Drake Phelps
zebrafish, immunology, toxicology, immunotoxicology, PFAS
I'm an immunotoxicologist who is passionate about protecting human and environmental health from toxic threats, such as EDCs and PFAS.
Works (11)
Updated: May 25th, 2024 05:00
2024 journal article
Per- and polyfluoroalkyl substances alter innate immune function: evidence and data gaps
Journal of Immunotoxicology, 21(1).
Contributors: n , A. Connors n , G. Ferrero n, J. DeWitt n & J. Yoder n
author keywords: Per- and polyfluoroalkyl substances; PFAS; innate immunity; ecotoxicology; zebrafish; immunotoxicology
www.tandfonline.com (publisher PDF)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: May 8, 2024
2023 journal article
Legacy and emerging per- and polyfluoroalkyl substances suppress the neutrophil respiratory burst
JOURNAL OF IMMUNOTOXICOLOGY, 20(1).
author keywords: Innate immunity; neutrophils; per- and polyfluoroalkyl substances (PFAS); reactive oxygen species; zebrafish
MeSH headings : Animals; Humans; Zebrafish; Neutrophils; Reactive Oxygen Species; Respiratory Burst; Fluorocarbons / toxicity; Alkanesulfonic Acids / toxicity; Environmental Pollutants
TL;DR:
This report is the first to demonstrate that these PFASs suppress neutrophil function and support the utility of employing zebrafish larvae and a human cell line as screening tools to identify chemicals that may suppress human immune function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 15, 2023
2021 journal article
Monoassociation with bacterial isolates reveals the role of colonization, community complexity and abundance on locomotor behavior in larval zebrafish
Animal Microbiome, 3(1).
author keywords: Microbiome; Hyperactivity; Monoassociation; Gnotobiotic; Germ-free; Axenic; Zebrafish; Monocolonization
TL;DR:
The results suggest that differences in the composition of microbes in fish facilities could affect the results of behavioral assays within pharmacological and toxicological studies, and support a growing body of evidence that individual species of bacteria can have different effects on host behavior.
(via Semantic Scholar)
Source: ORCID
Added: May 8, 2021
2020 journal article
Derivation of stable embryonic stem cell-like, but transcriptionally heterogenous, induced pluripotent stem cells from non-permissive mouse strains
MAMMALIAN GENOME, 31(9-12), 263–286.
MeSH headings : Animals; Biomarkers; Cell Differentiation; Cells, Cultured; Cellular Reprogramming / genetics; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Gene Expression Profiling; Gene Expression Regulation, Developmental; Genetic Heterogeneity; Immunophenotyping; Induced Pluripotent Stem Cells / cytology; Induced Pluripotent Stem Cells / metabolism; Mice; Platelet Endothelial Cell Adhesion Molecule-1; Species Specificity; Transcriptome
TL;DR:
Heterogeneity is a naturally occurring, necessary quality of stem cells that allows for the maintenance of pluripotency and this study is the first study to derive stable ESC-like stem cells from the non-permissive NOD/ShiLtJ and WSB/EiJ strains, enabling easier and broader research possibilities into pluripOTency for these and similar non-Permissive mouse strains and species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 19, 2020
2020 journal article
In vivo assessment of respiratory burst inhibition by xenobiotic exposure using larval zebrafish
Journal of Immunotoxicology, 17(1), 94–104.
author keywords: Chemical screen; high throughput; phagocyte; reactive oxygen species (ROS); polycyclic aromatic hydrocarbons (PAH); endocrine disrupting compounds (EDC); lead
MeSH headings : Animals; Animals, Genetically Modified; Benzo(a)pyrene / adverse effects; Blood Cell Count; Cytotoxicity Tests, Immunologic / methods; Embryo, Nonmammalian; Estradiol / adverse effects; Feasibility Studies; High-Throughput Screening Assays / methods; Immunity, Innate / drug effects; Macrophages / drug effects; Macrophages / immunology; Methoxychlor / adverse effects; Neutrophils / drug effects; Neutrophils / immunology; Organometallic Compounds / adverse effects; Phenanthrenes / adverse effects; Reactive Oxygen Species / analysis; Reactive Oxygen Species / metabolism; Respiratory Burst / drug effects; Respiratory Burst / immunology; Zebrafish
TL;DR:
The utility of zebrafish larvae as a vertebrate model for identifying compounds that impact innate immune function at non-teratogenic levels is demonstrated and validates measuring ROS production and phagocyte numbers as metrics for monitoring how xenobiotic exposure alters the innate immune system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: May 15, 2020
2019 journal article
Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17β-estradiol
Scientific Reports, 9(1).
MeSH headings : Animals; Embryonic Development / drug effects; Estradiol / metabolism; Estradiol / pharmacology; Estrogens / metabolism; Estrogens / pharmacology; Germ-Free Life / drug effects; Larva / drug effects; Larva / metabolism; Locomotion / drug effects; Microbiota / drug effects; Microbiota / genetics; Neurogenesis / drug effects; RNA, Ribosomal, 16S / genetics; Zebrafish / embryology; Zebrafish / metabolism; Zebrafish / microbiology
TL;DR:
It is demonstrated that E2 locomotor effects in the light phase are dependent on the presence of microbiota and suggest that microbiota influence chemical E2 toxicokinetics, and the concept that microbial colonization status may influence chemical toxicity is supported.
(via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: September 23, 2019
2019 journal article
Triclosan-selected host-associated microbiota perform xenobiotic biotransformations in larval zebrafish
Toxicological Sciences.
author keywords: microbiome; microbiota; zebrafish; triclosan; triclosan sulfate; toxicokinetics; antimicrobial; antibacterial
TL;DR:
Data support the concept that microbiota modify the toxicokinetics of xenobiotic exposure and suggest that triclosan exposure selects for microbes that harbor the capacity to biotransform tricrosan into chemical metabolites with unknown toxicity profiles.
(via Semantic Scholar)
Source: ORCID
Added: September 23, 2019
2018 journal article
Host developmental toxicity of BPA and BPA alternatives is inversely related to microbiota disruption in zebrafish
Toxicological Sciences, 10.
author keywords: microbiome; zebrafish; developmental toxicity; BPA
MeSH headings : Animals; Behavior, Animal / drug effects; Benzhydryl Compounds / chemistry; Benzhydryl Compounds / toxicity; Dose-Response Relationship, Drug; Environmental Pollutants / chemistry; Environmental Pollutants / toxicity; Larva / drug effects; Larva / microbiology; Microbiota / drug effects; Microbiota / genetics; Phenols / chemistry; Phenols / toxicity; RNA, Ribosomal, 16S; Structure-Activity Relationship; Zebrafish / growth & development; Zebrafish / microbiology
TL;DR:
It is indicated that some BP analogs disrupt host microbiota early in life and demonstrate novel chemical-microbiota interactions that may add important context to current hazard identification strategies.
(via Semantic Scholar)
Source: ORCID
Added: September 23, 2019
2018 journal article
Permissiveness to form pluripotent stem cells may be an evolutionarily derived characteristic in Mus muscuius
SCIENTIFIC REPORTS, 8.
MeSH headings : Animals; Cells, Cultured; Cellular Reprogramming / drug effects; Cellular Reprogramming / genetics; Culture Media / metabolism; Culture Media / pharmacology; Evolution, Molecular; Female; Fibroblasts; Glycogen Synthase Kinase 3 beta / antagonists & inhibitors; Glycogen Synthase Kinase 3 beta / metabolism; Induced Pluripotent Stem Cells; Leukemia Inhibitory Factor / metabolism; MAP Kinase Kinase Kinases / antagonists & inhibitors; MAP Kinase Kinase Kinases / metabolism; Male; Mice / genetics; Mice, Inbred Strains / genetics; Mouse Embryonic Stem Cells; Primary Cell Culture; Protein Kinase Inhibitors / pharmacology; Sex Factors; Transcription, Genetic
TL;DR:
iPSC derived in 2iS are more transcriptionally similar to ESC than EpiSC, indicating that iPSC reprogramming acts to overcome genetic background constraints, and only some M. musculus strains are permissive under LIF culture conditions suggesting that this is an evolutionarily derived characteristic in the M.Mus musculus lineage.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 16, 2018
2017 journal article
Development, and genetic and metabolic characterization of new tomato mutants with enhanced and deficient carotenoid content
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 92(5), 475–483.
author keywords: Carotenoid; lycopene; -carotene; Solanum lycopersicum; mutagenesis
TL;DR:
Genetic and metabolic analyses showed that new mutants with enhanced and deficient carotenoid content in a single genetic background of tomato cv.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Microbial colonization is required for normal neurobehavioral development in zebrafish
Scientific Reports.
MeSH headings : Aeromonas veronii / growth & development; Animals; Anti-Bacterial Agents / administration & dosage; Behavior, Animal; Embryo, Nonmammalian; Gastrointestinal Microbiome; Larva / growth & development; Larva / microbiology; Locomotion; Nervous System / growth & development; Vibrio cholerae / growth & development; Zebrafish / growth & development; Zebrafish / microbiology
TL;DR:
Data show that microbial colonization during early life is required for normal neuro behavioral development and support the concept that antibiotics and other environmental chemicals may exert neurobehavioral effects via disruption of host-associated microbial communities.
(via Semantic Scholar)
Source: ORCID
Added: September 23, 2019
Employment
Updated: March 6th, 2024 18:14
2023 - present
ORISE/USEPA Durham, North Carolina, US
ORISE Postdoctoral Fellow at USEPA
USEPA/ORD/CCTE/BCTD/RADB
2023 - 2023
East Carolina University Greenville, North Carolina, US
Postdoctoral Scholar
Brody School of Medicine: Department of Pharmacology and Toxicology
2022 - 2023
Food Packaging Forum Zurich, Zürich, CH
Independent Consultant
2022 - 2022
NC State University Raleigh, NC, US
Postdoctoral Research Scholar
Molecular Biomedical Sciences
2017 - 2022
NC State University Raleigh, NC, US
Graduate Research Assistant
Molecular Biomedical Sciences
2019 - 2019
Center for Environmental Health Durham, NC, US
Graduate Intern
2016 - 2017
ORISE/USEPA Durham, NC, US
ORISE Postbaccalaureate Fellow at USEPA
USEPA/ORD/NHEERL/ISTD/SBB
2015 - 2015
North Carolina State University Raleigh, NC, US
Undergraduate Research Assistant
Prestage Department of Poultry Science
2014 - 2015
North Carolina State University Raleigh, NC, US
Undergraduate Research Assistant
Program of Genetics
2011 - 2012
Institute for Sustainable and Renewable Resources Danville, VA, US
Undergraduate Research Assistant
Education
Updated: June 8th, 2022 15:12
2017 - 2022
NC State University Raleigh, NC, US
PhD in Comparative Biomedical Sciences
2012 - 2015
North Carolina State University Raleigh, NC, US
Bachelor of Science in Microbiology with Minors in Biotechnology and French Language
2010 - 2012
Danville Community College Danville, VA, US
Associate of Arts and Sciences
Funding History
Funding history based on the linked ORCID record. Updated: December 18th, 2019 10:30
grant
July 1, 2018 - July 1, 2020
NIH/NCSU Molecular Biology Training Program
National Institutes of Health
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