@article{taliercio_eickholt_read_carter_waldeck_fallen_2023, title={Parental choice and seed size impact the uprightness of progeny from interspecific Glycine hybridizations}, ISSN={["1435-0653"]}, DOI={10.1002/csc2.21015}, abstractNote={Abstract}, journal={CROP SCIENCE}, author={Taliercio, Earl and Eickholt, David and Read, Quentin D. and Carter, Thomas and Waldeck, Nathan and Fallen, Ben}, year={2023}, month={Jun} }
 @article{eickholt_carter_taliercio_dickey_dean_delheimer_li_2019, title={Registration of USDA-Max x Soja Core Set-1: Recovering 99% of Wild Soybean Genome from PI 366122 in 17 Agronomic Interspecific Germplasm Lines}, volume={13}, ISSN={["1940-3496"]}, DOI={10.3198/jpr2017.09.0059crg}, abstractNote={USDA‐Max × Soja Core Set‐1 (USDA‐MxS‐CS1‐1 to USDA‐MxS‐CS1‐17 [Reg. No. GP‐417 to GP‐433, PI 689053 to PI 689069]) is a group of 17 interspecific breeding lines developed from the hybridization of lodging‐resistant soybean cultivar N7103 [Glycine max (L.) Merr.] with wild soybean plant introduction PI 366122 [G. soja Siebold & Zucc.]. These materials were released by the USDA‐ARS and the North Carolina Agricultural Research Service (March 2017) to expand the North American soybean breeding pool. The full‐sib breeding lines are 50% wild soybean by pedigree and developed through bulk breeding and pedigree selection. Marker analysis of 2455 well‐distributed polymorphic single‐nucleotide polymorphism loci revealed that individual breeding lines ranged from 21 to 40% alleles derived from wild soybean. Collectively, most of the wild soybean genome was transferred to the core set in that 5, 10, and 17 breeding lines captured 83, 98, and 99% of G. soja–derived polymorphic alleles. Physical linkage maps suggested that extensive recombination occurred between the G. max and G. soja genomes. The 17 breeding lines are well adapted to the southeastern United States, exhibited seed yield ranging from 75 to 97% of the domesticated parent, and are group VI or VII maturity. Some breeding lines displayed increased seed protein, oil, or methionine content, and all exhibited increased seed size as compared to the domesticated parent. The novel genetic diversity, positive agronomic performance, and improved seed composition of these lines suggest that they are valuable genetic resources for US soybean breeding.}, number={2}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Eickholt, David and Carter, Thomas E., Jr. and Taliercio, Earl and Dickey, David and Dean, Lisa O. and Delheimer, Jake and Li, Zenglu}, year={2019}, month={May}, pages={217–236} }
 @article{steede_ma_eickholt_drake-stowe_kernodle_shew_danehower_lewis_2017, title={The Tobacco Trichome Exudate Z-abienol and Its Relationship With Plant Resistance to Phytophthora nicotianae}, volume={101}, ISSN={["1943-7692"]}, DOI={10.1094/pdis-10-16-1512-re}, abstractNote={ In previous research, we discovered a favorable quantitative trait locus (QTL) in cigar tobacco cultivar ‘Beinhart 1000’ designated as Phn15.1, which provides a high level of partial resistance to the black shank disease caused by Phytophthora nicotianae. A very close genetic association was also found between Phn15.1 and the ability to biosynthesize Z-abienol, a labdanoid diterpene exuded by the trichomes onto above-ground plant parts, and that imparts flavor and aroma characteristics to Oriental and some cigar tobacco types. Because accumulation of Z-abienol is considered to be undesirable for cultivars of other tobacco types, we herein describe a series of experiments to gain insight on whether this close association is due to genetic linkage or pleiotropy. First, in an in vitro bioassay, we observed Z-abienol and related diterpenes to inhibit hyphal growth of P. nicotianae at concentrations between 0.01 and 100 ppm. Secondly, we field-tested transgenic versions of Beinhart 1000 carrying RNAi constructs for downregulating NtCPS2 or NtABS, two genes involved in the biosynthesis of Z-abienol. Thirdly, we also field tested a recombinant inbred line population segregating for a truncation mutation in NtCPS2 leading to an interrupted Z-abienol pathway. We observed no correlation between field resistance to P. nicotianae and the ability to accumulate Z-abienol in either the transgenic materials or the mapping population. Results suggest that, although Z-abienol may affect P. nicotianae when applied at high concentrations in in vitro assays, the compound has little effect on black shank disease development under natural field conditions. Thus, it should be possible to disassociate Phn15.1-mediated black shank resistance identified in cigar tobacco cultivar Beinhart 1000 from the ability to accumulate Z-abienol, an undesirable secondary metabolite for burley and flue-cured tobacco cultivars. }, number={7}, journal={PLANT DISEASE}, author={Steede, William T. and Ma, Justin M. and Eickholt, David P. and Drake-Stowe, Katherine E. and Kernodle, Sheri P. and Shew, H. David and Danehower, David A. and Lewis, Ramsey S.}, year={2017}, month={Jul}, pages={1214–1221} }
 @article{wells_eickholt_lewis_vann_fisher_2016, title={Heat Unit Accumulation and Days to Anthesis Relationship in Tobacco Genotypes with an Introgressed QTL Affecting Leaf Number}, volume={56}, ISSN={["1435-0653"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84990194504&partnerID=MN8TOARS}, DOI={10.2135/cropsci2016.04.0278}, abstractNote={Plant breeders and crop managers would benefit from an increased ability to predict the requirements for onset of anthesis in tobacco (Nicotiana tabacum L.). This study was performed to determine the relationship in the field between heat unit (HU) accumulation and the onset of anthesis in tobacco genotypes varying for the zygosity of an introgressed quantitative trait loci (QTL) designated as Many Leaves (Ml) affecting flowering team and leaf number. Three commercially important cultivars or breeding lines (Speight 168, K326, and NCTG‐61), their nearly isogenic homozygous (MlMl) and heterozygous (Mlml) counterparts, and three BC6F3 null‐segregant (mlml) lines, were evaluated at three locations and over 2 yr. Days to anthesis (DTA) and HU accumulation were determined. In each environment, the MlMl, Mlml, and mlml genotypes were found to be grouped according to the zygosity of the Ml trait, with the mlml genotypes flowering first followed by Mlml and MlMl genotypes, respectively. An exception to this trend was Speight 168 MlMl, which consistently fell within the Mlml grouping for DTA. No consistent relationships were observed between HU and DTA, which was highly variable among environments. Integrated HU (area under the HU curve) during the 28 d after transplanting (DAT) was positively associated (R2 = 0.98; P = 0.0001) with the range of DTA among all genotypes. Integrated HU was a better indicator of the pattern of HU attainment than merely the amount. Early HU exposure seemed important in determining the range in DTA amongst the genotypes examined.}, number={6}, journal={CROP SCIENCE}, author={Wells, Randy and Eickholt, David P. J. and Lewis, Ramsey and Vann, Matthew C. and Fisher, Loren R.}, year={2016}, pages={3228–3236} }
 @article{eickholt_lewis_2014, title={Effect of an Introgressed Nicotiana tomentosa Leaf Number QTL on Yield and Quality Characteristics in Flue-Cured Tobacco}, volume={54}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.07.0464}, abstractNote={ABSTRACT}, number={2}, journal={CROP SCIENCE}, author={Eickholt, David P. and Lewis, Ramsey S.}, year={2014}, pages={586–594} }
 @article{eickholt_lewis_2013, title={Breeding Cycles Expedited by FT-mediated Reduction in Generation Time}, volume={53}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.03.0150}, abstractNote={ABSTRACT}, number={6}, journal={CROP SCIENCE}, author={Eickholt, David P. and Lewis, Ramsey S.}, year={2013}, pages={2384–2391} }