Works (135)

Updated: February 21st, 2024 05:02

2024 journal article

Surgical procedure of intratympanic injection and inner ear pharmacokinetics simulation in domestic pigs

FRONTIERS IN PHARMACOLOGY, 15.

By: A. Moatti n, S. Connard n, N. De Britto n, W. Dunn*, S. Rastogi n, M. Rai n, L. Schnabel n, F. Ligler* ...

author keywords: intratympanic; inner ear; fluid simulation; round window membrane; pigs; swine; pharmacokinetics
TL;DR: The potential of using pigs as a large animal model for studying inner ear pharmacokinetic studies of the inner ear using pigs is demonstrated and the diffusion time and drug concentrations reported align closely with the limited data available from human studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: February 19, 2024

2023 journal article

Assessment of drug permeability through an ex vivo porcine round window membrane model

ISCIENCE, 26(6).

By: A. Moatti n, D. Silkstone n, T. Martin n, K. Abbey n, K. Hutson*, D. Fitzpatrick*, C. Zdanski*, A. Cheng*, F. Ligler*, A. Greenbaum n

TL;DR: An ex vivo porcine RWM model, similar in structure and thickness to the human RWM, is developed, which provides a straightforward approach to developing effective and non-invasive delivery methods to the inner ear. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
5. Gender Equality (Web of Science)
Source: Web Of Science
Added: June 26, 2023

2023 journal article

Development of a light emitting device for the treatment of peritoneal car- cinomatosis of ovarian origin by intracavitary photodynamic therapy

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 41.

By: M. Overchuk, M. Ruhi, B. Rickard, F. Ligler & I. Rizvi

author keywords: Photodynamic therapy; peritoneal carcinomatosis; ovarian cancer
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: May 22, 2023

2023 journal article

Exposure to select PFAS and PFAS mixtures alters response to platinum-based chemotherapy in endometrial cancer cell lines

ENVIRONMENTAL HEALTH, 22(1).

By: B. Rickard*, M. Overchuk n, J. Tulino n, X. Tan, F. Ligler*, V. Bae-Jump*, S. Fenton*, I. Rizvi n

author keywords: PFAS; Endometrial cancer; Therapy resistance; Carboplatin; Cisplatin; Mitochondria
TL;DR: Investigation of the effect of environmental exposures, specifically PFAS, on platinum-based chemotherapy response and mitochondrial function in endometrial cancer suggests that PFAS exposure may affect endometrial cancer cell mitochondrial functioning. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: January 2, 2024

2023 journal article

Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning

PNAS NEXUS, 2(10).

By: Y. Wang n, S. Sadeghi n, A. Velayati n, R. Paul n, Z. Hetzler n, E. Danilov n, F. Ligler*, Q. Wei n

Ed(s): R. Reis

author keywords: smartphone videoscopy; V-chopper; time-resolved; lifetime imaging; machine learning
TL;DR: A cost-effective and miniaturized smartphone lifetime imaging system integrated with a pulsed ultraviolet (UV) light-emitting diode (LED) for 2D luminescence lifetime imaging using a videoscopy-based virtual chopper (V-chopper) mechanism combined with machine learning. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 6, 2023

2023 journal article

Targeted PDT Combinations to Overcome Fluid Shear Stress-induced Plat- inum Resistance in Ovarian Cancer

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 41.

By: M. Overchuk n, M. Ruhi n, B. Rickard n, F. Ligler* & I. Rizvi n

author keywords: photodynamic therapy; ovarian cancer; benzoporphyrin derivative; chemoresistance; shear stress
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: May 22, 2023

2023 journal article

Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models

STAR PROTOCOLS, 4(2).

By: A. Moatti n, Y. Cai n, C. Li n, K. Popowski n, K. Cheng n, F. Ligler*, A. Greenbaum n

author keywords: imaged cochleae using open -source software.; several mm; larger animal inner; open -source software
TL;DR: A protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models is presented and a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software is detailed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 5, 2023

2022 article

Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells

Agarwalla, P., Ogunnaike, E. A., Ahn, S., Froehlich, K. A., Jansson, A., Ligler, F. S., … Brudno, Y. (2022, March 24). NATURE BIOTECHNOLOGY, Vol. 3.

By: P. Agarwalla n, E. Ogunnaike*, S. Ahn*, K. Froehlich n, A. Jansson n, F. Ligler n, G. Dotti*, Y. Brudno n

MeSH headings : Animals; Antigens, CD19; B-Lymphocytes; Humans; Immunotherapy, Adoptive / methods; Leukocytes, Mononuclear / metabolism; Mice; Receptors, Antigen, T-Cell; T-Lymphocytes
TL;DR: An implantable Multifunctional Alginate Scaffold for T Cell Engineering and Release (MASTER) that streamlines in vivo CAR-T cell manufacturing and reduces processing time to a single day is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 18, 2022

2022 journal article

Flexible sensor patch for continuous carbon dioxide monitoring

FRONTIERS IN CHEMISTRY, 10.

By: Z. Hetzler n, Y. Wang n, D. Krafft n, S. Jamalzadegan n, L. Overton n, M. Kudenov n, F. Ligler*, Q. Wei n

author keywords: CO2; in-line sensor; flexible sensor; process analytical technology; cell culture; biomanufacturing
Sources: Web Of Science, NC State University Libraries
Added: March 6, 2023

2022 journal article

Multilayer microfluidic platform for the study of luminal, transmural, and interstitial flow

BIOFABRICATION, 14(2).

By: G. Lee n, S. Huang n, W. Aw n, M. Rathod n, C. Cho n, F. Ligler n, W. Polacheck n

author keywords: microfluidics; vascular biology; interstitial flow; hemodynamics; biofluid mechanics; mechanotransduction
MeSH headings : Lymphatic Vessels; Microfluidics / methods; Stress, Mechanical
TL;DR: A multi-layer microfluidic platform (MμLTI-Flow) is introduced that enables the culture of engineered blood and lymphatic microvessels and independent control of blood, lymphatic, and IFPs and will provide for analysis of key fluid mechanical parameters and cellular mechanisms that contribute to diseases in which fluid imbalances play a role in progression, including lymphedema and solid cancer. (via Semantic Scholar)
Source: Web Of Science
Added: January 31, 2022

2022 journal article

Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging

ISCIENCE, 25(8).

By: A. Moatti n, C. Li n, S. Sivadanam n, Y. Cai n, J. Ranta n, J. Piedrahita n, A. Cheng*, F. Ligler n, A. Greenbaum n

TL;DR: Data reveal that hair cell organization, inner ear morphology, cellular cartography in the organ of Corti, and spatiotemporal expression of LGR5 are dynamic over developmental stages in a pattern not previously documented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 19, 2022

2021 article

Enhancement of Bone Regeneration Through the Converse Piezoelectric Effect, A Novel Approach for Applying Mechanical Stimulation

Carter, A., Popowski, K., Cheng, K., Greenbaum, A., Ligler, F. S., & Moatti, A. (2021, September 24). BIOELECTRICITY, Vol. 9.

By: A. Carter n, K. Popowski n, K. Cheng n, A. Greenbaum n, F. Ligler n & A. Moatti n

author keywords: converse piezoelectric effect; mechanical stimulation; bone regeneration
TL;DR: The potential of the converse piezoelectric effect to enhance bone regeneration is highlighted by discussing the activation of beneficial cellular pathways, the properties of piezOElectric biomaterials, and the potential for the more effective administration of the Converse pieZoelectrics effect using wireless control. (via Semantic Scholar)
UN Sustainable Development Goal Categories
5. Gender Equality (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 25, 2021

2021 journal article

Fibrin gel enhances the antitumor effects of chimeric antigen receptor T cells in glioblastoma

SCIENCE ADVANCES, 7(41).

By: E. Ogunnaike n, A. Valdivia*, M. Yazdimamaghani*, E. Leon*, S. Nandi n, H. Hudson*, H. Du*, S. Khagi* ...

UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: October 26, 2021

2021 journal article

Microphysiological System for High-Throughput Computer Vision Measurement of Microtissue Contraction

ACS SENSORS, 6(3), 985–994.

author keywords: high throughput; 3D culture; tissue function; tissue contraction; computer vision
MeSH headings : Computers; Fibroblasts; High-Throughput Screening Assays; Humans; Image Processing, Computer-Assisted
TL;DR: An inexpensive, versatile, high-throughput platform to measure microtissue contraction in a 96-well plate configuration using one-step batch imaging and an algorithm uses pixel-based image analysis and computer vision techniques for the accurate multiwell quantification of positional changes in the optical microprobes caused by the contraction of the microtissues. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: May 10, 2021

2021 review

Review of analytical performance of COVID-19 detection methods

[Review of ]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 413(1), 35–48.

By: B. Giri*, S. Pandey*, R. Shrestha*, K. Pokharel*, F. Ligler n & B. Neupane*

author keywords: Coronavirus; SARS-CoV-2; COVID-19; RT-PCR; Immunoassays; Disease diagnosis
MeSH headings : Antibodies, Viral / analysis; COVID-19 / diagnosis; COVID-19 / epidemiology; COVID-19 / virology; Humans; Pandemics; RNA, Viral / genetics; SARS-CoV-2 / genetics; SARS-CoV-2 / immunology; SARS-CoV-2 / isolation & purification; Sensitivity and Specificity
TL;DR: Recent reported diagnostic methods for SARS-CoV-2 have varying throughput, batching capacity, requirement of infrastructure setting, analytical performance, and turnaround times ranging from a few minutes to several hours. (via Semantic Scholar)
Source: Web Of Science
Added: October 5, 2020

2021 journal article

Synthesis of sonicated fibrin nanoparticles that modulate fibrin clot polymerization and enhance angiogenic responses

Colloids and Surfaces B: Biointerfaces, 204, 111805.

By: C. Roosa n, I. Muhamed n, A. Young n, K. Nellenbach n, M. Daniele n, F. Ligler n, A. Brown n

author keywords: Fibrin; Nanoparticles; Wound healing; Regenerative medicine; Fibrin sealant
MeSH headings : Fibrin; Fibrin Tissue Adhesive; Nanoparticles; Polymerization; Wound Healing
TL;DR: It is shown that SFBNs may be a promising wound healing therapy that can be easily produced and delivered in a flowable formulation and support cell migration in an in vitro angiogenic sprouting assay, relevant for wound healing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: July 19, 2021

2020 journal article

Cardiac Stromal Cell Patch Integrated with Engineered Microvessels Improves Recovery from Myocardial Infarction in Rats and Pigs

ACS BIOMATERIALS SCIENCE & ENGINEERING, 6(11), 6309–6320.

By: T. Su n, K. Huang n, K. Mathews n, V. Scharf n, S. Hu n, Z. Li n, B. Frame*, J. Cores n ...

author keywords: microfluidics; cardiac patch; cardiac stromal cells; myocardial infarction; porcine model
MeSH headings : Animals; Microvessels; Myocardial Infarction / therapy; Myocytes, Cardiac; Neovascularization, Physiologic; Rats; Stromal Cells; Swine
TL;DR: It is shown that BMV-CSC patch transplantation significantly promoted cardiac function, reduced scar size, increased viable myocardial tissue, promoted neovascularization, and suppressed inflammation in rat and porcine MI models, demonstrating enhanced therapeutic efficacy compared to conventional cardiac stromal cell patches. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 11, 2020

2020 journal article

High-Throughput Manufacture of 3D Fiber Scaffolds for Regenerative Medicine

TISSUE ENGINEERING PART C-METHODS, 26(7), 364–374.

author keywords: 3D printing; melt blowing; fibers; scaffolds
MeSH headings : Animals; Biocompatible Materials / chemistry; Dogs; Hernia / therapy; Herniorrhaphy / methods; Polymers / chemistry; Printing, Three-Dimensional / instrumentation; Regenerative Medicine; Tissue Engineering / methods; Tissue Scaffolds / chemistry
TL;DR: The 3DMB system described in this study is modified from larger nonwovens manufacturing machinery to accommodate the lower volume, high-cost polymers used for tissue engineering and implantable biomedical devices and has a fiber collection component that uses adaptable robotics to create scaffolds with predetermined geometries. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 10, 2020

2020 journal article

Microfluidics for the study of mechanotransduction

JOURNAL OF PHYSICS D-APPLIED PHYSICS, 53(22).

author keywords: microfluidics; mechanotransduction; mechanobiology; microfabrication; cell adhesion; biofluids; microdevices
TL;DR: An overview of the main mechanotransduction pathways that function at sites of cell adhesion are given, and examples of how microfluidics can be used to apply both solid forces and fluid forces to cells are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: April 20, 2020

2020 journal article

Scaffold-Mediated Static Transduction of T Cells for CAR-T Cell Therapy

ADVANCED HEALTHCARE MATERIALS, 9(14).

By: P. Agarwalla n, E. Ogunnaike*, S. Ahn*, F. Ligler n, G. Dotti* & Y. Brudno n

author keywords: alginate scaffold; CAR-T cells; cell therapy; immunotherapy; viral transduction
MeSH headings : Antigens, CD19; Cell- and Tissue-Based Therapy; Humans; Immunotherapy, Adoptive; Receptors, Chimeric Antigen / genetics; T-Lymphocytes
TL;DR: The data show that macroporous alginate scaffolds serve as an attractive alternative to current transduction protocols and have high potential for clinical translation to genetically modify T cells for adoptive cellular therapy. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 29, 2020

2020 journal article

Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

BIOMEDICAL OPTICS EXPRESS, 11(11), 6181–6196.

By: A. Moatti n, Y. Cai n, C. Li n, T. Sattler n, L. Edwards n, J. Piedrahita n, F. Ligler n, A. Greenbaum n

TL;DR: 3D microscopic analysis was performed on an intact cochlea of newborn and juvenile pigs with a volume up to ∼ 250 mm3 using the BoneClear tissue clearing technique, which renders the bone transparent, to support the growing effort to regenerate cochlear tissues and assist with basic research to advance cures for hearing impairments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 11, 2020

2019 journal article

Biomimetics: Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury (Adv. Funct. Mater. 4/2019)

Advanced Functional Materials, 29(4), 1970019.

By: T. Su n, K. Huang n, H. Ma*, H. Liang n, P. Dinh n, J. Chen n, D. Shen n, T. Allen n ...

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2019 journal article

Characterization of glass frit capillary pumps for microfluidic devices

MICROFLUIDICS AND NANOFLUIDICS, 23(5).

By: M. Rich n, O. Mohd n, F. Ligler n & G. Walker*

author keywords: Porous; Glass; Capillary forces; Pump; Microfluidic; Point-of-care
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: May 6, 2019

2019 journal article

Fibrin Nanoparticles Coupled with Keratinocyte Growth Factor Enhance the Dermal Wound-Healing Rate

ACS Applied Materials & Interfaces, 11(4), 3771–3780.

By: I. Muhamed n, E. Sproul n, F. Ligler n & A. Brown n

author keywords: fibrin nanoparticles; tissue regeneration; cell migration; microfluidics; wound healing; biomaterials; cell migration; in vitro 3D wound model and in vivo dermal wound model
MeSH headings : Animals; Biocompatible Materials / chemistry; Cell Movement / drug effects; Fibrin / chemistry; Fibroblast Growth Factor 7 / chemistry; Fibroblast Growth Factor 7 / therapeutic use; Mice; Microfluidics; Nanoparticles / chemistry; Wound Healing / drug effects
TL;DR: FBNs described here are constructed from physiologically relevant fibrin concentrations that support new tissue and dermal wound scaffold formation when coupled with growth factors and can be potentially administered with other tissue-healing factors and inflammatory mediators to improve wound- healing outcomes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 18, 2019

2019 journal article

Lighting Up Biosensors: Now and the Decade To Come

ANALYTICAL CHEMISTRY, 91(14), 8732–8738.

By: F. Ligler n & J. Gooding*

MeSH headings : Animals; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Equipment Design; Humans; Optical Devices; Optics and Photonics / instrumentation; Optics and Photonics / methods
TL;DR: The innovative approaches to overcoming existing hurdles to practical biosensor designs are reviewed and potential areas for future breakthroughs in optical biosensor technology are explored. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 5, 2019

2019 journal article

Paper-based passive pumps to generate controllable whole blood flow through microfluidic devices

LAB ON A CHIP, 19(22), 3787–3795.

By: M. Sotoudegan n, O. Mohd*, F. Ligler n & G. Walker*

MeSH headings : Animals; Blood Component Removal / instrumentation; Microfluidic Analytical Techniques / instrumentation; Paper; Particle Size; Porosity; Swine
TL;DR: The promising performance of grooved paper pumps with whole blood offers potential applications of these small, disposable pumps in point-of-care diagnostics in which time is crucial and access to external power is limited. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: November 25, 2019

2019 journal article

Photothermal Therapy Promotes Tumor Infiltration and Antitumor Activity of CAR T Cells

ADVANCED MATERIALS, 31(23).

By: Q. Chen n, Q. Hu n, E. Dukhovlinova*, G. Chen*, S. Ahn*, C. Wang n, E. Ogunnaike n, F. Ligler n, G. Dotti*, Z. Gu n

author keywords: CAR T cells; cell therapy; drug delivery; immunotherapy; photothermal therapy
MeSH headings : Animals; Antigens / metabolism; Cell Line, Tumor; Chondroitin Sulfate Proteoglycans / metabolism; Combined Modality Therapy; Female; Heterografts; Humans; Hyperthermia, Induced; Immunotherapy, Adoptive / methods; Indocyanine Green / chemistry; Melanoma / pathology; Melanoma / therapy; Membrane Proteins / metabolism; Mice, SCID; Nanoparticles / chemistry; Photosensitizing Agents / chemistry; Phototherapy / methods; Polylactic Acid-Polyglycolic Acid Copolymer / chemistry; Proteoglycans / metabolism; Skin Neoplasms / pathology; Skin Neoplasms / therapy; T-Lymphocytes / metabolism; T-Lymphocytes / transplantation; Tumor Microenvironment
TL;DR: It is found that the chondroitin sulfate proteoglycan‐4 (CSPG4)‐specific CAR T cells infused in Nod scid gamma mice engrafted with the human melanoma WM115 cell line have superior antitumor activity after photothermal ablation of the tumor. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: July 22, 2019

2019 journal article

Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury

ADVANCED FUNCTIONAL MATERIALS, 29(4).

By: T. Su n, K. Huang n, H. Ma*, H. Liang n, P. Dinh n, J. Chen n, D. Shen n, T. Allen n ...

author keywords: biomimetics; drug delivery; ischemic injury; platelets; stromal cell secretome
TL;DR: A platelet‐inspired nanocell (PINC) that incorporates both prostaglandin E2 (PGE2)‐modified platelet membrane and cardiac stromal cell‐secreted factors to target the heart after I/R injury is introduced, which represents a promising therapeutic delivery platform for treating myocardial ischemia/reperfusion injury. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 4, 2019

2018 journal article

Cardiac Stem Cell Patch Integrated with Microengineered Blood Vessels Promotes Cardiomyocyte Proliferation and Neovascularization after Acute Myocardial Infarction

ACS APPLIED MATERIALS & INTERFACES, 10(39), 33088–33096.

By: T. Su n, K. Huang n, M. Daniele n, M. Hensley n, A. Young n, J. Tang n, T. Allen n, A. Vandergriff n ...

author keywords: microvessels; hydrodynamic focusing; cardiac stem cells; cardiomyocyte proliferation; angiogenesis; myocardial infarction
MeSH headings : Animals; Cells, Cultured; Female; Human Umbilical Vein Endothelial Cells; Humans; Microfluidic Analytical Techniques; Myocardial Infarction / therapy; Myocardium / cytology; Myocytes, Cardiac / cytology; Myocytes, Cardiac / physiology; Neovascularization, Physiologic / physiology; Rats; Rats, Nude; Stem Cells / cytology; Stem Cells / physiology
TL;DR: It is shown that the endothelialized BMVs mimicked the natural architecture and function of capillaries and that the resultant vascularized cardiac patch (BMV-CSC patch) exhibited equivalent release of paracrine factors compared to those of coculture of genuine human CSCs and HUVECs after 7 days of in vitro culture. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 29, 2018

2018 article

THE NAI FELLOW PROFILE: AN INTERVIEW WITH DR. FRANCES LIGLER

TECHNOLOGY AND INNOVATION, Vol. 19, pp. 645–651.

By: F. Ligler* & K. Macuare

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Hypoxia and H2O2 Dual-Sensitive Vesicles for Enhanced Glucose-Responsive Insulin Delivery

NANO LETTERS, 17(2), 733–739.

By: J. Yu n, C. Qian n, Y. Zhang n, Z. Cui n, Y. Zhu n, Q. Shen*, F. Ligler n, J. Buse*, Z. Gu n

author keywords: Drug delivery; diabetes; insulin; glucose-responsive; hypoxia-sensitive; H2O2-sensitive
MeSH headings : Animals; Blood Glucose / metabolism; Cell Hypoxia; Diabetes Mellitus, Experimental / drug therapy; Diabetes Mellitus, Experimental / metabolism; Diabetes Mellitus, Type 1 / drug therapy; Diabetes Mellitus, Type 1 / metabolism; Drug Carriers / chemistry; Drug Liberation; Glucose / metabolism; Glucose Oxidase / metabolism; HeLa Cells; Humans; Hydrogen Peroxide / metabolism; Hypoglycemic Agents / administration & dosage; Hypoglycemic Agents / chemistry; Insulin / administration & dosage; Insulin / chemistry; Male; Mice, Inbred C57BL; Nitroimidazoles / chemistry; Oxidation-Reduction; Peptides / chemistry; Polyethylene Glycols / chemistry
TL;DR: In vivo experiments indicated that this smart insulin patch could efficiently regulate the blood glucose in the chemically induced type 1 diabetic mice for 10 h and eliminate the excess H2O2, which may lead to free radical-induced damage to skin tissue during the long-term usage and reduce the activity of GOx. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Leveraging H2O2 Levels for Biomedical Applications

Advanced Biosystems, 1(9), 1700084.

By: J. Wang n, Y. Zhang n, E. Archibong n, F. Ligler n & Z. Gu n

author keywords: diagnosis; drug delivery; H2O2-responsive; oxidation-responsive; polymers
TL;DR: The development of H2O2‐responsive functional groups for constructing H1N1‐responsive formulations, as well as the guidance for designing suitable formulations to treat each specific pathological condition are overviewed. (via Semantic Scholar)
Source: Crossref
Added: November 30, 2020

2017 journal article

Microfabricated blood vessels undergo neoangiogenesis

BIOMATERIALS, 138, 142–152.

By: K. DiVito*, M. Daniele n, S. Roberts*, F. Ligler n & A. Adams*

author keywords: Sheath-flow microfluidics; Blood vessel; Angiogenesis; Anastomoses; Perfusion
MeSH headings : Capillaries / chemistry; Coculture Techniques; Endothelial Cells / cytology; Endothelial Cells / metabolism; Extracellular Matrix Proteins / metabolism; Feeder Cells; Fibroblasts / cytology; Fibroblasts / metabolism; Gelatin / chemistry; Humans; Hydrogels / chemistry; Microfluidics / methods; Microtechnology / methods; Microvessels / growth & development; Microvessels / ultrastructure; Neovascularization, Physiologic; Perfusion; Pericytes / cytology; Pericytes / metabolism; Polyhydroxyethyl Methacrylate / chemistry; Primary Cell Culture; Tissue Engineering / methods; Umbilical Veins / cytology
TL;DR: Ultraviolet (UV) photo-crosslinkable poly(ethylene glycol) and gelatin methacrylate polymers used in combination with sheath-flow microfluidics allow for the fabrication of small diameter blood vessels which undergo neoangiogenesis as well as other developmental processes associated with normal human blood vessel maturation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Modular pumps as programmable hydraulic batteries for microfluidic devices

TECHNOLOGY, 5(1), 21–30.

By: B. Cummins n, R. Chinthapatla n, B. Lenin n, F. Ligler n & G. Walker n

author keywords: Paper Microfluidics; Capillary Pump; Microfluidic; Lateral Flow; Microfluidic Pump; Point of Care
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Synthetic beta cells for fusion-mediated dynamic insulin secretion

Nature Chemical Biology, 14(1), 86–93.

MeSH headings : Animals; Artificial Cells; Biomimetic Materials / chemistry; Blood Glucose / analysis; Cell Engineering / methods; Diabetes Mellitus, Experimental / blood; Diabetes Mellitus, Experimental / drug therapy; Diabetes Mellitus, Type 1 / blood; Diabetes Mellitus, Type 1 / drug therapy; Hydrogen-Ion Concentration; Insulin / blood; Insulin / metabolism; Insulin Secretion; Insulin-Secreting Cells / metabolism; Male; Membrane Fusion; Mice, Inbred C57BL
TL;DR: The construction of artificial beta cells with a multicompartmental 'vesicles-in-vesicle' superstructure equipped with a glucose-metabolism system and membrane-fusion machinery is described, which can effectively distinguish between high and normal glucose levels. (via Semantic Scholar)
Sources: Crossref, Web Of Science
Added: August 6, 2018

2017 journal article

Time-Dependent Model for Fluid Flow in Porous Materials with Multiple Pore Sizes

Analytical Chemistry, 89(8), 4377–4381.

By: B. Cummins n, R. Chinthapatla n, F. Ligler n & G. Walker n

TL;DR: A new transport model is presented that incorporates a range of pore sizes to more accurately predict the capillary transport of fluid in porous materials. (via Semantic Scholar)
Sources: Crossref, Web Of Science
Added: August 6, 2018

2017 journal article

“Data characterizing microfabricated human blood vessels created via hydrodynamic focusing”

“Data characterizing microfabricated human blood vessels created via hydrodynamic focusing.” Data in Brief, 14, 156–162.

By: K. DiVito*, M. Daniele n, S. Roberts*, F. Ligler n & A. Adams*

author keywords: Microfluidics; Ge1MA; Endothelial cell; Blood vessel; Microvessel; Organ-on-chip
TL;DR: Data is reported regarding the construction of blood vessels including cellular composition and cell positioning within the engineered vascular construct as well as functional aspects of the tissues. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2016 journal article

Evanescent wave fluorescence biosensors: Advances of the last decade

Biosensors and Bioelectronics, 76, 103–112.

By: C. Taitt*, G. Anderson* & F. Ligler n

author keywords: Optical biosensor; Evanescent wave; Fluorescence; Applications
MeSH headings : Biosensing Techniques / methods; Biosensing Techniques / trends; Clinical Laboratory Techniques / methods; Fiber Optic Technology / methods; Fluorescence; Humans
TL;DR: This review will focus on new technological developments as well as technology commercialized in just the last decade in the subfield of evanescent wave fluorescence biosensors. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2016 journal article

Mechanical and Vascular Cues Synergistically Enhance Osteogenesis in Human Mesenchymal Stem Cells

TISSUE ENGINEERING PART A, 22(15-16), 997–1005.

By: A. Steward n, J. Cole n, F. Ligler n & E. Loboa n

Contributors: A. Steward n, J. Cole n, F. Ligler n & E. Loboa n

MeSH headings : Adolescent; Adult; Antigens, Differentiation / biosynthesis; Coculture Techniques; Endothelial Cells / cytology; Endothelial Cells / metabolism; Female; Humans; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Neovascularization, Physiologic; Osteogenesis
TL;DR: The concurrent application of strain to an MSC-EC co- culture resulted in a significant increase in calcium accretion and mineral deposition, suggesting that co-culture and strain synergistically enhance osteogenesis. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures

Biomicrofluidics, 10(5), 054109.

By: S. Roberts*, K. DiVito*, F. Ligler n, A. Adams* & M. Daniele n

TL;DR: An inclusive on-chip platform that is capable of maintaining laminar flow through porous biosynthetic microvessels and characterize the diffusion of small molecules from the perfusate, across the microvessel wall, and into the matrix of a 3D cell culture is developed. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 6, 2018

2016 journal article

Nanosecond Time-Resolution Study of Gold Nanorod Rotation at the Liquid-Solid Interface

CHEMPHYSCHEM, 17(14), 2218–2224.

By: B. Neupane n, F. Chen n, Y. Wei n, N. Fang*, F. Ligler n & G. Wang n

author keywords: adsorption; fluctuation correlation spectroscopy; gold nanorods; rotation; surface plasmon resonance
TL;DR: This nanosecond time-resolution study reveals that the apparent continuous rotation of gold nanorods actually consists of numerous fast, intermittent rotations or transitions between a small number of weakly immobilized states, with the particle resting in the immobilization states most of the time. (via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Point-of-care diagnostics for niche applications

Biotechnology Advances, 34(3), 161–176.

By: B. Cummins n, F. Ligler n & G. Walker n

author keywords: Point-of-care; Diagnostics; Space travel; Sports medicine; Veterinary; Emergency care; Operating room
MeSH headings : Animals; Emergency Service, Hospital / organization & administration; Humans; Operating Rooms / organization & administration; Point-of-Care Systems / statistics & numerical data; Point-of-Care Systems / trends; Space Flight; Sports Medicine; Veterinary Medicine
TL;DR: This review discusses five areas relevant to human and animal health where increased attention could produce significant impact: veterinary medicine, space travel, sports medicine, emergency Medicine, emergency medicine, and operating room efficiency. (via Semantic Scholar)
Sources: Crossref, Web Of Science
Added: August 6, 2018

2016 journal article

Signal amplification strategies for microfluidic immunoassays

TrAC Trends in Analytical Chemistry, 79, 326–334.

author keywords: Limit of detection; Sensitivity; Biomarkers; Preconcentration; Magnetic beads; Immuno-PCR
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Crossref
Added: February 24, 2020

2015 journal article

3D hydrodynamic focusing microfluidics for emerging sensing technologies

Biosensors and Bioelectronics, 67, 25–34.

By: M. Daniele*, D. Boyd*, D. Mott* & F. Ligler n

author keywords: Microfluidics; Sensors; Hydrodynamic focusing; Flow cytometry; Microfluidic fabrication
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Cell Separation; Flow Cytometry; Hydrodynamics; Microfluidics
TL;DR: The underlying principles for hydrodynamic focusing in three dimensions (3D) using miscible fluids and the application of these principles for creation of bios Sensors, separation of cells and particles for sample manipulation, and fabrication of materials that could be used for biosensors are explored. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2015 journal article

A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment

NANOSCALE, 7(28), 12096–12103.

By: D. Pacardo n, B. Neupane n, S. Rikard n, Y. Lu n, R. Mo n, S. Mishra n, J. Tracy n, G. Wang n, F. Ligler n, Z. Gu n

Contributors: D. Pacardo n, B. Neupane n, S. Rikard n, Y. Lu n, R. Mo n, S. Mishra n, J. Tracy n, G. Wang n, F. Ligler n, Z. Gu n

MeSH headings : Antineoplastic Agents / chemistry; Antineoplastic Agents / pharmacology; Drug Screening Assays, Antitumor; Gold / chemistry; Gold / pharmacology; HeLa Cells; Humans; Infrared Rays; Nanotubes / chemistry; Neoplasms / drug therapy; Photochemotherapy
TL;DR: This multifunctional AuNR-based system represents a novel theranostic strategy for spatiotemporal delivery of anticancer therapeutics and demonstrates enhanced anticancer efficacy through the synergistic combination of promoted drug release upon ultraviolet (UV) light irradiation and photothermal therapy upon infrared (IR) irradiation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

A temperature microsensor for measuring laser-induced heating in gold nanorods

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(3), 719–725.

By: D. Pacardo n, B. Neupane n, G. Wang n, Z. Gu n, G. Walker n & F. Ligler n

author keywords: Microsensor; Gold nanorods; Temperature; Nanoparticles; Microfluidics
MeSH headings : Chemistry Techniques, Analytical / instrumentation; Chemistry Techniques, Analytical / methods; Equipment Design; Gold; Heating; Hot Temperature; Lasers; Microtechnology; Nanotubes / analysis
TL;DR: The results demonstrate the suitability of this simple temperature microsensor for determining laser-induced heating profiles of metallic nanomaterials; such measurements will be essential for optimizing therapeutic and catalytic applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Continuous-wave stimulated emission depletion microscope for imaging actin cytoskeleton in fixed and live cells

Sensors (Basel, Switzerland), 15(9), 24178–24190.

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Microfluidic strategies for design and assembly of microfibers and nanofibers with tissue engineering and regenerative medicine applications

Advanced Healthcare Materials, 4(1).

By: M. Daniele, D. Boyd, A. Adams & F. Ligler

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Microfluidics: Microfluidic Strategies for Design and Assembly of Microfibers and Nanofibers with Tissue Engineering and Regenerative Medicine Applications (Adv. Healthcare Mater. 1/2015)

Advanced Healthcare Materials, 4(1), 2–2.

By: M. Daniele*, D. Boyd*, A. Adams* & F. Ligler n

Source: Crossref
Added: December 31, 2020

2015 journal article

Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(27), 8260–8265.

By: J. Yu n, Y. Zhang n, Y. Ye n, R. DiSanto n, W. Sun n, D. Ranson n, F. Ligler n, J. Buse*, Z. Gu n

author keywords: diabetes; drug delivery; glucose-responsive; hypoxia-sensitive; microneedle
MeSH headings : Animals; Blood Glucose / analysis; Diabetes Mellitus, Experimental / blood; Diabetes Mellitus, Experimental / drug therapy; Diabetes Mellitus, Type 1 / blood; Diabetes Mellitus, Type 1 / drug therapy; Drug Delivery Systems / instrumentation; Drug Delivery Systems / methods; Glucose / metabolism; Glucose Oxidase / metabolism; Humans; Hyaluronic Acid / chemistry; Hyaluronic Acid / metabolism; Hypoglycemic Agents / administration & dosage; Hypoglycemic Agents / chemistry; Hypoxia / metabolism; Insulin / administration & dosage; Insulin / chemistry; Insulin / metabolism; Male; Mice, Inbred C57BL; Microscopy, Electron, Transmission; Molecular Structure; Oxidation-Reduction; Reproducibility of Results
TL;DR: The “smart insulin patch” with a new enzyme-based glucose-responsive mechanism can regulate the blood glucose of type 1 diabetic mice to achieve normal levels, with faster responsiveness compared with the commonly used pH-sensitive formulations, and can avoid the risk of hypoglycemia. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 review

Programmable nanomedicine: synergistic and sequential drug delivery systems

[Review of ]. NANOSCALE, 7(8), 3381–3391.

By: D. Pacardo n, F. Ligler n & Z. Gu n

MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Cell Line, Tumor; DNA / chemistry; Delayed-Action Preparations; Drug Carriers; Drug Delivery Systems; Drug Resistance, Neoplasm; Humans; Microscopy, Electron, Transmission; Nanomedicine / methods; Nanoparticles / chemistry; Neoplasms / drug therapy; Neoplasms / pathology
TL;DR: This minireview discusses the current advances in programmable nanocarriers for cancer therapy with particular emphasis on synergistic and sequential drug delivery systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

The Scope of Analytical Chemistry

Analytical Chemistry, 87(13), 6425–6425.

By: J. Sweedler, D. Armstrong, Y. Baba, G. Desmet, N. Dovichi, A. Ewing, C. Fenselau, R. Kennedy ...

TL;DR: This editorial discusses what to look for when evaluating manuscript submissions to Analytical Chemistry, and some key factors to consider when deciding to submit an article to the journal. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2015 journal article

Transformable liquid-metal nanomedicine

NATURE COMMUNICATIONS, 6(1).

By: Y. Lu n, Q. Hu n, Y. Lin n, D. Pacardo n, C. Wang n, W. Sun n, F. Ligler n, M. Dickey n, Z. Gu*

MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Antineoplastic Agents / chemistry; Cell Line, Tumor; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Drug Carriers / chemistry; Drug Delivery Systems / instrumentation; Drug Delivery Systems / methods; Female; Gallium / chemistry; Humans; Indium / chemistry; Metal Nanoparticles / chemistry; Mice; Mice, Inbred BALB C; Nanomedicine / instrumentation; Nanomedicine / methods; Neoplasms / drug therapy
TL;DR: A transformable liquid-metal nanomedicine, based on a core–shell nanosphere composed of a liquid-phase eutectic gallium-indium core and a thiolated polymeric shell, providing a new strategy for engineering theranostic agents with low toxicity. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Facile Fabrication of Color Tunable Film and Fiber Nanocomposites via Thiol Click Chemistry

MACROMOLECULES, 47(2), 695–704.

By: D. Boyd*, J. Naciri*, J. Fontana*, D. Pacardo n, A. Shields*, J. Verbarg*, C. Spillmann*, F. Ligler n

Source: Web Of Science
Added: August 6, 2018

2014 journal article

Interpenetrating networks based on gelatin methacrylamide and PEG formed using concurrent thiol click chemistries for hydrogel tissue engineering scaffolds

Biomaterials, 35(6), 1845–1856.

By: M. Daniele*, A. Adams*, J. Naciri*, S. North* & F. Ligler*

author keywords: Interpenetrating network (IPN); Extracellular matrix; Thiol-yne; Click chemistry; Gelatin; Poly(ethylene glycol)
MeSH headings : Acrylamides / chemistry; Click Chemistry; Extracellular Matrix / chemistry; Gelatin / chemistry; Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry; Polyethylene Glycols / chemistry; Tissue Engineering / methods; Tissue Scaffolds / chemistry
TL;DR: The development of a bio/synthetic interpenetrating network (BioSINx), containing gelatin methacrylamide polymerized within a poly(ethylene glycol) (PEG) framework to form a mechanically robust network capable of supporting both internal cell encapsulation and surface cell adherence, leading to enhanced cytocompatibility for both cell adhesion and encapsulation. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2014 journal article

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (83).

By: D. Boyd*, A. Adams*, M. Daniele* & F. Ligler n

author keywords: Bioengineering; Issue 83; hydrodynamic focusing; polymer fiber; biohybrid; microfabrication; sheath flow; click chemistry
MeSH headings : Click Chemistry; Hydrogels / chemistry; Hydrophobic and Hydrophilic Interactions; Liquid Crystals / chemistry; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Photochemical Processes; Polymers / chemistry; Viscosity
TL;DR: A “sheath” fluid passing through a microfluidic channel at low Reynolds number can be directed around another “core” stream and used to dictate the shape as well as the diameter of a core stream by matching the viscosity and hydrophilicity of the sheath and core fluids. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Microfluidic Strategies for Design and Assembly of Microfibers and Nanofibers with Tissue Engineering and Regenerative Medicine Applications

Advanced Healthcare Materials, 4(1), 11–28.

By: M. Daniele*, D. Boyd*, A. Adams* & F. Ligler n

MeSH headings : Animals; Humans; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microfluidic Analytical Techniques / trends; Microfluidics / methods; Microfluidics / trends; Portraits as Topic
TL;DR: This Progress Report summarizes advanced microfluidic methods for the fabrication of both microscale and nanoscale fibers and illustrates how different methods are enabling new biomedical applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: August 28, 2020

2014 journal article

Microfluidic fabrication of multiaxial microvessels via hydrodynamic shaping

RSC Adv., 4(45), 23440–23446.

By: M. Daniele*, K. Radom*, F. Ligler n & A. Adams*

UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2014 review

Review of recent developments in stimulated emission depletion microscopy: Applications on cell imaging

[Review of ]. Journal of Biomedical Optics, 19(8).

By: B. Neupane, F. Ligler & G. Wang

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Self-folded redox/acid dual-responsive nanocarriers for anticancer drug delivery

CHEMICAL COMMUNICATIONS, 50(95), 15105–15108.

By: Y. Lu n, R. Mo n, W. Tai n, W. Sun n, D. Pacardo n, C. Qian n, Q. Shen*, F. Ligler n, Z. Gu n

MeSH headings : Antineoplastic Agents / administration & dosage; Antineoplastic Agents / chemistry; Cell Survival / drug effects; Doxorubicin / administration & dosage; Doxorubicin / chemistry; Drug Carriers / administration & dosage; Drug Carriers / chemistry; Folic Acid / administration & dosage; Folic Acid / chemistry; Glutathione / chemistry; HeLa Cells; Humans; Nanoparticles / administration & dosage; Nanoparticles / chemistry; Oxidation-Reduction; Peptides / chemistry; Polyethylene Glycols / chemistry
TL;DR: Self-folded redox/acid dual-responsive nanocarriers developed for physiologically triggered delivery of anticancer drugs suggest their potential as a promising formulation for tumor-targeted chemotherapy. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Small-Molecule Detection in Thiol-Yne Nanocomposites via Surface-Enhanced Raman Spectroscopy

ANALYTICAL CHEMISTRY, 86(24), 12315–12320.

By: D. Boyd*, F. Bezares*, D. Pacardo n, M. Ukaegbu*, C. Hosten* & F. Ligler n

MeSH headings : Nanocomposites; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman / methods; Sulfhydryl Compounds / chemistry; Surface Properties
TL;DR: Thiol-yne nanocomposite films and fibers fabricated using exposure to long-wave ultraviolet light after the inclusion of gold nanoparticles functionalized with thiophenol indicated that thiol-ynes may be useful as durable film or fiber SERS probes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Automated processing integrated with a microflow cytometer for pathogen detection in clinical matrices

Biosensors and Bioelectronics, 40(1), 10–16.

By: J. Golden*, J. Verbarg*, P. Howell*, L. Shriver-Lake* & F. Ligler*

author keywords: Integrated sample processing; Microfluidics; Flow cytometry; Magnetic microspheres; Multiplexed analysis
MeSH headings : Biosensing Techniques / instrumentation; Equipment Design; Equipment Failure Analysis; Escherichia coli O157 / isolation & purification; Flow Cytometry / instrumentation; Immunomagnetic Separation / instrumentation; Reproducibility of Results; Robotics / instrumentation; Sensitivity and Specificity; Systems Integration
TL;DR: A spinning magnetic trap for automated sample processing was integrated with a microflow cytometer capable of simultaneously detecting multiple targets to provide an automated sample-to-answer diagnosis in 40 min, demonstrating the potential for quantification of pathogen load in buffer and serum. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Crossref
Added: August 28, 2020

2013 journal article

Catch and Release: Integrated System for Multiplexed Detection of Bacteria

Analytical Chemistry, 85(10), 4944–4950.

By: J. Verbarg*, W. Plath*, L. Shriver-Lake*, P. Howell*, J. Erickson*, J. Golden*, F. Ligler*

MeSH headings : Bacteria / cytology; Bacteria / isolation & purification; Flow Cytometry / instrumentation; Humans; Immunomagnetic Separation / instrumentation; Microarray Analysis / instrumentation; Systems Integration
TL;DR: An integrated system with automated immunomagnetic separation and processing of fluidic samples was demonstrated for multiplexed optical detection of bacterial targets and the potential for using the MagTrap to concentrate target from larger volumes of sample prior to the addition of assay reagents was demonstrated. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2013 article

Chemical and biological detection

CHEMICAL SOCIETY REVIEWS, Vol. 42, pp. 8581–8583.

By: C. McEwen*, F. Ligler n & T. Swager*

MeSH headings : Biomedical Engineering; Biosensing Techniques; Biotechnology; Chemistry Techniques, Analytical; Toxins, Biological / analysis
TL;DR: This issue of Chemical Society Reviews highlights a number of exciting developments in chemical/biological sensors from selected leading authorities. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Design and fabrication of uniquely shaped thiol–ene microfibers using a two-stage hydrodynamic focusing design

Lab on a Chip, 13(15), 3105.

By: D. Boyd*, A. Shields*, P. Howell* & F. Ligler*

MeSH headings : Equipment Design; Hydrodynamics; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Polymerization; Polymers / chemistry; Sulfhydryl Compounds / chemistry
TL;DR: This work describes how to fabricate the next generation of fibers predesigned to have a much more complex geometry, as exemplified by the "double anchor" shape, using a two-stage hydrodynamic focusing system. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2013 journal article

Microfabrication: Rapid and Continuous Hydrodynamically Controlled Fabrication of Biohybrid Microfibers (Adv. Funct. Mater. 6/2013)

Advanced Functional Materials, 23(6), 697–697.

By: M. Daniele*, S. North*, J. Naciri*, P. Howell*, S. Foulger*, F. Ligler*, A. Adams*

Source: Crossref
Added: August 28, 2020

2013 article

Nanomaterials in Analytical Chemistry

Ligler, F. S., & White, H. S. (2013, December 3). ANALYTICAL CHEMISTRY, Vol. 85, pp. 11161–11162.

By: F. Ligler n & H. White*

MeSH headings : Chemistry, Analytic / trends; Nanostructures / chemistry
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Simultaneous assay for ten bacteria and toxins in spiked clinical samples using a microflow cytometer

Analytical and Bioanalytical Chemistry, 405(16), 5611–5614.

By: L. Shriver-Lake*, J. Golden*, L. Bracaglia* & F. Ligler*

author keywords: Microflow cytometry; Multiplexed analysis; Bacterial detection; Toxin detection; Biosensor
MeSH headings : Bacteria / isolation & purification; Bacterial Toxins / analysis; Bacterial Toxins / blood; Case-Control Studies; Flow Cytometry / instrumentation; Flow Cytometry / methods; Humans; Immunoassay; Limit of Detection; Random Allocation; Ricin / analysis
TL;DR: The multiplexed assays using the microflow cytometer demonstrated detection limits similar to or better than other reported antibody-based methods for pathogen detection (ELISA, lateral flow, array biosensors) and achieved limits of detection equivalent to those for spiked buffer samples. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2012 journal article

Hydrodynamic Shaping, Polymerization, and Subsequent Modification of Thiol Click Fibers

ACS Applied Materials & Interfaces, 5(1), 114–119.

By: D. Boyd*, A. Shields*, J. Naciri* & F. Ligler*

author keywords: click chemistry; hydrodynamic focusing; microfibers; microfluidics; thiol-ene; thiol-yne
TL;DR: A proof-of-principle experiment demonstrated that biotin attached to thiol groups on the fiber surface could specifically bind a fluorescent protein. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2012 journal article

Hydrodynamic focusing for impedance-based detection of specifically bound microparticles and cells: Implications of fluid dynamics on tunable sensitivity

Sensors and Actuators B: Chemical, 166-167, 386–393.

By: G. Justin*, A. Denisin*, M. Nasir*, L. Shriver-Lake*, J. Golden* & F. Ligler*

author keywords: Microfluidic; Four-electrode impedance; Hydrodynamic focusing; Surface chemistry; Finite element analysis; Fluidic force discrimination
Source: Crossref
Added: August 28, 2020

2012 journal article

Hydrodynamically directed multiscale assembly of shaped polymer fibers

Soft Matter, 8(24), 6656.

By: A. Shields*, C. Spillmann*, J. Naciri*, P. Howell*, A. Thangawng* & F. Ligler*

Source: Crossref
Added: August 28, 2020

2012 journal article

Rapid and Continuous Hydrodynamically Controlled Fabrication of Biohybrid Microfibers

Advanced Functional Materials, 23(6), 698–704.

By: M. Daniele*, S. North*, J. Naciri*, P. Howell*, S. Foulger*, F. Ligler*, A. Adams*

author keywords: cell encapsulation; microfiber extrusion; hydrogel; microfabrication; hydrodynamic focusing
TL;DR: Production of the fibers containing viable bacteria was continuous in contrast to the more typical methods in which cells infiltrated or were attached to prepared fibers, and the biohybrid fibers showed even distribution of encapsulated cells and >90% cell viability. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2012 journal article

Spinning magnetic trap for automated microfluidic assay systems

Lab on a Chip, 12(10), 1793.

By: J. Verbarg*, K. Kamgar-Parsi*, A. Shields*, P. Howell* & F. Ligler*

MeSH headings : Equipment Design; Escherichia coli / isolation & purification; Fluorescent Dyes / chemistry; Immunoassay / methods; Immunoglobulin G / chemistry; Immunomagnetic Separation / methods; Magnets; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microspheres; Models, Theoretical
TL;DR: Results show that sample processing with the MagTrap outperformed the standard manual protocols, improving the detection capability while simultaneously reducing the processing time. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2011 journal article

Erratum to: Hydrodynamic and electrical considerations in the design of a four-electrode impedance-based microfluidic device

Analytical and Bioanalytical Chemistry, 400(9), 3177–3177.

By: G. Justin*, M. Nasir* & F. Ligler*

UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Crossref
Added: August 28, 2020

2011 journal article

Hydrodynamic and electrical considerations in the design of a four-electrode impedance-based microfluidic device

Analytical and Bioanalytical Chemistry, 400(5), 1347–1358.

By: G. Justin*, M. Nasir* & F. Ligler*

author keywords: Hydrodynamic focusing; Impedance spectroscopy; Nyquist plot; Bode impedance; Bode phase; Microfluidics
MeSH headings : Biosensing Techniques / instrumentation; Cytological Techniques / instrumentation; Electric Impedance; Electrodes; Equipment Design; Hydrodynamics; Microfluidic Analytical Techniques / instrumentation
TL;DR: A four-electrode sensor with hydrodynamic focusing to confine that the current was characterized for tunable sensitivity was designed and analyzed for optimization and to resolve issues that would impact sensitivity and reproducibility in subsequent biosensor applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Crossref
Added: August 28, 2020

2011 journal article

Hydrodynamic focusing—a versatile tool

Analytical and Bioanalytical Chemistry, 402(1), 325–335.

By: J. Golden*, G. Justin*, M. Nasir* & F. Ligler*

author keywords: Hydrodynamic focusing; Sheath flow; Microfluidics; Conductivity; Flow focusing; Reynolds number
MeSH headings : Biosensing Techniques / instrumentation; Equipment Design; Flow Cytometry / instrumentation; Hydrodynamics; Microfluidic Analytical Techniques / instrumentation; Microtechnology / instrumentation
TL;DR: The principles for defining the shape of the interface between the focused and focusing fluids are discussed and examples from the lab that use hydrodynamic focusing for impedance-based sensors, flow cytometry, and microfabrication are provided to illustrate the breadth of opportunities for introducing new capabilities into microfluidic systems. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2011 journal article

In SituPhytoplankton Analysis: There’s Plenty of Room at the Bottom

Analytical Chemistry, 84(2), 839–850.

By: J. Erickson*, N. Hashemi*, J. Sullivan, A. Weidemann* & F. Ligler*

MeSH headings : Animals; Biological Assay; Environmental Monitoring; Humans; Marine Biology / methods; Phytoplankton / chemistry; Phytoplankton / drug effects; Phytoplankton / isolation & purification; Water Pollutants, Chemical / analysis
Source: Crossref
Added: January 24, 2021

2011 journal article

Microflow Cytometer for optical analysis of phytoplankton

Biosensors and Bioelectronics, 26(11), 4263–4269.

By: N. Hashemi*, J. Erickson*, J. Golden*, K. Jackson* & F. Ligler*

author keywords: Flow cytometry; Microfluidics; Optical sensing; Hydrodynamic focusing; Phytoplankton
MeSH headings : Diatoms / chemistry; Diatoms / classification; Diatoms / cytology; Equipment Design; Flow Cytometry / instrumentation; Fluorescence; Microfluidic Analytical Techniques / instrumentation; Optical Devices; Optical Phenomena; Phytoplankton / chemistry; Phytoplankton / classification; Phytoplankton / cytology; Scattering, Radiation; Species Specificity; Synechococcus / chemistry
TL;DR: The Microflow Cytometer proved sensitive enough to detect and characterize picoplankton with diameter approximately 1 μm and larger phytoplankton of up to 80 μm in length and the wide range in size discrimination coupled with detection of intrinsic fluorescent pigments suggests that this MicroflowCytometer will be able to distinguish different populations of phy toplankon on unmanned underwater vehicles. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Crossref
Added: August 28, 2020

2011 journal article

Optimization of antibody-conjugated magnetic nanoparticles for target preconcentration and immunoassays

Analytical Biochemistry, 410(1), 124–132.

By: J. Smith*, K. Sapsford*, W. Tan* & F. Ligler*

author keywords: Immunoassay; Magnetic nanoparticles; Total internal reflection fluorescence; Array Biosensor; Protein microarrays
MeSH headings : Analytic Sample Preparation Methods / methods; Animals; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Fluorescent Dyes / chemistry; Immunoassay / instrumentation; Immunoassay / methods; Immunoglobulin G / chemistry; Immunoglobulin G / immunology; Limit of Detection; Magnetics; Nanoparticles / chemistry; Spectrometry, Fluorescence
TL;DR: The results demonstrate the impact of magnetic extraction using the MNPs labeled with fluorescent proteins both for target preconcentration and for subsequent integration into immunoassays performed under flow conditions for enhanced signal generation. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2011 journal article

Optofluidic characterization of marine algae using a microflow cytometer

Biomicrofluidics, 5(3), 032009.

By: N. Hashemi*, J. Erickson*, J. Golden* & F. Ligler*

author keywords: band-pass filters; biological techniques; bioMEMS; bio-optics; fluorescence; light scattering; marine pollution; microchannel flow; microorganisms; molecular biophysics; optical fibres; optical filters; photomultipliers; proteins
TL;DR: The design and fabrication of a Microflow Cytometer for characterization of phytoplankton was demonstrated and the effect of the sheath-to-sample flow-rate ratio on the light scatter and fluorescence of these marine microorganisms was investigated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Crossref
Added: August 28, 2020

2011 journal article

Parameters affecting the shape of a hydrodynamically focused stream

Microfluidics and Nanofluidics, 11(2), 119–128.

By: M. Nasir*, D. Mott*, M. Kennedy*, J. Golden* & F. Ligler*

author keywords: Flow focusing; Angle of confluence; Converging channels; Inertial effects; Laminar flow; Symmetric
Source: Crossref
Added: August 28, 2020

2011 journal article

UV polymerization of hydrodynamically shaped fibers

Lab on a Chip, 11(6), 1157.

By: A. Thangawng*, P. Howell, Jr, C. Spillmann*, J. Naciri* & F. Ligler*

TL;DR: Passive hydrodynamic focusing and UV polymerization of a shaped acrylate stream produced metre-long fibers for structural and mechanical characterization. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2010 journal article

A hard microflow cytometer using groove-generated sheath flow for multiplexed bead and cell assays

Analytical and Bioanalytical Chemistry, 398(5), 1871–1881.

By: A. Thangawng*, J. Kim*, J. Golden*, G. Anderson*, K. Robertson*, V. Low*, F. Ligler*

author keywords: Flow cytometry; Microflow cytometer; Hydrodynamic focusing
MeSH headings : Biological Assay / instrumentation; Biological Assay / methods; Cell Line, Tumor; Flow Cytometry / instrumentation; Flow Cytometry / methods; Humans; Immunoassay; Microspheres; Polymethyl Methacrylate / chemistry
TL;DR: The hard microflow cytometer is a robust device that can operate at high pressures that could withstand pressures greater than 100 psi without leaking. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2010 journal article

Dynamic reversibility of hydrodynamic focusing for recycling sheath fluid

Lab on a Chip, 10(15), 1952.

By: N. Hashemi*, P. Howell, Jr., J. Erickson*, J. Golden* & F. Ligler*

MeSH headings : Flow Cytometry / instrumentation; Flow Cytometry / methods; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microfluidics / instrumentation; Microfluidics / methods; Microspheres; Models, Theoretical; Polystyrenes / chemistry
Source: Crossref
Added: August 28, 2020

2010 journal article

Effect of diffusion on impedance measurements in a hydrodynamic flow focusing sensor

Lab on a Chip, 10(20), 2787.

By: M. Nasir*, D. Price*, L. Shriver-Lake* & F. Ligler*

MeSH headings : Conductometry / instrumentation; Diffusion; Electric Impedance; Equipment Design; Equipment Failure Analysis; Escherichia coli / chemistry; Escherichia coli / physiology; Microfluidic Analytical Techniques / instrumentation; Transducers
TL;DR: The results showed that the diffusion mass transport at the interface of the two streams limited the benefits of increased flow focusing, and increasing flow velocities could be used to offset the diffusion effect. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2010 journal article

Hydrodynamic focusing of conducting fluids for conductivity-based biosensors

Biosensors and Bioelectronics, 25(6), 1363–1369.

By: M. Nasir*, D. Ateya*, D. Burk*, J. Golden* & F. Ligler*

author keywords: Conductivity; Flow-focusing; Biosensor; Reynolds number; Magnetic beads; Hydrodynamic focusing
MeSH headings : Biosensing Techniques / instrumentation; Computer-Aided Design; Electric Conductivity; Electrochemistry / instrumentation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis / instrumentation; Microfluidic Analytical Techniques / instrumentation; Pressure; Solutions / chemistry
TL;DR: Computational simulations conducted in conjunction with experimental flow studies using confocal microscopy show that a flat profile for the focused layer is dependent on the Reynolds number for the chosen flow parameters, and results indicate that a flattening layer is desirable for both increased sensitivity as well as surface-binding efficiency. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Crossref
Added: August 28, 2020

2010 journal article

Iron chelation by cranberry juice and its impact on Escherichia coli growth

BioFactors, 37(2), 121–130.

By: B. Lin*, B. Johnson*, R. Rubin*, A. Malanoski* & F. Ligler*

author keywords: anti-adhesive; antibacterial activities; iron chelation; proanthocyanidins; Vaccinium macrocarpon (cranberry)
MeSH headings : Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Escherichia coli / drug effects; Iron / metabolism; Iron / pharmacology; Iron Chelating Agents / chemistry; Iron Chelating Agents / pharmacology; Plant Extracts / chemistry; Plant Extracts / pharmacology; Proanthocyanidins / metabolism; Vaccinium macrocarpon / chemistry
TL;DR: The gene expression results revealed altered expression of genes associated with iron transport and essential metabolic enzymes as well as with adenosine triphosphate (ATP) synthesis and fumarate hydratase in these cultures, consistent with the strong iron chelating capability of proanthocyanidins, a major constituent of cranberry juice. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2010 journal article

Multiplexed magnetic microsphere immunoassays for detection of pathogens in foods

Sensing and Instrumentation for Food Quality and Safety, 4(2), 73–81.

By: J. Kim*, C. Taitt*, F. Ligler* & G. Anderson*

TL;DR: While the magnetic and fluorescent properties of the Luminex MagPlex microspheres allow for rapid, multiplexed testing for bacterial contamination in typically problematic food matrices, the data demonstrate that achieving desired limits of detection is still a challenge. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2010 journal article

Utilization of microparticles in next-generation assays for microflow cytometers

Analytical and Bioanalytical Chemistry, 398(6), 2373–2382.

By: J. Kim* & F. Ligler*

author keywords: Microparticle; Flow cytometry; Microfluidics; Microflow cytometer; Biosensors
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Calibration; Equipment Design; Flow Cytometry / instrumentation; Flow Cytometry / methods; Light; Magnetics; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microspheres; Particle Size; Scattering, Radiation
TL;DR: This work reviews the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach, and singlexed assays using coded microspheres provide sensitive assays for on-site micro flow cytometers. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 journal article

A simple sheath-flow microfluidic device for micro/nanomanufacturing: fabrication of hydrodynamically shaped polymer fibers

Lab on a Chip, 9(21), 3126.

By: A. Thangawng*, P. Howell Jr, J. Richards*, J. Erickson* & F. Ligler*

MeSH headings : Equipment Design; Microfluidic Analytical Techniques / methods; Microscopy, Electron, Scanning; Nanofibers / chemistry; Polymethyl Methacrylate / chemistry; Surface Properties
TL;DR: A simple sheath flow microfluidic device is used to fabricate polymer micro/nanofibers that have precisely controlled shapes and sizes. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 book review

Common and uncommon sense for sensingIntegrated Analytical Systems, R. Poryrailo (Ed.), Springer (2009)

[Review of Common and uncommon sense for sensing Integrated Analytical Systems, R. Poryrailo (Ed.), Springer (2009), by R. Poryrailo]. Biosensors and Bioelectronics, 25(2), 537.

By: F. Ligler*

Source: Crossref
Added: January 28, 2021

2009 journal article

Multi-wavelength microflow cytometer using groove-generated sheath flow

Lab on a Chip, 9(13), 1942.

By: J. Golden*, J. Kim*, J. Erickson*, L. Hilliard*, P. Howell*, G. Anderson*, M. Nasir*, F. Ligler*

MeSH headings : Animals; Colony Count, Microbial; Equipment Design; Escherichia coli / immunology; Escherichia coli / isolation & purification; Flow Cytometry / instrumentation; Flow Cytometry / methods; Fluorescent Dyes; Immunoglobulin G / immunology; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microspheres; Phycoerythrin / analysis; Phycoerythrin / immunology; Sensitivity and Specificity
TL;DR: A microflow cytometer was developed that ensheathed the sample (core) fluid on all sides and interrogated each particle in the sample stream at four different wavelengths, capable of discriminating microspheres with different amounts of the fluorophores used for coding and detecting the presence of a phycoerythrin antibody complex on the surface of the microsphere. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Crossref
Added: August 28, 2020

2009 journal article

Multiplexed Detection of Bacteria and Toxins Using a Microflow Cytometer

Analytical Chemistry, 81(13), 5426–5432.

By: J. Kim*, G. Anderson*, J. Erickson*, J. Golden*, M. Nasir* & F. Ligler*

MeSH headings : Antibodies, Immobilized / chemistry; Antibodies, Immobilized / immunology; Bacteria / chemistry; Bacteria / isolation & purification; Enterotoxins / analysis; Escherichia coli / chemistry; Flow Cytometry / instrumentation; Flow Cytometry / methods; Fluorescent Dyes / chemistry; Listeria / chemistry; Microfluidics / instrumentation; Microfluidics / methods; Ricin / analysis; Salmonella / chemistry; Signal Processing, Computer-Assisted; Toxins, Biological / analysis
TL;DR: A microfabricated flow cytometer was used to demonstrate multiplexed detection of bacteria and toxins using fluorescent coded microspheres using antibodies bound by the presence of phycoerythrin tracer in a sandwich immunoassay format. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 journal article

Organic Photodiodes for Biosensor Miniaturization

Analytical Chemistry, 81(9), 3455–3461.

By: J. Wojciechowski, L. Shriver-Lake, M. Yamaguchi, E. Füreder, R. Pieler, M. Schamesberger, C. Winder, H. Prall, M. Sonnleitner, F. Ligler*

MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Dose-Response Relationship, Drug; Electrodes; Electronics; Enterotoxins / isolation & purification; Glass / chemistry; Luminescence; Miniaturization; Organic Chemicals / chemistry; Sensitivity and Specificity
TL;DR: The integration of polymer diodes onto a biosensor chip is described to create a disposable device that includes both the detector and the sensing surface coated with immobilized capture antibody. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2009 journal article

Perspective on Optical Biosensors and Integrated Sensor Systems

Analytical Chemistry, 81(2), 519–526.

By: F. Ligler*

MeSH headings : Automation; Biosensing Techniques / instrumentation; Biosensing Techniques / trends; Microfluidics; Nanoparticles; Nanotechnology; Optical Devices
TL;DR: Examples of innovations are identified that will lead to smaller, faster, cheaper optical biosensor systems with capacity to provide effective and actionable information. (via Semantic Scholar)
Source: Crossref
Added: January 28, 2021

2008 journal article

A Parametric Study of Sample Lysis and DNA Purification Techniques for Use in Automated Devices

Analytical Letters, 41(10), 1701–1719.

By: J. Hu*, J. Erickson*, C. Taitt*, B. Lin*, A. Ligler*, K. Blaney*, A. Malanoski*, F. Ligler*

author keywords: automatable; DNA purification; lysis; sample preparation; solid phase extraction
TL;DR: An optimized protocol has been developed for use with both spiked buffers and nasal wash, and all steps of this protocol are suitable for incorporation into a field-portable, automated sample preparation device. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

A combinatorial approach to microfluidic mixing

Journal of Micromechanics and Microengineering, 18(11), 115019.

By: P. Howell, D. Mott, F. Ligler*, J. Golden, C. Kaplan & E. Oran

TL;DR: A new computational approach to the modeling and design of efficient microfluidic mixers is demonstrated, and the mixers created provide far more rapid mixing than previous designs. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Home diagnostics to music

Nature, 456(7219), 178–179.

By: J. Erickson & F. Ligler*

MeSH headings : Biosensing Techniques / economics; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Biosensing Techniques / trends; Compact Disks; Diagnostic Equipment / economics; Diagnostic Equipment / trends; Humans; Microfluidic Analytical Techniques / methods
TL;DR: With the latest technology, you could one day perform bioassays on your home compact-disc player, just like in the doctor's surgery. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Immobilized Proanthocyanidins for the Capture of Bacterial Lipopolysaccharides

Analytical Chemistry, 80(6), 2113–2117.

By: B. Johnson*, J. Delehanty*, B. Lin* & F. Ligler*

MeSH headings : Bacteria / chemistry; Fluorescence; Lipopolysaccharides / chemistry; Proanthocyanidins / analysis
TL;DR: Molar comparison of polymyxin B to proanthocyanidins indicated that the Sepharose immobilized PACs have a binding affinity for LPS similar to that of polymethine B, which is known to bind the lipid A component of LPS. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Impact of cranberry on Escherichia coli cellular surface characteristics

Biochemical and Biophysical Research Communications, 377(3), 992–994.

By: B. Johnson*, B. Lin*, M. Dinderman*, R. Rubin, A. Malanoski* & F. Ligler*

author keywords: Vaccinium macrocarpon; Cranberry; Proanthocyandin; Anti-adhesive; Antibacterial
MeSH headings : Bacterial Adhesion / drug effects; Bacterial Adhesion / physiology; Cell Membrane / drug effects; Cell Membrane / physiology; Cell Membrane / ultrastructure; Escherichia coli / drug effects; Escherichia coli / physiology; Escherichia coli / ultrastructure; Microscopy, Electron, Scanning; Plant Extracts / chemistry; Plant Extracts / pharmacology; Receptors, Immunologic / drug effects; Receptors, Immunologic / physiology; Receptors, Immunologic / ultrastructure; Vaccinium macrocarpon / chemistry
TL;DR: It is shown that the morphology of E. coli is changed when grown in the presence of juice or extract from Vaccinium macrocarpon (cranberry), andGene expression analysis indicates the down regulation of flagellar basal body rod and motor proteins. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

The good, the bad, and the tiny: a review of microflow cytometry

Analytical and Bioanalytical Chemistry, 391(5), 1485–1498.

By: D. Ateya*, J. Erickson*, P. Howell*, L. Hilliard*, J. Golden* & F. Ligler*

author keywords: flow cytometry; microfluidics; fluid focusing; integrated optics; cell sorter
MeSH headings : Biomechanical Phenomena; Biotechnology / instrumentation; Biotechnology / methods; Cell Separation / instrumentation; Cell Separation / methods; Electrophoresis, Microchip / instrumentation; Electrophoresis, Microchip / methods; Equipment Design; Flow Cytometry / instrumentation; Flow Cytometry / methods; Microfluidics / instrumentation; Microfluidics / methods; Microfluidics / trends; Online Systems / instrumentation; Reproducibility of Results; Sensitivity and Specificity
TL;DR: Each of these four areas is discussed in detail in terms of the progress of development, the continuing limitations, and potential future directions for microflow cytometers. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2008 journal article

Two simple and rugged designs for creating microfluidic sheath flow

Lab on a Chip, 8(7), 1097.

By: P. Howell Jr, J. Golden*, L. Hilliard*, J. Erickson*, D. Mott* & F. Ligler*

MeSH headings : Computer Simulation; Equipment Design; Microfluidic Analytical Techniques / instrumentation
TL;DR: A simple design capable of 2-dimensional hydrodynamic focusing is proposed and successfully demonstrated, which eliminates the need to carefully balance the flow of two or more sheath inlets and easily manufactured using current microfabrication techniques. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2007 journal article

Antimicrobial peptides as new recognition molecules for screening challenging species

Sensors and Actuators B: Chemical, 121(1), 150–157.

By: N. Kulagina, K. Shaffer*, F. Ligler* & C. Taitt*

author keywords: antimicrobial peptides; Coxiella burnetti; Brucella melitensis; vaccinia virus; Venezuelan equine encephalitis virus; screening
TL;DR: Direct binding experiments demonstrate the capability of AMPs as recognition molecules for four biothreat agents in biosensor assays and contrast to all bacterial targets tested to date. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Crossref
Added: August 28, 2020

2007 journal article

Automated module for hybridization and staining of commercially produced nucleic acid microarrays

Microfluidics and Nanofluidics, 3(5), 623–628.

By: J. Erickson*, J. Hu*, G. Anderson*, A. Salls*, S. Gray*, J. Golden*, B. Lin*, F. Ligler*

author keywords: microarray; hybridization; automated; diagnostics; portable
TL;DR: A portable module is demonstrated that can perform the hybridization, wash, and stain steps required for processing a nucleic acid microarray; and it performs these steps in a timeline significantly shorter than the standard commercial protocol. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2007 journal article

Binding and Neutralization of Lipopolysaccharides by Plant Proanthocyanidins

Journal of Natural Products, 70(11), 1718–1724.

By: J. Delehanty*, B. Johnson*, T. Hickey*, T. Pons* & F. Ligler*

MeSH headings : Camellia sinensis / chemistry; Humans; Lipopolysaccharides / metabolism; Molecular Structure; NF-kappa B / metabolism; Proanthocyanidins / chemistry; Proanthocyanidins / isolation & purification; Proanthocyanidins / pharmacology; Vaccinium macrocarpon / chemistry; Vitis / chemistry
TL;DR: The binding of PACs from cranberries, tea, and grapes to lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria and the cause of several human illnesses, is analyzed to suggest that they have potential utility in applications that necessitate either the purification and removal of LPS or the in vivo neutralization of L PS. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2007 journal article

Blind Laboratory Trials for Multiple Pathogens in Spiked Food Matrices

Analytical Letters, 40(17), 3219–3231.

By: L. Shriver‐Lake*, J. Erickson*, K. Sapsford*, M. Ngundi*, K. Shaffer*, N. Kulagina, J. Hu*, S. Gray* ...

author keywords: immunosensor; biosensor; salmonella; SEB; pathogen detection
Source: Crossref
Added: August 28, 2020

2007 journal article

Combination of Immunosensor Detection with Viability Testing and Confirmation Using the Polymerase Chain Reaction and Culture

Analytical Chemistry, 79(1), 140–146.

By: B. Johnson-White, B. Lin* & F. Ligler*

MeSH headings : Bacillus subtilis / growth & development; Bacillus subtilis / isolation & purification; Bacillus subtilis / pathogenicity; Bacteria / classification; Bacteria / growth & development; Bacteria / isolation & purification; Bacteria / pathogenicity; Biosensing Techniques; Cell Culture Techniques / methods; Colony Count, Microbial; Electrophoresis / methods; Escherichia coli / growth & development; Escherichia coli / isolation & purification; Escherichia coli / pathogenicity; Food Microbiology; Immunoassay; Polymerase Chain Reaction / methods; Sensitivity and Specificity
TL;DR: This study combines the rapid screening capability of the array biosensor using an immunoassay format with methods for determination of viability, and demonstrates analysis of captured bacteria using the polymerase chain reaction (PCR). (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2007 journal article

Incorporation of18Oxygen into Peptide Mixtures and Analysis with Multi‐Dimensional Chromatography and Mass‐Spectroscopy

Analytical Letters, 40(10), 1864–1878.

By: M. Lassman*, A. Rahbar*, I. Medintz* & F. Ligler*

author keywords: proteomics; mass spectrometry; LC-MS; O-18 labeling; isotope; peptide; chromatography; Saccharomyces cerevisiae
TL;DR: Experimental parameters that affect efficient 18O incorporation with a model bovine serum albumin protein system are investigated and optimized chemistries for labeling the c‐terminus of peptides in a yeast proteome are used. (via Semantic Scholar)
Source: Crossref
Added: January 24, 2021

2007 journal article

Laser ablation of micropores for formation of artificial planar lipid bilayers

Biomedical Microdevices, 9(6), 863–868.

By: T. O’Shaughnessy*, J. Hu*, J. Kulp*, S. Daly* & F. Ligler*

author keywords: lipid bilayer; aperture; laser ablation; ion channel recording
MeSH headings : Biomimetic Materials / chemistry; Biomimetic Materials / radiation effects; Lasers; Lipid Bilayers / chemistry; Lipid Bilayers / radiation effects; Materials Testing; Membranes, Artificial; Molecular Conformation; Polycarboxylate Cement / chemistry; Polycarboxylate Cement / radiation effects; Porosity
TL;DR: The data demonstrate that the apertures produced by laser ablation are highly reproducible and can support both the formation of stable, long-lasting lipid bilayers as well as the recording of ion channels incorporated into the bilayers. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2007 journal article

Target delivery in a microfluidic immunosensor

Biosensors and Bioelectronics, 22(11), 2763–2767.

By: J. Golden*, T. Floyd-Smith*, D. Mott* & F. Ligler*

author keywords: mixer; grooves; microchannels; fluorescence; immunoassay; depletion
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Botulinum Toxins / analysis; Botulinum Toxins / immunology; Drug Delivery Systems / instrumentation; Drug Delivery Systems / methods; Equipment Design; Equipment Failure Analysis; Fluoroimmunoassay / instrumentation; Fluoroimmunoassay / methods; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Reproducibility of Results; Sensitivity and Specificity
TL;DR: Patterned grooves were embossed in the top of microchannels made in PDMS using soft lithography, enhancing delivery of the target from the bulk fluid to the surface and preventing the formation of a depletion layer at the surface. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: August 28, 2020

2006 journal article

982: A Multianalyte Biosensor for Bladder Cancer Diagnostics

Journal of Urology, 175(4S), 316–317.

By: M. Sanchez-Carbayo, L. Shriver-Lake, C. Cordon-Cardo & F. Ligler*

Source: Crossref
Added: August 28, 2020

2006 journal article

A cowpea mosaic virus nanoscaffold for multiplexed antibody conjugation: Application as an immunoassay tracer

Biosensors and Bioelectronics, 21(8), 1668–1673.

By: K. Sapsford*, C. Soto*, A. Blum*, A. Chatterji*, T. Lin*, J. Johnson*, F. Ligler*, B. Ratna*

MeSH headings : Antigens / analysis; Antigens / immunology; Biological Assay / instrumentation; Biological Assay / methods; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Comovirus / immunology; Fluorescent Antibody Technique / instrumentation; Fluorescent Antibody Technique / methods; Immunoassay / instrumentation; Immunoassay / methods; Microarray Analysis / instrumentation; Microarray Analysis / methods; Nanotechnology / instrumentation; Nanotechnology / methods
TL;DR: The ability to label the virus capsid with antibody and up to 60 fluorescent dyes resulted in an improved limit of detection in SEB sandwich immunoassays, when used as a tracer, relative to a mole equivalent of dye-labeled antibody. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Antimicrobial peptide-based array for Escherichia coli and Salmonella screening

Analytica Chimica Acta, 575(1), 9–15.

By: N. Kulagina, K. Shaffer*, G. Anderson*, F. Ligler* & C. Taitt*

author keywords: antimicrobial peptides; salmonella; Escherichia coli; pathogen; screening
Source: Crossref
Added: August 28, 2020

2006 journal article

Detection of Deoxynivalenol in Foods and Indoor Air Using an Array Biosensor

Environmental Science & Technology, 40(7), 2352–2356.

By: M. Ngundi*, S. Qadri*, E. Wallace*, M. Moore*, M. Lassman*, L. Shriver-Lake*, F. Ligler*, C. Taitt*

MeSH headings : Air Pollution, Indoor; Biosensing Techniques; Food Analysis; Spectrometry, Fluorescence; Trichothecenes / analysis
TL;DR: This study has developed and utilized a competitive immunoassay technique to detect DON in various food matrixes and indoor air samples using an array biosensor and demonstrated a simple procedure amenable for on-site use. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Detection of bacterial toxins with monosaccharide arrays

Biosensors and Bioelectronics, 21(7), 1195–1201.

By: M. Ngundi*, C. Taitt*, S. McMurry*, D. Kahne* & F. Ligler*

author keywords: monosaccharides; bacterial cells; cholera toxin; tetanus toxin; array biosensor; fluorescence
MeSH headings : Adsorption; Bacterial Toxins / analysis; Bacterial Toxins / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Coated Materials, Biocompatible / chemistry; Colony Count, Microbial / instrumentation; Colony Count, Microbial / methods; Equipment Design; Equipment Failure Analysis; Monosaccharides / analysis; Monosaccharides / chemistry; Protein Array Analysis / instrumentation; Protein Array Analysis / methods; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence / instrumentation; Spectrometry, Fluorescence / methods
TL;DR: The use of a monosaccharide-based array to detect protein toxins is reported, showing that the binding of the toxins to the carbohydrates is density dependent and semi-selective. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Diagnosis on disc

Nature, 440(7081), 159–160.

By: F. Ligler* & J. Erickson*

MeSH headings : Immunoassay / economics; Immunoassay / instrumentation; Immunoassay / methods; Miniaturization / instrumentation; Miniaturization / methods; Sensitivity and Specificity
TL;DR: A simple, low-cost technique that can identify and quantify many different antigens simultaneously need high-resolution imaging capability and could be music to the authors' ears. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Multiplexed measurement of serum antibodies using an array biosensor

Biosensors and Bioelectronics, 21(10), 1880–1886.

By: M. Moreno-Bondi*, C. Taitt*, L. Shriver-Lake* & F. Ligler*

author keywords: vaccination; antibody production; biosensor; array; immunization; disease exposure; seroconversion
MeSH headings : Animals; Antibodies, Bacterial / blood; Biosensing Techniques; Diphtheria Toxin / blood; Enterotoxins / blood; Goats; Humans; Immunoglobulin G / blood; Protein Array Analysis; Tetanus Toxin / blood
TL;DR: The array biosensor provides the capability for simultaneously measuring titers of antibody against multiple antigens and applications include monitoring for exposure to pathogens and for efficacy of vaccination. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Crossref
Added: August 28, 2020

2006 journal article

Point-of-care biosensor systems for cancer diagnostics/prognostics

Biosensors and Bioelectronics, 21(10), 1932–1942.

By: S. Soper*, K. Brown*, A. Ellington*, B. Frazier*, G. Garcia-Manero*, V. Gau, S. Gutman*, D. Hayes* ...

author keywords: biosensors; cancer; point-of-care
MeSH headings : Biomarkers, Tumor / analysis; Biosensing Techniques / methods; Biosensing Techniques / trends; Humans; Neoplasms / chemistry; Neoplasms / diagnosis; Neoplasms / pathology; Point-of-Care Systems; Prognosis
TL;DR: This review seeks to provide an overview of the current thinking on molecular profiling for diagnosis and prognosis of cancers and provide insight into the current state-of-the-art in the biosensor field and new strategies that must be considered to bring this important technology into the cancer field. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Prevention of Nonspecific Bacterial Cell Adhesion in Immunoassays by Use of Cranberry Juice

Analytical Chemistry, 78(3), 853–857.

By: B. Johnson-White, L. Buquo*, M. Zeinali* & F. Ligler*

MeSH headings : Anti-Bacterial Agents / analysis; Anti-Bacterial Agents / pharmacology; Bacterial Adhesion / drug effects; Beverages; Binding Sites; Biosensing Techniques / methods; Escherichia coli / cytology; Escherichia coli / drug effects; Immunoassay / methods; Plant Extracts / chemistry; Plant Extracts / pharmacology; Sensitivity and Specificity; Time Factors; Vaccinium macrocarpon / chemistry
TL;DR: The ability of cranberry juice to reduce nonspecific adhesion of bacteria to the borosilicate glass microscope slides used in an immunoarray biosensor format is documents. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Rapid detection of foodborne contaminants using an Array Biosensor

Sensors and Actuators B: Chemical, 113(2), 599–607.

By: K. Sapsford*, M. Ngundi*, M. Moore*, M. Lassman*, L. Shriver-Lake*, C. Taitt*, F. Ligler*

author keywords: Array Biosensor; sandwich immunoassay; competitive immunoassay; bacteria; Campylobacter jejuni; mycotoxins; multi-analyte
TL;DR: The Array Biosensor is demonstrated to have the ability to measure both large pathogens, such as the bacteria Campylobacter jejuni, and small toxins, including the mycotoxins ochratoxin A, fumonisin B, aflatoxin B1 and deoxynivalenol. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

Simultaneous determination of kinetic parameters for the binding of cholera toxin to immobilized sialic acid and monoclonal antibody using an array biosensor

Biosensors and Bioelectronics, 22(1), 124–130.

By: M. Ngundi*, C. Taitt* & F. Ligler*

author keywords: cholera toxin; N-Acetylneuraminic acid (Neu5Ac); antibody; kinetics; binding constants; array biosensor
MeSH headings : Antibodies, Monoclonal / metabolism; Biosensing Techniques; Cholera Toxin / immunology; Cholera Toxin / metabolism; Kinetics; Models, Chemical; N-Acetylneuraminic Acid / metabolism; Protein Binding
TL;DR: The affinity constants for binding of cholera toxin to immobilized sialic acid and to anti-CT antibody (as a simultaneous reference) are determined by measuring real-time binding processes using an array biosensor. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2006 journal article

The beadwhacker for maintaining even dispersion of micron-sized beads

Measurement Science and Technology, 18(1), N1–N4.

By: T. Kayagil, J. Richardson, M. Archer, F. Ligler* & J. Erickson

author keywords: mixer; small instruments; magnetic beads; bead-based extraction
Source: Crossref
Added: January 21, 2021

2006 journal article

Toolbox for the design of optimized microfluidic components

Lab on a Chip, 6(4), 540.

By: D. Mott*, P. Howell, Jr., J. Golden*, C. Kaplan*, F. Ligler* & E. Oran*

MeSH headings : Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microfluidics; Models, Theoretical; Viscosity
TL;DR: A computational "toolbox" for the a priori design of optimized microfluidic components consisting of a microchannel under low-Reynolds number, pressure-driven flow, with an arrangement of grooves cut into the top and bottom to generate a tailored cross-channel flow is presented. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

A microfluidic mixer with grooves placed on the top and bottom of the channel

Lab on a Chip, 5(5), 524.

By: P. Howell, Jr., D. Mott*, S. Fertig*, C. Kaplan*, J. Golden*, E. Oran*, F. Ligler*

MeSH headings : Computer Simulation; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microfluidics / instrumentation; Microfluidics / methods; Polymethyl Methacrylate / chemistry; Sensitivity and Specificity; Surface Properties
TL;DR: A new microfluidic mixer is presented consisting of a rectangular channel with grooves placed in the top and bottom, which not only increases the driving force behind the lateral flow, but allows for the formation of advection patterns that cannot be created with structures on the bottom alone. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

A portable automated multianalyte biosensor

Talanta, 65(5), 1078–1085.

By: J. Golden*, C. Taitt*, L. Shriverlake*, Y. Shubin & F. Ligler*

author keywords: array biosensor; multianalyte; fluorescence; portable biosensor
TL;DR: The miniaturization and automation of the array biosensor for portability and on-site use is described, and simultaneous assays were automatically conducted on samples containing both ovalbumin and staphylococcal enterotoxin B. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

A “do-it-yourself” array biosensor

Methods, 37(1), 65–72.

By: J. Golden*, L. Shriver-Lake*, K. Sapsford* & F. Ligler*

author keywords: biosensor; evanescent wave; array; planar waveguide; fluorescence
MeSH headings : Biosensing Techniques / methods; Fluorescence; Immunoassay
TL;DR: This array biosensor, based on a planar waveguide, a modified microscope slide, with a pattern of small sensing regions, can detect many different targets, including proteins, toxins, cells, virus, and explosives with detection limits rivaling those of the ELISA detection system. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

Antimicrobial Peptides for Detection of Bacteria in Biosensor Assays

Analytical Chemistry, 77(19), 6504–6508.

By: N. Kulagina, M. Lassman*, F. Ligler* & C. Taitt*

MeSH headings : Anti-Infective Agents / analysis; Anti-Infective Agents / metabolism; Antimicrobial Cationic Peptides / analysis; Antimicrobial Cationic Peptides / metabolism; Biosensing Techniques / methods; Escherichia coli / isolation & purification; Escherichia coli / metabolism; Salmonella typhimurium / isolation & purification; Salmonella typhimurium / metabolism
TL;DR: The results showed that both specific and nonspecific binding strongly depend on the method used for peptide immobilization, and direct attachment of magainin to the substrate surface not only decreased nonspecial cell binding but also resulted in improved detection limits for both Salmonella and E. coli. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

Array Biosensor for Detection of Ochratoxin A in Cereals and Beverages

Analytical Chemistry, 77(1), 148–154.

By: M. Ngundi*, L. Shriver-Lake*, M. Moore*, M. Lassman*, F. Ligler* & C. Taitt*

MeSH headings : Beverages / analysis; Beverages / microbiology; Biosensing Techniques / methods; Edible Grain / chemistry; Edible Grain / microbiology; Immunoassay; Ochratoxins / analysis; Sensitivity and Specificity
TL;DR: The development of a rapid and highly sensitive array biosensor for the detection and quantitation of ochratoxin A (OTA) using a competitive immunoassay format and measuring the formation of the fluorescent immunocomplex on the waveguide surface. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2005 journal article

Characterization of passive microfluidic mixers fabricated using soft lithography

Microfluidics and Nanofluidics, 2(2), 180–183.

By: T. Floyd-Smith*, J. Golden*, P. Howell* & F. Ligler*

author keywords: microfluidics; micromixer
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: January 21, 2021

2005 journal article

Evanescent wave fluorescence biosensors

Biosensors and Bioelectronics, 20(12), 2470–2487.

By: C. Taitt*, G. Anderson* & F. Ligler*

author keywords: fluorescence; biosensors; evanescent; planar waveguide; fiber optic
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Equipment Design; Equipment Failure Analysis; Fiber Optic Technology / instrumentation; Microfluidics / instrumentation; Microfluidics / methods; Spectrometry, Fluorescence / instrumentation; Spectrometry, Fluorescence / methods
TL;DR: The important patents that have impacted the evolution from laboratory curiosities to fully automated commercial products are summarized and the critical issues that evanescent wave fluorescence biosensors will face in the coming years are addressed. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2004 journal article

A Portable Array Biosensor for Detecting Multiple Analytes in Complex Samples

Microbial Ecology, 47(2), 175–185.

By: C. Taitt*, J. Golden*, Y. Shubin, L. Shriver-Lake*, K. Sapsford*, A. Rasooly*, F. Ligler*

MeSH headings : Antibodies; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Biotinylation; Environmental Microbiology; Environmental Monitoring / methods; Extraterrestrial Environment; Fluorescence; Immunohistochemistry; Spacecraft; Time Factors; Toxins, Biological / analysis
TL;DR: Improvements in the biochemical and engineering aspects of the MAAB have enabled the expansion of the repertoire of analytes detected to include Salmonella typhimurium and Listeria monocytogenes, and also expanded the different sample matrices tested. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Crossref
Added: August 28, 2020

2004 journal article

Design and evaluation of a Dean vortex-based micromixer

Lab on a Chip, 4(6), 663.

By: P. Howell, Jr, D. Mott*, J. Golden* & F. Ligler*

MeSH headings : Centrifugation / instrumentation; Centrifugation / methods; Equipment Design; Equipment Failure Analysis; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Microfluidics / instrumentation; Microfluidics / methods; Miniaturization; Motion
TL;DR: A mixer, based on the Dean vortex, is fabricated and tested in an on-chip format and indicates that increasing the aspect ratio above 1 appeared to provide improved mixing. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2004 journal article

Detection ofCampylobacterandShigellaSpecies in Food Samples Using an Array Biosensor

Analytical Chemistry, 76(2), 433–440.

By: K. Sapsford*, A. Rasooly*, C. Taitt* & F. Ligler*

TL;DR: 25-min, sandwich immunoassays were developed for the detection of Campylobacter and Shigella species in both buffer and a variety of food and beverage samples and the limits of detection and dynamic range were found to vary depending on the sample matrix. (via Semantic Scholar)
Source: Crossref
Added: January 24, 2021

2004 journal article

Fluorescence-based array biosensors for detection of biohazards

Journal of Applied Microbiology, 96(1), 47–58.

By: K. Sapsford*, Y. Shubin, J. Delehanty*, J. Golden*, C. Taitt*, L. Shriver-Lake*, F. Ligler*

MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Environmental Monitoring / instrumentation; Environmental Monitoring / methods; Fluorescence; Hazardous Substances / analysis; Miniaturization / methods
TL;DR: A miniaturized, fully automated, stand-alone array biosensor developed at the Naval Research Laboratory is reported that monitors interactions between binding partners either as the final image or in real-time. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2004 journal article

Real-time analysis of protein adsorption to a variety of thin films

Biosensors and Bioelectronics, 19(9), 1045–1055.

By: K. Sapsford* & F. Ligler*

author keywords: total internal reflection fluorescence (TIRF); thin film; silane surfaces; protein adsorption
MeSH headings : Adsorption; Data Interpretation, Statistical; Glass; Microscopy, Interference; Proteins / pharmacokinetics; Time Factors
TL;DR: The ability of a fluorescence-based array biosensor to screen surfaces for the adsorption of biomolecules in real-time is demonstrated and the interactions of a variety of proteins with a single surface can be monitored. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

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