@article{chu_sripada_reese_bhandari_adams_sly_crapanzano_menegatti_2021, title={Purification of polyclonal immunoglobulin G from human serum using peptide-based adsorbents}, volume={10}, ISSN={["1547-5905"]}, DOI={10.1002/aic.17482}, abstractNote={Abstract}, journal={AICHE JOURNAL}, author={Chu, Wenning and Sripada, Sobhana A. and Reese, Hannah R. and Bhandari, Dipendra and Adams, Augustus and Sly, Jae and Crapanzano, Michael and Menegatti, Stefano}, year={2021}, month={Oct} }
 @article{reese_bordelon_odeh_broussard_kormos_murphy_shanahan_menegatti_2020, title={Purification of animal immunoglobulin G (IgG) using peptoid affinity ligands}, ISBN={1520-6033}, DOI={10.1002/btpr.2994}, abstractNote={Abstract}, journal={BIOTECHNOLOGY PROGRESS}, author={Reese, Hannah and Bordelon, Tee and Odeh, Fuad and Broussard, Amanda and Kormos, Chad and Murphy, Andrew and Shanahan, Calvin and Menegatti, Stefano}, year={2020} }
 @article{bacon_bowen_reese_rao_menegatti_2020, title={Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands}, volume={22}, ISSN={["2156-8944"]}, DOI={10.1021/acscombsci.0c00171}, abstractNote={This work presents the first use of yeast-displayed protein targets for screening mRNA-display libraries of cyclic and linear peptides. The WW domains of Yes-Associated Protein 1 (WW-YAP) and mitochondrial import receptor subunit TOM22 were adopted as protein targets. Yeast cells displaying WW-YAP or TOM22 were magnetized with iron oxide nanoparticles to enable the isolation of target-binding mRNA-peptide fusions. Equilibrium adsorption studies were conducted to estimate the binding affinity (KD) of select WW-YAP-binding peptides: KD values of 37 and 4 μM were obtained for cyclo[M-AFRLC-K] and its linear cognate, and 40 and 3 μM for cyclo[M-LDFVNHRSRG-K] and its linear cognate, respectively. TOM22-binding peptide cyclo[M-PELNRAI-K] was conjugated to magnetic beads and incubated with yeast cells expressing TOM22 and luciferase. A luciferase-based assay showed a 4.5-fold higher binding of TOM22+ yeast compared to control cells. This work demonstrates that integrating mRNA- and yeast-display accelerates the discovery of peptide ligands.}, number={12}, journal={ACS COMBINATORIAL SCIENCE}, author={Bacon, Kaitlyn and Bowen, John and Reese, Hannah and Rao, Balaji M. and Menegatti, Stefano}, year={2020}, month={Dec}, pages={738–744} }