Works (8)

Updated: August 29th, 2023 11:25

2011 journal article

Improving Proteome Coverage on a LTQ-Orbitrap Using Design of Experiments

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(4), 773–783.

By: G. Andrews n, R. Dean n, A. Hawkridge n & D. Muddiman n

author keywords: LTQ-Orbitrap XL MS/MS instrument parameters; Design of experiments; Fractional-factorial design; Saccharomyces cerevisiae; Proteomics
MeSH headings : Peptide Fragments; Peptide Mapping; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods; Temperature
TL;DR: The DOE approach represents a time- and cost-effective method for empirically optimizing MS-based proteomics workflows including sample preparation, LC conditions, and multiple instrument platforms. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Increasing proteome coverage with offline RP HPLC coupled to online RP nanoLC-MS

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 879(9-10), 610–614.

By: E. Gokce n, G. Andrews n, R. Dean n & D. Muddiman n

author keywords: RP HPLC; Fractionation methods; Proteomics; Mass spectrometry
MeSH headings : Chromatography, High Pressure Liquid / methods; Chromatography, Reverse-Phase / methods; Mass Spectrometry / methods; Nanotechnology; Peptide Fragments / analysis; Peptide Fragments / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / metabolism
TL;DR: The success of performing offline reversed phase high pressure liquid chromatography (HPLC) fractionation on a C18 2.0 mm×150 mm column at the peptide level with microliter per minute flow rates prior to online nano-flow reversed phase liquid Chromatography mass spectrometry (nanoLC-MS) using the well-studied fungus Saccharomyces cerevisiae is reported. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Performance Characteristics of a New Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer (TripleTOF 5600)

ANALYTICAL CHEMISTRY, 83(13), 5442–5446.

By: G. Andrews n, B. Simons, J. Young, A. Hawkridge n & D. Muddiman n

MeSH headings : Fungal Proteins / metabolism; Proteome; Saccharomyces cerevisiae / metabolism; Tandem Mass Spectrometry / instrumentation; Trypsin / metabolism
TL;DR: The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 377–389.

By: D. Muddiman n, G. Andrews n, D. Lewis n, J. Notey n & R. Kelly n

author keywords: Secretome; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Thermophile; Sample preparation
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Extracellular Space / metabolism; Gram-Positive Bacteria / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature
TL;DR: In an effort to define the extracellular proteome of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, several techniques were considered regarding sample processing to achieve the most in-depth analysis of secreted proteins. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 article

Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2 (vol 398, pg 377, 2010)

Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1837–1837.

By: G. Andrews n, D. Lewis n, J. Notey n, R. Kelly n & D. Muddiman*

Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(1), 391–404.

By: D. Muddiman n, G. Andrews n, D. Lewis n, J. Notey n & R. Kelly n

author keywords: Thermotoga maritima; Caldicellulosiruptor saccharolyticus; GeLC-MS2; Co-cultivation; Intracellular proteome; Spectral counting
MeSH headings : Bacterial Proteins / analysis; Bacterial Proteins / metabolism; Chemotaxis; Chromatography, Liquid; Coculture Techniques; Electrophoresis, Polyacrylamide Gel; Fourier Analysis; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Intracellular Space / metabolism; Proteome / analysis; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis / statistics & numerical data; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR: Altering in growth conditions of these bacterial thermophilic microorganisms offer a glimpse into the intricacy of microbial behavior and interaction as well as insight into microbial interaction and behavior. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 article

Part II: defining and quantifying individual and co-cultured intracellular proteomes of two thermophilic microorganisms by GeLC-MS2 and spectral counting (vol 398, pg 391, 2010)

Andrews, G., Lewis, D., Notey, J., Kelly, R., & Muddiman, D. (2010, October). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 398, pp. 1839–1839.

By: G. Andrews n, D. Lewis n, J. Notey n, R. Kelly n & D. Muddiman n

Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Coupling of a vented column with splitless nanoRPLC-ESI-MS for the improved separation and detection of brain natriuretic peptide-32 and its proteolytic peptides

Journal of Chromatography B, 877(10), 948–954.

By: G. Andrews n, C. Shuford n, J. Burnett*, A. Hawkridge n & D. Muddiman n

author keywords: Vented column; BNP-32; Splitless nanoLC; Tryptic digestion
MeSH headings : Amino Acid Sequence; Brain Chemistry; Chromatography, Liquid / methods; Molecular Sequence Data; Natriuretic Peptide, Brain / chemistry; Natriuretic Peptide, Brain / isolation & purification; Natriuretic Peptide, Brain / pharmacokinetics; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR: It is demonstrated that the use of free cysteine as an alkylation quenching agent and a secondary digestion within the digestion scheme can provide targeted tryptic peptides with increased abundances, which will serve to further augment the detection of BNP-32 by LC-MS. (via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

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