Eli Dwayne Buckner

Works (4)

Updated: April 5th, 2024 18:54

2021 review

High-throughput image segmentation and machine learning approaches in the plant sciences across multiple scales

(J. M. Jez & C. N. Topp, Eds.) [Review of ]. EMERGING TOPICS IN LIFE SCIENCES, 5(2), 239–248.

By: E. Buckner n, H. Tong n, C. Ottley n  & C. Williams n 

Ed(s): J. Jez & C. Topp

MeSH headings : Machine Learning; Phenotype; Plant Development; Plants / genetics; Stress, Physiological
TL;DR: It is shown how the methodologies for segmentation and classification differ due to the diversity of physical characteristics found at these different scales, and how the biological mechanisms by which plants respond to abiotic/biotic stresses or genotypic modifications can be extracted from these approaches. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 21, 2021

2020 chapter

BioVision Tracker: A semi-automated image analysis software for spatiotemporal gene expression tracking in Arabidopsis thaliana

In Methods in Cell Biology (Vol. 160, pp. 419–436).

By: E. Buckner n, I. Madison n, C. Melvin n, T. Long n , R. Sozzani  n & C. Williams n 

Contributors: E. Buckner n, I. Madison n, C. Melvin n, T. Long n , R. Sozzani n  & C. Williams n 

MeSH headings : Arabidopsis / genetics; Automation; Gene Expression Regulation, Plant; Image Processing, Computer-Assisted; Plant Roots / anatomy & histology; Software; Time Factors
TL;DR: A procedure for analyzing 3D microscopy images to track spatiotemporal gene expression signals using the MATLAB-based BioVision Tracker software, which requires either a fluorescence image or a brightfield image to analyze root geometry and afluorescence image to capture and track temporal changes in gene expression. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: June 17, 2020

2020 chapter

MAGIC: Live imaging of cellular division in plant seedlings using lightsheet microscopy

In Methods in Cell Biology (Vol. 160, pp. 405–418).

By: I. Madison n, C. Melvin n, E. Buckner n, C. Williams n , R. Sozzani  n & T. Long n 

Contributors: I. Madison n, C. Melvin n, E. Buckner n, C. Williams n , R. Sozzani n  & T. Long n 

MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; Cell Division; Imaging, Three-Dimensional; Microscopy, Fluorescence / methods; Seedlings / cytology; Seedlings / growth & development
TL;DR: This chapter describes a detailed method for preparing and imaging Arabidopsis thaliana seedlings for lightsheet microscopy via a Multi-Sample Imaging Growth Chamber (MAGIC), which allows simultaneous imaging of at least four samples. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: June 17, 2020

2019 journal article

Stem-cell-ubiquitous genes spatiotemporally coordinate division through regulation of stem-cell-specific gene networks

NATURE COMMUNICATIONS, 10.

By: N. Clark n, E. Buckner n, A. Fisher n, E. Nelson n, T. Nguyen n, A. Simmons *, M. Balaguer n, T. Butler-Smith n ...

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Asymmetric Cell Division / genetics; Asymmetric Cell Division / physiology; Cell Differentiation; Cell Division; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks / genetics; Plant Roots / cytology; Plant Roots / genetics; Plant Roots / growth & development; Stem Cells / cytology; Stem Cells / metabolism; Transcription Factors / metabolism; Transcriptome; Ubiquitination / genetics; Ubiquitins / genetics
TL;DR: In the Arabidopsis root, a stem-cell-ubiquitous gene, TESMIN-LIKE CXC2 (TCX2), controls stem cell division by regulating stem cell-type specific networks and orchestrates the coordinated division of different stem cell types. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 30, 2019

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