Guozhou Xu

Works (10)

Updated: April 5th, 2024 09:31

2023 journal article

The inhibitor of & kappa;B kinase & beta; (IKK & beta;) phosphorylates I & kappa;B & alpha; twice in a single binding event through a sequential mechanism

The inhibitor of & kappa;B kinase & beta; (IKK & beta;) phosphorylates I & kappa;B & alpha; twice in a single binding event through a sequential mechanism. JOURNAL OF BIOLOGICAL CHEMISTRY, 299(1).

By: A. Stephenson *, D. Taggart *, G. Xu*, J. Fowler*, H. Wu * & Z. Suo *

MeSH headings : Humans; I-kappa B Kinase / genetics; I-kappa B Kinase / metabolism; Kinetics; NF-kappa B / metabolism; NF-KappaB Inhibitor alpha / genetics; NF-KappaB Inhibitor alpha / metabolism; Phosphorylation; Protein Serine-Threonine Kinases / metabolism
TL;DR: The data suggest a model wherein, IKKβ phosphorylates IκBα at Ser32 followed by Ser36 within a single binding event, suggesting that phosphorylation of Ser32 increases the phosphorylated rate of Ser36. (via Semantic Scholar)
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Source: Web Of Science
Added: July 3, 2023

2021 journal article

Structure, dynamics, and regulation of TRF1-TIN2-mediated trans- and cis-interactions on telomeric DNA

JOURNAL OF BIOLOGICAL CHEMISTRY, 297(3).

By: H. Pan n, P. Kaur n, R. Barnes *, A. Detwiler *, S. Sanford *, M. Liu  n, P. Xu n, C. Mahn n ...

MeSH headings : Cell Adhesion Molecules / metabolism; Cell Adhesion Molecules / physiology; DNA / metabolism; DNA-Binding Proteins / metabolism; Humans; Microscopy, Atomic Force / methods; Models, Molecular; Multiprotein Complexes / metabolism; Protein Binding; Protein Isoforms / metabolism; Shelterin Complex / metabolism; Shelterin Complex / physiology; Telomere / metabolism; Telomere-Binding Proteins / metabolism; Telomere-Binding Proteins / physiology; Telomeric Repeat Binding Protein 1 / metabolism; Telomeric Repeat Binding Protein 1 / physiology; Telomeric Repeat Binding Protein 2 / metabolism; Telomeric Repeat Binding Protein 2 / physiology
TL;DR: Analysis of DNA molecular structures promoted by TRF1-TIN2 interaction supports a molecular model in which protein assemblies at telomeres are heterogeneous with distinct subcomplexes and full shelterin complexes playing distinct roles in telomere protection and elongation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 26, 2021

2020 journal article

Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)

PROTEIN JOURNAL, 39(5), 461–471.

By: K. Trihemasava  n, S. Chakraborty n, K. Blackburn  n & G. Xu n

author keywords: LRR-RLK; Post-translational modifications; N-glycosylation; LC– MS; Phylogenetic analysis
MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / biosynthesis; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Gene Expression; Phylogeny; Protein Kinases / biosynthesis; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / isolation & purification; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification
TL;DR: This work describes expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC–MS/MS and computational sequence-based analyses. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: November 16, 2020

2019 review

Plant Leucine-Rich Repeat Receptor Kinase (LRR-RK): Structure, Ligand Perception, and Activation Mechanism

[Review of ]. MOLECULES, 24(17).

By: S. Chakraborty n, B. Nguyen  n, S. Wasti n & G. Xu n

author keywords: receptor kinase; LRR-RK; signaling; X-ray crystallography
MeSH headings : Leucine / chemistry; Ligands; Models, Molecular; Peptides / chemistry; Peptides / genetics; Peptides / metabolism; Plant Growth Regulators; Plant Physiological Phenomena; Plant Proteins / chemistry; Plant Proteins / physiology; Plants / genetics; Plants / metabolism; Protein Binding; Protein Conformation; Protein Interaction Domains and Motifs; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / physiology; Signal Transduction; Structure-Activity Relationship
TL;DR: The structures of many plant LRR-RK receptors in complex with corresponding ligands have been solved by X-ray crystallography, revealing new mechanisms of ligand-induced receptor kinase activation. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: October 21, 2019

2018 journal article

Mechanism of vaccinia viral protein B14–mediated inhibition of IκB kinase β activation

Journal of Biological Chemistry, 293(26), 10344–10352.

By: Q. Tang  n, S. Chakraborty  n & G. Xu n

author keywords: molecular docking; autophosphorylation; protein kinase; virus; inhibition mechanism; B14; IKK; protein kinase; trans autophosphorylation; vaccinia virus
MeSH headings : Animals; Enzyme Activation; Humans; I-kappa B Proteins / chemistry; I-kappa B Proteins / metabolism; Models, Molecular; Protein Binding; Protein Domains; Protein Structure, Quaternary; Vaccinia virus; Viral Proteins / metabolism; Xenopus
TL;DR: The B14–IKKβ interaction sterically hinders direct contact between the kinase domains of IKK β in the IKK complex, containing IKKβ, IKKα, and NEMO in human cells, thereby inhibiting NF-κB signaling. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Crossref
Added: November 30, 2020

2018 journal article

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (138).

By: S. Chakraborty n, K. Trihemasava  n & G. Xu n

author keywords: Biochemistry; Issue 138; Insect cell; baculovirus; expression vector; secretory protein; signal peptide; GP67; hemolin; bacmid; transfection; cell culture; glycosylation; protein expression; protein crystallization
MeSH headings : Animals; Baculoviridae / genetics; Baculoviridae / metabolism; Genetic Vectors; Insecta / genetics; Plant Proteins / metabolism
TL;DR: This newly designed modified vector system successfully produced a high yield of soluble recombinant secreted plant receptor proteins of Arabidopsis thaliana, and is an improved tool that can potentially be used for the expression of recombinantsecretory proteins in the animal kingdom. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: October 16, 2018

2018 journal article

The Extracellular Domain of Pollen Receptor Kinase 3 is structurally similar to the SERK family of co-receptors

SCIENTIFIC REPORTS, 8.

By: S. Chakraborty n, H. Pan n, Q. Tang n, C. Woolard n & G. Xu n

MeSH headings : Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Flowers / growth & development; Ovule / metabolism; Plant Proteins / metabolism; Pollen / metabolism; Pollen Tube / growth & development; Pollination; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / metabolism; Reproduction / physiology; Signal Transduction / physiology
TL;DR: The authors' structural analyses of PRK3 have provided a template for future functional studies of the PRK family of LRR-RLK receptors in the regulation of pollen tube development, and determined the crystal structure of the extracellular domain (ecd) ofPRK3 at 2.5 Å, which resembles the SERKfamily of plant co-receptors. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Differential CLE peptide perception by plant receptors implicated from structural and functional analyses of TDIF-TDR interactions

PLOS ONE, 12(4).

By: Z. Li n, S. Chakraborty n & G. Xu n

MeSH headings : Amino Acid Sequence; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Binding Sites; Molecular Structure; Protein Binding; Protein Kinases / metabolism; Sequence Homology, Amino Acid
TL;DR: These studies will open different unknown avenues of TDR-TDIF signaling pathways that will enhance knowledge in this field highlighting the receptor ligand interaction, receptor activation, signaling network, modes of action and will serve as a structure function relationship model between the ligand and the receptor for various similar leucine-rich repeat receptor-like kinases (LRR-RLKs). (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Evidence for M1-Linked Polyubiquitin-Mediated Conformational Change in NEMO

JOURNAL OF MOLECULAR BIOLOGY, 429(24), 3793–3800.

By: A. Hauenstein *, G. Xu*, V. Kabaleeswaran* & H. Wu*

author keywords: Innate immune signaling; Small-angle X-ray scattering; Protein biochemistry; Size exclusion chromatography
MeSH headings : Binding Sites; Humans; I-kappa B Kinase / chemistry; I-kappa B Kinase / metabolism; NF-kappa B / metabolism; Polyubiquitin / metabolism; Protein Binding; Protein Conformation; Signal Transduction; Ubiquitination
TL;DR: Observations suggest that NEMO may be auto-inhibited in the resting state by intramolecular interactions and that during signaling, NEMo may be allosterically activated by binding to long M1-linked polyubiquitin chains. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2016 journal article

X-ray crystallographic studies of the extracellular domain of the first plant ATP receptor, DORN1, and the orthologous protein from Camelina sativa

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 72, 782–787.

By: Z. Li n, S. Chakraborty n & G. Xu n

author keywords: plant ATP receptor; DORN1; lectin receptor kinase I.9; glycosylation; Arabidopsis thaliana; Camelina sativa
MeSH headings : Adenosine Triphosphate / chemistry; Adenosine Triphosphate / metabolism; Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis / metabolism; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Baculoviridae / genetics; Baculoviridae / metabolism; Brassicaceae / chemistry; Brassicaceae / metabolism; Cloning, Molecular; Crystallization; Crystallography, X-Ray; Gene Expression; Glycosylation; Plasmids / chemistry; Plasmids / metabolism; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; X-Ray Diffraction
TL;DR: The extracellular domain of the A. thaliana ATP receptor DORN1 has been expressed and purified; a protein orthologous to Dorn1 from C. sativa has been crystallized and its X-ray diffraction data have been collected. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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