Fang Li

Works (10)

Updated: April 24th, 2025 04:32

2021 journal article

A conditional female lethal system for genetic suppression of the global fruit crop pest Drosophila suzukii

Pest Management Science, 77(11), 4915–4922.

By: F. Li n, A. Yamamoto n, E. Belikoff n, A. Berger n, E. Griffith n  & M. Scott n 

author keywords: Drosophila suzukii; spotted wing Drosophila; sterile insect technique; fsRIDL; tet-OFF; alternate splicing
MeSH headings : Animals; Drosophila / genetics; Fruit; Housing, Animal; Rubus; Suppression, Genetic
topics (OpenAlex): Insect behavior and control techniques; Insect symbiosis and bacterial influences; Insect-Plant Interactions and Control
TL;DR: It is shown that repeated releases of an excess of transgenic males can suppress D. suzukii populations in laboratory cage trials and suggests that the transgenic strain could provide an effective approach for control of this invasive pest of soft-skinned fruits. (via Semantic Scholar)
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13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: July 19, 2021

2019 journal article

Specific Gene Disruption in the Major Livestock PestsCochliomyia hominivoraxandLucilia cuprinaUsing CRISPR/Cas9

G3 Genes|Genomes|Genetics, 9(9), 3045–3055.

By: D. Paulo *, M. Williamson n, A. Arp *, F. Li n, A. Sagel, S. Skoda, J. Sanchez-Gallego, M. Vasquez ...

author keywords: Functional genomics; reverse genetics; myiasis; New World Screwworm fly; Australian Sheep Blowfly; gene drive; CRISPR; Cas9; brown body; yellow; transformer
MeSH headings : Animals; Animals, Genetically Modified; CRISPR-Cas Systems; Diptera / embryology; Diptera / genetics; Embryo, Nonmammalian; Female; Insect Proteins / genetics; Male; Mutation; Pest Control / methods; RNA, Guide, Kinetoplastida; Sex Determination Processes
topics (OpenAlex): CRISPR and Genetic Engineering; Insect symbiosis and bacterial influences; Insect behavior and control techniques
TL;DR: The CRISPR protocol described here is a significant improvement on the existing toolkit of molecular methods in calliphorids and suggests that Cas9-based systems targeting Chtra and Lctra could be an effective means for controlling natural populations of these important pests. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: September 23, 2019

2016 journal article

A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique

BMC Biology, 14.

By: C. Concha, A. Palavesam, F. Guerrero, A. Sagel, F. Li, J. Osborne, Y. Hernandez, T. Pardo ...

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Towards next generation maggot debridement therapy: transgenic Lucilia sericata larvae that produce and secrete a human growth factor

BMC Biotechnology, 16(1).

By: R. Linger n, E. Belikoff n, Y. Yan n, F. Li n, H. Wantuch  n, H. Fitzsimons *, M. Scott n 

author keywords: Maggot debridement therapy (MDT); Platelet-derived growth factor (PDGF); Excretions/secretions (ES); Growth factor treatment; Diabetic foot ulcer; Tetracycline transactivator; Lucilia sericata; Wound healing
MeSH headings : Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / metabolism; Debridement / methods; Diabetic Foot; Diptera / genetics; Diptera / metabolism; Female; Gene Expression / drug effects; Humans; Larva; Male; Platelet-Derived Growth Factor / genetics; Platelet-Derived Growth Factor / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Tetracycline / pharmacology; Wound Healing
topics (OpenAlex): Forensic Entomology and Diptera Studies; Insect Utilization and Effects; Insect and Pesticide Research
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3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2015 journal article

CRISPR/Cas9-mediated mutagenesis of the white and Sex lethal loci in the invasive pest, Drosophila suzukii

Biochemical and Biophysical Research Communications, 469(4), 911–916.

By: F. Li n & M. Scott n 

author keywords: Drosophila suzukii; CRISPR; Cas9; white; Sex-lethal
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Drosophila / classification; Drosophila / genetics; Drosophila Proteins / genetics; Eye Color / genetics; Eye Proteins / genetics; Female; Male; Mutagenesis, Site-Directed / methods; Quantitative Trait Loci / genetics; RNA-Binding Proteins / genetics; Sex Characteristics
topics (OpenAlex): Insect behavior and control techniques; CRISPR and Genetic Engineering; Insect symbiosis and bacterial influences
TL;DR: The D. suzukii Sxl gene could be an excellent target for a Cas9-mediated gene drive to suppress populations of this highly destructive pest. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2014 journal article

Functional characterization of calliphorid cell death genes and cellularization gene promoters for controlling gene expression and cell viability in early embryos

Insect Molecular Biology, 24(1), 58–70.

By: R. Edman n, R. Linger n, E. Belikoff n, F. Li n, S. Sze*, A. Tarone *, M. Scott n 

author keywords: genetic sexing; transgenic; cellularization gene; sterile insect technique
MeSH headings : Amino Acid Sequence; Animals; Animals, Genetically Modified; Base Sequence; Cell Death / genetics; Cell Survival; Diptera / embryology; Diptera / genetics; Diptera / growth & development; Drosophila melanogaster / genetics; Drosophila melanogaster / growth & development; Embryo, Nonmammalian; Female; Gene Expression Regulation; Genes, Insect; Genes, Lethal; Male; Molecular Sequence Data; Pest Control, Biological / methods; Promoter Regions, Genetic; Sex Ratio
topics (OpenAlex): Insect Resistance and Genetics; Viral Infectious Diseases and Gene Expression in Insects; Insect behavior and control techniques
TL;DR: The isolated gene promoters and pro‐apoptotic genes could potentially be used to build transgenic embryonic sexing strains of calliphorid livestock pests. (via Semantic Scholar)
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Sources: Web Of Science, Crossref
Added: August 6, 2018

2014 journal article

Transgenic sexing system for genetic control of the Australian sheep blow fly Lucilia cuprina

Insect Biochemistry and Molecular Biology, 51, 80–88.

By: F. Li n, H. Wantuch  n, R. Linger n, E. Belikoff n & M. Scott n 

author keywords: Sterile insect technique; Transgenic; Lucilia cuprina; Flystrike; Genetic sexing
MeSH headings : Animals; Animals, Genetically Modified; Diptera / genetics; Female; Genes, Insect; Genes, Lethal; Male; Pest Control, Biological / methods; Sex Determination Processes
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Insect behavior and control techniques; Forensic Entomology and Diptera Studies
TL;DR: Transgenic strains of L. cuprina that carry a dominant tetracycline repressible female lethal genetic system are developed and should be readily transferrable to other major calliphorid livestock pests including the New and Old World screwworm. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2013 journal article

Conservation and Sex-Specific Splicing of the transformer Gene in the Calliphorids Cochliomyia hominivorax, Cochliomyia macellaria and Lucilia sericata

PLoS ONE, 8(2), e56303.

By: F. Li n, S. Vensko n, E. Belikoff n & M. Scott n 

Ed(s): G. Tear

MeSH headings : Animals; Base Sequence; Conserved Sequence / genetics; Diptera / genetics; Diptera / growth & development; Diptera / physiology; Female; Genes, Insect / genetics; Male; Molecular Sequence Data; Nucleotide Motifs / genetics; RNA Splicing / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism; Sex Characteristics
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Insect Resistance and Genetics; Insect and Pesticide Research
TL;DR: A model for sex specific regulation of tra splicing is proposed that incorporates the conserved features identified in this study and is proposed to facilitate the development of a “male-only” strain for genetic control programs. (via Semantic Scholar)
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Sources: Web Of Science, Crossref
Added: August 6, 2018

2012 journal article

A de novo transcriptome assembly of Lucilia sericata (Diptera: Calliphoridae) with predicted alternative splices, single nucleotide polymorphisms and transcript expression estimates

Insect Molecular Biology, 21(2), 205–221.

By: S. Sze, J. Dunham*, B. Carey*, P. Chang *, F. Li n, R. Edman n, C. Fjeldsted*, M. Scott n , S. Nuzhdin *, A. Tarone *

author keywords: Drosophila; Lucilia; transcriptome; de novo assembly; alternative splice; transposable element
MeSH headings : Alternative Splicing; Animals; Culicidae / genetics; DNA Transposable Elements; Diptera / genetics; Diptera / growth & development; Diptera / metabolism; Drosophila melanogaster / genetics; Female; Gene Expression; Genome, Insect; Male; Molecular Conformation; Multigene Family; Polymorphism, Single Nucleotide; Transcriptome
topics (OpenAlex): Forensic Entomology and Diptera Studies; Insect behavior and control techniques; Insect and Pesticide Research
TL;DR: The assembly of the transcriptome was carried out in a manner that enabled identification of putative single nucleotide polymorphisms (SNPs) and alternative splices, and that provided expression estimates for various life history stages and for salivary tissue. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2010 journal article

Sex-biased transcription enhancement by a 5' tethered Gal4-MOF histone acetyltransferase fusion protein in Drosophila

BMC Molecular Biology, 11(1).

By: A. Schiemann *, F. Li n, V. Weake *, E. Belikoff n, K. Klemmer*, S. Moore*, M. Scott n 

MeSH headings : Acetylation; Amino Acid Sequence; Animals; DNA-Binding Proteins / analysis; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Drosophila / enzymology; Drosophila / genetics; Drosophila / metabolism; Drosophila Proteins / analysis; Drosophila Proteins / genetics; Drosophila Proteins / metabolism; Female; Genes, Reporter; Genes, X-Linked; Histone Acetyltransferases / analysis; Histone Acetyltransferases / genetics; Histone Acetyltransferases / metabolism; Histones / genetics; Histones / metabolism; Male; Models, Molecular; Molecular Sequence Data; Nuclear Proteins / analysis; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Polytene Chromosomes / chemistry; Recombinant Fusion Proteins / analysis; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Sequence Alignment; Sex Factors; Transcription Factors / analysis; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation
topics (OpenAlex): Genomics and Chromatin Dynamics; RNA Research and Splicing; Epigenetics and DNA Methylation
TL;DR: Targeting MOF to reporter genes led to transcription enhancement and acetylation of histone H4 at lysine 16 and how association of Gal4-MOF with the MSL or NSL proteins could explain the results is discussed. (via Semantic Scholar)
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Sources: Web Of Science, Crossref
Added: August 6, 2018

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