@article{durmaz_hu_aroian_klaenhammer_2016, title={Intracellular and Extracellular Expression of Bacillus thuringiensis Crystal Protein Cry5B in Lactococcus lactis for Use as an Anthelminthic}, volume={82}, ISSN={["1098-5336"]}, DOI={10.1128/aem.02365-15}, abstractNote={ABSTRACT}, number={4}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Durmaz, Evelyn and Hu, Yan and Aroian, Raffi V. and Klaenhammer, Todd R.}, year={2016}, month={Feb}, pages={1286–1294} } @article{baugher_durmaz_klaenhammer_2014, title={Spontaneously Induced Prophages in Lactobacillus gasseri Contribute to Horizontal Gene Transfer}, volume={80}, ISSN={["1098-5336"]}, DOI={10.1128/aem.04092-13}, abstractNote={ABSTRACT}, number={11}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Baugher, J. L. and Durmaz, E. and Klaenhammer, T. R.}, year={2014}, month={Jun}, pages={3508–3517} } @article{mohamadzadeh_durmaz_zadeh_pakanati_gramarossa_cohran_klaenhammer_2010, title={Targeted expression of anthrax protective antigen by Lactobacillus gasseri as an anthrax vaccine}, volume={5}, ISSN={["1746-0921"]}, DOI={10.2217/fmb.10.78}, abstractNote={ Aim: Induction of protective immunity against pathogenic microbes, including Bacillus anthracis, requires efficient vaccines that potentiate antibody avidity and increase T-cell longevity. We recently reported that the delivery of targeted B. anthracis protective antigen (PA) genetically fused to a DC-binding peptide (DCpep) by Lactobacillus acidophilus induced mucosal and systemic immunity against B. anthracis challenge in mice. Materials & methods: Improvement of this oral vaccine strategy was attempted by use of the high copy and genetically stable q-replicating vector, pTRKH2, for expression of the targeted PA fusion protein in Lactobacillus gasseri, a common human commensal microbe, to vaccinate animals against anthrax Sterne infection. Results: Oral application of L. gasseri expressing the PA–DCpep fusion proteins elicited robust PA-neutralizing antibody and T-cell mediated immune responses against anthrax Sterne challenge, resulting in complete animal survival. Collectively, this improved expression vaccine strategy reduced the number of inoculations and length of the boosting period, leading to animal protection via efficacious bacterial adjuvanticity and safe oral delivery of this vaccine to mucosal immune cells, including dendritic cells. Conclusion: Lactobacillus-based delivery offers tremendous practical advantages. Recombinant antigens such as PA would not require chemical coupling agents, and the recombinant bacteria can be administered orally where upon both mucosal and systemic immune responses are elicited. }, number={8}, journal={FUTURE MICROBIOLOGY}, author={Mohamadzadeh, Mansour and Durmaz, Evelyn and Zadeh, Mojgan and Pakanati, Krishna Chaitanya and Gramarossa, Matthew and Cohran, Valeria and Klaenhammer, Todd R.}, year={2010}, month={Aug}, pages={1289–1296} } @article{goh_azcarate-peril_o'flaherty_durmaz_valence_jardin_lortal_klaenhammer_2009, title={Development and Application of a upp-Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM}, volume={75}, ISSN={["1098-5336"]}, DOI={10.1128/AEM.02502-08}, abstractNote={ABSTRACT}, number={10}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Goh, Yong Jun and Azcarate-Peril, M. Andrea and O'Flaherty, Sarah and Durmaz, Evelyn and Valence, Florence and Jardin, Julien and Lortal, Sylvie and Klaenhammer, Todd R.}, year={2009}, month={May}, pages={3093–3105} } @article{durmaz_miller_azcarate-peril_toon_klaenhammer_2008, title={Genome sequence and characteristics of Lrm1, a prophage from industrial Lactobacillus rhamnosus strain M1}, volume={74}, ISSN={["1098-5336"]}, DOI={10.1128/AEM.00010-08}, abstractNote={ABSTRACT}, number={15}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Durmaz, Evelyn and Miller, Michael J. and Azcarate-Peril, M. Andrea and Toon, Stephen P. and Klaenhammer, Todd R.}, year={2008}, month={Aug}, pages={4601–4609} } @misc{durmaz_klaenhammer_2007, title={AbiZ phage resistance gene}, volume={7,169,911}, number={2007 Jan. 30}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Durmaz, E. and Klaenhammer, T. R.}, year={2007} } @article{durmaz_klaenhammer_2007, title={Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature lysis of phage-infected Lactococcus lactis}, volume={189}, ISSN={["1098-5530"]}, DOI={10.1128/JB.00904-06}, abstractNote={ABSTRACT}, number={4}, journal={JOURNAL OF BACTERIOLOGY}, author={Durmaz, Evelyn and Klaenhammer, Todd R.}, year={2007}, month={Feb}, pages={1417–1425} } @article{durmaz_madsen_israelsen_klaenhammer_2002, title={Lactococcus lactis lytic bacteriophages of the p335 group are inhibited by overexpression of a truncated CI repressor}, volume={184}, ISSN={["0021-9193"]}, DOI={10.1128/JB.184.23.6532-6543.2002}, abstractNote={ABSTRACT}, number={23}, journal={JOURNAL OF BACTERIOLOGY}, author={Durmaz, E and Madsen, SA and Israelsen, H and Klaenhammer, TR}, year={2002}, month={Dec}, pages={6532–6543} } @article{durmaz_klaenhammer_2000, title={Genetic analysis of chromosomal regions of Lactococcus lactis acquired by recombinant lytic phages}, volume={66}, ISSN={["0099-2240"]}, DOI={10.1128/AEM.66.3.895-903.2000}, abstractNote={ABSTRACT}, number={3}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Durmaz, E and Klaenhammer, TR}, year={2000}, month={Mar}, pages={895–903} } @article{durmaz_klaenhammer_1995, title={A starter culture rotation strategy incorporating paired restriction/modification and abortive infection bacteriophage defenses in a single Lactococcus lactis strain}, volume={61}, number={4}, journal={Applied and Environmental Microbiology}, author={Durmaz, E. and Klaenhammer, T. R.}, year={1995}, pages={1266} } @article{durmaz_higgins_klaenhammer_1992, title={MOLECULAR CHARACTERIZATION OF A 2ND ABORTIVE PHAGE RESISTANCE GENE PRESENT IN LACTOCOCCUS-LACTIS SUBSP LACTIS ME2}, volume={174}, ISSN={["0021-9193"]}, DOI={10.1128/jb.174.22.7463-7469.1992}, abstractNote={The fifth phage resistance factor from the prototype phage-insensitive strain Lactococcus lactis subsp. lactis ME2 has been characterized and sequenced. The genetic determinant for Prf (phage resistance five) was subcloned from the conjugative plasmid pTN20, which also encodes a restriction and modification system. Typical of other abortive resistance mechanisms, Prf reduces the efficiency of plaquing to 10(-2) to 10(-3) and decreases the plaque size and burst size of the small isometric-headed phage p2 in L. lactis subsp. lactis LM0230. However, normal-size plaques occurred at a frequency of 10(-4) and contained mutant phages that were resistant to Prf, even after repeated propagation through a sensitive host. Prf does not prevent phage adsorption or promote restriction and modification activities, but 90% of Prf+ cells infected with phage p2 die. Thus, phage infections in Prf+ cells are aborted. Prf is effective in both L. lactis subsp. lactis and L. lactis subsp. cremoris strains against several small isometric-headed phages but not against prolate-headed phages. The Prf determinant was localized by Tn5 mutagenesis and subcloning. DNA sequencing identified a 1,056-nucleotide structural gene designated abiC. Prf+ expression was obtained when abiC was subcloned into the lactococcal expression vector pMG36e. abiC is distinct from two other lactococcal abortive phage resistance genes, abiA (Hsp+, from L. lactis subsp. lactis ME2) and abi416 (Abi+, from L. lactis subsp. lactis IL416). Unlike abiA, the action of abiC does not appear to affect DNA replication. Thus, abiC represents a second abortive system found in ME2 that acts at a different point of the phage lytic cycle.}, number={22}, journal={JOURNAL OF BACTERIOLOGY}, author={DURMAZ, E and HIGGINS, DL and KLAENHAMMER, TR}, year={1992}, month={Nov}, pages={7463–7469} } @article{durmaz_klaenhammer_1991, title={A fourth mechanism for bacteriophage resistance in Lactococcus lactis subsp. lactis ME2}, volume={74}, journal={Journal of Dairy Science}, author={Durmaz, E. and Klaenhammer, T. R.}, year={1991}, pages={120} }