@article{adedipe_grubbs_coates_wiegmman_lorenzen_2019, title={Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family}, volume={20}, ISSN={1471-2164}, url={http://dx.doi.org/10.1186/s12864-019-6218-8}, DOI={10.1186/s12864-019-6218-8}, abstractNote={AbstractBackgroundThe western corn rootworm,Diabrotica virgifera virgifera, is a pervasive pest of maize in North America and Europe, which has adapted to current pest management strategies. In advance of an assembled and annotatedD. v. virgiferagenome, we developed transcriptomic resources to use in identifying candidate genes likely to be involved in the evolution of resistance, starting with members of the ATP-binding cassette (ABC) transporter family.ResultsIn this study, 65 putativeD. v. virgiferaABC (DvvABC) transporters were identified within a combined transcriptome assembly generated from embryonic, larval, adult male, and adult female RNA-sequence libraries. Phylogenetic analysis placed the deduced amino-acid sequences of theDvvABC transporters into eight subfamilies (A to H). To supplement our sequence data with functional analysis, we identified orthologs ofTribolium castaneumABC genes which had previously been shown to exhibit overt RNA interference (RNAi) phenotypes. We identified eight suchD. v. virgiferagenes, and found that they were functionally similar to theirT. castaneumcounterparts. Interestingly, depletion ofDvvABCB_39715andDvvABCG_3712transcripts in adult females produced detrimental reproductive and developmental phenotypes, demonstrating the potential of these genes as targets for RNAi-mediated insect control tactics.ConclusionsBy combining sequence data from four libraries covering three distinct life stages, we have produced a relatively comprehensive de novo transcriptome assembly forD. v. virgifera. Moreover, we have identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species.}, number={1}, journal={BMC Genomics}, publisher={Springer Science and Business Media LLC}, author={Adedipe, Folukemi and Grubbs, Nathaniel and Coates, Brad and Wiegmman, Brian and Lorenzen, Marcé}, year={2019}, month={Nov} }
 @article{oppert_guedes_aikins_perkin_chen_phillips_zhu_opit_hoon_sun_et al._2015, title={Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum}, volume={16}, ISSN={["1471-2164"]}, DOI={10.1186/s12864-015-2121-0}, abstractNote={Phosphine is a valuable fumigant to control pest populations in stored grains and grain products. However, recent studies indicate a substantial increase in phosphine resistance in stored product pests worldwide.To understand the molecular bases of phosphine resistance in insects, we used RNA-Seq to compare gene expression in phosphine-resistant and susceptible laboratory populations of the red flour beetle, Tribolium castaneum. Each population was evaluated as either phosphine-exposed or no phosphine (untreated controls) in triplicate biological replicates (12 samples total). Pairwise analysis indicated there were eight genes differentially expressed between susceptible and resistant insects not exposed to phosphine (i.e., basal expression) or those exposed to phopshine (>8-fold expression and 90 % C.I.). However, 214 genes were differentially expressed among all four treatment groups at a statistically significant level (ANOVA, p < 0.05). Increased expression of 44 cytochrome P450 genes was found in resistant vs. susceptible insects, and phosphine exposure resulted in additional increases of 21 of these genes, five of which were significant among all treatment groups (p < 0.05). Expression of two genes encoding anti-diruetic peptide was 2- to 8-fold reduced in phosphine-resistant insects, and when exposed to phosphine, expression was further reduced 36- to 500-fold compared to susceptible. Phosphine-resistant insects also displayed differential expression of cuticle, carbohydrate, protease, transporter, and many mitochondrial genes, among others. Gene ontology terms associated with mitochondrial functions (oxidation biological processes, monooxygenase and catalytic molecular functions, and iron, heme, and tetrapyyrole binding) were enriched in the significantly differentially expressed dataset. Sequence polymorphism was found in transcripts encoding a known phosphine resistance gene, dihydrolipoamide dehydrogenase, in both susceptible and resistant insects. Phosphine-resistant adults also were resistant to knockdown by the pyrethroid deltamethrin, likely due to the increased cytochrome P450 expression.Overall, genes associated with the mitochondria were differentially expressed in resistant insects, and these differences may contribute to a reduction in overall metabolism and energy production and/or compensation in resistant insects. These data provide the first gene expression data on the response of phosphine-resistant and -susceptible insects to phosphine exposure, and demonstrate that RNA-Seq is a valuable tool to examine differences in insects that respond differentially to environmental stimuli.}, journal={BMC GENOMICS}, author={Oppert, Brenda and Guedes, Raul N. C. and Aikins, Michael J. and Perkin, Lindsey and Chen, Zhaorigetu and Phillips, Thomas W. and Zhu, Kun Yan and Opit, George P. and Hoon, Kelly and Sun, Yongming and et al.}, year={2015}, month={Nov} }