Fani Ttofali

Works (3)

Updated: April 23rd, 2025 20:41

2024 article

A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

Vento, J. M., Durmusoglu, D., Li, T., Patinios, C., Sullivan, S., Ttofali, F., … Beisel, C. L. (2024, June 26). Molecular Cell, Vol. 84.

By: J. Vento n, D. Durmusoglu n, T. Li n, C. Patinios *, S. Sullivan  n, F. Ttofali n, J. Schaik n, Y. Yu * ...

MeSH headings : Transcription, Genetic; Cell-Free System; DNA Methylation; Protein Biosynthesis; Escherichia coli / genetics; Escherichia coli / metabolism; Transformation, Bacterial; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Plasmids / genetics; Plasmids / metabolism; DNA Modification Methylases / metabolism; DNA Modification Methylases / genetics; Bacterial Proteins / genetics; Bacterial Proteins / metabolism
topics (OpenAlex): Bacterial Genetics and Biotechnology; Bacteriophages and microbial interactions; CRISPR and Genetic Engineering
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Sources: Web Of Science, NC State University Libraries
Added: September 16, 2024

2020 article

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

Jacobsen, T., Ttofali, F., Liao, C., Manchalu, S., Gray, B. N., & Beisel, C. L. (2020, April 7). Nucleic Acids Research.

By: T. Jacobsen n, F. Ttofali n, C. Liao *, S. Manchalu *, B. Gray* & C. Beisel n

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / classification; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / classification; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism; DNA Cleavage; Endodeoxyribonucleases / chemistry; Endodeoxyribonucleases / classification; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; HEK293 Cells; Humans; Mutation; Phylogeny; Prevotella / enzymology; Protein Biosynthesis; Protein Domains; Transcription, Genetic
topics (OpenAlex): CRISPR and Genetic Engineering; Insect symbiosis and bacterial influences; Mosquito-borne diseases and control
TL;DR: Cas12a nucleases therefore can exhibit widely varying properties between otherwise related orthologs, suggesting selective pressure to diversify PAM recognition and supporting expansion of the CRISPR toolbox through ortholog mining and PAM engineering. (via Semantic Scholar)
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Source: Web Of Science
Added: October 5, 2020

2019 article

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

Liao, C., Ttofali, F., Slotkowski, R. A., Denny, S. R., Cecil, T. D., Leenay, R. T., … Beisel, C. L. (2019, July 3). Nature Communications, Vol. 10.

By: C. Liao  n, F. Ttofali n, R. Slotkowski n, S. Denny n, T. Cecil n, R. Leenay n, A. Keung n , C. Beisel*

MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
topics (OpenAlex): CRISPR and Genetic Engineering; Advanced biosensing and bioanalysis techniques; RNA and protein synthesis mechanisms
TL;DR: A modular, one-pot scheme called CRATES to construct CRISPR arrays and array libraries that can facilitate diverse multiplexing applications and help identify factors impacting crRNA biogenesis is presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

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