@article{reed_reiskind_reiskind_2022, title={Life-history stage and the population genetics of the tiger mosquito Aedes albopictus at a fine spatial scale}, volume={10}, ISSN={["1365-2915"]}, url={http://dx.doi.org/10.1111/mve.12618}, DOI={10.1111/mve.12618}, abstractNote={Abstract}, journal={MEDICAL AND VETERINARY ENTOMOLOGY}, publisher={Wiley}, author={Reed, Emily M. X. and Reiskind, Michael H. and Reiskind, Martha O. Burford}, year={2022}, month={Oct} } @article{wallace_reed_aguilar_reiskind_2022, title={Resolving the phylogenetic relationship among recently diverged members of the rockfish subgenus Sebastosomus}, volume={173}, ISSN={["1095-9513"]}, DOI={10.1016/j.ympev.2022.107515}, abstractNote={Rapid speciation is an important aspect of adaptive radiations, but can obfuscate phylogenetic relationships among taxa. For recent radiations, there are challenges to reconstructing the relationships among the species due to often shorter branch lengths. Resolution of these relationships is further confounded when studies only use a few genetic markers. Double digest restriction-site associated DNA sequencing (ddRADseq) is a method of next generation sequencing that identifies many single nucleotide polymorphisms (SNPs) throughout the genome. This increases statistical power to resolve close phylogenetic relationships like those found within an adaptive radiation. We used this approach to understand the evolutionary history of the rockfishes of the genus Sebastes, which experienced an adaptive radiation between 3 and 5 mya. Here, we reconstructed the phylogenetic relationships among six species of rockfish within the subgenus Sebastosomus using over 11,600 SNPs. This reconstruction includes the two recently diverged species, Sebastes mystinus and S. diaconus, that were first described genetically in 2008 using mtDNA control region sequence data and six microsatellite loci. We confirmed the relationship of these cryptic species as sister-taxa and found evidence that S. melanops and S. flavidus were also sister-taxa. The latter contradicts prior studies but is supported by our reconstruction using nuclear DNA and measures of genetic differentiation tests and a discriminant analysis of principal components. The relationships between the species of Sebastosomus are further supported by morphological, biological, and ecological justifications.}, journal={MOLECULAR PHYLOGENETICS AND EVOLUTION}, author={Wallace, E. N. and Reed, E. M. X. and Aguilar, A. and Reiskind, M. O. Burford}, year={2022}, month={Aug} } @article{reiskind_styers_hayes_richards_doyle_reed_hollingsworth_byrd_2020, title={Short-Term, Large-Area Survey of ContainerAedes spp. (Diptera: Culicidae): Presence and Abundance is Associated with Fine-scale Landscape Factors in North Carolina, USA}, volume={14}, ISSN={["1178-6302"]}, url={http://dx.doi.org/10.1177/1178630220952806}, DOI={10.1177/1178630220952806}, abstractNote={ Container Aedes mosquitoes are responsible for the transmission of anthroponotic and zoonotic viruses to people. The surveillance and control of these mosquitoes is an important part of public health protection and prevention of mosquito-borne disease. In this study, we surveyed 327 sites over 2 weeks in late June and early July in 2017 in North Carolina, USA for the presence and abundance of Aedes spp. eggs in an effort to better target potential Ae. aegypti collections. We examined the ability of 2 types of landscape data, Light Detection And Ranging (LIDAR) and National Land Cover Database (NLCD) to explain the presence and abundance of eggs using principal component analysis to deal with collinearity, followed by generalized linear regression. We explained variation of both egg presence and abundance for Aedes albopictus (Skuse) and Aedes triseriatus (Say) using both NLCD and LIDAR data. However, the ability to make robust predictions was limited by variation in the data. Increased sampling time and better landscape data would likely improve the predictive ability of our models, as would a better understanding of oviposition behavior. }, journal={ENVIRONMENTAL HEALTH INSIGHTS}, publisher={SAGE Publications}, author={Reiskind, Michael H. and Styers, Diane M. and Hayes, Isaac and Richards, Stephanie L. and Doyle, Michael S. and Reed, Emily M. X. and Hollingsworth, Brandon and Byrd, Brian D.}, year={2020}, month={Sep} } @article{reed_byrd_richards_eckardt_williams_reiskind_2018, title={A Statewide Survey of ContainerAedesMosquitoes (Diptera: Culicidae) in North Carolina, 2016: A Multiagency Surveillance Response to Zika Using Ovitraps}, volume={56}, ISSN={0022-2585 1938-2928}, url={http://dx.doi.org/10.1093/jme/tjy190}, DOI={10.1093/jme/tjy190}, abstractNote={Abstract Native and invasive container-inhabiting Aedes mosquitoes (Diptera: Culicidae) play important roles in the transmission of endemic and traveler-introduced arboviruses in the United States. In response to the emergence of Zika virus into the Americas, we surveyed the distribution of container Aedes spp. of public health importance within North Carolina during 2016 using ovitraps. A seasonal survey was conducted in 18 counties from the mountains to the coast to identify species incriminated in the transmission of chikungunya, dengue, La Crosse, yellow fever, and Zika viruses. Multiple local, state, and federal agencies participated in the study and submitted more than 3,600 ovistrips. Aedes albopictus (Skuse) (81.4%, n = 54,458) was the most common and widespread species found in this survey, followed by Aedes triseriatus (Say) (10.7%, n = 7,169) and Aedes japonicus (Theobald) (7.9%, n = 5,262). We did not find Aedes aegypti and rarely found Aedes hendersoni (Cockerell). We assessed broad-scale climatic and other factors and determined that longitude, elevation, rainfall, and temperature had significant effects on explaining the variation in presence, abundance, and phenology of container Aedes in North Carolina. However, much of the variation in these outcomes was not explained at this coarse scale and may benefit from finer-scale analyses. These efforts represent the largest ovitrap survey ever conducted in the state.}, number={2}, journal={Journal of Medical Entomology}, publisher={Oxford University Press (OUP)}, author={Reed, Emily M X and Byrd, Brian D and Richards, Stephanie L and Eckardt, Megan and Williams, Carl and Reiskind, Michael H}, year={2018}, month={Oct}, pages={483–490} }