Eric S. Miller
Eric S. Miller, PhD Professor of Microbiology NC State University Raleigh, NC 27695
Works (56)
Updated: April 3rd, 2024 20:15
2023 review
Tolyporphins-Exotic Tetrapyrrole Pigments in a Cyanobacterium-A Review
[Review of ]. MOLECULES, 28(16).
author keywords: bacteriochlorin; bioinformatics; biosynthesis; chlorophyll; cyanobacteria; field biology; gene cluster; genomics; natural products; tolyporphins
TL;DR:
The identification of putative biosynthetic gene clusters in the HT-58-2 cyanobacterial genome and accompanying studies suggest a new biosynthesis paradigm in the tetrapyrrole arena, which stands alone in the large pigments-of-life family.
(via Semantic Scholar)
www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 2, 2023
2021 journal article
Identification of Putative Biosynthetic Gene Clusters for Tolyporphins in Multiple Filamentous Cyanobacteria
LIFE-BASEL, 11(8).
author keywords: tolyporphins; tetrapyrroles; cyanobacteria; biosynthetic gene cluster; Brasilonema; Nostoc; Oculatella
TL;DR:
Phylogenetic analysis based on 16S rRNA and a common component of the BGCs, TolD, points to a close evolutionary history between each strain and their respective tolyporphins BGC, and a collection of candidate cyanobacteria for genetic and biochemical analysis pertaining to these unusual tetrapyrrole macrocycles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2021
2021 journal article
Natural Product Gene Clusters in the Filamentous Nostocales Cyanobacterium HT-58-2
LIFE-BASEL, 11(4).
author keywords: anatoxin-a; homoanatoxin-a; hapalosin; heterocyst glycolipids; natural products; secondary metabolites; shinorine; tolypodiols; tolyporphins
TL;DR:
The results point to the rich biosynthetic capacity of the HT-58-2 cyanobacterium beyond the production of tolyporphins and tolypodiols, as well as the biosynthesis of other natural products.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 17, 2021
2019 journal article
Cellular localization of tolyporphins, unusual tetrapyrroles, in a microbial photosynthetic community determined using hyperspectral confocal fluorescence microscopy
Photosynthesis Research, 141(3), 259–271.
author keywords: Bacteriochlorin; Chlorophyll; Cyanobacteria; Membrane; Multivariate curve resolution
MeSH headings : Adaptation, Physiological; Bacteriochlorophyll A / chemistry; Chlorophyll A / chemistry; Cyanobacteria / metabolism; Cyanobacteria / ultrastructure; Darkness; Microscopy, Confocal; Microscopy, Fluorescence; Photosynthesis; Porphyrins / chemistry; Porphyrins / metabolism; Tetrapyrroles / chemistry; Tetrapyrroles / metabolism
TL;DR:
This work quantifies the relative abundance and envelope localization of tolyporphins in single cells, and illustrates the ability to identify novel tetrapyrroles in the presence of chlorophyll a in a photosynthetic microorganism within a non-axenic culture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: September 23, 2019
2018 journal article
Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium
Microbiology, 164(10), 1229–1239.
author keywords: alphaproteobacteria; fluorescence in situ hybridization (FISH); tolyporphins; bacteriochlorin; photosynthetic gene cluster
MeSH headings : Alphaproteobacteria / classification; Alphaproteobacteria / genetics; Alphaproteobacteria / metabolism; Alphaproteobacteria / physiology; Bacterial Proteins / genetics; Base Sequence; Chromosome Mapping; Cyanobacteria / metabolism; DNA, Bacterial / genetics; Genome, Bacterial / genetics; In Situ Hybridization, Fluorescence; Microbial Consortia; Phylogeny; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR:
The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT- 58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 7, 2019
2017 journal article
An inclusive Research Education Community (iREC): Impact of the SEA-PHAGES program on research outcomes and student learning
Proceedings of the National Academy of Sciences, 114(51), 13531–13536.
author keywords: bacteriophage; genomics; science education; evolution; assessment
MeSH headings : Biomedical Research / education; Biomedical Research / standards; Education, Medical, Undergraduate / methods; Education, Medical, Undergraduate / standards; Female; Humans; Learning; Male; Program Evaluation; Teaching; Universities / standards; Young Adult
TL;DR:
The SEA-PHAGES students show strong gains correlated with persistence relative to those in traditional laboratory courses regardless of academic, ethnic, gender, and socioeconomic profiles.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
2017 journal article
Genome Sequence and Composition of a Tolyporphin-Producing Cyanobacterium-Microbial Community
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(19).
author keywords: cyanobacteria; genome analysis; phylogenetic analysis; tetrapyrroles; tolyporphin
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosynthetic Pathways; Cyanobacteria / chemistry; Cyanobacteria / genetics; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Genome, Bacterial; Metagenomics; Multigene Family; Phylogeny; Porphyrins / biosynthesis; Porphyrins / chemistry
TL;DR:
It is found that the HT-58-2 culture is composed of the cyanobacterium and a community of associated bacteria, complicating the question of which organisms make tolyporphins, and an intriguing gene cluster that contains tetrapyrrole biosynthesis genes and a collection of unknown genes suggests a possible role in toly porphin biosynthesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Mass spectrometric detection of chlorophyll a and the tetrapyrrole secondary metabolite tolyporphin A in the filamentous cyanobacterium HT-58-2. Approaches to high-throughput screening of intact cyanobacteria
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, 21(11), 759–768.
author keywords: IR-MALDESI-FTMS; MS/MS; MALDI-TOF-MS; chlorophyll; bacteriochlorin; high-throughput screening
TL;DR:
Examination by MALDI-TOF-MS of lipophilic crude extracts of small-scale HT-58-2 samples without chromatographic fractionation enabled semi-quantitation of tolyporphin A over a 41-day growth period.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Photophysical Characterization of the Naturally Occurring Dioxobacteriochlorin Tolyporphin A and Synthetic Oxobacteriochlorin Analogues
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 93(5), 1204–1215.
MeSH headings : Cyanobacteria / chemistry; Photochemistry; Porphyrins / chemistry; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet
TL;DR:
The photophysical characterization is reported of tolyporphin A and two synthetic analogues, an oxobacteriochlorin and a dioxobacterium‐containing culture known as HT‐58‐2, which generally exhibits spectral and photophysical features resembling those of chlorophyll a.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Quantitation of Tolyporphins, Diverse Tetrapyrrole Secondary Metabolites with Chlorophyll-Like Absorption, from a Filamentous Cyanobacterium-Microbial Community
Phytochemical Analysis, 29(2), 205–216.
author keywords: HPLC; mass spectrometry; absorption spectroscopy with multicomponent analysis; nitrogen deprivation; growth curve
MeSH headings : Chlorophyll / chemistry; Chlorophyll A; Chromatography, High Pressure Liquid / methods; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Porphyrins / analysis; Porphyrins / chemistry; Reference Standards; Reproducibility of Results; Spectrum Analysis / methods; Tetrapyrroles / analysis
TL;DR:
The quantitative methods developed herein should facilitate investigation of the biosynthesis of tolyporphins (and other tetrapyrroles) as well as examination of other strains for production of tolporph Ins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2017 journal article
Vibrio Phage KVP40 Encodes a Functional NAD(+) Salvage Pathway
JOURNAL OF BACTERIOLOGY, 199(9).
author keywords: NAD; bacteriophages; genomics; metabolism
MeSH headings : Bacteriophage T4 / genetics; Escherichia coli / genetics; Genomics; Metabolic Networks and Pathways / genetics; NAD / metabolism; Nicotinamide-Nucleotide Adenylyltransferase / genetics; Nicotinamide-Nucleotide Adenylyltransferase / metabolism; Nucleotides; Pyridines / chemistry; Pyrophosphatases / metabolism; Vibrio parahaemolyticus / virology
TL;DR:
It is shown that phage KVP40 encodes a functional pyridine nucleotide scavenging pathway that is expressed during the metabolic period of the infection cycle, and the pathway is conserved in other large, dsDNA phages in which the two genes, nadV and natV, share an evolutionary history in their respective phage-host group.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Genome Sequence of Aeromicrobium erythreum NRRL B-3381, an Erythromycin-Producing Bacterium of the Nocardioidaceae
Genome Announcements, 4(2).
TL;DR:
Aeromicrobium erythreum NRRL B-3381 has a 3,629,239-bp circular genome that has 72% G+C content and provides resources for metabolic and combinatorial engineering of polyketides.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
2016 journal article
Paenibacillus larvae Phage Tripp Genome Has 378-Base-Pair Terminal Repeats
Genome Announcements, 4(1).
TL;DR:
Paenibacillus larvae bacteriophage Tripp was isolated from an American foulbrood diseased honey bee hive in North Carolina, USA and is currently unique in Genbank.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: January 19, 2020
2015 report
Divavirus taxonomic bacteriophage group infecting Paenibacillus larvae
International Committee on the Taxonomy of Viruses.
Source: NC State University Libraries
Added: February 6, 2021
2015 journal article
Genome Sequences of Six Paenibacillus larvae Siphoviridae Phages
Genome Announcements, 3(3).
TL;DR:
Six sequenced and annotated genomes of Paenibacillus larvae phages isolated from the combs of American foulbrood-diseased beehives show Phage Lily is most divergent from Diva, Rani, Redbud, Shelly, and Sitara.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
2015 journal article
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
ELife, 4.
MeSH headings : Biomedical Research / ethics; Cooperative Behavior; DNA, Viral / genetics; Gene Flow; Genetic Variation; Genome, Viral; High-Throughput Nucleotide Sequencing; Information Dissemination; Mosaicism; Mycobacteriophages / classification; Mycobacteriophages / genetics; Mycobacterium smegmatis / virology; Phylogeny; Phylogeography; Workforce
TL;DR:
To understand the population structure of phages infecting a single host strain, 627 phages of Mycobacterium smegmatis were isolated, sequenced, and compared to reveal a continuum of genetic diversity, albeit with uneven representation of different phages.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
2014 conference paper
Modeling ribosome dynamics to optimize heterologous protein production in escherichia coli
2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP). Presented at the 2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP).
Event: 2014 IEEE Global Conference on Signal and Information Processing (GlobalSIP)
TL;DR:
A free-energy based model of translation elongation to predict and optimize genes for expression in E. coli is presented and protein yield of optimized genes showed increase from their wildtype levels.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 7, 2020
2013 journal article
Introducing primary scientific literature to first-year undergraduate researchers
Council on Undergraduate Research on the Web, 34(4), 17–22.
Source: NC State University Libraries
Added: February 6, 2021
2010 article
Bacteriophage T4 and its relatives
Karam, J. D., & Miller, E. S. (2010, October 28). VIROLOGY JOURNAL, Vol. 7.
TL;DR:
The review series to be published in Virology Journal will emphasize advances and seminal discoveries in four major areas of T4 research: Genomics, Gene Expression, DNA Replication and Phage Morphogenesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 review
Genomes of the T4-related bacteriophages as windows on microbial genome evolution
[Review of ]. VIROLOGY JOURNAL, 7.
MeSH headings : Bacteriophages / genetics; Biological Evolution; DNA, Viral / chemistry; DNA, Viral / genetics; Evolution, Molecular; Genetic Variation; Genome, Viral; Molecular Sequence Data; Sequence Analysis, DNA
TL;DR:
The high degree of genetic heterogeneity that exists outside of this shared core suggests that horizontal DNA transfer involving many genetic sources has played a major role in diversification of the T4-related phages and their spread to a wide spectrum of bacterial species domains in evolution.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 review
Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation
[Review of ]. VIROLOGY JOURNAL, 7.
MeSH headings : Bacteriophage T4 / genetics; Gene Expression Regulation; Protein Biosynthesis; RNA Processing, Post-Transcriptional; RNA Stability / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism
TL;DR:
The past 10 - 15 year literature on RNA-protein interactions with T4 and some of its related phages is compiled, with particular focus on advances in mRNA decay and processing, and on translational repression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 journal article
Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases
TRANSGENIC RESEARCH, 17(1), 47–57.
author keywords: fungal resistance; Magnaporthe grisea; Rhizoctonia solani; T4 lysozyme; tall fescue
MeSH headings : Bacteriophage T4 / enzymology; Bacteriophage T4 / genetics; Cloning, Molecular; Festuca / enzymology; Festuca / genetics; Festuca / microbiology; Gene Expression; Genes, Viral; Genetic Vectors; Magnaporthe / pathogenicity; Muramidase / genetics; Plant Diseases / genetics; Plant Diseases / microbiology; Plants, Genetically Modified; Rhizobium / genetics; Rhizoctonia / pathogenicity
TL;DR:
It is demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants, which may have wide applications in engineered fungal disease resistance in various crops.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
In vitro selection of phage RB69 RegA RNA binding sites yields UAA repeats
VIROLOGY, 336(1), 26–36.
author keywords: RNA SELEX; translation repressor; T4-type phage
MeSH headings : Binding Sites / genetics; Codon, Terminator / genetics; Directed Molecular Evolution; Electrophoretic Mobility Shift Assay; Genes, Viral / genetics; Protein Binding; RNA, Viral / genetics; RNA, Viral / metabolism; RNA-Binding Proteins / metabolism; Repetitive Sequences, Nucleic Acid / genetics; Repressor Proteins / metabolism; Viral Proteins / metabolism
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
A family of anti-sigma(70) proteins in T4-type phages and bacteria that are similar to AsiA, a transcription inhibitor and co-activator of bacteriophage T4
JOURNAL OF MOLECULAR BIOLOGY, 344(5), 1183–1197.
author keywords: phage; AsiA; Rsd; AlgQ; transcription
MeSH headings : Amino Acid Sequence; Bacteriophage T4 / chemistry; Bacteriophage T4 / genetics; Cell Proliferation; DNA-Binding Proteins / metabolism; DNA-Directed RNA Polymerases / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Gene Expression Regulation; Holoenzymes / chemistry; Holoenzymes / metabolism; Molecular Sequence Data; Multigene Family; Multiprotein Complexes; Phenylalanine / genetics; Phenylalanine / metabolism; Protein Binding; Repressor Proteins / chemistry; Repressor Proteins / metabolism; Sequence Alignment; Sigma Factor / metabolism; Transcription Factors / metabolism; Transcription, Genetic; Two-Hybrid System Techniques; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
It is speculated that the phage AsiA orthologs, Rsd, and AlgQ are members of a related family in T4-type phage and bacteria, which interact similarly with primary sigma factors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
Characterization of bacteriophage KVP40 and T4 RNA ligase 2
VIROLOGY, 319(1), 141–151.
author keywords: bacteriophage; KVP40; T4 RNA ligase 2
MeSH headings : Adenosine Monophosphate / metabolism; Adenosine Triphosphate / metabolism; Amino Acid Sequence; Bacteriophages / enzymology; Carbohydrates / analysis; Molecular Sequence Data; RNA / chemistry; RNA / metabolism; RNA Caps / chemistry; RNA Caps / metabolism; RNA Ligase (ATP) / classification; RNA Ligase (ATP) / genetics; RNA Ligase (ATP) / metabolism; Recombinant Proteins / metabolism; Sequence Alignment; Viral Proteins / classification; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The ATP-dependent "capping" of RNA with AMP by Rnl2 is reminiscent of the capping of eukaryotic mRNA with GMP by GTP:RNA guanylyltransferase and suggests an evolutionary connection between bacteriophage Rnl 2 and eukARYotic RNA capping enzymes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 review
Bacteriophage T4 genome
[Review of ]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 67(1), 86-+.
MeSH headings : Bacteriophage T4 / genetics; Bacteriophage T4 / pathogenicity; DNA Repair; DNA Replication; DNA, Viral / metabolism; Genome, Viral; Lysogeny; Models, Molecular; Oligonucleotide Array Sequence Analysis; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Viral / chemistry; Ribosomes / metabolism; Transcription, Genetic
TL;DR:
T4 functional genomics will aid in the interpretation of these newly sequenced T4-related genomes and in broadening the understanding of the complex evolution and ecology of phages—the most abundant and among the most ancient biological entities on Earth.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2003 journal article
Complete genome sequence of the broad-host-range vibriophage KVP40: Comparative genomics of a T4-related bacteriophage
JOURNAL OF BACTERIOLOGY, 185(17), 5220–5233.
MeSH headings : Bacteriophage T4 / genetics; Genome, Viral; Genomics; Molecular Sequence Data; Myoviridae / genetics; Myoviridae / physiology; Sequence Analysis, DNA; Vibrio parahaemolyticus / virology; Viral Proteins / genetics
TL;DR:
Features of the KVP40 genome that distinguish it from T4 are presented, as well as those, such as the replication and virion gene clusters, that are substantially conserved.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2000 journal article
Subtilisins of Bacillus spp. hydrolyze keratin and allow growth on feathers
CANADIAN JOURNAL OF MICROBIOLOGY, 46(11), 1004–1011.
author keywords: keratin hydrolysis; Bacillus; subtilisin; keratinase
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
RNA-binding properties of in vitro expressed histidine-tagged RB69 RegA translational repressor protein
ANALYTICAL BIOCHEMISTRY, 269(1), 32–37.
MeSH headings : Bacteriophage T4 / genetics; Chromatography, Affinity / methods; Escherichia coli / metabolism; Histidine / genetics; Nickel / metabolism; Polymerase Chain Reaction / methods; Protein Biosynthesis; RNA / metabolism; RNA-Binding Proteins / biosynthesis; RNA-Binding Proteins / genetics; RNA-Binding Proteins / metabolism; Recombinant Proteins / metabolism; Repressor Proteins / biosynthesis; Repressor Proteins / genetics; Repressor Proteins / metabolism; Templates, Genetic; Viral Proteins / biosynthesis; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
In vitro synthesis of His-tagged proteins directly from linear PCR products provides a convenient and efficient system to study RegA and other interesting RNA-binding proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 patent
DNA encoding Bacillus lichenformis PWD-1 keratinase
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
An E-coli B mutation, rpoB5081, that prevents growth of phage T4 strains defective in host DNA degradation
FEMS Microbiology Letters, 157(1), 109–116.
MeSH headings : Alleles; Bacteriophage T4 / growth & development; Chromosome Mapping; DNA Repair; DNA, Bacterial / biosynthesis; DNA, Bacterial / metabolism; DNA-Directed RNA Polymerases / chemistry; DNA-Directed RNA Polymerases / metabolism; Endonucleases / metabolism; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / virology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Mutation; Phenotype; Sequence Analysis, DNA; Transcription Factors / metabolism; Transcription, Genetic; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
An E. coli B Tab strain was isolated that restricts T4 denA (DNA endonuclease II) mutants at 37 degrees C and above, but is permissive for wild-type T4 at all temperatures examined, and is an important host for screening and mapping T 4 denA mutations.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
1997 article
Expression of the Bacillus licheniformis PWD-1 keratinase gene in B-subtilis
Lin, X., Wong, S. L., Miller, E. S., & Shih, J. C. H. (1997, August). JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, Vol. 19, pp. 134–138.
author keywords: keratinase; gene cloning; gene expression; Bacilli
MeSH headings : Bacillus / enzymology; Bacillus subtilis / genetics; Cloning, Molecular; Glucose / pharmacology; Peptide Hydrolases / biosynthesis; Peptide Hydrolases / genetics; Plasmids; Recombinant Proteins / biosynthesis
TL;DR:
The kerA gene which encodes the enzyme keratinase was isolated from the feather-degrading bacterium Bacillus licheniformis PWD-1 and transformants and was further characterized when glucose or casamino acids were supplemented into the feather medium.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1995 journal article
Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1.
Applied and Environmental Microbiology, 61(4), 1469–1474.
MeSH headings : Amino Acid Sequence; Animals; Bacillus / enzymology; Bacillus / genetics; Bacteriophage lambda / genetics; Base Sequence; Biodegradation, Environmental; DNA Primers / genetics; DNA, Bacterial / genetics; Feathers; Gene Expression Regulation, Bacterial; Gene Library; Genes, Bacterial; Molecular Sequence Data; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Phenylmethylsulfonyl Fluoride / pharmacology; Polymerase Chain Reaction / methods; Poultry; Sequence Homology, Amino Acid; Subtilisins / genetics
TL;DR:
NorthernRNA analysis demonstrates that transcriptional regulation controls kerA expression on different growth media and deduced amino acid sequences indicate only three amino acid differences between the two mature proteases.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1995 journal article
RNA-protein interactions of the bacteriophage RB69 RegA translational repressor protein
Nucleic Acids Symposium Series, 33, 256–257.
Source: NC State University Libraries
Added: January 23, 2021
1994 chapter
Control of translation initiation: mRNA structure and protein repressors
In J. D. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular Biology of Bacteriophage T4 (pp. 193–205). Washington, D.C: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
Detection of plasmid encoded T4 proteins: radiolabeling and biological activity
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
Experiments in T4 genetics
In J. D. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular Biology of Bacteriophage T4 (pp. 421–483). Washington, D.C: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
General considerations for strain construction
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4 (pp. 438–441). Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
General procedures for manipulating bacteriophage T4
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
Large scale isolation of mRNA from T4-infected cells
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 book
Molecular Biology of Bacteriophage T4
Washington, D.C: ASM Press.
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
PCR amplification of DNA from T4 plaques
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
Quantitation of T4 DNA synthesis by radiolabelling
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
Radiolabelling of T4 proteins and their analysis by gel electrophoresis
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hall, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4. Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hall, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1994 chapter
Rapid screening of phage progeny from multiple-factor crosses
In J. Karam, J. W. Drake, K. N. Kreuzer, G. Mosig, D. H. Hill, F. A. Eiserling, … E. S. Miller (Eds.), Molecular biology of bacteriophage T4 (pp. 442–443). Washington, D.C.: ASM Press.
Ed(s): J. Karam, J. Drake, K. Kreuzer, G. Mosig, D. Hill, F. Eiserling, L. Black, E. Spicer
Source: NC State University Libraries
Added: January 28, 2021
1992 journal article
Regions of bacteriophage T4 and RB69 RegA translational repressor proteins that determine RNA-binding specificity.
Proceedings of the National Academy of Sciences, 89(11), 5053–5057.
MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; Gene Expression Regulation, Viral; Genes, Viral; Molecular Sequence Data; Mutation; Protein Biosynthesis; RNA, Messenger / metabolism; RNA-Binding Proteins / chemistry; RNA-Binding Proteins / metabolism; Repressor Proteins / chemistry; Sequence Alignment; Structure-Activity Relationship; T-Phages / genetics; Viral Proteins / chemistry; Viral Structural Proteins / genetics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1991 journal article
Cloning vectors, mutagenesis, and gene disruption (ermR) for the erythromycin-producing bacterium Aeromicrobium erythreum.
Applied and Environmental Microbiology, 57(9), 2758–2761.
MeSH headings : Amino Acids / physiology; Chromosomes, Bacterial; Cloning, Molecular; DNA Replication; Erythromycin / biosynthesis; Genes, Bacterial; Genetic Vectors; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Mutagenesis; Plasmids; Recombination, Genetic
TL;DR:
P Phenotypic characterizations demonstrated that ermR is the sole determinant of macrolide antibiotic resistance in A. erythreum.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1991 journal article
Description of the Erythromycin-Producing Bacterium Arthrobacter sp. Strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov.
International Journal of Systematic Bacteriology, 41(3), 363–368.
MeSH headings : Amino Acids / analysis; Arthrobacter / classification; Arthrobacter / metabolism; Bacteria, Aerobic / classification; Bacteria, Aerobic / metabolism; Base Composition; Base Sequence; Erythromycin / metabolism; Fatty Acids / analysis; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / metabolism; Microscopy, Electron; Molecular Sequence Data; Peptidoglycan / chemistry; Phenotype; RNA, Ribosomal, 16S / genetics; Sequence Homology, Nucleic Acid; Soil Microbiology; Vitamin K / analysis
TL;DR:
The principal long-chain fatty acids which were identified that distinguished strain NRRL B-3381T from related G + C-rich bacteria were 10-methyloctadecanoic (tuberculosteric), octadecenoic, and hexadecanoIC acids, which form the basis for the recommendation that strain NR RL B- 3381 should be the type strain of a new taxon, for which the name Aeromicrobium erythreum is proposed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1990 journal article
Sequence analysis of conserved regA and variable orf43.1 genes in T4-like bacteriophages.
Journal of Bacteriology, 172(9), 5180–5186.
MeSH headings : Amino Acid Sequence; Base Sequence; Coliphages / genetics; DNA Transposable Elements; Escherichia coli / genetics; Genes, Viral; Molecular Sequence Data; Oligonucleotide Probes; T-Phages / genetics; Viral Proteins / genetics
TL;DR:
Bacteriophage T4 RegA protein is a translational repressor of several phage mRNAs in the T4-related phages examined, regA nucleotide sequences are highly conserved and the inferred amino acid sequences are identical.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1987 journal article
Translational repression: Biological activity of plasmid-encoded bacteriophage T4 RegA protein
Journal of Molecular Biology, 194(3), 397–410.
MeSH headings : Base Sequence; Binding Sites; DNA, Viral / genetics; Gene Expression Regulation; Plasmids; Protein Biosynthesis; Repressor Proteins / genetics; T-Phages / genetics; Transcription, Genetic; Viral Proteins / genetics
TL;DR:
Cloned wild-type and mutant alleles of the T4 regA gene under control of the heat-inducible, plasmid-borne leftward promoter of phage lambda resulted in the synthesis of a protein that closely resembled phage-encoded RegA protein in biological properties.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1985 journal article
Bacteriophage T4 RegA protein. Purification of a translational repressor
Journal of Biological Chemistry, 260(24), 13053–13059.
TL;DR:
The bacteriophage T4 regA protein translationally regulates its own synthesis and the synthesis of several other T4 early proteins and the mechanism of translational regulation is studied in relation to the mechanism by which the protein recognizes its mRNA targets.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: January 29, 2021
1984 journal article
Cloning and characterization of gdhA, the structural gene for glutamate dehydrogenase of Salmonella typhimurium.
Journal of Bacteriology, 157(1), 171–178.
MeSH headings : Cloning, Molecular; DNA, Bacterial / genetics; Escherichia coli / enzymology; Escherichia coli / genetics; Genes; Genes, Bacterial; Genetic Complementation Test; Glutamate Dehydrogenase / genetics; Glutamate Synthase / genetics; Mutation; Operon; Plasmids; Salmonella typhimurium / enzymology; Salmonella typhimurium / genetics
TL;DR:
S. typhimurium gdhA DNA was shown to hybridize to single restriction fragments of chromosomes from other enteric bacteria and from Saccharomyces cerevisiae, and was regulated in both organisms.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1984 journal article
The bacteriophage T4regAgene: primary sequence of a translational repressor
Nucleic Acids Research, 12(15), 5979–5993.
MeSH headings : Amino Acid Sequence; Base Sequence; Gene Expression Regulation; Genes, Viral; Nucleic Acid Conformation; Protein Biosynthesis; Repressor Proteins / genetics; T-Phages / genetics; Transcription Factors / genetics; Viral Proteins / genetics
TL;DR:
The results suggest that the T4 regA gene product is a 122 amino acid polypeptide that is mildly basic and hydrophilic in character; these features are consistent with known properties of regA protein derived from T4-infected cells.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 19, 2020
1984 conference paper
Translational regulation during bacteriophage T4 development
In B. F. C. Clark & H. U. Petersen (Eds.), Gene expression : the translation step and its control : proceedings of the Alfred Benzon Symposium 19 held at the premises of the Royal Danish Academy of Sciences and Letters, Copenhagen, 19-23 June 1983. Copenhagen: Munskgaard.
Ed(s): B. Clark & H. Petersen
Event: Alfred Benzon Symposium 19 (Royal Danish Academy of Sciences and Letters) at Copenhagen on June 19-23, 1983
Source: NC State University Libraries
Added: January 29, 2021
1981 journal article
L-Methionine SR-sulfoximine resistant glutamine synthetase from mutants of Salmonella typhimurium
Journal of Biological Chemistry, 256(21), 11307–11312.
TL;DR:
The data show that the glutamine synthetase protein has been altered by this mutation, designated as glnA982, and suggest that the L-methionine SR-sulfoximine resistance is conferred by a change in the NH3 binding domain of the enzyme.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: January 29, 2021
1978 journal article
Hematozoa of passeriform birds from Eagle Lake, California
Proceedings of the Helminthological Society of Washington, 45(2), 266–268.
Source: NC State University Libraries
Added: January 29, 2021
Employment
Updated: December 19th, 2019 16:09
2013 - present
North Carolina State University Raleigh, NC, US
Professor
Plant & Microbial Biology
1986 - 2013
North Carolina State University Raleigh, NC, US
Assistant - Full Professor
Microbiology
Education
Updated: July 1st, 2017 15:35
Purdue University West Lafayette, IN, US
PhD
Biological Sciences
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