@article{egorov_griffin_styles_kobylanski_klein_wickersham_ritter_sams_hudgens_wade_2024, title={Time outdoors and residential greenness are associated with reduced systemic inflammation and allostatic load}, volume={344}, ISSN={["1873-6424"]}, url={https://doi.org/10.1016/j.envpol.2024.123408}, DOI={10.1016/j.envpol.2024.123408}, abstractNote={Contacts with nature are linked with reduced morbidity and mortality. Hypothesized pathways include relaxation, physical activity, and improved immune function. This cross-sectional study of 320 adults in central North Carolina assessed health benefits of residential greenness using allostatic load (AL) and systemic inflammation (INFL) indices, composite biomarker-based measures of physiological dysregulation and inflammation, respectively. Distance-to-residence weighted tree cover and vegetated land cover measures were estimated within 500 m of each residence; 37 biomarkers of immune, neuroendocrine, cardiovascular, and metabolic functions were dichotomized at distribution or health-based cut-offs. AL was calculated as a sum of potentially unhealthy values of all biomarkers; INFL was based on a sub-set of 18 immune biomarkers. Regression analysis used generalized additive models for Poisson-distributed outcome. An interquartile range (IQR) increase in tree cover was associated with 0.89 (95 % Confidence Limits 0.82; 0.97) and 0.90 (0.79; 1.03)-fold change in AL and INFL, respectively. Greater daily outdoor time was associated with reduced AL and INFL, while leisure screen time, problems with sleeping, and common chronic infections were linked with increased AL and INFL. Among 138 individuals spending more than 1 h outdoors daily, an IQR increase in tree cover was associated with 0.76 (0.67; 0.86) and 0.81 (0.65; 1.02)-fold changes in AL and INFL, respectively. Among individuals with residential tree cover above the 50th percentile, spending more than 3 h outdoors daily was associated with 0.54 (0.37; 0.78) and 0.28 (0.15; 0.54)-fold changes in AL and INFL, respectively, compared to spending less than 30 min outdoors; there were no significant effects in the low tree cover stratum. Consistent but weaker effects were observed for vegetated land cover. Interaction effects of tree and vegetative cover and time spent outdoors on AL and INFL were statistically significant. This biomarker-based approach can help to assess public health benefits of green spaces.}, journal={ENVIRONMENTAL POLLUTION}, author={Egorov, Andrey I. and Griffin, Shannon M. and Styles, Jennifer N. and Kobylanski, Jason and Klein, Jo and Wickersham, Lindsay and Ritter, Rebecca and Sams, Elizabeth and Hudgens, Edward E. and Wade, Timothy J.}, year={2024}, month={Mar} }
 @article{styles_egorov_grif_klein_scott_sams_hudgens_mugford_stewart_lu_et al._2023, title={Greener residential environment is associated with increased bacterial diversity in outdoor ambient air}, volume={880}, ISSN={["1879-1026"]}, DOI={10.1016/j.scitotenv.2023.163266}, abstractNote={In urban areas, exposure to greenspace has been found to be beneficial to human health. The biodiversity hypothesis proposed that exposure to diverse ambient microbes in greener areas may be one pathway leading to health benefits such as improved immune system functioning, reduced systemic inflammation, and ultimately reduced morbidity and mortality. Previous studies observed differences in ambient outdoor bacterial diversity between areas of high and low vegetated land cover but didn't focus on residential environments which are important to human health. This research examined the relationship between vegetated land and tree cover near residence and outdoor ambient air bacterial diversity and composition. We used a filter and pump system to collect ambient bacteria samples outside residences in the Raleigh-Durham-Chapel Hill metropolitan area and identified bacteria by 16S rRNA amplicon sequencing. Geospatial quantification of total vegetated land or tree cover was conducted within 500 m of each residence. Shannon's diversity index and weighted UniFrac distances were calculated to measure α (within-sample) and β (between-sample) diversity, respectively. Linear regression for α-diversity and permutational analysis of variance (PERMANOVA) for β-diversity were used to model relationships between vegetated land and tree cover and bacterial diversity. Data analysis included 73 ambient air samples collected near 69 residences. Analysis of β-diversity demonstrated differences in ambient air microbiome composition between areas of high and low vegetated land (p = 0.03) and tree cover (p = 0.07). These relationships remained consistent among quintiles of vegetated land (p = 0.03) and tree cover (p = 0.008) and continuous measures of vegetated land (p = 0.03) and tree cover (p = 0.03). Increased vegetated land and tree cover were also associated with increased ambient microbiome α-diversity (p = 0.06 and p = 0.03, respectively). To our knowledge, this is the first study to demonstrate associations between vegetated land and tree cover and the ambient air microbiome's diversity and composition in the residential ecosystem.}, journal={SCIENCE OF THE TOTAL ENVIRONMENT}, author={Styles, Jennifer N. and Egorov, Andrey I. and Grif, Shannon M. and Klein, Jo and Scott, J. W. and Sams, Elizabeth A. and Hudgens, Edward and Mugford, Chris and Stewart, Jill R. and Lu, Kun and et al.}, year={2023}, month={Jul} }
 @misc{klein_2021, title={2020 Census data update}, url={https://uncgreference.blogspot.com/2021/08/2020-census-data-update.html}, journal={The Ref Desk}, author={Klein, J.}, year={2021}, month={Aug} }
 @inproceedings{klein_carlton_2021, title={Capturing “science everywhere”: Get into nature with iNaturalist}, author={Klein, J. and Carlton, M.}, year={2021}, month={Apr} }
 @inproceedings{klein_carlton_2021, title={Crawfishing for data: Using iNaturalist for data literacy instruction}, url={http://libres.uncg.edu/ir/uncg/listing.aspx?id=36719}, author={Klein, J. and Carlton, M.}, year={2021}, month={Oct} }
 @inproceedings{klein_2021, title={Data management}, author={Klein, J.}, year={2021}, month={Mar} }
 @inproceedings{exner_klein_gypin_2021, title={Data management planning with DMPTool}, author={Exner, N. and Klein, J. and Gypin, L.}, year={2021}, month={Oct} }
 @misc{klein_2021, title={Data visualization & design}, author={Klein, J.}, year={2021}, month={Feb} }
 @inproceedings{carlton_yisak_klein_2021, title={Engaging with STEM during COVID}, author={Carlton, M. and Yisak, R. and Klein, J.}, year={2021}, month={May} }
 @inbook{carlton_klein_2021, place={Chicago, IL}, title={Keeping track of your recipe: Moving from cookbooks to the web}, ISBN={9780838938478}, booktitle={The Scholarly Communications Cookbook}, publisher={Association of College and Research Libraries}, author={Carlton, M. and Klein, J.}, editor={Buljung, B. and Bongiovanni, E.Editors}, year={2021}, pages={293–295} }
 @inproceedings{klein_carlton_2021, title={Learning data "naturally": Engaging communities in the scientific process with iNaturalist}, author={Klein, J. and Carlton, M.}, year={2021}, month={Aug} }
 @inproceedings{klein_2021, title={Lessons learned: Data viz and communicating research}, author={Klein, J.}, year={2021}, month={Jan} }
 @inproceedings{klein_rood_2021, title={Pass the button maker: Supporting LGBTQIA+ patrons and library employees with low stakes, high impact programming}, author={Klein, J. and Rood, M.}, year={2021}, month={Apr} }
 @article{egorov_converse_griffin_bonasso_wickersham_klein_kobylanski_ritter_styles_ward_et al._2021, title={Recreational water exposure and waterborne infections in a prospective salivary antibody study at a Lake Michigan beach}, volume={11}, url={http://dx.doi.org/10.1038/s41598-021-00059-2}, DOI={10.1038/s41598-021-00059-2}, abstractNote={Abstract}, number={1}, journal={Scientific Reports}, publisher={Springer Science and Business Media LLC}, author={Egorov, Andrey I. and Converse, Reagan and Griffin, Shannon M. and Bonasso, Russell and Wickersham, Lindsay and Klein, Elizabeth and Kobylanski, Jason and Ritter, Rebecca and Styles, Jennifer N. and Ward, Honorine and et al.}, year={2021}, month={Dec} }
 @misc{klein_2021, title={WorldCat Discovery error when searching}, url={https://uncgreference.blogspot.com/2021/08/worldcat-discovery-error-when-searching.html}, journal={The Ref Desk}, author={Klein, J.}, year={2021}, month={Aug} }
 @inproceedings{klein_2020, title={"Is anyone there?”: Using Discord to facilitate networking and discussion at virtual conferences}, author={Klein, J.}, year={2020}, month={Jul} }
 @inproceedings{grynoch_klein_rod_2020, title={Connecting communities through a passion for data visualization in libraries: The Visualizing the Future Symposia}, author={Grynoch, T. and Klein, J. and Rod, A.}, year={2020}, month={Mar} }
 @inproceedings{klein_2020, title={EnviroAtlas: A powerful tool for environmental justice and planning}, author={Klein, J.}, year={2020}, month={Feb} }
 @inproceedings{klein_2020, title={Ethics and empathy in data visualization}, author={Klein, J.}, year={2020}, month={May} }
 @article{greater tree cover near residence is associated with reduced allostatic load in residents of central north carolina_2020, url={http://dx.doi.org/10.1016/j.envres.2020.109435}, DOI={10.1016/j.envres.2020.109435}, abstractNote={Among urban residents, increased contacts with nature are associated with reduced morbidity and mortality. The concept of allostatic load, a biomarker-based composite measure of physiological dysregulation, can be applied to study subclinical benefits of exposure, and to elucidate pathways leading to improved health. This research explored associations between residential vegetated land cover and an allostatic load index calculated using the statistical distance measure known as Mahalanobis distance. This cross-sectional population-based study involved 186 adult residents of the Durham-Chapel Hill, North Carolina metropolitan area. Measures of tree and grass cover within 500 m of residence were derived from the U.S. Environmental Protection Agency's EnviroAtlas land cover database. Fifteen biomarkers of immune, neuroendocrine, and metabolic functions were analyzed in serum samples. Regression analysis was conducted using generalized additive models with thin-plate spline functions of geographic coordinates, adjusting for modelled traffic air pollution from local sources and sociodemographic covariates. The second and third tertiles of distance-weighted tree cover were associated with 14% (95% Confidence Limits 20%; 8%) and 15% (21%; 8%) reduction in adjusted median allostatic load, respectively, compared to the first tertile. The same tertiles of tree cover were also associated with 0.16 (0.03; 0.76) and 0.04 (0.01; 0.35) adjusted odds ratios of having allostatic load index above the 90th percentile of the sample distribution. Grass cover was inversely correlated with tree cover and was not associated with reduced allostatic load. Subclinical beneficial health effects of green spaces demonstrated in this study are consistent with reduced susceptibility to acute environmental and social stressors, and reduced risks of morbidity and mortality.}, journal={Environmental Research}, year={2020}, month={Apr} }
 @article{styles_converse_griffin_wade_klein_nylander-french_stewart_sams_hudgens_egorov_2020, title={Human Cytomegalovirus Infections Are Associated With Elevated Biomarkers of Vascular Injury}, volume={10}, url={https://www.frontiersin.org/article/10.3389/fcimb.2020.00334}, DOI={10.3389/fcimb.2020.00334}, abstractNote={Background: Human cytomegalovirus (HCMV) infects ~50% of adults in the United States. HCMV infections may cause vascular inflammation leading to cardiovascular disease, but the existing evidence is inconsistent. Objective: We investigated demographic predictors of HCMV infection and explored associations between HCMV infection status, the intensity of anti-HCMV Immunoglobulin G (IgG) antibody response, and biomarkers of inflammation and endothelial function which are known predictors of cardiovascular disease. Methods: We conducted a cross-sectional study of 694 adults residing in the Raleigh-Durham-Chapel Hill, NC metropolitan area. Serum samples were tested for IgG antibody response to HCMV, and for biomarkers of vascular injury including soluble intercellular adhesion molecule 1 (sICAM-1), soluble vascular cell adhesion molecule 1 (sVCAM-1), C-reactive protein (CRP), and serum amyloid A (SAA). Associations between HCMV and biomarker levels were analyzed using two approaches with HCMV serostatus modeled as a binary variable and as an ordinal variable with five categories comprised of seronegative individuals and quartiles of anti-HCMV antibody responses in seropositive individuals. Results: HCMV seroprevalence in the study population was 56%. Increased body mass index, increased age, female gender, racial/ethnic minority status, and current smoking were significantly associated with HCMV seropositivity in a multivariate regression analysis. HCMV seropositivity was also associated with 9% (95% confidence interval 4–15%) and 20% (0.3–44%) increases in median levels of sICAM-1 and CRP, respectively, after adjusting for covariates. The association between HCMV seropositivity and median levels of sVCAM-1 and SAA were positive but not statistically significant. Significant positive associations were observed between the intensity of anti-HCMV IgG responses and levels of sICAM-1 and sVCAM-1 (p-values 0.0008 and 0.04 for linear trend, respectively). To our knowledge, this is the first epidemiological study to show a relationship between anti-HCMV IgG responses and vascular injury biomarkers sICAM-1 and sVCAM-1 in the general population. Conclusion: HCMV infections are associated with vascular injury and inflammation biomarkers in adult residents of North Carolina.}, journal={Frontiers in Cellular and Infection Microbiology}, author={Styles, Jennifer N. and Converse, Reagan R. and Griffin, Shannon M. and Wade, Timothy J. and Klein, Elizabeth and Nylander-French, Leena A. and Stewart, Jill R. and Sams, Elizabeth and Hudgens, Edward and Egorov, Andrey I.}, year={2020}, pages={334} }
 @inproceedings{zoss_klein_wilkinson saldaña_2020, title={Using Wax and Jekyll to build minimal digital projects}, author={Zoss, A. and Klein, J. and Wilkinson Saldaña, C.}, year={2020}, month={Nov} }
 @inproceedings{goldstein_sayedahmed_mohanty_klein_2020, title={Visualizing the Twitter record from Hurricane Irma in Florida to investigate coastal storm impacts}, author={Goldstein, E. and Sayedahmed, S. and Mohanty, S. and Klein, J.}, year={2020}, month={Feb} }
 @inproceedings{klein_2019, title={Behind the scenes: Metadata and data visualization}, author={Klein, J.}, year={2019}, month={Aug} }
 @inproceedings{klein_2019, title={But I'm not a GIS person! Teaching introductory GIS programming through an interactive web map workshop}, author={Klein, J.}, year={2019}, month={Feb} }
 @inproceedings{klein_murphy_2019, title={Customizable Leaflet Maps}, author={Klein, J. and Murphy, M.}, year={2019}, month={May} }
 @inproceedings{bird_crumpton_klein_2019, title={Data librarianship at UNC Greensboro}, author={Bird, N. and Crumpton, M. and Klein, J.}, year={2019}, month={Oct} }
 @article{egorov_griffin_styles_sams_hudgens_klein_wickersham_ritter_jackson_wade_2019, title={Greater residential tree-cover and time spent outdoors are associated with reduced allostatic load in residents of central North Carolina}, volume={3}, DOI={10.1097/01.ee9.0000606896.31619.ea}, abstractNote={OPS 28: Green space and biomarkers, Room 417, Floor 4, August 27, 2019, 4:30 PM - 5:30 PM Background. Greater vegetated land cover in urban areas has been linked to reduced morbidity and mortality. Our previous research showed that residential vegetated land cover was associated with reduced allostatic load, a biomarker-based measure of chronic stress-related physiological dysregulation. The objectives of the current study are to further explore pathways linking contacts with nature to health benefits. Methods. This cross-sectional study involved 335 adults residing in the Raleigh-Durham-Chapel Hill, NC urban area. Weighted measures of tree cover within 500 m of residence were estimated allowing for an exponential decay with distance. Blood samples were tested for biomarkers of immune, neuroendocrine, and metabolic functions. Allostatic load indices were estimated as sums of biomarkers dichotomized at distribution-based cut-offs. Regression models adjusted for socio-demographic and behavioral covariates, body mass index, waist-to-hip ratio, sleep problems, and cytomegalovirus seropositivity. Results. An allostatic load index based on inflammation biomarkers (interferon (IFN)-γ, interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10, IL-17, tumor necrosis factor (TNF)-α, C-reactive protein, myeloperoxidase, serum amyloid A, chromogranin A, vascular cell adhesion molecule (VCAM)-1, intracellular adhesion molecule (ICAM)-1, eosinophils, monocytes, and neutrophils) was reduced by 17% (5%; 28%) per interquartile range increase in tree cover in participants who reported spending at least 30 min per day outdoors (N=271). There was no effect in individuals spending less than 30 min per day outdoors. In a sub-population with tree cover above the median, time spent outdoors was linked with allostatic load reduction by 62% (33%; 79%) in individuals spending 3 hours per day or more, 43% (19%; 60%) for 1 to 3 hours group, and 37% (12%; 54%) for 0.5 to 1-hour group compared to controls spending less than 30 min outdoors. Conclusions. Exposure to the natural living environment in residential settings may alleviate physiological dysregulation and reduce chronic inflammation. This abstract does not represent EPA policy.}, journal={Environmental Epidemiology}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Egorov, A and Griffin, S and Styles, J and Sams, E and Hudgens, E and Klein, E and Wickersham, L and Ritter, R and Jackson, L and Wade, T}, year={2019}, month={Oct}, pages={108} }
 @article{klein_2019, title={New maps and cartographic materials}, volume={40}, ISSN={1943-6548}, url={https://www.ala.org/rt/sites/ala.org.rt/files/content/publicationsab/baseline/BL-40-2.pdf}, number={2}, journal={base line, a newsletter of the Map and Geospatial Information Round Table}, publisher={American Library Association}, author={Klein, J.}, year={2019}, month={Apr}, pages={21–29} }
 @inproceedings{carlton_klein_2019, title={Two birds one stone: Supporting data literacy and encouraging civic engagement using framework-inspired library programming}, author={Carlton, M. and Klein, J.}, year={2019}, month={Oct} }
 @article{egorov_converse_griffin_styles_klein_sams_hudgens_wade_2018, title={Environmental risk factors for Toxoplasma gondii infections and the impact of latent infections on allostatic load in residents of Central North Carolina}, volume={18}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85052325391&partnerID=MN8TOARS}, DOI={10.1186/s12879-018-3343-y}, abstractNote={Toxoplasma gondii infection can be acquired through ingestion of infectious tissue cysts in undercooked meat or environmental oocysts excreted by cats. This cross-sectional study assessed environmental risk factors for T. gondii infections and an association between latent infections and a measure of physiologic dysregulation known as allostatic load.Serum samples from 206 adults in the Durham-Chapel Hill, North Carolina area were tested for immunoglobulin (IgG) responses to T. gondii using commercial ELISA kits. Allostatic load was estimated as a sum of 15 serum biomarkers of metabolic, neuroendocrine and immune functions dichotomized at distribution-based cutoffs. Vegetated land cover within 500 m of residences was estimated using 1 m resolution data from US EPA's EnviroAtlas.Handling soil with bare hands at least weekly and currently owning a cat were associated with 5.3 (95% confidence limits 1.4; 20.7) and 10.0 (2.0; 50.6) adjusted odds ratios (aOR) of T. gondii seropositivity, respectively. There was also a significant positive interaction effect of handling soil and owning cats on seropositivity. An interquartile range increase in weighted mean vegetated land cover within 500 m of residence was associated with 3.7 (1.5; 9.1) aOR of T. gondii seropositivity. Greater age and consumption of undercooked pork were other significant predictors of seropositivity. In turn, T. gondii seropositivity was associated with 61% (13%; 130%) greater adjusted mean allostatic load compared to seronegative individuals. In contrast, greater vegetated land cover around residence was associated with significantly reduced allostatic load in both seronegative (p < 0.0001) and seropositive (p = 0.004) individuals.Residents of greener areas may be at a higher risk of acquiring T. gondii infections through inadvertent ingestion of soil contaminated with cat feces. T. gondii infections may partially offset health benefits of exposure to the natural living environment.}, number={1}, journal={BMC Infectious Diseases}, author={Egorov, A.I. and Converse, R. and Griffin, S.M. and Styles, J. and Klein, E. and Sams, E. and Hudgens, E. and Wade, T.J.}, year={2018} }
 @article{egorov_griffin_converse_styles_sams_klein_jackson_wade_2018, title={Greater Residential Tree Cover Is Associated with Reduced Stress-Related Physiological Dysregulation in Residents of Central North Carolina}, volume={2018}, DOI={10.1289/isesisee.2018.o04.02.21}, abstractNote={Background: Urban green spaces have been linked to reduced morbidity and mortality. Alleviation of chronic stress is a hypothesized mechanism of these salutogenic effects. Our previous research showed that greater residential greenness was associated with lower allostatic load suggesting reduced physiologic dysregulation. The objective of this analysis was to assess the effects of different vegetation types on the distribution of allostatic load values.Methods: This cross-sectional study involved 186 adult residents of the Durham-Chapel Hill, NC urban area. High-resolution metrics of tree and grass cover within 500 m of each residence were produced using US EPA EnviroAtlas database. Weighted tree cover measures were produced allowing for an exponential decay of effect with distance from residence. Serum samples were tested for fifteen biomarkers of neuroendocrine, metabolic, and immune functions. Allostatic load was estimated as a standardized distance from the center of a joint multivariate distribution of biomarkers (Mahalanobis distance). Statistical analysis involved two stages: (1) generalized additive regression models of allostatic load on socio-demographic covariates and a thin plate smoothing function of geographic coordinates; and (2) quantile regression of residuals from the 1st stage on a weighted proportion of tree or grass cover.Results: Individuals in the 3rd tertile of tree cover distribution compared to individuals in the 1st tertile had 8% (1%; 15%), 12% (4%; 20%), and 23% (12%; 33%) lower predicted values of the 50th, 75th, and 90th percentiles of the marginal distribution of allostatic load respectively. Grass cover was not a significant predictor of allostatic load.Conclusions: Trees near residence may help to alleviate chronic stress preventing stress-related physiological dysregulation (allostatic load). Health benefits may be stronger in individuals with greater chronic stress. This abstract does not represent EPA policy.}, number={1}, journal={ISEE Conference Abstracts}, publisher={Environmental Health Perspectives}, author={Egorov, Andrey and Griffin, Shannon and Converse, Reagan and Styles, Jennifer and Sams, Elizabeth and Klein, Elizabeth and Jackson, Laura and Wade, Timothy}, year={2018}, month={Sep} }