Works (2)

Updated: August 26th, 2023 21:16

2020 journal article

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

NUCLEIC ACIDS RESEARCH, 48(10), 5624–5638.

By: T. Jacobsen n, F. Ttofali n, C. Liao*, S. Manchalu*, B. Gray* & C. Beisel n

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / classification; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / classification; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism; DNA Cleavage; Endodeoxyribonucleases / chemistry; Endodeoxyribonucleases / classification; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; HEK293 Cells; Humans; Mutation; Phylogeny; Prevotella / enzymology; Protein Biosynthesis; Protein Domains; Transcription, Genetic
TL;DR: Cas12a nucleases therefore can exhibit widely varying properties between otherwise related orthologs, suggesting selective pressure to diversify PAM recognition and supporting expansion of the CRISPR toolbox through ortholog mining and PAM engineering. (via Semantic Scholar)
Source: Web Of Science
Added: October 5, 2020

2019 journal article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis


By: C. Liao n, F. Ttofali n, R. Slotkowski n, S. Denny n, T. Cecil n, R. Leenay n, A. Keung n, C. Beisel*

MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
TL;DR: A modular, one-pot scheme called CRATES to construct CRISPR arrays and array libraries that can facilitate diverse multiplexing applications and help identify factors impacting crRNA biogenesis is presented. (via Semantic Scholar)
Source: Web Of Science
Added: July 22, 2019

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