Works (26)
Updated: August 3rd, 2023 21:17
2022 journal article
Evaluation of Protein Extraction Methods for Metaproteomic Analyses of Root-Associated Microbes
MOLECULAR PLANT-MICROBE INTERACTIONS, 35(11), 977–988.
author keywords: endosphere; metaproteomics; microbiome; microbiota; phytobiome; plant-microbe interactions; rhizoplane
MeSH headings : Arabidopsis; Microbiota; Chromatography, Liquid; Proteome; Mass Spectrometry; Plants
TL;DR:
This work optimized and evaluated different protein extraction methods for metaproteomics of plant-associated microbiota in two different plant species (Arabidopsis and maize) and found that the optimal extraction method differed between the two species.
(via Semantic Scholar)
doi.org (publisher)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: November 1, 2022
2021 journal article
Activation of the Type VI Secretion System in the Squid Symbiont Vibrio fischeri Requires the Transcriptional Regulator TasR and the Structural Proteins TssM and TssA
JOURNAL OF BACTERIOLOGY, 203(21).
author keywords: type VI secretion systems; Aliivibrio fischeri; microbial interactions; genomic island; symbiosis
MeSH headings : Aliivibrio fischeri / genetics; Aliivibrio fischeri / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Gene Expression Regulation, Bacterial / physiology; Genotype; Mutation; Promoter Regions, Genetic; Type VI Secretion Systems / genetics; Type VI Secretion Systems / metabolism
TL;DR:
A model whereby T6SS expression requires conserved structural proteins, in addition to the essential regulator TasR, and this ability to self-regulate may be a strategy to activate T 6SS expression upon transfer of T6 SS-encoding elements into a new bacterial host is proposed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 1, 2021
2021 review
Five key aspects of metaproteomics as a tool to understand functional interactions in host-associated microbiomes
(D. A. Hogan, Ed.) [Review of ]. PLOS PATHOGENS, 17(2).
Ed(s): D. Hogan
MeSH headings : Bacteria / classification; Bacteria / isolation & purification; Bacteria / metabolism; Computational Biology; Host Microbial Interactions; Humans; Microbiota; Proteome / analysis; Proteome / metabolism; Proteomics / methods
TL;DR:
The objective in this Pearl is to highlight what the authors feel are 5 essential elements to be considered for a metaproteomics research campaign and to introduce nonexpert readers to the topic without going into too much technical detail.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 26, 2021
2021 review
Mass spectrometry-based proteomic platforms for better understanding of SARS-CoV-2 induced pathogenesis and potential diagnostic approaches
[Review of ]. PROTEOMICS, 21(10).
author keywords: biomarkers; comparative proteomics; COVID‐ 19; kinase‐ substrate signaling; post‐ translational modifications; targeted proteomics; top‐ down proteomics
MeSH headings : Animals; COVID-19 / diagnosis; COVID-19 / metabolism; COVID-19 / pathology; Host-Pathogen Interactions; Humans; Mass Spectrometry / methods; Protein Interaction Mapping / methods; Protein Interaction Maps; Protein Kinases / analysis; Protein Kinases / metabolism; Protein Processing, Post-Translational; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Receptor, EphA2 / analysis; Receptor, EphA2 / metabolism; SARS-CoV-2 / physiology; Signal Transduction
TL;DR:
The results obtained by various mass spectrometry (MS)‐based proteomic approaches applied to the functional characterization of proteins and pathways associated with SARS‐CoV‐2‐mediated infections in humans are summarized and rationalized.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021
2021 journal article
Microbe-dependent heterosis in maize
Proceedings of the National Academy of Sciences, 118(30).
author keywords: hybrid vigor; microbiome; maize; endophytes; rhizosphere
MeSH headings : Bacteria / metabolism; Fungi / physiology; Hybrid Vigor; Seedlings / growth & development; Seedlings / microbiology; Soil Microbiology; Zea mays / growth & development; Zea mays / microbiology
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: July 21, 2021
2020 journal article
Environmental Viscosity Modulates Interbacterial Killing during Habitat Transition
MBIO, 11(1).
author keywords: Aliivibrio fischeri; aggregation; competition; proteomics; type VI secretion
MeSH headings : Aliivibrio fischeri / genetics; Aliivibrio fischeri / physiology; Animals; Decapodiformes / microbiology; Ecosystem; Gene Expression Regulation, Bacterial; Genotype; Proteomics; Seawater; Symbiosis; Type VI Secretion Systems / genetics; Type VI Secretion Systems / metabolism; Viscosity
TL;DR:
How environmental viscosity impacts T6SS-mediated competition by using a liquid hydrogel medium that mimics the viscous host environment is investigated and shows that competitor cells form aggregates under host-like conditions, thereby facilitating the contact required for killing, and reveals how V. fischeri regulates a key competitive mechanism in response to the physical environment.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 6, 2020
2020 journal article
Mass Spectrometry-Based Identification of Phospho-Tyr in Plant Proteomics
Journal of Proteome Research, 19(2), 561–571.
author keywords: mass spectrometry; plant proteomics; regulation; signaling; tyrosine phosphorylation
MeSH headings : Amino Acid Motifs; Chromatography, Affinity / methods; Gene Ontology; Mass Spectrometry / methods; Phosphoproteins / metabolism; Phosphorylation; Phosphotyrosine / metabolism; Plants / metabolism; Protein Processing, Post-Translational / genetics; Protein Processing, Post-Translational / physiology; Proteome / metabolism; Proteomics / methods; Signal Transduction / genetics; Signal Transduction / physiology; Tyrosine / analogs & derivatives; Tyrosine / metabolism; src Homology Domains
TL;DR:
A strategy is proposed that will allow a depth of coverage of the plant pY-proteome comparable to that achieved in mammalian systems and implies previously unappreciated roles for this "animal PTM" in plants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: January 23, 2020
2020 article
Microbe-dependent heterosis in maize
Wagner, M. R., Tang, C., Salvato, F., Clouse, K. M., Bartlett, A., Sermons, S., … Kleiner, M. (2020, May 7). (Vol. 5). Vol. 5.
TL;DR:
It is shown that heterosis of root biomass and other traits in maize is strongly dependent on the belowground microbial environment, an ecological phenomenon whereby soil microbes differentially impact the early growth of inbred and hybrid maize.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: ORCID
Added: May 8, 2020
2019 journal article
Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 171, 12–25.
author keywords: Cotyledon; Early seedling, Proteomics; Ricinus communis; Salinity
MeSH headings : Biomass; Cotyledon / metabolism; Energy Metabolism; Osmotic Pressure / physiology; Photosynthesis; Plant Leaves / metabolism; Plant Proteins / metabolism; Potassium / metabolism; Proline / metabolism; Proteomics; Ricinus; Salinity; Salt Tolerance; Seedlings / growth & development; Seedlings / metabolism; Sodium / metabolism; Sodium Chloride / analysis
TL;DR:
Physiological and proteomic results highlighted that cotyledons accumulated a large number of Na+ and provided more energy to help true leaves cope with salt stress, and the true leaves saved carbon structures to synthesize osmotic substances and could also maintain photosynthesis under salt stress.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 11, 2019
2018 journal article
Temporal dynamic responses of roots in contrasting tomato genotypes to cadmium tolerance
Ecotoxicology, 1.
author keywords: Antioxidants; Cadmium; Heavy metals; Oxidative stress; Solanum lycopersicum
MeSH headings : Antioxidants / metabolism; Cadmium / metabolism; Genotype; Solanum lycopersicum / enzymology; Solanum lycopersicum / genetics; Solanum lycopersicum / metabolism; Plant Roots / enzymology; Plant Roots / metabolism; Soil Pollutants / metabolism; Stress, Physiological; Time Factors
TL;DR:
Results suggest APX, GR and GST as the main players of the antioxidant machinery against Cd-induced oxidative stress against tomato genotypes contrasting in CD-tolerance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: June 21, 2019
2017 journal article
Comparative proteomic analysis provides insight into the biological role of protein phosphatase inhibitor-2 from Arabidopsis
Journal of Proteomics, 165, 51–60.
author keywords: Type one phosphatase; Phosphatase inhibitor; Comparative proteomics; Ribosome biogenesis; Protein-protein interaction
MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis Proteins / analysis; Arabidopsis Proteins / antagonists & inhibitors; Gene Expression Regulation, Plant; Gene Knockdown Techniques; Phosphoprotein Phosphatases / antagonists & inhibitors; Protein Interaction Maps; Proteins / genetics; Proteins / physiology; Proteomics / methods
TL;DR:
The complex, reduced growth phenotype supports the notion that AtPPI-2 is a global regulator of TOPPs, and possibly other proteins, and the proposed protein interaction network comprises a testable model underlying changes in protein abundance in the AtPPi-2 mutant, and provides a better framework for future studies.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2017 journal article
Luxurious Nitrogen Fertilization of Two Sugar Cane Genotypes Contrasting for Lignin Composition Causes Changes in the Stem Proteome Related to Carbon, Nitrogen, and Oxidant Metabolism but Does Not Alter Lignin Content
Journal of Proteome Research, 16(10), 3688–3703.
author keywords: Saccharum officinarum; cell wall; lignocellulosic ethanol; lignin; proteomics
MeSH headings : Biofuels; Biomass; Carbohydrates / chemistry; Carbohydrates / genetics; Fermentation; Gene Expression Regulation, Plant; Genotype; Lignin / chemistry; Lignin / metabolism; Nitrogen / chemistry; Nitrogen / metabolism; Oxidants / chemistry; Oxidants / metabolism; Phenotype; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Proteome / chemistry; Proteome / genetics; Saccharum / genetics; Saccharum / metabolism
TL;DR:
A large-scale proteomics approach based on GeLC-MS/MS was employed to identify and relatively quantify proteins differently accumulated in two contrasting genotypes for lignin composition after excessive nitrogen fertilization, finding that carbon metabolism, amino acid metabolism, protein turnover, and oxidative stress were affected.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2017 article
Proteomics for Bioenergy Production
Salvato, F., Santos, B. M., Santana Costa, M. G., & Rodriguez, E. A. G. (Eds.). (2017, June 11). Plant-Based Genetic Tools for Biofuels Production, pp. 103–121.
Source: ORCID
Added: June 21, 2019
2017 journal article
The proteome of higher plant mitochondria
Mitochondrion, 33, 22–37.
Contributors: R. Rao *, * , B. Thal*, H. Eubel *, J. Thelen * & I. Møller *
author keywords: (Plant) mitochondria; Proteomics; Localization prediction programs
MeSH headings : Computational Biology; Mass Spectrometry; Mitochondria / chemistry; Plant Proteins / analysis; Plants / chemistry; Proteome / analysis; Proteomics
TL;DR:
The proteomic studies have improved the understanding of basic mitochondrial functions, led to the discovery of new mitochondrial metabolic pathways and are helping us towards appreciating the dynamic role of the mitochondria in the responses of the plant cell to biotic and abiotic stress.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: June 21, 2019
2016 journal article
Proteomic response of the phytopathogen Phyllosticta citricarpa to antimicrobial volatile organic compounds from Saccharomyces cerevisiae
Microbiological Research.
author keywords: Antifungal; Citrus; Guignardia; Mechanism of action; Postharvest; Volatiles
MeSH headings : Amino Acids / metabolism; Anti-Infective Agents / pharmacology; Antifungal Agents / chemistry; Antifungal Agents / metabolism; Antifungal Agents / pharmacology; Ascomycota / drug effects; Ascomycota / genetics; Ascomycota / metabolism; Citric Acid Cycle / drug effects; Citrus / microbiology; Electrophoresis, Gel, Two-Dimensional; Fungal Proteins / drug effects; Fungal Proteins / isolation & purification; Fungal Proteins / metabolism; Glycolysis / drug effects; Nucleotides / metabolism; Plant Diseases / microbiology; Proteomics; Proton-Translocating ATPases / metabolism; Saccharomyces cerevisiae / chemistry; Tandem Mass Spectrometry; Volatile Organic Compounds / chemistry; Volatile Organic Compounds / metabolism; Volatile Organic Compounds / pharmacology
TL;DR:
The data indicate that antimicrobial VOCs interfere with essential metabolic pathways in P. citricarpa to prevent fungal growth, and proteins involved in metabolism, genetic information processing, cellular processes, and transport were the most strongly affected.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2016 journal article
Quantitative proteomic analysis of Araucaria angustifolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential
Journal of Proteomics, 130, 180–189.
Contributors: A. Santos*, P. Elbl*, B. Navarro *, L. Oliveira *, * , T. Balbuena *, E. Floh *
author keywords: Quantitative proteomics; Somatic embryogenesis; Conifer; Embryogenic potential; Molecular markers
MeSH headings : Carbohydrate Metabolism; Carbohydrates / chemistry; Databases, Protein; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation; Genotype; Plant Proteins / metabolism; Plant Somatic Embryogenesis Techniques; Proteome / metabolism; Proteomics / methods; RNA / chemistry; Reactive Oxygen Species / metabolism; Seeds / metabolism; Sequence Analysis, RNA; Tandem Mass Spectrometry; Tracheophyta / metabolism; Trypsin / chemistry
TL;DR:
Differences in protein abundance between the EC lines are discussed in terms of carbohydrate metabolism, cell division, defense response, gene expression, and response to reactive oxygen species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: ORCID
Added: June 21, 2019
2015 journal article
Comparative Proteomic Analysis of Developing Rhizomes of the Ancient Vascular PlantEquisetum hyemaleand Different Monocot Species
Journal of Proteome Research, 14(4), 1779–1791.
author keywords: rhizome development; proteomics; label free quantification
MeSH headings : Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Equisetum / genetics; Equisetum / metabolism; Plant Proteins / analysis; Plant Proteins / classification; Plant Proteins / metabolism; Poaceae / genetics; Poaceae / metabolism; Proteome / genetics; Proteome / metabolism; Proteomics / methods; Rhizome / genetics; Rhizome / metabolism; Species Specificity; Tandem Mass Spectrometry
TL;DR:
This work characterized the proteome of rhizome apical tips and elongation zones from different species using a GeLC-MS/MS (one-dimensional electrophoresis in combination with liquid chromatography coupled online with tandem mass spectrometry) spectral-counting proteomics strategy.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: June 21, 2019
2014 journal article
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement
BMC Plant Biology, 14(1).
Contributors: R. He *, * , J. Park*, M. Kim *, W. Nelson *, T. Balbuena *, M. Willer*, J. Crow *
author keywords: Transcriptomics; Proteomics; Metabolomics; Rhizome; Invasive species; Disease resistance; Rice blast; Rice
MeSH headings : Disease Resistance / genetics; Gene Expression Profiling; Gene Expression Regulation, Plant; Oryza / genetics; Oryza / metabolism; Oryza / physiology; Rhizome / genetics; Rhizome / metabolism; Rhizome / physiology; Transcriptome / genetics
TL;DR:
The presence of M. oryzae gene transcripts at a high level in apparently healthy plants suggests that red rice is resistant to this pathogen, and may be able to provide genes to cultivated rice that will enable resistance to rice blast disease.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2014 journal article
Isolation of Mitochondria from Potato Tubers
BIO-PROTOCOL, 4(17).
TL;DR:
This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the response of the immune system to E.coli.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2014 journal article
The potato tuber mitochondrial proteome
Plant Physiology, 164(2), 637–653.
Contributors: * , J. Havelund *, M. Chen *, R. Shyama Prasad Rao *, A. Rogowska-Wrzesinska *, O. Jensen *, D. Gang *, J. Thelen *, I. Møller *
MeSH headings : Arabidopsis / metabolism; Chromatography, Liquid; Databases, Protein; Fluorescence; Mitochondria / metabolism; Mitochondrial Proteins / chemistry; Mitochondrial Proteins / metabolism; Peptides / metabolism; Plant Epidermis / cytology; Plant Proteins / chemistry; Plant Proteins / metabolism; Plant Tubers / metabolism; Protein Processing, Post-Translational; Protein Transport; Proteome / chemistry; Proteome / metabolism; Solanum tuberosum / metabolism; Subcellular Fractions / metabolism; Tandem Mass Spectrometry; Tobacco / cytology
TL;DR:
This study reveals approximately 500 new or previously unconfirmed plant mitochondrial proteins and outlines a facile strategy for unbiased, near-comprehensive identification of mitochondria and their modified forms.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2013 journal article
Renal Proteome in Mice with Different Susceptibilities to Fluorosis
PLoS ONE, 8(1), e53261.
Ed(s): L. Martens
MeSH headings : Animals; Fluorides / metabolism; Fluorosis, Dental / genetics; Fluorosis, Dental / metabolism; Gene Expression Regulation; Kidney / metabolism; Male; Mice; Proteome / genetics; Proteome / metabolism; Tandem Mass Spectrometry
TL;DR:
Proteomic analysis was able to identify proteins potentially involved in metabolic handling of F and water that are differentially expressed or even not expressed in the strains evaluated, which can contribute to understanding the molecular mechanisms underlying genetic susceptibility to dental fluorosis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: June 21, 2019
2012 journal article
Large-Scale Proteome Comparative Analysis of Developing Rhizomes of the Ancient Vascular Plant Equisetum Hyemale
Frontiers in Plant Science, 3.
author keywords: rough horsetail; ferns; label-free proteomics; spectral counting
TL;DR:
This work characterized the proteome of E. hyemale rhizomes using a GeLC-MS spectral-counting proteomics strategy and found a vast dynamic range in the regulation of the 87 characteristic proteins and revealed the existence of nine major protein groups.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
2012 article
Strategies for Protein Separation
Salvato, F., Carvalho, M. C., & Lima Leite, A. (2012, February). Integrative Proteomics.
TL;DR:
The proteome has a dynamic and complex nature that is the result of many posttranslational modifications, molecular interactions, and a variety of proteins arising from alternative mRNA splicing, and current analytical strategies can reach different levels of resolution depending on the platform used.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: ORCID
Added: June 21, 2019
2012 journal article
Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues
World Journal of Microbiology and Biotechnology, 28(2), 463–472.
author keywords: Invertase; beta-Fructofuranosidase; Sucrose; Paecylomyces; Fungi
MeSH headings : Enzyme Stability; Fermentation / physiology; Fungal Proteins / chemistry; Fungal Proteins / isolation & purification; Fungal Proteins / metabolism; Hydrogen-Ion Concentration; Kinetics; Sucrose / metabolism; Temperature; beta-Fructofuranosidase / chemistry; beta-Fructofuranosidase / isolation & purification; beta-Fructofuranosidase / metabolism
Source: ORCID
Added: June 21, 2019
2010 journal article
Methods and strategies in proteomics and their applications in plants | Métodos e estratégias em proteômica e suas aplicações na área vegetal
Ciencia Rural, 40(3), 727–734. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953018551&partnerID=MN8TOARS
Source: ORCID
Added: June 21, 2019
2010 journal article
Métodos e estratégias em proteômica e suas aplicações na área vegetal
Ciência Rural, 40(3), 727–734.
author keywords: proteomics; protein analysis; interactomics; plants
TL;DR:
The implementation of mass spectrometry for peptides (MS) and amino acids (tandem MS or MS/MS) analysis allowed the identification of hundreds of proteins in single experiments, opening up new perspectives in plant biology with emphasis on studies of genetic variability, physiological stresses and plant development.
(via Semantic Scholar)
Source: ORCID
Added: June 21, 2019
Employment
Updated: October 31st, 2017 08:17
2014 - present
Universidade Estadual de Campinas Campinas, SP, BR
Postdoctor scientist
Plant Biology
2011 - 2013
University of Missouri Columbia Columbia, MO, US
Postdoctor scientist
Biochemistry
Education
Updated: April 14th, 2017 09:03
2005 - 2010
Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Piracicaba, SP, BR
PhD
Genetics
2000 - 2005
Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Piracicaba, SP, BR
Agronomic Engineer
Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.
Certain data included herein are derived from the Web of Science© and InCites© (2024) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.
