Works (11)

Updated: July 5th, 2023 15:53

2023 article

UGT76F1 glycosylates an isomer of the C7-necic acid component of pyrrolizidine alkaloids in Arabidopsis thaliana

Chen, H.-Y., Li, X., Chapple, C., Dilkes, B., & Li, X. (2023, April 12). PLANT JOURNAL, Vol. 4.

By: H. Chen n, X. Li n, C. Chapple*, B. Dilkes* & X. Li n

author keywords: Arabidopsis thaliana; metabolic GWA; plant specialized metabolism; UDP-glycosyltransferase
TL;DR: Feeding norvaline greatly enhances the accumulation of 2-hydroxy-2-(1-Hydroxyethyl)pentanoic acid glucoside in wild type but not the UGT76F1 knockout mutant plants, providing evidence for an orthologous C7-necic acid biosynthetic pathway in Arabidopsis despite the apparent lack of pyrrolizidine alkaloids. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 31, 2023

2020 journal article

An Efficient Stevia rebaudiana Transformation System and In vitro Enzyme Assays Reveal Novel Insights into UGT76G1 Function

SCIENTIFIC REPORTS, 10(1).

By: Q. Wu n, C. La Hovary n, H. Chen n, X. Li n, H. Eng n, V. Vallejo*, R. Qu n, R. Dewey n

MeSH headings : Alternative Splicing; Glycosyltransferases / biosynthesis; Glycosyltransferases / genetics; Isoenzymes / biosynthesis; Isoenzymes / genetics; Plant Proteins / biosynthesis; Plant Proteins / genetics; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / genetics; Stevia / enzymology; Stevia / genetics; Transformation, Genetic
TL;DR: A highly efficient Agrobacterium-mediated stable transformation system using axillary shoots as the initial explant is reported and evidence suggesting that alternative and/or aberrant splicing may serve to influence the ability of the plant to produce functional UGT76G1 transcripts, and possibly produce enzyme variants within the plant is found. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2020 journal article

An importin-beta-like protein mediates lignin-modification-induced dwarfism in Arabidopsis

PLANT JOURNAL, 102(6), 1281–1293.

By: C. Panda n, X. Li n, A. Wager n, H. Chen n & X. Li n

author keywords: Arabidopsis thaliana; importin; lignin; MYB4; plant growth; ref8; suppressor mutant
MeSH headings : Adaptor Proteins, Signal Transducing / metabolism; Adaptor Proteins, Signal Transducing / physiology; Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Cell Division; Gene Expression Regulation, Plant; Lignin / metabolism; Repressor Proteins / metabolism; Repressor Proteins / physiology; Transcription Factors / metabolism; Transcription Factors / physiology; beta Karyopherins / metabolism
TL;DR: It is shown that defects in both cell division and cell expansion underlie the dwarfism of an Arabidopsis lignin mutant ref8, and the identification of a GIR1 gene from a suppressor screen is reported, suggesting that the growth rescue effect of gir1 is likely due to compromised MYB4 transport and function. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 10, 2020

2018 journal article

Comprehensive transcriptome analyses correlated with untargeted metabolome reveal differentially expressed pathways in response to cell wall alterations

PLANT MOLECULAR BIOLOGY, 96(4-5), 509–529.

By: N. Reem*, H. Chen n, M. Hur*, X. Zhao*, E. Wurtele*, X. Li n, L. Li*, O. Zabotina*

author keywords: Arabidopsis thaliana; Cell wall signaling; Metabolomics; Systems biology; Transcriptomics
MeSH headings : Arabidopsis / genetics; Cell Wall / metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant; Hydrolases / metabolism; Metabolome / genetics; Plants, Genetically Modified; Reproducibility of Results; Stress, Physiological / genetics; Transcription Factors / metabolism; Transcriptome / genetics
TL;DR: Combining the transcriptomics and metabolomics datasets increased the power of pathway prediction, and demonstrated the complexity of pathways involved in cell wall-mediated signaling, elucidate how primary metabolism responds to extracellular stimuli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Host-plant induced changes in microbial community structure and midgut gene expression in an invasive polyphage (Anoplophora glabripennis)

SCIENTIFIC REPORTS, 8.

By: E. Scully*, S. Geib*, C. Mason*, J. Carlson*, M. Tien*, H. Chen*, S. Harding, C. Tsai*, K. Hoover*

MeSH headings : Acer; Animals; Biodiversity; Coleoptera / genetics; Coleoptera / metabolism; Coleoptera / microbiology; Coleoptera / physiology; Digestion; Fatty Acids / metabolism; Feeding Behavior; Gastrointestinal Microbiome; Gene Expression Regulation; Genes, Insect / genetics; Introduced Species; Populus; Sequence Analysis, RNA
TL;DR: It is shown that feeding in two suitable hosts altered the expression levels of multicopy genes linked to digestion and detoxification, and feeding in a host with documented resistance induced changes in the transcriptome and community beyond what was observed in insects reared in P. nigra. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Identification of a residue responsible for UDP-sugar donor selectivity of a dihydroxybenzoic acid glycosyltransferase from Arabidopsis natural accessions

PLANT JOURNAL, 89(2), 195–203.

By: H. Chen n & X. Li n

author keywords: plant secondary metabolism; UGT; substrate specificity; natural variation; Arabidopsis thaliana; dihydroxybenzoic acid
MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Gentisates / metabolism; Glucuronosyltransferase / chemistry; Glucuronosyltransferase / genetics; Glucuronosyltransferase / metabolism; Hydroxybenzoates / metabolism; Mutagenesis, Site-Directed; Polymorphism, Genetic; Structural Homology, Protein; Structure-Activity Relationship; Substrate Specificity
TL;DR: Natural variation in Arabidopsis UGT89A2 guided the discovery that a single amino acid residue change determines UDP‐sugar donor selectivity of the dihydroxybenzoic acid glycosyltransferase, providing new insights into UDP‐xylose specificity of a class of enzymes important to specialized metabolism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Importance of Cellulosic Fines Relative to the Dewatering Rates of Fiber Suspensions

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 48(20), 9106–9112.

Source: Web Of Science
Added: August 6, 2018

2001 journal article

Human milk antibacterial factors - The effect of temperature on defense systems

Bioactive Components of Human Milk (Advances in Experimental Medicine and Biology; v. 501), 341–348.

By: H. Chen n & J. Allen n

TL;DR: Reactive IgA antibodies againstListeriaantigens were demonstrated by enzyme immunoassay in some human milk samples and sIgA activity againstEscherichia coliO antigens was significantly decreased by heat treatments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Developing a dry-cured ham nutritional database

Journal of Food Composition and Analysis, 10(3), 190–204.

By: H. Chen n, D. Pilkington n, J. Tharrington n & J. Allen n

Source: NC State University Libraries
Added: August 6, 2018

1992 journal article

FRACTIONATION OF BUTTER OIL BY SUPERCRITICAL CARBON-DIOXIDE

JOURNAL OF DAIRY SCIENCE, 75(10), 2659–2669.

By: H. Chen n, S. Schwartz n & G. Spanos

author keywords: BUTTER OIL; SUPERCRITICAL CARBON DIOXIDE; FRACTIONATION
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1991 journal article

Fractionation of butter oil with supercritical carbon dioxide

Journal of Dairy Science, 74, 130.

By: H. Chen & S. Schwartz

Source: NC State University Libraries
Added: August 6, 2018

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