Emily Ozpinar
Also known as: Emily A. Wrona
Works (20)
Updated: August 21st, 2023 21:15
2021 journal article
Monitoring decellularization via absorbance spectroscopy during the derivation of extracellular matrix scaffolds
BIOMEDICAL MATERIALS, 17(1).
author keywords: extracellular matrix; manufacturing; decellularization; monitoring; vocal fold
MeSH headings : Biocompatible Materials; Extracellular Matrix / chemistry; Hydrogels; Regenerative Medicine; Spectrum Analysis; Tissue Engineering / methods; Tissue Scaffolds
TL;DR:
Monitoring the bioreactor’s effluent absorbance at 260 nm as a function of time will provide a representative DNA release profile from the tissue and thus allow for process optimization, showing that absorbance profiles are a good representation of DNA removal during the decellularization process thus providing an important tool to optimize future protocols.
(via Semantic Scholar)
open access via iopscience.iop.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: November 4, 2021
2021 journal article
Transcriptome-targeted analysis of human peripheral blood-derived macrophages when cultured on biomaterial meshes
BIOMEDICAL MATERIALS, 16(2).
author keywords: surgical meshes; macrophage polarization; biomaterials; targeted transcriptome; poly-4-hydroxybutyrate (P4HB); polypropylene
MeSH headings : Anti-Inflammatory Agents / chemistry; Biocompatible Materials / chemistry; Cell Culture Techniques; Cell Differentiation; Cell Separation; Cells, Cultured; Flow Cytometry; Gene Expression Profiling; Humans; Hydroxybutyrates; Immunity, Innate; In Vitro Techniques; Inflammation; Macrophages / drug effects; Macrophages / metabolism; Materials Testing; Monocytes / cytology; Phenotype; Polypropylenes / chemistry; Prostheses and Implants; RNA / metabolism; Surgical Mesh; Tissue Scaffolds; Transcriptome; Treatment Outcome; Up-Regulation; Wound Healing
TL;DR:
In vitro evidence is provided for the early transcriptomic targeted signature of human Mφs upon two commonly used surgical meshes and suggests a transition from an inflammatory to a non-inflammatory phenotype by P4HB as well as an upregulation of genes annotated under the pathogen response pathway.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 16, 2021
2020 journal article
An off-the-shelf artificial cardiac patch improves cardiac repair after myocardial infarction in rats and pigs
Science Translational Medicine, 12(538).
MeSH headings : Animals; Cell- and Tissue-Based Therapy; Extracellular Matrix; Heart; Myocardial Infarction / therapy; Myocardium; Rats; Swine
TL;DR:
The artCP is a clinically feasible, easy-to-store, and cell-free alternative to myocardial repair using cell-based cardiac patches and supported cardiac recovery by reducing scarring, promoting angiomyogenesis, and boosting cardiac function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: April 9, 2020
2020 journal article
Dermal Extracellular Matrix-Derived Hydrogels as an In Vitro Substrate to Study Mast Cell Maturation
TISSUE ENGINEERING PART A, 27(15-16), 1008–1022.
Contributors: n , A. Frey n, G. Arthur n, C. Mora-Navarro n, A. Biehl n, D. Snider n, G. Cruse n , D. Freytes n
author keywords: extracellular matrix; mast cell; allergy; metabolism
MeSH headings : Animals; Cell Differentiation; Collagen; Extracellular Matrix; Humans; Hydrogels; Mast Cells; Swine
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 11, 2020
2020 journal article
Dermal Extracellular Matrix-Derived Hydrogels as an In Vitro Substrate to Study Mast Cell Maturation
Tissue Engineering Part A.
MeSH headings : Animals; Cell Differentiation; Collagen; Extracellular Matrix; Humans; Hydrogels; Mast Cells; Swine
Source: ORCID
Added: October 4, 2020
2020 journal article
Fast Automated Approach for the Derivation of Acellular Extracellular Matrix Scaffolds from Porcine Soft Tissues
ACS Biomaterials Science & Engineering, 6(7), 4200–4213.
author keywords: automation; decellularization; extracellular matrix; hydrogel; biomaterial; biofabrication
MeSH headings : Animals; Biocompatible Materials; Bioreactors; Extracellular Matrix; Hydrogels; Swine; Tissue Scaffolds
TL;DR:
This work presents a versatile, scalable, and automated platform for the rapid production of ECM scaffolds with a biofabrication method for fast and automated derivation of raw material for ECM hydrogel production while preserving ECM composition and controlling lot-to-lot variability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: June 16, 2020
2020 journal article
Mast Cell-Biomaterial Interactions and Tissue Repair
TISSUE ENGINEERING PART B-REVIEWS, 27(6), 590–603.
Contributors: , A. Frey n, G. Cruse n & D. Freytes n
author keywords: mast cells; biomaterials; inflammation; tissue repair; foreign body response; wound healing
MeSH headings : Biocompatible Materials; Cell Communication; Fibrosis; Humans; Mast Cells / pathology; Tissue Engineering
TL;DR:
This review is designed for those in the tissue engineering and biomaterials fields who are interested in exploring the role MCs may play in wound-biomaterial interactions and wound healing and hopes to inspire more research in the MC-Biomaterial space in order to accelerate the design and construction of optimized implants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 8, 2021
2020 journal article
Mast Cell-Biomaterial Interactions and Tissue Repair
Tissue Engineering Part B: Reviews.
Source: ORCID
Added: November 11, 2020
2020 journal article
Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro
ACS Biomaterials Science & Engineering, 6(3), 1690–1703.
author keywords: extracellular matrix; hydrogels; vocal fold lamina propria; MBVs; fibrosis
MeSH headings : Animals; Biocompatible Materials / pharmacology; Fibroblasts; Mucous Membrane; Swine; Transforming Growth Factor beta1; Vocal Cords
TL;DR:
The results provide evidence of the unique protein composition of the VFLP-ECM and the potential link between the components of the vesicles and macromolecules and the inhibition of TGF-β1 signaling observed in vitro when transformed into injectable forms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: February 13, 2020
2019 journal article
Effects of polarized macrophages on the in vitro gene expression after Co-Culture of human pluripotent stem cell-derived cardiomyocytes
Journal of Immunology and Regenerative Medicine.
TL;DR:
In the present study, a cell-based system was used to study the in vitro interactions between lipopolysaccharide (LPS) and interferon-gamma (IFNγ)-activated Mϕs and human embryonic stem cell-derived cardiomyocytes (hESC-CMs).
(via Semantic Scholar)
Source: ORCID
Added: September 10, 2019
2018 journal article
Mesenchymal Stem Cell/Red Blood Cell-Inspired Nanoparticle Therapy in Mice with Carbon Tetrachloride-Induced Acute Liver Failure
ACS Nano, 12(7), 6536–6544.
Contributors: H. Liang n, K. Huang n , T. Su n, Z. Li n, S. Hu n, P. Dinh n, E. Wrona n, C. Shao *
author keywords: mesenchymal stem cells; red blood cells; biomimetic; nanoparticles; membrane cloaking; liver failure
MeSH headings : Animals; Apoptosis; Biomimetic Materials / chemistry; Biomimetic Materials / therapeutic use; Carbon Tetrachloride; Cell Line; Cell Proliferation; Disease Models, Animal; Erythrocyte Membrane / chemistry; Humans; Liver Failure, Acute / chemically induced; Liver Failure, Acute / pathology; Liver Failure, Acute / physiopathology; Liver Failure, Acute / therapy; Liver Regeneration; Male; Mesenchymal Stem Cells / chemistry; Mice; Mice, Inbred C57BL; Nanoparticles / chemistry; Nanoparticles / therapeutic use
TL;DR:
This work fabricated a nanoparticle that carries the beneficial regenerative factors from mesenchymal stem cells and further coated it with the membranes of red blood cells to increase blood stability and promote liver cell proliferation in vitro and have lower internalization by macrophage cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: September 10, 2019
2017 journal article
Macrophages' role in tissue disease and regeneration
Macrophages: Origin, Functions and Biointervention, 62, 245–271.
Source: NC State University Libraries
Added: August 6, 2018
2017 chapter
Macrophages’ role in tissue disease and regeneration
In Results and Problems in Cell Differentiation (Vol. 62, pp. 245–271).
Contributors: L. Gaffney n, P. Warren n, E. Wrona n, M. Fisher n & D. Freytes *
MeSH headings : Animals; Humans; Inflammation / immunology; Macrophages / immunology; Regeneration / immunology; Regenerative Medicine; Tissue Engineering; Wound Healing / immunology
TL;DR:
The role of macrophages in specific cases of injury and disease is described and enhanced therapeutic treatments can be developed that enhance the normal healing response as well as help the survival of therapeutic cells delivered to the site of injury.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: ORCID
Added: September 10, 2019
2017 review
Potential Synergistic Effects of Stem Cells and Extracellular Matrix Scaffolds
[Review of ]. ACS BIOMATERIALS SCIENCE & ENGINEERING, 4(4), 1208–1222.
Contributors: L. Gaffney n, E. Wrona n & D. Freytes n
author keywords: extracellular matrix (ECM); stem cells; pluripotent stem cells (PSCs); wound healing; macrophage polarization
TL;DR:
This work has shown that extracellular matrix-derived biomaterials, used as scaffolds to help regenerate diseased or damaged tissues, provide a scaffold material with native-like composition and structure through several mechanisms that include chemotactic properties, growth factor release, and modulation of the immune response.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2016 review
Extracellular Matrix for Vocal Fold Lamina Propria Replacement: A Review
[Review of ]. TISSUE ENGINEERING PART B-REVIEWS, 22(6), 421–429.
Contributors: E. Wrona n, R. Peng *, M. Amin *, R. Branski * & D. Freytes n
author keywords: voice; vocal fold; extracellular matrix; scaffold
MeSH headings : Extracellular Matrix; Humans; Mucous Membrane; Regenerative Medicine; Tissue Engineering; Vocal Cords
TL;DR:
This review explores the concept of tissue engineering as it relates to the VFs, as well as recent studies employing both naturally and synthetically derived biomaterials in combination with stem cells for a tissue-engineered approach to VF repair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
BMP protein-mediated crosstalk between inflammatory cells and human pluripotent stem cell-derived cardiomyocytes
Journal of Tissue Engineering and Regenerative Medicine, 11(5), 1466–1478.
Contributors: I. Pallotta *, B. Sun *, E. Wrona * & D. Freytes*
author keywords: cardiomyocytes; macrophages; inflammation; BMP; migration; tissue engineering; regenerative medicine
MeSH headings : Bone Morphogenetic Protein 4 / metabolism; Cell Communication; Humans; Inflammation / metabolism; Inflammation / pathology; Macrophages / metabolism; Macrophages / pathology; Myocytes, Cardiac / metabolism; Myocytes, Cardiac / pathology; Pluripotent Stem Cells / metabolism; Pluripotent Stem Cells / pathology; Smad Proteins / metabolism
TL;DR:
A new role is proposed for CM‐ and macrophage‐derived BMP proteins during the recruitment ofmacrophage subtypes and the maturation of repair cells, representing an important step towards creating a functional cardiac patch with superior therapeutic properties.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: ORCID, Crossref
Added: September 10, 2019
2015 journal article
Derivation and characterization of porcine vocal fold extracellular matrix scaffold
The Laryngoscope, 126(4), 928–935.
Contributors: E. Wrona *, R. Peng *, H. Born *, M. Amin *, R. Branski * & D. Freytes*
author keywords: Vocal fold; extracellular matrix; mesenchymal stem cells; scaffold
MeSH headings : Animals; Cells, Cultured; DNA / analysis; Deoxycholic Acid; Extracellular Matrix / physiology; Gene Expression; Mesenchymal Stem Cells / cytology; Peracetic Acid; Swine; Tissue Engineering / methods; Tissue Scaffolds; Vocal Cords / cytology
TL;DR:
To optimize decellularization of porcine vocal folds (VF) and quantify human bone marrow‐derived mesenchymal stem cell interactions with this matrix to provide a foundation for regenerative approaches to VF repair.
(via Semantic Scholar)
Sources: ORCID, Crossref
Added: September 10, 2019
2015 journal article
Differential gene expression in human, murine, and cell line-derived macrophages upon polarization
Experimental Cell Research, 347(1), 1–13.
Contributors: K. Spiller *, E. Wrona *, S. Romero-Torres, I. Pallotta *, P. Graney *, C. Witherel *, L. Panicker*, R. Feldman *
author keywords: Classically activated; Alternatively activated macrophages; Wound healing; In vitro characterization; Angiogenesis; Tissue engineering; Induced pluripotent stem cells; Biomaterials
MeSH headings : Animals; Biocompatible Materials / pharmacology; Cell Differentiation / drug effects; Cell Line; Cell Polarity / drug effects; Cell Polarity / genetics; Discriminant Analysis; Gene Expression Profiling; Gene Expression Regulation / drug effects; Humans; Interferon-gamma / pharmacology; Least-Squares Analysis; Lipopolysaccharides / pharmacology; Macrophages / cytology; Macrophages / drug effects; Macrophages / metabolism; Mice, Inbred BALB C; Phenotype
TL;DR:
Multivariate discriminant analysis was used to compare the in vitro expression of genes commonly chosen to assess macrophage phenotype across all three sources of macrophages, as well as those derived from induced pluripotent stem cells (iPSCs), that were polarized towards four distinct phenotypes using the same differentiation protocols.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities
(OpenAlex)
Sources: ORCID, Crossref
Added: September 10, 2019
2015 chapter
Role of mesenchymal stem cells, macrophages, and biomaterials during myocardial repair
In Biomaterials in Regenerative Medicine and the Immune System (pp. 1–15).
Contributors: I. Pallotta *, E. Wrona*, B. Sun * & D. Freytes *
TL;DR:
The inflammatory environment found after an MI is described followed by a description of how MSCs can be used as part of a cardiac patch that can modulate the inflammatory response to improve healing.
(via Semantic Scholar)
Source: ORCID
Added: September 10, 2019
2014 book
Natural cardiac extracellular matrix hydrogels for cultivation of human stem cell-derived cardiomyocytes
In Methods in Molecular Biology (Vol. 1181, pp. 69–81).
Contributors: D. Freytes *, . J.D. O'Neill, Y. Duan-Arnold*, E. Wrona* & G. Vunjak-Novakovic *
MeSH headings : Animals; Cell Count; Cell Culture Techniques / methods; Extracellular Matrix / chemistry; Flow Cytometry; Humans; Hydrogels / chemistry; Myocytes, Cardiac / cytology; Myocytes, Cardiac / metabolism; Staining and Labeling; Stem Cells / cytology; Tissue Engineering; Troponin T / metabolism
TL;DR:
It is described here how to obtain native ECM hydrogels from porcine hearts for the culture of human embryonic, induced pluripotent, and somatic stem cells for cardiac tissue engineering and regenerative medicine applications.
(via Semantic Scholar)
Source: ORCID
Added: September 10, 2019
Employment
Updated: July 5th, 2023 13:59
Booz Allen Hamilton VA, VA, US
Lead Scientist
Education
Updated: November 13th, 2019 14:26
North Carolina State University Raleigh, NC, US
PhD
Joint Department of Biomedical Engineering
University of Virginia Charlottesville, VA, US
BS
Biology
University of North Carolina at Chapel Hill Chapel Hill, NC, US
PhD
Joint Department of Biomedical Engineering
Funding History
Funding history based on the linked ORCID record. Updated: November 13th, 2019 14:29
award
Predoctoral Fellowship
American Heart Association
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