Gavin Williams

https://orcid.org/0000-0003-2172-9063

Synthetic biology, Natural products, Protein engineering, Metabolic engineering, Drug development

Works (48)

2020 journal article

An artificial pathway for polyketide biosynthesis

Nature Catalysis, 3(7), 536–538.

By: A. Gayen, L. Nichols & G. Williams

Sources: Web Of Science, ORCID, Crossref
Added: August 3, 2020

2020 journal article

Cheminformatics Analysis and Modeling with MacrolactoneDB

Scientific Reports, 10(1).

By: P. Zin, G. Williams & S. Ekins

Sources: Web Of Science, ORCID, Crossref
Added: September 7, 2020

2020 article

Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase

Kalkreuter, E., Bingham, K. S., Keeler, A. M., Lowell, A. N., Schmidt, J. J., Sherman, D. H., & Williams, G. J. (2020, April 25). (Vol. 4). Vol. 4.

By: E. Kalkreuter, K. Bingham, A. Keeler, A. Lowell, J. Schmidt, D. Sherman, G. Williams

Source: ORCID
Added: December 15, 2020

2020 journal article

SIME: synthetic insight-based macrolide enumerator to generate the V1B library of 1 billion macrolides

Journal of Cheminformatics, 12(1).

By: P. Zin, G. Williams & D. Fourches

Sources: Web Of Science, ORCID, Crossref
Added: April 27, 2020

2020 journal article

Synthetic biology enabling access to designer polyketides

Current Opinion in Chemical Biology, 58, 45–53.

By: A. Malico, L. Nichols & G. Williams

Sources: Crossref, ORCID, Web Of Science
Added: December 22, 2020

2020 article

Synthetic biology, combinatorial biosynthesis, and chemo-enzymatic synthesis of isoprenoids (September, 10.1007/s10295-020-02306-3, 2020)

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, Vol. 11.

By: A. Malico, M. Calzini, A. Gayen & G. Williams

Sources: Web Of Science, ORCID
Added: November 30, 2020

2020 journal article

Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids

Journal of Industrial Microbiology & Biotechnology, 47(9-10), 675–702.

By: A. Malico, M. Calzini, A. Gayen & G. Williams

Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: September 21, 2020

2019 journal article

An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism

ACS Synthetic Biology, 8(2), 232–238.

By: S. Lund, R. Hall & G. Williams

Sources: Web Of Science, ORCID, Crossref
Added: March 11, 2019

2019 journal article

Cover Feature: Probing the Substrate Promiscuity of Isopentenyl Phosphate Kinase as a Platform for Hemiterpene Analogue Production (ChemBioChem 17/2019)

ChemBioChem, 8.

By: S. Lund, T. Courtney & G. Williams

Source: ORCID
Added: December 15, 2020

2019 journal article

Development of a Genetically Encoded Biosensor for Detection of Polyketide Synthase Extender Units in Escherichia coli

ACS Synthetic Biology, 8(6), 1391–1400.

By: E. Kalkreuter, A. Keeler, A. Malico, K. Bingham, A. Gayen & G. Williams

Sources: Web Of Science, ORCID, Crossref
Added: July 15, 2019

2019 journal article

Engineering the Substrate Specificity of a Modular Polyketide Synthase for Installation of Consecutive Non-Natural Extender Units

Journal of the American Chemical Society, 141(5), 1961–1969.

By: E. Kalkreuter, J. CroweTipton, A. Lowell, D. Sherman & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: February 25, 2019

2019 journal article

Probing the Substrate Promiscuity of Isopentenyl Phosphate Kinase as a Platform for Hemiterpene Analogue Production

ChemBioChem, 20(17), 2217–2221.

By: S. Lund, T. Courtney & G. Williams

Sources: Web Of Science, ORCID, Crossref
Added: August 12, 2019

2018 journal article

Cheminformatics-based enumeration and analysis of large libraries of macrolide scaffolds

Journal of Cheminformatics, 10(1).

By: P. Zin, G. Williams & D. Fourches

Sources: NC State University Libraries, ORCID, Crossref
Added: December 3, 2018

2018 journal article

Development of Transcription Factor-Based Designer Macrolide Biosensors for Metabolic Engineering and Synthetic Biology

ACS Synthetic Biology, 7(1), 227–239.

By: C. Kasey, M. Zerrad, Y. Li, T. Cropp & G. Williams

Sources: Crossref, ORCID
Added: November 29, 2020

2018 journal article

Engineering enzymatic assembly lines for the production of new antimicrobials

Current Opinion in Microbiology, 45, 140–148.

By: E. Kalkreuter & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: January 21, 2019

2018 journal article

Extender Unit Promiscuity and Orthogonal Protein Interactions of an Aminomalonyl-ACP Utilizing Trans-Acyltransferase from Zwittermicin Biosynthesis

ACS Chemical Biology, 13(12), 3361–3373.

By: S. Carpenter & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: January 14, 2019

2017 journal article

Direct analysis of terpenes from biological buffer systems using SESI and IR-MALDESI

Analytical and Bioanalytical Chemistry, 410(3), 953–962.

By: M. Nazari, A. Malico, M. Ekelöf, S. Lund, G. Williams & D. Muddiman

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2017 journal article

Inversion of Extender Unit Selectivity in the Erythromycin Polyketide Synthase by Acyltransferase Domain Engineering

ACS Chemical Biology, 12(1), 114–123.

By: I. Koryakina, C. Kasey, J. McArthur, A. Lowell, J. Chemler, S. Li, D. Hansen, D. Sherman, G. Williams

Sources: ORCID, Crossref
Added: December 15, 2020

2017 journal article

Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

ACS Synthetic Biology, 6(3), 421–427.

By: E. Musiol-Kroll, F. Zubeil, T. Schafhauser, T. Härtner, A. Kulik, J. McArthur, I. Koryakina, W. Wohlleben ...

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Harnessing natural product assembly lines: structure, promiscuity, and engineering

Journal of Industrial Microbiology & Biotechnology, 43(2-3), 371–387.

By: C. Ladner & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Harnessing the promiscuity of natural product biosynthesis: A platform for engineering pathways with new specificities

Planta Medica, 81(11).

By: G. Williams

Sources: Crossref, ORCID
Added: December 22, 2020

2014 journal article

Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis

Rapid Communications in Mass Spectrometry, 28(23), 2511–2522.

By: S. Randall, I. Koryakina, G. Williams & D. Muddiman

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Intracellular Light-Activation of Riboswitch Activity

ChemBioChem, 15(9), 1346–1351.

By: S. Walsh, L. Gardner, A. Deiters & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Mapping a Ketosynthase:Acyl Carrier Protein Binding Interface via Unnatural Amino Acid-Mediated Photo-Cross-Linking

Biochemistry, 53(48), 7494–7502.

By: Z. Ye & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Reprogramming Acyl Carrier Protein Interactions of an Acyl-CoA Promiscuous trans-Acyltransferase

Chemistry & Biology, 21(5), 636–646.

By: Z. Ye, E. Musiol, T. Weber & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 conference paper

Synthetic Biology Approaches For Combinatorial Biosynthesis Of Polyketide Natural Products.

Abstracts of Papers for the American Chemical Society. Presented at the 247th National Spring Meeting of the American Chemical Society, Dallas, Texas, USA.

By: I. Koryakina & G. Williams

Event: 247th National Spring Meeting of the American Chemical Society at Dallas, Texas, USA on March 16, 2014

Source: NC State University Libraries
Added: December 30, 2020

2013 journal article

Engineering polyketide synthases and nonribosomal peptide synthetases

Current Opinion in Structural Biology, 23(4), 603–612.

By: G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2013 journal article

Promiscuity of a modular polyketide synthase towards natural and non-natural extender units

Organic & Biomolecular Chemistry, 11(27), 4449.

By: I. Koryakina, J. McArthur, M. Draelos & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2013 chapter

Reprogramming the Biosynthesis of Natural Products by Directed Evolution

In A. Kantardjieff, P. Asuri, J. L. Coffman, & K. Jayapal (Eds.), Developments in Biotechnology and Bioprocessing (pp. 147–163).

By: G. Williams, I. Koryakina, J. McArthur, M. Draelos, S. Randal & D. Muddimanl

Ed(s): A. Kantardjieff, P. Asuri, J. Coffman & K. Jayapal

Sources: Crossref, ORCID
Added: December 22, 2020

2012 chapter

Engineering Glycosyltransferases

In S. Lutz & U. T. Bornscheuer (Eds.), The Protein Engineering Handbook (Vol. 3, pp. 303–326). Weinheim: Wiley-HCH.

By: J. McArthur & G. Williams

Ed(s): S. Lutz & U. Bornscheuer

Source: NC State University Libraries
Added: December 30, 2020

2012 journal article

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

ACS Chemical Biology, 8(1), 200–208.

By: I. Koryakina, J. McArthur, S. Randall, M. Draelos, E. Musiol, D. Muddiman, T. Weber, G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2012 conference paper

Reprogramming the biosynthesis of natural products by directed evolution.

Abstracts of Papers of the American Chemical Society. Presented at the 243rd National Spring Meeting of the American Chemical Society, San Diego, CA, USA.

By: I. Koryakina, Z. Ye, J. McArthur & G. Williams

Event: 243rd National Spring Meeting of the American Chemical Society at San Diego, CA, USA on March 25, 2012

Source: NC State University Libraries
Added: December 30, 2020

2011 journal article

A High-Throughput Screen for Directed Evolution of the Natural Product Sulfotransferase LipB

Journal of Biomolecular Screening, 16(8), 845–851.

By: I. Koryakina, J. Neville, K. Nonaka, S. Van Lanen & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2011 journal article

A high-throughput screen for directed evolution of aminocoumarin amide synthetases

Analytical Biochemistry, 419(1), 61–66.

By: N. Parajuli & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2011 journal article

A photocrosslinking assay for reporting protein interactions in polyketide and fatty acid synthases

Molecular BioSystems, 7(11), 3152.

By: Z. Ye, M. Bair, H. Desai & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2011 journal article

Inside Cover: Mutant Malonyl-CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation (ChemBioChem 15/2011)

ChemBioChem, 12(15), 2230–2230.

By: I. Koryakina & G. Williams

Sources: ORCID, Crossref
Added: December 15, 2020

2011 journal article

Mutant Malonyl-CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation

ChemBioChem, 12(15), 2289–2293.

By: I. Koryakina & G. Williams

Sources: NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2011 journal article

Recombinant E. coli Prototype Strains for in Vivo Glycorandomization

ACS Chemical Biology, 6(1), 95–100.

By: G. Williams, J. Yang, C. Zhang & J. Thorson

Sources: ORCID, Crossref
Added: December 15, 2020

2009 chapter

Natural Product Glycosyltransferases: Properties and Applications

In Advances in Enzymology - and Related Areas of Molecular Biology (pp. 55–119).

By: G. Williams & J. Thorson

Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution

Nature Protocols, 3(3), 357–362.

By: G. Williams & J. Thorson

Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

Optimizing Glycosyltransferase Specificity via “Hot Spot” Saturation Mutagenesis Presents a Catalyst for Novobiocin Glycorandomization

Chemistry & Biology, 15(4), 393–401.

By: G. Williams, R. Goff, C. Zhang & J. Thorson

Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

Probing the Aglycon Promiscuity of an Engineered Glycosyltransferase

Angewandte Chemie International Edition, 47(46), 8889–8892.

By: R. Gantt, R. Goff, G. Williams & J. Thorson

Sources: Crossref, ORCID
Added: December 22, 2020

2008 journal article

The impact of enzyme engineering upon natural product glycodiversification

Current Opinion in Chemical Biology, 12(5), 556–564.

By: G. Williams, R. Gantt & J. Thorson

Sources: Crossref, ORCID
Added: December 22, 2020

2007 journal article

Stereochemically Complementary Biocatalysts Created by Directed Evolution

Synfacts, 2007(2), 0208–0208.

By: A. Nelson, G. Williams, T. Woodhall, L. Farnsworth & A. Berry

Sources: Crossref, ORCID
Added: December 22, 2020

2006 journal article

Creation of a Pair of Stereochemically Complementary Biocatalysts

Journal of the American Chemical Society, 128(50), 16238–16247.

By: G. Williams, T. Woodhall, L. Farnsworth, A. Nelson & A. Berry

Sources: Crossref, ORCID
Added: December 22, 2020

2005 journal article

Structure-guided saturation mutagenesis of N-acetylneuraminic acid lyase for the synthesis of sialic acid mimetics

Protein Engineering, Design and Selection, 18(5), 239–246.

By: G. Williams, T. Woodhall, A. Nelson & A. Berry

Sources: Crossref, ORCID
Added: December 22, 2020

2003 journal article

Directed evolution: Creating new enzymes

The Biochemist, 25(4), 13–15.

By: G. Williams & A. Berry

Sources: NC State University Libraries, ORCID
Added: January 9, 2021

2003 journal article

Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution

Proceedings of the National Academy of Sciences, 100(6), 3143–3148.

By: G. Williams, S. Domann, A. Nelson & A. Berry

Sources: Crossref, ORCID
Added: December 22, 2020

Employment

2020 - present

North Carolina State University Raleigh, North Carolina, US
Professor Chemistry

2015 - 2020

North Carolina State University Raleigh, North Carolina, US
Associate Professor Chemistry

2009 - 2015

North Carolina State University Raleigh, North Carolina, US
Assistant Professor Chemistry

Education

University of Leeds Leeds, Leeds, GB
PhD Molecular Biology and Biochemistry

Funding History

Funding history based on the linked ORCID record.

grant March 17, 2020
Sery-CRISPR: A Novel Tool for Genome Editing
North Carolina Biotechnology Center
grant September 1, 2019 - August 31, 2022
MRI: Acquisition of a flow cytometer to promote microbe-focused research, training, and teaching at NC State University
Directorate for Biological Sciences
grant May 5, 2018 - February 28, 2022
Designer biosensors for directed evolution of macrolide biosynthetic enzymes
National Institute of General Medical Sciences
grant June 6, 2017
Massively Paralleled Engineering of Antibiotic-producing Microbes Via Made-to-measure Genetically-encoded Biosensors
North Carolina Biotechnology Center
grant June 15, 2013 - May 31, 2018
Synthetic Biology Approach to Regioselectively Modified Polyketides
National Institute of General Medical Sciences
grant April 1, 2012 - March 31, 2017
CAREER: Reprogramming Polyketide Biosynthesis
National Science Foundation