Works (378)
Updated: April 22nd, 2024 07:45
2024 journal article
A statistical approach to system suitability testing for mass spectrometry imaging
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 38(9).
onlinelibrary.wiley.com (publisher PDF)
Sources: Web Of Science, ORCID
Added: March 18, 2024
2024 journal article
Prototyping an ionization source for non-engineers
JOURNAL OF MASS SPECTROMETRY, 59(1).
author keywords: computer-aided design; hardware interfacing; instrumentation prototyping; microcontroller; software development
Sources: Web Of Science, ORCID
Added: January 2, 2024
2024 journal article
Quantitative mass spectrometry imaging (qMSI): A tutorial
JOURNAL OF MASS SPECTROMETRY, 59(4).
author keywords: absolute quantification; mass spectrometry imaging; qMSI; relative quantification
TL;DR:
This tutorial will detail the main factors to consider when designing a qMSI experiment and highlight the methods that have been developed to overcome these added complexities, specifically for those newer to the field of MSI.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: March 25, 2024
2024 article
RCM protocols: improving reproducibility in the field of mass spectrometry
Holman, S. W., Langley, G. J., Muddiman, D. C., & Novak-Mitchell, C. (2024, January 2). RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Vol. 1.
Sources: Web Of Science, ORCID
Added: January 16, 2024
2023 journal article
Achieving Cross-Ring Fragmentation of <i>N</i>-Linked Glycans by IR-MALDESI
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 35(1), 166–171.
author keywords: N-linked glycans; lithium doping; fragmentation; IR-MALDESI; cross-ring fragment
Sources: Web Of Science, ORCID
Added: January 16, 2024
2023 article
Achieving Sub-Parts-per-Million Mass Measurement Accuracy on an Orbitrap Mass Spectrometry Imaging Platform without Automatic Gain Control
Kibbe, R. R., & Muddiman, D. C. (2023, April 25). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 4.
author keywords: centroid; mass measurement accuracy; IR-MALDESI; mass spectrometry imaging; Orbitrap; automatic gain control
MeSH headings : Animals; Mice; Mass Spectrometry / methods; Data Accuracy; Lipids
TL;DR:
There is support that natively collecting centroid MS data significantly improves MMA to sub-ppm levels without AGC and reduces false positive annotations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: May 15, 2023
2023 article
Automatic z-Axis Correction for IR-MALDESI Mass Spectrometry Imaging of Uneven Surfaces
Xi, Y., Knizner, K. T. T., Garrard, K. P. P., & Muddiman, D. C. C. (2023, June 12). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 6.
author keywords: mass spectrometry imaging; nonflat surfaces; IR-MALDESI; automatic z-axis correction
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: June 26, 2023
2023 article
Comparative analysis of sucrose-embedding for whole-body zebrafish MSI by IR-MALDESI
Wang, M. F., Ritter, M. M., Kullman, S. W., & Muddiman, D. C. (2023, August 29). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 8.
author keywords: IR-MALDESI; Mass spectrometry imaging; Sucrose; PFA; Zebrafish
TL;DR:
Danio rerio, or zebrafish, are model organisms for various disease states and can be imaged as a multi-organ sample to analyze morphological and metabolomic preservation as a result of sample preparation to compare sucrose-embedding with paraformaldehyde fixation against conventional fresh-frozen sample preparation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: September 25, 2023
2023 article
Development of an object-based image analysis tool for mass spectrometry imaging ion classification
Eisenberg, S. M., Knizner, K. T., & Muddiman, D. C. (2023, May 24). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 5.
author keywords: IR-MALDESI; Object-based image analysis; Mass spectrometry imaging; Image segmentation; Image classification; MATLAB
TL;DR:
The utility of an ion classification tool (ICT) developed using object-based image analysis in MATLAB is presented, which was able to accurately classify 45/50 ions as on-tissue or background.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: July 3, 2023
2023 journal article
Enhanced Detection of Charged N-Glycans in the Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometric Imaging
ANALYTICAL CHEMISTRY, 95(29), 10913–10920.
TL;DR:
This work demonstrates the first application of IR-MALDESI in N-linked glycan imaging of the brain tissue, leading to a 2.5-fold increase in the in situ total brain N-glycan detection compared to the current gold standard of positive-mode matrix-assisted laser desorption/ionization mass spectrometry imaging.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: July 31, 2023
2023 journal article
Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 37(22).
TL;DR:
This work defines an optimal sample thickness for IR-MALDESI-MSI and demonstrates the utility of optimizing tissue thickness for MSI platforms of comparable Z resolution.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: October 30, 2023
2023 article
Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non-flat samples
Joignant, A. N. N., Xi, Y., & Muddiman, D. C. C. (2023, March 14). JOURNAL OF MASS SPECTROMETRY, Vol. 3.
author keywords: depth of focus; laser wavelength; mass spectrometry imaging; spatial resolution; surface roughness
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: April 4, 2023
2023 article
Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI
Wang, M. F., Sohn, A. L., Samal, J., Erning, K., Segura, T., & Muddiman, D. C. (2023, March 29). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 3.
author keywords: IR-MALDESI; mass spectrometry imaging; sucrose; PFA
MeSH headings : Mice; Animals; Spectrometry, Mass, Electrospray Ionization / methods; Lipidomics; Ice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ions / chemistry; Lipids / analysis; Brain
TL;DR:
Evidence from these investigations supports sucrose-embedding without ice matrix as an alternative preparatory technique for IR-MALDESI-MSI, and demonstrates improved spatial distribution of ions resulting from the cryo-protective property of sucrose and paraformaldehyde fixation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: April 24, 2023
2023 article
Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs)
Joignant, A. N., Ritter, M. M., Knizner, K. T., Garrard, K. P., Kullman, S. W., & Muddiman, D. C. (2023, August 1). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 8.
author keywords: IR-MALDESI; mass spectrometry imaging; spatialresolution; nested region of interest; zebrafish
TL;DR:
The nROI method was characterized on mouse liver and applied to top-hat MSI of zebrafish using a novel optical train, which resulted in a significant improvement in both acquisition time and spatial detail of the zebra fish.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 21, 2023
2023 article
Metabolite Annotation Confidence Score (MACS): A Novel MSI Identification Scoring Tool
Eisenberg, S. M., Knizner, K. T., & Muddiman, D. C. (2023, August 22). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 8.
author keywords: IR-MALDESI; annotation scoring; mass measurementaccuracy; spectral accuracy; SSIM; MATLAB
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: September 5, 2023
2023 article
On the importance of color in mass spectrometry imaging (vol 57, e4898, 2022)
Muddiman, D. C. (2023, May). JOURNAL OF MASS SPECTROMETRY, Vol. 58.
Sources: Web Of Science, ORCID
Added: May 15, 2023
2023 article
Predicting Sialic Acid Content of N-Linked Glycans Using the Isotopic Pattern of Chlorine
Palomino, T. V., & Muddiman, D. C. (2023, June 8). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 6.
author keywords: N-linked glycans; sialic acid; chlorine; IR-MALDESI
MeSH headings : Humans; N-Acetylneuraminic Acid; Chlorine; Polysaccharides / chemistry; Sialic Acids / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A new rule is described that can predict the number of sialic acids on a glycan and grants confident glycan annotations and compositions beyond accurate mass measurements, thereby further improving the capability of IR-MALDESI to study sialylated N-linked glycans within biological tissues.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: June 26, 2023
2023 journal article
Quasi-continuous infrared matrix-assisted laser desorption electrospray ionization source coupled to a quadrupole time-of-flight mass spectrometer for direct analysis from well plates
JOURNAL OF MASS SPECTROMETRY, 58(1).
author keywords: ambient ionization; direct analysis; IR-MALDESI; Q-TOF mass spectrometer
TL;DR:
An infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) source coupled directly to an Agilent 6545 for direct analysis from well plates and is a proof of concept for HTS applications using IR‐MAL DESI coupled to a different MS platform.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: February 13, 2023
2023 journal article
SMART: A data reporting standard for mass spectrometry imaging
JOURNAL OF MASS SPECTROMETRY, 58(2).
author keywords: data reporting standards; mass spectrometry imaging
MeSH headings : Research Design; Mass Spectrometry / methods; Ions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
The goal of this tutorial is to discuss the necessity of establishing a uniform reporting system to communicate MSI data in publications and presentations in a simple format to readily interpret the parameters and baseline outcomes of the data.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: March 13, 2023
2023 article
Time of acquisition and high spatial resolution mass spectrometry imaging (vol 58, e4911, 2023)
Muddiman, D. C. (2023, May). JOURNAL OF MASS SPECTROMETRY, Vol. 58.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: May 15, 2023
2023 journal article
Transforming a Mid-infrared Laser Profile from Gaussian to a Top- Hat with a Diffractive Optical Element for Mass Spectrometry Imaging
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 34(1), 10–16.
author keywords: diffractive optical element; top-hat; infrared; IR-MALDESI; 3D MSI
MeSH headings : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Electrospray Ionization / methods; Lasers; Light
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: January 30, 2023
2022 journal article
An adaptive teosinte mexicana introgression modulates phosphatidylcholine levels and is associated with maize flowering time
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(27).
Contributors: P. Thompson n
author keywords: phospholipid metabolism; maize genetics; highland adaptation; flowering time; selection
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
15. Life on Land
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 3, 2022
2022 journal article
Developing transmission mode for infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 36(22).
MeSH headings : Animals; Glycerol; Ice; Lasers; Mammals; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
This work has successfully demonstrated the adaptation of the NextGen IR‐MAL DESI source through the feasibility of tm‐IR‐MALDESI mass spectrometry imaging on mammalian tissue, expanding future biological applications of the method.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: September 26, 2022
2022 journal article
Development and validation of a high resolving power absolute quantitative per- and polyfluoroalkyl substances method incorporating Skyline data processing
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 36(11).
MeSH headings : Chromatography, Liquid; Fluorocarbons; Mass Spectrometry / methods; Reproducibility of Results; Software
TL;DR:
This method demonstrates the feasibility of performing reproducible absolute quantitation of PFAS on an HRAM platform and does so using an open-source vendor-neutral data processing software to facilitate sharing of data across labs and institutions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, ORCID
Added: April 4, 2022
2022 journal article
Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance
CELL METABOLISM, 34(7), 1042-+.
MeSH headings : Blood Glucose; Diabetes Mellitus, Type 2; Diet, High-Fat / adverse effects; Dietary Fats / pharmacology; Fructose / metabolism; Glucose / metabolism; Glucose Intolerance / metabolism; Humans; Insulin / metabolism; Islets of Langerhans / metabolism
TL;DR:
It is reported that dietary fat enhances intestinal fructose metabolism, which releases glycerate into the blood, which provides a link between dietary fructose and diabetes that is modulated by dietary fat.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 8, 2022
2022 article
Highlighting Functional Mass Spectrometry Imaging Methods in Bioanalysis
Mellinger, A. L., Muddiman, D. C., & Gamcsik, M. P. (2022, June 27). JOURNAL OF PROTEOME RESEARCH, Vol. 6.
author keywords: mass spectrometry imaging; kinetics; rate; isotope; metabolism; dynamics
MeSH headings : Magnetic Resonance Imaging; Mass Spectrometry / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
MSI exceeds some of the classic functional imaging methods, demonstrating the ability to provide functional data from the nanoscale (subcellular) to whole tissue or organ level, and this Perspective highlights several examples of how different MSI ionization and detection technologies can provide unprecedented detailed spatial maps of time-dependent biological processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: July 11, 2022
2022 journal article
Improved spatial resolution of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 36(23).
MeSH headings : Animals; Spectrometry, Mass, Electrospray Ionization / methods; Ice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers; Mammals
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: October 17, 2022
2022 journal article
Interrogating the Metabolomic Profile of Amyotrophic Lateral Sclerosis in the Post-Mortem Human Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging (MSI)
METABOLITES, 12(11).
author keywords: IR-MALDESI MSI; mass spectrometry imaging; Amyotrophic lateral sclerosis (ALS); neurodegenerative disease; multiomic
TL;DR:
This work is the first of its kind, reporting on the investigation of ALS post-mortem human brain tissue analyzed by multiomic MSI, and nearly 300 metabolites were identified across the sixteen samples where 25 were identified as dysregulated between disease cohorts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: January 3, 2023
2022 journal article
Mapping glycine uptake and its metabolic conversion to glutathione in mouse mammary tumors using functional mass spectrometry imaging
FREE RADICAL BIOLOGY AND MEDICINE, 193, 677–684.
MeSH headings : Mice; Animals; Glycine; Biological Transport; Glutathione; Mammary Neoplasms, Animal; Mass Spectrometry
TL;DR:
A novel functional mass spectrometry imaging (fMSI) method that can map the variations in the conversion of glycine to glutathione metabolic activity across tumor tissue sections by tracking the fate of three glycine isotopologues administered in a timed sequence to tumor-bearing anesthetized mice is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: January 23, 2023
2022 journal article
Mass Spectrometry Imaging of N-Linked Glycans in a Formalin-Fixed Paraffin-Embedded Human Prostate by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization
JOURNAL OF PROTEOME RESEARCH, 21(1), 243–249.
author keywords: IR-MALDESI; N-linked glycans; mass spectrometry imaging; prostate cancer
MeSH headings : Formaldehyde / chemistry; Humans; Lasers; Male; Paraffin Embedding; Polysaccharides / chemistry; Prostate / chemistry; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
This work demonstrates the first steps in N-linked glycan imaging of biological tissues by IR-MALDESI MSI, a hybrid ionization technique commonly used for mass spectrometry imaging (MSI) applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: February 7, 2022
2022 journal article
Multimodal Mass Spectrometry Imaging of Rat Brain Using IR-MALDESI and NanoPOTS-LC-MS/MS
JOURNAL OF PROTEOME RESEARCH, 21(3), 713–720.
author keywords: multimodal mass spectrometry imaging; multiomic analyses; IR-MALDESI; nanoPOTS-LC-MS/MS
MeSH headings : Animals; Brain / diagnostic imaging; Chromatography, Liquid / methods; Proteome; Proteomics; Rats; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry
TL;DR:
A multiomic pathway integration showed a strong correlation between the two data sets when comparing average abundances of metabolites and corresponding enzymes in each brain region, and the first steps in the creation of a multimodal MSI technique that combines two highly sensitive and complementary imaging platforms are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: May 2, 2022
2022 journal article
Multiple Infusion Start Time Mass Spectrometry Imaging of Dynamic SIL-Glutathione Biosynthesis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization
JOURNAL OF PROTEOME RESEARCH, 21(3), 747–757.
author keywords: IR-MALDESI; mass spectrometry imaging; stable isotope labeling; glutathione; kinetic metabolism
MeSH headings : Animals; Glutathione; Glycine; Lasers; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A study combining a multiple infusion start time protocol, stable isotope labeling technology, infrared matrix-assisted laser desorption electrospray ionization, and high-resolution accurate mass-mass spectrometry imaging to study spatial changes in glutathione kinetics across in sectioned mouse liver tissues is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: May 2, 2022
2022 article
Next-Generation Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Source for Mass Spectrometry Imaging and High-Throughput Screening
Knizner, K. T., Guymon, J. P., Garrard, K. P., Bouvree, G., Manni, J., Hauschild, J.-P., … Muddiman, D. C. (2022, September 29). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 9.
author keywords: IR-MALDESI; High-Throughput Screening; Mass Spectrometry Imaging; Orbitrap Mass Spectrometer; Design of Experiments
MeSH headings : High-Throughput Screening Assays; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: October 17, 2022
2022 journal article
Normalization techniques for high-throughput screening by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry
JOURNAL OF MASS SPECTROMETRY, 57(6).
author keywords: high-throughput screening; IR-MALDESI; normalization; Orbitrap mass spectrometer
MeSH headings : Drug Discovery; High-Throughput Screening Assays; Lasers; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Optimal solvent compositions for increasing measured analyte abundances with direct analysis by IR‐MALDESI‐MS are reported and normalization strategies, such as adding a normalization standard that is similar or dissimilar in structure to the model target drug, are evaluated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: June 20, 2022
2022 journal article
Novel matrix strategies for improved ionization and spatial resolution using IR-MALDESI mass spectrometry imaging
JOURNAL OF MASS SPECTROMETRY, 57(8).
author keywords: energy-absorbing matrix; glycerol; IR-MALDESI; mass spectrometry imaging
MeSH headings : Animals; Glycerol; Ice; Lipids; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
It was observed that utilizing a 50% glycerol matrix without ice provides improved spatial resolution with lipid abundances and annotations comparable to the ice matrix standard, while decreasing the time required to complete an IR‐MALDESI tissue imaging experiment.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 8, 2022
2022 journal article
On the importance of color in mass spectrometry imaging
JOURNAL OF MASS SPECTROMETRY, 57(12).
author keywords: colormap; CVD; data visualization; MSI
MeSH headings : Humans; Mass Spectrometry
TL;DR:
Best practices for choosing a colormap to accurately display MSI data, improve readability, and accommodate all CVDs are presented.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: January 9, 2023
2022 journal article
Optimized C-Trap Timing of an Orbitrap 240 Mass Spectrometer for High-Throughput Screening and Native MS by IR-MALDESI
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 33(2), 328–334.
author keywords: high-throughput screening; IR-MALDESI; Orbitrap mass spectrometer; C-trap
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: March 7, 2022
2022 article
Phosphorylation-dependent proteome of Marcks in ependyma during aging and behavioral homeostasis in the mouse forebrain
Muthusamy, N., Williams, T. I., O'Toole, R., Brudvig, J. J., Adler, K. B., Weimer, J. M., … Ghashghaei, H. T. (2022, January 24). GEROSCIENCE, Vol. 1.
Contributors: N. Muthusamy n, T. Williams n , R. O’Toole*, J. Brudvig *, K. Adler n, J. Weimer *, n , H. Ghashghaei n
author keywords: Aging; Behavior; Clca3; Ependymal cells; Forebrain; Marcks; Mass spectrometry; Neuroinflammation
MeSH headings : Mice; Animals; Phosphorylation; Ependyma / metabolism; Intracellular Signaling Peptides and Proteins / metabolism; Myristoylated Alanine-Rich C Kinase Substrate / metabolism; Proteome / metabolism; Membrane Proteins / metabolism; Protein Kinase C / metabolism; Homeostasis; Prosencephalon / metabolism; Mammals / metabolism
TL;DR:
This study provides first line of evidence for potential mechanisms that may mediate differential Marcks functions in young and old ECs, and their effect on forebrain homeostasis during aging.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: January 31, 2022
2021 journal article
Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization
METABOLITES, 11(12).
author keywords: metabolites; dicationic reagent; ambient ionization; IR-MALDESI; mass spectrometry imaging
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: January 10, 2022
2021 journal article
Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa
FRONTIERS IN PLANT SCIENCE, 12.
author keywords: protein interaction; monolignol biosynthesis; wood formation; Populus trichocarpa; BiFC; metabolic flux
TL;DR:
It is proposed that Ptr4CL/PtrHCT complexes modulate the metabolic flux of CoA ligation for monolignol biosynthesis during wood formation in P. trichocarpa.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: November 8, 2021
2021 journal article
GlycoHunter: An Open-Source Software for the Detection and Relative Quantification of INLIGHT-Labeled N-Linked Glycans
JOURNAL OF PROTEOME RESEARCH, 20(4), 1855–1863.
author keywords: glycomics; INLIGHT; N-linked glycans; data analysis; open source; software
TL;DR:
GlycoHunter, a user-friendly software created in MATLAB that enables researchers to accurately and efficiently process MS1 glycomics data where a NAT and SIL pair is generated for relative quantification, including but not limited to, INLIGHT.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: May 3, 2021
2021 journal article
In situ detection of fatty acid C=C positional isomers by coupling on-tissue mCPBA epoxidation with infrared matrix-assisted laser desorption electrospray ionization mass spectrometry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 35(13).
TL;DR:
The on-tissue mCPBA epoxidation was implemented into an ambient MS imaging workflow to offer a rapid and simple way for in situ identification and relative quantification of double bond positional isomers without the requirement for instrument modification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: July 6, 2021
2021 journal article
Investigations of beta-carotene radical cation formation in infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 35(16).
MeSH headings : Cations / analysis; Cations / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry; beta Carotene / analysis; beta Carotene / chemistry
TL;DR:
There are several potential mechanisms for β-carotene radical cationization prior to IR-MALDESI analysis with multiphoton-ionization, thermal degradation, and/or reaction with oxygen, but changing certain aspects of sample conditions can result in significant mixed-mode ionization with competing protonation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: July 26, 2021
2021 article
Obituary Michael S. Bereman (1981-2021)
Muddiman, D. C., & MacCoss, M. J. (2021, May 5). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 32, pp. 1272–1274.
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, ORCID
Added: June 10, 2021
2021 journal article
Spatially resolved metabolomic characterization of muscle invasive bladder cancer by mass spectrometry imaging
METABOLOMICS, 17(8).
author keywords: Bladder cancer; IR-MALDESI mass spectrometry imaging; Metabolomics; Lipidomics; Intratumor heterogeneity
MeSH headings : Feasibility Studies; Humans; Metabolomics / methods; Muscle, Smooth / metabolism; Muscle, Smooth / pathology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Urinary Bladder Neoplasms / diagnostic imaging; Urinary Bladder Neoplasms / metabolism; Urinary Bladder Neoplasms / pathology
TL;DR:
Great promise is demonstrated of IR-MALDESI MSI to identify molecular changes derived from cancer and unveil tumor heterogeneity, which can potentially promote the discovery of clinically relevant biomarkers and allow for applications in precision medicine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 2, 2021
2021 article
The development and application of matrix assisted laser desorption electrospray ionization: The teenage years
Bagley, M. C., Garrard, K. P., & Muddiman, D. C. (2021, May 24). MASS SPECTROMETRY REVIEWS, Vol. 5.
author keywords: ambient ionization; direct analysis; FTMS; IR lasers; MALDESI; mass spectrometry imaging; MSiReader
MeSH headings : Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Lasers
TL;DR:
This review will discuss the developments of MALDESI since its first laboratory demonstration in 2005 to the most recent advances in 2021.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: June 10, 2021
2021 journal article
Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 413(10), 2793–2801.
author keywords: 3D; Mass spectrometry imaging; IR-MALDESI; Skin; Lipids
MeSH headings : Animals; Imaging, Three-Dimensional / methods; Lipids / analysis; Mice; Mice, Hairless; Skin / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
The use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: January 25, 2021
2021 article
Understanding the electrospray ionization response factors of per- and poly-fluoroalkyl substances (PFAS)
Enders, J. R., Grace M. O'Neill, Whitten, J. L., & Muddiman, D. C. (2021, July 21). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 7.
author keywords: Electrospray ionization; PFAS; logP; Surface activity
TL;DR:
A method for predicting the behavior of individual PFAS compounds, including relative retention order in chromatography, is presented which is simply based on the number of fluorine atoms in the molecule as well as the class of the compound.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 2, 2021
2021 article
Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT (TM) derivatized N-linked glycans in biological samples
Butler, K. E., Kalmar, J. G., Muddiman, D. C., & Baker, E. S. (2021, August 4). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 8.
author keywords: Glycomics; Ion mobility spectrometry; INLIGHT (TM); N-Linked glycans; LC-IMS-MS
TL;DR:
The combination of IMS and the natural (NAT) and stable-isotope label (SIL) in the INLIGHT™ strategy provided additional confidence for each glycan identification due to the mobility aligned NAT- and SIL-labeled glycans and further capabilities for isomer examinations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 16, 2021
2020 journal article
3D Imaging and metabolomic profiling reveal higher neuroactive kavalactone contents in lateral roots and crown root peels of Piper methysticum (kava)
GIGASCIENCE, 9(9).
author keywords: kava; kavalactones; metabolomics; 3D imaging; mass spectrometry imaging
MeSH headings : Imaging, Three-Dimensional; Kava; Lactones; Metabolomics; X-Ray Microtomography
TL;DR:
Detailed metabolomic profile and spatio-temporal characteristics of tissues from the roots and stems are characterized using cross-platform metabolomics and a 3D imaging approach to provide mechanistic insights into the social and clinical practice of the use of only peeled roots.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: November 16, 2020
2020 journal article
A Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns
Journal of the American Society for Mass Spectrometry, 31(12), 2547–2552.
author keywords: Mass Spectrometry Imaging; Arbitrary Region of Interest; MALDESI; Vision-System
TL;DR:
A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns that reduces contamination from off-sample material and significantly reduces acquisition time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: January 19, 2021
2020 journal article
Analysis of neurotransmitters in rat placenta exposed to flame retardants using IR-MALDESI mass spectrometry imaging
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(15), 3745–3752.
author keywords: IR-MALDESI; Mass spectrometry imaging; Neurotransmitters; Exposomics
MeSH headings : Animals; Female; Flame Retardants / adverse effects; Infrared Rays; Neurotransmitter Agents / analysis; Optical Imaging / instrumentation; Optical Imaging / methods; Placenta / chemistry; Placenta / diagnostic imaging; Pregnancy; Rats; Rats, Wistar; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Changes in the abundance and spatial distribution of neurotransmitters in rat placenta following chemical exposure could be determined using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: June 1, 2020
2020 journal article
Comparative Proteomic Analysis of Wild Type and Mutant Lacking an SCF E3 Ligase F-Box Protein in Magnaporthe oryzae
JOURNAL OF PROTEOME RESEARCH, 19(9), 3761–3768.
MeSH headings : Ascomycota; F-Box Proteins / genetics; Fungal Proteins / genetics; Magnaporthe / metabolism; Proteomics; Ubiquitin-Protein Ligases / genetics
TL;DR:
A label-free quantitative global proteomics technique was used to probe the role of ubiquitination and phosphorylation in the mechanism of how E3 ligase regulates change in virulence of M. oryzae and greatly assist in the selection of future genes for functional studies and enabling mechanistic insight related to virulence.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: October 5, 2020
2020 journal article
Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications
Journal of the American Society for Mass Spectrometry, 31(3), 642–650.
author keywords: mass spectrometry imaging; ion mobility spectrometry; IR-MALDESI; high-throughput; isomer resolution
TL;DR:
To increase a range of detectable features in IR-MALDESI experiments, the home-built ion source was integrated with a commercially available drift tube ion mobility spec-trometer-mass spectrometer (IMS-MS) and analyzed small polar molecules, lipids, carbohydrates as well as intact proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, Web Of Science
Added: March 23, 2020
2020 journal article
Determination of Optimal Electrospray Parameters for Lipidomics in Infrared-Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 31(2), 319–325.
author keywords: IR-MALDESI; mass spectrometry imaging: electrospray ionization; metabolites; lipids
MeSH headings : Animals; Lipidomics / methods; Lipids / analysis; Liver / chemistry; Liver / diagnostic imaging; Molecular Imaging / methods; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Using these defined parameters, IR-MALDESI positive polarity lipidomics studies can increase lipid abundances 3-fold, with 15% greater coverage, while an abundance increase of 1.5-fold and 10% more coverage can be achieved relative to commonly used parameters in negative polarity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: March 10, 2020
2020 journal article
Development of a relative quantification method for infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging of Arabidopsis seedlings
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 34(6).
MeSH headings : Arabidopsis / chemistry; Arabidopsis / genetics; Arabidopsis Proteins / genetics; Indoleacetic Acids / analysis; Indoles / analysis; Mutation; Oximes / analysis; Oxygenases / genetics; Seedlings / chemistry; Seedlings / genetics; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
IAA doping of agarose was demonstrated as a viable technique for relative quantification of metabolites in live seedlings or tissues with similar biological considerations and was shown to be an appropriate sampling surface for IR-MALDESI mass spectrometry imaging of Arabidopsis seedlings.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: March 10, 2020
2020 journal article
Direct Analysis of Native N-Linked Glycans by IR-MALDESI
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 31(8), 1759–1762.
author keywords: IR-MALDESI; N-linked glycans; glycomics; direct analysis
TL;DR:
This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 31, 2020
2020 journal article
Effects of Prenatal Exposure to a Mixture of Organophosphhate Flame Ritardants on Placental Gene Expression and Serotonergic Innervaion in the Fetal Rat Brain
TOXICOLOGICAL SCIENCES, 176(1), 203–223.
author keywords: endocrine disruptors; endocrine toxicology; flame retardants; metabolome; neurotoxicity; developmental; neurotoxicology; neurotransmitter; developmental toxicity; prenatal; reproductive and developmental toxicology; developmental/teratology
MeSH headings : Animals; Brain / drug effects; Female; Fetal Development; Fetus; Flame Retardants / toxicity; Gene Expression; Humans; Male; Organophosphates; Organophosphorus Compounds; Placenta / metabolism; Plasticizers; Polybrominated Biphenyls; Pregnancy; Prenatal Exposure Delayed Effects; Rats; Rats, Wistar; Serotonin / metabolism
TL;DR:
Findings suggest that OPFRs have the potential to impact the 5-HTergic system in the fetal forebrain by disrupting placental Trp metabolism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Web Of Science, ORCID
Added: December 14, 2020
2020 journal article
Enhanced protocol for quantitativeN-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)(TM)
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(27), 7569–7579.
author keywords: Glycomics; Orbitrap; Sample preparation; INLIGHT (TM); N-linked glycans; HILIC; PGC; Reversed-phase; Chromatography
MeSH headings : Chromatography, Reverse-Phase / methods; Female; Fetuins / chemistry; Glycomics / methods; Glycosylation; Humans; Male; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / chemistry; Polysaccharides / analysis; Tandem Mass Spectrometry / methods
TL;DR:
To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated and the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)3, was possible.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: September 14, 2020
2020 journal article
Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging analysis of endogenous metabolites in cherry tomatoes
ANALYST, 145(16), 5516–5523.
MeSH headings : Lasers; Lipids; Solanum lycopersicum; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR:
The spatially resolved metabolic profiling of cherry tomatoes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI), a mass spectrometry imaging technique that operates at ambient conditions and requires no sample derivatization, finds endogenous carotenoid hydrocarbons ionized as radical cations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 31, 2020
2020 journal article
Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(1), 139–147.
author keywords: IR-MALDESI; Mass spectrometry imaging; Orbitrap; Magnaporthe oryzae; Image recognition; Polarity switching
MeSH headings : Hordeum / metabolism; Hordeum / microbiology; Host-Pathogen Interactions; Infrared Rays; Magnaporthe / metabolism; Magnaporthe / physiology; Metabolomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Serotonin, a barley defense metabolite, was a compound identified in both positive and negative modes, and metabolites in the melanin pathway, important for infection development of M. oryzae were identified but were unable to be confirmed with MS/MS due to their low abundances.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: December 9, 2019
2020 journal article
Lipidomic profiling of single mammalian cells by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(29), 8211–8222.
author keywords: Single-cell analysis; Lipidome; Mass spectrometry; IR-MALDESI; Orbitrap
MeSH headings : Animals; HeLa Cells; Humans; Limit of Detection; Lipidomics; Rats; Reproducibility of Results; Single-Cell Analysis / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A rapid and sensitive method for investigating the lipid profiles of isolated single cells using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry (IR-MALDESI-MS), which provides an important basis for future work on differentiation between normal and diseased cells at various developmental states.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: October 12, 2020
2020 journal article
Metabolite Profiling Reveals Predictive Biomarkers and the Absence of beta-Methyl Amino-L-alanine in Plasma from Individuals Diagnosed with Amyotrophic Lateral Sclerosis
JOURNAL OF PROTEOME RESEARCH, 19(8), 3276–3285.
author keywords: amyotrophic lateral sclerosis; biomarkers; BMAA; CZE-MS/MS; metabolites; plasma
MeSH headings : Alanine; Amyotrophic Lateral Sclerosis / diagnosis; Biomarkers; Female; Humans; Male; Mass Spectrometry; Metabolome
TL;DR:
The plasma metabolome of 134 patients diagnosed with sporadic amyotrophic lateral sclerosis and 118 individuals deemed healthy was profiled, and two models using the metabolite abundances were developed for the separation of ALS and healthy samples and the prediction of disease progression based on the ALS functional rating score.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: September 14, 2020
2020 journal article
Methods for Cryosectioning and Mass Spectrometry Imaging of Whole-Body Zebrafish
Journal of the American Society for Mass Spectrometry, 31(4), 768–772.
author keywords: zebrafish; mass spectrometry imaging; cryosectioning; IR-MALDESI; lipids
MeSH headings : Animals; Cryoultramicrotomy / methods; Lipids / analysis; Male; Molecular Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Zebrafish / metabolism
TL;DR:
The zebrafish (Danio rerio) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease, and infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebra fish tissue sections.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: February 6, 2021
2020 journal article
Three-Dimensional Imaging with Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 31(2), 292–297.
author keywords: mass spectrometry imaging; 3D; IR-MALDESI; MUPS; HRAM
MeSH headings : Anti-Ulcer Agents / analysis; Citrates / analysis; Imaging, Three-Dimensional / methods; Omeprazole / analysis; Pharmaceutical Preparations / chemistry; Proton Pump Inhibitors / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Starch / analysis; Tablets, Enteric-Coated / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: March 10, 2020
2019 journal article
Artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua
Molecular Plant, 12(5), 704–714.
author keywords: Artemisia annua; artemisinin; glandular trichome; non-glandular trichome cell
MeSH headings : Artemisia annua / cytology; Artemisia annua / genetics; Artemisia annua / metabolism; Artemisia annua / physiology; Artemisinins / metabolism; Metabolic Engineering; Mutation; Pollination; Trichomes / metabolism
TL;DR:
It is reported that non-GT cells of self-pollinated inbred A. annua plants can express the artemisinin biosynthetic pathway and leaves of regenerated gl plantlets biosynthesize artemis inin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: February 24, 2020
2019 journal article
CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa
NEW PHYTOLOGIST, 222(1), 244–260.
author keywords: co-immunoprecipitation; enzyme activity; monolignol biosynthetic pathway; nuclear magnetic resonance (NMR); Populus trichocarpa; stem-differentiating xylem protein
MeSH headings : Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Down-Regulation / genetics; Gene Expression Regulation, Plant; Lignin / metabolism; Magnetic Resonance Spectroscopy; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Populus / genetics; Populus / metabolism; RNA Interference; Recombinant Proteins / metabolism; Xylem / metabolism
TL;DR:
Evidence is provided for the formation of PtrCAD1/PtrCCR2 protein complexes in monolignol biosynthesis in planta, and biomolecular fluorescence complementation and pull-down/co-immunoprecipitation experiments supported a hypothesis of P Tr trichocarpa heterodimer formation.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: December 17, 2018
2019 journal article
Discovery and quantification of bioactive peptides in fermented cucumber by direct analysis IR-MALDESI mass spectrometry and LC-QQQ-MS
FOOD CHEMISTRY, 271, 715–723.
author keywords: Lactic acid fermentation; ACE-inhibitory peptides; Isoleucine-proline-proline; Leucine-proline-proline; Valine-proline-proline; Vegetable composition
MeSH headings : Cucumis sativus / chemistry; Fermentation; Peptides / chemistry; Sodium Chloride; Tandem Mass Spectrometry
TL;DR:
It is demonstrated that lactic acid bacteria fermentation can enhance bioactive peptide content in vegetables by 3-5 fold in fermented cucumbers compared with acidified cucumbers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: October 16, 2018
2019 journal article
Discriminating normal regions within cancerous hen ovarian tissue using multivariate hyperspectral image analysis
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 33(4), 381–391.
MeSH headings : Animals; Chickens / metabolism; Female; Least-Squares Analysis; Multivariate Analysis; Ovarian Neoplasms / chemistry; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / pathology; Ovarian Neoplasms / veterinary; Ovary / chemistry; Ovary / pathology; Poultry Diseases / diagnosis; Poultry Diseases / pathology; Principal Component Analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Two unsupervised chemometric methods including PCA and MCR-ALS were applied for resolving and visualizing IR-MALDESI-MS data acquired from hen ovary tissues, improving the interpretation of mass spectrometry imaging results.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
10. Reduced Inequalities
(OpenAlex)
Sources: Web Of Science, ORCID
Added: February 18, 2019
2019 journal article
Heterogeneous antiretroviral drug distribution and HIV/SHIV detection in the gut of three species
SCIENCE TRANSLATIONAL MEDICINE, 11(499).
MeSH headings : ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism; Adolescent; Adult; Aged; Animals; Anti-Retroviral Agents / pharmacology; CD3 Complex / metabolism; Female; Gastrointestinal Tract / drug effects; Gastrointestinal Tract / virology; Gene Expression Regulation, Viral / drug effects; HIV / drug effects; HIV / genetics; Humans; Macaca mulatta; Male; Mice; Middle Aged; RNA, Viral / genetics; RNA, Viral / metabolism; Simian Immunodeficiency Virus / drug effects; Simian Immunodeficiency Virus / genetics; Species Specificity; T-Lymphocytes / drug effects; Young Adult
TL;DR:
There may be gut regions with little to no exposure to antiretroviral drugs, which may result in low-level HIV replication contributing to HIV persistence.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 5, 2019
2019 journal article
Internal Energy Deposition in Infrared Matrix-Assisted Laser Desorption Electrospray Ionization With and Without the Use of Ice as a Matrix
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 30(11), 2380–2391.
author keywords: Internal energy deposition; IR-MALDESI; Mass spectrometry imaging; Survival yield method; Thermometer ions; Ambient ionization
TL;DR:
Measurements with holo-myoglobin show that IR-MALDESI-MS retains the noncovalently bound heme-protein complexes under both native-like and denaturing conditions, while complete loss of the heme group occurred in denatured ESI-MS, showing that the softness of IR-MalDESI is equivalent or superior to ESI for biomolecules.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: November 25, 2019
2019 journal article
Label-Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress
PROTEOMICS, 19(14).
author keywords: drought; RNA regulation; Saccharum spp; shotgun proteomics; transcription factors
MeSH headings : Droughts; Gene Expression Regulation, Plant; Plant Proteins / metabolism; Proteomics; Saccharum / metabolism
TL;DR:
The integration of nuclei enrichment sample preparation with the shotgun proteomic approach results in great coverage of the sugarcane stem proteome with 5381 protein groups identified, indicating that drought affects negatively the cell wall metabolism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID
Added: July 22, 2019
2019 journal article
Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
ANALYTICAL METHODS, 11(46), 5929–5938.
MeSH headings : Animals; Diagnostic Imaging; Lasers; Mice; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR:
An effective strategy for direct analysis and two-dimensional matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) of mouse bones that underwent no chemical treatments prior to analysis is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: December 16, 2019
2019 journal article
NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods
Molecular & Cellular Proteomics, 19(1), 11–30.
author keywords: Glycomics; mass spectrometry; fluorescence; glycosylation; glycoproteins; glycan; glycopeptide; interlaboratory study; NISTmAb; reference antibody
MeSH headings : Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / metabolism; Biological Products; Biopharmaceutics / methods; Glycomics / methods; Glycopeptides / metabolism; Glycosylation; Humans; Laboratories; Polysaccharides / metabolism; Protein Processing, Post-Translational; Proteomics / methods
TL;DR:
A broad-based interlaboratory study of glycosylation profiles of a reference and modified IgG antibody involving 103 reports from 76 laboratories offers a view of the current state-of-the-art for biologic glyCosylation measurement and suggests a clear need for harmonization of gly cosylation analysis methods.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: May 11, 2020
2019 journal article
Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 26(25), 26039–26051.
author keywords: Root proteome; Cd stress; Antioxidant; Redox; Cell wall; Metabolism
MeSH headings : Cadmium / chemistry; Cadmium / metabolism; Cell Wall / metabolism; Drug Tolerance; Genotype; Solanum lycopersicum / metabolism; Plant Roots / metabolism; Proteome / chemistry; Proteome / metabolism; Proteomics; Stress, Physiological / drug effects
TL;DR:
Findings could shed some light on the molecular basis underlying the Cd stress response in tomato, providing fundamental insights for the development of Cd-safe cultivars.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
11. Sustainable Cities and Communities
(Web of Science)
Sources: Web Of Science, ORCID
Added: September 23, 2019
2019 journal article
Systematic evaluation of repeatability of IR-MALDESI-MS and normalization strategies for correcting the analytical variation and improving image quality
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 411(22), 5729–5743.
author keywords: IR-MALDESI; Mass spectrometry imaging; Repeatability; Normalization
MeSH headings : Animals; Chickens; Female; Rats; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Results demonstrated that the majority of normalization approaches benefit data quality to some extent, and local normalization methods significantly outperform their global counterparts, resulting in a reduction of median %RSD up to 22.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: September 9, 2019
2018 journal article
A novel integrated strategy for the detection and quantification of the neurotoxin beta-N-methylamino-l-alanine in environmental samples
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(10), 2597–2605.
author keywords: BMAA; CZE; Isomer separation; Quantification; HRAM MS/MS; Seafood
MeSH headings : Amino Acids, Diamino / analysis; Animals; Cyanobacteria Toxins; Environmental Monitoring / methods; Environmental Pollutants / analysis; Fishes / metabolism; Food Analysis / methods; Humans; Limit of Detection; Neurotoxins / analysis; Seafood / analysis; Tandem Mass Spectrometry / methods
TL;DR:
Interestingly, BMAA was not identified in the free protein fraction but only detected after protein hydrolysis which suggests that BMAA is tightly bound by and/or incorporated into proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2018 journal article
Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(9), 2395–2402.
author keywords: Mass spectrometry imaging; Infrared laser; IR-MALDESI; HRAM mass spectrometry
MeSH headings : Animals; Equipment Design; Infrared Rays; Lasers; Mice; Miniaturization / instrumentation; Optical Imaging / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Whole Body Imaging / instrumentation
TL;DR:
The use of a novel miniature 2.94 μm burst-mode laser in IR-MALDESI allows for rapid and sensitive mass spectrometry imaging of a whole mouse, yielding several hundred confident metabolite identifications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2018 journal article
Characterization of the Spectral Accuracy of an Orbitrap Mass Analyzer Using Isotope Ratio Mass Spectrometry
Analytical Chemistry, 90(3), 1897–1906.
MeSH headings : Caffeine / analysis; Carbon Isotopes / analysis; Nitrogen Isotopes / analysis; Oligopeptides / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Sulfur Isotopes / analysis
TL;DR:
It is demonstrated that at optimum absolute ions abundances the Orbitrap can be used to accurately count carbons, nitrogens, and sulfurs in samples with varying masses and absolute monoisotopic ion abundances required for high SA were empirically determined by using the expected and experimental isotopic distributions to calculate the Pearson chi-square test.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: February 21, 2020
2018 journal article
Demonstration of hydrazide tagging for O-glycans and a central composite design of experiments optimization using the INLIGHT (TM) reagent
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(5), 1409–1415.
author keywords: O-glycosylation; Hydrazide tag; Rapid derivatization; Design of experiments
MeSH headings : Chromatography, Liquid; Glycoproteins / chemistry; Hydrazines / chemistry; Mass Spectrometry; Polysaccharides / chemistry
TL;DR:
This work has taken the INLIGHT™ strategy for N-linked glycan derivatization and adopted it for use with O-linked glycans, demonstrating successful hydrazide tagging of O-glycans for the first time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2018 journal article
Evaluation of Digital Image Recognition Methods for Mass Spectrometry Imaging Data Analysis
Journal of The American Society for Mass Spectrometry, 29(12), 2467–2470.
author keywords: Mass spectrometry imaging; Image recognition; SSIM
MeSH headings : Algorithms; Animals; Image Processing, Computer-Assisted / methods; Lymph Nodes / chemistry; Lymph Nodes / metabolism; Macaca mulatta; Maraviroc / pharmacokinetics; Mass Spectrometry / methods; Models, Theoretical; Molecular Imaging / methods; Plant Leaves / chemistry; Tissue Distribution
TL;DR:
A method for comprehensive, semi-targeted discovery of molecular distributions of interest from mass spectrometry imaging data, using widely available image similarity scoring algorithms to rank images by spatial correlation is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: December 10, 2018
2018 journal article
IR-MALDESI mass spectrometry imaging of underivatized neurotransmitters in brain tissue of rats exposed to tetrabromobisphenol A
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(30), 7979–7986.
author keywords: IR-MALDESI; Neurotransmitters; Mass spectrometry imaging; Exposomics; Orbitrap
MeSH headings : Animals; Brain; Female; Infrared Rays; Neurotransmitter Agents / chemistry; Polybrominated Biphenyls / toxicity; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
This study serves as a demonstration of a new application of IR-MALDESI, namely that neurotransmitter distributions can be confidently and rapidly imaged without derivatization, and is used for the analysis of neurotransmitters in the brains of rats exposed to TBBPA in gestation and lactation through their mothers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: December 3, 2018
2018 journal article
IR-MALDESI method optimization based on time-resolved measurement of ion yields
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 410(3), 963–970.
author keywords: IR-MALDESI; Electrospray post-ionization; Mass spectrometry imaging; Laser ablation; Q Exactive
MeSH headings : Animals; Brain Chemistry; Cholesterol / analysis; Ions / analysis; Laser Therapy / methods; Lipids / analysis; Liver / chemistry; Mice; Rats; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
In a model imaging experiment using infrared matrix-assisted laser desorption electrospray ionization, fixing the injection time at the minimum duration required to capture all ions generated by the last laser pulse in a sequence is shown to maximize target ion abundances.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2018 journal article
Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis
Nature Communications, 9(1), 1579.
Contributors: J. Wang n , M. Matthews n, C. Williams n , R. Shi n, C. Yang n, S. Tunlaya-Anukit n, H. Chen n, Q. Li *
MeSH headings : Gene Expression Regulation, Plant; Lignin / biosynthesis; Lignin / genetics; Plants, Genetically Modified / genetics; Populus / genetics; Populus / metabolism; Transcriptome / genetics; Trees / genetics; Trees / metabolism; Wood / chemistry; Wood / physiology; Xylem / metabolism
TL;DR:
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels and may lead to greater understanding of other pathways for improved growth and adaptation.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2018 journal article
Jürgen H. Gross: Mass spectrometry: a textbook, 3rd ed.
Analytical and Bioanalytical Chemistry, 410(8), 2051–2052.
TL;DR:
To effectively make use of modern mass spectrometry for chemical and biological systems, one needs to understand the principles of the technique including ionization methods, mass analyzer technology, and ion dissociation as well as a basic understanding of isotopic distributions and measuring mass.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 21, 2020
2018 journal article
Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
ANALYST, 143(3), 654–661.
MeSH headings : Animals; Chickens; Chromatography, Liquid; Female; Glutathione / analysis; Ovarian Neoplasms / diagnostic imaging; Ovary / diagnostic imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry
TL;DR:
A ∼2-fold increase in the concentration of GSH in cancer tissue compared to the healthy tissue was observed using both techniques, and possible reasons for the difference between absolute concentration values obtained using IR-MALDESI QMSI and LC-MS/MS are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
Climate projections of spatial variations in coastal storm surges along the Gulf of Mexico and U.S. east coast
Journal of Ocean University of China, 16(1), 1–7.
author keywords: storm surge; sea surface winds; climate change; regional ocean
UN Sustainable Development Goal Categories
13. Climate Action
(Web of Science)
14. Life Below Water
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2017 journal article
Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae
Proteome Science, 15(1).
author keywords: Proteomics; Magnaporthe oryzae; Nitrogen starvation; Melanin biosynthesis; Extracellular protein
TL;DR:
A functional analysis of differentially expressed proteins revealed that during nitrogen starvation nitrogen catabolite repression, melanin biosynthesis, protein degradation and protein translation pathways underwent extensive alterations, consistent with observations of a link between nitrogen starvation and the development of pathogenicity in M. oryzae.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 6, 2018
2017 journal article
Cover Image
Rapid Communications in Mass Spectrometry, 31(22), i-i.
Sources: Crossref, ORCID
Added: February 21, 2020
2017 journal article
DRILL: An Electrospray Ionization-Mass Spectrometry Interface for Improved Sensitivity via Inertial Droplet Sorting and Electrohydrodynamic Focusing in a Swirling Flow
ANALYTICAL CHEMISTRY, 89(17), 8981–8987.
MeSH headings : Angiotensin I / analysis; Angiotensin I / metabolism; Angiotensin II / analysis; Angiotensin II / metabolism; Chromatography, High Pressure Liquid; Humans; Isotope Labeling; Limit of Detection; Peptides / analysis; Peptides / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
This work experimentally demonstrates sensitivity improvement obtained via vortex-induced inertial sorting of electrosprayed droplets/ions: one possible mode of DRILL operation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI
Analytical and Bioanalytical Chemistry, 410(3), 953–962.
author keywords: Terpenes; IR-MALDESI; SESI; Direct analysis; Biological buffers; Q Exactive Plus
MeSH headings : Alkenes / analysis; Buffers; Infrared Rays; Ions / analysis; Monocyclic Sesquiterpenes; Sesquiterpenes / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Terpenes / analysis
TL;DR:
By coupling the two techniques and doping the electrospray solvent with silver ions, olefinic terpenes of different classes and varying degrees of volatility were directly analyzed from a biological buffer system with no sample workup steps.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2017 journal article
Direct screening of enzyme activity using infrared matrix-assisted laser desorption electrospray ionization
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 31(22), 1868–1874.
MeSH headings : Drug Discovery / methods; Enzyme Assays / methods; High-Throughput Screening Assays / methods; Isocitrate Dehydrogenase / metabolism; Isocitrates / metabolism; Ketoglutaric Acids / metabolism; Models, Chemical; NADP / metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
The utility of infrared matrix-assisted laser desorption electrospray ionization for monitoring an enzymatic reaction directly from a biological buffer system with no sample cleanup and at high throughput is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
IR-MALDESI Mass Spectrometry Imaging at 50 Micron Spatial Resolution
Journal of The American Society for Mass Spectrometry, 28(10), 2099–2107.
author keywords: Mass spectrometry imaging; IR-MALDESI; Tissue imaging; Spatial resolution; Laser focusing
TL;DR:
This work presents MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) at 50 μm resolution, using an adjustable iris, beam expander, and an aspherical focusing lens to reduce tissue ablation diameters for MSI at high resolution.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2017 article
Imaging antiretroviral distribution within gastrointestinal tissues across pre-clinical species: implications for hiv eradication
Clinical Pharmacology & Therapeutics, Vol. 101, pp. S17–17.
Source: NC State University Libraries
Added: August 6, 2018
2017 journal article
MSiReader v1.0: Evolving Open-Source Mass Spectrometry Imaging Software for Targeted and Untargeted Analyses
Journal of The American Society for Mass Spectrometry, 29(1), 8–16.
author keywords: Mass spectrometry imaging; Mass spectrometry software; Data analysis; Image processing; High mass resolving power; Open-source; Orbitrap
TL;DR:
A major update to the mass spectrometry imaging (MSI) software MSiReader is presented, offering a multitude of newly added features critical to MSI analyses, enabling the MSI community to evaluate their data in greater depth and in less time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2017 journal article
Mass spectrometric detection of chlorophyll a and the tetrapyrrole secondary metabolite tolyporphin A in the filamentous cyanobacterium HT-58-2. Approaches to high-throughput screening of intact cyanobacteria
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, 21(11), 759–768.
author keywords: IR-MALDESI-FTMS; MS/MS; MALDI-TOF-MS; chlorophyll; bacteriochlorin; high-throughput screening
TL;DR:
Examination by MALDI-TOF-MS of lipophilic crude extracts of small-scale HT-58-2 samples without chromatographic fractionation enabled semi-quantitation of tolyporphin A over a 41-day growth period.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
N-linked glycosite profiling and use of Skyline as a platform for characterization and relative quantification of glycans in differentiating xylem of Populus trichocarpa
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 409(2), 487–497.
author keywords: N-linked glycosite; Skyline; Populus trichocarpa; Monolignol; N-linked glycans
MeSH headings : Computational Biology / standards; Computational Biology / trends; Polysaccharides / analysis; Populus / chemistry; Xylem / chemistry
TL;DR:
This study presents a novel workflow for identification of glycans using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy developed in the lab, and implements Skyline for relative glycan quantification for improved accuracy and precision of quantitative measurements over current processing tools.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
Perfluorinated alcohol induced coacervates as extraction media for proteomic analysis
JOURNAL OF CHROMATOGRAPHY A, 1523, 293–299.
author keywords: Coacervation; Surfactants; Perfluorinated alcohol; Proteomics; Membrane proteins
MeSH headings : Membrane Proteins / isolation & purification; Propanols / chemistry; Proteome / analysis; Proteomics / methods; Saccharomyces cerevisiae / metabolism; Surface-Active Agents / chemistry
TL;DR:
A novel method for using hexafluoroisopropanol induced coacervates with a variety of surfactants to extract proteins from a yeast whole cell lysate and conduct a global proteomic investigation on the extracted proteins is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 article
Recent advances in glycomics, glycoproteomics and allied topics
Mechref, Y., & Muddiman, D. C. (2017, January). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 409, pp. 355–357.
TL;DR:
The understanding of the attributes of glycans in biological functions and the development and progression of diseases has been facilitated by mass-spectrometry-based glycomics and glycoproteomics, which are dynamic and are rapidly evolving through innovative technologies and bioinformatics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
The PeptideAtlas of the Domestic Laying Hen
JOURNAL OF PROTEOME RESEARCH, 16(3), 1352–1363.
author keywords: biomarkers; chicken; ovarian cancer; PeptideAtlas
MeSH headings : Acute-Phase Proteins / analysis; Animals; Biomarkers, Tumor / analysis; Chickens; Data Mining; Female; Proteome / analysis; Proteomics / methods
TL;DR:
A PeptideAtlas for the domestic chicken is presented, containing an extensive and robust collection of chicken tissue and plasma samples with substantial value for the chicken proteomics community for protein validation and design of downstream targeted proteome quantitation workflows.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2017 journal article
The scope of Analytical and Bioanalytical Chemistry (ABC)
Analytical and Bioanalytical Chemistry, 410(3), 649–650.
TL;DR:
Assessing whether an application is within the scope of Analytical and Bioanalytical Chemistry, it is recommended that authors consider whether the major focus of the manuscript is to advance another field and whether the work demonstrates an innovative contribution to the field.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 21, 2020
2017 journal article
Xylose Migration During Tandem Mass Spectrometry of N-Linked Glycans
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 28(4), 729–732.
author keywords: Xylose migration; MS/MS; Glycomics; N-linked glycans; Plants
TL;DR:
This work reports the second instance of saccharide migration ever to occur for N-glycans, the first empirical observation of this rearrangement, and warrants dissemination so it may be searched in de novo sequencing glycan workflows.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
A Quantitative Glycomics and Proteomics Combined Purification Strategy
Journal of Visualized Experiments, 3(109).
author keywords: Chemistry; Issue 109; Glycomics; proteomics; glycosylation-site analysis; deamidation; hydrazide tagging; relative quantitation
MeSH headings : Animals; Chickens; Chromatography, Liquid / methods; Glycomics / methods; Glycopeptides / analysis; Glycopeptides / blood; Glycosylation; Humans; Mass Spectrometry / methods; Peptides / analysis; Peptides / blood; Polysaccharides / analysis; Polysaccharides / blood; Protein Processing, Post-Translational; Proteins / analysis; Proteomics / methods; Solid Phase Extraction / methods; Swine
TL;DR:
A new in-line high-throughput, unbiased, filter based protocol for quantitative glycomics and proteomics analysis and showed that N-glycans purified on filter and derivatized by hydrophobic hydrazide tags were comparable in terms of abundance and class to those by solid phase extraction (SPE); the latter is considered a gold standard in the field.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 6, 2018
2016 journal article
A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa
Tree Genetics & Genomes, 12(2).
author keywords: Lignin polymerization; Populus trichocarpa; Lignin peroxidase; LC-MS/MS; Lignin systems biology
TL;DR:
Differentially expressed gene (DEG) analysis of PtrPO21 downregulated transgenics identified a significant overexpression of PtPrx35, suggesting a compensatory effect within the peroxidase family.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2016 journal article
Analysis of Antiretrovirals in Single Hair Strands for Evaluation of Drug Adherence with Infrared-Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging
ANALYTICAL CHEMISTRY, 88(2), 1336–1344.
MeSH headings : Anti-Retroviral Agents / analysis; Hair / chemistry; Infrared Rays; Molecular Structure; Optical Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR:
Evaluated the detectability of HIV antiretrovirals in hair from a range of drug classes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) with 100 μm resolution and an MSI method to quantify the melanin biomarker pyrrole-2,3,5-tricarboxylic acid (PTCA) was evaluated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease
JOURNAL OF PROTEOME RESEARCH, 15(7), 2265–2282.
author keywords: Rosenthal fiber; Alexander disease; protein aggregates; neurodegenerative disease; astrocyte; proteomics; mass spectrometry (MS); stress granules
MeSH headings : Alexander Disease; Animals; Astrocytes; Cyclin D2 / analysis; DEAD-box RNA Helicases / analysis; GTP-Binding Proteins / analysis; Humans; Immunohistochemistry; Mice; Neoplasm Proteins / analysis; Neuropeptides / analysis; Protein Aggregates; Protein Aggregation, Pathological; Proteome / analysis; RNA Helicases / analysis; Receptors for Activated C Kinase; Receptors, Cell Surface / analysis
TL;DR:
No support was obtained for the sequestration hypothesis for AxD, but the association of several of the RF proteins with stress granules suggests a role for stressgranules in the origin of RFs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
Direct Analysis of Triterpenes from High-Salt Fermented Cucumbers Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Journal of The American Society for Mass Spectrometry, 28(2), 370–375.
author keywords: Food preservation; IR-MALDESI; Mass spectrometry imaging; Phytosterols; Direct analysis; HRAM; Fermented vegetable composition; Q Exactive
MeSH headings : Cucumis sativus / chemistry; Fermented Foods / analysis; Food Analysis / methods; Food Storage / methods; Image Processing, Computer-Assisted; Sodium Chloride; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry; Triterpenes / analysis; Triterpenes / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2016 journal article
Enhanced Lipidome Coverage in Shotgun Analyses by using Gas-Phase Fractionation
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(11), 1735–1744.
author keywords: Shotgun lipidomics; Gas-phase fractionation; Lipids; Hen model; Lipid metabolism; Polarity switching; Q Exactive Plus
MeSH headings : Animals; Chickens; Female; Glycerophospholipids; Ions; Lipids / chemistry; Tandem Mass Spectrometry
TL;DR:
This comprehensive analysis of the lipidome can serve as a species- and tissue-specific database for confident identification of other MS-based datasets, such as mass spectrometry imaging.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
Examining ubiquitinated peptide enrichment efficiency through an epitope labeled protein
ANALYTICAL BIOCHEMISTRY, 512, 114–119.
author keywords: Ubiquitination; Gly-Gly enrichment; Enrichment efficiency
MeSH headings : Antibodies, Monoclonal, Murine-Derived / chemistry; Epitopes / chemistry; Muramidase / chemistry; Ubiquitinated Proteins / chemistry; Ubiquitination
TL;DR:
It is shown that when conjugated to a lysine containing protein, such as lysozyme, it can be applied as a standard protein to examine ubiquitinated peptide enrichment within a complex background.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts
JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.
author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
TL;DR:
The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 review
Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens
[Review of ]. ANALYST, 141(18), 5236–5245.
MeSH headings : Histocytological Preparation Techniques; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TL;DR:
Challenges and limitations of MSI using IR-MALDESI are addressed in the context of the bioimaging field and valuable information is provided for targeted, label-free drug distribution studies and untargeted metabolomic datasets.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 chapter
MALDESI: Fundamentals, Direct Analysis, and MS Imaging
In Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry (pp. 169–182).
TL;DR:
The vast experimental space governing the MAL Desorption electrospray ionization process has been investigated thoroughly, while its applications in direct analysis of biomolecules were explored.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 18, 2021
2016 review
Optimizing Mass Spectrometry Analyses: A Tailored Review on the Utility of Design of Experiments
[Review of ]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(5), 767–785.
author keywords: Design of experiments; Mass spectrometry; Tutorial; Optimization; Modeling
MeSH headings : Mass Spectrometry; Models, Statistical; Research Design
TL;DR:
This review provides a tutorial-like guide into the selection of DOE for MS experiments, the practices for modeling and optimization of response variables, and the available software tools that support DOE implementation in any laboratory.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
ANALYST, 141(2), 595–605.
MeSH headings : Animals; Chickens; Female; Infrared Rays; Molecular Imaging / methods; Ovarian Neoplasms / diagnostic imaging; Ovarian Neoplasms / pathology; Ovary / cytology; Ovary / diagnostic imaging; Ovary / pathology; Solvents / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
In order to obtain a comprehensive metabolite and lipid data, a polarity switching MSI method using infrared matrix assisted laser desorption electrospray ionization (IR-MALDESI) was developed and optimized where the electrosPRay polarity was alternated from one voxel to the next.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
TransOmic analysis of forebrain sections in Sp2 conditional knockout embryonic mice using IR-MALDESI imaging of lipids and LC-MS/MS label-free proteomics
Analytical and Bioanalytical Chemistry, 408(13), 3453–3474.
author keywords: IR-MALDESI; Proteomics; Lipidomics; Mass spectrometry imaging; Embryonic brain
MeSH headings : Animals; Chromatography, Liquid / methods; Lipids / analysis; Mass Spectrometry / methods; Mice; Mice, Knockout; Prosencephalon / embryology; Prosencephalon / metabolism; Sp2 Transcription Factor / genetics; Spectrophotometry, Infrared / methods; Transcriptome
TL;DR:
An integrated approach for systematic analysis of the “lipidome” in tissue revealed many specific changes to fundamental cellular processes and biosynthetic pathways in the cerebral cortices of Sp2-cKO embryos.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2016 journal article
Wall modified photonic crystal fibre capillaries as porous layer open tubular columns for in-capillary micro-extraction and capillary chromatography
ANALYTICA CHIMICA ACTA, 905, 1–7.
author keywords: Polymer monolith; Porous layer open tubular column; Fused silica photonic crystal fibres; In-capillary extraction; Bioseparations
MeSH headings : Chromatography, Liquid / instrumentation; Chromatography, Liquid / methods; Equipment Design; Photons; Porosity; Reproducibility of Results; Spectrometry, Fluorescence
TL;DR:
The modified multi-channel capillaries were applied to the in-capillary micro-extraction of water samples, proving chromatographic potential of the new column format, with run-to-run retention time reproducibility of below 1%.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 article
What if you could only publish 50 papers your entire career?
Muddiman, D. C. (2016, January). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 408, pp. 663–664.
TL;DR:
Though this shift to the highest publishable unit (HPU) will not mean every paper is a blockbuster, it would guarantee that each manuscript fulfills one or more of three goals: Regardless of journal type or impact factor, each manuscript should detail a complete and significant study.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2016 journal article
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Journal of Visualized Experiments, 3(109).
author keywords: Bioengineering; Issue 109; Mass spectrometry imaging; IR-MALDESI; Tissue analysis; Whole-body imaging; Metabolites; Lipids
MeSH headings : Histocytological Preparation Techniques / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A robust IR-MALDESI imaging source has been developed, where a tunable mid-IR laser is synchronized with a computer controlled XY translational stage and a high resolving power mass spectrometer.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
4-Coumaroyl and Caffeoyl Shikimic Acids Inhibit 4-Coumaric Acid: Coenzyme A Ligases and Modulate Metabolic Flux for 3-Hydroxylation in Monolignol Biosynthesis of Populus trichocarpa
MOLECULAR PLANT, 8(1), 176–187.
author keywords: monolignol biosynthesis; Populus trichocarpa; metabolic flux; reaction and inhibition kinetics; LC-MS/MS; 4-coumaroyl and caffeoyl shikimic acids
MeSH headings : Acyl Coenzyme A / metabolism; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Hydroxylation / drug effects; Plant Proteins / metabolism; Populus / drug effects; Populus / enzymology; Populus / metabolism; Propionates; Shikimic Acid / pharmacology
TL;DR:
4-coumaroyl and caffeoyl shikimic acids are inhibitors of Ptr4CL3 and Ptr 4CL5, which can reduce lignin content in a number of plant species and play significant regulatory roles when these inhibitors accumulate.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts
JOURNAL OF BIOLOGICAL CHEMISTRY, 290(14), 8834–8848.
MeSH headings : Activins / metabolism; Base Sequence; Cell Differentiation; Cell Lineage; DNA Methylation; DNA Primers; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Ephrin-B2 / genetics; Humans; Nodal Protein / metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Signal Transduction; Trophoblasts / cytology; Trophoblasts / metabolism
TL;DR:
It is shown that activin/nodal signaling switches the terminal fate of these hESC-derived trophoblasts, providing a model system that allows for targeted derivation of extravillous trophoblast and syncytiotrophoblasts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Analysis of trace fibers by IR-MALDESI imaging coupled with high resolving power MS
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(3), 813–820.
author keywords: Mass spectrometry imaging; Forensic science; Trace evidence; Polarity switching imaging; MALDESI
TL;DR:
IR-MALDESI was used as a source for mass spectrometry imaging (MSI) of a dyed nylon fiber cluster and single fiber to yield more quantitative structural information about the dye and polymer that can be used for more conclusive comparisons.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Assessing drug and metabolite detection in liver tissue by UV-MALDI and IR-MALDESI mass spectrometry imaging coupled to FT-ICR MS
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 377, 448–455.
author keywords: MALDI; IR-MALDESI; Mass spectrometry imaging; Drug distribution
TL;DR:
The detection of lapatinib as well as several of its metabolites in liver tissue was determined by MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) coupled to high resolving power Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Cellular-level mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) by oversampling
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(8), 2265–2271.
author keywords: Mass spectrometry imaging; Infrared; MALDESI; Cellular analysis; Oversampling; Tissue analysis; Lipids
MeSH headings : Cells / chemistry; Cells / metabolism; Cervix Uteri / chemistry; Cervix Uteri / metabolism; Cholesterol / metabolism; Female; Humans; Microtomy; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) imaging of cervical tissue sections was performed using a spot-to-spot distance of 10 μm by utilizing the method of oversampling, where the target plate is moved by a distance that is less than the desOrption radius of the laser.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT Plasma N-Glycans
ANALYTICAL CHEMISTRY, 87(14), 7305–7312.
MeSH headings : Animals; Chickens; Equipment Design; Mass Spectrometry / instrumentation; Polysaccharides / blood; Polysaccharides / isolation & purification; Solid Phase Extraction / instrumentation
TL;DR:
High-throughput, quantitative processing of N-linked glycans would facilitate large-scale studies correlating the glycome with disease and open the field to basic and applied researchers by coupling filter-aided-N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags (INLIGHT) for analysis of plasma.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Elucidation of Xylem-Specific Transcription Factors and Absolute Quantification of Enzymes Regulating Cellulose Biosynthesis in Populus trichocarpa
JOURNAL OF PROTEOME RESEARCH, 14(10), 4158–4168.
Contributors: P. Loziuk n, J. Parker n, W. Li n, C. Lin n, J. Wang n , Q. Li *, R. Sederoff n, V. Chiang n, n
author keywords: shotgun discovery proteomics; absolute quantification; PC-IDMS; cellulose biosynthesis; transcription factor; targeted mass spectrometiry; SRM
MeSH headings : Carbohydrate Metabolism; Cellulose / biosynthesis; Cellulose / genetics; Chromatography, Liquid; Gene Expression Regulation, Plant; Molecular Sequence Annotation; Plant Proteins / genetics; Plant Proteins / isolation & purification; Plant Proteins / metabolism; Populus / genetics; Populus / metabolism; Proteome / genetics; Proteome / isolation & purification; Proteome / metabolism; Proteomics; Tandem Mass Spectrometry; Transcription Factors / genetics; Transcription Factors / isolation & purification; Transcription Factors / metabolism; Wood / chemistry; Wood / metabolism; Xylem / genetics; Xylem / metabolism
TL;DR:
The study described herein sought to identify the proteins directly involved in cellulose biosynthesis during wood formation in Populus trichocarpa along with known xylem-specific transcription factors involved in regulating these key proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Global Proteomic Analysis of Functional Compartments in Immature Avian Follicles Using Laser Microdissection Coupled to LC-MS/MS
JOURNAL OF PROTEOME RESEARCH, 14(9), 3912–3923.
author keywords: microdissection; proteomics; ovary; follicle; avian; chicken; yolk; steroidogenesis; vitellogenin
MeSH headings : Animals; Avian Proteins / analysis; Avian Proteins / chemistry; Chickens; Chromatography, Liquid; Female; Laser Capture Microdissection / methods; Ovarian Follicle / chemistry; Proteome / analysis; Proteome / chemistry; Proteomics / methods; Tandem Mass Spectrometry
TL;DR:
A global proteome for SWF, white yolk, and ovarian stroma of the avian ovary is provided that can be used as a baseline for future studies and verifies that the coupling of LMD with proteomic analysis can be use to evaluate proteins from small, physiologically functional compartments of complex tissue.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
In-depth LC-MS/MS analysis of the chicken ovarian cancer proteome reveals conserved and novel differentially regulated proteins in humans
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(22), 6851–6863.
author keywords: Mass spectrometry; Proteomics; Ovarian Cancer; Chicken; Ovostatin 2; OVOS2
MeSH headings : Amino Acid Sequence; Animals; Chickens; Chromatography, Liquid / methods; Conserved Sequence; Female; Gene Expression Regulation, Neoplastic; Humans; Mass Spectrometry / methods; Molecular Sequence Data; Neoplasm Proteins / metabolism; Ovarian Neoplasms / metabolism; Proteome / chemistry; Proteome / metabolism; Species Specificity
TL;DR:
Analysis of the ovarian cancer proteome of the chicken provides the first evidence for involvement of OVOS2 in the pathogenesis of both chicken and human ovarian cancer.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Influence of C-Trap Ion Accumulation Time on the Detectability of Analytes in IR-MALDESI MSI
ANALYTICAL CHEMISTRY, 87(20), 10483–10490.
MeSH headings : Ions / chemistry; Lasers; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Infrared; Time Factors; Xenobiotics / analysis
TL;DR:
Reducing the C-trap ion accumulation time resulted in increased ion abundance by up to 3 orders of magnitude for analytes ranging from xenobiotics to endogenous lipids, and facilitated the reduction of voxel-to-voxel variability by more than 3-fold.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Influence of Desorption Conditions on Analyte Sensitivity and Internal Energy in Discrete Tissue or Whole Body Imaging by IR-MALDESI
Journal of The American Society for Mass Spectrometry, 26(6), 899–910.
author keywords: IR-MALDESI; Mass spectrometry imaging; Laser desorption whole body imaging; Lipids; Xenobiotics
MeSH headings : Anesthetics, Local / analysis; Anesthetics, Local / pharmacokinetics; Animals; Cocaine / analysis; Cocaine / pharmacokinetics; Infrared Rays; Lipid Metabolism; Lipids / analysis; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Whole Body Imaging / methods
TL;DR:
Optimized desorption conditions have been used to conduct an untargeted lipidomic analysis of whole body sagittal sections of neonate mouse, with enhanced lipid coverage received by varying the laser wavelength used for mass spectrometry imaging (MSI).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2015 journal article
Machine learning reveals sex-specific 17β-estradiol-responsive expression patterns in white perch (Morone americana) plasma proteins
PROTEOMICS, 15(15), 2678–2690.
Contributors: J. Schilling n, A. Nepomuceno n, A. Planchart n , J. Yoder n , R. Kelly n , n , H. Daniels n, N. Hiramatsu*, B. Reading n
author keywords: Animal proteomics; Endocrine disrupting compounds; Machine learning; Plasma; Semiquantitative proteomics; Support vector machines
MeSH headings : Animals; Bass / metabolism; Blood Proteins / metabolism; Blotting, Western; Chromatography, Liquid; Estradiol / pharmacology; Estrogens / pharmacology; Female; Fish Proteins / metabolism; Kininogens / metabolism; Male; Nanotechnology / methods; Proteome / drug effects; Proteome / metabolism; Proteomics / methods; Sex Factors; Support Vector Machine; Tandem Mass Spectrometry; Vitellogenins / metabolism
TL;DR:
This is the first report that Eltd1 and Kng1 may be E2‐responsive proteins in fishes and therefore may be useful indicators of estrogen induction in fishes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2015 journal article
Mass Spectrometry Imaging Reveals Heterogeneous Efavirenz Distribution within Putative HIV Reservoirs
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 59(5), 2944–2948.
MeSH headings : Alkynes; Animals; Anti-Retroviral Agents / pharmacokinetics; Benzoxazines / pharmacokinetics; Cyclopropanes; Gray Matter / metabolism; Lymph Nodes / metabolism; Macaca; Mass Spectrometry / methods
TL;DR:
Intratissue efavirenz distribution was heterogeneous, with the drug concentrating in the lamina propria of the colon, the primary follicles of lymph nodes, and the brain gray matter, the first imaging data of an antiretroviral drug in active viral reservoirs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Mechanisms of Egg Yolk Formation and Implications on Early Life History of White Perch (Morone americana)
PLOS ONE, 10(11).
MeSH headings : Animals; Bass / metabolism; Blotting, Western; Chromatography, Liquid; Egg Proteins / metabolism; Egg Yolk / metabolism; Female; Immunohistochemistry; Oocytes / cytology; Oocytes / metabolism; Ovary / cytology; Receptors, Cell Surface / metabolism; Sexual Maturation; Staining and Labeling; Tandem Mass Spectrometry; Vitellogenins / metabolism
TL;DR:
The three white perch vitellogenins were quantified accurately and precisely in the liver, plasma, and ovary during pre-vitellogenic oocyte growth using protein cleavage-isotope dilution mass spectrometry (PC-IDMS).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 personal communication
Michael Gross: 25 Years of Dedication and Leadership of JASMS (1990-2015)
Muddiman, D. C. (2015, January).
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Phosphoproteome Analysis Links Protein Phosphorylation to Cellular Remodeling and Metabolic Adaptation during Magnaporthe oryzae Appressorium Development
Journal of Proteome Research, 14(6), 2408–2424.
author keywords: Magnaporthe oryzae; quantitative phosphoproteomics; appressorium formation; network analysis; transcription factors
MeSH headings : Adaptation, Physiological; Basic Helix-Loop-Helix Transcription Factors / metabolism; Chromatography, Liquid; Fungal Proteins / metabolism; Magnaporthe / metabolism; Magnaporthe / physiology; Oryza / microbiology; Phosphoproteins / metabolism; Phosphorylation; Proteomics; Signal Transduction; Tandem Mass Spectrometry
TL;DR:
Network analysis incorporating regulation from transcriptomic, proteomic, and phosphoproteomic data revealed new insights into the regulation of the metabolism of conidial storage reserves and phospholipids, autophagy, actin dynamics, and cell wall metabolism during appressorium formation.
(via Semantic Scholar)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2015 journal article
Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis
Proceedings of the National Academy of Sciences, 112(27), 8481–8486.
author keywords: AldOMT; COMT; lignin; phosphoproteomics; phosphoregulation
MeSH headings : Acrolein / analogs & derivatives; Acrolein / metabolism; Amino Acid Sequence; Binding Sites / genetics; Biocatalysis; Catechols / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Isoenzymes / genetics; Isoenzymes / metabolism; Lignin / biosynthesis; Methyltransferases / genetics; Methyltransferases / metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutation; Phosphoproteins / metabolism; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Populus / enzymology; Populus / genetics; Populus / metabolism; Proteomics / methods; Protoplasts / enzymology; Protoplasts / metabolism; Sequence Homology, Amino Acid; Tandem Mass Spectrometry
TL;DR:
It is shown that reversible monophosphorylation at Ser123 or Ser125 acts as an on/off switch for the activity of 5-hydroxyconiferaldehyde O-methyltransferase 2 (PtrAldOMT2), an enzyme central to monolignol biosynthesis for lignification in stem-differentiating xylem of Populus trichocarpa.
(via Semantic Scholar)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2015 journal article
Quantitative mass spectrometry imaging of emtricitabine in cervical tissue model using infrared matrix-assisted laser desorption electrospray ionization
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(8), 2073–2084.
author keywords: Mass spectrometry imaging; IR-MALDESI; Absolute quantification; Drug distribution; HIV; Selected reaction monitoring
MeSH headings : Diagnostic Imaging / instrumentation; Diagnostic Imaging / methods; Female; Humans; Papillomavirus Vaccines / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Uterine Cervical Neoplasms / chemistry; Uterine Cervical Neoplasms / diagnosis
TL;DR:
A quantitative mass spectrometry imaging technique using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is demonstrated for the antiretroviral (ARV) drug emtricitabine in incubated human cervical tissue, leading to a gain in sensitivity and removal of interferences for several ARV drugs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2015 journal article
Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples
JOURNAL OF PROTEOME RESEARCH, 14(10), 4394–4401.
author keywords: N-linked glycosylation; INLIGHT; ovarian cancer; relative quantification; cancer biomarker; human plasma
MeSH headings : Biomarkers, Tumor / blood; Carbohydrate Sequence; Case-Control Studies; Chromatography, Liquid / methods; Female; Fucose / blood; Glycosylation; Humans; Hydrazines / chemistry; Molecular Sequence Data; Neoplasm Staging; Ovarian Neoplasms / blood; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / pathology; Polysaccharides / blood; Sialic Acids / blood; Staining and Labeling / methods; Tandem Mass Spectrometry / methods
TL;DR:
For all N-glycans, the vectors of the effects from stages II-IV were sequentially reversed, suggesting potential biological changes in OVC morphology or in host response.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Accurate Identification of Deamidated Peptides in Global Proteomics Using a Quadrupole Orbitrap Mass Spectrometer
JOURNAL OF PROTEOME RESEARCH, 13(2), 777–785.
author keywords: deamidation; post-translational modification; quadrupole orbitrap mass spectrometry
MeSH headings : Amides / chemistry; Chromatography, Liquid; Peptides / chemistry; Proteomics; Tandem Mass Spectrometry / instrumentation
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 article
Are Presentations at ASMS Conferences Publications?
Muddiman, D. C., & Weintraub, S. T. (2014, March). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 25, pp. 301–302.
MeSH headings : Congresses as Topic; Humans; Mass Spectrometry; Publications; Societies, Scientific / organization & administration
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 chapter
Atmospheric Pressure Mass Spectrometry Imaging
In R. A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation (pp. 1–44).
Ed(s): R. Meyers
Sources: NC State University Libraries, ORCID
Added: March 8, 2021
2014 journal article
C/EBPα regulates CRL4Cdt2-mediated degradation of p21 in response to UVB-induced DNA damage to control the G1/S checkpoint
Cell Cycle, 13(22), 3602–3610.
author keywords: C/EBP alpha; CRL4(Cdt2); G(1)/S DNA damage checkpoint; keratinocytes; p21
MeSH headings : Animals; CCAAT-Enhancer-Binding Protein-alpha / genetics; CCAAT-Enhancer-Binding Protein-alpha / metabolism; Cell Cycle Checkpoints / genetics; Cell Cycle Checkpoints / radiation effects; Cyclin-Dependent Kinase Inhibitor p21 / metabolism; DNA Damage / genetics; DNA Damage / radiation effects; G1 Phase Cell Cycle Checkpoints / genetics; Keratinocytes / metabolism; Keratinocytes / radiation effects; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Proteolysis; Ubiquitin-Protein Ligases / genetics; Ubiquitin-Protein Ligases / metabolism; Ultraviolet Rays; p21-Activated Kinases / genetics; p21-Activated Kinases / metabolism
TL;DR:
Findings reveal C/EBPα regulates G1/S cell cycle arrest in response to DNA damage via the control of CRL4Cdt2 mediated degradation of p21.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2014 journal article
Compartment Proteomics Analysis of White Perch (Morone americana) Ovary Using Support Vector Machines
JOURNAL OF PROTEOME RESEARCH, 13(3), 1515–1526.
author keywords: compartment proteomics; support vector machines; modulated modularity clustering; ovary; oocytes; vitellogenin; transcriptome; mitochondria; alternatively spliced variants
MeSH headings : Animals; Bass / metabolism; Chemical Fractionation; Female; Fish Proteins / analysis; Membrane Proteins / analysis; Mitochondrial Proteins / analysis; Molecular Sequence Annotation; Ovary / chemistry; Ovary / metabolism; Proteomics / methods; Support Vector Machine; Tandem Mass Spectrometry
TL;DR:
The white perch ovary cytosolic and membrane proteomes and transcriptome presented in this study can support future investigations into oogenesis and early embryogenesis ofwhite perch and other members of the genus Morone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa
The Plant Cell, 26(3), 894–914.
MeSH headings : Kinetics; Lignin / metabolism; Mass Spectrometry; Plant Proteins / metabolism; Polymorphism, Single Nucleotide; Populus / metabolism; Proteome
TL;DR:
A proteomic-based predictive kinetic metabolic-flux model was developed for monolignol biosynthesis in Populus trichocarpa and predicts how pathway enzymes affect lignin content and composition, explains a long-standing paradox regarding the regulation of monolIGNol subunit ratios in lignIn, and reveals novel mechanisms involved in theregulation of lign in biosynthesis.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2014 journal article
Establishing ion ratio thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry
ANALYST, 139(21), 5439–5450.
MeSH headings : Chromatography, Liquid; Isotopes / chemistry; Proteins / analysis; Tandem Mass Spectrometry / methods
TL;DR:
It is suggested that evaluation of the absolute ion abundance for any given transition is necessary in order to effectively implement RA thresholds, and these RA values should be assessed as close as possible to the time at which data is collected for quantification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis
Rapid Communications in Mass Spectrometry, 28(23), 2511–2522.
MeSH headings : Acyltransferases / metabolism; Chromatography, Liquid / methods; Filtration; Hydrophobic and Hydrophilic Interactions; Mass Spectrometry / methods; Oligopeptides / chemistry; Oligopeptides / metabolism; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Polyketides / chemistry; Polyketides / metabolism
TL;DR:
The MS signal as a function of nonpolar surface area (NPSA) is explored in order to better understand this bias in electrospray response and to determine relative LC/MS response for peptides.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2014 article
Focus on Advancing High Performance Mass Spectrometry, Honoring Dr. Richard D. Smith, Recipient of the 2013 Award for a Distinguished Contribution in Mass Spectrometry
Baker, E. S., Muddiman, D. C., & Loo, J. A. (2014, December). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 25, pp. 1997–1999.
TL;DR:
This special focus issue of the Journal of the American Society for Mass spectrometry celebrates the accomplishments of Dr. Richard D. Smith, the recipient of the 2013ASMS Award for a Distinguished Contribution in Mass Spectrometry.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Hierarchical Self-Assembly of Supramolecular Hydrophobic Metallacycles into Ordered Nanostructures
CHEMISTRY-AN ASIAN JOURNAL, 9(10), 2928–2936.
author keywords: metallacycles; noncovalent interactions; nanostructures; self-assembly; supramolecular chemistry
TL;DR:
A new series of well-defined metallacycles decorated with long alkyl chains were obtained through metal-ligand interactions, which were capable of aggregating into ordered fibroid or spherical nanostructures on the surface, mostly driven by hydrophobic interactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
IR-MALDESI Mass Spectrometry Imaging of Biological Tissue Sections Using Ice as a Matrix
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 25(3), 319–328.
author keywords: Mass spectrometry imaging; IR-MALDESI; Tissue ablation; Shadowgraphy
MeSH headings : Animals; Brain / cytology; Brain Chemistry; Histocytological Preparation Techniques / methods; Ice; Mice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
With a better understanding of the fundamentals of ablation dynamics and a systematic approach to explore the experimental space, it was possible to improve ion abundance by nearly one order of magnitude.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Mapping Antiretroviral Drugs in Tissue by IR-MALDESI MSI Coupled to the Q Exactive and Comparison with LC-MS/MS SRM Assay
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 25(12), 2038–2047.
author keywords: IR-MALDESI; Mass spectrometry imaging; LC-MS/MS; Drug distribution
MeSH headings : Adenine / analogs & derivatives; Adenine / analysis; Adenine / chemistry; Adenine / pharmacokinetics; Anti-Retroviral Agents / analysis; Anti-Retroviral Agents / chemistry; Anti-Retroviral Agents / pharmacokinetics; Cervix Uteri / chemistry; Cervix Uteri / metabolism; Chromatography, Liquid / methods; Deoxycytidine / analogs & derivatives; Deoxycytidine / analysis; Deoxycytidine / chemistry; Deoxycytidine / pharmacokinetics; Emtricitabine; Female; Histocytochemistry; Humans; Molecular Imaging / methods; Organophosphonates / analysis; Organophosphonates / chemistry; Organophosphonates / pharmacokinetics; Pyrrolidinones / analysis; Pyrrolidinones / chemistry; Pyrrolidinones / pharmacokinetics; Raltegravir Potassium; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Tandem Mass Spectrometry / methods; Tenofovir; Tissue Distribution
TL;DR:
An agreement between the average intensities from the imaging experiment and the absolute quantities for each drug was observed and this correlation between these two techniques serves as a prerequisite to quantitative IR-MALDESI MSI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 article
Reply to the Comment on: "Utilizing Artificial Neural Networks in MATLAB to Achieve Parts-Per-Billion Mass Measurement Accuracy with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer" by D. Keith Williams Jr., Alexander L. Kovach, David C. Muddiman, and Kenneth W. Hanck. J-Am. Soc. Mass Spectrom. 20, 1303-1310 (2009)
Muddiman, D. C. (2014, April). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol. 25, pp. 697–697.
MeSH headings : Fourier Analysis; Mass Spectrometry / methods; Neural Networks, Computer
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Silver dopants for targeted and untargeted direct analysis of unsaturated lipids via infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 28(22), 2461–2470.
MeSH headings : Alkenes / chemistry; Dried Blood Spot Testing; Fats, Unsaturated / analysis; Fats, Unsaturated / chemistry; Humans; Male; Silver / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
Silver cationization has been shown to enhance IR-MALDESI sensitivity and selectivity for unsaturated lipids, even when applied to complex samples, and developed tools for peak assignment and mapping of isotopic patterns will clearly benefit future mass spectrometry imaging studies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2014 journal article
Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling
The Plant Cell, 26(3), 876–893.
Contributors: H. Chen n, J. Song n, J. Wang n , Y. Lin n, J. Ducoste n , C. Shuford n, J. Liu n, Q. Li n
MeSH headings : Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Immunoprecipitation; Lignin / biosynthesis; Mass Spectrometry; Models, Biological; Populus / metabolism; Propionates; RNA, Messenger / genetics; Substrate Specificity; Systems Biology
TL;DR:
This work shows that 4CL, an enzyme in monolignol biosynthesis, is found as a heterotetrameric complex of two isoforms in Populus trichocarpa, where the two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact in vivo and in vitro to form a heterosymmetric protein complex.
(via Semantic Scholar)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2013 journal article
Comparative Proteomic Analysis and IgE Binding Properties of Peanut Seed and Testa (Skin)
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61(16), 3957–3968.
author keywords: IgE binding; LC-MS/MS; peanut; proteomics; skins; testae
MeSH headings : Allergens / immunology; Antigens, Plant / immunology; Arachis / chemistry; Arachis / immunology; Humans; Immunoglobulin E / metabolism; Peanut Hypersensitivity / immunology; Plant Proteins / analysis; Plant Proteins / chemistry; Plant Proteins / immunology; Proteomics; Seeds / chemistry; Seeds / immunology
TL;DR:
Findings indicate that peanut skins contain potentially allergenic proteins; however, the presence of phenolic compounds may attenuate this effect.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Direct Analysis of Textile Fabrics and Dyes Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry
ANALYTICAL CHEMISTRY, 85(2), 831–836.
MeSH headings : Coloring Agents / analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Textiles / analysis
TL;DR:
A direct analysis of dyed textile fabric was performed using the infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source for MS, allowing the omission of the lengthy sample preparation involved in extraction, thereby significantly reducing the overall analysis time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Factorial Experimental Designs Elucidate Significant Variables Affecting Data Acquisition on a Quadrupole Orbitrap Mass Spectrometer
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 24(10), 1501–1512.
author keywords: Design of experiments; Data dependent acquisition; Proteome coverage; Q Exactive; FTMS
MeSH headings : Models, Chemical; Proteome / analysis; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT): A Novel Glycan-Relative Quantification Strategy
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 24(9), 1376–1384.
author keywords: Glycomics; Hydrazide Reagents; Stable Isotope Labeling; Normalization; Hydrazone; Formation; Relative Quantification; N-Linked Glycans
MeSH headings : Chromatography, Reverse-Phase; Glycomics / methods; Glycoproteins / chemistry; Glycosylation; Humans; Isotope Labeling / methods; Mass Spectrometry / methods; Plasma / chemistry; Polysaccharides / analysis; Polysaccharides / isolation & purification
TL;DR:
The ability to spike in a glycoprotein and detect change at the glycan level validates both the sample preparation and data analysis strategy, making INLIGHT an invaluable relative quantification strategy for the field of glycomics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Imaging Source Coupled to a FT-ICR Mass Spectrometer
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 24(1), 92–100.
author keywords: FTMS; Mass spectrometry imaging; Infrared laser; MALDESI; Atmospheric pressure
MeSH headings : Animals; Atmospheric Pressure; Brain Chemistry; Fourier Analysis; Lipids / analysis; Lipids / chemistry; Lung / chemistry; Mice; Molecular Imaging / methods; Myocardium / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
This work introduces here a versatile source for MALDESI imaging analysis coupled to a hybrid LTQ-FT-ICR mass spectrometer, offering single shot or multi-shot capability per pixel with full control over the laser repetition rate and mass Spectrometer scanning cycle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
MSiReader: An Open-Source Interface to View and Analyze High Resolving Power MS Imaging Files on Matlab Platform
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 24(5), 718–721.
author keywords: Mass spectrometry imaging; Software; High mass resolving power; FT-ICR-MS; Open-source
MeSH headings : Algorithms; Computational Biology; Image Processing, Computer-Assisted / methods; Internet; Mass Spectrometry / methods; Software; User-Computer Interface
TL;DR:
MSiReader is introduced, a free open source application to read and analyze high resolution MSI data from the most common MSi data formats and is built on the Matlab platform and includes a large selection of data analysis tools and features.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Mass Recalibration of FT-ICR Mass Spectrometry Imaging Data Using the Average Frequency Shift of Ambient Ions
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 24(7), 1137–1145.
author keywords: Mass spectrometry imaging; FT-ICR MS; Mass calibration; MALDESI; MMA
MeSH headings : Animals; Brain / anatomy & histology; Brain Chemistry; Calibration; Cyclotrons; Fourier Analysis; Infrared Rays; Ions; Mass Spectrometry / methods; Mass Spectrometry / statistics & numerical data; Mice; Molecular Imaging / methods; Molecular Imaging / statistics & numerical data; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Electrospray Ionization / statistics & numerical data; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / statistics & numerical data
TL;DR:
A calibration method is applied based on the observed cyclotron frequency shift of ions found in the ambient laboratory environment, polydimethylcyclosiloxanes (PDMS) to achieve part per billion mass accuracy with relatively high precision for an infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) MSI dataset.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Monolignol Pathway 4-Coumaric Acid: Coenzyme A Ligases in Populus trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes
PLANT PHYSIOLOGY, 161(3), 1501–1516.
Contributors: H. Chen n, J. Song n, C. Williams n , C. Shuford n, J. Liu n, J. Wang n , Q. Li n, R. Shi n
MeSH headings : Allosteric Regulation / drug effects; Binding Sites; Biosynthetic Pathways / drug effects; Blotting, Western; Caffeic Acids / pharmacology; Coenzyme A / metabolism; Coenzyme A Ligases / antagonists & inhibitors; Coenzyme A Ligases / metabolism; Computer Simulation; Coumaric Acids / chemistry; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Kinetics; Lignin / biosynthesis; Lignin / chemistry; Phenylpropionates / metabolism; Phosphoproteins / metabolism; Phosphorylation / drug effects; Plant Extracts; Populus / drug effects; Populus / enzymology; Propionates; Proteomics; Recombinant Fusion Proteins / metabolism; Sequence Homology, Amino Acid; Substrate Specificity / drug effects; Xylem / drug effects; Xylem / metabolism
TL;DR:
Evidence is presented of two monolignol 4CLs, Ptr4CL3 and P tr4CL5, in Populus trichocarpa that act to mitigate the inhibition by caffeic acid to maintain a proper ligation flux and coenzyme A flux with novel specificity.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Multi-peptide nLC-PC-IDMS-SRM-based Assay for the quantification of biomarkers in the chicken ovarian cancer model
Methods, 61(3), 323–330.
author keywords: Proteomics; Quantification; Ovarian cancer; Chicken; Biomarkers
MeSH headings : Amino Acid Sequence; Animals; Biological Assay; Biomarkers, Tumor / analysis; Biomarkers, Tumor / blood; Biomarkers, Tumor / chemistry; Calibration; Carbon Isotopes; Chickens; Disease Models, Animal; Female; Humans; Indicator Dilution Techniques; Isotope Labeling; Molecular Sequence Data; Nitrogen Isotopes; Ovarian Neoplasms / chemistry; Ovarian Neoplasms / metabolism; Peptide Fragments / analysis; Prealbumin / analysis; Prealbumin / chemistry; Prealbumin / metabolism; Proteomics / methods; Proteomics / standards; Tandem Mass Spectrometry / methods; Tandem Mass Spectrometry / standards; Vitellogenins / analysis; Vitellogenins / blood; Vitellogenins / chemistry; alpha-Macroglobulins / analysis; alpha-Macroglobulins / chemistry; alpha-Macroglobulins / metabolism
TL;DR:
These data illustrate the successes and challenges of qualifying quantitative levels of proteins from an in-gel digestion sample preparation followed by LC-MS/MS (GeLC) label-free discovery-based approach to a targeted SRM-based quantitative assay in plasma and tissues.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2013 journal article
Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa
Planta, 238(3), 487–497.
Contributors: R. Shi n, C. Shuford n, J. Wang n , Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n
MeSH headings : Gene Expression Regulation, Plant / physiology; Mass Spectrometry; Phenylalanine Ammonia-Lyase / metabolism; Populus / enzymology; Populus / metabolism
TL;DR:
A greater functional significance of subgroup A PtrPALs for stem wood formation is indicated, and the role of gene post-transcriptional regulation is highlighted.
(via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2013 journal article
Temporal Analysis of theMagnaporthe OryzaeProteome During Conidial Germination and Cyclic AMP (cAMP)-mediated Appressorium Formation
Molecular & Cellular Proteomics, 12(8), 2249–2265.
MeSH headings : Cyclic AMP / metabolism; Fungal Proteins / metabolism; Magnaporthe / growth & development; Magnaporthe / metabolism; Mitochondrial Proteins / metabolism; Peptide Hydrolases / metabolism; Proteome; Spores, Fungal / growth & development; Spores, Fungal / metabolism
TL;DR:
The data presented here provide the first comprehensive view of the M. oryzae proteome during early infection-related development and highlight biological processes important for pathogenicity.
(via Semantic Scholar)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2013 journal article
The use of a xylosylated plant glycoprotein as an internal standard accounting for N-linked glycan cleavage and sample preparation variability
Rapid Communications in Mass Spectrometry, 27(12), 1354–1358.
MeSH headings : Analytic Sample Preparation Methods / standards; Glycoproteins / chemistry; Glycosylation; Mass Spectrometry / standards; Plant Proteins / chemistry; Polysaccharides / chemistry; Protein Processing, Post-Translational; Reference Standards
TL;DR:
The inclusion of two internal standards, maltoheptaose and horseradish peroxidase (HRP), is studied to demonstrate the effectiveness of using a glycoprotein as an internal standard in glycan relative quantification experiments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2013 journal article
Understanding the Role of Proteolytic Digestion on Discovery and Targeted Proteomic Measurements Using Liquid Chromatography Tandem Mass Spectrometry and Design of Experiments
JOURNAL OF PROTEOME RESEARCH, 12(12), 5820–5829.
Contributors: P. Loziuk n, J. Wang n , Q. Li n, R. Sederoff n, V. Chiang n & n
author keywords: design of experiments; proteolytic digestion; LC-MS/MS; Populus trichocarpa
MeSH headings : Amino Acid Sequence; Animals; Cattle; Chromatography, Liquid / standards; Factor Analysis, Statistical; Hydrogen-Ion Concentration; Isotope Labeling; Molecular Sequence Annotation; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / isolation & purification; Plant Proteins / chemistry; Plant Proteins / isolation & purification; Populus / chemistry; Protein Stability; Proteolysis; Proteomics / methods; Proteomics / standards; Research Design; Swine; Tandem Mass Spectrometry / standards; Trypsin / chemistry; Xylem / chemistry
TL;DR:
The tryptic digest optimization described here has eliminated this previously observed peptide decay as well as provided a greater understanding and standardization for a common but critical sample treatment used across the field of proteomics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
Alexander R. Ivanov, Alexander V. Lazarev (Eds): Sample preparation in biological mass spectrometry
Analytical and Bioanalytical Chemistry, 404(5), 1331–1332.
Sources: Crossref, ORCID
Added: January 18, 2021
2012 review
Analytical strategies for the global quantification of intact proteins
[Review of ]. AMINO ACIDS, 43(3), 1109–1117.
author keywords: Proteomics; Intact proteins; Quantitation; Mass spectrometry
MeSH headings : Animals; Humans; Mass Spectrometry; Proteome / chemistry; Proteome / isolation & purification; Proteome / metabolism; Proteomics; Staining and Labeling; Two-Dimensional Difference Gel Electrophoresis
TL;DR:
Techniques contained in this review include the more traditional and widely employed methodology of differential gel electrophoresis and more recently developed mass spectrometry-based techniques including metabolic labeling, chemical labeling, and label-free methodologies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
Comprehensive Quantification of Monolignol-Pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry
Journal of Proteome Research, 11(6), 3390–3404.
Contributors: C. Shuford n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n , R. Shi n, R. Sederoff n, V. Chiang n, n
author keywords: quantification; isotope dilution mass spectrometry; selected reaction monitoring; filter-aided sample preparation; monolignol biosynthesis
MeSH headings : Amino Acid Sequence; Biosynthetic Pathways; Isotope Labeling; Molecular Sequence Data; Plant Proteins / chemistry; Plant Proteins / metabolism; Populus / enzymology; Proteome / chemistry; Proteome / metabolism; Reference Standards; Spectrometry, Mass, Electrospray Ionization / standards; Xylem / enzymology
TL;DR:
This represents the most comprehensive assay for quantification of proteins regulating monolignol biosynthesis and will lead to a better understanding of lignin formation at a systems level.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2012 journal article
Coordination-Driven Self-Assembly of Charged and Neutral Dendritic Tetrakis(ferrocenyl) Rhomboids
ORGANOMETALLICS, 31(20), 7241–7247.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
Evidence for Complex Molecular Architectures for Solvent-Extracted Lignins
ACS MACRO LETTERS, 1(5), 568–573.
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC-MS/MS based protein quantification and metabolic flux analysis
PLANTA, 236(3), 795–808.
Contributors: J. Wang n , C. Shuford n, Q. Li n, J. Song n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n
author keywords: Lignin; Syringyl lignin; Angiosperm; Kinetics; Subcellular localization
MeSH headings : Cloning, Molecular; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hydroxylation; Kinetics; Lignin / analysis; Lignin / biosynthesis; Mixed Function Oxygenases / metabolism; Plants, Genetically Modified; Populus / metabolism; Xylem / enzymology; Yeasts / metabolism
TL;DR:
The combination of genetic analysis, absolute protein quantitation-based enzyme kinetics, homologous CPR specificity, SNP characterization, and ER localization provides a more rigorous basis for a comprehensive systems understanding of 5-hydroxylation in lignin biosynthesis.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 article
High-resolution mass spectrometry
Maurer, H. H., & Muddiman, D. C. (2012, May). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 403, pp. 1201–1202.
MeSH headings : Animals; Biological Science Disciplines; Environmental Pollutants / analysis; Food Analysis; Humans; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Substance Abuse Detection
TL;DR:
An overview of the current use of high-resolution mass spectrometry in the life sciences is given, intended for all readers with an interest in application of HRMS in clinical and forensic toxicology, doping control, drug metabolism, food analysis, environmental sciences, and proteomics and related fields.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
In-Depth Analysis of the Magnaporthe oryzae Conidial Proteome
JOURNAL OF PROTEOME RESEARCH, 11(12), 5827–5835.
author keywords: proteomics; mass spectrometry; Magnaporthe oryzae; FASP; statistical tests; differential protein expression
MeSH headings : Cell Nucleus / chemistry; Chemical Fractionation / methods; Chromatography, Liquid / methods; Cytoplasm / chemistry; Fungal Proteins / analysis; Fungal Proteins / chemistry; Magnaporthe / chemistry; Mass Spectrometry / methods; Nanotechnology; Nuclear Proteins / analysis; Nuclear Proteins / chemistry; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spores, Fungal / chemistry; Time Factors
TL;DR:
The identification of 2912 conidial proteins at a 1% protein false discovery rate (FDR) is reported and the most extensive study performed on M. oryzae conidia to date is presented, suggesting the detected proteins build a representative portion of the predicted proteome.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
Peptide Production and Decay Rates Affect the Quantitative Accuracy of Protein Cleavage Isotope Dilution Mass Spectrometry (PC-IDMS)
Molecular & Cellular Proteomics, 11(9), 814–823.
MeSH headings : Indicator Dilution Techniques; Isotope Labeling; Mass Spectrometry; Peptide Biosynthesis; Peptides / analysis; Peptides / chemistry; Peptides / metabolism; Populus / enzymology; Populus / metabolism; Proteolysis; Xylem / enzymology
TL;DR:
It is concluded that the instability of peptides can often significantly bias the protein quantities measured in protein cleavage isotope dilution mass spectrometry-based assays and suggest peptide stability be made a priority when selecting peptides to use for quantification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2012 journal article
Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases
ACS Chemical Biology, 8(1), 200–208.
MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Chromatography, Liquid; Coenzyme A Ligases / chemistry; Coenzyme A Ligases / genetics; Crystallography, X-Ray; Genetic Variation; Models, Molecular; Substrate Specificity
TL;DR:
Results reveal that polyketide biosynthetic machinery might be more tolerant to non-natural substrates than previously established, and that mutant synthetases are valuable tools for probing the specificity of biosynthetics machinery.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2012 journal article
Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae
PLoS ONE, 7(8), e42868.
Ed(s): K. Nielsen
MeSH headings : Boronic Acids / pharmacology; Bortezomib; Cluster Analysis; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Germination / genetics; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Mutation; Nitrogen / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Polyubiquitin / genetics; Polyubiquitin / metabolism; Proteasome Inhibitors / pharmacology; Proteolysis / drug effects; Proteomics; Pyrazines / pharmacology; Ubiquitination
TL;DR:
This study suggests that ubiquitination of target proteins plays an important role in nutrient assimilation, development and pathogenicity of M. oryzae.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, Web Of Science
Added: August 6, 2018
2012 journal article
Systematic Comparison of Reverse Phase and Hydrophilic Interaction Liquid Chromatography Platforms for the Analysis of N-Linked Glycans
ANALYTICAL CHEMISTRY, 84(19), 8198–8206.
MeSH headings : Chromatography, Reverse-Phase; Hydrophobic and Hydrophilic Interactions; Mass Spectrometry; Polysaccharides / analysis
TL;DR:
The data presented show that separation of derivatized N-linked glycans by reverse phase chromatography significantly out-performs traditional separation of native or derivatization glycansBy hydrophilic interaction chromatography.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells
MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839.
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned / analysis; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Mice; Proteins / analysis; Secretory Pathway; Subcellular Fractions / chemistry
TL;DR:
Targeted proteomics of secretory pathway organelles is shown to be a powerful alternate approach for interrogating the cellular secretome and expected to be particularly valuable in two contexts highly relevant to hESC biology: obtaining a temporal snapshot of proteins secreted in response to a differentiation trigger, and identifying proteinssecreted by cells that are isolated from a heterogeneous population.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2012 journal article
The subcellular proteome of undifferentiated human embryonic stem cells
PROTEOMICS, 12(3), 421–430.
author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
TL;DR:
The results show that systematic subcellular proteome analysis is a valuable tool for studying hESC biology and shows that proteomic analysis of the endoplasmic reticulum and Golgi compartments is a powerful alternative approach to identify secreted proteins since these are synthesized in the ER and transit through the Golgi.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Absolute quantification of free glutathione and cysteine in aquatic insects using isotope dilution and selected reaction monitoring
Analytical and Bioanalytical Chemistry, 402(1), 357–366.
author keywords: Glutathione; Cysteine; Selected reaction monitoring; Absolute quantification; Isotope dilution
MeSH headings : Animals; Aquatic Organisms / chemistry; Carbon Isotopes / analysis; Chromatography, High Pressure Liquid / methods; Cysteine / analysis; Glutathione / analysis; Indicator Dilution Techniques; Insecta / chemistry; Nitrogen Isotopes / analysis
TL;DR:
A simple and robust isotope dilution mass spectrometry-based assay was developed for the determination of free cysteine and glutathione in aquatic insects and was demonstrated to have a quantitative dynamic range spanning nearly three orders of magnitude.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2011 review
Capitalizing on the hydrophobic bias of electrospray ionization through chemical modification in mass spectrometry-based proteomics
[Review of ]. EXPERT REVIEW OF PROTEOMICS, 8(3), 317–323.
author keywords: ALiPHAT; electrospray ionization; hydrophobic tagging; mass spectrometry; quantification
MeSH headings : Humans; Hydrophobic and Hydrophilic Interactions; Proteomics / methods; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
Improvements in targeted, mass spectrometry-based proteomic strategies are achieved by taking advantage of the mechanism of ESI through the use of hydrophobic chemical derivatization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.
MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
TL;DR:
The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Design, modeling, fabrication, and evaluation of the air amplifier for improved detection of biomolecules by electrospray ionization mass spectrometry
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 300(2-3), 99–107.
author keywords: Air amplifier; Electrospray ionization; Modeling; Detection limits
TL;DR:
A computer simulation that accurately portrays actual conditions and the results from these simulations are corroborated by the experimental data is developed, which can be used to predict outcomes from future designs resulting in a design process that is efficient in terms of financial cost and time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Evaluation of Normalization Methods on GeLC-MS/MS Label-Free Spectral Counting Data to Correct for Variation during Proteomic Workflows
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(12), 2199–2208.
author keywords: Label-free; Proteomics; Mass spectrometry; Total spectral count normalization; Normalized spectral abundance factor; Quantification; Spectral counting; Significance test; Fold-change; GeLC-MS/MS; nanoLC-MS; Orbitrap XL
TL;DR:
The correlation between SpCs of the same proteins across the different data sets was investigated and it was reported that TSpC normalization and NSAF normalization yielded almost ideal slopes of unity for normalized SpC versus average normalized SpCs, while NSP did not afford effective corrections of the unnormalized data.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Facile Self-Assembly of Supramolecular Hexakisferrocenyl Triangles via Coordination-Driven Self-Assembly and Their Electrochemical Behavior
ORGANOMETALLICS, 30(13), 3637–3642.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Foreword
International Journal of Mass Spectrometry, 300(2-3), 72–73.
Sources: Crossref, ORCID
Added: February 24, 2020
2011 journal article
Global optimization of the infrared matrix-assisted laser desorption electrospray ionization (IR MALDESI) source for mass spectrometry using statistical design of experiments
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(23), 3527–3536.
MeSH headings : Animals; Cattle; Cytochromes c / analysis; Cytochromes c / chemistry; Horses; Limit of Detection; Research Design; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
The use of statistical DOE is described to improve a few of the analytical figures of merit of the infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source for mass spectrometry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Hydrophobic Derivatization of N-linked Glycans for Increased Ion Abundance in Electrospray Ionization Mass Spectrometry
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(8), 1309–1317.
author keywords: Hydrophobic Derivatization; HILIC; nanoESI; N-Linked Glycans; Hydrazone Formation
MeSH headings : Blood Proteins / chemistry; Carbohydrate Sequence; Chromatography, Liquid; Glycoproteins / chemistry; Humans; Hydrazines / chemistry; Hydrophobic and Hydrophilic Interactions; Polysaccharides / chemistry; Small Molecule Libraries; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Mass Spectrometry
TL;DR:
The results of this study will direct the future synthesis of hydrophobic reagents for glycan analysis using the correlation between hydrophobicity and theoretical non-polar surface area calculation to facilitate the development of an optimum tag for gly can derivatization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Identification of alternative splice variants in Aspergillus flavus through comparison of multiple tandem MS search algorithms
BMC Genomics, 12.
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Improving Proteome Coverage on a LTQ-Orbitrap Using Design of Experiments
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(4), 773–783.
author keywords: LTQ-Orbitrap XL MS/MS instrument parameters; Design of experiments; Fractional-factorial design; Saccharomyces cerevisiae; Proteomics
MeSH headings : Peptide Fragments; Peptide Mapping; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods; Temperature
TL;DR:
The DOE approach represents a time- and cost-effective method for empirically optimizing MS-based proteomics workflows including sample preparation, LC conditions, and multiple instrument platforms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Increasing proteome coverage with offline RP HPLC coupled to online RP nanoLC-MS
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 879(9-10), 610–614.
author keywords: RP HPLC; Fractionation methods; Proteomics; Mass spectrometry
MeSH headings : Chromatography, High Pressure Liquid / methods; Chromatography, Reverse-Phase / methods; Mass Spectrometry / methods; Nanotechnology; Peptide Fragments / analysis; Peptide Fragments / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / metabolism
TL;DR:
The success of performing offline reversed phase high pressure liquid chromatography (HPLC) fractionation on a C18 2.0 mm×150 mm column at the peptide level with microliter per minute flow rates prior to online nano-flow reversed phase liquid Chromatography mass spectrometry (nanoLC-MS) using the well-studied fungus Saccharomyces cerevisiae is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
John B. Fenn special issue recognizing the science of Professor John B. Fenn foreword
International Journal of Mass Spectrometry, 300(2-3), 72–73.
Source: NC State University Libraries
Added: August 6, 2018
2011 article
John Bennett Fenn (1917-2010)
Muddiman, D. C. (2011, January 14). SCIENCE, Vol. 331, pp. 160–160.
MeSH headings : Chemistry / history; History, 20th Century; History, 21st Century; Nobel Prize; Spectrometry, Mass, Electrospray Ionization / history; United States
TL;DR:
The Nobel Prize in Chemistry did not define John Fenn, nor did it change him; he was the same man before and after, always showing a strong sense of humility.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Membrane protein complexes catalyze both 4-and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(52), 21253–21258.
MeSH headings : Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / metabolism; Chromatography, Liquid; Coumaric Acids; DNA Primers / genetics; Hydroxylation; Immunoprecipitation; Kinetics; Lignin / biosynthesis; Mass Spectrometry; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Microscopy, Confocal; Molecular Structure; Multiprotein Complexes / chemistry; Multiprotein Complexes / metabolism; Phenols; Phenylpropionates; Plasmids / genetics; Populus / metabolism; Propionates; Trans-Cinnamate 4-Monooxygenase / chemistry; Trans-Cinnamate 4-Monooxygenase / metabolism; Xylem / metabolism; Yeasts
TL;DR:
Affinity purification-quantitative mass spectrometry, bimolecular fluorescence complementation, chemical cross-linking, and reciprocal coimmunoprecipitation provide further evidence for functional involvement of membrane protein complexes in monolignol biosynthesis.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 chapter
N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry
In Methods in Molecular Biology (pp. 87–97).
author keywords: N-linked glycans; NanoLC mass spectrometry; Glycoproteins; Glycosylation; Glycan profiling; Hydrophilic interaction chromatography; Glycobiology; Glycan algorithms
MeSH headings : Acetylglucosamine / metabolism; Asparagine; Blood Proteins / chemistry; Blood Proteins / metabolism; Chromatography, Liquid / methods; Glycoproteins / metabolism; Glycoside Hydrolases / metabolism; Hydrophobic and Hydrophilic Interactions; Mannose / metabolism; Mass Spectrometry / methods; Nanotechnology / methods; Nitrogen / chemistry; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism; Polysaccharides / analysis; Polysaccharides / chemistry; Polysaccharides / isolation & purification; Polysaccharides / metabolism; Protein Denaturation; Solid Phase Extraction; Substrate Specificity; Tandem Mass Spectrometry
TL;DR:
Material, methods, and algorithms for the identification of both glycan composition and structure are summarized and potential problems and their resolutions are addressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: February 12, 2021
2011 journal article
One-year plasma N-linked glycome intra-individual and inter-individual variability in the chicken model of spontaneous ovarian adenocarcinoma
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 305(2-3), 79–86.
author keywords: N-linked glycans; HILIC; FTMS; Longitudinal sampling
TL;DR:
The initial interrogation of longitudinally-procured samples provides evidence that supports the value of this strategy in the search for glycomic biomarkers, and identifies 35 N-linked glycans identified in this study.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Performance Characteristics of a New Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer (TripleTOF 5600)
ANALYTICAL CHEMISTRY, 83(13), 5442–5446.
MeSH headings : Fungal Proteins / metabolism; Proteome; Saccharomyces cerevisiae / metabolism; Tandem Mass Spectrometry / instrumentation; Trypsin / metabolism
TL;DR:
The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Spectral Accuracy and Sulfur Counting Capabilities of the LTQ-FT-ICR and the LTQ-Orbitrap XL for Small Molecule Analysis
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(12), 2269–2275.
author keywords: Electrospray ionization; FT-ICR-MS; Orbitrap-MS; Forensic analysis; Textiles; Trace evidence
MeSH headings : Chi-Square Distribution; Coloring Agents / chemistry; Forensic Sciences; Fourier Analysis; Mass Spectrometry / methods; Polyesters / chemistry; Sensitivity and Specificity; Sulfur / analysis; Textiles / analysis
TL;DR:
DY42, a high-volume disperse dye for polyester, was used to compare the capabilities of the LTQ-Orbitrap XL and theLTQ-FT-ICR with respect to mass measurement accuracy (MMA), spectral accuracy, and sulfur counting.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2011 journal article
Stable-Isotope Labeled Hydrophobic Hydrazide Reagents for the Relative Quantification of N-Linked Glycans by Electrospray Ionization Mass Spectrometry
ANALYTICAL CHEMISTRY, 83(17), 6738–6745.
MeSH headings : Carbon Isotopes / chemistry; Glycopeptides / analysis; Glycosylation; Hydrazones / chemistry; Hydrophobic and Hydrophilic Interactions; Isotope Labeling; Polysaccharides / analysis; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
It is shown that these reagents are capable of quantifying changes in glycosylation in simple mixtures, and the analytical variability of the reagents in pooled plasma samples is shown to be less than ±30%.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Binding site on human immunoglobulin G for the affinity ligand HWRGWV
Journal of Molecular Recognition, 23(3), 271–282.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Detection of Alternative Splice Variants at the Proteome Level inAspergillus flavus
Journal of Proteome Research, 9(3), 1209–1217.
author keywords: mass spectrometry; proteomics; alternative splicing; database; Aspergillus flavus
MeSH headings : Alternative Splicing; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Computer Simulation; Cytochrome-B(5) Reductase / chemistry; Cytochrome-B(5) Reductase / genetics; Databases, Protein; Fungal Proteins / biosynthesis; Fungal Proteins / chemistry; Fungal Proteins / genetics; Isotope Labeling; Mass Spectrometry; Models, Genetic; Protein Isoforms / biosynthesis; Protein Isoforms / chemistry; Protein Isoforms / genetics; Proteomics; Pyruvate Carboxylase / chemistry; Pyruvate Carboxylase / genetics; RNA Splice Sites; Reproducibility of Results
TL;DR:
The introduction of an alternative splicing database helps identify more proteins and unveils more information about a proteome, and shows potential to improve genome annotation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: December 27, 2020
2010 journal article
Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics
ANALYTICAL CHEMISTRY, 82(20), 8696–8702.
MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
TL;DR:
A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Facile Self-Assembly of Dendritic Multiferrocenyl Hexagons and Their Electrochemistry
ORGANOMETALLICS, 29(22), 6137–6140.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Improving limits of detection for B-type natriuretic peptide using PC-IDMS: An application of the ALiPHAT strategy
ANALYST, 135(1), 36–41.
MeSH headings : Alkylating Agents / chemistry; Hydrophobic and Hydrophilic Interactions; Iodoacetamide / chemistry; Isotope Labeling; Limit of Detection; Natriuretic Peptide, Brain / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Trypsin / metabolism
TL;DR:
Evaluated alkylation reagents for their utility in quantifying B-type Natriuretic Peptide, a low abundance cardiac biomarker, by protein cleavage isotope dilution mass spectrometry found a approximately 3.5-fold decrease in the detection limit for the best performing hydrophobic reagent, 2-iodo-N-octylacetamide.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Increasing the hydrophobicity and electrospray response of glycans through derivatization with novel cationic hydrazides
CHEMICAL COMMUNICATIONS, 46(2), 237–239.
TL;DR:
Novel tags are used to increase the hydrophobicity of glycans and impart a permanent charge yielding as great as a approximately 5-fold increase in electrospray response from both a standard and complex mixture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 article
Interplay of Permanent Charge and Hydrophobicity in the Electrospray Ionization of Glycans
Walker, S. H., Papas, B. N., Comins, D. L., & Muddiman, D. C. (2010, August 1). ANALYTICAL CHEMISTRY, Vol. 82, pp. 6636–6642.
TL;DR:
The effectiveness of the incorporation of a permanent charge is studied and determined to hamper glycan analysis and a procedure for glycan hydrazone formation is optimized and outlined where a large number of variables were simultaneously analyzed using a fractional factorial design (FFD).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(2), 737–749.
Contributors: A. Hawkridge n, R. Wysocky n, J. Petitte n , K. Anderson n , P. Mozdziak n , O. Fletcher n, J. Horowitz n, n
author keywords: Genomics/proteomics; Mass spectrometry/ICP-MS; Biological samples; Bioanalytical methods; Clinical/biomedical analysis
MeSH headings : Adenocarcinoma / metabolism; Adenocarcinoma / pathology; Animals; Chickens; Female; Humans; Mass Spectrometry / methods; Ovarian Neoplasms / metabolism; Ovarian Neoplasms / pathology; Plasma / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods
TL;DR:
An experimental strategy for biomarker discovery is reported herein that combines the chicken model of EOC, longitudinal plasma sample collection with matched tissues, advanced mass spectrometry-based proteomics, and concepts derived from the index of individuality in an effort to identify diagnostic protein biomarkers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 article
Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma (vol 398, pg 737, 2010)
Hawkridge, A. M., Wysocky, R. B., Petitte, J. N., Anderson, K. E., Mozdziak, P. E., Fletcher, O. J., … Muddiman, D. C. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1835–1835.
Contributors: A. Hawkridge n, R. Wysocky n, J. Petitte n , K. Anderson n , P. Mozdziak n , O. Fletcher n, J. Horowitz n, n
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 377–389.
author keywords: Secretome; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Thermophile; Sample preparation
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Extracellular Space / metabolism; Gram-Positive Bacteria / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature
TL;DR:
In an effort to define the extracellular proteome of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, several techniques were considered regarding sample processing to achieve the most in-depth analysis of secreted proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 article
Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2 (vol 398, pg 377, 2010)
Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1837–1837.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 391–404.
author keywords: Thermotoga maritima; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Co-cultivation; Intracellular proteome; Spectral counting
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chemotaxis; Chromatography, Liquid; Coculture Techniques; Electrophoresis, Polyacrylamide Gel; Fourier Analysis; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Intracellular Space / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis / statistics & numerical data; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR:
Altering in growth conditions of these bacterial thermophilic microorganisms offer a glimpse into the intricacy of microbial behavior and interaction as well as insight into microbial interaction and behavior.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 article
Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting (vol 398, pg 391, 2010)
Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1839–1839.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Quantitative top-down proteomics of SILAC labeled human embryonic stem cells
Journal of the American Society for Mass Spectrometry, 21(6), 879–889.
MeSH headings : Amino Acid Sequence; Animals; Arginine / chemistry; Arginine / metabolism; Carbon Isotopes; Cell Line; Embryonic Stem Cells / chemistry; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Lysine / chemistry; Lysine / metabolism; Mass Spectrometry / methods; Mice; Proteome / analysis; Proteomics / methods
TL;DR:
This study has confidently identified 11 proteins by accurate intact mass, MS/MS, and amino acid counting facilitated by SILAC labeling, which to the best of the authors' knowledge represents the first top-down analysis of hESCs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2010 journal article
Self-Assembly of Dendritic Tris(crown ether) Hexagons and Their Complexation with Dibenzylammonium Cations
JOURNAL OF ORGANIC CHEMISTRY, 75(21), 7373–7380.
MeSH headings : Benzylammonium Compounds / chemistry; Crown Ethers / chemistry; Dendrimers / chemistry; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Conformation
TL;DR:
Combining 120° crown ether-containing diplatinum(II) acceptors with 120° dendritic dipyridyl donors in a 1:1 ratio allows for the formation of a new family of dendrite triple crown ether derivatives with a hexagonal cavity in quantitative yields.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Study of the ionization mechanism in hybrid laser based desorption techniques
ANALYST, 135(5), 880–882.
TL;DR:
The purpose of these experiments is to test the hypothesis that the primary ionization pathway in MALDESI is through interaction of the laser-desorbed neutral species with the intersecting electrospray ionization beam.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
The effects of abundant plasma protein depletion on global glycan profiling using NanoLC FT-ICR mass spectrometry
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 396(4), 1473–1479.
author keywords: HILIC; NanoLCFT-ICRMS; Glycan profiling; N-linked glycans; Protein depletion
MeSH headings : Blood Proteins / chemistry; Blood Proteins / standards; Chromatography, Liquid; Nanotechnology; Polysaccharides / chemistry; Polysaccharides / standards; Proteomics / instrumentation; Proteomics / methods; Reproducibility of Results; Spectroscopy, Fourier Transform Infrared
TL;DR:
Protein depletion was found to marginally effect the abundance of high mannose, hybrid, and large highly sialylated complex species and small fucosylated non-sialylation complex glycans were found to decrease in abundance in the depleted samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2010 journal article
Utilizing Spectral Counting To Quantitatively Characterize Tandem Removal of Abundant Proteins (TRAP) in Human Plasma
ANALYTICAL CHEMISTRY, 82(24), 10179–10185.
MeSH headings : Biomarkers / analysis; Blood Proteins / analysis; Blood Proteins / isolation & purification; Humans
TL;DR:
The data demonstrate that this method, tandem removal of abundant proteins or TRAP, provides superior removal efficiency compared to traditional applications and improves the depth of proteome coverage in plasma.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Absolute Quantification of C-Reactive Protein in Human Plasma Derived from Patients with Epithelial Ovarian Cancer Utilizing Protein Cleavage Isotope Dilution Mass Spectrometry
JOURNAL OF PROTEOME RESEARCH, 8(2), 1085–1090.
author keywords: absolute quantification; C-reactive protein; ovarian cancer; single reaction monitoring
MeSH headings : Amino Acid Sequence; C-Reactive Protein / chemistry; C-Reactive Protein / metabolism; Chromatography, Liquid / methods; Female; Humans; Isotope Labeling / methods; Mass Spectrometry / methods; Molecular Sequence Data; Ovarian Neoplasms / blood; Ovarian Neoplasms / chemistry; Peptides / chemistry; Peptides / genetics; Peptides / metabolism; Reproducibility of Results
TL;DR:
Interestingly, the PC-IDMS method provided concentration values that were approximately 10x the concentration reported by the ELISA method, which demonstrated that the method of detection is an important consideration when determining reference ranges of a particular analyte.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Atmospheric pressure infrared (10.6 mu m) laser desorption electrospray ionization (IR-LDESI) coupled to a LTQ Fourier transform ion cyclotron resonance mass spectrometer
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 23(13), 1989–1992.
MeSH headings : Animals; Cattle; Molecular Weight; Proteins / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods
TL;DR:
The implementation of a CO(2) laser emitting infrared laser irradiation at 10.6 microm onto the versatile atmospheric pressure ionization platform is presented and Multiply charged proteins ranging in molecular mass from 8.6 to 17 kDa were detected from liquid-state samples without the addition of matrix.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Binding site on human immunoglobulin G for the affinity ligand HWRGWV
Journal of Molecular Recognition, n/a-n/a.
author keywords: hexamer ligand; immunoglobulin G; glycosylation; mass spectrometry; computational docking; binding site
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Endopeptidases / metabolism; Humans; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / chemistry; Immunoglobulin G / metabolism; Ligands; Models, Molecular; Molecular Sequence Data; Nerve Tissue Proteins / metabolism; Oligopeptides / chemistry; Oligopeptides / genetics; Oligopeptides / metabolism; Pepsin A / metabolism; Protein Binding; Protein Structure, Tertiary; Sequence Alignment; Staphylococcal Protein A / metabolism
TL;DR:
The results support the fact that a peptide as small as a hexamer can have specific interactions with large proteins such as hIgG and the HWRGWV ligand.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: February 24, 2020
2009 journal article
Characterization of Charge Separation in the Array of Micromachined UltraSonic Electrospray (AMUSE) Ion Source for Mass Spectrometry
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20(9), 1684–1687.
MeSH headings : Equipment Design; Equipment Failure Analysis; Radiation Dosage; Radiometry; Sonication; Spectrometry, Mass, Electrospray Ionization / instrumentation; Static Electricity
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Coupling of a vented column with splitless nanoRPLC-ESI-MS for the improved separation and detection of brain natriuretic peptide-32 and its proteolytic peptides
Journal of Chromatography B, 877(10), 948–954.
author keywords: Vented column; BNP-32; Splitless nanoLC; Tryptic digestion
MeSH headings : Amino Acid Sequence; Brain Chemistry; Chromatography, Liquid / methods; Molecular Sequence Data; Natriuretic Peptide, Brain / chemistry; Natriuretic Peptide, Brain / isolation & purification; Natriuretic Peptide, Brain / pharmacokinetics; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
It is demonstrated that the use of free cysteine as an alkylation quenching agent and a secondary digestion within the digestion scheme can provide targeted tryptic peptides with increased abundances, which will serve to further augment the detection of BNP-32 by LC-MS.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2009 journal article
Development of a Robust and High Throughput Method for Profiling N-Linked Glycans Derived from Plasma Glycoproteins by NanoLC-FTICR Mass Spectrometry
JOURNAL OF PROTEOME RESEARCH, 8(7), 3764–3770.
MeSH headings : Biomarkers, Tumor / metabolism; Blood Proteins / chemistry; Chromatography, Liquid / methods; Detergents / pharmacology; Glycoproteins / chemistry; Humans; Mass Spectrometry / methods; Microwaves; Peptides / chemistry; Polysaccharides / chemistry; Proteome; Proteomics / methods; Spectroscopy, Fourier Transform Infrared / methods; Time Factors
TL;DR:
A step-by-step protocol describing a method for N-linked glycan profiling of plasma glycoproteins by nanoflow liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Development of a nanoLC LTQ Orbitrap Mass Spectrometric Method for Profiling Glycans Derived from Plasma from Healthy, Benign Tumor Control, and Epithelial Ovarian Cancer Patients
ANALYTICAL CHEMISTRY, 81(3), 1130–1136.
Contributors: M. Bereman n, T. Williams n & n
MeSH headings : Biomarkers, Tumor / blood; Biomarkers, Tumor / chemistry; Carcinoma / diagnosis; Chromatography, Liquid / methods; Female; Genital Neoplasms, Female / chemistry; Genital Neoplasms, Female / diagnosis; Glycoproteins / blood; Glycoproteins / chemistry; Humans; Hydrophobic and Hydrophilic Interactions; Lectins / blood; Lectins / chemistry; Male; Ovarian Neoplasms / diagnosis; Polysaccharides / blood; Polysaccharides / chemistry; ROC Curve; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A split-less nano-flow liquid chromatography mass spectrometric analysis of glycans chemically cleaved from glycoproteins in plasma shows similar column efficiencies and excellent retention time reproducibility without an internal standard to correct for retention time shift.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Evaluation of the ALiPHAT Method for PC-IDMS and Correlation of Limits-of-Detection with Nonpolar Surface Area
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20(11), 2006–2012.
MeSH headings : Alkylation; Amino Acid Sequence; Calibration; Carbon Isotopes / chemistry; Chromatography, High Pressure Liquid / methods; Humans; Hydrophobic and Hydrophilic Interactions; Isotope Labeling / methods; Laminin / analysis; Laminin / chemistry; Male; Mass Spectrometry / methods; Nitrogen Isotopes / chemistry; Oligopeptides / analysis; Oligopeptides / chemistry; Prostate-Specific Antigen / chemistry; Reference Standards; Trypsin / analysis; Trypsin / chemistry
TL;DR:
While an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophOBicity, the number of decreases in LOD was observed, the authors observed a decrease.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Generation of Multiply Charged Peptides and Proteins by Radio Frequency Acoustic Desorption and Ionization for Mass Spectrometric Detection
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20(4), 597–600.
MeSH headings : Electrodes; Equipment Design; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Natriuretic Peptide, Brain / chemistry; Peptides / chemistry; Proteins / chemistry; Quartz / chemistry; Ubiquitin / chemistry
TL;DR:
The design and implementation of a radio frequency acoustic desorption ionization (RADIO) source has been demonstrated and intact and dissociated species observed during MS and MS/MS experiments, respectively are confirmed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Intact and Top-Down Characterization of Biomolecules and Direct Analysis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Coupled to FT-ICR, Mass Spectrometry
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20(4), 667–673.
MeSH headings : Amino Acid Sequence; Animals; Carbohydrates / chemistry; Cattle; Chickens; Egg Yolk / chemistry; Horses; Lipids / chemistry; Milk / chemistry; Molecular Sequence Data; Mucins / chemistry; Mucins / metabolism; Myoglobin / chemistry; Polysaccharides / chemistry; Polysaccharides / metabolism; Proteins / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
TL;DR:
Three of the four classes of biological macromolecules have been ionized and detected using MALDESI with minimal sample preparation and Sequencing of O-linked glycans, cleaved from mucin using reductive β-elimination chemistry, is also demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 article
Mass Spectrometry-Based Biomarker Discovery: Toward a Global Proteome Index of Individuality
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, Vol. 2, pp. 265–277.
author keywords: biological variability; analytical variability; longitudinal sampling
MeSH headings : Animals; Biomarkers / analysis; Humans; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Proteome / analysis; Proteomics / instrumentation; Proteomics / methods
TL;DR:
The purpose of this review is to discuss the analytical methods and strategies that have been developed for mass spectrometry-based biomarker-discovery platforms and to place them in the context of the many challenges and opportunities yet to be addressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Proteomics Characterization of Cell Membrane Blebs in Human Retinal Pigment Epithelium Cells
MOLECULAR & CELLULAR PROTEOMICS, 8(10), 2201–2211.
MeSH headings : Aged; Animals; Antioxidants / pharmacology; Basigin / analysis; Cell Line; Cell Membrane / chemistry; Cell Membrane / drug effects; Cell Membrane / ultrastructure; Cell Surface Extensions / chemistry; Cell Surface Extensions / drug effects; Cell Surface Extensions / ultrastructure; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Epithelial Cells / chemistry; Epithelial Cells / cytology; Epithelial Cells / drug effects; Extracellular Matrix / chemistry; Extracellular Matrix / metabolism; Eye Proteins / analysis; Humans; Hydroquinones / pharmacology; Macular Degeneration / pathology; Matrix Metalloproteinase 14 / analysis; Matrix Metalloproteinase 2 / analysis; Molecular Sequence Data; Proteomics / methods; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Retinal Pigment Epithelium / chemistry; Retinal Pigment Epithelium / cytology; Smoke; Smoking / adverse effects; Tandem Mass Spectrometry; Tobacco / chemistry
TL;DR:
Basigin and matrix metalloproteinase-14, key proteins involved in extracellular matrix remodeling, were identified in RPE blebs and shown to be more prevalent in AMD patients.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Species-dependent hepatic metabolism of immunosuppressive agent tacrolimus (FK-506)
Xenobiotica, 39(10), 757–765.
author keywords: Tacrolimus; species differences; liver microsomes; cytochrome P-450; secondary ion mass spectrometry (SIMS); matrix-assisted laser desorption ionization (MALDI)
MeSH headings : Animals; Chromatography, High Pressure Liquid; Cricetinae; Cytochrome P-450 Enzyme System / metabolism; Dogs; Guinea Pigs; Humans; Immunosuppressive Agents / chemistry; Immunosuppressive Agents / metabolism; Kinetics; Microsomes, Liver / enzymology; Papio; Rabbits; Rats; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tacrolimus / chemistry; Tacrolimus / metabolism
TL;DR:
From the current in-vitro metabolism studies, the pattern of tacroliums metabolism in baboons closely resembled that in humans and thus it is ideal for studying tacrolimus metabolism-related work of clinical relevance.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2009 journal article
Synthesis of Six-Component Metallodendrimers via [3+3] Coordination-Driven Self-Assembly
JOURNAL OF ORGANIC CHEMISTRY, 74(9), 3524–3527.
MeSH headings : Dendrimers / chemical synthesis; Dendrimers / chemistry; Models, Molecular; Molecular Conformation; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry
TL;DR:
A new class of 120 degrees dendritic di-Pt(II) acceptor subunits has been designed and synthesized, from which six-component hexagonal metallodendrimers were easily formed with 120 degreesdendritic dipyridine donors via [3 + 3] coordination-driven self-assembly.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Tandem Mass Spectrometry of Thiolate-Protected Au Nanoparticles NaxAu25(SC2H4Ph)(18-y)(S(C2H4O)(5)CH3)(y)
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131(38), 13844–13851.
Contributors: C. Fields Zinna n, J. Sampson n, M. Crowe n, J. Tracy n , J. Parker n, A. Deney n, n , R. Murray n
MeSH headings : Crystallography, X-Ray; Gold / chemistry; Metal Nanoparticles / chemistry; Sulfhydryl Compounds / chemistry; Tandem Mass Spectrometry
TL;DR:
The first collision-induced dissociation tandem mass spectrometry (CID MS/MS) of a thiolate-protected Au nanoparticle that has a crystallographically determined structure is reported, and previously unreported results show that even soft ionization sources can result in Au nanoparticles fragmentation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2009 journal article
Utilizing Artificial Neural Networks in MATLAB to Achieve Parts-Per-Billion Mass Measurement Accuracy with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20(7), 1303–1310.
MeSH headings : Fourier Analysis; Linear Models; Mass Spectrometry / methods; Models, Theoretical; Neural Networks, Computer; Reproducibility of Results; Sensitivity and Specificity
TL;DR:
Artificial neural network calibration in conjunction with automatic gain control (AGC) is utilized in these experiments to formally account for the differences in total ion population in the ICR cell between the external calibration spectra and experimental spectra.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
A new family of multiferrocene complexes with enhanced control of structure and stoichiometry via coordination-driven self-assembly and their electrochemistry
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(3), 839-+.
MeSH headings : Electrochemistry; Ferrous Compounds / chemistry; Metallocenes; Molecular Structure; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry
TL;DR:
The design and synthesis of a new family of multiferrocene complexes with enhanced control of structure and stoichimetry via coordination-driven self-assembly is described, providing an enhanced understanding of the influence of structural factors on the electrochemistry of multifunctional electroactive supramolecular architectures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Ambient aerodynamic ionization source for remote analyte sampling and mass spectrometric analysis
ANALYTICAL CHEMISTRY, 80(13), 5266–5271.
MeSH headings : Acetaminophen / antagonists & inhibitors; Acetaminophen / chemistry; Caffeine / analysis; Caffeine / chemistry; Natriuretic Peptide, Brain / analysis; Natriuretic Peptide, Brain / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ubiquitin / analysis; Ubiquitin / chemistry
TL;DR:
This novel aerodynamic device, herein termed remote analyte sampling, transport, and ionization relay (RASTIR) was used to remotely sample neutral species in the ambient and entrain them into an electrospray plume where they were subsequently ionized and detected using a linear ion trap Fourier transform mass spectrometer.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Calibration laws based on multiple linear regression applied to matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
JOURNAL OF MASS SPECTROMETRY, 43(12), 1659–1663.
Contributors: D. Williams Jr., M. Chadwick n, T. Williams n & n
author keywords: multiple linear regression; polypropylene glycol; calibration; matrix-assisted laser desorption/ionization (MALDI); Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS); space-charge effects
MeSH headings : Calibration; Cyclotrons; Fourier Analysis; Linear Models; Mass Spectrometry / methods; Multivariate Analysis; Peptide Mapping / methods; Peptide Mapping / statistics & numerical data; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
An evaluation of external mass calibration laws applied to MALDI-FT-ICR-MS is performed to achieve higher mass measurement accuracy (MMA) in Fourier transform ion cyclotron resonance mass spectrometry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
1. No Poverty
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Construction of a versatile high precision ambient Ionization source for direct analysis and imaging
Journal of the American Society for Mass Spectrometry, 19(10), 1527–1534.
MeSH headings : Bradykinin / chemistry; Cations / chemistry; Humans; Lasers; Melitten / chemistry; Muramidase / chemistry; Myoglobin / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ubiquitin / chemistry
TL;DR:
The design and construction of a high precision ambient ionization source matrix-assisted laser desorption electrospray ionization (MALDESI) and the computer controlled high precision motion control system and high repetition rate Explorer laser are demonstrated during MALDESI-FT-ICR analysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2008 journal article
Coordination-driven face-directed self-assembly of trigonal prisms. Face-based conformational chirality
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(24), 7620–7628.
MeSH headings : Magnetic Resonance Spectroscopy; Molecular Conformation; Pyridines / chemistry; Spectrometry, Mass, Electrospray Ionization; Stereoisomerism; Temperature
TL;DR:
NMR data prove that the two edges of the pyridine rings in the triflate salts of 3a-3d are distinct and molecular mechanics results suggest that in the smallest prism 3d collective effects dominate and the all-P and all-M conformers are strongly favored.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 review
Coordination-driven self-assembly of cavity-cored multiple crown ether derivatives and poly[2]pseudorotaxanes
[Review of ]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(15), 5320–5334.
MeSH headings : Crown Ethers / chemical synthesis; Crown Ethers / chemistry; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Polymers / chemistry; Rotaxanes / chemistry; Spectrometry, Mass, Electrospray Ionization; Thermodynamics
TL;DR:
The synthesis of a new 120 degree diplatinum(II) acceptor unit and the self-assembly of a series of two-dimensional metallacyclic polypseudorotaxanes that utilize both metal-ligand and crown ether-dialkylammonium noncovalent interactions are described, demonstrating the utility and scope of this orthogonal self- assembly technique.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Development and characterization of an ionization technique for analysis of biological macromolecules: Liquid matrix-assisted laser desorption electrospray ionization
ANALYTICAL CHEMISTRY, 80(17), 6773–6778.
MeSH headings : Organic Chemicals / chemistry; Peptides / analysis; Proteins / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Surface Properties
TL;DR:
An increase in ion abundance and a concurrent decrease in surface tension with an increase in the applied potential are demonstrated and a singly charged radical cation of an electrochemically active species indicating oxidation occurs for analytes and therefore water is observed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Effect of Plasma Protein Depletion on BNP-32 Recovery
Clinical Chemistry, 54(5), 933–934.
Sources: NC State University Libraries, ORCID
Added: March 5, 2021
2008 personal communication
Effect of plasma protein depletion on BNP-32 recovery
Hawkridge, A. M., Muddiman, D. C., Helmlein, D. M., Cataliotti, A., & Burnett, J. C., Jr. (2008, May).
MeSH headings : Blood Proteins / metabolism; Humans; Iodine Radioisotopes; Natriuretic Peptide, Brain / blood; Protein Binding
TL;DR:
A targeted biomarker discovery platform for characterizing the circulating forms of B-type natriuretic peptide that includes protein depletion strategies, immunoprecipitation, gel electrophoresis, isotope dilution (absolute quantification), and nanoflow liquid chromatography coupled to high-performance hybrid Fourier transform mass spectrometry is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Human Proteinpedia enables sharing of human protein data
Nature Biotechnology, 26(2), 164–167.
MeSH headings : Database Management Systems; Databases, Protein; Gene Expression Profiling / methods; Information Storage and Retrieval / methods; Internationality; Internet; Magnetic Resonance Spectroscopy / methods; Peptide Mapping / methods; Proteome / chemistry; Proteome / classification; Proteome / metabolism; Proteomics / methods; Software; User-Computer Interface
TL;DR:
MMCD is a practical tool for expediting the time-consuming steps of identifying and researching small molecules and is compatible with both NMR and MS data and facilitates high-throughput metabolomics investigations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: February 24, 2020
2008 personal communication
Human Proteinpedia enables sharing of human protein data
Source: NC State University Libraries
Added: August 6, 2018
2008 journal article
Investigations with O-linked protein glycosylations by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
JOURNAL OF MASS SPECTROMETRY, 43(9), 1215–1223.
Contributors: T. Williams n , D. Saggese n, K. Toups n, J. Frahm n, H. An *, B. Li*, C. Lebrilla *, n
author keywords: Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS); matrix-assisted laser desorption/ionization (MALDI); mucin; O-linked glycans; protein glycosylations
MeSH headings : Angiotensins / chemistry; Animals; Glycosylation; Mucins / chemistry; Oligosaccharides / chemistry; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Swine; Tandem Mass Spectrometry / methods
TL;DR:
A detailed examination of sample preparation and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT-ICR-MS) analysis of O-linked glycans globally cleaved from mucin glycoproteins is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Mass measurement accuracy comparisons between a double-focusing magnetic sector and a time-of-flight mass analyzer
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 22(10), 1563–1566.
MeSH headings : Equipment Design; Equipment Failure Analysis; Magnetics / instrumentation; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
TL;DR:
A direct comparison of the mass measurement accuracies (MMAs) obtained on different mass spectrometry instrument types; a magnetic sector as the 'gold standard' and an electrospray ionization time-of-flight (ESI-TOF) instrument.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Studying O-linked protein glycosylations in human plasma
JOURNAL OF PROTEOME RESEARCH, 7(6), 2562–2568.
Contributors: T. Williams n , D. Saggese n & n
author keywords: protein O-glycosylations; plasma; epithelial ovarian cancer (EOC); matrix-assisted laser desorption/iozation (MALDI); Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR-MS)
MeSH headings : Blood Proteins / chemistry; Blood Proteins / metabolism; Borohydrides / chemistry; Dialysis / methods; Female; Glycosylation; Hexosamines / analysis; Hexoses / analysis; Hexuronic Acids / analysis; Humans; Middle Aged; Ovarian Neoplasms / blood; Polysaccharides / analysis; Polysaccharides / chemistry; Polysaccharides / isolation & purification; Pronase / metabolism; Solid Phase Extraction / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
The protocol here identifies O-linked carbohydrate patterns in EOC plasma glycoproteins through chemical cleavage and purification of these glycans through chemical Cleavage and Purification ofThese glycans are identified through carbohydrate pattern analysis with MALDI-FT-ICR-MS.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Synthesis, Characterization, and Application of Iodoacetamide Derivatives Utilized for the ALiPHAT Strategy
Journal of the American Chemical Society, 130(7), 2122–2123.
MeSH headings : Alkylation; Amino Acid Sequence; Cysteine / chemistry; Factor VIIIa / antagonists & inhibitors; Hydrophobic and Hydrophilic Interactions; Iodoacetamide / analogs & derivatives; Iodoacetamide / chemical synthesis; Iodoacetamide / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
Hydrophobic tags, derived from iodoacetamide, were synthesized, characterized, and utilized to alkylate peptide E-76, a known inhibitor of coagulation factor VIIIa, and two other cysteine containing peptides to demonstrate an increase in electrospray response.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: February 24, 2020
2008 journal article
Synthesis, characterization, and application of lodoacetamide derivatives utilized for the ALiPHAT strategy
Journal of the American Chemical Society, 130(7), 2122-.
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2008 journal article
Temperature-dependent regulation of proteins in Aspergillus flavus: Whole organism stable isotope labeling by amino acids
JOURNAL OF PROTEOME RESEARCH, 7(7), 2973–2979.
author keywords: SILAC; mass spectrometry; proteomics; Aspergillus flavus; aflatoxin
MeSH headings : Aflatoxins / biosynthesis; Amino Acids / metabolism; Arginine / metabolism; Aspergillus flavus / metabolism; Carbon Isotopes; Chromatography, Liquid; Culture Media; Fungal Proteins / biosynthesis; Fungal Proteins / genetics; Gene Expression Regulation, Fungal; Isotope Labeling; Oligonucleotide Array Sequence Analysis; Tandem Mass Spectrometry; Temperature
TL;DR:
The utility of this procedure in quantifying changes in protein expression in response to environmental stimuli is shown, and the first reported labeling of a multicellular free-living prototroph using the SILAC procedure to compare (13)C(6)-arginine-labeled samples to (12)C (6-arginines-labeling samples for quantitative proteomics is reported.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2008 journal article
Top-down identification and quantification of stable isotope labeled proteins from Aspergillus flavus using online nano-flow reversed-phase liquid chromatography coupled to a LTQ-FTICR mass spectrometer
ANALYTICAL CHEMISTRY, 80(13), 4994–5001.
MeSH headings : Arginine / chemistry; Arginine / metabolism; Aspergillus flavus / chemistry; Aspergillus flavus / metabolism; Carbon Radioisotopes / chemistry; Chromatography, Liquid / methods; Fourier Analysis; Fungal Proteins / analysis; Fungal Proteins / chemistry; Fungal Proteins / metabolism; Isotope Labeling; Tandem Mass Spectrometry / methods
TL;DR:
The qualitative and quantitative top-down characterization of proteins derived from SILAC-labeled Aspergillus flavus is described using nanoflow reversed-phase liquid chromatography directly coupled to a linear ion trap Fourier transform ion cyclotron resonance mass spectrometer (nLC-LTQ-FTICR-MS).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
A highly efficient approach to the self-assembly of hexagonal cavity-cored tris[2]pseudorotaxanes from several components via multiple noncovalent interactions
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 129(46), 14187-+.
MeSH headings : Crystallography, X-Ray; Magnetic Resonance Spectroscopy; Models, Chemical; Organoplatinum Compounds / chemistry; Rotaxanes / chemical synthesis; Spectrometry, Mass, Electrospray Ionization
TL;DR:
This methodology demonstrates the power of orthogonal self-assembly and may be expanded beyond the use of two recognition motifs in hopes of mimicking the complex multifunctionality of biological systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
A method for automatically interpreting mass spectra of 180-labeled isotopic clusters
MOLECULAR & CELLULAR PROTEOMICS, 6(2), 305–318.
MeSH headings : Algorithms; Chromatography, High Pressure Liquid; Cluster Analysis; Computational Biology; Mass Spectrometry / methods; Oxygen Isotopes / metabolism; Peptide Mapping; Proteome / metabolism; Proteomics / methods; Regression Analysis
TL;DR:
An algorithm entitled regression analysis applied to mass spectrometry (RAAMS) is developed that automatically, rapidly, and confidently interprets spectra of 18O-labeled peptides without requiring chemical composition information derived from product ion spectra.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Achieving augmented limits of detection for peptides with hydrophobic alkyl tags
ANALYTICAL CHEMISTRY, 79(11), 3989–3995.
MeSH headings : Acetates / chemistry; Alkylation; Amides / chemical synthesis; Amides / chemistry; Amino Acid Sequence; Chlorides / chemistry; Hydrophobic and Hydrophilic Interactions; Iodine / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
TL;DR:
The results demonstrate the feasibility of the octylcarboxyamidomethyl modification to improve limits of detection for cysteine-containing peptides with hydrophobic alkyl tags.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Carbohydrate analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
ANALYTICAL CHEMISTRY, 79(22), 8812–8815.
Contributors: M. Bereman n, T. Williams n & n
MeSH headings : Carbohydrates / analysis; Cyclotrons; Fourier Analysis; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
G glycans from mucin are identified by using the high mass measurement accuracy and tandem MS capabilities afforded by the hybrid FT-ICR-MS platform.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Details of the Structure Determination of the Sulfated Steroids PSDS and PADS: New Components of the Sea Lamprey (Petromyzonmarinus) Migratory Pheromone
The Journal of Organic Chemistry, 72(20), 7544–7550.
MeSH headings : Animals; Cholestanes / chemistry; Molecular Conformation; Nuclear Magnetic Resonance, Biomolecular; Petromyzon / metabolism; Pheromones / chemistry; Pyrrolidinones / chemistry
TL;DR:
Details of the structure determination of these steroidal bis-sulfates of the migratory pheromone used by sea lamprey to guide adults to spawning grounds are described and logic underlying the iterative analyses used is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Crossref, ORCID
Added: January 18, 2021
2007 journal article
Details of the structure determination of the sulfated steroids PSDS and PADS: New components of the sea lamprey (Petromyzon marinus) migratory pheromone
Journal of Organic Chemistry, 72(20), 7544–7550.
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2007 journal article
Detection of attomole amounts of analyte by desorption electrospray ionization mass spectrometry (DESI-MS) determined using fluorescence spectroscopy
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 18(6), 1093–1096.
MeSH headings : Microchemistry / methods; Nanotechnology / methods; Rhodamines / chemistry; Spectrometry, Fluorescence / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
The use of fluorescence spectroscopy is reported to investigate the amount of material removed from a PTFE surface and detected during desorption electrospray ionization (DESI) mass spectrometry measurements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Differential protein expression in male and female human lumbar cerebrospinal fluid using iTRAQ reagents after abundant protein depletion
PROTEOMICS, 7(20), 3726–3734.
author keywords: ceruloplasmin; ferroxidase; gender; low-abundance protein; mass spectrometry
MeSH headings : Adult; Aged; Biomarkers / analysis; Biomarkers / metabolism; Cerebrospinal Fluid Proteins / analysis; Cerebrospinal Fluid Proteins / metabolism; Female; Humans; Indicators and Reagents; Lumbosacral Region; Male; Middle Aged; Proteome / analysis; Proteome / metabolism
TL;DR:
This study examined the differential expression of proteins in pooled male and female CSF utilizing isobaric tags for relative and absolute quantification (iTRAQ™) reagents after the depletion of six high abundant proteins using a multiple affinity removal system (MARS).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Direct characterization of intact polypeptides by matrix-assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(7), 1150–1154.
MeSH headings : Cyclotrons; Microchemistry / methods; Molecular Weight; Peptides / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
TL;DR:
The ability of MALDESI-QFT-ICR MS to directly analyze and provide high mass measurement accuracy (approximately 1 part-per-million) of a polypeptide using internal calibration is demonstrated and the potential of this system for the top-down characterization of multiply charged polyPEptide cations is shown.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Dual electrospray ion source for electron-transfer dissociation on a hybrid linear ion trap-orbitrap mass spectrometer
ANALYTICAL CHEMISTRY, 79(20), 7916–7919.
MeSH headings : Electrons; Ions; Molecular Structure; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The stability of the ETD anion population is demonstrated and the ability to identify several c- and z-type product ions from multiply protonated peptide cations is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Effect of matrix crystal structure on ion abundance of carbohydrates by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(5), 807–811.
Contributors: T. Williams n , D. Saggese n, R. Wilcox n, J. Martin n & n
MeSH headings : Acetylglucosamine / chemistry; Crystallization; Cyclotrons; Ions; N-Acetylneuraminic Acid / chemistry; Powder Diffraction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared
TL;DR:
Sample preparation techniques for carbohydrate analysis using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are explored, with particular emphasis on analyte/matrix co-crystallization procedures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Elevated Levels of Phosphorylated Fibrinogen-α-Iso-forms and Differential Expression of Other Post-Translationally Modified Proteins in the Plasma of Ovarian Cancer PatientsJ. Proteome Res.2006,5, 3318−3325.
Journal of Proteome Research, 6(4), 1615–1615.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: January 18, 2021
2007 review
Epithelial ovarian cancer: Disease etiology, treatment, detection, and investigational gene, metabolite, and protein biomarkers
[Review of ]. JOURNAL OF PROTEOME RESEARCH, 6(8), 2936–2962.
Contributors: T. Williams n , K. Toups n, D. Saggese n, K. Kalli n, W. Cliby n & n
author keywords: biomarkers; epithelial ovarian cancer; proteomics; mass spectrometry; detection; treatments
MeSH headings : Biomarkers, Tumor / metabolism; Female; Humans; Neoplasms, Glandular and Epithelial / diagnosis; Neoplasms, Glandular and Epithelial / etiology; Neoplasms, Glandular and Epithelial / metabolism; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / etiology; Ovarian Neoplasms / metabolism
TL;DR:
An introduction to ovarian cancer is offered, with particular emphasis on human epithelial ovarian cancer, and a survey of promising new protein, gene, and metabolite biomarkers on the horizon is provided.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 article
New cavity rules
Yang, H. B., Hawkridge, A. M., Huang, S. P. D., Das, N., Bunge, S. D., Muddiman, D., & Stang, P. J. (2007, March). NATURE MATERIALS, Vol. 6, pp. 171–171.
MeSH headings : Alkynes / chemical synthesis; Alkynes / chemistry; Crystallography, X-Ray; Dendrimers / chemical synthesis; Dendrimers / chemistry; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Organometallic Compounds / chemical synthesis; Organometallic Compounds / chemistry; Organoplatinum Compounds / chemical synthesis; Organoplatinum Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
TL;DR:
Isotopically resolved mass spectrometry data support the existence of the metallodendrimers with rhomboidal and hexagonal cavities, and NMR data are consistent with the formation of all ensembles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Parts-per-billion mass measurement accuracy achieved through the combination of multiple linear regression and automatic gain control in a Fourier transform ion cyclotron resonance mass spectrometer
ANALYTICAL CHEMISTRY, 79(13), 5058–5063.
MeSH headings : Automation; Calibration; Cyclotrons / instrumentation; Ions; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Regression Analysis; Reproducibility of Results; Sensitivity and Specificity
TL;DR:
Calibrated multiple linear regression calibration is used to account for both differences in total ion population in the ICR cell as well as relative ion abundance of a given species, which affords mean MMA values at the parts-per-billion (ppb) level.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Probing the mechanisms of an air amplifier using a LTQ-FT-ICR-MS and fluorescence spectroscopy
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 18(11), 1909–1913.
MeSH headings : Air; Amplifiers, Electronic; Cyclotrons; Fluorescent Dyes / chemistry; Spectrometry, Fluorescence / instrumentation; Spectrometry, Fluorescence / methods; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods
TL;DR:
Experimental results provide direct evidence of aerodynamic focusing of electrosprayed droplets/ions enabled by an air amplifier, resulting in improved Electrospray droplet/ion capture efficiency and reduced ion injection time.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Quantitative comparison of a flared and a standard heated metal capillary inlet with a voltage-assisted air amplifier on an electrospray ionization linear ion trap mass spectrometer
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(19), 3207–3212.
MeSH headings : Air; Amplifiers, Electronic; Capillary Action; Electromagnetic Phenomena / instrumentation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis / instrumentation; Flow Injection Analysis / methods; Formates / analysis; Heating / instrumentation; Melitten / analysis; Microfluidics / instrumentation; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / instrumentation
TL;DR:
The performance characteristics of a flared and blunt-ended heated metal capillary were evaluated with a voltage-assisted air amplifier on a linear ion trap mass spectrometer and demonstrated that a standard capillary afforded higher ion abundance than a flared capillary.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Remote mass spectrometric sampling of electrospray- and desorption electrospra-generated ions using an air ejector
Journal of the American Society for Mass Spectrometry, 18(10), 1844–1847.
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2007 journal article
Remote mass spectrometric sampling of electrospray- and desorption electrospray-generated ions using an air ejector
Journal of the American Society for Mass Spectrometry, 18(10), 1844–1847.
MeSH headings : Air; Environmental Monitoring / instrumentation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis / instrumentation; Flow Injection Analysis / methods; Rheology / instrumentation; Rheology / methods; Robotics / instrumentation; Specimen Handling / instrumentation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
TL;DR:
This work demonstrates the remote analysis of a series of analyte ions that range from small molecules and polymers to polypeptides using the AE-LTQ interface and presents preliminary mass spectrometric data.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: February 24, 2020
2007 journal article
Sub parts-per-million mass measurement accuracy of intact proteins and product ions achieved using a dual electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometer
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 18(1), 1–7.
MeSH headings : Amino Acid Sequence; Calibration; Cyclotrons; Ions; Melitten / chemistry; Molecular Sequence Data; Nanotechnology; Polymers / chemistry; Propylene Glycols / chemistry; Proteomics; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Mass Spectrometry; Ubiquitin / chemistry
TL;DR:
While external calibration of protein fragments resulted in absolute MMA greater than 16 ppm, internal standardization significantly improved upon the MMA of both the intact proteins and their products ions which ranged from −2.0 ppm to 1.1 ppm.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2007 journal article
Understanding the influence of post-excite radius and axial confinement on quantitative proteomic measurements using Fourier transform ion cyclotron resonance mass spectrometry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(7), 1196–1204.
MeSH headings : Artifacts; Cyclotrons; Ions; Peptide Mapping / methods; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Sequence Analysis, Protein / methods; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods
TL;DR:
To adequately assess the multiple parameters in the large dataset, a multiplicative quality factor is developed that encompasses the total ion abundance, as well as the accuracy and the precision of abundance ratios, which allows facile determination of optimal instrument parameters for quantitative measurements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 article
"Lewis and Clark" proteomics
Muddiman, D. C. (2006, February). JOURNAL OF PROTEOME RESEARCH, Vol. 5, pp. 221–222.
MeSH headings : Animals; Biomarkers / chemistry; Blood Proteins / chemistry; Cells, Cultured; Disease Models, Animal; Famous Persons; History, 19th Century; Humans; Proteomics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 journal article
Accessible proteomics space and its implications for peak capacity for zero-, one- and two-dimensional separations coupled with FT-ICR and TOF mass spectrometry
JOURNAL OF MASS SPECTROMETRY, 41(3), 281–288.
MeSH headings : Biotechnology / instrumentation; Biotechnology / methods; Electrophoresis, Gel, Two-Dimensional; Fourier Analysis; Mass Spectrometry; Peptides / analysis; Proteomics / instrumentation; Proteomics / methods
TL;DR:
The results indicate that reverse-phase-nanoHPLC (RP-nHPLC) separation coupled with FT-ICR MS offers an order of magnitude improvement in peak capacity over RP- nHPLC separation coupling with TOF MS, and the addition of an orthogonal separation method, strong cation exchange (SCX), for 2D LC-MS demonstrates an additional 10-fold improvement inpeak capacity over 1D LC -MS methods.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 journal article
Chromatin Assembly Factor 1 Interacts with Histone H3 Methylated at Lysine 79 in the Processes of Epigenetic Silencing and DNA Repair†
Biochemistry, 45(9), 2852–2861.
MeSH headings : Amino Acid Sequence; Cell Extracts; Chromatin Assembly Factor-1; Chromosomal Proteins, Non-Histone / genetics; Chromosomal Proteins, Non-Histone / isolation & purification; Chromosomal Proteins, Non-Histone / metabolism; DNA Repair / physiology; DNA-Binding Proteins / genetics; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Epigenesis, Genetic / physiology; Gene Silencing; Histones / metabolism; Lysine / metabolism; Mass Spectrometry; Methylation; Molecular Sequence Data; Nucleosomes / metabolism; S Phase; Saccharomyces cerevisiae / metabolism; Silent Information Regulator Proteins, Saccharomyces cerevisiae / metabolism
TL;DR:
Together, these data indicate that CAF-1 interacts with H3 methylated at lysine 79 during the processes of epigenetic silencing and DNA repair.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2006 journal article
Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 20(22), 3409–3411.
MeSH headings : Bradykinin / analysis; Formates / analysis; Glucagon / analysis; Insulin / analysis; Melitten / analysis; Peptides / analysis; Proteins / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared; Vasodilator Agents / analysis
TL;DR:
The first coupling of a desorption electrospray ionization (DESI) ion source to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) for high-resolution protein analysis is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2006 journal article
Elevated levels of phosphorylated fibrinogen-alpha-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients
JOURNAL OF PROTEOME RESEARCH, 5(12), 3318–3325.
author keywords: ovarian cancer; biomarker; fibrinogen; two-dimensional gel electrophoresis; mass spectrometry; glycosylation; phosphorylation; protein depletion; Western blot
MeSH headings : Aged; Blood Proteins / analysis; Blotting, Western; Chromatography, High Pressure Liquid; Computational Biology; Electrophoresis, Gel, Two-Dimensional; Female; Fibrinopeptide A / analysis; Humans; Mass Spectrometry; Middle Aged; Ovarian Neoplasms / blood
TL;DR:
This work evaluated the differentially expressed proteins in the plasma of ovarian cancer patients using 2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (P...
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 journal article
Focus in Honor of James A. McCloskey, Recipient of the 2005 ASMS award for a distinguished contribution in mass spectrometry
Journal of the American Society for Mass Spectrometry, 17(10), I1–I2.
MeSH headings : Awards and Prizes; Biochemistry; Chemistry, Analytic; History, 20th Century; History, 21st Century; Mass Spectrometry; Nucleic Acids / analysis; Texas; Utah
Sources: Crossref, ORCID
Added: February 24, 2020
2006 journal article
Generation and detection of multiply-charged peptides and proteins by matrix-assisted laser desorption electrospray ionization (MALDESI) Fourier transform ion cyclotron resonance mass spectrometry
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 17(12), 1712–1716.
MeSH headings : Algorithms; Cyclotrons; Peptide Mapping / methods; Peptides / analysis; Proteins / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Static Electricity
TL;DR:
Analysis of peptides and proteins resulted in high resolving power single-acquisition FT-ICR mass spectra with average charge-states highly correlated to those obtained by nanoESI, thus, providing strong evidence that the ESI process dictates the observed charge-state distribution.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 journal article
Identification of Subunit−Subunit Interactions in Bacteriophage P22 Procapsids by Chemical Cross-linking and Mass Spectrometry
Journal of Proteome Research, 5(2), 370–377.
author keywords: bacteriophage P22; capsid interactions; chemical cross-linking; mass spectrometry
MeSH headings : Amino Acid Substitution / genetics; Bacteriophage P22 / metabolism; Capsid / metabolism; Capsid Proteins / genetics; Capsid Proteins / metabolism; Cross-Linking Reagents; Cysteine / chemistry; Cysteine / genetics; Mass Spectrometry; Models, Molecular; Protein Binding; Protein Subunits / metabolism; Threonine / chemistry; Threonine / genetics; Virus Assembly
TL;DR:
To build a detailed structure model, the secondary structure elements of the P22 coat protein are predicted, and the prediction onto identified helical elements of cryoEM 3D reconstruction is attempted.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2006 journal article
Incorporation of 2,6-di(4,4 '-dipyridyl)-9-thiabicyclo[3.3.1] nonane into discrete 2D supramolecules via coordination-driven self-assembly
JOURNAL OF ORGANIC CHEMISTRY, 71(17), 6644–6647.
MeSH headings : Bridged Bicyclo Compounds / chemistry; Molecular Structure; Platinum / chemistry; Pyridines / chemistry; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared
TL;DR:
The synthesis and characterization of three new supramolecular complexes 6-8 (a rhomboid and two hexagons) via coordination-driven self-assembly are reported in excellent yields (>90%) and the X-ray structure of the 120 degrees tecton 2 is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 journal article
Molecular Architecture via Coordination: Self-Assembly of Nanoscale Hexagonal Metallodendrimers with Designed Building Blocks
Journal of the American Chemical Society, 128(31), 10014–10015.
MeSH headings : Dendrimers / chemistry; Metals / chemistry; Models, Molecular; Nanotechnology
TL;DR:
The first self-assembly of nanoscale metallodendrimers that have a hexagonal cavity as a core via the directional-bonding approach is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
11. Sustainable Cities and Communities
(OpenAlex)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018
2006 journal article
Molecular Architecture via Coordination: Self-Assembly of Nanoscale Hexagonal Metallodendrimers with Designed Building Blocks
Journal of American Chemical Society, 128(31), 10014–10015.
UN Sustainable Development Goal Categories
11. Sustainable Cities and Communities
(OpenAlex)
Sources: NC State University Libraries, ORCID
Added: March 8, 2021
2005 journal article
Accurate mass precursor ion data and tandem mass spectrometry identify a class I human leukocyte antigen A*0201-presented peptide originating from vaccinia virus
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 16(11), 1812–1817.
MeSH headings : Amino Acid Sequence; HLA Antigens / chemistry; HLA Antigens / immunology; HeLa Cells; Humans; Immunoassay / methods; Microchemistry / methods; Molecular Sequence Data; Nucleoside-Phosphate Kinase / chemistry; Nucleoside-Phosphate Kinase / immunology; Peptides / analysis; Peptides / chemistry; Protein Precursors / analysis; Protein Precursors / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Vaccinia virus / chemistry; Vaccinia virus / immunology; Viral Proteins / analysis; Viral Proteins / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2005 journal article
Coordination-Driven Self-Assemblies with a Carborane Backbone
Journal of the American Chemical Society, 127(34), 12131–12139.
MeSH headings : Boranes / chemistry; Crystallography, X-Ray; Isotope Labeling; Magnetic Resonance Spectroscopy; Molecular Structure; Pyridines / chemistry; Spectrometry, Mass, Electrospray Ionization
TL;DR:
Isotopically resolved mass spectrometry data confirm the existence of the rectangle, triangle, and hexagon, and NMR data are consistent with the formation of all five assemblies.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Derivatives of pentamidine designed to target the Leishmania lipophosphoglycan
Tetrahedron Letters, 46(4), 695–698.
author keywords: boronate drug targeting; pentamidine; Leishmania
TL;DR:
Pentamidine has been modified with boronic acids so that it might bind more selectively to the phosphodisaccharide repeating unit of the LPG, which could serve to target the drug to the protozoan surface and increase its efficacy in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Déjà Vu All Over Again: Skin Cap Still Contains a High-Potency Glucocorticosteroid
Archives of Dermatology, 141(6).
MeSH headings : Administration, Topical; Adrenal Cortex Hormones / adverse effects; Adrenal Cortex Hormones / chemistry; Adrenal Cortex Hormones / therapeutic use; Chemistry, Pharmaceutical; Dermatologic Agents / adverse effects; Dermatologic Agents / pharmacology; Dermatologic Agents / therapeutic use; Female; Humans; Male; Materials Testing; Psoriasis / diagnosis; Psoriasis / drug therapy; Risk Assessment; Sensitivity and Specificity
Sources: Crossref, ORCID
Added: August 28, 2020
2005 journal article
Effect of post-excitation radius on ion abundance, mass measurement accuracy, and isotopic distributions in Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 19(7), 915–918.
MeSH headings : Bradykinin / analysis; Ions; Isotopes; Reproducibility of Results; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tritium
TL;DR:
The findings presented herein underscore the importance of determining the optimal post-excitation radius in FT-ICR-MS to achieve high ion abundance (low limits of detection), high MMA, and valid isotopic distributions.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Evaluation of Protein Depletion Methods for the Analysis of Total-, Phospho- and Glycoproteins in Lumbar Cerebrospinal Fluid
Journal of Proteome Research, 4(3), 837–845.
author keywords: cerebrospinal fluid; human; two-dimensional gel electrophoresis; mass spectrometry; glycoprotein; phosphoprotein; protein depletion; low-abundance protein
MeSH headings : Adult; Aged; Cerebrospinal Fluid Proteins / analysis; Cerebrospinal Fluid Proteins / classification; Cerebrospinal Fluid Proteins / isolation & purification; Chromatography, Affinity; Electrophoresis, Gel, Two-Dimensional; Female; Fluorescent Dyes; Glycoproteins / cerebrospinal fluid; Glycoproteins / isolation & purification; Humans; Immunoglobulins; Male; Mass Spectrometry; Methods; Microspheres; Middle Aged; Phosphoproteins / cerebrospinal fluid; Phosphoproteins / isolation & purification
TL;DR:
Phospho- and glycoprotein specific fluorescent stains and mass spectrometry were used to map proteins from CSF on two-dimensional gel electropherograms after immunoaffinity based protein removal, and fifty proteins were identified from 66 spots, and 12 proteins have not been annotated in previously published 2D gels.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Gentle Protein Ionization Assisted by High-Velocity Gas Flow
Analytical Chemistry, 77(19), 6174–6183.
MeSH headings : Cytochromes c / chemistry; Hydrogen-Ion Concentration; Ions / chemistry; Myoglobin / chemistry; Proteins / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Ubiquitin / chemistry
TL;DR:
Evidence is provided for the importance of the conditions used in the desolvation process for the preservation of the protein conformation and suggest that the conditions achieved when using high-velocity gas flows to assist droplet evaporation and ion Desolvation are much gentler than those in conventional ESI experiments.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Identification of class IIHLA-DBR1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry
JOURNAL OF PROTEOME RESEARCH, 4(6), 2243–2249.
author keywords: measles; HLA class II peptides; 2D-LC; tandem mass spectrometry; MS/MS
MeSH headings : Amino Acid Sequence; Cancer Vaccines / chemistry; Cations; Chromatography; Chromatography, Ion Exchange; Chromatography, Liquid / methods; Databases, Factual; Databases, Protein; HLA Antigens / chemistry; HLA-DR Antigens / genetics; HLA-DRB1 Chains; Hemagglutinins / chemistry; Humans; Leukocytes, Mononuclear / metabolism; Mass Spectrometry / methods; Measles Vaccine / chemistry; Measles virus / genetics; Molecular Sequence Data; Nucleocapsid Proteins / chemistry; Peptides / chemistry; Peptides / genetics; Phosphoproteins / chemistry; Proteomics / methods; Sequence Homology, Amino Acid; Time Factors
TL;DR:
Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2005 journal article
Leveling response factors in the electrospray ionization process using a heated capillary interface
Journal of the American Society for Mass Spectrometry, 16(5), 772–778.
MeSH headings : Capillary Action; DNA / analysis; DNA / chemistry; Equipment Design; Equipment Failure Analysis; Hot Temperature; Humans; Specimen Handling / instrumentation; Specimen Handling / methods; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The implementation of a heated electrospray source was used to denature amplicons containing 7-deaza purines, which can be used to facilitate sequencing by mass spectrometry.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Mapping Sites of Protein Phosphorylation by Mass Spectrometry Utilizing a Chemical-Enzymatic Approach: Characterization of Products from α-S1Casein Phosphopeptides
Journal of Proteome Research, 4(2), 424–434.
author keywords: chemical-enzymatic; phosphorylation; 2-aminoethanethiol; isotope dilution mass spectrometry; phosphoproteins; phosphopeptides; quantification
MeSH headings : Amino Acid Sequence; Animals; Caseins / metabolism; Cattle; Molecular Sequence Data; Peptide Fragments / metabolism; Phosphopeptides / metabolism; Phosphorylation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
ESI-TOF mass spectrometry was used to characterize products from the chemical-enzymatic cleavage of specific phosphorylation sites in bovine alpha-S1 casein and synthetic phosphopeptides containing substitutions at a single phosphorylated site.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2005 journal article
Normalization of relative peptide ratios derived from in-gel digests: applications to protein variant analysis at the peptide level
Rapid Communications in Mass Spectrometry, 19(19), 2871–2877.
MeSH headings : Algorithms; Chromatography, High Pressure Liquid / methods; Gas Chromatography-Mass Spectrometry / methods; Gas Chromatography-Mass Spectrometry / standards; Humans; Osmotic Fragility; Peptide Mapping / methods; Peptide Mapping / standards; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Spectrin / analysis; Spectrin / chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A strategy was developed to normalize for sample variability due to ionization efficiency, solvent effects, digestion and extraction efficiency by utilizing multiple isotopically labeled internal standards for multiple peptides derived from the same protein.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Nucleic Acid Analysis by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at the Beginning of the Twenty-First Century
Current Pharmaceutical Design, 11(20), 2593–2613.
author keywords: FT-ICR; Fourier transform ion cyclotron resonance; mass spectrometry; DNA; RNA; PNA
MeSH headings : Nucleic Acids / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Electrospray Ionization / trends; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / trends; Spectroscopy, Fourier Transform Infrared / methods; Spectroscopy, Fourier Transform Infrared / trends; Spectrum Analysis / methods; Spectrum Analysis / trends; Technology, Pharmaceutical / instrumentation; Technology, Pharmaceutical / methods; Technology, Pharmaceutical / trends
TL;DR:
A diverse range of studies including fundamental studies of nucleic acid properties, improvements in sample clean up and preparation, better methods to obtain higher mass measurement accuracy, analysis of noncovalent complexes, tandem mass spectrometry, and characterization of peptide nucleic acids are presented.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2005 journal article
Primary Trabecular Meshwork Cells Incubated in Human Aqueous Humor Differ from Cells Incubated in Serum Supplements
Investigative Opthalmology & Visual Science, 46(8), 2848.
MeSH headings : Adult; Aged; Aged, 80 and over; Aqueous Humor / physiology; Ascorbic Acid / metabolism; Ascorbic Acid / pharmacology; Blotting, Western; Cell Culture Techniques; Cell Proliferation; Culture Media; Cytoskeletal Proteins / metabolism; Dexamethasone / pharmacology; Electrophoresis, Gel, Two-Dimensional; Eye Proteins / metabolism; Female; Glycoproteins / metabolism; Humans; Infant; Male; Middle Aged; Serum; Tissue Inhibitor of Metalloproteinase-1 / metabolism; Trabecular Meshwork / cytology; Trabecular Meshwork / drug effects; Trabecular Meshwork / metabolism
TL;DR:
Addition of human aqueous humor rather than FBS to trabecular monolayer cell cultures triggers significant changes in cellular and molecular characteristics.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2005 journal article
Protein expression profiling of CLL B cells using replicate off-line strong cation exchange chromatography and LC–MS/MS
Journal of Chromatography B, 819(1), 33–39.
author keywords: CLL; 2D-LC-MS/MS; B cell; expression profiling; off-line SCX; reproducibility
MeSH headings : Antigens, CD / isolation & purification; B-Lymphocytes / metabolism; Blood Proteins / isolation & purification; Cell Fractionation; Cell Membrane / chemistry; Chromatography, Ion Exchange / methods; Chromatography, Liquid / methods; Gene Expression Profiling / methods; Humans; Leukemia, Lymphocytic, Chronic, B-Cell / metabolism; Mass Spectrometry / methods; Neoplasm Proteins / blood
TL;DR:
Examination of crude membranes from B cells derived from a patient with chronic lymphocytic leukemia to examine the protein expression profile of CLL B cells shows an overlap of 64% between proteins identified in replicate runs was achieved for this system.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2005 journal article
Quantitative Protein Expression Analysis Of CLL B Cells from Mutated and Unmutated IgVHSubgroups Using Acid-Cleavable Isotope-Coded Affinity Tag Reagents
Journal of Proteome Research, 4(4), 1310–1317.
author keywords: CLL; B cell; quantitative comparison; clCAT; UM-CLL; M-CLL; linear ion trap mass spectrometer
MeSH headings : B-Lymphocytes / chemistry; B-Lymphocytes / immunology; Cell Fractionation; Genes, Immunoglobulin; Humans; Immunoglobulin Heavy Chains / genetics; Immunoglobulin Heavy Chains / immunology; Immunoglobulin Variable Region / genetics; Immunoglobulin Variable Region / immunology; Isotope Labeling / methods; Leukemia, Lymphocytic, Chronic, B-Cell / immunology; Mass Spectrometry / methods; Mutation; Proteome / analysis
TL;DR:
Stable isotope labeling and mass spectrometry can complement 2D gel electrophoresis and gene microarray technologies for identifying putative and perhaps unique prognostic markers in CLL.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 18, 2021
2005 journal article
Quantitative mass spectral evidence for the absence of circulating brain natriuretic peptide (BNP-32) in severe human heart failure
Proceedings of the National Academy of Sciences, 102(48), 17442–17447.
author keywords: biomarkers; electrospray ionization; Fourier transform-ion cyclotron resonance MS; immunoaffinity purification; targeted proteomics
MeSH headings : Chromatography, Liquid; Female; Heart Failure / metabolism; Humans; Isotope Labeling; Male; Mass Spectrometry; Natriuretic Peptide, Brain / blood; Natriuretic Peptide, Brain / isolation & purification
TL;DR:
An immunoaffinity purification assay is developed and used to isolate endogenous BNP-32 from New York Heart Association class IV patient plasma for subsequent analysis by nano-liquid chromatography (LC) electrospray ionization Fourier transform ion cyclotron resonance (FT-ICR) MS.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 24, 2020
2005 journal article
Reproducibility of Retention Time using a Splitless nanoLC Coupled to an ESI-FTICR Mass Spectrometer
Journal of Biomolecular Techniques, 16, 4, 414–422.
Source: NC State University Libraries
Added: March 17, 2021
2005 journal article
Statistical Evaluation of Internal and External Mass Calibration Laws Utilized in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Chemistry, 77(8), 2406–2414.
MeSH headings : Calibration; Cyclotrons; Data Interpretation, Statistical; Fourier Analysis; Polymers / analysis; Polymers / chemistry; Propylene Glycols / analysis; Propylene Glycols / chemistry; Quaternary Ammonium Compounds / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The statistical evaluation of two common and three new calibration laws utilized in Fourier transform ion cyclotron resonance mass spectrometry are presented and the number of calibrant ions as well as their required m/z range is investigated in order to successfully achieve high MMA.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2004 journal article
A method for calculating 16o/18o peptide ion ratios for the relative quantification of proteomes
Journal of the American Society for Mass Spectrometry, 15(4), 437–445.
MeSH headings : Amino Acid Sequence; Animals; Apoproteins / analysis; Apoproteins / chemistry; Cattle; Mass Spectrometry; Molecular Sequence Data; Oxygen Isotopes; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Serum Albumin / analysis; Serum Albumin / chemistry; Spectroscopy, Fourier Transform Infrared; Transferrin / analysis; Transferrin / chemistry
TL;DR:
The data demonstrate that for biomarker discovery, it is advantageous to label the proteome representing the disease state with 18O; and the method is not sensitive to variations in 18O1/18O2 ratio.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 24, 2020
2004 journal article
Absolute Quantification of the Model Biomarker Prostate-Specific Antigen in Serum by LC−MS/MS Using Protein Cleavage and Isotope Dilution Mass Spectrometry
Journal of Proteome Research, 3(3), 644–652.
author keywords: LC-MS/MS; absolute quantification; serum; biomarker; prostate-specific antigen; protein
MeSH headings : Humans; Isotope Labeling; Male; Mass Spectrometry; Peptide Fragments / chemistry; Prostate-Specific Antigen / chemistry; Protein Processing, Post-Translational; Serum / chemistry; Trypsin / chemistry; Urea / chemistry
TL;DR:
absolute quantification can be performed on the model biomarker PSA introduced into denatured serum when analyzed by LC-MS/MS, and concerns still exist regarding sensitivity compared to existing immunoassays as well as the reproducibility of PC-IDMS performed in different matrixes.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2004 conference paper
Accurate and Expedited Diagnosis of Amyloidotic Transthyretin Neuropathy: Proteomic and Genomic Approach
Annals of Neurology, 56(S8), 29.
Event: American Neurological Association, 129th Annual Meeting at Toronto, Ontario, Canada on October 3-6, 2004
Source: NC State University Libraries
Added: March 17, 2021
2004 journal article
Analysis of the Low Molecular Weight Fraction of Serum by LC-Dual ESI-FT-ICR Mass Spectrometry: Precision of Retention Time, Mass, and Ion Abundance
Analytical Chemistry, 76(17), 5097–5103.
MeSH headings : Algorithms; Biomarkers, Tumor / blood; Chromatography, Liquid / standards; Female; Humans; Ions / blood; Molecular Weight; Ovarian Neoplasms / blood; Reproducibility of Results; Serum / chemistry; Software; Spectrometry, Mass, Electrospray Ionization / standards
TL;DR:
These measures of analytical reproducibility establish a statistical rationale for differentiating healthy and disease patient populations for the elucidation of biomarkers in the low molecular fraction of serum.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2004 journal article
Analytical Performance of a Venturi Device Integrated into an Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Analysis of Nucleic Acids
Analytical Chemistry, 76(14), 4118–4122.
MeSH headings : Cyclotrons; Fourier Analysis; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Oligonucleotides / analysis; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
A voltage-assisted venturi device modeled after an industrial air amplifier was used to improve the ion transmission efficiency of a 16.2 kDa oligonucleotide and a 53-mer PCR product in the high-pressure region between an electrospray ionization emitter and the sampling orifices of two Fourier transform ion cyclotron resonance mass spectrometers (FT-ICR-MS).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, ORCID
Added: January 18, 2021
2004 journal article
Androgens Negatively Regulate Forkhead Transcription Factor FKHR (FOXO1) through a Proteolytic Mechanism in Prostate Cancer Cells
Journal of Biological Chemistry, 279(14), 13866–13877.
MeSH headings : Amino Acid Sequence; Androgens / pharmacology; Apoptosis / drug effects; Apoptosis / physiology; Cell Line, Tumor / cytology; Cell Line, Tumor / drug effects; Cell Line, Tumor / physiology; Cell Nucleus / metabolism; Cysteine Endopeptidases / metabolism; Forkhead Box Protein O1; Forkhead Transcription Factors; Gene Expression Regulation, Neoplastic / drug effects; Humans; Male; Metribolone / pharmacology; Molecular Sequence Data; Mutagenesis; Prostatic Neoplasms; Protease Inhibitors / pharmacology; RNA, Messenger / analysis; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation / drug effects; Transfection
TL;DR:
This work demonstrates that forkhead transcription factor FKHR (FOXO1)-induced death of LNCaP cells was blocked by a synthetic androgen R1881, and suggests that androgens induce increased activity of an acidic cysteine protease, which in turn cleaves FK HR.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 24, 2020
2004 journal article
Detection of Genetic Variants of Transthyretin by Liquid Chromatography–Dual Electrospray Ionization Fourier-Transform Ion-Cyclotron-Resonance Mass Spectrometry
Clinical Chemistry, 50(9), 1535–1543.
MeSH headings : Amyloidosis, Familial / genetics; Gas Chromatography-Mass Spectrometry / instrumentation; Gas Chromatography-Mass Spectrometry / methods; Humans; Point Mutation; Prealbumin / chemistry; Prealbumin / genetics; Sequence Analysis, DNA; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
FT-ICR mass spectrometry has sufficient mass accuracy to identify genetic variants of immunoaffinity-purified TTR and discovered and characterized a previously unreported cis double mutation with a mass only 2 Da different from wild-type TTR.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2004 journal article
Determination of the relative energies of activation for the dissociation of aromatic versus aliphatic phosphopeptides by ESI-FTICR-MS and IRMPD
Journal of the American Society for Mass Spectrometry, 15(1), 121–127.
MeSH headings : Peptide Mapping / methods; Phosphopeptides / analysis; Phosphopeptides / chemistry; Protein Structure, Secondary; Serine / analysis; Serine / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Thermodynamics; Tyrosine / analysis; Tyrosine / chemistry
TL;DR:
The data presented in this manuscript support the theory that for phosphopeptides, the phosphate moiety’s P-O stretch is in direct resonance with the infrared laser, thus increasing the relative absorptivity of the modified species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, ORCID
Added: February 24, 2020
2004 journal article
Discovery of Ovarian Cancer Biomarkers in Serum Using NanoLC Electrospray Ionization TOF and FT-ICR Mass Spectrometry
Disease Markers, 19(4-5), 239–249.
MeSH headings : Amino Acid Sequence; Biomarkers, Tumor / blood; Chromatography; Female; Humans; Middle Aged; Molecular Sequence Data; Nanotechnology; Neoplasm Staging; Ovarian Neoplasms / blood; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization
TL;DR:
A set of protocols for the isolation and identification of serum biomarkers for ovarian cancer that exist in the low molecular weight serum fraction is delineated and one of these contains the sequence of fibrinopeptide-A known to be elevated in many types of cancer including ovarian cancer.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2004 journal article
Evaluation of a Cleavable Stable Isotope Labeled Synthetic Peptide for Absolute Protein Quantification Using LC−MS/MS
Journal of Proteome Research, 3(3), 658–661.
author keywords: LC-MS/MS; absolute quantification; biomarker; cleavable internal standard
MeSH headings : Chromatography, Liquid; Isotope Labeling; Mass Spectrometry; Peptides / chemistry
TL;DR:
The work presented here evaluates the use of tryptic cleavage sites incorporated into the internal standard synthetic peptide in an attempt to create an internal standard that has cleavage characteristics more similar to the protein being quantified.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 28, 2020
2004 journal article
Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides
Journal of Virology, 78(1), 42–51.
MeSH headings : Alleles; Cytokines / biosynthesis; Cytokines / genetics; HLA-DR Antigens / chemistry; HLA-DR Antigens / metabolism; HLA-DRB1 Chains; Humans; Interferon-gamma / biosynthesis; Interferon-gamma / genetics; Interleukin-4 / biosynthesis; Interleukin-4 / genetics; Measles / immunology; Measles / prevention & control; Measles virus / immunology; Measles virus / metabolism; Measles-Mumps-Rubella Vaccine / administration & dosage; Nucleocapsid Proteins; Nucleoproteins / chemistry; Nucleoproteins / immunology; Nucleoproteins / metabolism; Peptides / chemical synthesis; Peptides / chemistry; Peptides / immunology; Peptides / metabolism; T-Lymphocytes / immunology; Viral Proteins / chemistry; Viral Proteins / immunology; Viral Proteins / metabolism
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
2004 journal article
Identification of Transthyretin Variants by Sequential Proteomic and Genomic Analysis
Clinical Chemistry, 50(9), 1544–1552.
MeSH headings : Amyloidosis, Familial / diagnosis; Amyloidosis, Familial / genetics; DNA / chemistry; DNA / genetics; Gas Chromatography-Mass Spectrometry / instrumentation; Gas Chromatography-Mass Spectrometry / methods; Genomics / methods; Genotype; Humans; Polymerase Chain Reaction; Polymorphism, Genetic; Prealbumin / chemistry; Prealbumin / genetics; Proteomics / methods; Sequence Analysis, DNA; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
This method correctly identified all previously known variants and also identified a novel variant, Val94Ala, which allows screening for the familial forms of amyloidosis in a broad patient population in a timely fashion.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2004 journal article
Informed use of proteolytic inhibitors in biomarker discovery
Rapid Communications in Mass Spectrometry, 18(9), 1001–1002.
MeSH headings : Biomarkers / analysis; Combinatorial Chemistry Techniques; Drug Design; Prealbumin / analysis; Protease Inhibitors / chemistry; Spectrometry, Mass, Electrospray Ionization / methods
Sources: Crossref, ORCID
Added: February 24, 2020
2004 journal article
Utility of accurate monoisotopic mass measurements to confidently identify lambda exonuclease generated single-stranded amplicons containing 7-deaza analogs by electrospray ionization FT-ICR mass spectrometry
International Journal of Mass Spectrometry, 234(1-3), 79–87.
Sources: Crossref, ORCID
Added: January 18, 2021
2003 journal article
1H NMR and electrospray mass spectrometry of the mono-ionized bis(2,2′-bis(4,5-dimethylimidazole)chloronitrosylruthenium(II) complex ([Ru(NO)Cl(LH2)2]+, LH2=2,2′-bis(4,5-dimethylimidazole)
Inorganica Chimica Acta, 343, 317–328.
author keywords: ruthenium complexes; nitrosyl complexes; imidazole complexes; electrospray mass spectrometry
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: February 27, 2021
2003 journal article
Advantages of Thermococcus kodakaraenis (KOD) DNA Polymerase for PCR-mass spectrometry based analyses
Journal of the American Society for Mass Spectrometry, 14(6), 601–604.
MeSH headings : DNA-Directed DNA Polymerase / chemistry; Fourier Analysis; Humans; Polymerase Chain Reaction / methods; Spectrometry, Mass, Electrospray Ionization; Taq Polymerase / chemistry; Thermococcus / enzymology; von Willebrand Factor / chemistry
TL;DR:
KOD DNA polymerase demonstrated a 2- to 3-fold increase in PCR product formation compared to Pfu or Taq, respectively, and generated blunt-ended PCR product which allows facile interpretation of the mass spectrum.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Cancer Biomarkers: How Proteomics is Leading to the Discovery of New Markers
Clinical Laboratory News, 29, 12–16.
Source: NC State University Libraries
Added: March 17, 2021
2003 journal article
Coordination-Driven Self-Assembly of Supramolecular Cages: Heteroatom-Containing and Complementary Trigonal Prisms
Journal of the American Chemical Society, 125(32), 9647–9652.
MeSH headings : Anthracenes / chemical synthesis; Anthracenes / chemistry; Benzene Derivatives / chemical synthesis; Benzene Derivatives / chemistry; Cross-Linking Reagents / chemical synthesis; Cross-Linking Reagents / chemistry; Crystallography, X-Ray; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; Organosilicon Compounds / chemical synthesis; Organosilicon Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
TL;DR:
The self-assembly of three nanoscopic prisms of approximate size 1 x 4 nm is reported, which completes a pair of nanoscopic complementary 3-D structures.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 17(15), 1714–1722.
MeSH headings : Humans; Molecular Weight; Nucleic Acids / analysis; Polymerase Chain Reaction; Proteins / analysis; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Repeat Sequences / genetics
TL;DR:
A practical correction factor is developed that is applied to the ToC measurement to largely remove the systematic bias introduced by nature and accurately predicts resolving power achievable for Fourier transform ion cyclotron resonance (FT-ICR) mass analysis of large molecules.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Dual Electrospray Ionization Source for Confident Generation of Accurate Mass Tags Using Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Chemistry, 75(14), 3411–3418.
MeSH headings : Amino Acid Sequence; Apoproteins / chemistry; Chromatography, Liquid; Cyclotrons; Mass Spectrometry; Molecular Sequence Data; Myoglobin / chemistry; Proteins / chemistry; Proteomics / instrumentation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectroscopy, Fourier Transform Infrared / instrumentation
TL;DR:
It is argued that the most effective way to account for space-charge effects is to introduce an internal mass calibrant (IMC) using a dual electrospray ionization (ESI) source where the IMC is added from a separate ESI emitter.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Enzymatic strategies for the characterization of nucleic acids by electrospray ionization mass spectrometry
Rapid Communications in Mass Spectrometry, 17(24), 2699–2706.
MeSH headings : Exodeoxyribonucleases / chemistry; Isotope Labeling / methods; Nucleic Acids / analysis; Nucleic Acids / chemistry; Oligonucleotides / analysis; Oligonucleotides / chemistry; Polymerase Chain Reaction / methods; Polymorphism, Single Nucleotide; Reproducibility of Results; Restriction Mapping / methods; Sensitivity and Specificity; Sequence Alignment / methods; Sequence Analysis, DNA / methods; Spectrometry, Mass, Electrospray Ionization / methods; Viral Proteins
TL;DR:
This work describes an eclectic array of enzymatic strategies invoked in order to detect single-nucleotide polymorphisms by ESI-MS, although other applications may be envisioned.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2003 journal article
Implications of Hydrophobicity and Free Energy of Solvation for Characterization of Nucleic Acids by Electrospray Ionization Mass Spectrometry
Analytical Chemistry, 75(6), 1331–1339.
MeSH headings : Deoxyribonucleotides / analysis; Gene Frequency; Hydrophobic and Hydrophilic Interactions; Nucleic Acids / chemistry; Oligonucleotides / analysis; Polymorphism, Single Nucleotide; Solvents; Spectrometry, Mass, Electrospray Ionization / methods; Thermodynamics
TL;DR:
Investigation of the contributions of hydrophobicity and free energy of solvation to relative signal intensities in ESI-MS spectra of nucleic acids found a trend in signal intensity for deoxyribonucleotide triphosphates, oligonucleotides, and PCR amplicons was observed that was consistent with the model.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Mass spectral determination of skeletal/cardiac actin isoform ratios in cardiac muscle
Rapid Communications in Mass Spectrometry, 17(13), 1467–1471.
MeSH headings : Actins / analysis; Animals; Cattle; Mass Spectrometry; Muscle, Skeletal / chemistry; Myocardium / chemistry; Protein Isoforms / analysis; Rabbits
TL;DR:
A mass spectrometric technique to measure the ratio of skeletal to cardiac actin and a new dual electrospray ionization source was employed to determine accurate masses of the alpha-skeletal and alpha-cardiac actins.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood
Virology, 312(2), 495–506.
author keywords: measles-derived peptide; HLA-DRB1*03; measles virus; mass spectrometry; lymphocyte proliferation
MeSH headings : Adolescent; Amino Acid Sequence; Antigen Presentation; Antigens, Viral / analysis; Antigens, Viral / chemistry; Antigens, Viral / immunology; Antigens, Viral / metabolism; Child; Female; HLA-DR Antigens / immunology; HLA-DR Antigens / metabolism; HLA-DRB1 Chains; Humans; Male; Mass Spectrometry; Measles Vaccine / immunology; Measles virus / chemistry; Measles virus / metabolism; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / immunology; Peptides / metabolism; T-Lymphocytes / immunology
TL;DR:
This data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
CEPH family 1362 STR database: An online resource for characterization of PCR products using electrospray ionization mass spectrometry
Journal of the American Society for Mass Spectrometry, 13(1), 89–90.
MeSH headings : DNA, Complementary / chemistry; Databases, Genetic; Genetic Markers; Humans; Internet; Polymerase Chain Reaction / methods; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Repeat Sequences / genetics
TL;DR:
An online database has been established in order to validate electrospray ionization mass spectrometry (ESI-MS) for genotyping and to publicize the procedures developed in the laboratory for the characterization of PCR products by ESI-MS.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
Dual-micro-ESI source for precise mass determination on a quadrupole time-of-flight mass spectrometer for genomic and proteomic applications
Analytical and Bioanalytical Chemistry, 373(7), 538–546.
author keywords: electrospray; time-of-flight; mass spectrometry; internal calibration; accurate mass
MeSH headings : Amino Acid Sequence; Calibration; Caseins / chemistry; Caseins / metabolism; Genomics / methods; Ions; Molecular Sequence Data; Phosphoproteins / chemistry; Phosphoproteins / metabolism; Polymerase Chain Reaction; Proteome; Sensitivity and Specificity; Sequence Analysis, Protein; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Trypsin / metabolism
TL;DR:
In this report, the dual-sprayer is demonstrated in the negative-ion mode for internal calibration of polymerase chain reaction (PCR) amplicons generated from synthetic and genomic templates as well as a proteolytic digest of a naturally phosphorylated protein.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
Evaluation of sample preparation techniques for mass measurements of PCR products using ESI-FT-ICR mass spectrometry
Journal of the American Society for Mass Spectrometry, 13(4), 338–344.
MeSH headings : 2-Propanol / chemistry; 2-Propanol / isolation & purification; DNA / chemistry; Ethanol / chemistry; Ethanol / isolation & purification; Indicators and Reagents; Microdialysis; Polymerase Chain Reaction / methods; Solvents; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
Alternative cleanup techniques that are compatible with automation are explored here with the intent of expanding the bottleneck that exists between the production of PCR products and analysis by electrospray ionization mass spectrometry (ESI-MS).
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2002 journal article
Gas-Phase Ion Unimolecular Dissociation for Rapid Phosphopeptide Mapping by IRMPD in a Penning Ion Trap: An Energetically Favored Process
Journal of the American Chemical Society, 124(23), 6546–6547.
MeSH headings : Amino Acid Sequence; Lasers; Peptide Mapping / methods; Phosphopeptides / chemistry; Receptor, Insulin / chemistry; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / methods; Thermodynamics
TL;DR:
This research demonstrates that the energy of activation for dissociation of the phosphopeptides is lower than that of the unmodified analogues providing the basis for the success of this technique, and IRMPD of ions in a Penning ion trap is an ideal platform for rapid phosphopeptic mapping.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
In celebration of the 70th birthday of Dr David M. Hercules
Analytical and Bioanalytical Chemistry, 373(7), 517–518.
MeSH headings : Chemistry, Analytic / history; History, 20th Century; History, 21st Century; Spectrum Analysis; United States
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
Memoirs, Hercules lineage, students, visiting scientists and staff
Analytical and Bioanalytical Chemistry, 373(7), 669–675.
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
Solution composition and thermal denaturation for the production of single-stranded PCR amplicons: Piperidine-induced destabilization of the DNA duplex?
Journal of the American Society for Mass Spectrometry, 13(3), 232–240.
MeSH headings : Cyclotrons; DNA / chemistry; DNA / drug effects; DNA, Single-Stranded / chemistry; Fourier Analysis; Hot Temperature; Humans; Nucleic Acid Denaturation / drug effects; Oligonucleotides / chemistry; Piperidines / pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization
TL;DR:
The volatile base piperidine, present at 20 mM concentrations in the electrospray solution, was found to play a critical role in the formation of single-stranded species at the higher aqueous percentages and a duplex destabilization mechanism has been proposed.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2002 journal article
Tailoring the gas-phase dissociation and determining the relative energy of activation for dissociation of 7-deaza purine modified oligonucleotides containing a repeating motif
International Journal of Mass Spectrometry, 219(1), 139–150.
TL;DR:
7-Deaza purine modified oligonucleotides with a repeating motif have been sequenced by slow heating methods illustrating the ability to alter the preferred unimolecular decomposition pathway and limited the number of product ions per repeat unit resulting in an increased signal-to-noise ratio for the tandem mass spectrometry data.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, ORCID
Added: February 27, 2021
2001 journal article
Complete sequencing of mono—deprotonated peptide nucleic acids by sustained off-resonance irradiation collision—induced dissociation
Journal of the American Society for Mass Spectrometry, 12(7), 805–809.
MeSH headings : Calibration; Chemical Phenomena; Chemistry, Physical; Mass Spectrometry; Peptide Nucleic Acids / chemistry; Sequence Analysis, DNA
TL;DR:
Complete peptide nucleic acids (PNAs) sequence information is obtained from the unimolecular decomposition of singly-charged PNA oligomers in the negative-ion mode using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry and sustained off-resonance irradiation collision induced dissociation.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2001 journal article
Detection of double-stranded PCR amplicons at the attomole level electrosprayed from low nanomolar solutions using FT-ICR mass spectrometry
Fresenius' Journal of Analytical Chemistry, 369(3-4), 246–251.
MeSH headings : Alleles; Cyclotrons; DNA / chemistry; DNA / genetics; Fourier Analysis; Homozygote; Humans; Mass Spectrometry / methods; Polymerase Chain Reaction / methods; Sensitivity and Specificity; Solutions; Spectrometry, Mass, Electrospray Ionization / methods; Spectrophotometry / methods
TL;DR:
A remarkable sensitivity for large PCR amplicons will dramatically improve the ability of electrospray ionization mass spectrometry to address important genetic questions for low copy number genes or when the amount of initial template is limited; the latter issue is commonly encountered in DNA forensics.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2001 journal article
Genotyping of Simple and Compound Short Tandem Repeat Loci Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Chemistry, 73(18), 4514–4521.
MeSH headings : Cyclotrons; Genotype; Humans; Microsatellite Repeats; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The utility of electrospray ionization Fourier transform ion cyclotron resonance (ESI-FIICR) mass spectrometry as a new approach for genotyping short tandem repeats (STRs) is demonstrated and is a powerful approach to characterize both simple and compound STRs beyond the capabilities of electrophoretic technologies.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2001 journal article
Genotyping short tandem repeats using flow injection and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 15(5), 348–350.
MeSH headings : Alleles; Cyclotrons; Flow Injection Analysis; Fourier Analysis; Genotype; Homozygote; Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Tandem Repeat Sequences
TL;DR:
Characterizing polymerase chain reaction (PCR) amplicons has been accomplished for the first time using flow injection analysis coupled to electrospray ionization mass spectrometry (ESI-MS) to illustrate the compatibility of ESI-MS for genotyping short tandem repeat sequences and the potential for high-throughput genotypesing of large PCR amplicons.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2001 journal article
High-Mass Accuracy of Product Ions Produced by SORI-CID Using a Dual Electrospray Ionization Source Coupled with FTICR Mass Spectrometry
Analytical Chemistry, 73(6), 1247–1251.
MeSH headings : Cyclotrons; Fourier Analysis; Oligonucleotides / analysis; Spectrometry, Mass, Electrospray Ionization
TL;DR:
This internal standardization method demonstrated significantly more consistent accurate mass measurements with average mass errors ranging from -1.2 to -3.2 ppm for the 15-mer oligonucleotide used in this study.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2001 journal article
Homogeneous Preparations of 3′-Phosphoglycolate-Terminated Oligodeoxynucleotides from Bleomycin-Treated DNA as Verified by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Biochemistry, 289(2), 274–280.
MeSH headings : Alkaline Phosphatase / pharmacology; Antimetabolites, Antineoplastic / pharmacology; Bleomycin / pharmacology; Chromatography, High Pressure Liquid; DNA / drug effects; DNA Damage / drug effects; DNA Repair; Electrophoresis, Polyacrylamide Gel; Glycolates / metabolism; Mass Spectrometry; Oligonucleotides / metabolism; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared; Time Factors
TL;DR:
Single- and double-strand breaks bearing 3'-phosphoglycolate termini are among the most frequent lesions formed in DNA by ionizing radiation and other oxidative mutagens and could be relabeled with polynucleotide kinase to yield highly purified, high-specific-activity substrates for repair studies.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2001 journal article
Impact of ion cloud densities on the measurement of relative ion abundances in Fourier transform ion cyclotron resonance mass spectrometry: experimental observations of coulombically induced cyclotron radius perturbations and ion cloud dephasing rates
Journal of Mass Spectrometry, 36(2), 195–203.
author keywords: space-charge; ion cloud density; signal decay; proteomics; quantification
MeSH headings : Cyclosporine / chemistry; Ions; Spectroscopy, Fourier Transform Infrared
TL;DR:
Experimental evidence supports that it is the loss of cloud coherence that accounts for the signal loss over time, with the less dense cloud de-phasing more quickly, as the ion populations of the two investigated species near equivalence, so do their time constants.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2001 journal article
Perspectives on the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for short tandem repeat genotyping in the post‐genome era
Journal of Mass Spectrometry (Special Feature), 36(6), 589–606.
author keywords: electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry; short tandem repeats; genotyping; DNA; human genome
MeSH headings : Alleles; Animals; Cyclotrons; Fourier Analysis; Genetic Markers; Genotype; Humans; Reverse Transcriptase Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Tandem Repeat Sequences / genetics
TL;DR:
This perspective is intended to familiarize the mass spectrometry community with modern genomics and to report on the current state of mass Spectrometry, specifically electrospray ionization Fourier transform ion cyclotron resonance mass spectromaetry, for characterization of STRs.
(via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: March 17, 2021
2001 journal article
Selective, Sensitive, and Rapid Phosphopeptide Identification in Enzymatic Digests Using ESI-FTICR-MS with Infrared Multiphoton Dissociation
Analytical Chemistry, 73(14), 3305–3311.
MeSH headings : Amino Acid Sequence; Animals; Caseins / analysis; Cattle; Fourier Analysis; Infrared Rays; Molecular Sequence Data; Phosphopeptides / analysis; Receptor, Insulin / analysis; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
The sensitivity and selectivity of phosphopeptides is shown to greatly improve when the volatile base piperidine is used to adjust the pH of the digestion of naturally phosphorylated proteins bovine alpha- and beta-casein.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2000 journal article
A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in Fourier transform ion cyclotron resonance mass spectrometry
Journal of the American Society for Mass Spectrometry, 11(10), 876–883.
MeSH headings : Biopolymers / chemistry; Calibration; Cyclotrons; Fourier Analysis; Mass Spectrometry; Reverse Transcriptase Polymerase Chain Reaction; Spectrophotometry, Ultraviolet
TL;DR:
An ESI source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry, illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2000 entry reference
Absolute Quantification of Proteins by Mass Spectrometry
TL;DR:
All phases of the PC-IDMS workflow, from the SIL peptide production platforms to data analysis, are described and characterized in detail and an emphasis is placed on the practical quantitative aspects for each step of the workflow and their implication on quantitative accuracy.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 18, 2021
2000 journal article
An experimental and theoretical study of the gas-phase decomposition of monoprotonated peptide nucleic acids
Journal of the American Society for Mass Spectrometry, 11(7), 615–625.
MeSH headings : Acetylation; Cyclotrons; Fourier Analysis; Gas Chromatography-Mass Spectrometry; Molecular Weight; Peptide Nucleic Acids / chemistry; Protons; Terminology as Topic
TL;DR:
Electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry shows great potential as a tool for the characterization and structural elucidation of these oligonucleotides.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2000 journal article
Preparation of single-stranded PCR products for electrospray ionization mass spectrometry using the DNA repair enzyme lambda exonuclease
The Analyst, 125(4), 619–626.
MeSH headings : Base Sequence; DNA, Single-Stranded; Exodeoxyribonucleases; Mass Spectrometry; Molecular Sequence Data; Polymerase Chain Reaction / methods; Tyrosine 3-Monooxygenase / genetics; Viral Proteins
TL;DR:
Lambda exonuclease clearly provides mass spectrometrists with a method to generate single-stranded PCR products and shows that one strand is preferentially adenylated over the other, which cannot be determined from the mass spectrum of the double-Stranded PCR product alone.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Accurate characterization of the tyrosine hydroxylase forensic allele 9.3 through development of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 13(10), 954–962.
TL;DR:
The results indicate that ESI-FTICR-MS is a powerful approach to rapidly and accurately characterize tandem repeating sequences which will ultimately lead towards the understanding of a complex class of diseases and in human identity determination.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Characterization of a microdialysis approach to prepare polymerase chain reaction products for electrospray ionization mass spectrometry using on-line ultraviolet absorbance measurements and inductively coupled plasma-atomic emission spectroscopy
Rapid Communications in Mass Spectrometry, 13(5), 323–330.
TL;DR:
The desalting efficiencies for microdialysis have been quantitatively determined using inductively coupled plasma atomic emission spectroscopy (ICP-AES) by analyzing samples which mimic polymerase chain reaction (PCR) conditions and revealed an insensitivity to molecular weight over a broad range.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Consequences of Nucleic Acid Conformation on the Binding of a Trinuclear Platinum Drug†
Biochemistry, 38(45), 14731–14737.
MeSH headings : Antineoplastic Agents / metabolism; DNA / metabolism; DNA, Single-Stranded / metabolism; Mass Spectrometry; Nucleic Acid Conformation; Organoplatinum Compounds / metabolism; RNA / metabolism; Spectroscopy, Fourier Transform Infrared; Structure-Activity Relationship
TL;DR:
BBR3464 binding to different nucleic acid conformations raises the possibility that the adducts of single-stranded DNA and RNA may play a role in the different antitumor efficacies of this novel drug as compared with cisplatin.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Hemoglobinase Activity of the Lysine Gingipain Protease (Kgp) of Porphyromonas gingivalis W83
Journal of Bacteriology, 181(16), 4905–4913.
MeSH headings : Adhesins, Bacterial; Agar; Amino Acid Sequence; Blood; Chromatography; Chromosome Mapping; Cysteine Endopeptidases / genetics; Cysteine Endopeptidases / isolation & purification; Cysteine Endopeptidases / metabolism; Enzyme Activation / drug effects; Enzyme Inhibitors / pharmacology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Gingipain Cysteine Endopeptidases; Helminth Proteins; Hemagglutinins / genetics; Hemagglutinins / isolation & purification; Hemagglutinins / metabolism; Heme / metabolism; Hemin / pharmacology; Hemoglobins / analysis; Hemoglobins / chemistry; Hemolysis; Humans; Imidazoles / pharmacology; Iron / metabolism; Mass Spectrometry; Membrane Proteins / metabolism; Molecular Sequence Data; Mutagenesis; Peptide Fragments / analysis; Peptide Fragments / chemistry; Porphyromonas gingivalis / enzymology; Porphyromonas gingivalis / genetics; Porphyromonas gingivalis / growth & development
TL;DR:
The ability of the Lys-gingipain to cleave human hemoglobin at Lys residues was confirmed by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of hemoglobin fragments resulting from digestion with the purified protease, and several of the predicted Hemoglobin fragments rendered by digestion with purified Lys-gesipain were detected.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Hydropathic influences on the quantification of equine heart cytochromec using relative ion abundance measurements by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Journal of Mass Spectrometry, 34(10), 1055–1062.
author keywords: proteomics; quantification; hydropathy; electrospray ionization; Fourier transform ion cyclotron resonance mass spectrometry
TL;DR:
The results present significant considerations when choosing internal standards for the quantification of large proteins by ESI-FTICR-MS and demonstrate that relative molecular ion signals in FTICR can be used to quantify macromolecular species in the nanomolar regime.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Precise mass measurement of a double-stranded 500 base-pair (309 kDa) polymerase chain reaction product by negative ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 13(12), 1201–1204.
TL;DR:
It is demonstrated that precise mass measurements of PCR products up to 309 kDa (500 bp) can be routinely obtained by ESI-FTICR requiring low femtomole amounts.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1999 journal article
Quantification of singly charged biomolecules by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry utilizing an internal standard
Rapid Communications in Mass Spectrometry, 13(3), 164–171.
Sources: Crossref, ORCID
Added: February 27, 2021
1998 journal article
Comprehensive nomenclature for the fragment ions produced from collisional activation of peptide nucleic acids
Rapid Communications in Mass Spectrometry, 12(12), 759–762.
TL;DR:
It is becoming increasingly evident that multi-stage MS is especially suited to structural characterization of large bio-molecules such as PNA’s, and herein it is proposed a comprehensive nomenclature for the product ions produced upon collisional activation of PNA's, which is based upon extensive experimental studies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
1998 journal article
Heterogeneity inBacillus cereusPCR Products Detected by ESI−FTICR Mass Spectrometry
Analytical Chemistry, 70(6), 1203–1207.
MeSH headings : Bacillus cereus / genetics; Cyclotrons; DNA, Bacterial / analysis; Fourier Analysis; Mass Spectrometry / methods; Polymerase Chain Reaction
TL;DR:
It is illustrated that PCR products amplified from templates differing by a single nucleotide can be resolved and identified using ESI-FTICR at the 89-bp level and indicated the presence of an unreported sequence variation between rRNA operons in this organism.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1998 journal article
Mass Spectrometric Characterization of DNA for Molecular Biology Applications: Advances using MALDI and ESI
Advances in Mass Spectrometry, 14, 377–406.
Source: NC State University Libraries
Added: March 17, 2021
1998 journal article
Nanoelectrospray mass spectrometry using non-metalized, tapered (50 → 10 μm) fused-silica capillaries
Rapid Communications in Mass Spectrometry, 12(8), 443–448.
Sources: Crossref, ORCID
Added: February 12, 2021
1998 journal article
Sequencing and Characterization of Larger Oligonucleotides by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Reviews in Analytical Chemistry, 17(1), 1–68.
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Application of sequential paired covariance to liquid chromatography-mass spectrometry data enhancements in both the signal-to-noise ratio and the resolution of analyte peaks in the chromatogram
Journal of Chromatography A, 771(1-2), 1–7.
author keywords: sequential paired covariance; chemometrics; signal-to-noise ratio; resolution
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Improving the Microdialysis Procedure for Electrospray Ionization Mass Spectrometry of Biological Samples
Journal of Mass Spectrometry, 32(4), 425–431.
author keywords: microdialysis; desalting; electrospray ionization mass spectrometry; DNA
TL;DR:
Significant advances in the area of microdialysis which allowed more effective handling of small volumes (microliters) of samples, more efficient desalting and enhanced mass spectrometric detection sensitivity are described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Length and Base Composition of PCR-Amplified Nucleic Acids Using Mass Measurements from Electrospray Ionization Mass Spectrometry
Analytical Chemistry, 69(8), 1543–1549.
MeSH headings : Algorithms; Mass Spectrometry; Molecular Weight; Nucleic Acids / analysis; Nucleotides / analysis; Polymerase Chain Reaction
TL;DR:
It is shown that the use of modified bases in PCR or post-PCR modification chemistry allows unique solutions for the base composition of the PCR product with only modest mass measurement precision.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Instrumentation and Applications
Journal of Chemical Education, 74(11), 1288.
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Molecular Architecture via Coordination: Marriage of Crown Ethers and Calixarenes with Molecular Squares, Unique Tetranuclear Metallamacrocycles from Metallacrown Ether and Metallacalixarene Complexes via Self-Assembly
Journal of the American Chemical Society, 119(22), 5163–5168.
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Nanoscale Tectonics: Self-Assembly, Characterization, and Chemistry of a Novel Class of Organoplatinum Square Macrocycles
Journal of the American Chemical Society, 119(48), 11611–11619.
Sources: Crossref, ORCID
Added: February 12, 2021
1997 journal article
Transition-Metal-Mediated Rational Design and Self-Assembly of Chiral, Nanoscale Supramolecular Polyhedra with UniqueTSymmetry†
Organometallics, 16(14), 3094–3096.
Sources: Crossref, ORCID
Added: February 12, 2021
1996 journal article
A Quantitative Study ofin vitroHepatic Metabolism of Tacrolimus (FK506) Using Secondary Ion and Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
Rapid Communications in Mass Spectrometry, 10(10), 1215–1218.
TL;DR:
The identification and simultaneous quantification of Tacrolimus and its hepatic metabolites in baboons has been achieved using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry and static secondary-ion mass spectromaetry and TOF-SIMS.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1996 journal article
Analysis of Double-stranded Polymerase Chain Reaction Products from the Bacillus cereus Group by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Rapid Communications in Mass Spectrometry, 10(1), 29–35.
Source: NC State University Libraries
Added: March 8, 2021
1996 journal article
Characterization of PCR Products from Bacilli Using Electrospray Ionization FTICR Mass Spectrometry
Analytical Chemistry, 68(21), 3705–3712.
MeSH headings : Bacillus / genetics; Mass Spectrometry; Polymerase Chain Reaction; Spectroscopy, Fourier Transform Infrared
TL;DR:
This constitutes the first report demonstrating the ionization and detection of PCR products by mass spectrometry with mass precision and accuracy for assignment of such modifications or substitutions.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
1996 journal article
Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry
Journal of the American Society for Mass Spectrometry, 7(8), 697–706.
TL;DR:
Several oligonucleotides that varied in base composition and length were investigated, and studies of mixtures showed a significant reduction in spectral complexity.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1996 journal article
Design and Self-Assembly of Nanoscale Organoplatinum Macrocycles
Journal of the American Chemical Society, 118(36), 8731–8732.
Sources: Crossref, ORCID
Added: February 12, 2021
1996 journal article
Direct quantification of cocaine in urine by time-of-flight mass spectrometry
Fresenius' Journal of Analytical Chemistry, 354(1), 103–110.
TL;DR:
The direct quantification of cocaine in urine has been achieved using time-of-flight secondary-ion mass spectrometry (TOF-SIMS) using a dynamic range of four orders of magnitude using one internal standard concentration.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
1996 journal article
Enhancement of ion intensity in time-of-flight secondary-ionization mass spectrometry
Journal of the American Society for Mass Spectrometry, 7(5), 467–472.
TL;DR:
A sample preparation technique is discovered that enhanced the SI yield of cyclosporin A (CsA) in an allograft patient sample and neat samples of CsA and polystyrene when deposited from neat solutions.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
1996 journal article
Important Aspects concerning the Quantification of Biomolecules by Time-of-Flight Secondary-Ion Mass Spectrometry
Applied Spectroscopy, 50(2), 161–166.
author keywords: SIMS; quantification; mass spectrometry; TOF-SIMS
Sources: Crossref, ORCID
Added: February 12, 2021
1995 journal article
Application of Sequential Paired Covariance to Capillary Electrophoresis Electrospray Ionization Time-of-Flight Mass Spectrometry: Unraveling the Signal from the Noise in the Electropherogram
Analytical Chemistry, 67(23), 4371–4375.
Sources: Crossref, ORCID
Added: February 12, 2021
1995 journal article
Application of secondary ion and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the quantitative analysis of biological molecules
Mass Spectrometry Reviews, 14(6), 383–429.
Sources: Crossref, ORCID
Added: February 12, 2021
1995 journal article
Simultaneous quantification of cyclosporin A and its major metabolites by time-of-flight secondary-ion mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry utilizing data analysis techniques: Comparison with high-performance liquid chromatography
Journal of Mass Spectrometry, 30(10), 1469–1479.
Sources: Crossref, ORCID
Added: February 27, 2021
1994 journal article
Characterization of Polystyrene on Etched Silver Using Ion Scattering and X-ray Photoelectron Spectroscopy: Correlation of Secondary Ion Yield in Time-of-Flight SIMS with Surface Coverage
The Journal of Physical Chemistry, 98(44), 11570–11575.
Sources: Crossref, ORCID
Added: February 12, 2021
1994 journal article
Quantitative Measurement of Cyclosporin A in Blood by Time-of-Flight Mass Spectrometry
Analytical Chemistry, 66(14), 2362–2368.
MeSH headings : Chromatography, High Pressure Liquid; Cyclosporine / blood; Fluorescence Polarization Immunoassay; Humans; Mass Spectrometry; Spectrometry, Mass, Fast Atom Bombardment
TL;DR:
Blood samples were analyzed by a nonspecific method, fluorescence polarization immunoassay (FPIA), and by a specific method, high-performance liquid chromatography (HPLC), and the TOF-MS results are in good agreement with the HPLC results, but not with the FPIA results.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
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