Works (56)

Updated: April 3rd, 2024 20:15

2023 review

Tolyporphins-Exotic Tetrapyrrole Pigments in a Cyanobacterium-A Review

[Review of ]. MOLECULES, 28(16).

By: K. Nguyen n, Y. Zhang n, Q. Liu n, R. Zhang n, X. Jin n, M. Taniguchi n, E. Miller n, J. Lindsey n

author keywords: bacteriochlorin; bioinformatics; biosynthesis; chlorophyll; cyanobacteria; field biology; gene cluster; genomics; natural products; tolyporphins
TL;DR: The identification of putative biosynthetic gene clusters in the HT-58-2 cyanobacterial genome and accompanying studies suggest a new biosynthesis paradigm in the tetrapyrrole arena, which stands alone in the large pigments-of-life family. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: October 2, 2023

2021 journal article

Identification of Putative Biosynthetic Gene Clusters for Tolyporphins in Multiple Filamentous Cyanobacteria

LIFE-BASEL, 11(8).

By: X. Jin n, Y. Zhang n, R. Zhang n, K. Nguyen n, J. Lindsey n & E. Miller n

author keywords: tolyporphins; tetrapyrroles; cyanobacteria; biosynthetic gene cluster; Brasilonema; Nostoc; Oculatella
TL;DR: Phylogenetic analysis based on 16S rRNA and a common component of the BGCs, TolD, points to a close evolutionary history between each strain and their respective tolyporphins BGC, and a collection of candidate cyanobacteria for genetic and biochemical analysis pertaining to these unusual tetrapyrrole macrocycles. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: September 7, 2021

2021 journal article

Natural Product Gene Clusters in the Filamentous Nostocales Cyanobacterium HT-58-2

LIFE-BASEL, 11(4).

By: X. Jin n, E. Miller n & J. Lindsey n

author keywords: anatoxin-a; homoanatoxin-a; hapalosin; heterocyst glycolipids; natural products; secondary metabolites; shinorine; tolypodiols; tolyporphins
TL;DR: The results point to the rich biosynthetic capacity of the HT-58-2 cyanobacterium beyond the production of tolyporphins and tolypodiols, as well as the biosynthesis of other natural products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: May 17, 2021

2019 journal article

Cellular localization of tolyporphins, unusual tetrapyrroles, in a microbial photosynthetic community determined using hyperspectral confocal fluorescence microscopy

Photosynthesis Research, 141(3), 259–271.

By: M. Barnhart-Dailey*, Y. Zhang n, R. Zhang n, S. Anthony*, J. Aaron*, E. Miller n, J. Lindsey n, J. Timlin*

author keywords: Bacteriochlorin; Chlorophyll; Cyanobacteria; Membrane; Multivariate curve resolution
MeSH headings : Adaptation, Physiological; Bacteriochlorophyll A / chemistry; Chlorophyll A / chemistry; Cyanobacteria / metabolism; Cyanobacteria / ultrastructure; Darkness; Microscopy, Confocal; Microscopy, Fluorescence; Photosynthesis; Porphyrins / chemistry; Porphyrins / metabolism; Tetrapyrroles / chemistry; Tetrapyrroles / metabolism
TL;DR: This work quantifies the relative abundance and envelope localization of tolyporphins in single cells, and illustrates the ability to identify novel tetrapyrroles in the presence of chlorophyll a in a photosynthetic microorganism within a non-axenic culture. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: September 23, 2019

2018 journal article

Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium

Microbiology, 164(10), 1229–1239.

By: R. Hughes n, X. Jin n, Y. Zhang n, R. Zhang n, S. Tran n, P. Williams*, J. Lindsey n, E. Miller n

author keywords: alphaproteobacteria; fluorescence in situ hybridization (FISH); tolyporphins; bacteriochlorin; photosynthetic gene cluster
MeSH headings : Alphaproteobacteria / classification; Alphaproteobacteria / genetics; Alphaproteobacteria / metabolism; Alphaproteobacteria / physiology; Bacterial Proteins / genetics; Base Sequence; Chromosome Mapping; Cyanobacteria / metabolism; DNA, Bacterial / genetics; Genome, Bacterial / genetics; In Situ Hybridization, Fluorescence; Microbial Consortia; Phylogeny; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR: The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT- 58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID, Crossref
Added: January 7, 2019

2017 journal article

An inclusive Research Education Community (iREC): Impact of the SEA-PHAGES program on research outcomes and student learning

Proceedings of the National Academy of Sciences, 114(51), 13531–13536.

By: D. Hanauer*, M. Graham*, L. Betancur*, A. Bobrownicki*, S. Cresawn*, R. Garlena*, D. Jacobs-Sera*, N. Kaufmann* ...

author keywords: bacteriophage; genomics; science education; evolution; assessment
MeSH headings : Biomedical Research / education; Biomedical Research / standards; Education, Medical, Undergraduate / methods; Education, Medical, Undergraduate / standards; Female; Humans; Learning; Male; Program Evaluation; Teaching; Universities / standards; Young Adult
TL;DR: The SEA-PHAGES students show strong gains correlated with persistence relative to those in traditional laboratory courses regardless of academic, ethnic, gender, and socioeconomic profiles. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

2017 journal article

Genome Sequence and Composition of a Tolyporphin-Producing Cyanobacterium-Microbial Community

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(19).

By: R. Hughes n, Y. Zhang n, R. Zhang n, P. Williams*, J. Lindsey n & E. Miller n

author keywords: cyanobacteria; genome analysis; phylogenetic analysis; tetrapyrroles; tolyporphin
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosynthetic Pathways; Cyanobacteria / chemistry; Cyanobacteria / genetics; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Genome, Bacterial; Metagenomics; Multigene Family; Phylogeny; Porphyrins / biosynthesis; Porphyrins / chemistry
TL;DR: It is found that the HT-58-2 culture is composed of the cyanobacterium and a community of associated bacteria, complicating the question of which organisms make tolyporphins, and an intriguing gene cluster that contains tetrapyrrole biosynthesis genes and a collection of unknown genes suggests a possible role in toly porphin biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Mass spectrometric detection of chlorophyll a and the tetrapyrrole secondary metabolite tolyporphin A in the filamentous cyanobacterium HT-58-2. Approaches to high-throughput screening of intact cyanobacteria

JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, 21(11), 759–768.

By: Y. Zhang n, R. Zhang n, M. Nazari n, M. Bagley n, E. Miller n, P. Williams*, D. Muddiman n, J. Lindsey n

author keywords: IR-MALDESI-FTMS; MS/MS; MALDI-TOF-MS; chlorophyll; bacteriochlorin; high-throughput screening
TL;DR: Examination by MALDI-TOF-MS of lipophilic crude extracts of small-scale HT-58-2 samples without chromatographic fractionation enabled semi-quantitation of tolyporphin A over a 41-day growth period. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Photophysical Characterization of the Naturally Occurring Dioxobacteriochlorin Tolyporphin A and Synthetic Oxobacteriochlorin Analogues

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 93(5), 1204–1215.

MeSH headings : Cyanobacteria / chemistry; Photochemistry; Porphyrins / chemistry; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet
TL;DR: The photophysical characterization is reported of tolyporphin A and two synthetic analogues, an oxobacteriochlorin and a dioxobacterium‐containing culture known as HT‐58‐2, which generally exhibits spectral and photophysical features resembling those of chlorophyll a. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Quantitation of Tolyporphins, Diverse Tetrapyrrole Secondary Metabolites with Chlorophyll-Like Absorption, from a Filamentous Cyanobacterium-Microbial Community

Phytochemical Analysis, 29(2), 205–216.

By: Y. Zhang n, R. Zhang n, R. Hughes n, J. Dai*, J. Gurr*, P. Williams*, E. Miller n, J. Lindsey n

author keywords: HPLC; mass spectrometry; absorption spectroscopy with multicomponent analysis; nitrogen deprivation; growth curve
MeSH headings : Chlorophyll / chemistry; Chlorophyll A; Chromatography, High Pressure Liquid / methods; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Porphyrins / analysis; Porphyrins / chemistry; Reference Standards; Reproducibility of Results; Spectrum Analysis / methods; Tetrapyrroles / analysis
TL;DR: The quantitative methods developed herein should facilitate investigation of the biosynthesis of tolyporphins (and other tetrapyrroles) as well as examination of other strains for production of tolporph Ins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2017 journal article

Vibrio Phage KVP40 Encodes a Functional NAD(+) Salvage Pathway

JOURNAL OF BACTERIOLOGY, 199(9).

By: J. Lee n, Z. Li n & E. Miller n

author keywords: NAD; bacteriophages; genomics; metabolism
MeSH headings : Bacteriophage T4 / genetics; Escherichia coli / genetics; Genomics; Metabolic Networks and Pathways / genetics; NAD / metabolism; Nicotinamide-Nucleotide Adenylyltransferase / genetics; Nicotinamide-Nucleotide Adenylyltransferase / metabolism; Nucleotides; Pyridines / chemistry; Pyrophosphatases / metabolism; Vibrio parahaemolyticus / virology
TL;DR: It is shown that phage KVP40 encodes a functional pyridine nucleotide scavenging pathway that is expressed during the metabolic period of the infection cycle, and the pathway is conserved in other large, dsDNA phages in which the two genes, nadV and natV, share an evolutionary history in their respective phage-host group. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Genome Sequence of Aeromicrobium erythreum NRRL B-3381, an Erythromycin-Producing Bacterium of the Nocardioidaceae

Genome Announcements, 4(2).

By: E. Harrell n & E. Miller n

TL;DR: Aeromicrobium erythreum NRRL B-3381 has a 3,629,239-bp circular genome that has 72% G+C content and provides resources for metabolic and combinatorial engineering of polyketides. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

2016 journal article

Paenibacillus larvae Phage Tripp Genome Has 378-Base-Pair Terminal Repeats

Genome Announcements, 4(1).

By: J. Abraham n, A. Bousquet n, E. Bruff n, N. Carson n, A. Clark n, A. Connell n, Z. Davis n, J. Dums n ...

TL;DR: Paenibacillus larvae bacteriophage Tripp was isolated from an American foulbrood diseased honey bee hive in North Carolina, USA and is currently unique in Genbank. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

2015 report

Divavirus taxonomic bacteriophage group infecting Paenibacillus larvae

International Committee on the Taxonomy of Viruses.

By: E. Miller, J. Whittman, A. Kropinski & E. Areaenssesn

Source: NC State University Libraries
Added: February 6, 2021

2015 journal article

Genome Sequences of Six Paenibacillus larvae Siphoviridae Phages

Genome Announcements, 3(3).

By: S. Carson n, E. Bruff, W. DeFoor, J. Dums, A. Groth, T. Hatfield, A. Iyer, K. Joshi ...

TL;DR: Six sequenced and annotated genomes of Paenibacillus larvae phages isolated from the combs of American foulbrood-diseased beehives show Phage Lily is most divergent from Diva, Rani, Redbud, Shelly, and Sitara. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

2015 journal article

Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity

ELife, 4.

By: W. Pope*, C. Bowman*, D. Russell*, D. Jacobs-Sera*, D. Asai*, S. Cresawn*, W. Jacobs*, R. Hendrix*, J. Lawrence*, G. Hatfull*

MeSH headings : Biomedical Research / ethics; Cooperative Behavior; DNA, Viral / genetics; Gene Flow; Genetic Variation; Genome, Viral; High-Throughput Nucleotide Sequencing; Information Dissemination; Mosaicism; Mycobacteriophages / classification; Mycobacteriophages / genetics; Mycobacterium smegmatis / virology; Phylogeny; Phylogeography; Workforce
TL;DR: To understand the population structure of phages infecting a single host strain, 627 phages of Mycobacterium smegmatis were isolated, sequenced, and compared to reveal a continuum of genetic diversity, albeit with uneven representation of different phages. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

2014 conference paper

Modeling ribosome dynamics to optimize heterologous protein production in escherichia coli

2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP). Presented at the 2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP).

By: S. Vu n, A. Belloti n, C. Gabriel n, H. Brochu n, E. Miller n, D. Bitzer n, M. Vouk n

Event: 2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP)

TL;DR: A free-energy based model of translation elongation to predict and optimize genes for expression in E. coli is presented and protein yield of optimized genes showed increase from their wildtype levels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, ORCID
Added: January 7, 2020

2013 journal article

Introducing primary scientific literature to first-year undergraduate researchers

Council on Undergraduate Research on the Web, 34(4), 17–22.

By: S. Carson & E. Miller

Source: NC State University Libraries
Added: February 6, 2021

2010 article

Bacteriophage T4 and its relatives

Karam, J. D., & Miller, E. S. (2010, October 28). VIROLOGY JOURNAL, Vol. 7.

By: J. Karam* & E. Miller n

TL;DR: The review series to be published in Virology Journal will emphasize advances and seminal discoveries in four major areas of T4 research: Genomics, Gene Expression, DNA Replication and Phage Morphogenesis. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 review

Genomes of the T4-related bacteriophages as windows on microbial genome evolution

[Review of ]. VIROLOGY JOURNAL, 7.

By: V. Petrov, S. Ratnayaka*, J. Nolan*, E. Miller n & J. Karam*

MeSH headings : Bacteriophages / genetics; Biological Evolution; DNA, Viral / chemistry; DNA, Viral / genetics; Evolution, Molecular; Genetic Variation; Genome, Viral; Molecular Sequence Data; Sequence Analysis, DNA
TL;DR: The high degree of genetic heterogeneity that exists outside of this shared core suggests that horizontal DNA transfer involving many genetic sources has played a major role in diversification of the T4-related phages and their spread to a wide spectrum of bacterial species domains in evolution. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 review

Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation

[Review of ]. VIROLOGY JOURNAL, 7.

By: M. Uzan* & E. Miller n

MeSH headings : Bacteriophage T4 / genetics; Gene Expression Regulation; Protein Biosynthesis; RNA Processing, Post-Transcriptional; RNA Stability / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism
TL;DR: The past 10 - 15 year literature on RNA-protein interactions with T4 and some of its related phages is compiled, with particular focus on advances in mRNA decay and processing, and on translational repression. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 journal article

Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases

TRANSGENIC RESEARCH, 17(1), 47–57.

By: S. Dong n, H. Shew n, L. Tredway n, J. Lu n, E. Sivamani n, E. Miller n, R. Qu n

author keywords: fungal resistance; Magnaporthe grisea; Rhizoctonia solani; T4 lysozyme; tall fescue
MeSH headings : Bacteriophage T4 / enzymology; Bacteriophage T4 / genetics; Cloning, Molecular; Festuca / enzymology; Festuca / genetics; Festuca / microbiology; Gene Expression; Genes, Viral; Genetic Vectors; Magnaporthe / pathogenicity; Muramidase / genetics; Plant Diseases / genetics; Plant Diseases / microbiology; Plants, Genetically Modified; Rhizobium / genetics; Rhizoctonia / pathogenicity
TL;DR: It is demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants, which may have wide applications in engineered fungal disease resistance in various crops. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

In vitro selection of phage RB69 RegA RNA binding sites yields UAA repeats

VIROLOGY, 336(1), 26–36.

By: T. Dean n, S. Allen n & E. Miller n

author keywords: RNA SELEX; translation repressor; T4-type phage
MeSH headings : Binding Sites / genetics; Codon, Terminator / genetics; Directed Molecular Evolution; Electrophoretic Mobility Shift Assay; Genes, Viral / genetics; Protein Binding; RNA, Viral / genetics; RNA, Viral / metabolism; RNA-Binding Proteins / metabolism; Repetitive Sequences, Nucleic Acid / genetics; Repressor Proteins / metabolism; Viral Proteins / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2004 journal article

A family of anti-sigma(70) proteins in T4-type phages and bacteria that are similar to AsiA, a transcription inhibitor and co-activator of bacteriophage T4

JOURNAL OF MOLECULAR BIOLOGY, 344(5), 1183–1197.

By: M. Pineda*, B. Gregory*, B. Szczypinski n, K. Baxter*, A. Hochschild*, E. Miller n, D. Hinton*

author keywords: phage; AsiA; Rsd; AlgQ; transcription
MeSH headings : Amino Acid Sequence; Bacteriophage T4 / chemistry; Bacteriophage T4 / genetics; Cell Proliferation; DNA-Binding Proteins / metabolism; DNA-Directed RNA Polymerases / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Gene Expression Regulation; Holoenzymes / chemistry; Holoenzymes / metabolism; Molecular Sequence Data; Multigene Family; Multiprotein Complexes; Phenylalanine / genetics; Phenylalanine / metabolism; Protein Binding; Repressor Proteins / chemistry; Repressor Proteins / metabolism; Sequence Alignment; Sigma Factor / metabolism; Transcription Factors / metabolism; Transcription, Genetic; Two-Hybrid System Techniques; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: It is speculated that the phage AsiA orthologs, Rsd, and AlgQ are members of a related family in T4-type phage and bacteria, which interact similarly with primary sigma factors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2004 journal article

Characterization of bacteriophage KVP40 and T4 RNA ligase 2

VIROLOGY, 319(1), 141–151.

By: S. Yin*, C. Ho*, E. Miller n & S. Shuman*

author keywords: bacteriophage; KVP40; T4 RNA ligase 2
MeSH headings : Adenosine Monophosphate / metabolism; Adenosine Triphosphate / metabolism; Amino Acid Sequence; Bacteriophages / enzymology; Carbohydrates / analysis; Molecular Sequence Data; RNA / chemistry; RNA / metabolism; RNA Caps / chemistry; RNA Caps / metabolism; RNA Ligase (ATP) / classification; RNA Ligase (ATP) / genetics; RNA Ligase (ATP) / metabolism; Recombinant Proteins / metabolism; Sequence Alignment; Viral Proteins / classification; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: The ATP-dependent "capping" of RNA with AMP by Rnl2 is reminiscent of the capping of eukaryotic mRNA with GMP by GTP:RNA guanylyltransferase and suggests an evolutionary connection between bacteriophage Rnl 2 and eukARYotic RNA capping enzymes. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2003 review

Bacteriophage T4 genome

[Review of ]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 67(1), 86-+.

By: E. Miller n, E. Kutter*, G. Mosig*, F. Arisaka*, T. Kunisawa* & W. Ruger*

MeSH headings : Bacteriophage T4 / genetics; Bacteriophage T4 / pathogenicity; DNA Repair; DNA Replication; DNA, Viral / metabolism; Genome, Viral; Lysogeny; Models, Molecular; Oligonucleotide Array Sequence Analysis; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Viral / chemistry; Ribosomes / metabolism; Transcription, Genetic
TL;DR: T4 functional genomics will aid in the interpretation of these newly sequenced T4-related genomes and in broadening the understanding of the complex evolution and ecology of phages—the most abundant and among the most ancient biological entities on Earth. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2003 journal article

Complete genome sequence of the broad-host-range vibriophage KVP40: Comparative genomics of a T4-related bacteriophage

JOURNAL OF BACTERIOLOGY, 185(17), 5220–5233.

By: E. Miller n, J. Heidelberg, J. Eisen, W. Nelson, A. Durkin, A. Ciecko, T. Feldblyum, O. White ...

MeSH headings : Bacteriophage T4 / genetics; Genome, Viral; Genomics; Molecular Sequence Data; Myoviridae / genetics; Myoviridae / physiology; Sequence Analysis, DNA; Vibrio parahaemolyticus / virology; Viral Proteins / genetics
TL;DR: Features of the KVP40 genome that distinguish it from T4 are presented, as well as those, such as the replication and virion gene clusters, that are substantially conserved. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2000 journal article

Subtilisins of Bacillus spp. hydrolyze keratin and allow growth on feathers

CANADIAN JOURNAL OF MICROBIOLOGY, 46(11), 1004–1011.

By: K. Evans, J. Crowder & E. Miller*

author keywords: keratin hydrolysis; Bacillus; subtilisin; keratinase
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

1999 journal article

RNA-binding properties of in vitro expressed histidine-tagged RB69 RegA translational repressor protein

ANALYTICAL BIOCHEMISTRY, 269(1), 32–37.

By: S. Allen n & E. Miller n

MeSH headings : Bacteriophage T4 / genetics; Chromatography, Affinity / methods; Escherichia coli / metabolism; Histidine / genetics; Nickel / metabolism; Polymerase Chain Reaction / methods; Protein Biosynthesis; RNA / metabolism; RNA-Binding Proteins / biosynthesis; RNA-Binding Proteins / genetics; RNA-Binding Proteins / metabolism; Recombinant Proteins / metabolism; Repressor Proteins / biosynthesis; Repressor Proteins / genetics; Repressor Proteins / metabolism; Templates, Genetic; Viral Proteins / biosynthesis; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: In vitro synthesis of His-tagged proteins directly from linear PCR products provides a convenient and efficient system to study RegA and other interesting RNA-binding proteins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

1998 patent

DNA encoding Bacillus lichenformis PWD-1 keratinase

Washington, DC: U.S. Patent and Trademark Office.

By: J. Shih, X. Lin & E. Miller

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

An E-coli B mutation, rpoB5081, that prevents growth of phage T4 strains defective in host DNA degradation

FEMS Microbiology Letters, 157(1), 109–116.

By: E. Miller n, G. Shih n, S. Chang n & D. Ballard n

MeSH headings : Alleles; Bacteriophage T4 / growth & development; Chromosome Mapping; DNA Repair; DNA, Bacterial / biosynthesis; DNA, Bacterial / metabolism; DNA-Directed RNA Polymerases / chemistry; DNA-Directed RNA Polymerases / metabolism; Endonucleases / metabolism; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / virology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Mutation; Phenotype; Sequence Analysis, DNA; Transcription Factors / metabolism; Transcription, Genetic; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: An E. coli B Tab strain was isolated that restricts T4 denA (DNA endonuclease II) mutants at 37 degrees C and above, but is permissive for wild-type T4 at all temperatures examined, and is an important host for screening and mapping T 4 denA mutations. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

1997 article

Expression of the Bacillus licheniformis PWD-1 keratinase gene in B-subtilis

Lin, X., Wong, S. L., Miller, E. S., & Shih, J. C. H. (1997, August). JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, Vol. 19, pp. 134–138.

By: X. Lin n, S. Wong*, E. Miller n & J. Shih n

author keywords: keratinase; gene cloning; gene expression; Bacilli
MeSH headings : Bacillus / enzymology; Bacillus subtilis / genetics; Cloning, Molecular; Glucose / pharmacology; Peptide Hydrolases / biosynthesis; Peptide Hydrolases / genetics; Plasmids; Recombinant Proteins / biosynthesis
TL;DR: The kerA gene which encodes the enzyme keratinase was isolated from the feather-degrading bacterium Bacillus licheniformis PWD-1 and transformants and was further characterized when glucose or casamino acids were supplemented into the feather medium. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

1995 journal article

Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1.

Applied and Environmental Microbiology, 61(4), 1469–1474.

By: X. Lin n, D. Kelemen n, E. Miller n & J. Shih n

MeSH headings : Amino Acid Sequence; Animals; Bacillus / enzymology; Bacillus / genetics; Bacteriophage lambda / genetics; Base Sequence; Biodegradation, Environmental; DNA Primers / genetics; DNA, Bacterial / genetics; Feathers; Gene Expression Regulation, Bacterial; Gene Library; Genes, Bacterial; Molecular Sequence Data; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Phenylmethylsulfonyl Fluoride / pharmacology; Polymerase Chain Reaction / methods; Poultry; Sequence Homology, Amino Acid; Subtilisins / genetics
TL;DR: NorthernRNA analysis demonstrates that transcriptional regulation controls kerA expression on different growth media and deduced amino acid sequences indicate only three amino acid differences between the two mature proteases. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1995 journal article

RNA-protein interactions of the bacteriophage RB69 RegA translational repressor protein

Nucleic Acids Symposium Series, 33, 256–257.

By: C. Jozwik & E. Miller

Source: NC State University Libraries
Added: January 23, 2021

1994 chapter

Control of translation initiation: mRNA structure and protein repressors

In J. D. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular Biology of Bacteriophage T4 (pp. 193–205). Washington, D.C: ASM Press.

By: E. Miller, J. Karam & E. Spicer

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

Detection of plasmid encoded T4 proteins: radiolabeling and biological activity

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.

By: E. Miller & J. Karam

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

Experiments in T4 genetics

In J. D. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular Biology of Bacteriophage T4 (pp. 421–483). Washington, D.C: ASM Press.

By: K. Carlson, E. Miller & J. Karam

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

General considerations for strain construction

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4 (pp. 438–441). Washington, D.C.: ASM Press.

By: K. Carlson & E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

General procedures for manipulating bacteriophage T4

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.

By: K. Carlson & E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

Large scale isolation of mRNA from T4-infected cells

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.

By: J. Karam, T. Hsu & E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 book

Molecular Biology of Bacteriophage T4

Washington, D.C: ASM Press.

By: J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

PCR amplification of DNA from T4 plaques

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.

By: C. Jozwik & E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

Quantitation of T4 DNA synthesis by radiolabelling

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.

By: E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

Radiolabelling of T4 proteins and their analysis by gel electrophoresis

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.

By: E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1994 chapter

Rapid screening of phage progeny from multiple-factor crosses

In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hill, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4 (pp. 442–443). Washington, D.C.: ASM Press.

By: H. Kreuzer & E. Miller

Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hill, F. Eiserling, L. Black, E. Spicer ...

Source: NC State University Libraries
Added: January 28, 2021

1992 journal article

Regions of bacteriophage T4 and RB69 RegA translational repressor proteins that determine RNA-binding specificity.

Proceedings of the National Academy of Sciences, 89(11), 5053–5057.

By: C. Jozwik n & E. Miller n

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Gene Expression Regulation, Viral; Genes, Viral; Molecular Sequence Data; Mutation; Protein Biosynthesis; RNA, Messenger / metabolism; RNA-Binding Proteins / chemistry; RNA-Binding Proteins / metabolism; Repressor Proteins / chemistry; Sequence Alignment; Structure-Activity Relationship; T-Phages / genetics; Viral Proteins / chemistry; Viral Structural Proteins / genetics
Sources: Crossref, ORCID
Added: January 19, 2020

1991 journal article

Cloning vectors, mutagenesis, and gene disruption (ermR) for the erythromycin-producing bacterium Aeromicrobium erythreum.

Applied and Environmental Microbiology, 57(9), 2758–2761.

By: E. Miller n

MeSH headings : Amino Acids / physiology; Chromosomes, Bacterial; Cloning, Molecular; DNA Replication; Erythromycin / biosynthesis; Genes, Bacterial; Genetic Vectors; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Mutagenesis; Plasmids; Recombination, Genetic
TL;DR: P Phenotypic characterizations demonstrated that ermR is the sole determinant of macrolide antibiotic resistance in A. erythreum. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1991 journal article

Description of the Erythromycin-Producing Bacterium Arthrobacter sp. Strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov.

International Journal of Systematic Bacteriology, 41(3), 363–368.

By: E. Miller n, C. Woese* & S. Brenner*

MeSH headings : Amino Acids / analysis; Arthrobacter / classification; Arthrobacter / metabolism; Bacteria, Aerobic / classification; Bacteria, Aerobic / metabolism; Base Composition; Base Sequence; Erythromycin / metabolism; Fatty Acids / analysis; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / metabolism; Microscopy, Electron; Molecular Sequence Data; Peptidoglycan / chemistry; Phenotype; RNA, Ribosomal, 16S / genetics; Sequence Homology, Nucleic Acid; Soil Microbiology; Vitamin K / analysis
TL;DR: The principal long-chain fatty acids which were identified that distinguished strain NRRL B-3381T from related G + C-rich bacteria were 10-methyloctadecanoic (tuberculosteric), octadecenoic, and hexadecanoIC acids, which form the basis for the recommendation that strain NR RL B- 3381 should be the type strain of a new taxon, for which the name Aeromicrobium erythreum is proposed. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1990 journal article

Sequence analysis of conserved regA and variable orf43.1 genes in T4-like bacteriophages.

Journal of Bacteriology, 172(9), 5180–5186.

By: E. Miller n & C. Jozwik n

MeSH headings : Amino Acid Sequence; Base Sequence; Coliphages / genetics; DNA Transposable Elements; Escherichia coli / genetics; Genes, Viral; Molecular Sequence Data; Oligonucleotide Probes; T-Phages / genetics; Viral Proteins / genetics
TL;DR: Bacteriophage T4 RegA protein is a translational repressor of several phage mRNAs in the T4-related phages examined, regA nucleotide sequences are highly conserved and the inferred amino acid sequences are identical. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1987 journal article

Translational repression: Biological activity of plasmid-encoded bacteriophage T4 RegA protein

Journal of Molecular Biology, 194(3), 397–410.

By: E. Miller*, J. Karam*, M. Dawson*, M. Trojanowska*, P. Gauss* & L. Gold*

MeSH headings : Base Sequence; Binding Sites; DNA, Viral / genetics; Gene Expression Regulation; Plasmids; Protein Biosynthesis; Repressor Proteins / genetics; T-Phages / genetics; Transcription, Genetic; Viral Proteins / genetics
TL;DR: Cloned wild-type and mutant alleles of the T4 regA gene under control of the heat-inducible, plasmid-borne leftward promoter of phage lambda resulted in the synthesis of a protein that closely resembled phage-encoded RegA protein in biological properties. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1985 journal article

Bacteriophage T4 RegA protein. Purification of a translational repressor

Journal of Biological Chemistry, 260(24), 13053–13059.

By: E. Miller*, R. Winter*, K. Campbell*, S. Power* & L. Gold*

TL;DR: The bacteriophage T4 regA protein translationally regulates its own synthesis and the synthesis of several other T4 early proteins and the mechanism of translational regulation is studied in relation to the mechanism by which the protein recognizes its mRNA targets. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: January 29, 2021

1984 journal article

Cloning and characterization of gdhA, the structural gene for glutamate dehydrogenase of Salmonella typhimurium.

Journal of Bacteriology, 157(1), 171–178.

By: E. Miller* & J. Brenchley

MeSH headings : Cloning, Molecular; DNA, Bacterial / genetics; Escherichia coli / enzymology; Escherichia coli / genetics; Genes; Genes, Bacterial; Genetic Complementation Test; Glutamate Dehydrogenase / genetics; Glutamate Synthase / genetics; Mutation; Operon; Plasmids; Salmonella typhimurium / enzymology; Salmonella typhimurium / genetics
TL;DR: S. typhimurium gdhA DNA was shown to hybridize to single restriction fragments of chromosomes from other enteric bacteria and from Saccharomyces cerevisiae, and was regulated in both organisms. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1984 journal article

The bacteriophage T4regAgene: primary sequence of a translational repressor

Nucleic Acids Research, 12(15), 5979–5993.

By: M. Trojanowska, E. Miller*, J. Karam, G. Stormo* & L. Gold*

MeSH headings : Amino Acid Sequence; Base Sequence; Gene Expression Regulation; Genes, Viral; Nucleic Acid Conformation; Protein Biosynthesis; Repressor Proteins / genetics; T-Phages / genetics; Transcription Factors / genetics; Viral Proteins / genetics
TL;DR: The results suggest that the T4 regA gene product is a 122 amino acid polypeptide that is mildly basic and hydrophilic in character; these features are consistent with known properties of regA protein derived from T4-infected cells. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: January 19, 2020

1984 conference paper

Translational regulation during bacteriophage T4 development

In B. F. C. Clark & H. U. Petersen (Eds.), Gene expression : the translation step and its control : proceedings of the Alfred Benzon Symposium 19 held at the premises of the Royal Danish Academy of Sciences and Letters, Copenhagen, 19-23 June 1983. Copenhagen: Munskgaard.

By: L. Gold, M. Inman, E. Miller, D. Pribnow, T. Schneider, S. Shinedling, G. Stormo

Ed(s): B. Clark & H. Petersen

Event: Alfred Benzon Symposium 19 (Royal Danish Academy of Sciences and Letters) at Copenhagen on June 19-23, 1983

Source: NC State University Libraries
Added: January 29, 2021

1981 journal article

L-Methionine SR-sulfoximine resistant glutamine synthetase from mutants of Salmonella typhimurium

Journal of Biological Chemistry, 256(21), 11307–11312.

By: E. Miller* & J. Brenchley*

TL;DR: The data show that the glutamine synthetase protein has been altered by this mutation, designated as glnA982, and suggest that the L-methionine SR-sulfoximine resistance is conferred by a change in the NH3 binding domain of the enzyme. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: January 29, 2021

1978 journal article

Hematozoa of passeriform birds from Eagle Lake, California

Proceedings of the Helminthological Society of Washington, 45(2), 266–268.

By: E. Miller, R. Demaree & S. Tinling

Source: NC State University Libraries
Added: January 29, 2021

Employment

Updated: December 19th, 2019 16:09

2013 - present

North Carolina State University Raleigh, NC, US
Professor Plant & Microbial Biology

1986 - 2013

North Carolina State University Raleigh, NC, US
Assistant - Full Professor Microbiology

Education

Updated: July 1st, 2017 15:35

Purdue University West Lafayette, IN, US
PhD Biological Sciences

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