@article{wang_de los reyes iii_ducoste_2023, title={Microplate-Based Cell Viability Assay as a Cost-Effective Alternative to Flow Cytometry for Microalgae Analysis}, volume={57}, ISSN={["1520-5851"]}, url={https://doi.org/10.1021/acs.est.3c05675}, DOI={10.1021/acs.est.3c05675}, abstractNote={Cell viability is a critical indicator for assessing culture quality in microalgae cultivation for biorefinery and bioremediation. Fluorescent dyes that distinguish viable from nonviable cells can enable viability quantification based on the percentage of live cells. However, fluorescence analysis using the typical flow cytometry method is costly and impractical for industrial applications. To address this, we developed new microplate assays utilizing fluorescein diacetate as a live cell stain and erythrosine B as a dead cell stain. These assays provide a low-cost, simple, and reliable method of assessing cell viability. The proposed microplate assays were successfully applied to monitor the viability of the microalgae Dunaliella viridis under carbon and nitrogen limitation stresses and demonstrated good agreement with flow cytometry measurements. We conducted a systematic investigation of the effects of dye concentration, incubation time, and background fluorescence on the microplate assays' performance. Further, we provide a comprehensive review of commonly used fluorescent dyes for microalgae staining, discuss strategies to enhance assay performance, and offer recommendations for dye selection and protocol development. This study presents a comprehensive new method for microplate-based viability analysis, providing valuable insights for future microalgae viability assessments and applications.}, number={50}, journal={ENVIRONMENTAL SCIENCE & TECHNOLOGY}, author={Wang, Diyuan and de los Reyes III, Francis L. and Ducoste, Joel J.}, year={2023}, month={Dec}, pages={21200–21211} }
 @article{bernardy_elardo_trautz_malley_wang_ducoste_2022, title={Effects of UV-C Disinfection on N95 and KN95 Filtering Facepiece Respirator Reuse}, volume={9}, ISSN={["1098-5336"]}, DOI={10.1128/aem.01221-22}, abstractNote={
            Research suggests degradation of FFR materials at high UV doses is important. There appears to be a peak inactivation dose at approximately 1,500 mJ/cm
            2
            . The subsequent dose increases appear to have the reverse effect on inactivation values; these trends have shown true with both the N95 and KN95-Purism respirators.
          }, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Bernardy, Castine and Elardo, Nicola and Trautz, Alexa and Malley, Jim and Wang, Diyuan and Ducoste, Joel}, year={2022}, month={Sep} }