George C Allen

Works (35)

Updated: April 4th, 2024 22:40

2020 journal article

Comparing DNA replication programs reveals large timing shifts at centromeres of endocycling cells in maize roots

PLOS GENETICS, 16(10).

By: E. Wear n, J. Song, G. Zynda, L. Mickelson-Young n, C. LeBlanc*, T. Lee n, D. Deppong n, G. Allen n ...

MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / genetics; Centromere / drug effects; Centromere / genetics; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication Timing / drug effects; DNA Replication Timing / genetics; DNA, Plant / drug effects; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / pharmacology; Endocytosis / drug effects; Meristem / drug effects; Meristem / genetics; Mitosis / drug effects; Mitosis / genetics; Nucleosomes / drug effects; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / growth & development; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020

2017 journal article

Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (Zea mays) Root Tips

PLANT CELL, 29(9), 2126–2149.

By: E. Wear n, J. Song, G. Zynda, C. LeBlanc*, T. Lee n, L. Mickelson-Young n, L. Concia n, P. Mulvaney n ...

MeSH headings : Base Sequence; Chromosomes, Plant / genetics; DNA Replication Timing / genetics; DNA Transposable Elements / genetics; Genes, Plant; Genomics; Meristem / cytology; Meristem / genetics; Mitosis / genetics; Models, Genetic; S Phase / genetics; Tandem Repeat Sequences / genetics; Time Factors; Transcription, Genetic; Zea mays / cytology; Zea mays / genetics
TL;DR: A novel adaptation of the “Repli-seq” assay for use in intact root tips of maize (Zea mays) that includes several different cell lineages and present whole-genome replication timing profiles from cells in early, mid, and late S phase of the mitotic cell cycle is reported. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

A flow cytometric method for estimating S-phase duration in plants

JOURNAL OF EXPERIMENTAL BOTANY, 67(21), 6077–6087.

By: L. Mickelson-Young n, E. Wear n, P. Mulvaney n, T. Lee n, E. Szymanski n, G. Allen n, L. Hanley-Bowdoin n, W. Thompson n

author keywords: Arabidopsis; barley; EdU; flow cytometry; maize; replication timing; rice; S-phase duration; wheat
MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; DNA, Plant / metabolism; Deoxyuridine / analogs & derivatives; Deoxyuridine / metabolism; Flow Cytometry / methods; G1 Phase / physiology; G2 Phase / physiology; Hordeum / cytology; Hordeum / growth & development; Meristem / cytology; Meristem / growth & development; Oryza / cytology; Oryza / growth & development; S Phase / physiology; Triticum / cytology; Triticum / growth & development; Zea mays / cytology; Zea mays / growth & development
TL;DR: This work estimated S-phase duration for several plant species by following EdU-labeled nuclei from G1 to G2 using bivariate flow cytometry, finding that S- phase duration is relatively consistent over a range of genome sizes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Defining multiple, distinct, and shared spatiotemporal patterns of DNA replication and endoreduplication from 3D image analysis of developing maize (Zea mays L.) root tip nuclei

PLANT MOLECULAR BIOLOGY, 89(4-5), 339–351.

By: H. Bass*, G. Hoffman*, T. Lee n, E. Wear n, S. Joseph*, G. Allen n, L. Hanley-Bowdoin n, W. Thompson n

author keywords: Cell cycle; DNA synthesis; Grass
MeSH headings : Cell Nucleus / genetics; Cell Nucleus / metabolism; DNA Replication / genetics; DNA Replication Timing / genetics; DNA, Plant / biosynthesis; DNA, Plant / genetics; Endoreduplication / genetics; Genes, Plant; Imaging, Three-Dimensional; Meristem / genetics; Meristem / growth & development; Meristem / metabolism; Models, Biological; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development; Zea mays / metabolism
TL;DR: 3D quantitative analysis of DNA replication and endoreduplication in nuclei from pulse-labeled developing maize root tips revealed that maize euchromatin exists as an intermingled mixture of two components distinguished by their condensation state and replication timing. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 review

A maize root tip system to study DNA replication programmes in somatic and endocycling nuclei during plant development

[Review of ]. JOURNAL OF EXPERIMENTAL BOTANY, 65(10), 2747–2756.

By: H. Bass*, E. Wear n, T. Lee n, G. Hoffman*, H. Gumber*, G. Allen n, W. Thompson n, L. Hanley-Bowdoin n

author keywords: DNA replication; EdU; endocycle; flow cytometry; high-resolution microscopy; maize; mitotic cell cycle; root development; S phase
MeSH headings : Base Sequence; Cell Nucleus / genetics; DNA Replication; DNA, Plant / biosynthesis; In Situ Hybridization, Fluorescence; Oligonucleotide Probes; Plant Roots / growth & development; Zea mays / genetics; Zea mays / growth & development
TL;DR: A promising new system that can be used to visualize DNA replication in isolated maize root tip nuclei after in planta pulse labelling with the thymidine analogue, 5-ethynyl-2'-deoxyuridine (EdU). (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 journal article

Effect of various sterilization procedures on the in vitro germination of cotton seeds

PLANT CELL TISSUE AND ORGAN CULTURE, 118(1), 179–185.

By: S. Barampuram n, G. Allen n & S. Krasnyanski n

author keywords: Sterilization; Chlorine gas; Commercial bleach; Hydrogen peroxide; In vitro; Seed germination
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

In Vivo Mapping of Arabidopsis Scaffold/Matrix Attachment Regions Reveals Link to Nucleosome-Disfavoring Poly(dA:dT) Tracts

PLANT CELL, 26(1), 102–120.

By: P. Pascuzzi n, M. Flores-Vergara n, T. Lee n, B. Sosinski n, M. Vaughn, L. Hanley-Bowdoin n, W. Thompson n, G. Allen n

MeSH headings : Arabidopsis / genetics; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Chromatin / metabolism; Gene Expression Regulation, Plant; Matrix Attachment Regions; Nucleosomes / metabolism; Nucleotide Motifs; Poly dA-dT / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription Factors / physiology
TL;DR: This work uses tiling microarrays to map S/MARs on Arabidopsis chromosome 4, found to be spaced more closely than in the large plant and animal genomes studied to date and preferentially enriched in poly(dA:dT) tracts, sequences that resist nucleosome formation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites

PLANT PHYSIOLOGY, 163(1), 30–42.

By: V. Bajwa, X. Wang*, R. Blackburn n, M. Goshe n, S. Mitra*, E. Williams*, G. Bishop*, S. Krasnyanski* ...

MeSH headings : Binding Sites; Kinetics; Solanum lycopersicum / enzymology; Phosphorylation; Plant Proteins / chemistry; Plant Proteins / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Signal Transduction
TL;DR: Liquid chromatography-tandem mass spectrometry was used to identify multiple in vitro phosphorylation sites of the tomato BRI1 and BAK1 cytoplasmic domains and found that tomato TRIP1 is a substrate of both tomato Bri1 andBAK1 kinases in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Overexpression of mannitol dehydrogenase in zonal geranium confers increased resistance to the mannitol secreting fungal pathogen Botrytis cinerea

Plant Cell, Tissue and Organ Culture, 115(3), 367–375.

By: J. Williamson n, A. Desai n, S. Krasnyanski n, F. Ding n, W. Guo n, T. Nguyen n, H. Olson n, J. Dole n, G. Allen n

TL;DR: The results are an important validation of previous work, and support the idea that MTD-overexpression might be used to engineer a broad variety of plants for resistance to mannitol-secreting fungal pathogens like B. cinerea for which specific resistance is lacking. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

A Novel Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Promoter for Expressing Transgenes in the Halotolerant Alga Dunaliella salina

CURRENT MICROBIOLOGY, 64(5), 506–513.

By: Y. Jia*, S. Li*, G. Allen n, S. Feng* & L. Xue*

MeSH headings : Base Sequence; Chlorophyta / drug effects; Chlorophyta / enzymology; Chlorophyta / genetics; Chlorophyta / metabolism; Collagen Type IV / genetics; Collagen Type IV / metabolism; Gene Expression; Glyceraldehyde-3-Phosphate Dehydrogenases / genetics; Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism; Humans; Molecular Sequence Data; Organophosphorus Compounds / pharmacology; Promoter Regions, Genetic; Sodium Chloride / metabolism; Transgenes
TL;DR: A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was cloned and used to drive expressions of the bialaphos resistance (bar) gene and of the N-terminal fragment of human canstatin (Can-N), showing that the bar gene was transcribed by the GAPDH promoter and integrated into the genome of the transformants of D. salina. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State

PLoS Genetics, 6(6), e1000982.

By: T. Lee n, P. Pascuzzi n, S. Settlage n, R. Shultz n, M. Tanurdzic*, P. Rabinowicz*, M. Menges*, P. Zheng* ...

Ed(s): G. Copenhaver

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Chromatin / genetics; Chromosomes, Plant; DNA Replication; Epigenesis, Genetic; Flow Cytometry; Oligonucleotide Array Sequence Analysis; Replicon; S Phase
TL;DR: The similarity of the replication profiles for early and mid S phase cells indicates that replication origin activation in euchromatin is stochastic, and distinct patterns of association of H3K56ac with gene expression and early replication provide evidence that H3k56ac may be associated with initiation zones and replication origins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

Dynamic Localization of the DNA Replication Proteins MCM5 and MCM7 in Plants

PLANT PHYSIOLOGY, 150(2), 658–669.

By: R. Shultz n, T. Lee n, G. Allen n, W. Thompson n & L. Hanley-Bowdoin n

MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Cell Cycle; Cell Cycle Proteins / metabolism; Cell Nucleus / metabolism; Chromatin / metabolism; DNA Replication; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Minichromosome Maintenance Complex Component 7; Multiprotein Complexes / genetics; Multiprotein Complexes / metabolism; Origin Recognition Complex / metabolism; Plant Roots / cytology; Plant Roots / metabolism; Protein Binding; Protein Subunits / genetics; Protein Subunits / metabolism; Protein Transport; Tobacco / cytology; Tobacco / metabolism
TL;DR: It is demonstrated that subunits of the MCM2-7 complex are coordinately expressed during Arabidopsis (Arabidopsis thaliana) development and are abundant in proliferating and endocycling tissues, indicative of a role in DNA replication. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2009 journal article

Rapid shoot regeneration in industrial 'high starch' sweetpotato (Ipomoea batatas L.) genotypes

PLANT CELL TISSUE AND ORGAN CULTURE, 97(1), 109–117.

By: M. Santa-Maria n, K. Pecota n, C. Yencho n, G. Allen n & B. Sosinski n

author keywords: Meristem culture; High dry matter; Organogenesis; Storage roots; Sweet potato; Virus indexing
TL;DR: The results led to the establishment of optimized in vitro regeneration procedures for the novel high-starch sweetpotato (SP) genotypes ‘DM01-158’, ‘FTA94’ , ‘FT489’ and ‘PDM P4’ that are rapid and reliable. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

NATURE PROTOCOLS, 1(5), 2320–2325.

By: G. Allen n, M. Flores-Vergara n, S. Krasnyanski, S. Kumar n & W. Thompson n

MeSH headings : Cetrimonium; Cetrimonium Compounds; DNA, Plant / isolation & purification; Magnoliopsida / chemistry; Plant Leaves / chemistry; Time Factors
TL;DR: The modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 review

Gene targeting in plants: fingers on the move

[Review of ]. TRENDS IN PLANT SCIENCE, 11(4), 159–161.

By: S. Kumar n, G. Allen n & W. Thompson n

MeSH headings : Chromatin Assembly and Disassembly; DNA Repair; Endodeoxyribonucleases / chemistry; Endodeoxyribonucleases / metabolism; Gene Targeting / methods; Genome, Plant; Plants / genetics; Recombination, Genetic; Zinc Fingers / physiology
TL;DR: Zinc-finger endonucleases can be harnessed to stimulate homologous recombination and gene targeting in plants, which represents a major step towards modifying the plant genome more precisely. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 patent

High efficiency gene targeting in plants

Washington, DC: U.S. Patent and Trademark Office.

By: G. Helmer, G. Allen & W. Thompson

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Matrix attachment regions and regulated transcription increase and stabilize transgene expression

PLANT BIOTECHNOLOGY JOURNAL, 3(5), 535–543.

By: R. Abranches n, R. Shultz n, W. Thompson n & G. Allen n

author keywords: PTGS; TGS; RNAi; MARs; transgene expression; induction
TL;DR: Transgene silencing has been shown to be associated with strong promoters, but it is not known whether the propensity for silencing is caused by the level of transcription, or some other property of the promoter, so transgenes with inducible promoters may be less susceptible to silencing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Analysis of trans-silencing interactions using transcriptional silencers of varying strength and targets with and without flanking nuclear matrix attachment regions

TRANSGENIC RESEARCH, 12(3), 305–318.

By: R. Ascenzi n, B. Ulker, J. Todd, D. Sowinski, C. Schimeneck, G. Allen, A. Weissinger, W. Thompson

author keywords: matrix attachment region; tobacco; transcriptional gene silencing; transgene expression; trans-silencing
MeSH headings : Gene Silencing; Inheritance Patterns; Matrix Attachment Regions / genetics; Seedlings / genetics; Sequence Homology; Silencer Elements, Transcriptional; Tobacco / genetics; Transcription, Genetic
TL;DR: The results are consistent with a model in which transcriptional silencing is triggered by transcription of sequences during the early stages of embryo or seedling development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3 ' MAR

TRANSGENIC RESEARCH, 12(1), 3–12.

By: S. Mankin n, G. Allen*, T. Phelan n, S. Spiker n & W. Thompson n

author keywords: gene silencing; matrix attachment regions; transformation; transgene expression
MeSH headings : 3' Untranslated Regions / genetics; DNA Primers / chemistry; Gene Expression Regulation, Plant; Genes, Plant / physiology; Glucuronidase / genetics; Glucuronidase / metabolism; Hot Temperature; Nuclear Matrix / physiology; Plant Leaves / genetics; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Polymerase Chain Reaction; Promoter Regions, Genetic / genetics; Tobacco / genetics; Tobacco / growth & development; Transformation, Genetic; Transgenes / physiology; Up-Regulation
TL;DR: Most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs, which is important for understanding the role of matrix attachment region in transgene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Differential Top10 promoter regulation by six tetracycline analogues in plant cells

JOURNAL OF EXPERIMENTAL BOTANY, 53(376), 1871–1877.

By: J. Love*, G. Allen*, C. Gatz & W. Thompson*

author keywords: tetracycline analogues; tobacco; Top10 promoter activity; tissue culture
MeSH headings : Agrobacterium tumefaciens / genetics; Cell Line; Doxycycline / pharmacology; Gene Expression Regulation, Plant / drug effects; Glucuronidase / genetics; Glucuronidase / metabolism; Minocycline / pharmacology; Oxytetracycline / pharmacology; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Repressor Proteins / genetics; Tetracyclines / chemistry; Tetracyclines / pharmacology; Tobacco / cytology; Tobacco / drug effects; Tobacco / genetics; Transfection
TL;DR: Different tetracycline analogues can therefore be used to regulate the Top10 promoter in plant cells and this property may be exploited in planning an optimum course of transgene regulation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

High-throughput transgene copy number estimation by competitive PCR

PLANT MOLECULAR BIOLOGY REPORTER, 20(3), 265–277.

By: A. Callaway n, R. Abranches n, J. Scroggs n, G. Allen n & W. Thompson n

author keywords: competitive; copy number; high throughput; PCR; quantitative; transgenic
TL;DR: This work applies mathematical modeling to create a rapid, simple, and inexpensive copy number determination method that retains the robustness of competitive PCR. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 patent

Increasing expression of transgenes in plant cells using insulator elements

Washington, DC: U.S. Patent and Trademark Office.

By: W. Thompson, G. Allen & S. Mankin

Source: NC State University Libraries
Added: August 6, 2018

2000 patent

Method for reducing expression variability of transgenes in plant cells

Washington, DC: U.S. Patent and Trademark Office.

By: W. Thompson, G. Allen & S. Mankin

Source: NC State University Libraries
Added: August 6, 2018

2000 review

Use of matrix attachment regions (MARs) to minimize transgene silencing

[Review of ]. PLANT MOLECULAR BIOLOGY, 43(2-3), 361–376.

By: G. Allen*, S. Spiker* & W. Thompson n

author keywords: chromatin structure; gene silencing; MAR; nuclear matrix; nuclear scaffold; SAR
MeSH headings : Animals; DNA / genetics; DNA / metabolism; Gene Expression Regulation, Plant; Gene Silencing; Humans; Nuclear Matrix / metabolism; Nuclear Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Protein Binding; Transgenes / genetics
TL;DR: This review explores current data and presents several plausible models to explain MAR effects on transgene expression and results in higher and more stable expression in transgenic plants or cell lines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants

PLANT JOURNAL, 18(3), 253–263.

By: B. Ulker, G. Allen*, W. Thompson*, S. Spiker* & A. Weissinger*

TL;DR: In primary transgenic plants, the average uidA expression in plants with MARs was twofold greater than in control plants without MARs, but there was no effect on variation of expression. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

Characterization of randomly-obtained matrix attachment regions (MARs) from higher plants

BIOCHEMISTRY, 38(39), 12795–12804.

By: S. Michalowski n, G. Allen n, G. Hall n, W. Thompson n & S. Spiker n

MeSH headings : Base Sequence; Cloning, Molecular; DNA, Plant; Molecular Sequence Data; Nuclear Matrix / metabolism; Plants / metabolism
TL;DR: Binding strength is a function of both numbers of binding sites and affinity for the sites, and a newly identified motif, the "90%AT Box," correlates better with binding strength than any of the previously identified motifs investigated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny

PLANT JOURNAL, 18(3), 233–242.

By: P. Vain*, B. Worland*, A. Kohli*, J. Snape*, P. Christou*, G. Allen n, W. Thompson n

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 journal article

Ferredoxin-1 mRNA is destabilized by changes in photosynthetic electron transport

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 95(15), 9009–9013.

By: M. Petracek n, L. Dickey n, T. Nguyen n, C. Gatz n, D. Sowinski n, G. Allen n, W. Thompson n

MeSH headings : Diuron / pharmacology; Electron Transport; Ferredoxins / genetics; Half-Life; Histones / genetics; Photosynthesis / drug effects; Plants, Genetically Modified / genetics; Plants, Toxic; Promoter Regions, Genetic; RNA, Messenger / metabolism; Tetracycline / pharmacology; Tobacco / genetics
TL;DR: The tetracycline repressible Top10 promoter system is used to specifically shut off transcription of the Fed-1 transgene, and data indicate that cessation of photosynthesis, either by darkness or DCMU results in a destabilization of theFed-1 mRNA. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 patent

Method of increasing expression for foreign genes in plant cells

Washington, DC: U.S. Patent and Trademark Office.

George Allen; Steven Spiker; William Thompson

Source: NC State University Libraries
Added: August 6, 2018

1998 patent

Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells

Washington, DC: U.S. Patent and Trademark Office.

William Thompson; George Allen; Steven Spiker

Source: NC State University Libraries
Added: August 6, 2018

1998 chapter

The dependence of Fed-1 light regulation on translation

In J. Bailey-Serres & D. R. Gallie (Eds.), A look beyond transcription: Mechanisms determining mRNAstability and translation in plants (pp. 96–101). Washington, DC: American Society of Plant Physiologists.

By: M. Petracek, L. Dickey, E. Hansen, D. Sowinski, T. Nguyen, G. Allen, G. Thompson

Ed(s): . J. Bailey-Serres & D. Gallie

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Introduction of a plant intron into the luciferase gene of Photinus pyralis

Plant Molecular Biology Reporter, 15(2), 186–196.

By: S. Mankin n, G. Allen n & W. Thompson n

TL;DR: A new luciferase reporter gene,lucINT, is described, allowing earlier detection of gene expression in the presence of Agrobacterium during transformations in tobacco leaf discs. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1996 journal article

High-Level Transgene Expression in Plant Cells: Effects of a Strong Scaffold Attachment Region from Tobacco

The Plant Cell, 8(5), 899.

By: G. Allen*, G. Hall, S. Michalowski, W. Newman, S. Spiker, A. Weissinger, W. Thompson*

Sources: NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018

1993 journal article

Scaffold attachment regions increase reporter gene expresssion in stably transformed plant cells

Plant Cell, 5(6), 603–613.

By: G. Allen*, G. Hall, L. Childs, A. Weissinger, S. Spiker & W. Thompson*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1991 journal article

Oxygen uptake and hydrogen-stimulated nitrogenase activity from Azorhizobium caulinodans ORS571 grown in a succinate-limited chemostat

Applied and Environmental Microbiology, 57(11), 3220.

By: G. Allen, D. Grimm & G. Elkan

Source: NC State University Libraries
Added: August 6, 2018

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