / metabolism; Computer Simulation; Coumaric Acids / chemistry; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Kinetics; Lignin / biosynthesis; Lignin / chemistry; Phenylpropionates / metabolism; Phosphoproteins / metabolism; Phosphorylation / drug effects; Plant Extracts; Populus / drug effects; Populus / enzymology; Propionates; Proteomics; Recombinant Fusion Proteins / metabolism; Sequence Homology, Amino Acid; Substrate Specificity / drug effects; Xylem / drug effects; Xylem / metabolism
topics (OpenAlex): Biochemical and biochemical processes; Plant biochemistry and biosynthesis; Plant Gene Expression Analysis
TL;DR: Evidence is presented of two monolignol 4CLs, Ptr4CL3 and P tr4CL5, in Populus trichocarpa that act to mitigate the inhibition by caffeic acid to maintain a proper ligation flux and coenzyme A flux with novel specificity. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Multi-peptide nLC-PC-IDMS-SRM-based Assay for the quantification of biomarkers in the chicken ovarian cancer model

Methods, 61(3), 323–330.

By: G. Andrews Kingon n, J. Petitte n, D. Muddiman n & A. Hawkridge*

author keywords: Proteomics; Quantification; Ovarian cancer; Chicken; Biomarkers
MeSH headings : Amino Acid Sequence; Animals; Biological Assay; Biomarkers, Tumor / analysis; Biomarkers, Tumor / blood; Biomarkers, Tumor / chemistry; Calibration; Carbon Isotopes; Chickens; Disease Models, Animal; Female; Humans; Indicator Dilution Techniques; Isotope Labeling; Molecular Sequence Data; Nitrogen Isotopes; Ovarian Neoplasms / chemistry; Ovarian Neoplasms / metabolism; Peptide Fragments / analysis; Prealbumin / analysis; Prealbumin / chemistry; Prealbumin / metabolism; Proteomics / methods; Proteomics / standards; Tandem Mass Spectrometry / methods; Tandem Mass Spectrometry / standards; Vitellogenins / analysis; Vitellogenins / blood; Vitellogenins / chemistry; alpha-Macroglobulins / analysis; alpha-Macroglobulins / chemistry; alpha-Macroglobulins / metabolism
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Advanced Biosensing Techniques and Applications; Monoclonal and Polyclonal Antibodies Research
TL;DR: These data illustrate the successes and challenges of qualifying quantitative levels of proteins from an in-gel digestion sample preparation followed by LC-MS/MS (GeLC) label-free discovery-based approach to a targeted SRM-based quantitative assay in plasma and tissues. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2013 article

Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa

Shi, R., Shuford, C. M., Wang, J. P., Sun, Y.-H., Yang, Z., Chen, H.-C., … Chiang, V. L. (2013, June 13). Planta, Vol. 238, pp. 487–497.

By: R. Shi n, C. Shuford n, J. Wang n, Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n ...

Contributors: R. Shi n, C. Shuford n, J. Wang n, Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n ...

MeSH headings : Gene Expression Regulation, Plant / physiology; Mass Spectrometry; Phenylalanine Ammonia-Lyase / metabolism; Populus / enzymology; Populus / metabolism
topics (OpenAlex): Plant Gene Expression Analysis; Plant biochemistry and biosynthesis; Horticultural and Viticultural Research
TL;DR: A greater functional significance of subgroup A PtrPALs for stem wood formation is indicated, and the role of gene post-transcriptional regulation is highlighted. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Temporal Analysis of theMagnaporthe OryzaeProteome During Conidial Germination and Cyclic AMP (cAMP)-mediated Appressorium Formation

Molecular & Cellular Proteomics, 12(8), 2249–2265.

By: W. Franck*, E. Gokce n, Y. Oh n, D. Muddiman n & R. Dean n

MeSH headings : Cyclic AMP / metabolism; Fungal Proteins / metabolism; Magnaporthe / growth & development; Magnaporthe / metabolism; Mitochondrial Proteins / metabolism; Peptide Hydrolases / metabolism; Proteome; Spores, Fungal / growth & development; Spores, Fungal / metabolism
topics (OpenAlex): Fungal and yeast genetics research; Mycotoxins in Agriculture and Food; Plant-Microbe Interactions and Immunity
TL;DR: The data presented here provide the first comprehensive view of the M. oryzae proteome during early infection-related development and highlight biological processes important for pathogenicity. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2013 article

The use of a xylosylated plant glycoprotein as an internal standard accounting for N‐linked glycan cleavage and sample preparation variability

Walker, S. H., Taylor, A. D., & Muddiman, D. C. (2013, May 7). Rapid Communications in Mass Spectrometry, Vol. 27, pp. 1354–1358.

By: S. Walker n, A. Taylor n & D. Muddiman n

MeSH headings : Analytic Sample Preparation Methods / standards; Glycoproteins / chemistry; Glycosylation; Mass Spectrometry / standards; Plant Proteins / chemistry; Polysaccharides / chemistry; Protein Processing, Post-Translational; Reference Standards
topics (OpenAlex): Glycosylation and Glycoproteins Research; Proteoglycans and glycosaminoglycans research; Genomics and Phylogenetic Studies
TL;DR: The inclusion of two internal standards, maltoheptaose and horseradish peroxidase (HRP), is studied to demonstrate the effectiveness of using a glycoprotein as an internal standard in glycan relative quantification experiments. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2013 article

Understanding the Role of Proteolytic Digestion on Discovery and Targeted Proteomic Measurements Using Liquid Chromatography Tandem Mass Spectrometry and Design of Experiments

Loziuk, P. L., Wang, J., Li, Q., Sederoff, R. R., Chiang, V. L., & Muddiman, D. C. (2013, October 22). Journal of Proteome Research, Vol. 12, pp. 5820–5829.

By: P. Loziuk n, J. Wang n, Q. Li n, R. Sederoff n, V. Chiang n & D. Muddiman n

Contributors: P. Loziuk n, J. Wang n, Q. Li n, R. Sederoff n, V. Chiang n & D. Muddiman n

author keywords: design of experiments; proteolytic digestion; LC-MS/MS; Populus trichocarpa
MeSH headings : Amino Acid Sequence; Animals; Cattle; Chromatography, Liquid / standards; Factor Analysis, Statistical; Hydrogen-Ion Concentration; Isotope Labeling; Molecular Sequence Annotation; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / isolation & purification; Plant Proteins / chemistry; Plant Proteins / isolation & purification; Populus / chemistry; Protein Stability; Proteolysis; Proteomics / methods; Proteomics / standards; Research Design; Swine; Tandem Mass Spectrometry / standards; Trypsin / chemistry; Xylem / chemistry
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The tryptic digest optimization described here has eliminated this previously observed peptide decay as well as provided a greater understanding and standardization for a common but critical sample treatment used across the field of proteomics. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Alexander R. Ivanov, Alexander V. Lazarev (Eds): Sample preparation in biological mass spectrometry

Analytical and Bioanalytical Chemistry, 404(5), 1331–1332.

By: D. Muddiman n

topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2012 article

Analytical strategies for the global quantification of intact proteins

Collier, T. S., & Muddiman, D. C. (2012, July 21). Amino Acids, Vol. 43, pp. 1109–1117.

By: T. Collier n & D. Muddiman n

author keywords: Proteomics; Intact proteins; Quantitation; Mass spectrometry
MeSH headings : Animals; Humans; Mass Spectrometry; Proteome / chemistry; Proteome / isolation & purification; Proteome / metabolism; Proteomics; Staining and Labeling; Two-Dimensional Difference Gel Electrophoresis
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: Techniques contained in this review include the more traditional and widely employed methodology of differential gel electrophoresis and more recently developed mass spectrometry-based techniques including metabolic labeling, chemical labeling, and label-free methodologies. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Comprehensive Quantification of Monolignol-Pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry

Journal of Proteome Research, 11(6), 3390–3404.

By: C. Shuford n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n, R. Shi n, R. Sederoff n, V. Chiang n, D. Muddiman n

Contributors: C. Shuford n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n, R. Shi n, R. Sederoff n, V. Chiang n, D. Muddiman n

author keywords: quantification; isotope dilution mass spectrometry; selected reaction monitoring; filter-aided sample preparation; monolignol biosynthesis
MeSH headings : Amino Acid Sequence; Biosynthetic Pathways; Isotope Labeling; Molecular Sequence Data; Plant Proteins / chemistry; Plant Proteins / metabolism; Populus / enzymology; Proteome / chemistry; Proteome / metabolism; Reference Standards; Spectrometry, Mass, Electrospray Ionization / standards; Xylem / enzymology
topics (OpenAlex): Fermentation and Sensory Analysis; Plant Gene Expression Analysis; Biochemical and biochemical processes
TL;DR: This represents the most comprehensive assay for quantification of proteins regulating monolignol biosynthesis and will lead to a better understanding of lignin formation at a systems level. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science, ORCID
Added: August 6, 2018

2012 article

Coordination-Driven Self-Assembly of Charged and Neutral Dendritic Tetrakis(ferrocenyl) Rhomboids

Li, Q.-J., Zhao, G.-Z., Chen, L.-J., Tan, H., Wang, C.-H., Wang, D.-X., … Yang, H.-B. (2012, October 3). Organometallics, Vol. 31, pp. 7241–7247.

By: Q. Li*, G. Zhao*, L. Chen*, H. Tan*, C. Wang*, D. Wang*, D. Lehman n, D. Muddiman n, H. Yang*

topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Supramolecular Chemistry and Complexes; Metal-Organic Frameworks: Synthesis and Applications
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

Direct Analysis of Textile Fabrics and Dyes Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry

Cochran, K. H., Barry, J. A., Muddiman, D. C., & Hinks, D. (2012, December 13). Analytical Chemistry, Vol. 85, pp. 831–836.

By: K. Cochran n, J. Barry n, D. Muddiman n & D. Hinks n

MeSH headings : Coloring Agents / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Textiles / analysis
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Cultural Heritage Materials Analysis; Isotope Analysis in Ecology
TL;DR: A direct analysis of dyed textile fabric was performed using the infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source for MS, allowing the omission of the lengthy sample preparation involved in extraction, thereby significantly reducing the overall analysis time. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

Evidence for Complex Molecular Architectures for Solvent-Extracted Lignins

Harton, S. E., Pingali, S. V., Nunnery, G. A., Baker, D. A., Walker, S. H., Muddiman, D. C., … Langan, P. (2012, April 17). ACS Macro Letters, Vol. 1, pp. 568–573.

By: S. Harton, S. Pingali, G. Nunnery, D. Baker*, S. Walker n, D. Muddiman n, T. Koga*, T. Rials*, V. Urban, P. Langan

topics (OpenAlex): Lignin and Wood Chemistry; Fermentation and Sensory Analysis; Biofuel production and bioconversion
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC–MS/MS based protein quantification and metabolic flux analysis

Wang, J. P., Shuford, C. M., Li, Q., Song, J., Lin, Y.-C., Sun, Y.-H., … Chiang, V. L. (2012, May 24). Planta, Vol. 236, p. 795–808.

By: J. Wang n, C. Shuford n, Q. Li n, J. Song n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n ...

Contributors: J. Wang n, C. Shuford n, Q. Li n, J. Song n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n ...

author keywords: Lignin; Syringyl lignin; Angiosperm; Kinetics; Subcellular localization
MeSH headings : Cloning, Molecular; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hydroxylation; Kinetics; Lignin / analysis; Lignin / biosynthesis; Mixed Function Oxygenases / metabolism; Plants, Genetically Modified; Populus / metabolism; Xylem / enzymology; Yeasts / metabolism
topics (OpenAlex): Plant Gene Expression Analysis; Fermentation and Sensory Analysis; Biochemical and biochemical processes
TL;DR: The combination of genetic analysis, absolute protein quantitation-based enzyme kinetics, homologous CPR specificity, SNP characterization, and ER localization provides a more rigorous basis for a comprehensive systems understanding of 5-hydroxylation in lignin biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2012 article

High-resolution mass spectrometry

Maurer, H. H., & Muddiman, D. C. (2012, April 9). Analytical and Bioanalytical Chemistry, Vol. 403, pp. 1201–1202.

By: H. Maurer* & D. Muddiman n

MeSH headings : Animals; Biological Science Disciplines; Environmental Pollutants / analysis; Food Analysis; Humans; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Substance Abuse Detection
topics (OpenAlex): Metabolomics and Mass Spectrometry Studies; Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: An overview of the current use of high-resolution mass spectrometry in the life sciences is given, intended for all readers with an interest in application of HRMS in clinical and forensic toxicology, doping control, drug metabolism, food analysis, environmental sciences, and proteomics and related fields. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

In-Depth Analysis of the Magnaporthe oryzae Conidial Proteome

Gokce, E., Franck, W. L., Oh, Y., Dean, R. A., & Muddiman, D. C. (2012, October 8). Journal of Proteome Research, Vol. 11, pp. 5827–5835.

By: E. Gokce n, W. Franck n, Y. Oh n, R. Dean n & D. Muddiman n

author keywords: proteomics; mass spectrometry; Magnaporthe oryzae; FASP; statistical tests; differential protein expression
MeSH headings : Cell Nucleus / chemistry; Chemical Fractionation / methods; Chromatography, Liquid / methods; Cytoplasm / chemistry; Fungal Proteins / analysis; Fungal Proteins / chemistry; Magnaporthe / chemistry; Mass Spectrometry / methods; Nanotechnology; Nuclear Proteins / analysis; Nuclear Proteins / chemistry; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spores, Fungal / chemistry; Time Factors
topics (OpenAlex): Fungal and yeast genetics research; Mycotoxins in Agriculture and Food; Advanced Proteomics Techniques and Applications
TL;DR: The identification of 2912 conidial proteins at a 1% protein false discovery rate (FDR) is reported and the most extensive study performed on M. oryzae conidia to date is presented, suggesting the detected proteins build a representative portion of the predicted proteome. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Imaging Source Coupled to a FT-ICR Mass Spectrometer

Robichaud, G., Barry, J. A., Garrard, K. P., & Muddiman, D. C. (2012, December 4). Journal of the American Society for Mass Spectrometry, Vol. 24, pp. 92–100.

By: G. Robichaud n, J. Barry n, K. Garrard n & D. Muddiman n

author keywords: FTMS; Mass spectrometry imaging; Infrared laser; MALDESI; Atmospheric pressure
MeSH headings : Animals; Atmospheric Pressure; Brain Chemistry; Fourier Analysis; Lipids / analysis; Lipids / chemistry; Lung / chemistry; Mice; Molecular Imaging / methods; Myocardium / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Ion-surface interactions and analysis
TL;DR: This work introduces here a versatile source for MALDESI imaging analysis coupled to a hybrid LTQ-FT-ICR mass spectrometer, offering single shot or multi-shot capability per pixel with full control over the laser repetition rate and mass Spectrometer scanning cycle. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Peptide Production and Decay Rates Affect the Quantitative Accuracy of Protein Cleavage Isotope Dilution Mass Spectrometry (PC-IDMS)

Molecular & Cellular Proteomics, 11(9), 814–823.

By: C. Shuford n, R. Sederoff n, V. Chiang n & D. Muddiman n

MeSH headings : Indicator Dilution Techniques; Isotope Labeling; Mass Spectrometry; Peptide Biosynthesis; Peptides / analysis; Peptides / chemistry; Peptides / metabolism; Populus / enzymology; Populus / metabolism; Proteolysis; Xylem / enzymology
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Molecular Biology Techniques and Applications
TL;DR: It is concluded that the instability of peptides can often significantly bias the protein quantities measured in protein cleavage isotope dilution mass spectrometry-based assays and suggest peptide stability be made a priority when selecting peptides to use for quantification. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

ACS Chemical Biology, 8(1), 200–208.

By: I. Koryakina n, J. McArthur*, S. Randall, M. Draelos*, E. Musiol*, D. Muddiman*, T. Weber*, G. Williams*

MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Chromatography, Liquid; Coenzyme A Ligases / chemistry; Coenzyme A Ligases / genetics; Crystallography, X-Ray; Genetic Variation; Models, Molecular; Substrate Specificity
topics (OpenAlex): Microbial Natural Products and Biosynthesis; Plant biochemistry and biosynthesis; Genomics and Phylogenetic Studies
TL;DR: Results reveal that polyketide biosynthetic machinery might be more tolerant to non-natural substrates than previously established, and that mutant synthetases are valuable tools for probing the specificity of biosynthetics machinery. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries, Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae

PLoS ONE, 7(8), e42868.

By: Y. Oh n, W. Franck n, S. Han*, A. Shows n, E. Gokce n, D. Muddiman n, R. Dean n

Ed(s): K. Nielsen

MeSH headings : Boronic Acids / pharmacology; Bortezomib; Cluster Analysis; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Germination / genetics; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Mutation; Nitrogen / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Polyubiquitin / genetics; Polyubiquitin / metabolism; Proteasome Inhibitors / pharmacology; Proteolysis / drug effects; Proteomics; Pyrazines / pharmacology; Ubiquitination
topics (OpenAlex): Fungal and yeast genetics research; Ubiquitin and proteasome pathways; Microbial Natural Products and Biosynthesis
TL;DR: This study suggests that ubiquitination of target proteins plays an important role in nutrient assimilation, development and pathogenicity of M. oryzae. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2012 article

Systematic Comparison of Reverse Phase and Hydrophilic Interaction Liquid Chromatography Platforms for the Analysis of N-Linked Glycans

Walker, S. H., Carlisle, B. C., & Muddiman, D. C. (2012, September 6). Analytical Chemistry, Vol. 84, pp. 8198–8206.

By: S. Walker n, B. Carlisle n & D. Muddiman n

MeSH headings : Chromatography, Reverse-Phase; Hydrophobic and Hydrophilic Interactions; Mass Spectrometry; Polysaccharides / analysis
topics (OpenAlex): Glycosylation and Glycoproteins Research; Analytical Chemistry and Chromatography; Chromatography in Natural Products
TL;DR: The data presented show that separation of derivatized N-linked glycans by reverse phase chromatography significantly out-performs traditional separation of native or derivatization glycansBy hydrophilic interaction chromatography. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells

Sarkar, P., Randall, S. M., Muddiman, D. C., & Rao, B. M. (2012, September 15). Molecular & Cellular Proteomics, Vol. 11, pp. 1829–1839.

By: P. Sarkar n, S. Randall n, D. Muddiman n & B. Rao n

Contributors: P. Sarkar n, S. Randall n, D. Muddiman n & B. Rao n

MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned / analysis; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Mice; Proteins / analysis; Secretory Pathway; Subcellular Fractions / chemistry
topics (OpenAlex): 3D Printing in Biomedical Research; Pluripotent Stem Cells Research; Advanced Proteomics Techniques and Applications
TL;DR: Targeted proteomics of secretory pathway organelles is shown to be a powerful alternate approach for interrogating the cellular secretome and expected to be particularly valuable in two contexts highly relevant to hESC biology: obtaining a temporal snapshot of proteins secreted in response to a differentiation trigger, and identifying proteinssecreted by cells that are isolated from a heterogeneous population. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Absolute quantification of free glutathione and cysteine in aquatic insects using isotope dilution and selected reaction monitoring

Analytical and Bioanalytical Chemistry, 402(1), 357–366.

By: C. Shuford n, M. Poteat n, D. Buchwalter n & D. Muddiman n

Contributors: C. Shuford n, M. Poteat n, D. Buchwalter n & D. Muddiman n

author keywords: Glutathione; Cysteine; Selected reaction monitoring; Absolute quantification; Isotope dilution
MeSH headings : Animals; Aquatic Organisms / chemistry; Carbon Isotopes / analysis; Chromatography, High Pressure Liquid / methods; Cysteine / analysis; Glutathione / analysis; Indicator Dilution Techniques; Insecta / chemistry; Nitrogen Isotopes / analysis
topics (OpenAlex): Environmental Toxicology and Ecotoxicology; Parasite Biology and Host Interactions; Sulfur Compounds in Biology
TL;DR: A simple and robust isotope dilution mass spectrometry-based assay was developed for the determination of free cysteine and glutathione in aquatic insects and was demonstrated to have a quantitative dynamic range spanning nearly three orders of magnitude. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2011 article

Capitalizing on the hydrophobic bias of electrospray ionization through chemical modification in mass spectrometry-based proteomics

Shuford, C. M., & Muddiman, D. C. (2011, June 1). Expert Review of Proteomics, Vol. 8, pp. 317–323.

By: C. Shuford n & D. Muddiman n

author keywords: ALiPHAT; electrospray ionization; hydrophobic tagging; mass spectrometry; quantification
MeSH headings : Humans; Hydrophobic and Hydrophilic Interactions; Proteomics / methods; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: Improvements in targeted, mass spectrometry-based proteomic strategies are achieved by taking advantage of the mechanism of ESI through the use of hydrophobic chemical derivatization. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Comparison of stable‐isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

Collier, T. S., Randall, S. M., Sarkar, P., Rao, B. M., Dean, R. A., & Muddiman, D. C. (2011, August 4). Rapid Communications in Mass Spectrometry, Vol. 25, pp. 2524–2532.

By: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n, R. Dean n & D. Muddiman n

Contributors: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 article

Evaluation of Normalization Methods on GeLC-MS/MS Label-Free Spectral Counting Data to Correct for Variation during Proteomic Workflows

Gokce, E., Shuford, C. M., Franck, W. L., Dean, R. A., & Muddiman, D. C. (2011, September 23). Journal of the American Society for Mass Spectrometry, Vol. 22, pp. 2199–2208.

By: E. Gokce n, C. Shuford n, W. Franck n, R. Dean n & D. Muddiman n

author keywords: Label-free; Proteomics; Mass spectrometry; Total spectral count normalization; Normalized spectral abundance factor; Quantification; Spectral counting; Significance test; Fold-change; GeLC-MS/MS; nanoLC-MS; Orbitrap XL
MeSH headings : Animals; Chickens; Chromatography, Gel / methods; Fungal Proteins / analysis; Fungal Proteins / chemistry; Horses; Myoglobin / analysis; Myoglobin / chemistry; Ovalbumin / analysis; Ovalbumin / chemistry; Peptide Mapping / methods; Proteomics / methods; Proteomics / standards; Regression Analysis; Reproducibility of Results; Tandem Mass Spectrometry / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The correlation between SpCs of the same proteins across the different data sets was investigated and it was reported that TSpC normalization and NSAF normalization yielded almost ideal slopes of unity for normalized SpC versus average normalized SpCs, while NSP did not afford effective corrections of the unnormalized data. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Facile Self-Assembly of Supramolecular Hexakisferrocenyl Triangles via Coordination-Driven Self-Assembly and Their Electrochemical Behavior

Zhao, G.-Z., Li, Q.-J., Chen, L.-J., Tan, H., Wang, C.-H., Lehman, D. A., … Yang, H.-B. (2011, June 7). Organometallics, Vol. 30, pp. 3637–3642.

topics (OpenAlex): Ferrocene Chemistry and Applications; Dendrimers and Hyperbranched Polymers; Supramolecular Chemistry and Complexes
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Global optimization of the infrared matrix‐assisted laser desorption electrospray ionization (IR MALDESI) source for mass spectrometry using statistical design of experiments

Barry, J. A., & Muddiman, D. C. (2011, November 8). Rapid Communications in Mass Spectrometry, Vol. 25, pp. 3527–3536.

By: J. Barry n & D. Muddiman n

MeSH headings : Animals; Cattle; Cytochromes c / analysis; Cytochromes c / chemistry; Horses; Limit of Detection; Research Design; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: The use of statistical DOE is described to improve a few of the analytical figures of merit of the infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source for mass spectrometry. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Hydrophobic Derivatization of N-linked Glycans for Increased Ion Abundance in Electrospray Ionization Mass Spectrometry

Walker, S. H., Lilley, L. M., Enamorado, M. F., Comins, D. L., & Muddiman, D. C. (2011, May 2). Journal of the American Society for Mass Spectrometry, Vol. 22, pp. 1309–1317.

By: S. Walker n, L. Lilley*, M. Enamorado n, D. Comins n & D. Muddiman n

author keywords: Hydrophobic Derivatization; HILIC; nanoESI; N-Linked Glycans; Hydrazone Formation
MeSH headings : Blood Proteins / chemistry; Carbohydrate Sequence; Chromatography, Liquid; Glycoproteins / chemistry; Humans; Hydrazines / chemistry; Hydrophobic and Hydrophilic Interactions; Polysaccharides / chemistry; Small Molecule Libraries; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Mass Spectrometry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Glycosylation and Glycoproteins Research
TL;DR: The results of this study will direct the future synthesis of hydrophobic reagents for glycan analysis using the correlation between hydrophobicity and theoretical non-polar surface area calculation to facilitate the development of an optimum tag for gly can derivatization. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Identification of alternative splice variants in Aspergillus flavus through comparison of multiple tandem MS search algorithms

BMC Genomics, 12.

By: K. Chang & D. Muddiman

Source: NC State University Libraries
Added: August 6, 2018

2011 article

Improving Proteome Coverage on a LTQ-Orbitrap Using Design of Experiments

Andrews, G. L., Dean, R. A., Hawkridge, A. M., & Muddiman, D. C. (2011, February 14). Journal of the American Society for Mass Spectrometry, Vol. 22, pp. 773–783.

By: G. Andrews n, R. Dean n, A. Hawkridge n & D. Muddiman n

author keywords: LTQ-Orbitrap XL MS/MS instrument parameters; Design of experiments; Fractional-factorial design; Saccharomyces cerevisiae; Proteomics
MeSH headings : Peptide Fragments; Peptide Mapping; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods; Temperature
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Advanced Biosensing Techniques and Applications
TL;DR: The DOE approach represents a time- and cost-effective method for empirically optimizing MS-based proteomics workflows including sample preparation, LC conditions, and multiple instrument platforms. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Increasing proteome coverage with offline RP HPLC coupled to online RP nanoLC–MS

Gokce, E., Andrews, G. L., Dean, R. A., & Muddiman, D. C. (2011, February 5). Journal of Chromatography B, Vol. 879, pp. 610–614.

By: E. Gokce n, G. Andrews n, R. Dean n & D. Muddiman n

author keywords: RP HPLC; Fractionation methods; Proteomics; Mass spectrometry
MeSH headings : Chromatography, High Pressure Liquid / methods; Chromatography, Reverse-Phase / methods; Mass Spectrometry / methods; Nanotechnology; Peptide Fragments / analysis; Peptide Fragments / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / metabolism
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The success of performing offline reversed phase high pressure liquid chromatography (HPLC) fractionation on a C18 2.0 mm×150 mm column at the peptide level with microliter per minute flow rates prior to online nano-flow reversed phase liquid Chromatography mass spectrometry (nanoLC-MS) using the well-studied fungus Saccharomyces cerevisiae is reported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

John B. Fenn special issue recognizing the science of Professor John B. Fenn foreword

International Journal of Mass Spectrometry, 300(2-3), 72–73.

By: M. El-Shall & D. Muddiman

Source: NC State University Libraries
Added: August 6, 2018

2011 article

John Bennett Fenn (1917–2010)

Muddiman, D. C. (2011, January 13). Science, Vol. 331, pp. 160–160.

By: D. Muddiman n

MeSH headings : Chemistry / history; History, 20th Century; History, 21st Century; Nobel Prize; Spectrometry, Mass, Electrospray Ionization / history; United States
topics (OpenAlex): Various Chemistry Research Topics
TL;DR: The Nobel Prize in Chemistry did not define John Fenn, nor did it change him; he was the same man before and after, always showing a strong sense of humility. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis

Chen, H.-C., Li, Q., Shuford, C. M., Liu, J., Muddiman, D. C., Sederoff, R. R., & Chiang, V. L. (2011, December 12). Proceedings of the National Academy of Sciences, Vol. 108, pp. 21253–21258.

By: H. Chen*, Q. Li*, C. Shuford n, J. Liu, D. Muddiman n, R. Sederoff*, V. Chiang*

MeSH headings : Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / metabolism; Chromatography, Liquid; Coumaric Acids; DNA Primers / genetics; Hydroxylation; Immunoprecipitation; Kinetics; Lignin / biosynthesis; Mass Spectrometry; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Microscopy, Confocal; Molecular Structure; Multiprotein Complexes / chemistry; Multiprotein Complexes / metabolism; Phenols; Phenylpropionates; Plasmids / genetics; Populus / metabolism; Propionates; Trans-Cinnamate 4-Monooxygenase / chemistry; Trans-Cinnamate 4-Monooxygenase / metabolism; Xylem / metabolism; Yeasts
topics (OpenAlex): Plant Gene Expression Analysis; Microbial metabolism and enzyme function; Carbohydrate Chemistry and Synthesis
TL;DR: Affinity purification-quantitative mass spectrometry, bimolecular fluorescence complementation, chemical cross-linking, and reciprocal coimmunoprecipitation provide further evidence for functional involvement of membrane protein complexes in monolignol biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 chapter

N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry

In Methods in Molecular Biology (pp. 87–97).

By: M. Bereman* & D. Muddiman n

author keywords: N-linked glycans; NanoLC mass spectrometry; Glycoproteins; Glycosylation; Glycan profiling; Hydrophilic interaction chromatography; Glycobiology; Glycan algorithms
MeSH headings : Acetylglucosamine / metabolism; Asparagine; Blood Proteins / chemistry; Blood Proteins / metabolism; Chromatography, Liquid / methods; Glycoproteins / metabolism; Glycoside Hydrolases / metabolism; Hydrophobic and Hydrophilic Interactions; Mannose / metabolism; Mass Spectrometry / methods; Nanotechnology / methods; Nitrogen / chemistry; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism; Polysaccharides / analysis; Polysaccharides / chemistry; Polysaccharides / isolation & purification; Polysaccharides / metabolism; Protein Denaturation; Solid Phase Extraction; Substrate Specificity; Tandem Mass Spectrometry
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Analytical Chemistry and Chromatography
TL;DR: Material, methods, and algorithms for the identification of both glycan composition and structure are summarized and potential problems and their resolutions are addressed. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2011 article

Performance Characteristics of a New Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer (TripleTOF 5600)

Andrews, G. L., Simons, B. L., Young, J. B., Hawkridge, A. M., & Muddiman, D. C. (2011, May 27). Analytical Chemistry, Vol. 83, pp. 5442–5446.

By: G. Andrews n, B. Simons, J. Young, A. Hawkridge n & D. Muddiman n

MeSH headings : Fungal Proteins / metabolism; Proteome; Saccharomyces cerevisiae / metabolism; Tandem Mass Spectrometry / instrumentation; Trypsin / metabolism
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Spectral Accuracy and Sulfur Counting Capabilities of the LTQ-FT-ICR and the LTQ-Orbitrap XL for Small Molecule Analysis

Blake, S. L., Walker, S. H., Muddiman, D. C., Hinks, D., & Beck, K. R. (2011, September 20). Journal of the American Society for Mass Spectrometry, Vol. 22, pp. 2269–2275.

By: S. Blake n, S. Walker n, D. Muddiman n, D. Hinks n & K. Beck n

author keywords: Electrospray ionization; FT-ICR-MS; Orbitrap-MS; Forensic analysis; Textiles; Trace evidence
MeSH headings : Chi-Square Distribution; Coloring Agents / chemistry; Forensic Sciences; Fourier Analysis; Mass Spectrometry / methods; Polyesters / chemistry; Sensitivity and Specificity; Sulfur / analysis; Textiles / analysis
topics (OpenAlex): Isotope Analysis in Ecology; Identification and Quantification in Food; Cultural Heritage Materials Analysis
TL;DR: DY42, a high-volume disperse dye for polyester, was used to compare the capabilities of the LTQ-Orbitrap XL and theLTQ-FT-ICR with respect to mass measurement accuracy (MMA), spectral accuracy, and sulfur counting. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Stable-Isotope Labeled Hydrophobic Hydrazide Reagents for the Relative Quantification of N-Linked Glycans by Electrospray Ionization Mass Spectrometry

Walker, S. H., Budhathoki-Uprety, J., Novak, B. M., & Muddiman, D. C. (2011, July 20). Analytical Chemistry, Vol. 83, pp. 6738–6745.

By: S. Walker n, J. Budhathoki-Uprety n, B. Novak n & D. Muddiman n

MeSH headings : Carbon Isotopes / chemistry; Glycopeptides / analysis; Glycosylation; Hydrazones / chemistry; Hydrophobic and Hydrophilic Interactions; Isotope Labeling; Polysaccharides / analysis; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Glycosylation and Glycoproteins Research; Advanced Proteomics Techniques and Applications
TL;DR: It is shown that these reagents are capable of quantifying changes in glycosylation in simple mixtures, and the analytical variability of the reagents in pooled plasma samples is shown to be less than ±30%. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 article

The subcellular proteome of undifferentiated human embryonic stem cells

Sarkar, P., Collier, T. S., Randall, S. M., Muddiman, D. C., & Rao, B. M. (2011, December 5). PROTEOMICS, Vol. 12, pp. 421–430.

By: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n & B. Rao n

Contributors: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n & B. Rao n

author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
topics (OpenAlex): Cellular transport and secretion; CRISPR and Genetic Engineering; Advanced biosensing and bioanalysis techniques
TL;DR: The results show that systematic subcellular proteome analysis is a valuable tool for studying hESC biology and shows that proteomic analysis of the endoplasmic reticulum and Golgi compartments is a powerful alternative approach to identify secreted proteins since these are synthesized in the ER and transit through the Golgi. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

Binding site on human immunoglobulin G for the affinity ligand HWRGWV

Journal of Molecular Recognition, n/a-n/a.

By: H. Yang n, P. Gurgel n, D. Williams n, B. Bobay n, J. Cavanagh n, D. Muddiman n, R. Carbonell n

author keywords: hexamer ligand; immunoglobulin G; glycosylation; mass spectrometry; computational docking; binding site
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Endopeptidases / metabolism; Humans; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / chemistry; Immunoglobulin G / metabolism; Ligands; Models, Molecular; Molecular Sequence Data; Nerve Tissue Proteins / metabolism; Oligopeptides / chemistry; Oligopeptides / genetics; Oligopeptides / metabolism; Pepsin A / metabolism; Protein Binding; Protein Structure, Tertiary; Sequence Alignment; Staphylococcal Protein A / metabolism
topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Blood groups and transfusion
TL;DR: The results support the fact that a peptide as small as a hexamer can have specific interactions with large proteins such as hIgG and the HWRGWV ligand. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2010 journal article

Binding site on human immunoglobulin G for the affinity ligand HWRGWV

Journal of Molecular Recognition, 23(3), 271–282.

Source: NC State University Libraries
Added: August 6, 2018

2010 article

Design, modeling, fabrication, and evaluation of the air amplifier for improved detection of biomolecules by electrospray ionization mass spectrometry

Robichaud, G., Dixon, R. B., Potturi, A. S., Cassidy, D., Edwards, J. R., Sohn, A., … Muddiman, D. C. (2010, April 22). International Journal of Mass Spectrometry, Vol. 300, pp. 99–107.

By: G. Robichaud n, R. Dixon n, A. Potturi n, D. Cassidy n, J. Edwards n, A. Sohn n, T. Dow n, D. Muddiman n

author keywords: Air amplifier; Electrospray ionization; Modeling; Detection limits
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Electrohydrodynamics and Fluid Dynamics; Microfluidic and Capillary Electrophoresis Applications
TL;DR: A computer simulation that accurately portrays actual conditions and the results from these simulations are corroborated by the experimental data is developed, which can be used to predict outcomes from future designs resulting in a design process that is efficient in terms of financial cost and time. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Detection of Alternative Splice Variants at the Proteome Level inAspergillus flavus

Detection of Alternative Splice Variants at the Proteome Level in Aspergillus flavus. Journal of Proteome Research, 9(3), 1209–1217.

By: K. Chang n, D. Georgianna n, S. Heber n, G. Payne n & D. Muddiman n

author keywords: mass spectrometry; proteomics; alternative splicing; database; Aspergillus flavus
MeSH headings : Alternative Splicing; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Computer Simulation; Cytochrome-B(5) Reductase / chemistry; Cytochrome-B(5) Reductase / genetics; Databases, Protein; Fungal Proteins / biosynthesis; Fungal Proteins / chemistry; Fungal Proteins / genetics; Isotope Labeling; Mass Spectrometry; Models, Genetic; Protein Isoforms / biosynthesis; Protein Isoforms / chemistry; Protein Isoforms / genetics; Proteomics; Pyruvate Carboxylase / chemistry; Pyruvate Carboxylase / genetics; RNA Splice Sites; Reproducibility of Results
topics (OpenAlex): Advanced Proteomics Techniques and Applications; RNA and protein synthesis mechanisms; Genomics and Phylogenetic Studies
TL;DR: The introduction of an alternative splicing database helps identify more proteins and unveils more information about a proteome, and shows potential to improve genome annotation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: December 27, 2020

2010 article

Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics

Collier, T. S., Sarkar, P., Franck, W. L., Rao, B. M., Dean, R. A., & Muddiman, D. C. (2010, September 16). Analytical Chemistry, Vol. 82, pp. 8696–8702.

By: T. Collier n, P. Sarkar n, W. Franck n, B. Rao n, R. Dean n & D. Muddiman n

Contributors: T. Collier n, P. Sarkar n, W. Franck n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Erratum to: Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma

Hawkridge, A. M., Wysocky, R. B., Petitte, J. N., Anderson, K. E., Mozdziak, P. E., Fletcher, O. J., … Muddiman, D. C. (2010, August 13). Analytical and Bioanalytical Chemistry, Vol. 398, pp. 1835–1835.

By: A. Hawkridge n, R. Wysocky n, J. Petitte n, K. Anderson n, P. Mozdziak n, O. Fletcher n, J. Horowitz n, D. Muddiman n

Contributors: A. Hawkridge n, R. Wysocky n, J. Petitte n, K. Anderson n, P. Mozdziak n, O. Fletcher n, J. Horowitz n, D. Muddiman n

topics (OpenAlex): Advanced Proteomics Techniques and Applications
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Erratum to: Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2

Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, August 14). Analytical and Bioanalytical Chemistry, Vol. 398, pp. 1837–1837.

By: G. Andrews n, D. Lewis n, J. Notey n, R. Kelly n & D. Muddiman n

topics (OpenAlex): Advanced Proteomics Techniques and Applications; S100 Proteins and Annexins; Enzyme Structure and Function
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Erratum to: Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting

Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, August 12). Analytical and Bioanalytical Chemistry, Vol. 398, pp. 1839–1839.

By: G. Andrews n, D. Lewis n, J. Notey n, R. Kelly n & D. Muddiman n

topics (OpenAlex): Advanced Proteomics Techniques and Applications; Machine Learning in Bioinformatics; Genomics and Phylogenetic Studies
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Facile Self-Assembly of Dendritic Multiferrocenyl Hexagons and Their Electrochemistry

Zhao, G.-Z., Chen, L.-J., Wang, C.-H., Yang, H.-B., Ghosh, K., Zheng, Y.-R., … Stang, P. J. (2010, October 12). Organometallics, Vol. 29, pp. 6137–6140.

By: G. Zhao*, L. Chen*, C. Wang*, H. Yang*, K. Ghosh*, Y. Zheng*, M. Lyndon n, D. Muddiman n, P. Stang*

topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Ferrocene Chemistry and Applications; Molecular Junctions and Nanostructures
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Foreword

International Journal of Mass Spectrometry, 300(2-3), 72–73.

By: M. Samy El-Shall* & D. Muddiman n

topics (OpenAlex):
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2010 article

Interplay of Permanent Charge and Hydrophobicity in the Electrospray Ionization of Glycans

Walker, S. H., Papas, B. N., Comins, D. L., & Muddiman, D. C. (2010, June 30). Analytical Chemistry, Vol. 82, pp. 6636–6642.

By: S. Walker n, B. Papas n, D. Comins n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: The effectiveness of the incorporation of a permanent charge is studied and determined to hamper glycan analysis and a procedure for glycan hydrazone formation is optimized and outlined where a large number of variables were simultaneously analyzed using a fractional factorial design (FFD). (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma

Hawkridge, A. M., Wysocky, R. B., Petitte, J. N., Anderson, K. E., Mozdziak, P. E., Fletcher, O. J., … Muddiman, D. C. (2010, July 16). Analytical and Bioanalytical Chemistry, Vol. 398, pp. 737–749.

By: A. Hawkridge n, R. Wysocky n, J. Petitte n, K. Anderson n, P. Mozdziak n, O. Fletcher n, J. Horowitz n, D. Muddiman n

Contributors: A. Hawkridge n, R. Wysocky n, J. Petitte n, K. Anderson n, P. Mozdziak n, O. Fletcher n, J. Horowitz n, D. Muddiman n

author keywords: Genomics/proteomics; Mass spectrometry/ICP-MS; Biological samples; Bioanalytical methods; Clinical/biomedical analysis
MeSH headings : Adenocarcinoma / metabolism; Adenocarcinoma / pathology; Animals; Chickens; Female; Humans; Mass Spectrometry / methods; Ovarian Neoplasms / metabolism; Ovarian Neoplasms / pathology; Plasma / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Molecular Biology Techniques and Applications; Animal Virus Infections Studies
TL;DR: An experimental strategy for biomarker discovery is reported herein that combines the chicken model of EOC, longitudinal plasma sample collection with matched tissues, advanced mass spectrometry-based proteomics, and concepts derived from the index of individuality in an effort to identify diagnostic protein biomarkers. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

One-year plasma N-linked glycome intra-individual and inter-individual variability in the chicken model of spontaneous ovarian adenocarcinoma

Dixon, R. B., Bereman, M. S., Petitte, J. N., Hawkridge, A. M., & Muddiman, D. C. (2010, May 28). International Journal of Mass Spectrometry, Vol. 305, pp. 79–86.

By: R. Dixon n, M. Bereman n, J. Petitte n, A. Hawkridge n & D. Muddiman n

author keywords: N-linked glycans; HILIC; FTMS; Longitudinal sampling
topics (OpenAlex): Glycosylation and Glycoproteins Research; Cancer, Hypoxia, and Metabolism; Viral Infectious Diseases and Gene Expression in Insects
TL;DR: The initial interrogation of longitudinally-procured samples provides evidence that supports the value of this strategy in the search for glycomic biomarkers, and identifies 35 N-linked glycans identified in this study. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2

Muddiman, D., Andrews, G., Lewis, D., Notey, J., & Kelly, R. (2010, July 10). Analytical and Bioanalytical Chemistry, Vol. 398, pp. 377–389.

By: D. Muddiman n, G. Andrews n, D. Lewis n, J. Notey n & R. Kelly n

author keywords: Secretome; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Thermophile; Sample preparation
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Extracellular Space / metabolism; Gram-Positive Bacteria / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Enzyme Structure and Function; Protein Structure and Dynamics
TL;DR: In an effort to define the extracellular proteome of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, several techniques were considered regarding sample processing to achieve the most in-depth analysis of secreted proteins. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting

Muddiman, D., Andrews, G., Lewis, D., Notey, J., & Kelly, R. (2010, June 26). Analytical and Bioanalytical Chemistry, Vol. 398, pp. 391–404.

By: D. Muddiman n, G. Andrews n, D. Lewis n, J. Notey n & R. Kelly n

author keywords: Thermotoga maritima; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Co-cultivation; Intracellular proteome; Spectral counting
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chemotaxis; Chromatography, Liquid; Coculture Techniques; Electrophoresis, Polyacrylamide Gel; Fourier Analysis; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Intracellular Space / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis / statistics & numerical data; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Bacteriophages and microbial interactions; Genomics and Phylogenetic Studies
TL;DR: Altering in growth conditions of these bacterial thermophilic microorganisms offer a glimpse into the intricacy of microbial behavior and interaction as well as insight into microbial interaction and behavior. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Quantitative top-down proteomics of SILAC labeled human embryonic stem cells

Collier, T. S., Sarkar, P., Rao, B., & Muddiman, D. C. (2010, February 9). Journal of the American Society for Mass Spectrometry, Vol. 21, pp. 879–889.

By: T. Collier n, P. Sarkar n, B. Rao n & D. Muddiman n

Contributors: T. Collier n, P. Sarkar n, B. Rao n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Animals; Arginine / chemistry; Arginine / metabolism; Carbon Isotopes; Cell Line; Embryonic Stem Cells / chemistry; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Lysine / chemistry; Lysine / metabolism; Mass Spectrometry / methods; Mice; Proteome / analysis; Proteomics / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Amino Acid Enzymes and Metabolism
TL;DR: This study has confidently identified 11 proteins by accurate intact mass, MS/MS, and amino acid counting facilitated by SILAC labeling, which to the best of the authors' knowledge represents the first top-down analysis of hESCs. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Self-Assembly of Dendritic Tris(crown ether) Hexagons and Their Complexation with Dibenzylammonium Cations

Xu, X.-D., Yang, H.-B., Zheng, Y.-R., Ghosh, K., Lyndon, M. M., Muddiman, D. C., & Stang, P. J. (2010, September 29). The Journal of Organic Chemistry, Vol. 75, pp. 7373–7380.

MeSH headings : Benzylammonium Compounds / chemistry; Crown Ethers / chemistry; Dendrimers / chemistry; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Conformation
topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Molecular Sensors and Ion Detection; Supramolecular Chemistry and Complexes
TL;DR: Combining 120° crown ether-containing diplatinum(II) acceptors with 120° dendritic dipyridyl donors in a 1:1 ratio allows for the formation of a new family of dendrite triple crown ether derivatives with a hexagonal cavity in quantitative yields. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Study of the ionization mechanism in hybrid laser based desorption techniques

Dixon, R. B., & Muddiman, D. C. (2010, January 1). The Analyst, Vol. 135, pp. 880–882.

By: R. Dixon n & D. Muddiman n

topics (OpenAlex): Laser Design and Applications; Laser-Matter Interactions and Applications; Spectroscopy and Laser Applications
TL;DR: The purpose of these experiments is to test the hypothesis that the primary ionization pathway in MALDESI is through interaction of the laser-desorbed neutral species with the intersecting electrospray ionization beam. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

The effects of abundant plasma protein depletion on global glycan profiling using NanoLC FT-ICR mass spectrometry

Bereman, M. S., & Muddiman, D. C. (2010, January 19). Analytical and Bioanalytical Chemistry, Vol. 396, pp. 1473–1479.

By: M. Bereman n & D. Muddiman n

author keywords: HILIC; NanoLCFT-ICRMS; Glycan profiling; N-linked glycans; Protein depletion
MeSH headings : Blood Proteins / chemistry; Blood Proteins / standards; Chromatography, Liquid; Nanotechnology; Polysaccharides / chemistry; Polysaccharides / standards; Proteomics / instrumentation; Proteomics / methods; Reproducibility of Results; Spectroscopy, Fourier Transform Infrared
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: Protein depletion was found to marginally effect the abundance of high mannose, hybrid, and large highly sialylated complex species and small fucosylated non-sialylation complex glycans were found to decrease in abundance in the depleted samples. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Utilizing Spectral Counting To Quantitatively Characterize Tandem Removal of Abundant Proteins (TRAP) in Human Plasma

Shuford, C. M., Hawkridge, A. M., Burnett, J. C., & Muddiman, D. C. (2010, November 19). Analytical Chemistry, Vol. 82, pp. 10179–10185.

By: C. Shuford n, A. Hawkridge n, J. Burnett n & D. Muddiman n

MeSH headings : Biomarkers / analysis; Blood Proteins / analysis; Blood Proteins / isolation & purification; Humans
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Biosensors and Analytical Detection
TL;DR: The data demonstrate that this method, tandem removal of abundant proteins or TRAP, provides superior removal efficiency compared to traditional applications and improves the depth of proteome coverage in plasma. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Absolute Quantification of C-Reactive Protein in Human Plasma Derived from Patients with Epithelial Ovarian Cancer Utilizing Protein Cleavage Isotope Dilution Mass Spectrometry

Williams, D. K., & Muddiman, D. C. (2009, January 6). Journal of Proteome Research, Vol. 8, pp. 1085–1090.

By: D. Williams n & D. Muddiman n

author keywords: absolute quantification; C-reactive protein; ovarian cancer; single reaction monitoring
MeSH headings : Amino Acid Sequence; C-Reactive Protein / chemistry; C-Reactive Protein / metabolism; Chromatography, Liquid / methods; Female; Humans; Isotope Labeling / methods; Mass Spectrometry / methods; Molecular Sequence Data; Ovarian Neoplasms / blood; Ovarian Neoplasms / chemistry; Peptides / chemistry; Peptides / genetics; Peptides / metabolism; Reproducibility of Results
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: Interestingly, the PC-IDMS method provided concentration values that were approximately 10x the concentration reported by the ELISA method, which demonstrated that the method of detection is an important consideration when determining reference ranges of a particular analyte. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Atmospheric pressure infrared (10.6 µm) laser desorption electrospray ionization (IR‐LDESI) coupled to a LTQ Fourier transform ion cyclotron resonance mass spectrometer

Sampson, J. S., & Muddiman, D. C. (2009, June 5). Rapid Communications in Mass Spectrometry, Vol. 23, pp. 1989–1992.

By: J. Sampson n & D. Muddiman n

MeSH headings : Animals; Cattle; Molecular Weight; Proteins / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Electrohydrodynamics and Fluid Dynamics
TL;DR: The implementation of a CO(2) laser emitting infrared laser irradiation at 10.6 microm onto the versatile atmospheric pressure ionization platform is presented and Multiply charged proteins ranging in molecular mass from 8.6 to 17 kDa were detected from liquid-state samples without the addition of matrix. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Characterization of charge separation in the array of Micromachined UltraSonic Electrospray (AMUSE) ion source for mass spectrometry

Forbes, T. P., Dixon, R. B., Muddiman, D. C., Degertekin, F. L., & Fedorov, A. G. (2009, May 21). Journal of the American Society for Mass Spectrometry, Vol. 20, pp. 1684–1687.

MeSH headings : Equipment Design; Equipment Failure Analysis; Radiation Dosage; Radiometry; Sonication; Spectrometry, Mass, Electrospray Ionization / instrumentation; Static Electricity
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Electrohydrodynamics and Fluid Dynamics
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Coupling of a vented column with splitless nanoRPLC-ESI-MS for the improved separation and detection of brain natriuretic peptide-32 and its proteolytic peptides

Journal of Chromatography B, 877(10), 948–954.

By: G. Andrews n, C. Shuford n, J. Burnett*, A. Hawkridge n & D. Muddiman n

author keywords: Vented column; BNP-32; Splitless nanoLC; Tryptic digestion
MeSH headings : Amino Acid Sequence; Brain Chemistry; Chromatography, Liquid / methods; Molecular Sequence Data; Natriuretic Peptide, Brain / chemistry; Natriuretic Peptide, Brain / isolation & purification; Natriuretic Peptide, Brain / pharmacokinetics; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
TL;DR: It is demonstrated that the use of free cysteine as an alkylation quenching agent and a secondary digestion within the digestion scheme can provide targeted tryptic peptides with increased abundances, which will serve to further augment the detection of BNP-32 by LC-MS. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2009 article

Development of a Robust and High Throughput Method for Profiling N-Linked Glycans Derived from Plasma Glycoproteins by NanoLC−FTICR Mass Spectrometry

Bereman, M. S., Young, D. D., Deiters, A., & Muddiman, D. C. (2009, May 12). Journal of Proteome Research, Vol. 8, pp. 3764–3770.

By: M. Bereman n, D. Young n, A. Deiters n & D. Muddiman n

MeSH headings : Biomarkers, Tumor / metabolism; Blood Proteins / chemistry; Chromatography, Liquid / methods; Detergents / pharmacology; Glycoproteins / chemistry; Humans; Mass Spectrometry / methods; Microwaves; Peptides / chemistry; Polysaccharides / chemistry; Proteome; Proteomics / methods; Spectroscopy, Fourier Transform Infrared / methods; Time Factors
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Enzyme Structure and Function
TL;DR: A step-by-step protocol describing a method for N-linked glycan profiling of plasma glycoproteins by nanoflow liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Evaluation of the ALiPHAT method for PC-IDMS and correlation of limits-of-detection with nonpolar surface area

Williams, D. K., Comins, D. L., Whitten, J. L., & Muddiman, D. C. (2009, August 11). Journal of the American Society for Mass Spectrometry, Vol. 20, pp. 2006–2012.

By: D. Williams n, D. Comins n, J. Whitten n & D. Muddiman n

MeSH headings : Alkylation; Amino Acid Sequence; Calibration; Carbon Isotopes / chemistry; Chromatography, High Pressure Liquid / methods; Humans; Hydrophobic and Hydrophilic Interactions; Isotope Labeling / methods; Laminin / analysis; Laminin / chemistry; Male; Mass Spectrometry / methods; Nitrogen Isotopes / chemistry; Oligopeptides / analysis; Oligopeptides / chemistry; Prostate-Specific Antigen / chemistry; Reference Standards; Trypsin / analysis; Trypsin / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Identification and Quantification in Food
TL;DR: While an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophOBicity, the number of decreases in LOD was observed, the authors observed a decrease. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Improving limits of detection for B-type natriuretic peptide using PC-IDMS: An application of the ALiPHAT strategy

Shuford, C. M., Comins, D. L., Whitten, J. L., Burnett, J. C., Jr., & Muddiman, D. C. (2009, November 19). The Analyst, Vol. 135, pp. 36–41.

By: C. Shuford n, D. Comins n, J. Whitten n, J. Burnett* & D. Muddiman n

MeSH headings : Alkylating Agents / chemistry; Hydrophobic and Hydrophilic Interactions; Iodoacetamide / chemistry; Isotope Labeling; Limit of Detection; Natriuretic Peptide, Brain / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Trypsin / metabolism
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical chemistry methods development
TL;DR: Evaluated alkylation reagents for their utility in quantifying B-type Natriuretic Peptide, a low abundance cardiac biomarker, by protein cleavage isotope dilution mass spectrometry found a approximately 3.5-fold decrease in the detection limit for the best performing hydrophobic reagent, 2-iodo-N-octylacetamide. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Increasing the hydrophobicity and electrospray response of glycans through derivatization with novel cationic hydrazides

Bereman, M. S., Comins, D. L., & Muddiman, D. C. (2009, November 18). Chemical Communications, Vol. 46, pp. 237–239.

By: M. Bereman n, D. Comins n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Enzyme Catalysis and Immobilization; Electrohydrodynamics and Fluid Dynamics
TL;DR: Novel tags are used to increase the hydrophobicity of glycans and impart a permanent charge yielding as great as a approximately 5-fold increase in electrospray response from both a standard and complex mixture. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Mass Spectrometry–Based Biomarker Discovery: Toward a Global Proteome Index of Individuality

Hawkridge, A. M., & Muddiman, D. C. (2009, July 19). Annual Review of Analytical Chemistry, Vol. 2, pp. 265–277.

By: A. Hawkridge n & D. Muddiman n

author keywords: biological variability; analytical variability; longitudinal sampling
MeSH headings : Animals; Biomarkers / analysis; Humans; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Proteome / analysis; Proteomics / instrumentation; Proteomics / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The purpose of this review is to discuss the analytical methods and strategies that have been developed for mass spectrometry-based biomarker-discovery platforms and to place them in the context of the many challenges and opportunities yet to be addressed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Proteomics Characterization of Cell Membrane Blebs in Human Retinal Pigment Epithelium Cells

Alcazar, O., Hawkridge, A. M., Collier, T. S., Cousins, S. W., Bhattacharya, S. K., Muddiman, D. C., & Marin-Castano, M. E. (2009, June 30). Molecular & Cellular Proteomics, Vol. 8, pp. 2201–2211.

By: O. Alcazar*, A. Hawkridge n, T. Collier n, S. Cousins*, S. Bhattacharya*, D. Muddiman n, M. Marin-Castano*

MeSH headings : Aged; Animals; Antioxidants / pharmacology; Basigin / analysis; Cell Line; Cell Membrane / chemistry; Cell Membrane / drug effects; Cell Membrane / ultrastructure; Cell Surface Extensions / chemistry; Cell Surface Extensions / drug effects; Cell Surface Extensions / ultrastructure; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Epithelial Cells / chemistry; Epithelial Cells / cytology; Epithelial Cells / drug effects; Extracellular Matrix / chemistry; Extracellular Matrix / metabolism; Eye Proteins / analysis; Humans; Hydroquinones / pharmacology; Macular Degeneration / pathology; Matrix Metalloproteinase 14 / analysis; Matrix Metalloproteinase 2 / analysis; Molecular Sequence Data; Proteomics / methods; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Retinal Pigment Epithelium / chemistry; Retinal Pigment Epithelium / cytology; Smoke; Smoking / adverse effects; Tandem Mass Spectrometry; Tobacco / chemistry
topics (OpenAlex): Retinal Diseases and Treatments; Retinal Development and Disorders; Mosquito-borne diseases and control
TL;DR: Basigin and matrix metalloproteinase-14, key proteins involved in extracellular matrix remodeling, were identified in RPE blebs and shown to be more prevalent in AMD patients. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Species-dependent hepatic metabolism of immunosuppressive agent tacrolimus (FK-506)

Xenobiotica, 39(10), 757–765.

By: P. Tata*, N. Subbotina*, G. Burckart*, D. Muddiman*, A. Gusev, D. Hercules, T. Starzl*, R. Venkataramanan

author keywords: Tacrolimus; species differences; liver microsomes; cytochrome P-450; secondary ion mass spectrometry (SIMS); matrix-assisted laser desorption ionization (MALDI)
MeSH headings : Animals; Chromatography, High Pressure Liquid; Cricetinae; Cytochrome P-450 Enzyme System / metabolism; Dogs; Guinea Pigs; Humans; Immunosuppressive Agents / chemistry; Immunosuppressive Agents / metabolism; Kinetics; Microsomes, Liver / enzymology; Papio; Rabbits; Rats; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tacrolimus / chemistry; Tacrolimus / metabolism
topics (OpenAlex): Renal Transplantation Outcomes and Treatments; Pharmacological Effects and Toxicity Studies; HIV/AIDS drug development and treatment
TL;DR: From the current in-vitro metabolism studies, the pattern of tacroliums metabolism in baboons closely resembled that in humans and thus it is ideal for studying tacrolimus metabolism-related work of clinical relevance. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2009 article

Synthesis of Six-Component Metallodendrimers via [3 + 3] Coordination-Driven Self-Assembly

Yang, H.-B., Northrop, B. H., Zheng, Y.-R., Ghosh, K., Lyndon, M. M., Muddiman, D. C., & Stang, P. J. (2009, April 3). The Journal of Organic Chemistry, Vol. 74, pp. 3524–3527.

MeSH headings : Dendrimers / chemical synthesis; Dendrimers / chemistry; Models, Molecular; Molecular Conformation; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry
topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Supramolecular Chemistry and Complexes; Metal-Organic Frameworks: Synthesis and Applications
TL;DR: A new class of 120 degrees dendritic di-Pt(II) acceptor subunits has been designed and synthesized, from which six-component hexagonal metallodendrimers were easily formed with 120 degreesdendritic dipyridine donors via [3 + 3] coordination-driven self-assembly. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Tandem Mass Spectrometry of Thiolate-Protected Au Nanoparticles NaxAu25(SC2H4Ph)18−y(S(C2H4O)5CH3)y

Fields-Zinna, C. A., Sampson, J. S., Crowe, M. C., Tracy, J. B., Parker, J. F., deNey, A. M., … Murray, R. W. (2009, September 9). Journal of the American Chemical Society, Vol. 131, pp. 13844–13851.

By: C. Fields-Zinna n, J. Sampson n, M. Crowe n, J. Tracy n, J. Parker n, A. deNey n, D. Muddiman n, R. Murray n

Contributors: C. Fields Zinna n, J. Sampson n, M. Crowe n, J. Tracy n, J. Parker n, A. Deney n, D. Muddiman n, R. Murray n

MeSH headings : Crystallography, X-Ray; Gold / chemistry; Metal Nanoparticles / chemistry; Sulfhydryl Compounds / chemistry; Tandem Mass Spectrometry
topics (OpenAlex): Nanocluster Synthesis and Applications; Advanced Nanomaterials in Catalysis; Advanced biosensing and bioanalysis techniques
TL;DR: The first collision-induced dissociation tandem mass spectrometry (CID MS/MS) of a thiolate-protected Au nanoparticle that has a crystallographically determined structure is reported, and previously unreported results show that even soft ionization sources can result in Au nanoparticles fragmentation. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2009 article

Utilizing artificial neural networks in matlab to achieve parts-per-billion mass measurement accuracy with a fourier transform ion cyclotron resonance mass spectrometer

Williams, D. K., Kovach, A. L., Muddiman, D. C., & Hanck, K. W. (2009, March 12). Journal of the American Society for Mass Spectrometry, Vol. 20, pp. 1303–1310.

By: D. Williams n, A. Kovach n, D. Muddiman n & K. Hanck n

MeSH headings : Fourier Analysis; Linear Models; Mass Spectrometry / methods; Models, Theoretical; Neural Networks, Computer; Reproducibility of Results; Sensitivity and Specificity
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Chemical Sensor Technologies
TL;DR: Artificial neural network calibration in conjunction with automatic gain control (AGC) is utilized in these experiments to formally account for the differences in total ion population in the ICR cell between the external calibration spectra and experimental spectra. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

A New Family of Multiferrocene Complexes with Enhanced Control of Structure and Stoichiometry via Coordination-Driven Self-Assembly and Their Electrochemistry

Yang, H.-B., Ghosh, K., Zhao, Y., Northrop, B. H., Lyndon, M. M., Muddiman, D. C., … Stang, P. J. (2008, January 1). Journal of the American Chemical Society, Vol. 130, p. 839-.

MeSH headings : Electrochemistry; Ferrous Compounds / chemistry; Metallocenes; Molecular Structure; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry
topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Supramolecular Chemistry and Complexes; Molecular Junctions and Nanostructures
TL;DR: The design and synthesis of a new family of multiferrocene complexes with enhanced control of structure and stoichimetry via coordination-driven self-assembly is described, providing an enhanced understanding of the influence of structural factors on the electrochemistry of multifunctional electroactive supramolecular architectures. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Ambient Aerodynamic Ionization Source for Remote Analyte Sampling and Mass Spectrometric Analysis

Dixon, R. B., Sampson, J. S., Hawkridge, A. M., & Muddiman, D. C. (2008, June 5). Analytical Chemistry, Vol. 80, pp. 5266–5271.

By: R. Dixon n, J. Sampson n, A. Hawkridge n & D. Muddiman n

MeSH headings : Acetaminophen / antagonists & inhibitors; Acetaminophen / chemistry; Caffeine / analysis; Caffeine / chemistry; Natriuretic Peptide, Brain / analysis; Natriuretic Peptide, Brain / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ubiquitin / analysis; Ubiquitin / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: This novel aerodynamic device, herein termed remote analyte sampling, transport, and ionization relay (RASTIR) was used to remotely sample neutral species in the ambient and entrain them into an electrospray plume where they were subsequently ionized and detected using a linear ion trap Fourier transform mass spectrometer. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Calibration laws based on multiple linear regression applied to matrix‐assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry

Williams, D. K., Jr., Chadwick, M. A., Williams, T. I., & Muddiman, D. C. (2008, June 19). Journal of Mass Spectrometry, Vol. 43, pp. 1659–1663.

By: D. Williams n, M. Chadwick n, T. Williams n & D. Muddiman n

Contributors: D. Williams Jr. n, M. Chadwick n, T. Williams n & D. Muddiman n

author keywords: multiple linear regression; polypropylene glycol; calibration; matrix-assisted laser desorption/ionization (MALDI); Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS); space-charge effects
MeSH headings : Calibration; Cyclotrons; Fourier Analysis; Linear Models; Mass Spectrometry / methods; Multivariate Analysis; Peptide Mapping / methods; Peptide Mapping / statistics & numerical data; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: An evaluation of external mass calibration laws applied to MALDI-FT-ICR-MS is performed to achieve higher mass measurement accuracy (MMA) in Fourier transform ion cyclotron resonance mass spectrometry. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
1. No Poverty (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 article

Construction of a versatile high precision ambient ionization source for direct analysis and imaging

Sampson, J. S., Hawkridge, A. M., & Muddiman, D. C. (2008, June 29). Journal of the American Society for Mass Spectrometry, Vol. 19, pp. 1527–1534.

By: J. Sampson n, A. Hawkridge n & D. Muddiman n

MeSH headings : Bradykinin / chemistry; Cations / chemistry; Humans; Lasers; Melitten / chemistry; Muramidase / chemistry; Myoglobin / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ubiquitin / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The design and construction of a high precision ambient ionization source matrix-assisted laser desorption electrospray ionization (MALDESI) and the computer controlled high precision motion control system and high repetition rate Explorer laser are demonstrated during MALDESI-FT-ICR analysis. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2008 article

Coordination-Driven Face-Directed Self-Assembly of Trigonal Prisms. Face-Based Conformational Chirality

Caskey, D. C., Yamamoto, T., Addicott, C., Shoemaker, R. K., Vacek, J., Hawkridge, A. M., … Stang, P. J. (2008, May 21). Journal of the American Chemical Society, Vol. 130, pp. 7620–7628.

By: D. Caskey n, T. Yamamoto n, C. Addicott n, R. Shoemaker n, J. Vacek n, A. Hawkridge n, D. Muddiman n, G. Kottas n, J. Michl n, P. Stang n

MeSH headings : Magnetic Resonance Spectroscopy; Molecular Conformation; Pyridines / chemistry; Spectrometry, Mass, Electrospray Ionization; Stereoisomerism; Temperature
topics (OpenAlex): Supramolecular Self-Assembly in Materials; Supramolecular Chemistry and Complexes; Molecular Sensors and Ion Detection
TL;DR: NMR data prove that the two edges of the pyridine rings in the triflate salts of 3a-3d are distinct and molecular mechanics results suggest that in the smallest prism 3d collective effects dominate and the all-P and all-M conformers are strongly favored. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Coordination-Driven Self-Assembly of Cavity-Cored Multiple Crown Ether Derivatives and Poly[2]pseudorotaxanes

Ghosh, K., Yang, H.-B., Northrop, B. H., Lyndon, M. M., Zheng, Y.-R., Muddiman, D. C., & Stang, P. J. (2008, March 15). Journal of the American Chemical Society, Vol. 130, pp. 5320–5334.

MeSH headings : Crown Ethers / chemical synthesis; Crown Ethers / chemistry; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Polymers / chemistry; Rotaxanes / chemistry; Spectrometry, Mass, Electrospray Ionization; Thermodynamics
topics (OpenAlex): Supramolecular Chemistry and Complexes; Supramolecular Self-Assembly in Materials; Porphyrin and Phthalocyanine Chemistry
TL;DR: The synthesis of a new 120 degree diplatinum(II) acceptor unit and the self-assembly of a series of two-dimensional metallacyclic polypseudorotaxanes that utilize both metal-ligand and crown ether-dialkylammonium noncovalent interactions are described, demonstrating the utility and scope of this orthogonal self- assembly technique. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Development and Characterization of an Ionization Technique for Analysis of Biological Macromolecules: Liquid Matrix-Assisted Laser Desorption Electrospray Ionization

Sampson, J. S., Hawkridge, A. M., & Muddiman, D. C. (2008, July 26). Analytical Chemistry, Vol. 80, pp. 6773–6778.

By: J. Sampson n, A. Hawkridge n & D. Muddiman n

MeSH headings : Organic Chemicals / chemistry; Peptides / analysis; Proteins / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Surface Properties
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical Chemistry and Chromatography
TL;DR: An increase in ion abundance and a concurrent decrease in surface tension with an increase in the applied potential are demonstrated and a singly charged radical cation of an electrochemically active species indicating oxidation occurs for analytes and therefore water is observed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Development of a nanoLC LTQ Orbitrap Mass Spectrometric Method for Profiling Glycans Derived from Plasma from Healthy, Benign Tumor Control, and Epithelial Ovarian Cancer Patients

Bereman, M. S., Williams, T. I., & Muddiman, D. C. (2008, December 29). Analytical Chemistry, Vol. 2, pp. 1130–1136.

By: M. Bereman n, T. Williams n & D. Muddiman n

Contributors: M. Bereman n, T. Williams n & D. Muddiman n

MeSH headings : Biomarkers, Tumor / blood; Biomarkers, Tumor / chemistry; Carcinoma / diagnosis; Chromatography, Liquid / methods; Female; Genital Neoplasms, Female / chemistry; Genital Neoplasms, Female / diagnosis; Glycoproteins / blood; Glycoproteins / chemistry; Humans; Hydrophobic and Hydrophilic Interactions; Lectins / blood; Lectins / chemistry; Male; Ovarian Neoplasms / diagnosis; Polysaccharides / blood; Polysaccharides / chemistry; ROC Curve; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Analytical Chemistry and Chromatography; Mass Spectrometry Techniques and Applications
TL;DR: A split-less nano-flow liquid chromatography mass spectrometric analysis of glycans chemically cleaved from glycoproteins in plasma shows similar column efficiencies and excellent retention time reproducibility without an internal standard to correct for retention time shift. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 journal article

Effect of Plasma Protein Depletion on BNP-32 Recovery

Clinical Chemistry, 54(5), 933–934.

By: A. Hawkridge n, D. Muddiman n, D. Hebulein*, A. Cataliotti* & J. Burnett*

MeSH headings : Blood Proteins / metabolism; Humans; Iodine Radioisotopes; Natriuretic Peptide, Brain / blood; Protein Binding
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Advanced biosensing and bioanalysis techniques
TL;DR: A targeted biomarker discovery platform for characterizing the circulating forms of B-type natriuretic peptide that includes protein depletion strategies, immunoprecipitation, gel electrophoresis, isotope dilution (absolute quantification), and nanoflow liquid chromatography coupled to high-performance hybrid Fourier transform mass spectrometry is developed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2008 article

Generation of multiply charged peptides and proteins by radio frequency acoustic desorption and ionization for mass spectrometric detection

Dixon, R. B., Sampson, J. S., & Muddiman, D. C. (2008, December 7). Journal of the American Society for Mass Spectrometry, Vol. 20, pp. 597–600.

By: R. Dixon n, J. Sampson n & D. Muddiman n

MeSH headings : Electrodes; Equipment Design; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Natriuretic Peptide, Brain / chemistry; Peptides / chemistry; Proteins / chemistry; Quartz / chemistry; Ubiquitin / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Chemical Sensor Technologies; Analytical Chemistry and Chromatography
TL;DR: The design and implementation of a radio frequency acoustic desorption ionization (RADIO) source has been demonstrated and intact and dissociated species observed during MS and MS/MS experiments, respectively are confirmed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Human Proteinpedia enables sharing of human protein data

Nature Biotechnology, 26(2), 164–167.

By: D. Muddiman (1 connected NC State author and many others, 160 authors in total )

MeSH headings : Database Management Systems; Databases, Protein; Gene Expression Profiling / methods; Information Storage and Retrieval / methods; Internationality; Internet; Magnetic Resonance Spectroscopy / methods; Peptide Mapping / methods; Proteome / chemistry; Proteome / classification; Proteome / metabolism; Proteomics / methods; Software; User-Computer Interface
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Bioinformatics and Genomic Networks; Gene expression and cancer classification
TL;DR: MMCD is a practical tool for expediting the time-consuming steps of identifying and researching small molecules and is compatible with both NMR and MS data and facilitates high-throughput metabolomics investigations. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2008 personal communication

Human Proteinpedia enables sharing of human protein data

By: S. Mathivanan, M. Ahmed, N. Ahn, H. Alexandre, R. Amanchy, P. Andrews, J. Bader, B. Balgley ...

Source: NC State University Libraries
Added: August 6, 2018

2008 article

Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry

Sampson, J. S., Murray, K. K., & Muddiman, D. C. (2008, December 13). Journal of the American Society for Mass Spectrometry, Vol. 20, pp. 667–673.

By: J. Sampson n, K. Murray* & D. Muddiman n

MeSH headings : Amino Acid Sequence; Animals; Carbohydrates / chemistry; Cattle; Chickens; Egg Yolk / chemistry; Horses; Lipids / chemistry; Milk / chemistry; Molecular Sequence Data; Mucins / chemistry; Mucins / metabolism; Myoglobin / chemistry; Polysaccharides / chemistry; Polysaccharides / metabolism; Proteins / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
TL;DR: Three of the four classes of biological macromolecules have been ionized and detected using MALDESI with minimal sample preparation and Sequencing of O-linked glycans, cleaved from mucin using reductive β-elimination chemistry, is also demonstrated. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Investigations with O‐linked protein glycosylations by matrix‐assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry

Williams, T. I., Saggese, D. A., Toups, K. L., Frahm, J. L., An, H. J., Li, B., … Muddiman, D. C. (2008, March 6). Journal of Mass Spectrometry, Vol. 43, pp. 1215–1223.

By: T. Williams n, D. Saggese n, K. Toups n, J. Frahm n, H. An*, B. Li*, C. Lebrilla*, D. Muddiman n

Contributors: T. Williams n, D. Saggese n, K. Toups n, J. Frahm n, H. An*, B. Li*, C. Lebrilla*, D. Muddiman n

author keywords: Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS); matrix-assisted laser desorption/ionization (MALDI); mucin; O-linked glycans; protein glycosylations
MeSH headings : Angiotensins / chemistry; Animals; Glycosylation; Mucins / chemistry; Oligosaccharides / chemistry; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Swine; Tandem Mass Spectrometry / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; RNA and protein synthesis mechanisms
TL;DR: A detailed examination of sample preparation and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT-ICR-MS) analysis of O-linked glycans globally cleaved from mucin glycoproteins is presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 article

Mass measurement accuracy comparisons between a double‐focusing magnetic sector and a time‐of‐flight mass analyzer

Bereman, M. S., Lyndon, M. M., Dixon, R. B., & Muddiman, D. C. (2008, April 17). Rapid Communications in Mass Spectrometry.

By: M. Bereman n, M. Lyndon n, R. Dixon n & D. Muddiman n

MeSH headings : Equipment Design; Equipment Failure Analysis; Magnetics / instrumentation; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
TL;DR: A direct comparison of the mass measurement accuracies (MMAs) obtained on different mass spectrometry instrument types; a magnetic sector as the 'gold standard' and an electrospray ionization time-of-flight (ESI-TOF) instrument. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Studying O-Linked Protein Glycosylations in Human Plasma

Williams, T. I., Saggese, D. A., & Muddiman, D. C. (2008, April 19). Journal of Proteome Research.

By: T. Williams n, D. Saggese n & D. Muddiman n

Contributors: T. Williams n, D. Saggese n & D. Muddiman n

author keywords: protein O-glycosylations; plasma; epithelial ovarian cancer (EOC); matrix-assisted laser desorption/iozation (MALDI); Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR-MS)
MeSH headings : Blood Proteins / chemistry; Blood Proteins / metabolism; Borohydrides / chemistry; Dialysis / methods; Female; Glycosylation; Hexosamines / analysis; Hexoses / analysis; Hexuronic Acids / analysis; Humans; Middle Aged; Ovarian Neoplasms / blood; Polysaccharides / analysis; Polysaccharides / chemistry; Polysaccharides / isolation & purification; Pronase / metabolism; Solid Phase Extraction / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Galectins and Cancer Biology; Carbohydrate Chemistry and Synthesis
TL;DR: The protocol here identifies O-linked carbohydrate patterns in EOC plasma glycoproteins through chemical cleavage and purification of these glycans through chemical Cleavage and Purification ofThese glycans are identified through carbohydrate pattern analysis with MALDI-FT-ICR-MS. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 journal article

Synthesis, Characterization, and Application of Iodoacetamide Derivatives Utilized for the ALiPHAT Strategy

Journal of the American Chemical Society, 130(7), 2122–2123.

By: D. Williams n, C. Meadows n, I. Bori n, A. Hawkridge n, D. Comins n & D. Muddiman n

MeSH headings : Alkylation; Amino Acid Sequence; Cysteine / chemistry; Factor VIIIa / antagonists & inhibitors; Hydrophobic and Hydrophilic Interactions; Iodoacetamide / analogs & derivatives; Iodoacetamide / chemical synthesis; Iodoacetamide / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Chemical Synthesis and Analysis; Mass Spectrometry Techniques and Applications; Peptidase Inhibition and Analysis
TL;DR: Hydrophobic tags, derived from iodoacetamide, were synthesized, characterized, and utilized to alkylate peptide E-76, a known inhibitor of coagulation factor VIIIa, and two other cysteine containing peptides to demonstrate an increase in electrospray response. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2008 article

Synthesis, characterization, and application of lodoacetamide derivatives utilized for the ALiPHAT strategy

Journal of the American Chemical Society, Vol. 130, p. 2122-.

By: D. Williams, C. Meadows, I. Bori, A. Hawkridge, D. Comins & D. Muddiman

topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2008 article

Temperature-Dependent Regulation of Proteins in Aspergillus flavus: Whole Organism Stable Isotope Labeling by Amino Acids

Georgianna, D. R., Hawkridge, A. M., Muddiman, D. C., & Payne, G. A. (2008, June 5). Journal of Proteome Research, Vol. 7, pp. 2973–2979.

By: D. Georgianna n, A. Hawkridge n, D. Muddiman n & G. Payne n

author keywords: SILAC; mass spectrometry; proteomics; Aspergillus flavus; aflatoxin
MeSH headings : Aflatoxins / biosynthesis; Amino Acids / metabolism; Arginine / metabolism; Aspergillus flavus / metabolism; Carbon Isotopes; Chromatography, Liquid; Culture Media; Fungal Proteins / biosynthesis; Fungal Proteins / genetics; Gene Expression Regulation, Fungal; Isotope Labeling; Oligonucleotide Array Sequence Analysis; Tandem Mass Spectrometry; Temperature
topics (OpenAlex): Mycotoxins in Agriculture and Food; Advanced Proteomics Techniques and Applications; Fungal and yeast genetics research
TL;DR: The utility of this procedure in quantifying changes in protein expression in response to environmental stimuli is shown, and the first reported labeling of a multicellular free-living prototroph using the SILAC procedure to compare (13)C(6)-arginine-labeled samples to (12)C (6-arginines-labeling samples for quantitative proteomics is reported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Top-Down Identification and Quantification of Stable Isotope Labeled Proteins from Aspergillus flavus Using Online Nano-Flow Reversed-Phase Liquid Chromatography Coupled to a LTQ-FTICR Mass Spectrometer

Collier, T. S., Hawkridge, A. M., Georgianna, D. R., Payne, G. A., & Muddiman, D. C. (2008, May 31). Analytical Chemistry, Vol. 80, pp. 4994–5001.

By: T. Collier n, A. Hawkridge n, D. Georgianna n, G. Payne n & D. Muddiman n

MeSH headings : Arginine / chemistry; Arginine / metabolism; Aspergillus flavus / chemistry; Aspergillus flavus / metabolism; Carbon Radioisotopes / chemistry; Chromatography, Liquid / methods; Fourier Analysis; Fungal Proteins / analysis; Fungal Proteins / chemistry; Fungal Proteins / metabolism; Isotope Labeling; Tandem Mass Spectrometry / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: The qualitative and quantitative top-down characterization of proteins derived from SILAC-labeled Aspergillus flavus is described using nanoflow reversed-phase liquid chromatography directly coupled to a linear ion trap Fourier transform ion cyclotron resonance mass spectrometer (nLC-LTQ-FTICR-MS). (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

A Highly Efficient Approach to the Self-Assembly of Hexagonal Cavity-Cored Tris[2]pseudorotaxanes from Several Components via Multiple Noncovalent Interactions

Yang, H.-B., Ghosh, K., Northrop, B. H., Zheng, Y.-R., Lyndon, M. M., Muddiman, D. C., & Stang, P. J. (2007, October 27). Journal of the American Chemical Society, Vol. 129, p. 14187.

By: H. Yang n, K. Ghosh n, B. Northrop n, Y. Zheng n, M. Lyndon n, D. Muddiman n, P. Stang n

MeSH headings : Crystallography, X-Ray; Magnetic Resonance Spectroscopy; Models, Chemical; Organoplatinum Compounds / chemistry; Rotaxanes / chemical synthesis; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Supramolecular Chemistry and Complexes; Metal-Organic Frameworks: Synthesis and Applications; Supramolecular Self-Assembly in Materials
TL;DR: This methodology demonstrates the power of orthogonal self-assembly and may be expanded beyond the use of two recognition motifs in hopes of mimicking the complex multifunctionality of biological systems. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Achieving Augmented Limits of Detection for Peptides with Hydrophobic Alkyl Tags

Frahm, J. L., Bori, I. D., Comins, D. L., Hawkridge, A. M., & Muddiman, D. C. (2007, May 4). Analytical Chemistry, Vol. 79, pp. 3989–3995.

By: J. Frahm n, I. Bori n, D. Comins n, A. Hawkridge n & D. Muddiman n

MeSH headings : Acetates / chemistry; Alkylation; Amides / chemical synthesis; Amides / chemistry; Amino Acid Sequence; Chlorides / chemistry; Hydrophobic and Hydrophilic Interactions; Iodine / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Advanced biosensing and bioanalysis techniques
TL;DR: The results demonstrate the feasibility of the octylcarboxyamidomethyl modification to improve limits of detection for cysteine-containing peptides with hydrophobic alkyl tags. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Carbohydrate Analysis by Desorption Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Bereman, M. S., Williams, T. I., & Muddiman, D. C. (2007, October 6). Analytical Chemistry, Vol. 79, pp. 8812–8815.

By: M. Bereman n, T. Williams n & D. Muddiman n

Contributors: M. Bereman n, T. Williams n & D. Muddiman n

MeSH headings : Carbohydrates / analysis; Cyclotrons; Fourier Analysis; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: G glycans from mucin are identified by using the high mass measurement accuracy and tandem MS capabilities afforded by the hybrid FT-ICR-MS platform. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 article

Coordination-Driven Self-Assembly of Metallodendrimers Possessing Well-Defined and Controllable Cavities as Cores

Yang, H.-B., Hawkridge, A. M., Huang, S. D., Das, N., Bunge, S. D., Muddiman, D. C., & Stang, P. J. (2007, January 27). Journal of the American Chemical Society, Vol. 129, pp. 2120–2129.

MeSH headings : Alkynes / chemical synthesis; Alkynes / chemistry; Crystallography, X-Ray; Dendrimers / chemical synthesis; Dendrimers / chemistry; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry; Organoplatinum Compounds / chemical synthesis; Organoplatinum Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Supramolecular Self-Assembly in Materials; Supramolecular Chemistry and Complexes; Crystallography and molecular interactions
TL;DR: Isotopically resolved mass spectrometry data support the existence of the metallodendrimers with rhomboidal and hexagonal cavities, and NMR data are consistent with the formation of all ensembles. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Details of the Structure Determination of the Sulfated Steroids PSDS and PADS:  New Components of the Sea Lamprey (Petromyzonmarinus) Migratory Pheromone

The Journal of Organic Chemistry, 72(20), 7544–7550.

By: T. Hoye n, V. Dvornikovs n, J. Fine n, K. Anderson n, C. Jeffrey n, D. Muddiman n, F. Shao n, P. Sorensen n, J. Wang n

MeSH headings : Animals; Cholestanes / chemistry; Molecular Conformation; Nuclear Magnetic Resonance, Biomolecular; Petromyzon / metabolism; Pheromones / chemistry; Pyrrolidinones / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Identification and Quantification in Food; Reproductive biology and impacts on aquatic species
TL;DR: Details of the structure determination of these steroidal bis-sulfates of the migratory pheromone used by sea lamprey to guide adults to spawning grounds are described and logic underlying the iterative analyses used is presented. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
14. Life Below Water (Web of Science; OpenAlex)
15. Life on Land (Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2007 article

Details of the structure determination of the sulfated steroids PSDS and PADS: New components of the sea lamprey (Petromyzon marinus) migratory pheromone

Journal of Organic Chemistry, Vol. 72, pp. 7544–7550.

By: T. Hoye, V. Dvornikovs, J. Fine, K. Anderson, C. Jeffrey, D. Muddiman, F. Shao, P. Sorensen, J. Wang

topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2007 article

Detection of Attomole Amounts of Analyte by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) Determined Using Fluorescence Spectroscopy

Bereman, M. S., & Muddiman, D. C. (2007, March 22). Journal of the American Society for Mass Spectrometry, Vol. 18, pp. 1093–1096.

By: M. Bereman n & D. Muddiman n

MeSH headings : Microchemistry / methods; Nanotechnology / methods; Rhodamines / chemistry; Spectrometry, Fluorescence / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Analytical chemistry methods development
TL;DR: The use of fluorescence spectroscopy is reported to investigate the amount of material removed from a PTFE surface and detected during desorption electrospray ionization (DESI) mass spectrometry measurements. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Differential protein expression in male and female human lumbar cerebrospinal fluid using iTRAQ reagents after abundant protein depletion

Ogata, Y., Charlesworth, M. C., Higgins, L. A., Keegan, B. M., Vernino, S., & Muddiman, D. C. (2007, September 13). PROTEOMICS, Vol. 7, pp. 3726–3734.

By: Y. Ogata*, M. Charlesworth*, L. Higgins*, B. Keegan*, S. Vernino* & D. Muddiman n

author keywords: ceruloplasmin; ferroxidase; gender; low-abundance protein; mass spectrometry
MeSH headings : Adult; Aged; Biomarkers / analysis; Biomarkers / metabolism; Cerebrospinal Fluid Proteins / analysis; Cerebrospinal Fluid Proteins / metabolism; Female; Humans; Indicators and Reagents; Lumbosacral Region; Male; Middle Aged; Proteome / analysis; Proteome / metabolism
topics (OpenAlex): S100 Proteins and Annexins; Advanced Proteomics Techniques and Applications; Enzyme Structure and Function
TL;DR: This study examined the differential expression of proteins in pooled male and female CSF utilizing isobaric tags for relative and absolute quantification (iTRAQ™) reagents after the depletion of six high abundant proteins using a multiple affinity removal system (MARS). (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Direct characterization of intact polypeptides by matrix‐assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry

Sampson, J. S., Hawkridge, A. M., & Muddiman, D. C. (2007, February 23). Rapid Communications in Mass Spectrometry, Vol. 21, pp. 1150–1154.

By: J. Sampson n, A. Hawkridge n & D. Muddiman n

MeSH headings : Cyclotrons; Microchemistry / methods; Molecular Weight; Peptides / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: The ability of MALDESI-QFT-ICR MS to directly analyze and provide high mass measurement accuracy (approximately 1 part-per-million) of a polypeptide using internal calibration is demonstrated and the potential of this system for the top-down characterization of multiply charged polyPEptide cations is shown. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Dual Electrospray Ion Source for Electron-Transfer Dissociation on a Hybrid Linear Ion Trap−Orbitrap Mass Spectrometer

D. Keith Williams, McAlister, G. C., Good, D. M., Coon, J. J., & Muddiman, D. C. (2007, September 15). Analytical Chemistry, Vol. 79, pp. 7916–7919.

By: . D. Keith Williams n, G. McAlister n, D. Good n, J. Coon n & D. Muddiman n

MeSH headings : Electrons; Ions; Molecular Structure; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
TL;DR: The stability of the ETD anion population is demonstrated and the ability to identify several c- and z-type product ions from multiply protonated peptide cations is demonstrated. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Effect of matrix crystal structure on ion abundance of carbohydrates by matrix‐assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry

Williams, T. I., Saggese, D. A., Wilcox, R. J., Martin, J. D., & Muddiman, D. C. (2007, February 5). Rapid Communications in Mass Spectrometry, Vol. 21, pp. 807–811.

By: T. Williams n, D. Saggese n, R. Wilcox n, J. Martin n & D. Muddiman n

Contributors: T. Williams n, D. Saggese n, R. Wilcox n, J. Martin n & D. Muddiman n

MeSH headings : Acetylglucosamine / chemistry; Crystallization; Cyclotrons; Ions; N-Acetylneuraminic Acid / chemistry; Powder Diffraction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: Sample preparation techniques for carbohydrate analysis using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are explored, with particular emphasis on analyte/matrix co-crystallization procedures. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

Elevated Levels of Phosphorylated Fibrinogen-α-Iso-forms and Differential Expression of Other Post-Translationally Modified Proteins in the Plasma of Ovarian Cancer PatientsJ. Proteome Res.2006,5, 3318−3325.

Journal of Proteome Research, 6(4), 1615–1615.

By: Y. Ogata, C. Heppelmann, M. Charlesworth, B. Madden, M. Miller, K. Kalli, W. Cilby, H. Bergen, D. Saggese, D. Muddiman*

topics (OpenAlex): Cancer, Lipids, and Metabolism; Advanced Proteomics Techniques and Applications
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2007 article

Epithelial Ovarian Cancer:  Disease Etiology, Treatment, Detection, and Investigational Gene, Metabolite, and Protein Biomarkers

Williams, T. I., Toups, K. L., Saggese, D. A., Kalli, K. R., Cliby, W. A., & Muddiman, D. C. (2007, June 21). Journal of Proteome Research, Vol. 8, pp. 2936–2962.

By: T. Williams n, K. Toups n, D. Saggese n, K. Kalli n, W. Cliby n & D. Muddiman n

Contributors: T. Williams n, K. Toups n, D. Saggese n, K. Kalli n, W. Cliby n & D. Muddiman n

author keywords: biomarkers; epithelial ovarian cancer; proteomics; mass spectrometry; detection; treatments
MeSH headings : Biomarkers, Tumor / metabolism; Female; Humans; Neoplasms, Glandular and Epithelial / diagnosis; Neoplasms, Glandular and Epithelial / etiology; Neoplasms, Glandular and Epithelial / metabolism; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / etiology; Ovarian Neoplasms / metabolism
topics (OpenAlex): Ovarian cancer diagnosis and treatment; Cancer, Lipids, and Metabolism; RNA modifications and cancer
TL;DR: An introduction to ovarian cancer is offered, with particular emphasis on human epithelial ovarian cancer, and a survey of promising new protein, gene, and metabolite biomarkers on the horizon is provided. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 article

Parts-Per-Billion Mass Measurement Accuracy Achieved through the Combination of Multiple Linear Regression and Automatic Gain Control in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Williams, D. K., & Muddiman, D. C. (2007, June 1). Analytical Chemistry, Vol. 79, pp. 5058–5063.

By: D. Williams n & D. Muddiman n

MeSH headings : Automation; Calibration; Cyclotrons / instrumentation; Ions; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: Calibrated multiple linear regression calibration is used to account for both differences in total ion population in the ICR cell as well as relative ion abundance of a given species, which affords mean MMA values at the parts-per-billion (ppb) level. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Probing the mechanisms of an air amplifier using a LTQ-FT-ICR-MS and fluorescence spectroscopy

Dixon, R. B., Muddiman, D. C., Hawkridge, A. M., & Fedorov, A. G. (2007, August 17). Journal of the American Society for Mass Spectrometry, Vol. 18, pp. 1909–1913.

By: R. Dixon n, D. Muddiman n, A. Hawkridge n & A. Fedorov*

MeSH headings : Air; Amplifiers, Electronic; Cyclotrons; Fluorescent Dyes / chemistry; Spectrometry, Fluorescence / instrumentation; Spectrometry, Fluorescence / methods; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical chemistry methods development; Microfluidic and Capillary Electrophoresis Applications
TL;DR: Experimental results provide direct evidence of aerodynamic focusing of electrosprayed droplets/ions enabled by an air amplifier, resulting in improved Electrospray droplet/ion capture efficiency and reduced ion injection time. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Quantitative comparison of a flared and a standard heated metal capillary inlet with a voltage‐assisted air amplifier on an electrospray ionization linear ion trap mass spectrometer

Dixon, R. B., & Muddiman, D. C. (2007, September 3). Rapid Communications in Mass Spectrometry, Vol. 21, pp. 3207–3212.

By: R. Dixon n & D. Muddiman n

MeSH headings : Air; Amplifiers, Electronic; Capillary Action; Electromagnetic Phenomena / instrumentation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis / instrumentation; Flow Injection Analysis / methods; Formates / analysis; Heating / instrumentation; Melitten / analysis; Microfluidics / instrumentation; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / instrumentation
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Electrohydrodynamics and Fluid Dynamics; Microfluidic and Capillary Electrophoresis Applications
TL;DR: The performance characteristics of a flared and blunt-ended heated metal capillary were evaluated with a voltage-assisted air amplifier on a linear ion trap mass spectrometer and demonstrated that a standard capillary afforded higher ion abundance than a flared capillary. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

Remote mass spectrometric sampling of electrospray- and desorption electrospra-generated ions using an air ejector

Journal of the American Society for Mass Spectrometry, Vol. 18, pp. 1844–1847.

By: R. Dixon, M. Bereman, D. Muddiman & A. Hawkridge

topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2007 journal article

Remote mass spectrometric sampling of electrospray- and desorption electrospray-generated ions using an air ejector

Journal of the American Society for Mass Spectrometry, 18(10), 1844–1847.

By: R. Dixon n, M. Bereman n, D. Muddiman n & A. Hawkridge n

MeSH headings : Air; Environmental Monitoring / instrumentation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis / instrumentation; Flow Injection Analysis / methods; Rheology / instrumentation; Rheology / methods; Robotics / instrumentation; Specimen Handling / instrumentation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: This work demonstrates the remote analysis of a series of analyte ions that range from small molecules and polymers to polypeptides using the AE-LTQ interface and presents preliminary mass spectrometric data. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2007 article

Understanding the influence of post‐excite radius and axial confinement on quantitative proteomic measurements using Fourier transform ion cyclotron resonance mass spectrometry

Frahm, J. L., Velez, C. M. C., & Muddiman, D. C. (2007, February 28). Rapid Communications in Mass Spectrometry, Vol. 21, pp. 1196–1204.

By: J. Frahm n, C. Velez* & D. Muddiman n

MeSH headings : Artifacts; Cyclotrons; Ions; Peptide Mapping / methods; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Sequence Analysis, Protein / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: To adequately assess the multiple parameters in the large dataset, a multiplicative quality factor is developed that encompasses the total ion abundance, as well as the accuracy and the precision of abundance ratios, which allows facile determination of optimal instrument parameters for quantitative measurements. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 article

"Lewis & Clark" Proteomics

Muddiman, D. C. (2006, February 1). Journal of Proteome Research, Vol. 5, pp. 221–222.

By: D. Muddiman*

MeSH headings : Animals; Biomarkers / chemistry; Blood Proteins / chemistry; Cells, Cultured; Disease Models, Animal; Famous Persons; History, 19th Century; Humans; Proteomics
topics (OpenAlex): Advanced Proteomics Techniques and Applications
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 article

A Method for Automatically Interpreting Mass Spectra of 18O-Labeled Isotopic Clusters

Mason, C. J., Therneau, T. M., Eckel-Passow, J. E., Johnson, K. L., Oberg, A. L., Olson, J. E., … Bergen, H. R. (2006, October 26). Molecular & Cellular Proteomics, Vol. 6, pp. 305–318.

By: C. Mason*, T. Therneau*, J. Eckel-Passow*, K. Johnson*, A. Oberg*, J. Olson*, K. Nair*, D. Muddiman n, H. Bergen*

MeSH headings : Algorithms; Chromatography, High Pressure Liquid; Cluster Analysis; Computational Biology; Mass Spectrometry / methods; Oxygen Isotopes / metabolism; Peptide Mapping; Proteome / metabolism; Proteomics / methods; Regression Analysis
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Cancer, Hypoxia, and Metabolism
TL;DR: An algorithm entitled regression analysis applied to mass spectrometry (RAAMS) is developed that automatically, rapidly, and confidently interprets spectra of 18O-labeled peptides without requiring chemical composition information derived from product ion spectra. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 article

Accessible proteomics space and its implications for peak capacity for zero‐, one‐ and two‐dimensional separations coupled with FT‐ICR and TOF mass spectrometry

Frahm, J. L., Howard, B. E., Heber, S., & Muddiman, D. C. (2006, March 1). Journal of Mass Spectrometry, Vol. 41, pp. 281–288.

By: J. Frahm n, B. Howard n, S. Heber n & D. Muddiman n

MeSH headings : Biotechnology / instrumentation; Biotechnology / methods; Electrophoresis, Gel, Two-Dimensional; Fourier Analysis; Mass Spectrometry; Peptides / analysis; Proteomics / instrumentation; Proteomics / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
TL;DR: The results indicate that reverse-phase-nanoHPLC (RP-nHPLC) separation coupled with FT-ICR MS offers an order of magnitude improvement in peak capacity over RP- nHPLC separation coupling with TOF MS, and the addition of an orthogonal separation method, strong cation exchange (SCX), for 2D LC-MS demonstrates an additional 10-fold improvement inpeak capacity over 1D LC -MS methods. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Chromatin Assembly Factor 1 Interacts with Histone H3 Methylated at Lysine 79 in the Processes of Epigenetic Silencing and DNA Repair†

Biochemistry, 45(9), 2852–2861.

By: H. Zhou*, B. Madden*, D. Muddiman* & Z. Zhang*

MeSH headings : Amino Acid Sequence; Cell Extracts; Chromatin Assembly Factor-1; Chromosomal Proteins, Non-Histone / genetics; Chromosomal Proteins, Non-Histone / isolation & purification; Chromosomal Proteins, Non-Histone / metabolism; DNA Repair / physiology; DNA-Binding Proteins / genetics; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Epigenesis, Genetic / physiology; Gene Silencing; Histones / metabolism; Lysine / metabolism; Mass Spectrometry; Methylation; Molecular Sequence Data; Nucleosomes / metabolism; S Phase; Saccharomyces cerevisiae / metabolism; Silent Information Regulator Proteins, Saccharomyces cerevisiae / metabolism
topics (OpenAlex): Epigenetics and DNA Methylation; Genomics and Chromatin Dynamics; DNA Repair Mechanisms
TL;DR: Together, these data indicate that CAF-1 interacts with H3 methylated at lysine 79 during the processes of epigenetic silencing and DNA repair. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2006 journal article

Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 20(22), 3409–3411.

By: M. Bereman n, L. Nyadong*, F. Fernandez* & D. Muddiman n

MeSH headings : Bradykinin / analysis; Formates / analysis; Glucagon / analysis; Insulin / analysis; Melitten / analysis; Peptides / analysis; Proteins / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared; Vasodilator Agents / analysis
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: The first coupling of a desorption electrospray ionization (DESI) ion source to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) for high-resolution protein analysis is reported. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2006 article

Elevated Levels of Phosphorylated Fibrinogen-α-Isoforms and Differential Expression of Other Post-Translationally Modified Proteins in the Plasma of Ovarian Cancer Patients

Ogata, Y., Heppelmann, C. J., Charlesworth, M. C., Madden, B. J., Miller, M. N., Kalli, K. R., … Muddiman, D. C. (2006, November 14). Journal of Proteome Research, Vol. 5, pp. 3318–3325.

By: Y. Ogata n, C. Heppelmann n, M. Charlesworth n, B. Madden n, M. Miller n, K. Kalli n, W. Cliby n, H. Bergen n, D. Saggese n, D. Muddiman n

author keywords: ovarian cancer; biomarker; fibrinogen; two-dimensional gel electrophoresis; mass spectrometry; glycosylation; phosphorylation; protein depletion; Western blot
MeSH headings : Aged; Blood Proteins / analysis; Blotting, Western; Chromatography, High Pressure Liquid; Computational Biology; Electrophoresis, Gel, Two-Dimensional; Female; Fibrinopeptide A / analysis; Humans; Mass Spectrometry; Middle Aged; Ovarian Neoplasms / blood
topics (OpenAlex): Blood properties and coagulation; Adipokines, Inflammation, and Metabolic Diseases; Advanced Proteomics Techniques and Applications
TL;DR: This work evaluated the differentially expressed proteins in the plasma of ovarian cancer patients using 2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (P... (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Focus in Honor of James A. McCloskey, Recipient of the 2005 ASMS award for a distinguished contribution in mass spectrometry

Journal of the American Society for Mass Spectrometry, 17(10), I1–I2.

By: P. Limbach & D. Muddiman*

MeSH headings : Awards and Prizes; Biochemistry; Chemistry, Analytic; History, 20th Century; History, 21st Century; Mass Spectrometry; Nucleic Acids / analysis; Texas; Utah
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2006 article

Generation and detection of multiply-charged peptides and proteins by matrix-assisted laser desorption electrospray ionization (MALDESI) fourier transform ion cyclotron resonance mass spectrometry

Sampson, J. S., Hawkridge, A. M., & Muddiman, D. C. (2006, September 7). Journal of the American Society for Mass Spectrometry, Vol. 17, pp. 1712–1716.

By: J. Sampson n, A. Hawkridge n & D. Muddiman n

MeSH headings : Algorithms; Cyclotrons; Peptide Mapping / methods; Peptides / analysis; Proteins / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Static Electricity
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Proteomics Techniques and Applications
TL;DR: Analysis of peptides and proteins resulted in high resolving power single-acquisition FT-ICR mass spectra with average charge-states highly correlated to those obtained by nanoESI, thus, providing strong evidence that the ESI process dictates the observed charge-state distribution. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Identification of Subunit−Subunit Interactions in Bacteriophage P22 Procapsids by Chemical Cross-linking and Mass Spectrometry

Journal of Proteome Research, 5(2), 370–377.

By: S. Kang*, A. Hawkridge*, K. Johnson*, D. Muddiman* & P. Prevelige*

author keywords: bacteriophage P22; capsid interactions; chemical cross-linking; mass spectrometry
MeSH headings : Amino Acid Substitution / genetics; Bacteriophage P22 / metabolism; Capsid / metabolism; Capsid Proteins / genetics; Capsid Proteins / metabolism; Cross-Linking Reagents; Cysteine / chemistry; Cysteine / genetics; Mass Spectrometry; Models, Molecular; Protein Binding; Protein Subunits / metabolism; Threonine / chemistry; Threonine / genetics; Virus Assembly
topics (OpenAlex): Bacteriophages and microbial interactions; Enzyme Structure and Function; Protein Structure and Dynamics
TL;DR: To build a detailed structure model, the secondary structure elements of the P22 coat protein are predicted, and the prediction onto identified helical elements of cryoEM 3D reconstruction is attempted. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2006 article

Incorporation of 2,6-Di(4,4‘-dipyridyl)-9-thiabicyclo[3.3.1]nonane into Discrete 2D Supramolecules via Coordination-Driven Self-Assembly

Yang, H.-B., Das, N., Huang, F., Hawkridge, A. M., Díaz, D. D., Arif, A. M., … Stang, P. J. (2006, July 15). The Journal of Organic Chemistry, Vol. 71, pp. 6644–6647.

MeSH headings : Bridged Bicyclo Compounds / chemistry; Molecular Structure; Platinum / chemistry; Pyridines / chemistry; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared
topics (OpenAlex): Supramolecular Chemistry and Complexes; Metal-Organic Frameworks: Synthesis and Applications; Magnetism in coordination complexes
TL;DR: The synthesis and characterization of three new supramolecular complexes 6-8 (a rhomboid and two hexagons) via coordination-driven self-assembly are reported in excellent yields (>90%) and the X-ray structure of the 120 degrees tecton 2 is discussed. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Molecular Architecture via Coordination:  Self-Assembly of Nanoscale Hexagonal Metallodendrimers with Designed Building Blocks

Journal of the American Chemical Society, 128(31), 10014–10015.

MeSH headings : Dendrimers / chemistry; Metals / chemistry; Models, Molecular; Nanotechnology
topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Supramolecular Chemistry and Complexes; Advanced biosensing and bioanalysis techniques
TL;DR: The first self-assembly of nanoscale metallodendrimers that have a hexagonal cavity as a core via the directional-bonding approach is reported. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
11. Sustainable Cities and Communities (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2006 journal article

Molecular Architecture via Coordination:  Self-Assembly of Nanoscale Hexagonal Metallodendrimers with Designed Building Blocks

Journal of American Chemical Society, 128(31), 10014–10015.

By: H. Yang, N. Das, F. Huang, A. Hawkridge, D. Muddiman & P. Stang

topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: March 8, 2021

2006 article

Sub parts-per-million mass measurement accuracy of intact proteins and product ions achieved using a dual electrospray ionization quadrupole fourier transform ion cyclotron resonance mass spectrometer

Williams, D. K., Hawkridge, A. M., & Muddiman, D. C. (2006, September 19). Journal of the American Society for Mass Spectrometry, Vol. 18, pp. 1–7.

By: D. Williams n, A. Hawkridge n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Calibration; Cyclotrons; Ions; Melitten / chemistry; Molecular Sequence Data; Nanotechnology; Polymers / chemistry; Propylene Glycols / chemistry; Proteomics; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Mass Spectrometry; Ubiquitin / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: While external calibration of protein fragments resulted in absolute MMA greater than 16 ppm, internal standardization significantly improved upon the MMA of both the intact proteins and their products ions which ranged from −2.0 ppm to 1.1 ppm. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 article

Accurate mass precursor ion data and tandem mass spectrometry identify a class I human leukocyte antigen A*0201-presented peptide originating from vaccinia virus

Johnson, K. L., Ovsyannikova, I. G., Madden, B. J., Poland, G. A., & Muddiman, D. C. (2005, September 27). Journal of the American Society for Mass Spectrometry, Vol. 16, pp. 1812–1817.

MeSH headings : Amino Acid Sequence; HLA Antigens / chemistry; HLA Antigens / immunology; HeLa Cells; Humans; Immunoassay / methods; Microchemistry / methods; Molecular Sequence Data; Nucleoside-Phosphate Kinase / chemistry; Nucleoside-Phosphate Kinase / immunology; Peptides / analysis; Peptides / chemistry; Protein Precursors / analysis; Protein Precursors / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Vaccinia virus / chemistry; Vaccinia virus / immunology; Viral Proteins / analysis; Viral Proteins / chemistry
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; RNA and protein synthesis mechanisms
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Coordination-Driven Self-Assemblies with a Carborane Backbone

Journal of the American Chemical Society, 127(34), 12131–12139.

By: H. Jude*, H. Disteldorf*, S. Fischer*, T. Wedge*, A. Hawkridge*, A. Arif*, M. Hawthorne*, D. Muddiman*, P. Stang*

MeSH headings : Boranes / chemistry; Crystallography, X-Ray; Isotope Labeling; Magnetic Resonance Spectroscopy; Molecular Structure; Pyridines / chemistry; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Boron Compounds in Chemistry; Radioactive element chemistry and processing; Supramolecular Chemistry and Complexes
TL;DR: Isotopically resolved mass spectrometry data confirm the existence of the rectangle, triangle, and hexagon, and NMR data are consistent with the formation of all five assemblies. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Déjà Vu All Over Again: Skin Cap Still Contains a High-Potency Glucocorticosteroid

Archives of Dermatology, 141(6).

By: D. Swanson, M. Pittelkow, L. Benson, A. Hawkridge & D. Muddiman*

MeSH headings : Administration, Topical; Adrenal Cortex Hormones / adverse effects; Adrenal Cortex Hormones / chemistry; Adrenal Cortex Hormones / therapeutic use; Chemistry, Pharmaceutical; Dermatologic Agents / adverse effects; Dermatologic Agents / pharmacology; Dermatologic Agents / therapeutic use; Female; Humans; Male; Materials Testing; Psoriasis / diagnosis; Psoriasis / drug therapy; Risk Assessment; Sensitivity and Specificity
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Forensic Toxicology and Drug Analysis
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2005 journal article

Effect of post-excitation radius on ion abundance, mass measurement accuracy, and isotopic distributions in Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 19(7), 915–918.

By: A. Hawkridge*, A. Nepomuceno*, S. Lovik*, C. Mason* & D. Muddiman*

MeSH headings : Bradykinin / analysis; Ions; Isotopes; Reproducibility of Results; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tritium
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Isotope Analysis in Ecology
TL;DR: The findings presented herein underscore the importance of determining the optimal post-excitation radius in FT-ICR-MS to achieve high ion abundance (low limits of detection), high MMA, and valid isotopic distributions. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Evaluation of Protein Depletion Methods for the Analysis of Total-, Phospho- and Glycoproteins in Lumbar Cerebrospinal Fluid

Journal of Proteome Research, 4(3), 837–845.

By: Y. Ogata*, M. Charlesworth* & D. Muddiman*

author keywords: cerebrospinal fluid; human; two-dimensional gel electrophoresis; mass spectrometry; glycoprotein; phosphoprotein; protein depletion; low-abundance protein
MeSH headings : Adult; Aged; Cerebrospinal Fluid Proteins / analysis; Cerebrospinal Fluid Proteins / classification; Cerebrospinal Fluid Proteins / isolation & purification; Chromatography, Affinity; Electrophoresis, Gel, Two-Dimensional; Female; Fluorescent Dyes; Glycoproteins / cerebrospinal fluid; Glycoproteins / isolation & purification; Humans; Immunoglobulins; Male; Mass Spectrometry; Methods; Microspheres; Middle Aged; Phosphoproteins / cerebrospinal fluid; Phosphoproteins / isolation & purification
topics (OpenAlex): Hemoglobin structure and function; Glycosylation and Glycoproteins Research; Advanced Proteomics Techniques and Applications
TL;DR: Phospho- and glycoprotein specific fluorescent stains and mass spectrometry were used to map proteins from CSF on two-dimensional gel electropherograms after immunoaffinity based protein removal, and fifty proteins were identified from 66 spots, and 12 proteins have not been annotated in previously published 2D gels. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Gentle Protein Ionization Assisted by High-Velocity Gas Flow

Analytical Chemistry, 77(19), 6174–6183.

By: P. Yang*, R. Cooks, Z. Ouyang, A. Hawkridge & D. Muddiman*

MeSH headings : Cytochromes c / chemistry; Hydrogen-Ion Concentration; Ions / chemistry; Myoglobin / chemistry; Proteins / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Ubiquitin / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Electrohydrodynamics and Fluid Dynamics; Microfluidic and Capillary Electrophoresis Applications
TL;DR: Evidence is provided for the importance of the conditions used in the desolvation process for the preservation of the protein conformation and suggest that the conditions achieved when using high-velocity gas flows to assist droplet evaporation and ion Desolvation are much gentler than those in conventional ESI experiments. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 article

Identification of Class II HLA-DRB1*03-Bound Measles Virus Peptides by 2D-Liquid Chromatography Tandem Mass Spectrometry

Johnson, K. L., Ovsyannikova, I. G., Poland, G. A., & Muddiman, D. C. (2005, October 19). Journal of Proteome Research, Vol. 4, pp. 2243–2249.

author keywords: measles; HLA class II peptides; 2D-LC; tandem mass spectrometry; MS/MS
MeSH headings : Amino Acid Sequence; Cancer Vaccines / chemistry; Cations; Chromatography; Chromatography, Ion Exchange; Chromatography, Liquid / methods; Databases, Factual; Databases, Protein; HLA Antigens / chemistry; HLA-DR Antigens / genetics; HLA-DRB1 Chains; Hemagglutinins / chemistry; Humans; Leukocytes, Mononuclear / metabolism; Mass Spectrometry / methods; Measles Vaccine / chemistry; Measles virus / genetics; Molecular Sequence Data; Nucleocapsid Proteins / chemistry; Peptides / chemistry; Peptides / genetics; Phosphoproteins / chemistry; Proteomics / methods; Sequence Homology, Amino Acid; Time Factors
topics (OpenAlex): Virology and Viral Diseases; vaccines and immunoinformatics approaches; Microbial infections and disease research
TL;DR: Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Leveling response factors in the electrospray ionization process using a heated capillary interface

Journal of the American Society for Mass Spectrometry, 16(5), 772–778.

By: J. Frahm*, D. Muddiman* & M. Burke*

MeSH headings : Capillary Action; DNA / analysis; DNA / chemistry; Equipment Design; Equipment Failure Analysis; Hot Temperature; Humans; Specimen Handling / instrumentation; Specimen Handling / methods; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Electrohydrodynamics and Fluid Dynamics; Microfluidic and Capillary Electrophoresis Applications
TL;DR: The implementation of a heated electrospray source was used to denature amplicons containing 7-deaza purines, which can be used to facilitate sequencing by mass spectrometry. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Mapping Sites of Protein Phosphorylation by Mass Spectrometry Utilizing a Chemical-Enzymatic Approach:  Characterization of Products from α-S1Casein Phosphopeptides

Journal of Proteome Research, 4(2), 424–434.

By: D. McCormick*, M. Holmes*, D. Muddiman* & B. Madden*

author keywords: chemical-enzymatic; phosphorylation; 2-aminoethanethiol; isotope dilution mass spectrometry; phosphoproteins; phosphopeptides; quantification
MeSH headings : Amino Acid Sequence; Animals; Caseins / metabolism; Cattle; Molecular Sequence Data; Peptide Fragments / metabolism; Phosphopeptides / metabolism; Phosphorylation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Enzyme Structure and Function; Mass Spectrometry Techniques and Applications
TL;DR: ESI-TOF mass spectrometry was used to characterize products from the chemical-enzymatic cleavage of specific phosphorylation sites in bovine alpha-S1 casein and synthetic phosphopeptides containing substitutions at a single phosphorylated site. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2005 journal article

Normalization of relative peptide ratios derived from in-gel digests: applications to protein variant analysis at the peptide level

Rapid Communications in Mass Spectrometry, 19(19), 2871–2877.

By: H. Bergen*, D. Muddiman*, J. O'Brien* & J. Hoyer*

MeSH headings : Algorithms; Chromatography, High Pressure Liquid / methods; Gas Chromatography-Mass Spectrometry / methods; Gas Chromatography-Mass Spectrometry / standards; Humans; Osmotic Fragility; Peptide Mapping / methods; Peptide Mapping / standards; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Spectrin / analysis; Spectrin / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Forensic and Genetic Research
TL;DR: A strategy was developed to normalize for sample variability due to ionization efficiency, solvent effects, digestion and extraction efficiency by utilizing multiple isotopically labeled internal standards for multiple peptides derived from the same protein. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Nucleic Acid Analysis by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at the Beginning of the Twenty-First Century

Current Pharmaceutical Design, 11(20), 2593–2613.

By: J. Frahm* & D. Muddiman*

author keywords: FT-ICR; Fourier transform ion cyclotron resonance; mass spectrometry; DNA; RNA; PNA
MeSH headings : Nucleic Acids / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Electrospray Ionization / trends; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / trends; Spectroscopy, Fourier Transform Infrared / methods; Spectroscopy, Fourier Transform Infrared / trends; Spectrum Analysis / methods; Spectrum Analysis / trends; Technology, Pharmaceutical / instrumentation; Technology, Pharmaceutical / methods; Technology, Pharmaceutical / trends
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: A diverse range of studies including fundamental studies of nucleic acid properties, improvements in sample clean up and preparation, better methods to obtain higher mass measurement accuracy, analysis of noncovalent complexes, tandem mass spectrometry, and characterization of peptide nucleic acids are presented. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2005 journal article

Primary Trabecular Meshwork Cells Incubated in Human Aqueous Humor Differ from Cells Incubated in Serum Supplements

Investigative Opthalmology & Visual Science, 46(8), 2848.

By: M. Fautsch, K. Howell, A. Vrabel, M. Charlesworth*, D. Muddiman* & D. Johnson

MeSH headings : Adult; Aged; Aged, 80 and over; Aqueous Humor / physiology; Ascorbic Acid / metabolism; Ascorbic Acid / pharmacology; Blotting, Western; Cell Culture Techniques; Cell Proliferation; Culture Media; Cytoskeletal Proteins / metabolism; Dexamethasone / pharmacology; Electrophoresis, Gel, Two-Dimensional; Eye Proteins / metabolism; Female; Glycoproteins / metabolism; Humans; Infant; Male; Middle Aged; Serum; Tissue Inhibitor of Metalloproteinase-1 / metabolism; Trabecular Meshwork / cytology; Trabecular Meshwork / drug effects; Trabecular Meshwork / metabolism
topics (OpenAlex): Proteoglycans and glycosaminoglycans research; Fibroblast Growth Factor Research; Calpain Protease Function and Regulation
TL;DR: Addition of human aqueous humor rather than FBS to trabecular monolayer cell cultures triggers significant changes in cellular and molecular characteristics. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2005 journal article

Protein expression profiling of CLL B cells using replicate off-line strong cation exchange chromatography and LC–MS/MS

Journal of Chromatography B, 819(1), 33–39.

By: D. Barnidge*, R. Tschumper*, D. Jelinek*, D. Muddiman* & N. Kay*

author keywords: CLL; 2D-LC-MS/MS; B cell; expression profiling; off-line SCX; reproducibility
MeSH headings : Antigens, CD / isolation & purification; B-Lymphocytes / metabolism; Blood Proteins / isolation & purification; Cell Fractionation; Cell Membrane / chemistry; Chromatography, Ion Exchange / methods; Chromatography, Liquid / methods; Gene Expression Profiling / methods; Humans; Leukemia, Lymphocytic, Chronic, B-Cell / metabolism; Mass Spectrometry / methods; Neoplasm Proteins / blood
topics (OpenAlex): Chronic Lymphocytic Leukemia Research; Monoclonal and Polyclonal Antibodies Research; Glycosylation and Glycoproteins Research
TL;DR: Examination of crude membranes from B cells derived from a patient with chronic lymphocytic leukemia to examine the protein expression profile of CLL B cells shows an overlap of 64% between proteins identified in replicate runs was achieved for this system. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2005 journal article

Quantitative Protein Expression Analysis Of CLL B Cells from Mutated and Unmutated IgVHSubgroups Using Acid-Cleavable Isotope-Coded Affinity Tag Reagents

Journal of Proteome Research, 4(4), 1310–1317.

By: D. Barnidge*, D. Jelinek*, D. Muddiman* & N. Kay*

author keywords: CLL; B cell; quantitative comparison; clCAT; UM-CLL; M-CLL; linear ion trap mass spectrometer
MeSH headings : B-Lymphocytes / chemistry; B-Lymphocytes / immunology; Cell Fractionation; Genes, Immunoglobulin; Humans; Immunoglobulin Heavy Chains / genetics; Immunoglobulin Heavy Chains / immunology; Immunoglobulin Variable Region / genetics; Immunoglobulin Variable Region / immunology; Isotope Labeling / methods; Leukemia, Lymphocytic, Chronic, B-Cell / immunology; Mass Spectrometry / methods; Mutation; Proteome / analysis
topics (OpenAlex): Chronic Lymphocytic Leukemia Research; Glycosylation and Glycoproteins Research; Monoclonal and Polyclonal Antibodies Research
TL;DR: Stable isotope labeling and mass spectrometry can complement 2D gel electrophoresis and gene microarray technologies for identifying putative and perhaps unique prognostic markers in CLL. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2005 journal article

Quantitative mass spectral evidence for the absence of circulating brain natriuretic peptide (BNP-32) in severe human heart failure

Proceedings of the National Academy of Sciences, 102(48), 17442–17447.

By: A. Hawkridge*, D. Heublein*, H. Bergen*, A. Cataliotti*, J. Burnett* & D. Muddiman n

author keywords: biomarkers; electrospray ionization; Fourier transform-ion cyclotron resonance MS; immunoaffinity purification; targeted proteomics
MeSH headings : Chromatography, Liquid; Female; Heart Failure / metabolism; Humans; Isotope Labeling; Male; Mass Spectrometry; Natriuretic Peptide, Brain / blood; Natriuretic Peptide, Brain / isolation & purification
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Advanced Proteomics Techniques and Applications
TL;DR: An immunoaffinity purification assay is developed and used to isolate endogenous BNP-32 from New York Heart Association class IV patient plasma for subsequent analysis by nano-liquid chromatography (LC) electrospray ionization Fourier transform ion cyclotron resonance (FT-ICR) MS. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Reproducibility of Retention Time using a Splitless nanoLC Coupled to an ESI-FTICR Mass Spectrometer

Journal of Biomolecular Techniques, 16, 4, 414–422.

By: D. Muddiman, C. Mason & K. Johnson

Source: NC State University Libraries
Added: March 17, 2021

2005 journal article

Statistical Evaluation of Internal and External Mass Calibration Laws Utilized in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Analytical Chemistry, 77(8), 2406–2414.

By: D. Muddiman* & A. Oberg*

MeSH headings : Calibration; Cyclotrons; Data Interpretation, Statistical; Fourier Analysis; Polymers / analysis; Polymers / chemistry; Propylene Glycols / analysis; Propylene Glycols / chemistry; Quaternary Ammonium Compounds / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: The statistical evaluation of two common and three new calibration laws utilized in Fourier transform ion cyclotron resonance mass spectrometry are presented and the number of calibrant ions as well as their required m/z range is investigated in order to successfully achieve high MMA. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2004 journal article

A method for calculating 16o/18o peptide ion ratios for the relative quantification of proteomes

Journal of the American Society for Mass Spectrometry, 15(4), 437–445.

By: K. Johnson* & D. Muddiman*

MeSH headings : Amino Acid Sequence; Animals; Apoproteins / analysis; Apoproteins / chemistry; Cattle; Mass Spectrometry; Molecular Sequence Data; Oxygen Isotopes; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Serum Albumin / analysis; Serum Albumin / chemistry; Spectroscopy, Fourier Transform Infrared; Transferrin / analysis; Transferrin / chemistry
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The data demonstrate that for biomarker discovery, it is advantageous to label the proteome representing the disease state with 18O; and the method is not sensitive to variations in 18O1/18O2 ratio. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2004 journal article

Absolute Quantification of the Model Biomarker Prostate-Specific Antigen in Serum by LC−MS/MS Using Protein Cleavage and Isotope Dilution Mass Spectrometry

Journal of Proteome Research, 3(3), 644–652.

By: D. Barnidge*, M. Goodmanson*, G. Klee* & D. Muddiman*

author keywords: LC-MS/MS; absolute quantification; serum; biomarker; prostate-specific antigen; protein
MeSH headings : Humans; Isotope Labeling; Male; Mass Spectrometry; Peptide Fragments / chemistry; Prostate-Specific Antigen / chemistry; Protein Processing, Post-Translational; Serum / chemistry; Trypsin / chemistry; Urea / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: absolute quantification can be performed on the model biomarker PSA introduced into denatured serum when analyzed by LC-MS/MS, and concerns still exist regarding sensitivity compared to existing immunoassays as well as the reproducibility of PC-IDMS performed in different matrixes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2004 conference paper

Accurate and Expedited Diagnosis of Amyloidotic Transthyretin Neuropathy: Proteomic and Genomic Approach

Annals of Neurology, 56(S8), 29.

By: C. Klein, C. Kim, P. Dyck, S. Zeldenrust, H. Bergen, J. O'Brien, A. Nepomuceno, M. Butz ...

Source: NC State University Libraries
Added: March 17, 2021

2004 journal article

Analysis of the Low Molecular Weight Fraction of Serum by LC-Dual ESI-FT-ICR Mass Spectrometry:  Precision of Retention Time, Mass, and Ion Abundance

Analytical Chemistry, 76(17), 5097–5103.

By: K. Johnson*, C. Mason*, D. Muddiman* & J. Eckel*

MeSH headings : Algorithms; Biomarkers, Tumor / blood; Chromatography, Liquid / standards; Female; Humans; Ions / blood; Molecular Weight; Ovarian Neoplasms / blood; Reproducibility of Results; Serum / chemistry; Software; Spectrometry, Mass, Electrospray Ionization / standards
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical Chemistry and Chromatography
TL;DR: These measures of analytical reproducibility establish a statistical rationale for differentiating healthy and disease patient populations for the elucidation of biomarkers in the low molecular fraction of serum. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2004 journal article

Analytical Performance of a Venturi Device Integrated into an Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Analysis of Nucleic Acids

Analytical Chemistry, 76(14), 4118–4122.

By: A. Hawkridge*, L. Zhou*, M. Lee* & D. Muddiman*

MeSH headings : Cyclotrons; Fourier Analysis; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Oligonucleotides / analysis; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Analytical Chemistry and Chromatography
TL;DR: A voltage-assisted venturi device modeled after an industrial air amplifier was used to improve the ion transmission efficiency of a 16.2 kDa oligonucleotide and a 53-mer PCR product in the high-pressure region between an electrospray ionization emitter and the sampling orifices of two Fourier transform ion cyclotron resonance mass spectrometers (FT-ICR-MS). (via Semantic Scholar)
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UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2004 journal article

Androgens Negatively Regulate Forkhead Transcription Factor FKHR (FOXO1) through a Proteolytic Mechanism in Prostate Cancer Cells

Journal of Biological Chemistry, 279(14), 13866–13877.

By: H. Huang*, D. Muddiman* & D. Tindall*

MeSH headings : Amino Acid Sequence; Androgens / pharmacology; Apoptosis / drug effects; Apoptosis / physiology; Cell Line, Tumor / cytology; Cell Line, Tumor / drug effects; Cell Line, Tumor / physiology; Cell Nucleus / metabolism; Cysteine Endopeptidases / metabolism; Forkhead Box Protein O1; Forkhead Transcription Factors; Gene Expression Regulation, Neoplastic / drug effects; Humans; Male; Metribolone / pharmacology; Molecular Sequence Data; Mutagenesis; Prostatic Neoplasms; Protease Inhibitors / pharmacology; RNA, Messenger / analysis; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation / drug effects; Transfection
topics (OpenAlex): FOXO transcription factor regulation; Signaling Pathways in Disease; Heat shock proteins research
TL;DR: This work demonstrates that forkhead transcription factor FKHR (FOXO1)-induced death of LNCaP cells was blocked by a synthetic androgen R1881, and suggests that androgens induce increased activity of an acidic cysteine protease, which in turn cleaves FK HR. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2004 journal article

Derivatives of pentamidine designed to target the Leishmania lipophosphoglycan

Tetrahedron Letters, 46(4), 695–698.

By: K. Kramp*, K. DeWitt*, J. Flora*, D. Muddiman*, K. Slunt* & T. Houston*

author keywords: boronate drug targeting; pentamidine; Leishmania
topics (OpenAlex): Research on Leishmaniasis Studies; Trypanosoma species research and implications; Helminth infection and control
TL;DR: Pentamidine has been modified with boronic acids so that it might bind more selectively to the phosphodisaccharide repeating unit of the LPG, which could serve to target the drug to the protozoan surface and increase its efficacy in vivo. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2004 journal article

Detection of Genetic Variants of Transthyretin by Liquid Chromatography–Dual Electrospray Ionization Fourier-Transform Ion-Cyclotron-Resonance Mass Spectrometry

Clinical Chemistry, 50(9), 1535–1543.

By: A. Nepomuceno*, C. Mason, D. Muddiman*, H. Bergen & S. Zeldenrust*

MeSH headings : Amyloidosis, Familial / genetics; Gas Chromatography-Mass Spectrometry / instrumentation; Gas Chromatography-Mass Spectrometry / methods; Humans; Point Mutation; Prealbumin / chemistry; Prealbumin / genetics; Sequence Analysis, DNA; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Enzyme Structure and Function
TL;DR: FT-ICR mass spectrometry has sufficient mass accuracy to identify genetic variants of immunoaffinity-purified TTR and discovered and characterized a previously unreported cis double mutation with a mass only 2 Da different from wild-type TTR. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2004 journal article

Discovery of Ovarian Cancer Biomarkers in Serum Using NanoLC Electrospray Ionization TOF and FT-ICR Mass Spectrometry

Disease Markers, 19(4-5), 239–249.

By: H. Bergen*, G. Vasmatzis*, W. Cliby*, K. Johnson*, A. Oberg* & D. Muddiman*

MeSH headings : Amino Acid Sequence; Biomarkers, Tumor / blood; Chromatography; Female; Humans; Middle Aged; Molecular Sequence Data; Nanotechnology; Neoplasm Staging; Ovarian Neoplasms / blood; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Protein purification and stability
TL;DR: A set of protocols for the isolation and identification of serum biomarkers for ovarian cancer that exist in the low molecular weight serum fraction is delineated and one of these contains the sequence of fibrinopeptide-A known to be elevated in many types of cancer including ovarian cancer. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2004 journal article

Evaluation of a Cleavable Stable Isotope Labeled Synthetic Peptide for Absolute Protein Quantification Using LC−MS/MS

Journal of Proteome Research, 3(3), 658–661.

By: D. Barnidge*, G. Hall*, J. Stocker* & D. Muddiman*

author keywords: LC-MS/MS; absolute quantification; biomarker; cleavable internal standard
MeSH headings : Chromatography, Liquid; Isotope Labeling; Mass Spectrometry; Peptides / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Ion-surface interactions and analysis
TL;DR: The work presented here evaluates the use of tryptic cleavage sites incorporated into the internal standard synthetic peptide in an attempt to create an internal standard that has cleavage characteristics more similar to the protein being quantified. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2004 journal article

Identification of Transthyretin Variants by Sequential Proteomic and Genomic Analysis

Clinical Chemistry, 50(9), 1544–1552.

By: H. Bergen*, S. Zeldenrust, M. Butz, D. Snow, P. Dyck, P. Dyck, C. Klein, J. O’Brien, S. Thibodeau, D. Muddiman*

MeSH headings : Amyloidosis, Familial / diagnosis; Amyloidosis, Familial / genetics; DNA / chemistry; DNA / genetics; Gas Chromatography-Mass Spectrometry / instrumentation; Gas Chromatography-Mass Spectrometry / methods; Genomics / methods; Genotype; Humans; Polymerase Chain Reaction; Polymorphism, Genetic; Prealbumin / chemistry; Prealbumin / genetics; Proteomics / methods; Sequence Analysis, DNA; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Amyloidosis: Diagnosis, Treatment, Outcomes; Endoplasmic Reticulum Stress and Disease; Trace Elements in Health
TL;DR: This method correctly identified all previously known variants and also identified a novel variant, Val94Ala, which allows screening for the familial forms of amyloidosis in a broad patient population in a timely fashion. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2004 journal article

Informed use of proteolytic inhibitors in biomarker discovery

Rapid Communications in Mass Spectrometry, 18(9), 1001–1002.

By: H. Bergen*, M. Klug*, M. Bolander* & D. Muddiman*

MeSH headings : Biomarkers / analysis; Combinatorial Chemistry Techniques; Drug Design; Prealbumin / analysis; Protease Inhibitors / chemistry; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2004 journal article

Utility of accurate monoisotopic mass measurements to confidently identify lambda exonuclease generated single-stranded amplicons containing 7-deaza analogs by electrospray ionization FT-ICR mass spectrometry

International Journal of Mass Spectrometry, 234(1-3), 79–87.

By: J. Frahm*, C. Mason & D. Muddiman*

topics (OpenAlex): Genomics and Phylogenetic Studies; Mass Spectrometry Techniques and Applications; Molecular Biology Techniques and Applications
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2003 journal article

1H NMR and electrospray mass spectrometry of the mono-ionized bis(2,2′-bis(4,5-dimethylimidazole)chloronitrosylruthenium(II) complex ([Ru(NO)Cl(LH2)2]+, LH2=2,2′-bis(4,5-dimethylimidazole)

Inorganica Chimica Acta, 343, 317–328.

By: T. Stringfield*, K. Somayajula*, D. Muddiman*, J. Flora* & R. Shepherd*

author keywords: ruthenium complexes; nitrosyl complexes; imidazole complexes; electrospray mass spectrometry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Molecular Sensors and Ion Detection; Analytical Chemistry and Chromatography
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2003 journal article

Advantages of Thermococcus kodakaraenis (KOD) DNA Polymerase for PCR-mass spectrometry based analyses

Journal of the American Society for Mass Spectrometry, 14(6), 601–604.

By: L. Benson*, A. Null* & D. Muddiman*

MeSH headings : DNA-Directed DNA Polymerase / chemistry; Fourier Analysis; Humans; Polymerase Chain Reaction / methods; Spectrometry, Mass, Electrospray Ionization; Taq Polymerase / chemistry; Thermococcus / enzymology; von Willebrand Factor / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Genomics and Phylogenetic Studies; Ion-surface interactions and analysis
TL;DR: KOD DNA polymerase demonstrated a 2- to 3-fold increase in PCR product formation compared to Pfu or Taq, respectively, and generated blunt-ended PCR product which allows facile interpretation of the mass spectrum. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Cancer Biomarkers: How Proteomics is Leading to the Discovery of New Markers

Clinical Laboratory News, 29, 12–16.

By: D. Muddiman, W. Cliby & H. Bergen

Source: NC State University Libraries
Added: March 17, 2021

2003 journal article

Coordination-Driven Self-Assembly of Supramolecular Cages:  Heteroatom-Containing and Complementary Trigonal Prisms

Journal of the American Chemical Society, 125(32), 9647–9652.

By: Y. Kryschenko*, S. Seidel*, D. Muddiman*, A. Nepomuceno* & P. Stang*

MeSH headings : Anthracenes / chemical synthesis; Anthracenes / chemistry; Benzene Derivatives / chemical synthesis; Benzene Derivatives / chemistry; Cross-Linking Reagents / chemical synthesis; Cross-Linking Reagents / chemistry; Crystallography, X-Ray; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; Organosilicon Compounds / chemical synthesis; Organosilicon Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Supramolecular Chemistry and Complexes; Crystallography and molecular interactions; Molecular Sensors and Ion Detection
TL;DR: The self-assembly of three nanoscopic prisms of approximate size 1 x 4 nm is reported, which completes a pair of nanoscopic complementary 3-D structures. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 17(15), 1714–1722.

By: A. Null* & D. Muddiman*

MeSH headings : Humans; Molecular Weight; Nucleic Acids / analysis; Polymerase Chain Reaction; Proteins / analysis; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Repeat Sequences / genetics
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Isotope Analysis in Ecology
TL;DR: A practical correction factor is developed that is applied to the ToC measurement to largely remove the systematic bias introduced by nature and accurately predicts resolving power achievable for Fourier transform ion cyclotron resonance (FT-ICR) mass analysis of large molecules. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Determination of the relative energies of activation for the dissociation of aromatic versus aliphatic phosphopeptides by ESI-FTICR-MS and IRMPD

Journal of the American Society for Mass Spectrometry, 15(1), 121–127.

By: J. Flora* & D. Muddiman*

MeSH headings : Peptide Mapping / methods; Phosphopeptides / analysis; Phosphopeptides / chemistry; Protein Structure, Secondary; Serine / analysis; Serine / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Thermodynamics; Tyrosine / analysis; Tyrosine / chemistry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Enzyme Structure and Function
TL;DR: The data presented in this manuscript support the theory that for phosphopeptides, the phosphate moiety’s P-O stretch is in direct resonance with the infrared laser, thus increasing the relative absorptivity of the modified species. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2003 journal article

Dual Electrospray Ionization Source for Confident Generation of Accurate Mass Tags Using Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Analytical Chemistry, 75(14), 3411–3418.

By: A. Nepomuceno*, D. Muddiman*, H. Bergen*, J. Craighead*, M. Burke*, P. Caskey*, J. Allan*

MeSH headings : Amino Acid Sequence; Apoproteins / chemistry; Chromatography, Liquid; Cyclotrons; Mass Spectrometry; Molecular Sequence Data; Myoglobin / chemistry; Proteins / chemistry; Proteomics / instrumentation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectroscopy, Fourier Transform Infrared / instrumentation
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: It is argued that the most effective way to account for space-charge effects is to introduce an internal mass calibrant (IMC) using a dual electrospray ionization (ESI) source where the IMC is added from a separate ESI emitter. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Enzymatic strategies for the characterization of nucleic acids by electrospray ionization mass spectrometry

Rapid Communications in Mass Spectrometry, 17(24), 2699–2706.

By: A. Null*, L. Benson* & D. Muddiman*

MeSH headings : Exodeoxyribonucleases / chemistry; Isotope Labeling / methods; Nucleic Acids / analysis; Nucleic Acids / chemistry; Oligonucleotides / analysis; Oligonucleotides / chemistry; Polymerase Chain Reaction / methods; Polymorphism, Single Nucleotide; Reproducibility of Results; Restriction Mapping / methods; Sensitivity and Specificity; Sequence Alignment / methods; Sequence Analysis, DNA / methods; Spectrometry, Mass, Electrospray Ionization / methods; Viral Proteins
topics (OpenAlex): Mass Spectrometry Techniques and Applications; RNA and protein synthesis mechanisms; Enzyme Structure and Function
TL;DR: This work describes an eclectic array of enzymatic strategies invoked in order to detect single-nucleotide polymorphisms by ESI-MS, although other applications may be envisioned. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2003 journal article

Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides

Journal of Virology, 78(1), 42–51.

By: I. Ovsyannikova*, K. Johnson*, D. Muddiman*, R. Vierkant* & G. Poland*

MeSH headings : Alleles; Cytokines / biosynthesis; Cytokines / genetics; HLA-DR Antigens / chemistry; HLA-DR Antigens / metabolism; HLA-DRB1 Chains; Humans; Interferon-gamma / biosynthesis; Interferon-gamma / genetics; Interleukin-4 / biosynthesis; Interleukin-4 / genetics; Measles / immunology; Measles / prevention & control; Measles virus / immunology; Measles virus / metabolism; Measles-Mumps-Rubella Vaccine / administration & dosage; Nucleocapsid Proteins; Nucleoproteins / chemistry; Nucleoproteins / immunology; Nucleoproteins / metabolism; Peptides / chemical synthesis; Peptides / chemistry; Peptides / immunology; Peptides / metabolism; T-Lymphocytes / immunology; Viral Proteins / chemistry; Viral Proteins / immunology; Viral Proteins / metabolism
topics (OpenAlex): Virology and Viral Diseases; Respiratory viral infections research; Immunodeficiency and Autoimmune Disorders
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Implications of Hydrophobicity and Free Energy of Solvation for Characterization of Nucleic Acids by Electrospray Ionization Mass Spectrometry

Analytical Chemistry, 75(6), 1331–1339.

By: A. Null*, A. Nepomuceno* & D. Muddiman*

MeSH headings : Deoxyribonucleotides / analysis; Gene Frequency; Hydrophobic and Hydrophilic Interactions; Nucleic Acids / chemistry; Oligonucleotides / analysis; Polymorphism, Single Nucleotide; Solvents; Spectrometry, Mass, Electrospray Ionization / methods; Thermodynamics
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: Investigation of the contributions of hydrophobicity and free energy of solvation to relative signal intensities in ESI-MS spectra of nucleic acids found a trend in signal intensity for deoxyribonucleotide triphosphates, oligonucleotides, and PCR amplicons was observed that was consistent with the model. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Mass spectral determination of skeletal/cardiac actin isoform ratios in cardiac muscle

Rapid Communications in Mass Spectrometry, 17(13), 1467–1471.

By: H. Bergen*, K. Ajtai*, T. Burghardt*, A. Nepomuceno* & D. Muddiman*

MeSH headings : Actins / analysis; Animals; Cattle; Mass Spectrometry; Muscle, Skeletal / chemistry; Myocardium / chemistry; Protein Isoforms / analysis; Rabbits
topics (OpenAlex): Cardiomyopathy and Myosin Studies; Cardiovascular Effects of Exercise; Mass Spectrometry Techniques and Applications
TL;DR: A mass spectrometric technique to measure the ratio of skeletal to cardiac actin and a new dual electrospray ionization source was employed to determine accurate masses of the alpha-skeletal and alpha-cardiac actins. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2003 journal article

Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood

Virology, 312(2), 495–506.

By: I. Ovsyannikova*, K. Johnson*, S. Naylor*, D. Muddiman* & G. Poland*

author keywords: measles-derived peptide; HLA-DRB1*03; measles virus; mass spectrometry; lymphocyte proliferation
MeSH headings : Adolescent; Amino Acid Sequence; Antigen Presentation; Antigens, Viral / analysis; Antigens, Viral / chemistry; Antigens, Viral / immunology; Antigens, Viral / metabolism; Child; Female; HLA-DR Antigens / immunology; HLA-DR Antigens / metabolism; HLA-DRB1 Chains; Humans; Male; Mass Spectrometry; Measles Vaccine / immunology; Measles virus / chemistry; Measles virus / metabolism; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / immunology; Peptides / metabolism; T-Lymphocytes / immunology
topics (OpenAlex): Virology and Viral Diseases; vaccines and immunoinformatics approaches; Immunodeficiency and Autoimmune Disorders
TL;DR: This data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

CEPH family 1362 STR database: An online resource for characterization of PCR products using electrospray ionization mass spectrometry

Journal of the American Society for Mass Spectrometry, 13(1), 89–90.

By: A. Null* & D. Muddiman*

MeSH headings : DNA, Complementary / chemistry; Databases, Genetic; Genetic Markers; Humans; Internet; Polymerase Chain Reaction / methods; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Repeat Sequences / genetics
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Molecular Biology Techniques and Applications
TL;DR: An online database has been established in order to validate electrospray ionization mass spectrometry (ESI-MS) for genotyping and to publicize the procedures developed in the laboratory for the characterization of PCR products by ESI-MS. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

Dual-micro-ESI source for precise mass determination on a quadrupole time-of-flight mass spectrometer for genomic and proteomic applications

Analytical and Bioanalytical Chemistry, 373(7), 538–546.

By: J. Flora*, A. Null* & D. Muddiman*

author keywords: electrospray; time-of-flight; mass spectrometry; internal calibration; accurate mass
MeSH headings : Amino Acid Sequence; Calibration; Caseins / chemistry; Caseins / metabolism; Genomics / methods; Ions; Molecular Sequence Data; Phosphoproteins / chemistry; Phosphoproteins / metabolism; Polymerase Chain Reaction; Proteome; Sensitivity and Specificity; Sequence Analysis, Protein; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Trypsin / metabolism
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Analytical chemistry methods development
TL;DR: In this report, the dual-sprayer is demonstrated in the negative-ion mode for internal calibration of polymerase chain reaction (PCR) amplicons generated from synthetic and genomic templates as well as a proteolytic digest of a naturally phosphorylated protein. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

Evaluation of sample preparation techniques for mass measurements of PCR products using ESI-FT-ICR mass spectrometry

Journal of the American Society for Mass Spectrometry, 13(4), 338–344.

By: A. Null*, L. George* & D. Muddiman*

MeSH headings : 2-Propanol / chemistry; 2-Propanol / isolation & purification; DNA / chemistry; Ethanol / chemistry; Ethanol / isolation & purification; Indicators and Reagents; Microdialysis; Polymerase Chain Reaction / methods; Solvents; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Molecular Biology Techniques and Applications
TL;DR: Alternative cleanup techniques that are compatible with automation are explored here with the intent of expanding the bottleneck that exists between the production of PCR products and analysis by electrospray ionization mass spectrometry (ESI-MS). (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2002 journal article

Gas-Phase Ion Unimolecular Dissociation for Rapid Phosphopeptide Mapping by IRMPD in a Penning Ion Trap:  An Energetically Favored Process

Journal of the American Chemical Society, 124(23), 6546–6547.

By: J. Flora* & D. Muddiman*

MeSH headings : Amino Acid Sequence; Lasers; Peptide Mapping / methods; Phosphopeptides / chemistry; Receptor, Insulin / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Thermodynamics
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Advanced Proteomics Techniques and Applications
TL;DR: This research demonstrates that the energy of activation for dissociation of the phosphopeptides is lower than that of the unmodified analogues providing the basis for the success of this technique, and IRMPD of ions in a Penning ion trap is an ideal platform for rapid phosphopeptic mapping. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

In celebration of the 70th birthday of Dr David M. Hercules

Analytical and Bioanalytical Chemistry, 373(7), 517–518.

By: J. Gardella*, F. Lytle* & D. Muddiman*

MeSH headings : Chemistry, Analytic / history; History, 20th Century; History, 21st Century; Spectrum Analysis; United States
topics (OpenAlex): Radioactive Decay and Measurement Techniques; Mitochondrial Function and Pathology; Advances in Oncology and Radiotherapy
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

Memoirs, Hercules lineage, students, visiting scientists and staff

Analytical and Bioanalytical Chemistry, 373(7), 669–675.

By: J. Gardella*, F. Lytle & D. Muddiman*

topics (OpenAlex):
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

Solution composition and thermal denaturation for the production of single-stranded PCR amplicons: Piperidine-induced destabilization of the DNA duplex?

Journal of the American Society for Mass Spectrometry, 13(3), 232–240.

By: J. Mangrum*, J. Flora* & D. Muddiman*

MeSH headings : Cyclotrons; DNA / chemistry; DNA / drug effects; DNA, Single-Stranded / chemistry; Fourier Analysis; Hot Temperature; Humans; Nucleic Acid Denaturation / drug effects; Oligonucleotides / chemistry; Piperidines / pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Analytical Chemistry and Chromatography
TL;DR: The volatile base piperidine, present at 20 mM concentrations in the electrospray solution, was found to play a critical role in the formation of single-stranded species at the higher aqueous percentages and a duplex destabilization mechanism has been proposed. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2002 journal article

Tailoring the gas-phase dissociation and determining the relative energy of activation for dissociation of 7-deaza purine modified oligonucleotides containing a repeating motif

International Journal of Mass Spectrometry, 219(1), 139–150.

By: J. Hannis* & D. Muddiman*

topics (OpenAlex): DNA and Nucleic Acid Chemistry; Advanced biosensing and bioanalysis techniques; RNA and protein synthesis mechanisms
TL;DR: 7-Deaza purine modified oligonucleotides with a repeating motif have been sequenced by slow heating methods illustrating the ability to alter the preferred unimolecular decomposition pathway and limited the number of product ions per repeat unit resulting in an increased signal-to-noise ratio for the tandem mass spectrometry data. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2001 journal article

Complete sequencing of mono—deprotonated peptide nucleic acids by sustained off-resonance irradiation collision—induced dissociation

Journal of the American Society for Mass Spectrometry, 12(7), 805–809.

By: J. Flora* & D. Muddiman*

MeSH headings : Calibration; Chemical Phenomena; Chemistry, Physical; Mass Spectrometry; Peptide Nucleic Acids / chemistry; Sequence Analysis, DNA
topics (OpenAlex): Mass Spectrometry Techniques and Applications; DNA and Nucleic Acid Chemistry; Chemical Synthesis and Analysis
TL;DR: Complete peptide nucleic acids (PNAs) sequence information is obtained from the unimolecular decomposition of singly-charged PNA oligomers in the negative-ion mode using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry and sustained off-resonance irradiation collision induced dissociation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2001 journal article

Detection of double-stranded PCR amplicons at the attomole level electrosprayed from low nanomolar solutions using FT-ICR mass spectrometry

Fresenius' Journal of Analytical Chemistry, 369(3-4), 246–251.

By: J. Hannis* & D. Muddiman*

MeSH headings : Alleles; Cyclotrons; DNA / chemistry; DNA / genetics; Fourier Analysis; Homozygote; Humans; Mass Spectrometry / methods; Polymerase Chain Reaction / methods; Sensitivity and Specificity; Solutions; Spectrometry, Mass, Electrospray Ionization / methods; Spectrophotometry / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Biosensors and Analytical Detection
TL;DR: A remarkable sensitivity for large PCR amplicons will dramatically improve the ability of electrospray ionization mass spectrometry to address important genetic questions for low copy number genes or when the amount of initial template is limited; the latter issue is commonly encountered in DNA forensics. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2001 journal article

Genotyping of Simple and Compound Short Tandem Repeat Loci Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Analytical Chemistry, 73(18), 4514–4521.

By: A. Null*, J. Hannis* & D. Muddiman*

MeSH headings : Cyclotrons; Genotype; Humans; Microsatellite Repeats; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Molecular Biology Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: The utility of electrospray ionization Fourier transform ion cyclotron resonance (ESI-FIICR) mass spectrometry as a new approach for genotyping short tandem repeats (STRs) is demonstrated and is a powerful approach to characterize both simple and compound STRs beyond the capabilities of electrophoretic technologies. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2001 journal article

Genotyping short tandem repeats using flow injection and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 15(5), 348–350.

By: J. Hannis* & D. Muddiman*

MeSH headings : Alleles; Cyclotrons; Flow Injection Analysis; Fourier Analysis; Genotype; Homozygote; Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Tandem Repeat Sequences
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Microfluidic and Capillary Electrophoresis Applications
TL;DR: Characterizing polymerase chain reaction (PCR) amplicons has been accomplished for the first time using flow injection analysis coupled to electrospray ionization mass spectrometry (ESI-MS) to illustrate the compatibility of ESI-MS for genotyping short tandem repeat sequences and the potential for high-throughput genotypesing of large PCR amplicons. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2001 journal article

High-Mass Accuracy of Product Ions Produced by SORI-CID Using a Dual Electrospray Ionization Source Coupled with FTICR Mass Spectrometry

Analytical Chemistry, 73(6), 1247–1251.

By: J. Flora*, J. Hannis* & D. Muddiman*

MeSH headings : Cyclotrons; Fourier Analysis; Oligonucleotides / analysis; Spectrometry, Mass, Electrospray Ionization
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: This internal standardization method demonstrated significantly more consistent accurate mass measurements with average mass errors ranging from -1.2 to -3.2 ppm for the 15-mer oligonucleotide used in this study. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2001 journal article

Homogeneous Preparations of 3′-Phosphoglycolate-Terminated Oligodeoxynucleotides from Bleomycin-Treated DNA as Verified by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Analytical Biochemistry, 289(2), 274–280.

By: S. Chen*, J. Hannis*, J. Flora*, D. Muddiman*, K. Charles*, Y. Yu*, L. Povirk*

MeSH headings : Alkaline Phosphatase / pharmacology; Antimetabolites, Antineoplastic / pharmacology; Bleomycin / pharmacology; Chromatography, High Pressure Liquid; DNA / drug effects; DNA Damage / drug effects; DNA Repair; Electrophoresis, Polyacrylamide Gel; Glycolates / metabolism; Mass Spectrometry; Oligonucleotides / metabolism; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared; Time Factors
topics (OpenAlex): DNA and Nucleic Acid Chemistry; Mass Spectrometry Techniques and Applications; DNA Repair Mechanisms
TL;DR: Single- and double-strand breaks bearing 3'-phosphoglycolate termini are among the most frequent lesions formed in DNA by ionizing radiation and other oxidative mutagens and could be relabeled with polynucleotide kinase to yield highly purified, high-specific-activity substrates for repair studies. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2001 journal article

Impact of ion cloud densities on the measurement of relative ion abundances in Fourier transform ion cyclotron resonance mass spectrometry: experimental observations of coulombically induced cyclotron radius perturbations and ion cloud dephasing rates

Journal of Mass Spectrometry, 36(2), 195–203.

By: E. Gordon* & D. Muddiman*

author keywords: space-charge; ion cloud density; signal decay; proteomics; quantification
MeSH headings : Cyclosporine / chemistry; Ions; Spectroscopy, Fourier Transform Infrared
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical Chemistry and Chromatography
TL;DR: Experimental evidence supports that it is the loss of cloud coherence that accounts for the signal loss over time, with the less dense cloud de-phasing more quickly, as the ion populations of the two investigated species near equivalence, so do their time constants. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2001 journal article

Perspectives on the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for short tandem repeat genotyping in the post‐genome era

Journal of Mass Spectrometry (Special Feature), 36(6), 589–606.

By: A. Null* & D. Muddiman*

author keywords: electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry; short tandem repeats; genotyping; DNA; human genome
MeSH headings : Alleles; Animals; Cyclotrons; Fourier Analysis; Genetic Markers; Genotype; Humans; Reverse Transcriptase Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Tandem Repeat Sequences / genetics
topics (OpenAlex): Mass Spectrometry Techniques and Applications; RNA and protein synthesis mechanisms; Metabolomics and Mass Spectrometry Studies
TL;DR: This perspective is intended to familiarize the mass spectrometry community with modern genomics and to report on the current state of mass Spectrometry, specifically electrospray ionization Fourier transform ion cyclotron resonance mass spectromaetry, for characterization of STRs. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 17, 2021

2001 journal article

Selective, Sensitive, and Rapid Phosphopeptide Identification in Enzymatic Digests Using ESI-FTICR-MS with Infrared Multiphoton Dissociation

Analytical Chemistry, 73(14), 3305–3311.

By: J. Flora* & D. Muddiman*

MeSH headings : Amino Acid Sequence; Animals; Caseins / analysis; Cattle; Fourier Analysis; Infrared Rays; Molecular Sequence Data; Phosphopeptides / analysis; Receptor, Insulin / analysis; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Identification and Quantification in Food
TL;DR: The sensitivity and selectivity of phosphopeptides is shown to greatly improve when the volatile base piperidine is used to adjust the pH of the digestion of naturally phosphorylated proteins bovine alpha- and beta-casein. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2000 journal article

A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in Fourier transform ion cyclotron resonance mass spectrometry

Journal of the American Society for Mass Spectrometry, 11(10), 876–883.

By: J. Hannis* & D. Muddiman*

MeSH headings : Biopolymers / chemistry; Calibration; Cyclotrons; Fourier Analysis; Mass Spectrometry; Reverse Transcriptase Polymerase Chain Reaction; Spectrophotometry, Ultraviolet
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical Chemistry and Chromatography
TL;DR: An ESI source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry, illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2000 entry reference

Absolute Quantification of Proteins by Mass Spectrometry

By: C. Shuford n & D. Muddiman n

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: All phases of the PC-IDMS workflow, from the SIL peptide production platforms to data analysis, are described and characterized in detail and an emphasis is placed on the practical quantitative aspects for each step of the workflow and their implication on quantitative accuracy. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2000 journal article

An experimental and theoretical study of the gas-phase decomposition of monoprotonated peptide nucleic acids

Journal of the American Society for Mass Spectrometry, 11(7), 615–625.

By: J. Flora*, D. Shillady* & D. Muddiman*

MeSH headings : Acetylation; Cyclotrons; Fourier Analysis; Gas Chromatography-Mass Spectrometry; Molecular Weight; Peptide Nucleic Acids / chemistry; Protons; Terminology as Topic
topics (OpenAlex): Mass Spectrometry Techniques and Applications; DNA and Nucleic Acid Chemistry; Analytical Chemistry and Chromatography
TL;DR: Electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry shows great potential as a tool for the characterization and structural elucidation of these oligonucleotides. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2000 journal article

Preparation of single-stranded PCR products for electrospray ionization mass spectrometry using the DNA repair enzyme lambda exonuclease

The Analyst, 125(4), 619–626.

By: A. Null*, J. Hannis* & D. Muddiman*

MeSH headings : Base Sequence; DNA, Single-Stranded; Exodeoxyribonucleases; Mass Spectrometry; Molecular Sequence Data; Polymerase Chain Reaction / methods; Tyrosine 3-Monooxygenase / genetics; Viral Proteins
topics (OpenAlex): Mass Spectrometry Techniques and Applications; RNA and protein synthesis mechanisms; DNA Repair Mechanisms
TL;DR: Lambda exonuclease clearly provides mass spectrometrists with a method to generate single-stranded PCR products and shows that one strand is preferentially adenylated over the other, which cannot be determined from the mass spectrum of the double-Stranded PCR product alone. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Accurate characterization of the tyrosine hydroxylase forensic allele 9.3 through development of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 13(10), 954–962.

By: J. Hannis* & D. Muddiman*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Genetic Neurodegenerative Diseases; Enzyme Structure and Function
TL;DR: The results indicate that ESI-FTICR-MS is a powerful approach to rapidly and accurately characterize tandem repeating sequences which will ultimately lead towards the understanding of a complex class of diseases and in human identity determination. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Characterization of a microdialysis approach to prepare polymerase chain reaction products for electrospray ionization mass spectrometry using on-line ultraviolet absorbance measurements and inductively coupled plasma-atomic emission spectroscopy

Rapid Communications in Mass Spectrometry, 13(5), 323–330.

By: J. Hannis* & D. Muddiman*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Ion-surface interactions and analysis
TL;DR: The desalting efficiencies for microdialysis have been quantitatively determined using inductively coupled plasma atomic emission spectroscopy (ICP-AES) by analyzing samples which mimic polymerase chain reaction (PCR) conditions and revealed an insensitivity to molecular weight over a broad range. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Consequences of Nucleic Acid Conformation on the Binding of a Trinuclear Platinum Drug†

Biochemistry, 38(45), 14731–14737.

By: M. Kloster*, J. Hannis*, D. Muddiman* & N. Farrell*

MeSH headings : Antineoplastic Agents / metabolism; DNA / metabolism; DNA, Single-Stranded / metabolism; Mass Spectrometry; Nucleic Acid Conformation; Organoplatinum Compounds / metabolism; RNA / metabolism; Spectroscopy, Fourier Transform Infrared; Structure-Activity Relationship
topics (OpenAlex): Metal complexes synthesis and properties; DNA and Nucleic Acid Chemistry; Advanced biosensing and bioanalysis techniques
TL;DR: BBR3464 binding to different nucleic acid conformations raises the possibility that the adducts of single-stranded DNA and RNA may play a role in the different antitumor efficacies of this novel drug as compared with cisplatin. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Hemoglobinase Activity of the Lysine Gingipain Protease (Kgp) of Porphyromonas gingivalis W83

Journal of Bacteriology, 181(16), 4905–4913.

By: J. Lewis*, J. Dawson*, J. Hannis*, D. Muddiman* & F. Macrina*

MeSH headings : Adhesins, Bacterial; Agar; Amino Acid Sequence; Blood; Chromatography; Chromosome Mapping; Cysteine Endopeptidases / genetics; Cysteine Endopeptidases / isolation & purification; Cysteine Endopeptidases / metabolism; Enzyme Activation / drug effects; Enzyme Inhibitors / pharmacology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Gingipain Cysteine Endopeptidases; Helminth Proteins; Hemagglutinins / genetics; Hemagglutinins / isolation & purification; Hemagglutinins / metabolism; Heme / metabolism; Hemin / pharmacology; Hemoglobins / analysis; Hemoglobins / chemistry; Hemolysis; Humans; Imidazoles / pharmacology; Iron / metabolism; Mass Spectrometry; Membrane Proteins / metabolism; Molecular Sequence Data; Mutagenesis; Peptide Fragments / analysis; Peptide Fragments / chemistry; Porphyromonas gingivalis / enzymology; Porphyromonas gingivalis / genetics; Porphyromonas gingivalis / growth & development
topics (OpenAlex): Porphyrin Metabolism and Disorders; Neonatal Health and Biochemistry; Hemoglobin structure and function
TL;DR: The ability of the Lys-gingipain to cleave human hemoglobin at Lys residues was confirmed by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of hemoglobin fragments resulting from digestion with the purified protease, and several of the predicted Hemoglobin fragments rendered by digestion with purified Lys-gesipain were detected. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Hydropathic influences on the quantification of equine heart cytochromec using relative ion abundance measurements by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Journal of Mass Spectrometry, 34(10), 1055–1062.

By: E. Gordon*, B. Mansoori*, C. Carroll* & D. Muddiman*

author keywords: proteomics; quantification; hydropathy; electrospray ionization; Fourier transform ion cyclotron resonance mass spectrometry
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Metabolomics and Mass Spectrometry Studies
TL;DR: The results present significant considerations when choosing internal standards for the quantification of large proteins by ESI-FTICR-MS and demonstrate that relative molecular ion signals in FTICR can be used to quantify macromolecular species in the nanomolar regime. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Precise mass measurement of a double-stranded 500 base-pair (309 kDa) polymerase chain reaction product by negative ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 13(12), 1201–1204.

By: D. Muddiman*, A. Null* & J. Hannis*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Protein Structure and Dynamics; RNA and protein synthesis mechanisms
TL;DR: It is demonstrated that precise mass measurements of PCR products up to 309 kDa (500 bp) can be routinely obtained by ESI-FTICR requiring low femtomole amounts. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1999 journal article

Quantification of singly charged biomolecules by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry utilizing an internal standard

Rapid Communications in Mass Spectrometry, 13(3), 164–171.

By: E. Gordon* & D. Muddiman*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

1998 journal article

Comprehensive nomenclature for the fragment ions produced from collisional activation of peptide nucleic acids

Rapid Communications in Mass Spectrometry, 12(12), 759–762.

By: J. Flora* & D. Muddiman*

topics (OpenAlex): DNA and Nucleic Acid Chemistry; Mass Spectrometry Techniques and Applications; RNA and protein synthesis mechanisms
TL;DR: It is becoming increasingly evident that multi-stage MS is especially suited to structural characterization of large bio-molecules such as PNA’s, and herein it is proposed a comprehensive nomenclature for the product ions produced upon collisional activation of PNA's, which is based upon extensive experimental studies. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1998 journal article

Heterogeneity inBacillus cereusPCR Products Detected by ESI−FTICR Mass Spectrometry

Analytical Chemistry, 70(6), 1203–1207.

By: D. Wunschel*, D. Muddiman*, K. Fox*, A. Fox* & R. Smith*

MeSH headings : Bacillus cereus / genetics; Cyclotrons; DNA, Bacterial / analysis; Fourier Analysis; Mass Spectrometry / methods; Polymerase Chain Reaction
topics (OpenAlex): Bacteriophages and microbial interactions; Bacillus and Francisella bacterial research; Genomics and Phylogenetic Studies
TL;DR: It is illustrated that PCR products amplified from templates differing by a single nucleotide can be resolved and identified using ESI-FTICR at the 89-bp level and indicated the presence of an unreported sequence variation between rRNA operons in this organism. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1998 journal article

Mass Spectrometric Characterization of DNA for Molecular Biology Applications: Advances using MALDI and ESI

Advances in Mass Spectrometry, 14, 377–406.

By: D. Wunschel, D. Muddiman & R. Smith

Source: NC State University Libraries
Added: March 17, 2021

1998 journal article

Nanoelectrospray mass spectrometry using non-metalized, tapered (50 → 10 μm) fused-silica capillaries

Rapid Communications in Mass Spectrometry, 12(8), 443–448.

By: J. Hannis* & D. Muddiman*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Analytical Chemistry and Chromatography
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1998 journal article

Sequencing and Characterization of Larger Oligonucleotides by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Reviews in Analytical Chemistry, 17(1), 1–68.

By: D. Muddiman* & R. Smith

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; RNA and protein synthesis mechanisms
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Application of sequential paired covariance to liquid chromatography-mass spectrometry data enhancements in both the signal-to-noise ratio and the resolution of analyte peaks in the chromatogram

Journal of Chromatography A, 771(1-2), 1–7.

By: D. Muddiman*, B. Huang*, G. Anderson*, A. Rockwood*, S. Hofstadler*, M. Weir-Lipton*, A. Proctor*, Q. Wu*, R. Smith*

author keywords: sequential paired covariance; chemometrics; signal-to-noise ratio; resolution
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Improving the Microdialysis Procedure for Electrospray Ionization Mass Spectrometry of Biological Samples

Journal of Mass Spectrometry, 32(4), 425–431.

By: C. Liu*, D. Muddiman*, K. Tang* & R. Smith*

author keywords: microdialysis; desalting; electrospray ionization mass spectrometry; DNA
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Microfluidic and Capillary Electrophoresis Applications; Analytical chemistry methods development
TL;DR: Significant advances in the area of microdialysis which allowed more effective handling of small volumes (microliters) of samples, more efficient desalting and enhanced mass spectrometric detection sensitivity are described. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Length and Base Composition of PCR-Amplified Nucleic Acids Using Mass Measurements from Electrospray Ionization Mass Spectrometry

Analytical Chemistry, 69(8), 1543–1549.

By: D. Muddiman*, G. Anderson*, S. Hofstadler* & R. Smith*

MeSH headings : Algorithms; Mass Spectrometry; Molecular Weight; Nucleic Acids / analysis; Nucleotides / analysis; Polymerase Chain Reaction
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies; Analytical Chemistry and Chromatography
TL;DR: It is shown that the use of modified bases in PCR or post-PCR modification chemistry allows unique solutions for the base composition of the PCR product with only modest mass measurement precision. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Instrumentation and Applications

Journal of Chemical Education, 74(11), 1288.

By: D. Muddiman*, R. Bakhtiar*, S. Hofstadler* & R. Smith*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical chemistry methods development; Analytical Chemistry and Chromatography
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Molecular Architecture via Coordination:  Marriage of Crown Ethers and Calixarenes with Molecular Squares, Unique Tetranuclear Metallamacrocycles from Metallacrown Ether and Metallacalixarene Complexes via Self-Assembly

Journal of the American Chemical Society, 119(22), 5163–5168.

By: P. Stang*, D. Cao*, K. Chen*, G. Gray*, D. Muddiman* & R. Smith*

topics (OpenAlex): Supramolecular Chemistry and Complexes; Molecular Sensors and Ion Detection; Mass Spectrometry Techniques and Applications
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Nanoscale Tectonics:  Self-Assembly, Characterization, and Chemistry of a Novel Class of Organoplatinum Square Macrocycles

Journal of the American Chemical Society, 119(48), 11611–11619.

By: J. Manna*, C. Kuehl*, J. Whiteford*, P. Stang*, D. Muddiman*, S. Hofstadler*, R. Smith*

topics (OpenAlex): Supramolecular Chemistry and Complexes; Molecular Sensors and Ion Detection; Mass Spectrometry Techniques and Applications
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1997 journal article

Transition-Metal-Mediated Rational Design and Self-Assembly of Chiral, Nanoscale Supramolecular Polyhedra with UniqueTSymmetry†

Organometallics, 16(14), 3094–3096.

By: P. Stang, B. Olenyuk*, D. Muddiman* & R. Smith*

topics (OpenAlex): Supramolecular Chemistry and Complexes; Supramolecular Self-Assembly in Materials; Surface Chemistry and Catalysis
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1996 journal article

A Quantitative Study ofin vitroHepatic Metabolism of Tacrolimus (FK506) Using Secondary Ion and Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Rapid Communications in Mass Spectrometry, 10(10), 1215–1218.

By: A. Gusev*, D. Muddiman*, A. Proctor*, A. Sharkey*, P. Tata*, R. Venkataramanan*, D. Hercules*

topics (OpenAlex): Pharmacological Effects and Toxicity Studies; Renal Transplantation Outcomes and Treatments; HIV/AIDS drug development and treatment
TL;DR: The identification and simultaneous quantification of Tacrolimus and its hepatic metabolites in baboons has been achieved using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry and static secondary-ion mass spectromaetry and TOF-SIMS. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1996 journal article

Analysis of Double-stranded Polymerase Chain Reaction Products from the Bacillus cereus Group by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Rapid Communications in Mass Spectrometry, 10(1), 29–35.

By: D. Wunschel, K. Fox, A. Fox, J. Bruce, D. Muddiman & R. Smith

Source: NC State University Libraries
Added: March 8, 2021

1996 journal article

Characterization of PCR Products from Bacilli Using Electrospray Ionization FTICR Mass Spectrometry

Analytical Chemistry, 68(21), 3705–3712.

By: D. Muddiman*, D. Wunschel*, C. Liu*, L. Paša-Tolić*, K. Fox*, A. Fox*, G. Anderson*, R. Smith*

MeSH headings : Bacillus / genetics; Mass Spectrometry; Polymerase Chain Reaction; Spectroscopy, Fourier Transform Infrared
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Advanced Proteomics Techniques and Applications; Enzyme Structure and Function
TL;DR: This constitutes the first report demonstrating the ionization and detection of PCR products by mass spectrometry with mass precision and accuracy for assignment of such modifications or substitutions. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

1996 journal article

Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry

Journal of the American Society for Mass Spectrometry, 7(8), 697–706.

By: D. Muddiman*, X. Cheng*, H. Udseth* & R. Smith*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
TL;DR: Several oligonucleotides that varied in base composition and length were investigated, and studies of mixtures showed a significant reduction in spectral complexity. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1996 journal article

Design and Self-Assembly of Nanoscale Organoplatinum Macrocycles

Journal of the American Chemical Society, 118(36), 8731–8732.

By: J. Manna*, J. Whiteford*, P. Stang, D. Muddiman* & R. Smith*

topics (OpenAlex): Supramolecular Chemistry and Complexes; Molecular Sensors and Ion Detection; Surface Chemistry and Catalysis
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1996 journal article

Direct quantification of cocaine in urine by time-of-flight mass spectrometry

Fresenius' Journal of Analytical Chemistry, 354(1), 103–110.

By: D. Muddiman*, A. Gusev*, L. Martin* & D. Hercules*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Forensic Toxicology and Drug Analysis; Analytical chemistry methods development
TL;DR: The direct quantification of cocaine in urine has been achieved using time-of-flight secondary-ion mass spectrometry (TOF-SIMS) using a dynamic range of four orders of magnitude using one internal standard concentration. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1996 journal article

Enhancement of ion intensity in time-of-flight secondary-ionization mass spectrometry

Journal of the American Society for Mass Spectrometry, 7(5), 467–472.

By: A. Nicola*, D. Muddiman* & D. Hercules*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Forensic Toxicology and Drug Analysis
TL;DR: A sample preparation technique is discovered that enhanced the SI yield of cyclosporin A (CsA) in an allograft patient sample and neat samples of CsA and polystyrene when deposited from neat solutions. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1996 journal article

Important Aspects concerning the Quantification of Biomolecules by Time-of-Flight Secondary-Ion Mass Spectrometry

Applied Spectroscopy, 50(2), 161–166.

By: D. Muddiman*, A. Nicola*, A. Proctor* & D. Hercules*

author keywords: SIMS; quantification; mass spectrometry; TOF-SIMS
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical chemistry methods development
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1995 journal article

Application of Sequential Paired Covariance to Capillary Electrophoresis Electrospray Ionization Time-of-Flight Mass Spectrometry: Unraveling the Signal from the Noise in the Electropherogram

Analytical Chemistry, 67(23), 4371–4375.

By: D. Muddiman*, A. Rockwood, Q. Gao, J. Severs, H. Udseth, R. Smith, A. Proctor

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Analytical Chemistry and Chromatography; Advanced Chemical Sensor Technologies
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1995 journal article

Application of secondary ion and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the quantitative analysis of biological molecules

Mass Spectrometry Reviews, 14(6), 383–429.

By: D. Muddiman*, A. Gusev* & D. Hercules*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical Chemistry and Chromatography
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1995 journal article

Simultaneous quantification of cyclosporin A and its major metabolites by time-of-flight secondary-ion mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry utilizing data analysis techniques: Comparison with high-performance liquid chromatography

Journal of Mass Spectrometry, 30(10), 1469–1479.

By: D. Muddiman*, A. Gusev*, K. Stoppek-Langner*, A. Proctor*, D. Hercules*, P. Tata*, R. Venkataramanan*, W. Diven*

topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical chemistry methods development
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

1994 journal article

Characterization of Polystyrene on Etched Silver Using Ion Scattering and X-ray Photoelectron Spectroscopy: Correlation of Secondary Ion Yield in Time-of-Flight SIMS with Surface Coverage

The Journal of Physical Chemistry, 98(44), 11570–11575.

By: D. Muddiman*, A. Brockman, A. Proctor, M. Houalla & D. Hercules

topics (OpenAlex): Ion-surface interactions and analysis; Analytical chemistry methods development; Mass Spectrometry Techniques and Applications
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

1994 journal article

Quantitative Measurement of Cyclosporin A in Blood by Time-of-Flight Mass Spectrometry

Analytical Chemistry, 66(14), 2362–2368.

By: D. Muddiman*, A. Gusev, A. Proctor, D. Hercules, R. Venkataraman & W. Diven

MeSH headings : Chromatography, High Pressure Liquid; Cyclosporine / blood; Fluorescence Polarization Immunoassay; Humans; Mass Spectrometry; Spectrometry, Mass, Fast Atom Bombardment
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Ion-surface interactions and analysis; Analytical chemistry methods development
TL;DR: Blood samples were analyzed by a nonspecific method, fluorescence polarization immunoassay (FPIA), and by a specific method, high-performance liquid chromatography (HPLC), and the TOF-MS results are in good agreement with the HPLC results, but not with the FPIA results. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

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Updated: September 23rd, 2025 04:16

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