Gavin Williams

https://orcid.org/0000-0003-2172-9063

Synthetic biology, Natural products, Protein engineering, Metabolic engineering, Drug development

Works (55)

Updated: October 12th, 2023 05:01
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2023 article

Current State-of-the-Art Toward Chemoenzymatic Synthesis of Polyketide Natural Products

Paulsel, T. Q., & Williams, G. J. (2023, September 15). CHEMBIOCHEM, Vol. 9.

By: T. Paulsel n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: bioorganic chemistry; biosynthesis; enzymes; polyketides; synthetic biology
Sources: Web Of Science, ORCID
Added: October 10, 2023

2022 journal article

Targeted enzyme modifications enable regioselective biosynthesis of fluorinated polyketides

CHEM CATALYSIS, 2(10), 2440–2443.

By: S. Welch n, J. Cossin n, T. Paulsel n & G. Williams n 

co-author countries: United States of America 🇺🇸
Sources: Web Of Science, ORCID
Added: January 17, 2023

2021 journal article

Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase

NATURE COMMUNICATIONS, 12(1).

By: E. Kalkreuter  n, K. Bingham n, A. Keeler  n, A. Lowell *, J. Schmidt*, D. Sherman *, G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Acyltransferases / metabolism; Catalytic Domain; Malonyl Coenzyme A; Molecular Dynamics Simulation; Mutagenesis; Polyketide Synthases / chemistry; Polyketide Synthases / genetics; Polyketide Synthases / metabolism; Polyketides; Protein Engineering; Secondary Metabolism; Substrate Specificity
Sources: ORCID, Web Of Science
Added: April 14, 2021

2021 journal article

Development of Genetically Encoded Biosensors for Reporting the Methyltransferase-Dependent Biosynthesis of Semisynthetic Macrolide Antibiotics

ACS SYNTHETIC BIOLOGY, 10(10), 2520–2531.

By: Y. Li  n, M. Reed *, H. Wright*, T. Cropp * & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: macrolide; polyketide; biosensor; transcription factor; synthetic biology
MeSH headings : Anti-Bacterial Agents / biosynthesis; Anti-Bacterial Agents / chemistry; Biosensing Techniques / methods; Macrolides / chemistry; Macrolides / metabolism; Methyltransferases / metabolism; Molecular Structure; Mutagenesis
Sources: ORCID, Web Of Science
Added: September 22, 2021

2021 review

Protein engineering for natural product biosynthesis and synthetic biology applications

[Review of ]. PROTEIN ENGINEERING DESIGN & SELECTION, 34.

By: M. Calzini n, A. Malico n, M. Mitchler n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: biosynthesis; natural products; peptides; polyketides; synthetic biology; terpenes
MeSH headings : Biocatalysis; Biological Products; Peptides / metabolism; Protein Engineering; Synthetic Biology
Sources: Web Of Science, ORCID
Added: September 27, 2021

2021 review

Transcription factor-based biosensors: a molecular-guided approach for natural product engineering

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 69, 172–181.

By: M. Mitchler n, J. Garcia n, N. Montero n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Biological Products; Biosensing Techniques; Biosynthetic Pathways; High-Throughput Screening Assays; Metabolic Engineering; Transcription Factors / genetics
Sources: Web Of Science, ORCID
Added: July 26, 2021

2020 journal article

An artificial pathway for polyketide biosynthesis

Nature Catalysis, 3(7), 536–538.

By: A. Gayen n, L. Nichols n & G. Williams n 

co-author countries: United States of America 🇺🇸
Sources: Web Of Science, ORCID, Crossref
Added: August 3, 2020

2020 journal article

Cheminformatics Analysis and Modeling with MacrolactoneDB

Scientific Reports, 10(1).

By: P. Zin n , G. Williams n  & S. Ekins  n

co-author countries: United States of America 🇺🇸
MeSH headings : Biological Products / chemistry; Cheminformatics; Databases, Chemical; Machine Learning; Macrolides / chemistry; Models, Chemical; Quantitative Structure-Activity Relationship; Software
Sources: Web Of Science, ORCID, Crossref
Added: September 7, 2020

2020 article

Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase

Kalkreuter, E., Bingham, K. S., Keeler, A. M., Lowell, A. N., Schmidt, J. J., Sherman, D. H., & Williams, G. J. (2020, April 25). (Vol. 4). Vol. 4.

By: E. Kalkreuter  n, K. Bingham n, A. Keeler  n, A. Lowell *, J. Schmidt*, D. Sherman *, G. Williams n 

co-author countries: United States of America 🇺🇸
Source: ORCID
Added: December 15, 2020

2020 journal article

SIME: synthetic insight-based macrolide enumerator to generate the V1B library of 1 billion macrolides

Journal of Cheminformatics, 12(1).

By: P. Zin n , G. Williams n  & D. Fourches n

co-author countries: United States of America 🇺🇸
author keywords: Macrolides; PKS enumerator; In silico chemical library software; Polyketides
Sources: Web Of Science, ORCID, Crossref
Added: April 27, 2020

2020 journal article

Synthetic biology enabling access to designer polyketides

Current Opinion in Chemical Biology, 58, 45–53.

By: A. Malico n, L. Nichols n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: Polyketides; Synthetic biology; Metabolic engineering; Enzyme engineering; Natural products
MeSH headings : Genetic Engineering; Multigene Family; Polyketides / chemistry; Polyketides / metabolism; Synthetic Biology / methods
Sources: Crossref, ORCID, Web Of Science
Added: December 22, 2020

2020 article

Synthetic biology, combinatorial biosynthesis, and chemo-enzymatic synthesis of isoprenoids (September, 10.1007/s10295-020-02306-3, 2020)

Malico, A. A., Calzini, M. A., Gayen, A. K., & Williams, G. J. (2020, December). JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, Vol. 47, pp. 1181–1181.

By: A. Malico n, M. Calzini n, A. Gayen n & G. Williams n 

co-author countries: United States of America 🇺🇸
Sources: Web Of Science, ORCID
Added: November 30, 2020

2020 journal article

Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids

Journal of Industrial Microbiology & Biotechnology, 47(9-10), 675–702.

By: A. Malico n, M. Calzini n, A. Gayen n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: Isoprenoids; Terpenes; Synthetic biology; Combinatorial biosynthesis; Hemiterpenes
MeSH headings : Biological Products; Hemiterpenes; Synthetic Biology; Terpenes / chemistry
Sources: Web Of Science, ORCID, Crossref
Added: September 21, 2020

2019 journal article

An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism

ACS Synthetic Biology, 8(2), 232–238.

By: S. Lund n, R. Hall n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: isoprenoids; terpenoids; hemiterpene; kinase; lycopene; prenyltransferase
MeSH headings : Biosynthetic Pathways; Dimethylallyltranstransferase / metabolism; Hemiterpenes / metabolism; Lycopene / metabolism; Metabolic Engineering; Terpenes / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: March 11, 2019

2019 journal article

Cover Feature: Probing the Substrate Promiscuity of Isopentenyl Phosphate Kinase as a Platform for Hemiterpene Analogue Production (ChemBioChem 17/2019)

ChemBioChem, 8.

By: S. Lund n, T. Courtney  n & G. Williams n 

co-author countries: United States of America 🇺🇸
Source: ORCID
Added: December 15, 2020

2019 journal article

Development of a Genetically Encoded Biosensor for Detection of Polyketide Synthase Extender Units in Escherichia coli

ACS Synthetic Biology, 8(6), 1391–1400.

By: E. Kalkreuter  n, A. Keeler  n, A. Malico n, K. Bingham n, A. Gayen n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Biosensing Techniques / methods; Escherichia coli / genetics; Escherichia coli Proteins / genetics; Escherichia coli Proteins / isolation & purification; Escherichia coli Proteins / metabolism; Malonyl Coenzyme A / analysis; Malonyl Coenzyme A / metabolism; Metabolic Networks and Pathways; Polyketide Synthases / metabolism; Polyketides / metabolism; Protein Engineering / methods; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Synthetic Biology; Transcription Factors / genetics; Transcription Factors / isolation & purification; Transcription Factors / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: July 15, 2019

2019 journal article

Engineering the Substrate Specificity of a Modular Polyketide Synthase for Installation of Consecutive Non-Natural Extender Units

Journal of the American Chemical Society, 141(5), 1961–1969.

By: E. Kalkreuter  n, J. CroweTipton n, A. Lowell *, D. Sherman * & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Molecular Structure; Polyketide Synthases / chemistry; Polyketide Synthases / metabolism; Protein Engineering; Substrate Specificity
Sources: Web Of Science, ORCID, Crossref
Added: February 25, 2019

2019 journal article

Probing the Substrate Promiscuity of Isopentenyl Phosphate Kinase as a Platform for Hemiterpene Analogue Production

ChemBioChem, 20(17), 2217–2221.

By: S. Lund n, T. Courtney  n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: hemiterpenes; kinases; phosphorylation; synthetic biology; terpenoids
MeSH headings : Alcohols; Diphosphates / metabolism; Hemiterpenes / chemical synthesis; Phosphates / metabolism; Protein Kinases / metabolism; Substrate Specificity; Synthetic Biology / methods; Terpenes / chemical synthesis; Thermoplasma / enzymology
Sources: Web Of Science, ORCID, Crossref
Added: August 12, 2019

2019 article

Probing the Substrate Promiscuity of Isopentenyl Phosphate Kinase as a Platform for Hemiterpene Analogue Production

Lund, S., Courtney, T., & Williams, G. (2019, February 26). (Vol. 2). Vol. 2.

By: S. Lund, T. Courtney & G. Williams* 

Source: ORCID
Added: October 25, 2021

2018 journal article

Cheminformatics-based enumeration and analysis of large libraries of macrolide scaffolds

Journal of Cheminformatics, 10(1).

By: P. Zin n , G. Williams n  & D. Fourches n

co-author countries: United States of America 🇺🇸
Sources: Web Of Science, ORCID, Crossref
Added: December 3, 2018

2018 journal article

Development of Transcription Factor-Based Designer Macrolide Biosensors for Metabolic Engineering and Synthetic Biology

ACS Synthetic Biology, 7(1), 227–239.

By: C. Kasey n, M. Zerrad n, Y. Li  n, T. Cropp * & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Actinobacteria / drug effects; Actinobacteria / growth & development; Binding Sites; Biosensing Techniques; Directed Molecular Evolution; Erythromycin / biosynthesis; Erythromycin / pharmacology; Ligands; Macrolides / metabolism; Metabolic Engineering; Molecular Dynamics Simulation; Mutagenesis; Protein Structure, Tertiary; Synthetic Biology / methods; Transcription Factors / chemistry; Transcription Factors / genetics; Transcription Factors / metabolism
Sources: Crossref, ORCID
Added: November 29, 2020

2018 journal article

Engineering enzymatic assembly lines for the production of new antimicrobials

Current Opinion in Microbiology, 45, 140–148.

By: E. Kalkreuter  n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Anti-Infective Agents / chemical synthesis; Anti-Infective Agents / chemistry; Anti-Infective Agents / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Metabolic Engineering; Peptide Synthases / chemistry; Peptide Synthases / genetics; Peptide Synthases / metabolism; Polyketide Synthases / chemistry; Polyketide Synthases / genetics; Polyketide Synthases / metabolism; Synthetic Biology
Sources: Web Of Science, ORCID, Crossref
Added: January 21, 2019

2018 journal article

Extender Unit Promiscuity and Orthogonal Protein Interactions of an Aminomalonyl-ACP Utilizing Trans-Acyltransferase from Zwittermicin Biosynthesis

ACS Chemical Biology, 13(12), 3361–3373.

By: S. Carpenter n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acyl Carrier Protein / chemistry; Acyl Carrier Protein / genetics; Acyl Carrier Protein / metabolism; Acyltransferases / chemistry; Acyltransferases / genetics; Acyltransferases / metabolism; Bacillus thuringiensis / enzymology; Bacillus thuringiensis / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Catalytic Domain; Coenzyme A Ligases / metabolism; Escherichia coli / genetics; Kinetics; Molecular Docking Simulation; Molecular Dynamics Simulation; Polyketide Synthases / chemistry; Polyketide Synthases / genetics; Polyketide Synthases / metabolism; Protein Binding; Protein Engineering / methods; Rhizobium / enzymology; Substrate Specificity; Transferases (Other Substituted Phosphate Groups) / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: January 14, 2019

2017 journal article

Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI

Analytical and Bioanalytical Chemistry, 410(3), 953–962.

By: M. Nazari  n, A. Malico n, M. Ekelöf n, S. Lund n, G. Williams n  & D. Muddiman n 

co-author countries: United States of America 🇺🇸
author keywords: Terpenes; IR-MALDESI; SESI; Direct analysis; Biological buffers; Q Exactive Plus
MeSH headings : Alkenes / analysis; Buffers; Infrared Rays; Ions / analysis; Monocyclic Sesquiterpenes; Sesquiterpenes / analysis; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Terpenes / analysis
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2017 journal article

Inversion of Extender Unit Selectivity in the Erythromycin Polyketide Synthase by Acyltransferase Domain Engineering

ACS Chemical Biology, 12(1), 114–123.

By: I. Koryakina n, C. Kasey n, J. McArthur *, A. Lowell *, J. Chemler*, S. Li *, D. Hansen *, D. Sherman *, G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Acyltransferases / metabolism; Erythromycin / chemistry; Erythromycin / metabolism; Macrolides / chemistry; Macrolides / metabolism; Mutagenesis, Site-Directed / methods; Point Mutation; Polyketide Synthases / chemistry; Polyketide Synthases / genetics; Polyketide Synthases / metabolism; Polyketides / chemistry; Polyketides / metabolism; Protein Domains; Saccharopolyspora / enzymology; Saccharopolyspora / genetics; Saccharopolyspora / metabolism; Substrate Specificity
Sources: ORCID, Crossref
Added: December 15, 2020

2017 journal article

Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

ACS Synthetic Biology, 6(3), 421–427.

By: E. Musiol-Kroll *, F. Zubeil*, T. Schafhauser*, T. Härtner*, A. Kulik*, J. McArthur n, I. Koryakina n, W. Wohlleben * ...

co-author countries: Germany 🇩🇪 Denmark 🇩🇰 United States of America 🇺🇸
author keywords: antibiotic; kirromycin; polyketide synthase; trans-acyltransferase; engineering click chemistry
MeSH headings : Acyltransferases / metabolism; Anti-Bacterial Agents / metabolism; Bacteria / metabolism; Bacterial Proteins / metabolism; Polyketide Synthases / metabolism; Polyketides / metabolism; Pyridones / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Harnessing natural product assembly lines: structure, promiscuity, and engineering

Journal of Industrial Microbiology & Biotechnology, 43(2-3), 371–387.

By: C. Ladner n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: Combinatorial biosynthesis; Synthetic biology; Enzyme engineering; Polyketide synthases; Non-ribosomal peptide synthases
MeSH headings : Biological Products / chemistry; Biological Products / metabolism; Biosynthetic Pathways / genetics; Metabolic Engineering; Peptides / metabolism; Polyketides / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Harnessing the promiscuity of natural product biosynthesis: A platform for engineering pathways with new specificities

Planta Medica, 81(11).

By: G. Williams n 

co-author countries: United States of America 🇺🇸
Sources: Crossref, ORCID
Added: December 22, 2020

2014 journal article

Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis

Rapid Communications in Mass Spectrometry, 28(23), 2511–2522.

By: S. Randall n, I. Koryakina n, G. Williams n  & D. Muddiman n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acyltransferases / metabolism; Chromatography, Liquid / methods; Filtration; Hydrophobic and Hydrophilic Interactions; Mass Spectrometry / methods; Oligopeptides / chemistry; Oligopeptides / metabolism; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Polyketides / chemistry; Polyketides / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Intracellular Light-Activation of Riboswitch Activity

ChemBioChem, 15(9), 1346–1351.

By: S. Walsh n, L. Gardner n, A. Deiters n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: chemical biology; photocontrol; riboswitches; RNA; synthetic biology
MeSH headings : Cell Membrane Permeability; Gene Expression Regulation / drug effects; Gene Expression Regulation / radiation effects; Intracellular Space / drug effects; Intracellular Space / genetics; Intracellular Space / radiation effects; Ligands; Molecular Structure; Riboswitch / drug effects; Riboswitch / genetics; Riboswitch / radiation effects; Structure-Activity Relationship; Theophylline / chemistry; Theophylline / pharmacology; Ultraviolet Rays
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Mapping a Ketosynthase:Acyl Carrier Protein Binding Interface via Unnatural Amino Acid-Mediated Photo-Cross-Linking

Biochemistry, 53(48), 7494–7502.

By: Z. Ye n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acetyltransferases / chemistry; Acetyltransferases / genetics; Acetyltransferases / metabolism; Acyl Carrier Protein / chemistry; Acyl Carrier Protein / genetics; Acyl Carrier Protein / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Conserved Sequence; Cross-Linking Reagents; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Fatty Acid Synthase, Type II / chemistry; Fatty Acid Synthase, Type II / genetics; Fatty Acid Synthase, Type II / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Open Reading Frames; Photochemical Processes; Protein Interaction Domains and Motifs; Saccharopolyspora / genetics; Saccharopolyspora / metabolism; Streptomyces coelicolor / genetics; Streptomyces coelicolor / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Reprogramming Acyl Carrier Protein Interactions of an Acyl-CoA Promiscuous trans-Acyltransferase

Chemistry & Biology, 21(5), 636–646.

By: Z. Ye n, E. Musiol*, T. Weber * & G. Williams n 

co-author countries: Germany 🇩🇪 United States of America 🇺🇸
MeSH headings : Acyl Carrier Protein / metabolism; Acyl Coenzyme A / metabolism; Acyltransferases / metabolism; Molecular Structure; Pyridones / metabolism
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2014 conference paper

Synthetic Biology Approaches For Combinatorial Biosynthesis Of Polyketide Natural Products.

Abstracts of Papers for the American Chemical Society. Presented at the 247th National Spring Meeting of the American Chemical Society, Dallas, Texas, USA.

By: I. Koryakina & G. Williams 

Event: 247th National Spring Meeting of the American Chemical Society at Dallas, Texas, USA on March 16, 2014

Source: NC State University Libraries
Added: December 30, 2020

2013 journal article

Engineering polyketide synthases and nonribosomal peptide synthetases

Current Opinion in Structural Biology, 23(4), 603–612.

By: G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Antineoplastic Agents / chemical synthesis; Antineoplastic Agents / metabolism; Gene Expression; Mutagenesis, Site-Directed / methods; Peptide Biosynthesis, Nucleic Acid-Independent; Peptide Synthases / metabolism; Polyketide Synthases / metabolism; Polyketides / chemical synthesis; Protein Engineering / methods
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2013 journal article

Promiscuity of a modular polyketide synthase towards natural and non-natural extender units

Organic & Biomolecular Chemistry, 11(27), 4449.

By: I. Koryakina n, J. McArthur n, M. Draelos n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acyl Coenzyme A / chemistry; Acyl Coenzyme A / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Coenzyme A Ligases / chemistry; Coenzyme A Ligases / metabolism; Polyketide Synthases / chemistry; Polyketide Synthases / metabolism; Protein Structure, Tertiary; Rhizobium / chemistry; Rhizobium / enzymology; Rhizobium / metabolism; Substrate Specificity
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2013 chapter

Reprogramming the Biosynthesis of Natural Products by Directed Evolution

In A. Kantardjieff, P. Asuri, J. L. Coffman, & K. Jayapal (Eds.), Developments in Biotechnology and Bioprocessing (pp. 147–163).

By: G. Williams n , I. Koryakina n, J. McArthur  n, M. Draelos  n, S. Randal n & D. Muddimanl n

co-author countries: United States of America 🇺🇸

Ed(s): A. Kantardjieff, P. Asuri, J. Coffman & K. Jayapal

Sources: Crossref, ORCID
Added: December 22, 2020

2012 chapter

Engineering Glycosyltransferases

In S. Lutz & U. T. Bornscheuer (Eds.), The Protein Engineering Handbook (Vol. 3, pp. 303–326). Weinheim: Wiley-HCH.

By: J. McArthur & G. Williams 

Ed(s): S. Lutz & U. Bornscheuer

Source: NC State University Libraries
Added: December 30, 2020

2012 journal article

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

ACS Chemical Biology, 8(1), 200–208.

By: I. Koryakina*, J. McArthur*, S. Randall, M. Draelos*, E. Musiol*, D. Muddiman* , T. Weber *, G. Williams* 

co-author countries: Germany 🇩🇪
MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Chromatography, Liquid; Coenzyme A Ligases / chemistry; Coenzyme A Ligases / genetics; Crystallography, X-Ray; Genetic Variation; Models, Molecular; Substrate Specificity
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2012 conference paper

Reprogramming the biosynthesis of natural products by directed evolution.

Abstracts of Papers of the American Chemical Society. Presented at the 243rd National Spring Meeting of the American Chemical Society, San Diego, CA, USA.

By: I. Koryakina, Z. Ye, J. McArthur & G. Williams 

Event: 243rd National Spring Meeting of the American Chemical Society at San Diego, CA, USA on March 25, 2012

Source: NC State University Libraries
Added: December 30, 2020

2011 journal article

A High-Throughput Screen for Directed Evolution of the Natural Product Sulfotransferase LipB

Journal of Biomolecular Screening, 16(8), 845–851.

By: I. Koryakina n, J. Neville  n, K. Nonaka, S. Van Lanen * & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: chemistry; medicinal; organic; synthetic; and combinatorial; enzyme assays or enzyme kinetics; anti-infective drugs; natural products screening
MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Acyltransferases / metabolism; Aminoglycosides / metabolism; Biocatalysis; Biological Products / chemistry; Biological Products / metabolism; Biotransformation; Cloning, Molecular; Colorimetry; Escherichia coli / chemistry; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Gene Library; High-Throughput Screening Assays; Nitrobenzenes / analysis; Nitrobenzenes / metabolism; Plasmids; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Small Molecule Libraries / analysis; Small Molecule Libraries / chemistry; Substrate Specificity
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2011 journal article

A high-throughput screen for directed evolution of aminocoumarin amide synthetases

Analytical Biochemistry, 419(1), 61–66.

By: N. Parajuli* & G. Williams* 

author keywords: Directed evolution; Aminocoumarin; High-throughput screening; Natural products
MeSH headings : Acylation; Amide Synthases / metabolism; Aminocoumarins / chemistry; Aminocoumarins / metabolism; Chromatography, High Pressure Liquid; Cloning, Molecular; Directed Molecular Evolution / methods; Enzyme Activation; Escherichia coli / genetics; Escherichia coli / metabolism; Fluorescence; Fluorometry / methods; High-Throughput Screening Assays / methods; Molecular Structure; Novobiocin / analogs & derivatives; Novobiocin / chemistry; Novobiocin / metabolism; Plasmids / genetics; Plasmids / metabolism; Reproducibility of Results; Substrate Specificity
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2011 journal article

A photocrosslinking assay for reporting protein interactions in polyketide and fatty acid synthases

Molecular BioSystems, 7(11), 3152.

By: Z. Ye n, M. Bair n, H. Desai n & G. Williams n 

co-author countries: United States of America 🇺🇸
MeSH headings : Acyl Carrier Protein / chemistry; Acyl Carrier Protein / metabolism; Codon, Terminator; Cross-Linking Reagents / chemistry; Cross-Linking Reagents / metabolism; Fatty Acid Synthases / chemistry; Fatty Acid Synthases / metabolism; Kinetics; Mutagenesis, Site-Directed; Polyketide Synthases / chemistry; Polyketide Synthases / metabolism; Polyketides / metabolism; Protein Conformation
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2011 journal article

Inside Cover: Mutant Malonyl-CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation (ChemBioChem 15/2011)

ChemBioChem, 12(15), 2230–2230.

By: I. Koryakina n & G. Williams n 

co-author countries: United States of America 🇺🇸
Sources: ORCID, Crossref
Added: December 15, 2020

2011 journal article

Mutant Malonyl-CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation

ChemBioChem, 12(15), 2289–2293.

By: I. Koryakina n & G. Williams n 

co-author countries: United States of America 🇺🇸
author keywords: directed evolution; enzymes; polyketides; protein engineering; specificity
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Mutagenesis; Mutant Proteins / genetics; Mutant Proteins / metabolism; Polyketide Synthases / metabolism; Rhizobium / enzymology; Rhizobium / genetics; Substrate Specificity
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2011 journal article

Recombinant E. coli Prototype Strains for in Vivo Glycorandomization

ACS Chemical Biology, 6(1), 95–100.

By: G. Williams* , J. Yang *, C. Zhang * & J. Thorson *

co-author countries: United States of America 🇺🇸
MeSH headings : Biological Products / chemistry; Biological Products / metabolism; Carbohydrate Metabolism; Carbohydrates / chemistry; Catalysis; Escherichia coli / classification; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Glycosylation; Glycosyltransferases / chemistry; Glycosyltransferases / metabolism; Nucleotidyltransferases / chemistry; Nucleotidyltransferases / metabolism; Protein Engineering; Recombinant Proteins; Substrate Specificity
Sources: ORCID, Crossref
Added: December 15, 2020

2009 chapter

Natural Product Glycosyltransferases: Properties and Applications

In Advances in Enzymology - and Related Areas of Molecular Biology (pp. 55–119).

By: G. Williams*  & J. Thorson

MeSH headings : Aminocoumarins / metabolism; Aminoglycosides / metabolism; Anthracyclines / metabolism; Directed Molecular Evolution; Drug Design; Enediynes / metabolism; Enterobactin / metabolism; Erythromycin / metabolism; Glycosyltransferases / chemistry; Glycosyltransferases / metabolism; Ivermectin / analogs & derivatives; Ivermectin / metabolism; Lactams / metabolism; Macrolides / metabolism; Models, Molecular; Oleandomycin / metabolism; Protein Engineering; Teicoplanin / metabolism; Vancomycin / analogs & derivatives; Vancomycin / metabolism
Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution

Nature Protocols, 3(3), 357–362.

By: G. Williams*  & J. Thorson *

co-author countries: United States of America 🇺🇸
MeSH headings : Bacterial Proteins / genetics; Biomedical Engineering / methods; Directed Molecular Evolution / methods; Escherichia coli / genetics; Fluorescence; Gene Library; Glucosyltransferases / genetics; Glycosylation; Glycosyltransferases / analysis; Glycosyltransferases / metabolism; Mutagenesis / genetics; Mutation / genetics; Streptomyces antibioticus / enzymology
Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

Optimizing Glycosyltransferase Specificity via “Hot Spot” Saturation Mutagenesis Presents a Catalyst for Novobiocin Glycorandomization

Chemistry & Biology, 15(4), 393–401.

By: G. Williams* , R. Goff*, C. Zhang * & J. Thorson *

co-author countries: United States of America 🇺🇸
MeSH headings : Amino Acid Substitution; Amino Acids; Binding Sites; Catalysis; Directed Molecular Evolution; Fluorescence; Glycosylation; Glycosyltransferases / chemistry; Glycosyltransferases / genetics; Glycosyltransferases / metabolism; Kinetics; Mutagenesis, Site-Directed / methods; Novobiocin / metabolism; Oleandomycin / metabolism; Substrate Specificity
Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

Probing the Aglycon Promiscuity of an Engineered Glycosyltransferase

Angewandte Chemie International Edition, 47(46), 8889–8892.

By: R. Gantt*, R. Goff*, G. Williams*  & J. Thorson *

co-author countries: United States of America 🇺🇸
author keywords: carbohydrates; enzymes; glycosides; glycosylation; natural products
MeSH headings : Carbohydrate Metabolism; Carbohydrates / chemistry; Catalysis; Glycosyltransferases / metabolism; Molecular Structure; Protein Engineering; Streptomyces antibioticus / enzymology
Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

The impact of enzyme engineering upon natural product glycodiversification

Current Opinion in Chemical Biology, 12(5), 556–564.

By: G. Williams* , R. Gantt* & J. Thorson *

co-author countries: United States of America 🇺🇸
MeSH headings : Biological Products / chemical synthesis; Biological Products / chemistry; Biological Products / metabolism; Directed Molecular Evolution; Glycosylation; Glycosyltransferases / chemistry; Glycosyltransferases / metabolism; Humans; Protein Engineering / methods; Substrate Specificity
Sources: Crossref, ORCID
Added: December 22, 2020

2007 journal article

Stereochemically Complementary Biocatalysts Created by Directed Evolution

Synfacts, 2007(2), 0208–0208.

By: A. Nelson, G. Williams* , T. Woodhall, L. Farnsworth & A. Berry

Sources: Crossref, ORCID
Added: December 22, 2020

2006 journal article

Creation of a Pair of Stereochemically Complementary Biocatalysts

Journal of the American Chemical Society, 128(50), 16238–16247.

By: G. Williams* , T. Woodhall*, L. Farnsworth*, A. Nelson * & A. Berry *

co-author countries: United Kingdom of Great Britain and Northern Ireland 🇬🇧
MeSH headings : Aldehydes / chemistry; Biomimetic Materials / chemistry; Biomimetic Materials / metabolism; Catalysis; Evolution, Molecular; Genetic Variation / genetics; Kinetics; Lyases / chemistry; Lyases / genetics; Lyases / metabolism; Models, Molecular; Molecular Structure; Mutation / genetics; N-Acetylneuraminic Acid / chemistry; N-Acetylneuraminic Acid / metabolism; Stereoisomerism
Sources: Crossref, ORCID
Added: December 22, 2020

2005 journal article

Structure-guided saturation mutagenesis of N-acetylneuraminic acid lyase for the synthesis of sialic acid mimetics

Protein Engineering, Design and Selection, 18(5), 239–246.

By: G. Williams* , T. Woodhall, A. Nelson & A. Berry

author keywords: N-acetylneuraminic acid lyase; sialic acid mimetics; structure-guided saturation mutagenesis; synthesis
MeSH headings : Amides / metabolism; Amino Acid Substitution; Biomimetics; Escherichia coli / enzymology; Molecular Structure; Mutagenesis; N-Acetylneuraminic Acid / metabolism; Oxo-Acid-Lyases / chemistry; Oxo-Acid-Lyases / genetics; Protein Engineering; Structure-Activity Relationship; Substrate Specificity
Sources: Crossref, ORCID
Added: December 22, 2020

2003 journal article

Directed evolution: Creating new enzymes

The Biochemist, 25(4), 13–15.

By: G. Williams*  & A. Berry

Sources: NC State University Libraries, ORCID
Added: January 9, 2021

2003 journal article

Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution

Proceedings of the National Academy of Sciences, 100(6), 3143–3148.

By: G. Williams* , S. Domann*, A. Nelson * & A. Berry *

co-author countries: United Kingdom of Great Britain and Northern Ireland 🇬🇧
MeSH headings : Aldehyde-Lyases / chemistry; Aldehyde-Lyases / genetics; Aldehyde-Lyases / metabolism; Catalytic Domain; Directed Molecular Evolution; Escherichia coli / genetics; Fructosediphosphates / chemistry; Fructosediphosphates / metabolism; Hexosediphosphates / chemistry; Hexosediphosphates / metabolism; Kinetics; Models, Molecular; Mutation; Nuclear Magnetic Resonance, Biomolecular; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Stereoisomerism; Substrate Specificity
Sources: Crossref, ORCID
Added: December 22, 2020

Employment

Updated: January 16th, 2021 10:20

2020 - present

North Carolina State University Raleigh, North Carolina, US
Professor Chemistry

2015 - 2020

North Carolina State University Raleigh, North Carolina, US
Associate Professor Chemistry

2009 - 2015

North Carolina State University Raleigh, North Carolina, US
Assistant Professor Chemistry

Education

Updated: June 25th, 2014 11:36

University of Leeds Leeds, Leeds, GB
PhD Molecular Biology and Biochemistry

Funding History

Funding history based on the linked ORCID record. Updated: January 16th, 2021 10:24

grant March 17, 2020
Sery-CRISPR: A Novel Tool for Genome Editing
North Carolina Biotechnology Center
grant September 1, 2019 - August 31, 2022
MRI: Acquisition of a flow cytometer to promote microbe-focused research, training, and teaching at NC State University
Directorate for Biological Sciences
grant May 5, 2018 - February 28, 2022
Designer biosensors for directed evolution of macrolide biosynthetic enzymes
National Institute of General Medical Sciences
grant June 6, 2017
Massively Paralleled Engineering of Antibiotic-producing Microbes Via Made-to-measure Genetically-encoded Biosensors
North Carolina Biotechnology Center
grant June 15, 2013 - May 31, 2018
Synthetic Biology Approach to Regioselectively Modified Polyketides
National Institute of General Medical Sciences
grant April 1, 2012 - March 31, 2017
CAREER: Reprogramming Polyketide Biosynthesis
National Science Foundation