Eduardo Barbieri

College of Engineering

Works (24)

Updated: January 23rd, 2026 15:48

2026 article

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Idanwekhai, K. P., Shastry, S., Hurst, M. R., Minzoni, A., Barbieri, E., Remmler, L., … Tropsha, A. (2026, January 19). Biotechnology and Bioengineering, Vol. 1.

By: K. Idanwekhai*, S. Shastry n, M. Hurst n, A. Minzoni n, E. Barbieri*, L. Remmler*, E. Muratov, M. Daniele n, S. Menegatti n, A. Tropsha

Contributors: K. Idanwekhai*, S. Shastry n, M. Hurst n, A. Minzoni n, E. Barbieri*, L. Remmler*, E. Muratov, . al.

topics (OpenAlex): Protein purification and stability; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 21, 2026

2025 article

DendriPep Nanocoats: Substrate-Agnostic, Self-Assembling Constructs with Shear-Controlled Thickness and Permeability

Smith, R. J., Ramesh, S., Barbieri, E., O’Brien, J. N., Vallem, V., Dickey, M. D., … Menegatti, S. (2025, April 30). Langmuir, Vol. 4.

By: R. Smith n, S. Ramesh n, E. Barbieri n, J. O’Brien n, V. Vallem n, M. Dickey n, S. Khan n, J. Genzer n, C. Gorman n, S. Menegatti n

topics (OpenAlex): Dendrimers and Hyperbranched Polymers; Polymer Surface Interaction Studies; Silk-based biomaterials and applications
Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 30, 2025

2025 journal article

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine, 10(6).

By: E. Barbieri n, G. Mollica n, S. Sripada n, S. Shastry n, Y. Wu n, A. Minzoni n, W. Smith n, E. Wuestenhagen* ...

author keywords: affinity chromatography; lentivirus; membrane chromatography; peptide ligands; product polishing
topics (OpenAlex): Virus-based gene therapy research; CAR-T cell therapy research; Viral Infectious Diseases and Gene Expression in Insects
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 14, 2025

2025 journal article

Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification

Biotechnology and Bioengineering, 122(7), 1780–1792.

By: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n, S. Menegatti n

Contributors: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n, S. Menegatti n

author keywords: adenovirus; affinity chromatography; biomanufacturing; bioseparations; gene therapy
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Viral gastroenteritis research and epidemiology
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: May 6, 2025

2025 article

Integrating affinity chromatography in the platform process for Adenovirus purification

Wu, Y., Barbieri, E., Smith, W. K., Minzoni, A., Kilgore, R. E., Chu, W., … Menegatti, S. (2025, April 5). (Vol. 4). Vol. 4.

By: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n, S. Menegatti n

Contributors: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n, S. Menegatti n

topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability; Virus-based gene therapy research
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 18, 2026

2025 article

Modernizing the platform process of antibody purification for enhanced impurity clearance

LeBarre, J., Bhandari, D., Barbieri, E., Shu, Q., Li, X., Chandra, D., … Menegatti, S. (2025, November 20). Separation and Purification Technology, Vol. 11.

By: J. LeBarre n, D. Bhandari*, E. Barbieri*, Q. Shu n, X. Li*, D. Chandra*, X. Xu*, M. Phillips* ...

topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Viral Infectious Diseases and Gene Expression in Insects
Sources: NC State University Libraries, NC State University Libraries
Added: January 22, 2026

2025 article

Purification of CRISPR Cas12a from E. coli cell lysates using peptide affinity ligands

Wu, Y., Kilgore, R., Hetzler, Z., Chu, W., Moore, B., Barbieri, E., … Menegatti, S. (2025, May 17). Journal of Chromatography A, Vol. 1755.

By: Y. Wu n, R. Kilgore n, Z. Hetzler n, W. Chu n, B. Moore n, E. Barbieri n, Q. Wei n, S. Menegatti n

author keywords: CRISPR Cas12a; Gene editing; Peptide ligands; Affinity chromatography; Protein purification
MeSH headings : Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / enzymology; Chromatography, Affinity / methods; Ligands; CRISPR-Associated Proteins / isolation & purification; CRISPR-Associated Proteins / metabolism; CRISPR-Associated Proteins / chemistry; Peptides / chemistry; Peptides / metabolism; CRISPR-Cas Systems; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Bacterial Proteins / chemistry; Endodeoxyribonucleases / isolation & purification; Endodeoxyribonucleases / metabolism; Endodeoxyribonucleases / chemistry; Acidaminococcus / enzymology
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Advanced biosensing and bioanalysis techniques
Sources: Web Of Science, NC State University Libraries
Added: June 9, 2025

2024 journal article

Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology

Analytical Chemistry, 96(23), 9593–9600.

By: S. Sripada n, E. Barbieri n, S. Shastry n, E. Wuestenhagen*, A. Aldinger*, O. Rammo*, M. Schulte*, M. Daniele n, S. Menegatti n

Contributors: S. Sripada n, E. Barbieri n, S. Shastry n, E. Wuestenhagen*, A. Aldinger*, O. Rammo*, M. Schulte*, M. Daniele n, S. Menegatti n

MeSH headings : Lentivirus / genetics; Lentivirus / isolation & purification; Humans; Dynamic Light Scattering; Chromatography, Gel; HEK293 Cells; Particle Size; Genetic Vectors / genetics; Genetic Vectors / isolation & purification
topics (OpenAlex): Spectroscopy Techniques in Biomedical and Chemical Research; Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: June 11, 2024

2024 journal article

Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates

Journal of Chromatography A, 1736, 465396.

By: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n, S. Menegatti n

Contributors: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n, S. Menegatti n

author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
MeSH headings : Humans; Chromatography, Affinity / methods; Chlorocebus aethiops; Animals; Ligands; Vero Cells; HEK293 Cells; Peptides / chemistry; Peptides / isolation & purification; Peptides / metabolism; Adenoviridae / chemistry; Adenoviridae / isolation & purification; Capsid Proteins / isolation & purification; Capsid Proteins / metabolism; Capsid Proteins / chemistry
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 14, 2024

2024 journal article

Peptide ligands for the universal purification of exosomes by affinity chromatography

Biotechnology and Bioengineering, 121(11), 3484–3501.

By: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n, W. Tian* ...

Contributors: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n, . al.

author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
MeSH headings : Exosomes / chemistry; Exosomes / metabolism; Chromatography, Affinity / methods; Ligands; Humans; Peptides / chemistry; Peptides / metabolism; Peptides / isolation & purification
topics (OpenAlex): Extracellular vesicles in disease; RNA Interference and Gene Delivery; Inhalation and Respiratory Drug Delivery; Lipid Membrane Structure and Behavior
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 14, 2024

2024 journal article

Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

Journal of Chromatography A, 1734, 465320.

By: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, G. Gilleskie n ...

Contributors: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, . al.

author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
MeSH headings : Dependovirus / isolation & purification; Dependovirus / genetics; Dependovirus / chemistry; Chromatography, Affinity / methods; Peptides / chemistry; Peptides / isolation & purification; Serogroup; Humans; Hydrogen-Ion Concentration; Genetic Vectors; HEK293 Cells
topics (OpenAlex): Virus-based gene therapy research; RNA Interference and Gene Delivery; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: September 1, 2024

2023 journal article

Cellulose Acetate-Stabilized Pickering Emulsions: Preparation, Rheology, and Incorporation of Agricultural Active Ingredients

ACS Sustainable Chemistry & Engineering, 11(42), 15178–15191.

By: M. Sohail n, T. Pirzada n, R. Guenther n, E. Barbieri n, T. Sit n, S. Menegatti n, N. Crook n, C. Opperman n, S. Khan n

author keywords: nonsporulating microbes; double yielding; Pseudomonas simiae; microstructure; controlledrelease
topics (OpenAlex): Pickering emulsions and particle stabilization; Advanced Cellulose Research Studies; Proteins in Food Systems
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: October 1, 2023

2023 journal article

Mechanically robust, thermally insulating and photo-responsive aerogels designed from sol-gel electrospun PVP-TiO2 nanofibers

Applied Materials Today, 32, 101784.

By: V. Rahmanian n, T. Pirzada n, E. Barbieri n, S. Iftikhar n, F. Li n & S. Khan n

Contributors: V. Rahmanian n, T. Pirzada n, E. Barbieri n, S. Iftikhar n, F. Li n & S. Khan n

author keywords: Hybrid aerogel; Titania; Antibacterial; VOC decomposition; Thermal insulation; Oil-water separation
topics (OpenAlex): Aerogels and thermal insulation; Advanced Sensor and Energy Harvesting Materials; TiO2 Photocatalysis and Solar Cells
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: March 7, 2023

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 journal article

Peptide ligands targeting the vesicular stomatitis virus G (VSV‐G) protein for the affinity purification of lentivirus particles

Biotechnology and Bioengineering, 121(2), 618–639.

By: E. Barbieri n, G. Mollica n, B. Moore n, S. Sripada n, S. Shastry n, R. Kilgore n, C. Loudermilk n, Z. Whitacre n ...

author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
MeSH headings : Animals; Humans; Lentivirus / genetics; Lentivirus / metabolism; Vesicular Stomatitis; HEK293 Cells; Peptides / metabolism; Vesiculovirus / genetics; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Virology and Viral Diseases; Bacteriophages and microbial interactions
TL;DR: This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 29, 2024

2023 article

Peptonics: A new family of cell‐protecting surfactants for the recombinant expression of therapeutic proteins in mammalian cell cultures

Zhang, K., Barbieri, E., LeBarre, J., Rameez, S., Mostafa, S., & Menegatti, S. (2023, October 16). Biotechnology Journal, Vol. 10.

By: K. Zhang n, E. Barbieri n, J. LeBarre n, S. Rameez*, S. Mostafa* & S. Menegatti n

author keywords: antibodies; mammalian cells; Peptonics; peptoids; Pluronics; surfactants
MeSH headings : Cricetinae; Animals; Humans; Cricetulus; Poloxamer; Surface-Active Agents / pharmacology; CHO Cells; Antibodies, Monoclonal / chemistry; Cell Culture Techniques; Recombinant Proteins / genetics; Recombinant Proteins / pharmacology
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability; Monoclonal and Polyclonal Antibodies Research
TL;DR: Peptonics, a novel family of peptide and peptoid surfactants whose monomer composition and sequence are designed to achieve high cell viability and productivity at a fraction of chain length and cost of Pluronics, can improve significantly purification of mAbs, thus increasing their availability at lower cost to patients. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023

2023 journal article

Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography

Biotechnology Journal, 19(1).

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback‐Clarke n, W. Smith n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

Contributors: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith n, C. Cummings n, A. Minzoni n, . al.

author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
MeSH headings : Humans; Dependovirus / genetics; Capsid / chemistry; HEK293 Cells; Transduction, Genetic; Peptides / metabolism; Ligands; Chromatography, Affinity; Genetic Vectors / genetics
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
TL;DR: This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 10, 2023

2023 article

The downstream bioprocess toolbox for therapeutic viral vectors

Kilgore, R., Minzoni, A., Shastry, S., Smith, W., Barbieri, E., Wu, Y., … Menegatti, S. (2023, September 9). Journal of Chromatography A, Vol. 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. LeBarre n, W. Chu n, J. O'Brien n, S. Menegatti n

author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Bacteriophages and microbial interactions
TL;DR: This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

2023 article

Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy

Wang, J., Hosseini, M., Shastry, S., Barbieri, E., Chu, W., Menegatti, S., & Daniele, M. A. (2023, July 30). 2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS.

By: J. Wang n, M. Hosseini n, S. Shastry n, E. Barbieri n, W. Chu n, S. Menegatti n, M. Daniele n

Contributors: J. Wang n, M. Hosseini n, S. Shastry n, E. Barbieri n, W. Chu n, S. Menegatti n, M. Daniele n

author keywords: adeno-associated virus; AAV2; immunosensor; electrochemical impedance spectroscopy; viral vector
topics (OpenAlex): Virus-based gene therapy research; Viral Infections and Immunology Research; RNA Interference and Gene Delivery
TL;DR: The preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 4, 2023

2023 journal article

Vapor phase synthesis of metal–organic frameworks on a nanofibrous aerogel creates enhanced functionality

Journal of Materials Chemistry A, 12(1), 214–226.

By: V. Rahmanian n, M. Ahmad Ebrahim n, S. Razavi n, M. Abdelmigeed n, E. Barbieri n, S. Menegatti n, G. Parsons n, F. Li n, T. Pirzada n, S. Khan n

topics (OpenAlex): Metal-Organic Frameworks: Synthesis and Applications; Covalent Organic Framework Applications; Membrane Separation and Gas Transport
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: November 11, 2023

2022 article

Creating hierarchically porous banana paper-metal organic framework (MOF) composites with multifunctionality

Wang, S., Pirzada, T., Xie, W., Barbieri, E., Hossain, O., Opperman, C. H., … Khan, S. A. (2022, May 21). Applied Materials Today, Vol. 28, p. 101517.

By: S. Wang n, T. Pirzada n, W. Xie n, E. Barbieri n, O. Hossain n, C. Opperman n, L. Pal n, Q. Wei n, G. Parsons n, S. Khan n

author keywords: lignocellulosic biomass; Banana fiber; Gas sorption; Antibacterial matrix; MOF-fiber composites; VOC sensor
topics (OpenAlex): Metal-Organic Frameworks: Synthesis and Applications; Gas Sensing Nanomaterials and Sensors; Covalent Organic Framework Applications
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 22, 2022

2022 article

Potent Antibacterial Composite Nonwovens Functionalized with Bioactive Peptides and Polymers

Barbieri, E., Cutright, C. C., Ramesh, S., Khan, S. A., Efimenko, K., Genzer, J., & Menegatti, S. (2022, August 23). Advanced Materials Interfaces, Vol. 8.

By: E. Barbieri n, C. Cutright n, S. Ramesh n, S. Khan n, K. Efimenko n, J. Genzer n, S. Menegatti n

author keywords: antimicrobial coating; bacitracin; controlled release; E; coli; polymyxin B; S; aureus; surface functionalization
topics (OpenAlex): Antimicrobial Peptides and Activities; Antimicrobial agents and applications; Polydiacetylene-based materials and applications
TL;DR: The proposed antibacterial fabric outperforms its commercial counterparts in terms of biocompatibility, showing virtually no adverse effect on human epidermal keratinocytes and demonstrate affordable and scalable routes for developing antimicrobial NWFs that efficiently eliminate resilient pathogenic bacteria. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 24, 2022

2022 journal article

Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

Membranes, 12(10), 944.

By: J. Fan n, E. Barbieri n, S. Shastry n, S. Menegatti n, C. Boi n & R. Carbonell n

author keywords: adeno-associated virus (AAV); viral vector purification; nonwovens; membrane adsorber; anion exchange; cation exchange; membrane chromatography
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Virus-based gene therapy research; Viral gastroenteritis research and epidemiology
TL;DR: A strong anion-exchange membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine, resulting in a quaternary amine ligand (AEX-TEA membrane) that exhibited a high AAV2 binding capacity and achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-ex exchange resin chromatography. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: November 14, 2022

2022 article

Study of Methods for the Synthesis of Pyrrole Derivatives and Evaluation of anti-Trypanosoma cruzi Activity

Barbieri, E., Junior, P. A. S., Murta, S. M. F., & Silva, B. V. S. V. (2022, January 1). Revista Virtual De Química.

By: E. Barbieri n, P. Junior*, S. Murta* & B. Silva

author keywords: Pyrrole; Chagas disease; beta-ketoesters; chemical transformations
topics (OpenAlex): Synthesis and Characterization of Pyrroles
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: April 18, 2022

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