Eduardo Barbieri

College of Engineering

Works (17)

Updated: October 21st, 2024 08:34

2024 article

Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology

Sripada, S. A., Barbieri, E., Shastry, S., Wuestenhagen, E., Aldinger, A., Rammo, O., … Menegatti, S. (2024, May 28). ANALYTICAL CHEMISTRY, Vol. 5.

By: S. Sripada n, E. Barbieri n, S. Shastry n, E. Wuestenhagen, A. Aldinger*, O. Rammo*, M. Schulte*, M. Daniele n, S. Menegatti n 

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 11, 2024

2024 journal article

Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates

JOURNAL OF CHROMATOGRAPHY A, 1736.

By: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n, S. Menegatti n 

author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
Sources: Web Of Science, NC State University Libraries
Added: October 14, 2024

2024 article

Peptide ligands for the universal purification of exosomes by affinity chromatography

Kilgore, R. E., Moore, B. D., Sripada, S. A., Chu, W., Shastry, S., Barbieri, E., … Menegatti, S. (2024, August 4). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 8.

By: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , W. Tian * ...

author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 14, 2024

2024 journal article

Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

JOURNAL OF CHROMATOGRAPHY A, 1734.

By: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, G. Gilleskie n  ...

author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 1, 2024

2023 journal article

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 11(42), 15178–15191.

By: M. Sohail n, T. Pirzada n , R. Guenther n, E. Barbieri n, T. Sit n , S. Menegatti n , N. Crook n , C. Opperman n, S. Khan n 

author keywords: nonsporulating microbes; double yielding; Pseudomonas simiae; microstructure; controlledrelease
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 1, 2023

2023 journal article

Mechanically robust, thermally insulating and photo-responsive aerogels designed from sol-gel electrospun PVP-TiO2 nanofibers

APPLIED MATERIALS TODAY, 32.

By: V. Rahmanian n, T. Pirzada n , E. Barbieri n, S. Iftikhar n , F. Li n  & S. Khan n 

author keywords: Hybrid aerogel; Titania; Antibacterial; VOC decomposition; Thermal insulation; Oil-water separation
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 7, 2023

2023 article

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 7, 2023

2023 journal article

Peptide ligands targeting the vesicular stomatitis virus G (VSV-G) protein for the affinity purification of lentivirus particles

BIOTECHNOLOGY AND BIOENGINEERING, 121(2), 618–639.

By: E. Barbieri n, G. Mollica n, B. Moore n, S. Sripada n, S. Shastry n, R. Kilgore n, C. Loudermilk n, Z. Whitacre n ...

author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
TL;DR: This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024

2023 article

Peptonics: A new family of cell-protecting surfactants for the recombinant expression of therapeutic proteins in mammalian cell cultures

Zhang, K., Barbieri, E., Lebarre, J., Rameez, S., Mostafa, S., & Menegatti, S. (2023, October 18). BIOTECHNOLOGY JOURNAL, Vol. 10.

By: K. Zhang n, E. Barbieri n, J. Lebarre n, S. Rameez *, S. Mostafa* & S. Menegatti n 

author keywords: antibodies; mammalian cells; Peptonics; peptoids; Pluronics; surfactants
TL;DR: Peptonics, a novel family of peptide and peptoid surfactants whose monomer composition and sequence are designed to achieve high cell viability and productivity at a fraction of chain length and cost of Pluronics, can improve significantly purification of mAbs, thus increasing their availability at lower cost to patients. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023

2023 article

Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography

Shastry, S., Chu, W., Barbieri, E., Greback-Clarke, P., Smith, W. K., Cummings, C., … Menegatti, S. (2023, September 28). BIOTECHNOLOGY JOURNAL, Vol. 9.

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
TL;DR: This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 10, 2023

2023 journal article

The downstream bioprocess toolbox for therapeutic viral vectors

JOURNAL OF CHROMATOGRAPHY A, 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. Lebarre n, W. Chu n, J. O'Brien n, S. Menegatti n 

author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
TL;DR: This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

2023 article

Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy

2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.

By: J. Wang  n, M. Hosseini n, S. Shastry n, E. Barbieri n, W. Chu n, S. Menegatti n , M. Daniele n

author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
TL;DR: The preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 4, 2023

2023 article

Vapor phase synthesis of metal-organic frameworks on a nanofibrous aerogel creates enhanced functionality

Rahmanian, V., Ebrahim, M. Z. A., Razavi, S., Abdelmigeed, M., Barbieri, E., Menegatti, S., … Khan, S. A. (2023, November 6). JOURNAL OF MATERIALS CHEMISTRY A, Vol. 11.

By: V. Rahmanian n, M. Ebrahim  n, S. Razavi n, M. Abdelmigeed n, E. Barbieri n, S. Menegatti n , G. Parsons n , F. Li n , T. Pirzada n , S. Khan n 

Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 11, 2023

2022 journal article

Creating hierarchically porous banana paper-metal organic framework (MOF) composites with multifunctionality

APPLIED MATERIALS TODAY, 28.

By: S. Wang n, T. Pirzada n , W. Xie n, E. Barbieri n, O. Hossain n, C. Opperman n, L. Pal n , Q. Wei n , G. Parsons n , S. Khan n 

author keywords: lignocellulosic biomass; Banana fiber; Gas sorption; Antibacterial matrix; MOF-fiber composites; VOC sensor
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 22, 2022

2022 article

Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers

Barbieri, E., Cutright, C. C., Ramesh, S., Khan, S. A., Efimenko, K., Genzer, J., & Menegatti, S. (2022, August 23). ADVANCED MATERIALS INTERFACES, Vol. 8.

By: E. Barbieri n, C. Cutright n, S. Ramesh n, S. Khan n , K. Efimenko n , J. Genzer n , S. Menegatti n 

author keywords: antimicrobial coating; bacitracin; controlled release; E; coli; polymyxin B; S; aureus; surface functionalization
TL;DR: The proposed antibacterial fabric outperforms its commercial counterparts in terms of biocompatibility, showing virtually no adverse effect on human epidermal keratinocytes and demonstrate affordable and scalable routes for developing antimicrobial NWFs that efficiently eliminate resilient pathogenic bacteria. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 24, 2022

2022 journal article

Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

Membranes, 12(10), 944.

By: J. Fan n, E. Barbieri n, S. Shastry n, S. Menegatti n , C. Boi n  & R. Carbonell n

author keywords: adeno-associated virus (AAV); viral vector purification; nonwovens; membrane adsorber; anion exchange; cation exchange; membrane chromatography
TL;DR: A strong anion-exchange membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine, resulting in a quaternary amine ligand (AEX-TEA membrane) that exhibited a high AAV2 binding capacity and achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-ex exchange resin chromatography. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: November 14, 2022

2022 article

Study of Methods for the Synthesis of Pyrrole Derivatives and Evaluation of anti-Trypanosoma cruzi Activity

Barbieri, E., Sales Junior, P. A., Fonseca Murta, S. M., & Silva, B. V. (2022, March 22). REVISTA VIRTUAL DE QUIMICA.

By: E. Barbieri n, P. Sales Junior, S. Fonseca Murta * & B. Silva

author keywords: Pyrrole; Chagas disease; beta-ketoesters; chemical transformations
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: April 18, 2022

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