@misc{laosuntisuk_elorriaga_doherty_2023, title={The Game of Timing: Circadian Rhythms Intersect with Changing Environments}, volume={74}, ISSN={["1545-2123"]}, DOI={10.1146/annurev-arplant-070522-065329}, abstractNote={Recurring patterns are an integral part of life on Earth. Through evolution or breeding, plants have acquired systems that coordinate with the cyclic patterns driven by Earth's movement through space. The biosystem responses to these physical rhythms result in biological cycles of daily and seasonal activity that feed back into the physical cycles. Signaling networks to coordinate growth and molecular activities with these persistent cycles have been integrated into plant biochemistry. The plant circadian clock is the coordinator of this complex, multiscale, temporal schedule. However, we have detailed knowledge of the circadian clock components and functions in only a few species under controlled conditions. We are just beginning to understand how the clock functions in real-world conditions. This review examines what we know about the circadian clock in diverse plant species, the challenges with extrapolating data from controlled environments, and the need to anticipate how plants will respond to climate change.}, journal={ANNUAL REVIEW OF PLANT BIOLOGY}, author={Laosuntisuk, Kanjana and Elorriaga, Estefania and Doherty, Colleen J.}, year={2023}, pages={511–538} }
 @article{elorriaga_klocko_ma_du plessis_an_myburg_strauss_2021, title={Genetic containment in vegetatively propagated forest trees: CRISPR disruption of LEAFY function in Eucalyptus gives sterile indeterminate inflorescences and normal juvenile development}, volume={19}, ISSN={["1467-7652"]}, DOI={10.1111/pbi.13588}, abstractNote={Summary Eucalyptus is among the most widely planted taxa of forest trees worldwide. However, its spread as an exotic or genetically engineered form can create ecological and social problems. To mitigate gene flow via pollen and seeds, we mutated the Eucalyptus orthologue of LEAFY (LFY) by transforming a Eucalyptus grandis × urophylla wild‐type hybrid and two Flowering Locus T (FT) overexpressing (and flowering) lines with CRISPR Cas9 targeting its LFY orthologue, ELFY . We achieved high rates of elfy biallelic knockouts, often approaching 100% of transgene insertion events. Frameshift mutations and deletions removing conserved amino acids caused strong floral alterations, including indeterminacy in floral development and an absence of male and female gametes. These mutants were otherwise visibly normal and did not differ statistically from transgenic controls in juvenile vegetative growth rate or leaf morphology in greenhouse trials. Genes upstream or near to ELFY in the floral development pathway were overexpressed, whereas floral organ identity genes downstream of ELFY were severely depressed. We conclude that disruption of ELFY function appears to be a useful tool for sexual containment, without causing statistically significant or large adverse effects on juvenile vegetative growth or leaf morphology.}, number={9}, journal={PLANT BIOTECHNOLOGY JOURNAL}, author={Elorriaga, Estefania and Klocko, Amy L. and Ma, Cathleen and Du Plessis, Marc and An, Xinmin and Myburg, Alexander A. and Strauss, Steven H.}, year={2021}, month={Sep}, pages={1743–1755} }