Works (165)

Updated: April 22nd, 2024 05:27

2024 article

Complete genome sequences of <i>Lactobacillus acidophilus</i> strain P42 and <i>Limosilactobacillus reuteri</i> strain P43 isolated from chicken cecum

Hassan, H. M., Mendoza, M., & Dickey, A. N. (2024, March 19). (J. C. D. Hotopp, Ed.). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 3.

By: H. Hassan n, M. Mendoza n & A. Dickey n

Ed(s): J. Hotopp

author keywords: complete genome; PacBio HiFi reads; L. acidophilus P42; L. reuteriP43; poultry
TL;DR: The complete circular genome sequences of Lactobacillus acidophilus strain P42 and Limosilactobacillus reuteri strain P43 isolated from chicken cecal samples are reported and could potentially serve as poultry probiotic strains. (via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 22, 2024

2024 journal article

In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks

POULTRY SCIENCE, 103(1).

By: S. Sharma*, R. Kulkarni n, S. Sharif*, H. Hassan n, M. Alizadeh*, S. Pratt*, K. Abdelaziz*

author keywords: chicken; probiotics; Lactobacillus; cytokine; bursa of Fabricius
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 2, 2024

2024 article

Modulation of functional activity of heat-stressed chicken macrophages by poultry-derived probiotic lactobacilli

Abdelaziz, K., Nixon, T., Joye, A., Hassan, H., Alizadeh, M., Sharif, S., & Kulkarni, R. R. (2024, April 9). CANADIAN JOURNAL OF ANIMAL SCIENCE, Vol. 4.

By: K. Abdelaziz*, T. Nixon*, A. Joye*, H. Hassan n, M. Alizadeh*, S. Sharif*, R. Kulkarni n

author keywords: chicken; Lactobacillus; macrophage; cytokine; TLR; heat stress
Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024

2022 journal article

On 'Intracellular production of superoxide radical and of hydrogen peroxide by redox active compounds' by H. Moustafa Hassan, Irwin Fridovich

Archives of Biochemistry and Biophysics.

By: H. Hassan n

MeSH headings : Catalase / metabolism; Escherichia coli / metabolism; Free Radicals; Hydrogen Peroxide / pharmacology; Oxidation-Reduction; Oxygen; Superoxide Dismutase / metabolism; Superoxides
TL;DR: This commentary discusses how the idea of employing redox cycling compounds to generate partially reduced oxygen species to cause oxidative stress in the model organism, Escherichia coli, was born. (via Semantic Scholar)
Source: ORCID
Added: April 27, 2022

2021 journal article

Role of the Mn-Catalase in Aerobic Growth of Lactobacillus plantarum ATCC 14431

Applied Microbiology.

By: T. Peacock n & H. Hassan n

TL;DR: The physiological role of Mn-catalase (MnKat) in Lactobacillus plantarum ATCC 14431 is investigated and showed that the MnKat is critical for aerobic growth, protection against H2O2, and maintenance of the rod-shaped cell morphology under aerobic conditions. (via Semantic Scholar)
Source: ORCID
Added: December 21, 2021

2020 journal article

Attenuated Salmonella enterica Serovar Typhimurium, Strain NC983, Is Immunogenic, and Protective against Virulent Typhimurium Challenges in Mice

VACCINES, 8(4).

By: B. Troxell n, M. Mendoza n, R. Ali n, M. Koci n & H. Hassan n

author keywords: Salmonella typhimurium; NC983; vaccine; in vivo; infection; C57BL/6 mice; BALB/c mice; IgG
TL;DR: Oral delivery of strain NC983 was capable of fully protecting C57BL/6 and BALB/c mice against challenge with virulent Typhimurium, and results indicate that strainNC983 is a potential live attenuated vaccine strain that warrants further development. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 19, 2021

2020 journal article

Complete Genome Sequences of Lactobacillus Strains C25 and P38, Isolated from Chicken Cecum

Microbiology Resource Announcements, 9(39).

By: H. Hassan n, M. Mendoza n, M. Rezvani n, M. Koci n, A. Dickey n & E. Scholl n

Ed(s): D. Rasko

TL;DR: The complete circular genome sequences of Lactobacillus crispatus strain C25, its plasmid, and LactOBacillus animalis strain P38; both strains were isolated from the cecum of 4-week-old chickens represent potential probiotic strains for poultry. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
15. Life on Land (Web of Science)
Source: ORCID
Added: September 24, 2020

2020 journal article

Complete Genome Sequences of Six Lactobacilli Isolated from American Quarter Horses

MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(47).

By: R. Meinders n, M. Mendoza n, A. Dickey n, E. Scholl n & H. Hassan n

Ed(s): D. Rasko

TL;DR: The complete circular genome sequences of six Lactobacillus strains and their plasmids, if any, from the fecal material of quarter horses at different ages are reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 4, 2021

2017 journal article

A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome

BMC MICROBIOLOGY, 17.

By: I. Allali*, J. Arnold*, J. Roach*, M. Cadenas*, N. Butz*, H. Hassan n, M. Koci n, A. Ballou n ...

author keywords: 16S rRNA amplicon sequencing; microbiome analysis; microbiome; microbiome composition; next generation sequencing platforms; Bioinformatics pipeline; NGS bias
MeSH headings : Analysis of Variance; Animals; Bacteria / classification; Bacteria / genetics; Base Sequence; Biodiversity; Cecum / microbiology; Chickens / microbiology; Computational Biology / instrumentation; Computational Biology / methods; DNA, Bacterial / analysis; DNA, Bacterial / genetics; DNA, Bacterial / isolation & purification; Gastrointestinal Microbiome / genetics; Gene Library; High-Throughput Nucleotide Sequencing / instrumentation; High-Throughput Nucleotide Sequencing / methods; Microbial Consortia / genetics; Multivariate Analysis; Phylogeny; RNA, Ribosomal, 16S / genetics; Reproducibility of Results; Statistics as Topic
TL;DR: Overall microbiome compositional profiles were comparable between platforms; however, average relative abundance of specific taxa varied depending on sequencing platform, library preparation method, and bioinformatics analysis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

An Attenuated Salmonella enterica Serovar Typhimurium Strain and Galacto-Oligosaccharides Accelerate Clearance of Salmonella Infections in Poultry through Modifications to the Gut Microbiome

Applied and Environmental Microbiology, 84(5).

By: M. Azcarate-Peril*, N. Butz*, M. Cadenas*, M. Koci n, A. Ballou n, M. Mendoza n, R. Ali n, H. Hassan n

Ed(s): J. Schottel

author keywords: chicken gut microbiome; gut microbiome modulation; prebiotics
MeSH headings : Animals; Bacterial Vaccines / pharmacology; Chickens; Female; Gastrointestinal Microbiome / drug effects; Oligosaccharides / administration & dosage; Poultry Diseases / microbiology; Poultry Diseases / prevention & control; Prebiotics / administration & dosage; Random Allocation; Salmonella Infections, Animal / microbiology; Salmonella Infections, Animal / prevention & control; Salmonella typhimurium / immunology; Vaccines, Attenuated / pharmacology
TL;DR: It is demonstrated that a manipulation of the chicken gut microbiota by the administration of an attenuated Salmonella strain or prebiotic galacto-oligosaccharides (GOS) can promote resistance to Salmonellosis via increases of beneficial microorganisms that translate into a less hospitable gut microenvironment. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2017 journal article

Impact of Dietary Galacto-Oligosaccharide (GOS) on Chicken’s Gut Microbiota, Mucosal Gene Expression, and Salmonella Colonization

Frontiers in Veterinary Science, 4.

By: R. Hughes n, R. Ali n, M. Mendoza n, H. Hassan n & M. Koci n

author keywords: chickens; immune; Salmonella; prebiotic; galacto-oligosaccharides
TL;DR: Investigation of the effect of the prebiotic galacto-oligosaccharide (GOS) on the cecal microbiome and ultimately the carriage of Salmonella demonstrated that treatment with thePrebiotic GOS can modify both c Cecal tonsil gene expression and the cECal microbiome, suggesting that this type of treatment may be useful as a tool for altering the carriage in poultry. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 19, 2018

2016 journal article

Complete Genome Sequence of NC983, a Live Attenuated Strain of Salmonella enterica Serovar Typhimurium

Genome Announcements, 4(5).

By: B. Troxell n, R. Fink*, A. Dickey n, E. Scholl n & H. Hassan n

TL;DR: The genome sequence of the nontyphoidal S. enterica serovar Typhimurium mutant strain NC983 is presented, a potential vaccine candidate, and a potential source of infection for Salmonella serovars. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: June 22, 2021

2016 report

Designer Probiotic Lactobacilli for use in Farm Animals and Methods for Enrichment and Isolation from any Animal Species or Human

(Invention Disclosure No. 17-063).

By: H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

2016 journal article

Development of the Chick Microbiome: How Early Exposure Influences Future Microbial Diversity

Frontiers in Veterinary Science, 3.

By: A. Ballou n, R. Ali n, M. Mendoza n, J. Ellis*, H. Hassan n, W. Croom n, M. Koci n

author keywords: chicken; microbiome development; Salmonella; probiotic; gut development
TL;DR: Assessment of the development of the cecal microbiome in chicks from hatch to 28 days of age with and without a live Salmonella vaccine and/or probiotic supplement demonstrates that these live microbial treatments do impact theDevelopment of the bacterial taxa found in the growing chicks. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 19, 2018

2016 journal article

Draft Genome Sequence of Lactobacillus crispatus C25 Isolated from Chicken Cecum

Genome Announcements, 4(6).

By: M. Rezvani n, M. Mendoza n, M. Koci n, C. Daron n, J. Levy* & H. Hassan n

TL;DR: The genome sequence of Lactobacillus crispatus strain C25, originally isolated from the cecum of 4-week-old chicken fed a standard diet, is presented, representing a potential probiotic strain for poultry. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 19, 2018

2016 journal article

Draft Genome Sequences of Lactobacillus animalis Strain P38 and Lactobacillus reuteri Strain P43 Isolated from Chicken Cecum

Genome Announcements, 4(6).

By: M. Rezvani n, M. Mendoza n, M. Koci n, C. Daron n, J. Levy* & H. Hassan n

TL;DR: These indigenous Lactobacillus isolates are potential probiotic organisms for poultry after isolated from the cecum content of a 4-week old chicken fed a diet supplemented with the prebiotic β(1-4)galacto-oligosaccharide (GOS). (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: December 19, 2018

2016 report

Engineered Salmonella Serovar Typhimurium Strains, Compositions Thereof, and Methods of Use

(US Provisional Patent No. 62/368,507).

By: H. Hassan & S. Troxell

Source: NC State University Libraries
Added: August 28, 2021

2016 chapter

Interplay Between O2 and Iron in Gene Expression: Environmental Sensing by FNR, ArcA, and Fur in Bacteria

In F. J. de Bruijn (Ed.), Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria, I&II (pp. 1079–1089).

By: B. Troxell n & H. Hassan n

Ed(s): F. de Bruijn

Sources: Crossref, NC State University Libraries
Added: August 13, 2021

2016 conference paper

The Chicken Gut Microbiome and Salmonella

Presented at the USDA-NIFA –Annual Program Directors Meeting, St. Louis, MO.

By: H. Hassan

Event: USDA-NIFA –Annual Program Directors Meeting at St. Louis, MO on July 30, 2016

Source: NC State University Libraries
Added: August 13, 2021

2016 speech

Use of Probiotics and prebiotics in Poultry

Hassan, H. (2016, October). Presented at the Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

By: H. Hassan

Event: at Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

Source: NC State University Libraries
Added: March 12, 2022

2016 report

Utilization of Hydroxyl Radicalfor Accelerated Shelf-life Testing

(Invention Disclosure No. 16-275).

By: H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

2015 journal article

Biology of reactive oxygen species, oxidative stress, and antioxidants in lactic acid bacteria

Probiotics and Prebiotics: Current Research and Future Trends, 205–218.

By: M. Leite, B. Troxell, J. Bruno-Bárcena & H. Hassan*

Contributors: M. Leite, B. Troxell, J. Bruno-Barcena* & H. Hassan*

Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Mitochondrial DNA Fragmentation as a Molecular Tool to Monitor Thermal Processing of Plant-Derived, Low-Acid Foods, and Biomaterials

Journal of Food Science, 80(8), M1804–M1814.

author keywords: mitochondrial DNA; quantitative PCR; thermal processing
MeSH headings : Clostridium botulinum / isolation & purification; DNA Damage; DNA Fragmentation; DNA Primers; DNA, Mitochondrial / genetics; DNA, Mitochondrial / isolation & purification; DNA, Plant / genetics; DNA, Plant / isolation & purification; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Fruit; Genes, Plant; Geobacillus stearothermophilus / isolation & purification; Hot Temperature; Ipomoea batatas / microbiology; Linear Models; Plant Proteins / genetics; Reproducibility of Results; Spores, Bacterial / isolation & purification; Vegetables
TL;DR: Data is presented as proof-of-concept for a molecular tool that can be used as a rapid, presumptive method for monitoring thermal processing in low-acid plant products. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

Mitochondrial DNA Fragmentation to Monitor Processing Parameters in High Acid, Plant-Derived Foods

Journal of Food Science, 80(12), M2892–M2898.

author keywords: high-acid foods; mitochondrial DNA; quantitative PCR; thermal processing
MeSH headings : Acids; Bacteria / growth & development; Cucumis sativus / genetics; DNA Fragmentation; DNA, Mitochondrial; DNA, Plant / analysis; Fermentation; Food Analysis; Food Handling / methods; Food Microbiology; Food Preservation / methods; Fruit; Genes, Mitochondrial; Genes, Plant; Humans; Hydrogen-Ion Concentration; Solanum lycopersicum / genetics; Pasteurization; Plants, Edible / genetics; Temperature; Vegetables
TL;DR: MtDNA fragmentation was shown to be a potential new tool to characterize low temperature (<100 °C) high acid processes (pH < 4.6), nonthermal processes such as vegetable fermentation and holding times of acidified, plant-derived products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2015 journal article

Poultry Body Temperature Contributes to Invasion Control through Reduced Expression of Salmonella Pathogenicity Island 1 Genes in Salmonella enterica Serovars Typhimurium and Enteritidis

Applied and Environmental Microbiology, 81(23), 8192–8201.

By: B. Troxell n, N. Petri n, C. Daron n, R. Pereira*, M. Mendoza n, H. Hassan n, M. Koci n

Contributors: B. Troxell n, N. Petri n, C. Daron n, R. Pereira*, M. Mendoza n, H. Hassan n, M. Koci n

Ed(s): C. Elkins

MeSH headings : Animals; Cecum / microbiology; Cecum / physiology; Chickens; Genomic Islands; Liver / microbiology; Liver / physiology; Poultry Diseases / genetics; Poultry Diseases / metabolism; Poultry Diseases / microbiology; Salmonella Infections, Animal / genetics; Salmonella Infections, Animal / metabolism; Salmonella Infections, Animal / microbiology; Salmonella enteritidis / genetics; Salmonella enteritidis / physiology; Salmonella typhimurium / genetics; Salmonella typhimurium / physiology; Spleen / microbiology; Spleen / physiology; Temperature
TL;DR: In vivo experiments using chicks demonstrated that numbers of viable S. Typhimurium or S. Enteritidis bacteria within the liver and spleen organ sites were ≥4 orders of magnitude lower than those within the ceca, indicating poor survival within these tissues or a reduced capacity to traverse the host epithelial layer and reach liver/spleen sites or both. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 report

A vector for vaccination against Lyme Disease and other insect transmitted diseases using a live Salmonella vaccine

[Invention Disclosure].

By: H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

2014 conference paper

Comparative analysis of 16S amplicon sequencing data from Roche 454 and Ion Torrent PGM platforms

Allali, I., Cadenas, M. B., Ballou, A., Mendoza, M., Ali, R., Hassan, H., … Azcarate-Peril, M. A. (2014, May 20). Presented at the UNC Research Computing Symposium.

By: I. Allali, M. Cadenas, A. Ballou, M. Mendoza, R. Ali, H. Hassan, M. Koci, M. Azcarate-Peril

Event: UNC Research Computing Symposium on May 20, 2014

Source: NC State University Libraries
Added: March 12, 2022

2014 conference paper

Development of the chick microbiome: How early exposure influences future microbial diversity

Presented at the Symposium on Gut Health, St Louis, MO.

By: A. Ballou, R. Ali, M. Mendoza, H. Hassan & M. Koci

Event: Symposium on Gut Health at St Louis, MO

Source: NC State University Libraries
Added: March 12, 2022

2014 journal article

Ferric Uptake Regulator-Dependent Antinitrosative Defenses in Salmonella enterica Serovar Typhimurium Pathogenesis

INFECTION AND IMMUNITY, 82(1), 333–340.

By: M. Husain*, J. Jones-Carson*, L. Liu*, M. Song*, J. Saah*, B. Troxell n, M. Mendoza n, H. Hassan n, A. Vazquez-Torresa

MeSH headings : Analysis of Variance; Animals; Bacterial Proteins / immunology; Bacterial Proteins / physiology; Disease Models, Animal; Mice; Nitric Oxide Synthase Type II; Oxidative Stress / physiology; Repressor Proteins / deficiency; Repressor Proteins / immunology; Repressor Proteins / physiology; Salmonella Infections / microbiology; Salmonella Infections / physiopathology; Salmonella typhimurium / immunology; Salmonella typhimurium / pathogenicity; Stress, Physiological / physiology
TL;DR: Investigations indicate that, in addition to playing a critical role in iron homeostasis, Fur is an important antinitrosative determinant of Salmonella pathogenesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Pyruvate Protects Pathogenic Spirochetes from H2O2 Killing

PLoS ONE, 9(1), e84625.

By: B. Troxell*, J. Zhang*, T. Bourret*, M. Zeng*, J. Blum*, F. Gherardini*, H. Hassan n, X. Yang*

Ed(s): R. Wooten

MeSH headings : Acetates / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Borrelia burgdorferi / drug effects; Borrelia burgdorferi / genetics; Borrelia burgdorferi / metabolism; DNA Repair / drug effects; Gene Deletion; Glucose Oxidase / metabolism; Humans; Hydrogen Peroxide / toxicity; MutS DNA Mismatch-Binding Protein / genetics; MutS DNA Mismatch-Binding Protein / metabolism; Neutrophils / metabolism; Neutrophils / microbiology; Oxidative Stress / drug effects; Pyruvic Acid / metabolism; Pyruvic Acid / pharmacology; Reactive Oxygen Species / metabolism; Spirochaetales / drug effects; Spirochaetales / genetics; Spirochaetales / metabolism
TL;DR: It is demonstrated that pyruvate, in addition to its classical role in central carbon metabolism, serves as an important H2O2 scavenger for pathogenic spirochetes and reduced ROS generated by human neutrophils in response to the Toll-like receptor 2 (TLR2) agonist zymosan. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2014 conference paper

The Chicken Gut Microbiome and Salmonella

Hassan, H. M. (2014, October 21). Invited presentation presented at the United State Animal Health Association – Annual Meeting, Arkansas, MO.

By: H. Hassan

Event: United State Animal Health Association – Annual Meeting at Arkansas, MO on October 21, 2014

Source: NC State University Libraries
Added: March 12, 2022

2013 report

Attenuated FNR deficient enterobacteria

(Patent No. US8435506B2).

By: H. Hassan, R. Fink & M. Evans

Source: NC State University Libraries
Added: March 12, 2022

2013 review

Transcriptional regulation by Ferric Uptake Regulator (Fur) in pathogenic bacteria

[Review of ]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 3.

By: B. Troxell* & H. Hassan n

author keywords: Ferric Uptake Regulator; iron; oxidative stress; gene regulation; pathogenic bacteria
MeSH headings : Animals; Bacteria / genetics; Bacteria / isolation & purification; Bacteria / metabolism; Bacterial Infections / microbiology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Ferric Compounds / metabolism; Gene Expression Regulation, Bacterial; Host-Pathogen Interactions; Humans; Oxidative Stress; Reactive Oxygen Species / metabolism; Reactive Oxygen Species / toxicity; Repressor Proteins / genetics; Repressor Proteins / metabolism; Transcription, Genetic
TL;DR: This review focuses on the breadth of Fur regulation in pathogenic bacteria since mutants of fur, perR, or zur exhibit reduced virulence within numerous animal and plant models of infection. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 report

Attenuated FNR Deficient Enterobacteria

(US Patent No. 8,101,168).

By: H. Hassan, R. Fink & M. Evans

Source: NC State University Libraries
Added: March 12, 2022

2012 journal article

Direct fed microbial supplementation repartitions host energy to the immune system

JOURNAL OF ANIMAL SCIENCE, 90(8), 2639–2651.

By: R. Qiu n, J. Croom n, R. Ali n, A. Ballou n, C. Smith n, C. Ashwell n, H. Hassan n, C. Chiang, M. Koci n

Contributors: R. Qiu n, J. Croom n, R. Ali n, A. Ballou n, C. Smith n, C. Ashwell n, H. Hassan n, C. Chiang, M. Koci n

author keywords: broiler; direct fed microbials; energy metabolism; gene expression; probiotics; systemic immune function
MeSH headings : Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Chickens / immunology; Chickens / metabolism; Diet / veterinary; Dietary Supplements; Energy Metabolism / immunology; Enzyme-Linked Immunosorbent Assay; Erythrocytes / immunology; Gene Expression Regulation / immunology; Gram-Positive Bacteria / physiology; Immunoglobulin A / blood; Immunoglobulin G / blood; Male; Oxygen Consumption; Sheep; Time Factors
TL;DR: Data indicate that supplementation with the direct fed microbial used in this study resulted in energy re-partitioning to the immune system and an increase in antibody production independent of changes in whole body metabolism or growth performance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Analysis of the ArcA regulon in anaerobically grown Salmonella enterica sv. Typhimurium

BMC MICROBIOLOGY, 11.

By: M. Evans n, R. Fink n, A. Vazquez-Torres*, S. Porwollik*, J. Jones-Carson*, M. McClelland*, H. Hassan n

MeSH headings : Anaerobiosis; Animals; Female; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Metabolic Networks and Pathways / genetics; Mice; Mice, Inbred C57BL; Microarray Analysis; Regulon; Repressor Proteins / genetics; Repressor Proteins / metabolism; Salmonella typhimurium / genetics; Salmonella typhimurium / growth & development; Salmonella typhimurium / metabolism
TL;DR: The results demonstrated that in S. Typhimurium, ArcA serves as a transcriptional regulator coordinating cellular metabolism, flagella biosynthesis, and motility, and a set of 120 genes whose regulation was shared with the anaerobic redox regulator, Fnr. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Fur Negatively Regulates hns and Is Required for the Expression of HilA and Virulence in Salmonella enterica Serovar Typhimurium

JOURNAL OF BACTERIOLOGY, 193(2), 497–505.

By: B. Troxell n, M. Sikes n, R. Fink n, A. Vazquez-Torres n, J. Jones-Carson* & H. Hassan n

MeSH headings : Animals; Bacterial Proteins / biosynthesis; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Chromatin Immunoprecipitation; DNA, Bacterial / metabolism; DNA-Binding Proteins / biosynthesis; DNA-Binding Proteins / metabolism; Disease Models, Animal; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes, Bacterial; Genomic Islands; Mice; Mice, Inbred C3H; Operon; Protein Binding; Repressor Proteins / genetics; Repressor Proteins / metabolism; Salmonella Infections, Animal / microbiology; Salmonella typhimurium / pathogenicity; Salmonella typhimurium / physiology; Survival Analysis; Trans-Activators / biosynthesis; Transcription Factors / biosynthesis; Virulence; Virulence Factors / biosynthesis
TL;DR: Fur is required for virulence in Salmonella enterica serovar Typhimurium and that Fur is necessary for the activation of hilA, as well as of other HilA-dependent genes, invF and sipC, and chromatin immunoprecipitation was used to show that Fur bound to the upstream region of hns in a metal-dependent fashion. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

The Fur regulon in anaerobically grown Salmonella enterica sv. Typhimurium: identification of new Fur targets

BMC MICROBIOLOGY, 11.

By: B. Troxell n, R. Fink n, S. Porwollik*, M. McClelland* & H. Hassan n

MeSH headings : Anaerobiosis; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Gene Expression Regulation, Bacterial; Iron / metabolism; Oxygen / metabolism; Regulon; Repressor Proteins / genetics; Repressor Proteins / metabolism; Salmonella typhimurium / genetics; Salmonella typhimurium / metabolism
TL;DR: This work identified twenty-six new targets of Fur regulation, and demonstrates that H-NS repressed genes are down-regulated in Δfur, indicating that Fur is a positive factor in ftnB regulation, while serving to repress the expression of hmpA. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Evolution of the Probiotic Concept: From Conception to Validation and Acceptance in Medical Science

ADVANCES IN APPLIED MICROBIOLOGY, VOL 72, Vol. 72, pp. 1–41.

By: W. Dobrogosz n, T. Peacock* & H. Hassan n

MeSH headings : Animals; Bifidobacterium; Gastrointestinal Tract / microbiology; Humans; Immunologic Factors / pharmacology; Lactobacillus; Organisms, Genetically Modified / microbiology; Probiotics / pharmacology; Probiotics / therapeutic use
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Role of Antioxidant Enzymes in Bacterial Resistance to Organic Acids

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(9), 2747–2753.

By: J. Bruno-Barcena n, M. Azcarate-Peril* & H. Hassan*

Contributors: J. Bruno-Bárcena n, M. Andrea Azcárate-Peril* & H. Hassan*

MeSH headings : Antioxidants / metabolism; Antioxidants / physiology; Bacterial Proteins / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Hydrogen-Ion Concentration; Iron / metabolism; Lactic Acid / toxicity; Oxidative Stress; Streptococcus thermophilus / drug effects; Streptococcus thermophilus / enzymology; Stress, Physiological; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Superoxide Dismutase / physiology
TL;DR: It is suggested that acid stress triggers an iron-mediated oxidative stress that can be ameliorated by MnSOD and iron chelators and may explain the ability of some microorganisms to survive better in acidified environments, as in acid foods during fermentation and accumulation of lactic acid or during passage through the low pH of the stomach. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 journal article

Antimicrobial properties of milkfat globule membrane fractions

JOURNAL OF FOOD PROTECTION, 71(1), 126–133.

By: D. Clare n, Z. Zheng*, H. Hassan n, H. Swaisgood n & G. Catignani n

MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Bacillus cereus / growth & development; Cattle; Consumer Product Safety; Culture Media / chemistry; Escherichia coli O157 / growth & development; Food Contamination / prevention & control; Food Microbiology; Food Preservation / methods; Food Preservatives / pharmacology; Hydrogen Peroxide / pharmacology; Lactobacillus / growth & development; Membrane Proteins / pharmacology; Microbial Sensitivity Tests; Milk / chemistry; Protein Hydrolysates / pharmacology; Pseudomonas fluorescens / growth & development; Salmonella typhimurium / growth & development
TL;DR: It is determined that the antimicrobial effects observed on BHI plates were due to the generation of H2O2 by xanthine oxidase, a major protein constituent of the MFGMs, as a result of purine catalysis, and this substrate is present in BHI but lacking in LB medium. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Effects of pH, Dissolved Oxygen, and Ionic Strength on the Survival of Escherichia coli O157:H7 in Organic Acid Solutions

JOURNAL OF FOOD PROTECTION, 71(12), 2404–2409.

By: A. Kreske n, K. Bjornsdottir n, F. Breidt n & H. Hassan n

MeSH headings : Acids / pharmacology; Colony Count, Microbial; Consumer Product Safety; Cucumis sativus / microbiology; Dose-Response Relationship, Drug; Escherichia coli O157 / drug effects; Escherichia coli O157 / growth & development; Food Contamination / analysis; Food Preservation / methods; Humans; Hydrogen-Ion Concentration; Osmolar Concentration; Oxygen / pharmacology
TL;DR: The results demonstrate that E. coli O157:H7 may survive better than previously expected from studies with acid solutions containing dissolved oxygen, and many acid and acidified foods are sold in hermetically sealed containers with oxygen-limiting conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 293(4), G729–G738.

By: I. Carroll*, J. Andrus*, J. Bruno-Barcena n, T. Klaenhammer n, H. Hassan n & D. Threadgill

Contributors: I. Carroll*, J. Andrus*, J. Bruno-Bárcena n, T. Klaenhammer n, H. Hassan n & D. Threadgill

author keywords: probiotic; Lactobacilli; oxidative stress; inflammatory bowel disease
MeSH headings : Animals; Anti-Inflammatory Agents / therapeutic use; Colitis / drug therapy; Colitis / pathology; Colon / pathology; Cyclooxygenase 2 / metabolism; Disease Models, Animal; Immunohistochemistry; Interleukin-10 / deficiency; Lactobacillus / enzymology; Mice; Peroxidase / metabolism; Probiotics / therapeutic use; Specific Pathogen-Free Organisms; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / therapeutic use
TL;DR: This study demonstrates that L. gasseri producing MnSOD has significant anti-inflammatory activity that reduces the severity of colitis in the IL-10-deficient mouse. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar typhimurium (ATCC 14028s)

JOURNAL OF BACTERIOLOGY, 189(6), 2262–2273.

By: R. Fink n, M. Evans n, S. Porwollik*, A. Vazquez-Torres*, J. Jones-Carson*, B. Troxell n, S. Libby*, M. McClelland*, H. Hassan n

MeSH headings : Anaerobiosis; Animals; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cells, Cultured; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli Proteins / genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Humans; Iron-Sulfur Proteins / genetics; Iron-Sulfur Proteins / metabolism; Macrophages, Peritoneal / microbiology; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Mutation; Oligonucleotide Array Sequence Analysis; Salmonella Infections / microbiology; Salmonella Infections / pathology; Salmonella typhimurium / genetics; Salmonella typhimurium / growth & development; Salmonella typhimurium / metabolism; Salmonella typhimurium / pathogenicity; Transcription Factors / genetics; Transcription Factors / metabolism; Virulence
TL;DR: The role of FNR as a positive regulator of motility, flagellar biosynthesis, and pathogenesis was confirmed by showing that the isogenic fnr mutant is nonmotile, lacks flagella, is attenuated in mice, and does not survive inside macrophages. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(3), 1891–1899.

By: M. Azcarate-Peril n, J. Bruno-Barcena*, H. Hassan* & T. Klaenhammer n

Contributors: M. Azcarate-Peril n, J. Bruno-Bárcena*, H. Hassan* & T. Klaenhammer n

MeSH headings : Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Gene Expression Regulation, Bacterial; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Oligonucleotide Array Sequence Analysis; Operon; Oxalates / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
TL;DR: Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription, andOxalate-dependent induction of these genes occurred only in cells first adapted to subinhibitory concentrations of oxalate and then exposed to pH 5.5. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2005 journal article

Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri

FEMS MICROBIOLOGY LETTERS, 246(1), 91–101.

By: J. Bruno-Barcena n, M. Azcarate-Peril n, T. Klaenhammer n & H. Hassan n

Contributors: J. Bruno-Bárcena n, M. Azcárate-Peril n, T. Klaenhammer n & H. Hassan n

author keywords: functional gene replacement; manganese superoxide dismutase; oxidative stress; Lactobacillus gasseri; lactic acid bacteria; probiotics
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / analysis; Chromosomes, Bacterial / genetics; Gene Expression; Hydrogen Peroxide / pharmacology; Lactobacillus / enzymology; Lactobacillus / genetics; Lactobacillus / growth & development; Plasmids / genetics; RNA, Bacterial / analysis; RNA, Messenger / analysis; Streptococcus thermophilus / enzymology; Streptococcus thermophilus / genetics; Superoxide Dismutase / genetics; Transformation, Bacterial
TL;DR: This is the first report showing the successful utilization of the pORI plasmids system to generate marker-free gene integration in L. gasseri strains. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2004 journal article

Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8), 4702–4710.

By: J. Bruno-Barcena*, J. Andrus*, S. Libby*, T. Klaenhammer n & H. Hassan n

Contributors: J. Bruno-Bárcena*, J. Andrus*, S. Libby*, T. Klaenhammer n & H. Hassan n

MeSH headings : Cloning, Molecular; Humans; Hydrogen Peroxide / pharmacology; Intestines / microbiology; Lactobacillus / classification; Lactobacillus / drug effects; Lactobacillus / enzymology; Lactobacillus / genetics; Oxidative Stress / drug effects; Protein Biosynthesis; Streptococcus / enzymology; Streptococcus / genetics; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Transcription, Genetic
TL;DR: The data show that MnSOD protects cells against hydrogen peroxide by removing O2·− and preventing the redox cycling of iron and is the first report of a sodA from S. thermophilus being expressed in other lactic acid bacteria. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2004 journal article

Role of catalase and oxyR in the viable but nonculturable state of Vibrio vulnificus

FEMS MICROBIOLOGY ECOLOGY, 50(3), 133–142.

By: I. Kong*, T. Bates*, A. Hulsmann*, H. Hassan n, B. Smith* & J. Oliver*

author keywords: VBNC; oxidative stress; catalase; H2O2; nonculturability
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Catalase / metabolism; Cold Temperature; Colony Count, Microbial; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Gene Deletion; Hydrogen Peroxide / toxicity; Microbial Viability; Molecular Sequence Data; Sequence Analysis, DNA; Trans-Activators / genetics; Trans-Activators / metabolism; Vibrio vulnificus / drug effects; Vibrio vulnificus / genetics; Vibrio vulnificus / growth & development; Vibrio vulnificus / physiology
TL;DR: This is the first study providing direct evidence for the metabolic basis of nonculturability and the viable but nonculturable state and an oxyR mutant which lacks catalase activity is generated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Effect of malic acid on the growth kinetics of Lactobacillus plantarum

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 63(2), 207–211.

By: F. Passos*, H. Fleming*, H. Hassan* & R. McFeeters*

MeSH headings : Cucumis sativus / metabolism; Cucumis sativus / microbiology; Culture Media; Fermentation; Kinetics; Lactobacillus / drug effects; Lactobacillus / growth & development; Malates / pharmacology
TL;DR: The results clearly demonstrate the energy-sparing effect of malic acid on the growth- and non-growth-associated energy requirements for L. plantarum. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Molecular characterization and functional analysis of the manganese-containing superoxide dismutase gene (sodA) from Streptococcus thermophilus AO54

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 420(1), 103–113.

By: J. Andrus n, S. Bowen n, T. Klaenhammer n & H. Hassan n

author keywords: Streptococcus thermophilus; superoxide dismutase; MnSOD; sodA sequence; sodA mutant; SOD-null mutant; oxygen sensitivity; manganese ions; DNA polymerase III
MeSH headings : Amino Acid Sequence; Gene Expression Regulation, Enzymologic / drug effects; Magnesium Chloride / pharmacology; Molecular Sequence Data; Mutagenesis, Site-Directed; Sequence Alignment; Sequence Analysis, Protein; Species Specificity; Streptococcus / chemistry; Streptococcus / drug effects; Streptococcus / enzymology; Streptococcus / genetics; Structure-Activity Relationship; Superoxide Dismutase / chemistry; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism
TL;DR: The results suggest that sodA is essential for the aerobic growth of S. thermophilus and in the absence of functional SodA, manganese ions may provide partial protection against oxygen toxicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

RpoS-dependent stress response and exoenzyme production in Vibrio vulnificus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(10), 6114–6120.

By: A. Hulsmann*, T. Rosche*, I. Kong*, H. Hassan n, D. Beam* & J. Oliver*

MeSH headings : Culture Media; Enzymes / biosynthesis; Heat-Shock Response; Humans; Hydrogen Peroxide / pharmacology; Hydrogen-Ion Concentration; Molecular Sequence Data; Movement; Mutation; Osmolar Concentration; Vibrio vulnificus / enzymology; Vibrio vulnificus / genetics; Vibrio vulnificus / growth & development; Vibrio vulnificus / physiology
TL;DR: These studies suggest that rpoS in V. vulnificus is important for adaptation to environmental changes and may have a role in virulence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Azotobacter chroococcum does not contain sodA or its gene product Mn-superoxide dismutase

Canadian Journal of Microbiology, 48(2), 183–187.

By: J. Caldwell n & H. Hassan*

author keywords: FeSOD; Cu-ZnSOD; MnSOD; Azotobacter chroococcum; Azotobacter vinelandii
MeSH headings : Amino Acid Sequence; Azotobacter / enzymology; Azotobacter / genetics; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Homology, Amino Acid; Superoxide Dismutase / analysis; Superoxide Dismutase / genetics
TL;DR: Results dispute the assertion that A. chroococcum contains Mn-superoxide dismutase and refute the assertion by Buchanan and Lees (Can. J. Microbiol. 26: 441-447, 1980). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Characterization of the iron superoxide dismutase gene of <i>Azotobacter vinelandii</i>: <i>sodB</i> may be essential for viability

Canadian Journal of Microbiology, 47(1), 63–71.

By: B. Qurollo*, P. Bishop* & H. Hassan*

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2001 speech

Oxygen Radical Toxicity

Hassan, H. (2001, May). Presented at the Annual Meeting of the Genotoxicity and Environmental Mutagen Society (GEMS), Research Triangle Park, NC.

By: H. Hassan

Event: Annual Meeting of the Genotoxicity and Environmental Mutagen Society (GEMS) at Research Triangle Park, NC on May 16, 2001

Source: NC State University Libraries
Added: March 12, 2022

1998 journal article

Mechanism of regulation of 8-hydroxyguanine endonuclease by oxidative stress: Roles of fnr, arcA, and fur

FREE RADICAL BIOLOGY AND MEDICINE, 24(7-8), 1193–1201.

By: H. Lee*, Y. Lee*, H. Kim*, J. Choi*, H. Hassan n & M. Chung*

author keywords: oh(8)Gua endonuclease; regulation; DNA repair; regulon genes; MutM; FPG; free radical
MeSH headings : Bacterial Outer Membrane Proteins / genetics; Bacterial Proteins / genetics; Base Sequence; Catalase / biosynthesis; Chelating Agents / pharmacology; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; DNA-Formamidopyrimidine Glycosylase; Enzyme Induction / drug effects; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli Proteins; Free Radicals / metabolism; Genes, Bacterial; Glucosephosphate Dehydrogenase / biosynthesis; Hydrogen Peroxide / toxicity; Iron-Sulfur Proteins / genetics; Molecular Sequence Data; Mutation; N-Glycosyl Hydrolases / biosynthesis; Oxidative Stress / drug effects; Paraquat / toxicity; Repressor Proteins / genetics; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / metabolism
TL;DR: Findings suggest that the oxidative regulation of oh8Gua endonuclease is under control of fnr, fur, and arcA, where fnr plays a predominant role, and will enhance the efficiency of cellular growth and survival in the aerobic environment. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1997 journal article

Characterization of superoxide dismutase in Streptococcus thermophilus

Applied and Environmental Microbiology, 63(9), 3732–3735.

By: S. Chang & H. Hassan

Source: NC State University Libraries
Added: August 6, 2018

1997 chapter

Cytotoxicity of oxyradicals and the evolution of superoxide dismutases

In D. Massaro & L. Clerch (Eds.), Oxygen, gene expression and cellular function (pp. 26–51). New York: Marcel Dekker.

By: H. Hassan

Ed(s): D. Massaro & L. Clerch

Source: NC State University Libraries
Added: March 12, 2022

1996 journal article

Enhancement of the antibacterial activity of ampicillin by liposome encapsulation

Drug Delivery, 3(4), 273–278.

By: O. Sammour* & H. Hassan n

TL;DR: It was showed that encapsulation of ampicillin in liposomes prepared with synthetic lecithins enhanced its antibiotic activity against both Amp-sensitive and Amp-resistant Escherichia coli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1995 journal article

Binding of integration host factor (IHF) to the Escherichia coli sodA gene and its role in the regulation of a sodA-lacZ fusion gene

Molecular and General Genetics MGG, 246(2), 228–235.

By: D. Presutti n & H. Hassan n

author keywords: ESCHERICHIA COLI; SUPEROXIDE DISMUTASE (SOD); INTEGRATION HOST FACTOR (IHF); MOBILITY-SHIFT
MeSH headings : 2,2'-Dipyridyl / pharmacology; Aerobiosis; Anaerobiosis; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; DNA, Bacterial / metabolism; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Gene Expression Regulation, Bacterial; Integration Host Factors; Lac Operon; Molecular Sequence Data; Promoter Regions, Genetic; Protein Binding; Recombinant Fusion Proteins / biosynthesis; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / genetics
TL;DR: The clectrophoretic mobility-shift assay was used to reveal specific DNA-protein interactions between DNA fragments containing the sodA promoter and proteins present in Escherichia coli cell-free extracts, and evidence presented suggests that IHF binds to multiple sites in the SodA promoter. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1995 speech

Metals in Biology

Presented at the Keystone Symposium, Park City, UT.

By: H. Hassan

Event: Keystone Symposium at Park City, UT on March 20-24, 1995

Source: NC State University Libraries
Added: March 12, 2022

1994 chapter

Regulation of the biosynthesis of manganese-superoxide dismutase in Escherichia coli in response to iron and manganese

In K. Asada & T. Yoshikawa (Eds.), Frontiers of reactive oxygen species in biology and medicine (pp. 245–250). NY: Elsevier Science.

By: H. Hassan

Ed(s): K. Asada & T. Yoshikawa

Source: NC State University Libraries
Added: March 12, 2022

1994 journal article

Roles of manganese and iron in the regulation of the biosynthesis of manganese-superoxide dismutase inEscherichia coli

FEMS Microbiology Reviews, 14(4), 315–323.

By: H. Hassan* & L. Schrum

MeSH headings : Amino Acid Sequence; Bacterial Proteins / physiology; Base Sequence; Escherichia coli / enzymology; Escherichia coli Proteins; Iron / pharmacology; Iron-Sulfur Proteins; Manganese / pharmacology; Molecular Sequence Data; Repressor Proteins / physiology; Superoxide Dismutase / biosynthesis
TL;DR: A model for the regulation of the MnSOD has been proposed in which the Mn SOD gene (sodA) is negatively regulated at the level of transcription by an iron-containing redox-sensitive repressor protein. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1994 journal article

Stability of Escherichia coli sodA mRNA and identification of the transcriptional start site(s) under different environmental and oxidative stresses

Free Radical Biology and Medicine, 17(3), 209–213.

By: L. Schrum n & H. Hassan n

author keywords: MN-SUPEROXIDE DISMUTASE; SODA MESSENGER-RNA STABILITY; TRANSCRIPTION START SITE; NALIDIXIC ACID; PARAQUAT; 2,2'-DIPYRIDYL; TRANSCRIPTION; TRANSLATION; FREE RADICALS
MeSH headings : 2,2'-Dipyridyl / pharmacology; Base Sequence; Drug Stability; Escherichia coli / genetics; Half-Life; Manganese; Molecular Sequence Data; Nalidixic Acid / pharmacology; Oxidation-Reduction; Oxidative Stress; Paraquat / pharmacology; Promoter Regions, Genetic; RNA, Messenger / metabolism; Reactive Oxygen Species / pharmacology; Superoxide Dismutase / genetics; Transcription, Genetic / drug effects
TL;DR: Results indicate that under oxidative stress/inducing conditions, the increase in aerobic transcription of sodA occurs from only one transcription start site without affecting the stability of SodA mRNA, and suggests that no translational regulation occurs aerobically. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1994 journal article

The Effects of fur on the Transcriptional and Post-transcriptional Regulation of MnSOD Gene (sodA) in Escherichia coli

Archives of Biochemistry and Biophysics, 309(2), 288–292.

By: L. Schrum n & H. Hassan n

MeSH headings : Aerobiosis; Bacterial Proteins / pharmacology; Escherichia coli / enzymology; Escherichia coli / genetics; Gene Expression Regulation, Bacterial / drug effects; Iron / metabolism; Manganese; Mutation; Plasmids; RNA, Messenger / metabolism; Recombinant Fusion Proteins / metabolism; Repressor Proteins / pharmacology; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Transcription, Genetic / drug effects; beta-Galactosidase / genetics
TL;DR: Fur plays the role of a repressor at the transcriptional level, but it also plays an indirect role at the post-transcriptional level where it affects the maturation of SodA into a fully active enzyme, Mn2SodA (MnSOD). (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1993 journal article

Characterization of cis-Acting Regulatory Mutations Causing Anaerobic Expression of the sodA Gene in Escherichia coli

Archives of Biochemistry and Biophysics, 302(2), 372–379.

By: S. Bowen n & H. Hassan n

MeSH headings : Alleles; Anaerobiosis; Base Sequence; DNA Transposable Elements / genetics; DNA, Recombinant / genetics; Escherichia coli / genetics; Gene Expression Regulation, Bacterial / drug effects; Genes, Bacterial / genetics; Molecular Sequence Data; Mutagenesis, Insertional / genetics; Paraquat / pharmacology; Plasmids / genetics; Promoter Regions, Genetic / genetics; Recombinant Proteins / biosynthesis; Regulatory Sequences, Nucleic Acid / genetics; Sequence Homology, Nucleic Acid; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / genetics; beta-Galactosidase / biosynthesis; beta-Galactosidase / genetics
TL;DR: To further investigate the mode of regulation of the gene (sodA) encoding this enzyme, cis-acting regulatory mutations in a strain containing a sodA::lacZ gene fusion were studied and revealed an IS2 insertion element in the promoter region of one of the mutants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1993 journal article

Characterization of regulatory mutations causing anaerobic derepression of the sodA gene in Escherichia coli K12: cooperation between cis- and trans-acting regulatory loci

Journal of General Microbiology, 139(11), 2677–2684.

By: M. Beaumont n & H. Hassan n

MeSH headings : Base Sequence; Escherichia coli / enzymology; Escherichia coli / genetics; Gene Expression Regulation, Bacterial / physiology; Gene Expression Regulation, Enzymologic / physiology; Genes, Bacterial / physiology; Genes, Regulator / physiology; Iron Chelating Agents / metabolism; Lac Operon; Molecular Sequence Data; Mutation / physiology; Regulatory Sequences, Nucleic Acid / physiology; Superoxide Dismutase / genetics; Transcriptional Activation / physiology
TL;DR: The data presented suggest that the cis-mutation in UV14 (sodA14) affects the Fur-binding site in the sodA promoter, while having no effect on Fnr or Arc mediated repression. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1993 journal article

Cloning and expression of the manganese superoxide dismutase gene of Escherichia coli in Lactococcus lactis and Lactobacillus gasseri

Molecular and General Genetics MGG, 239(1-2), 33–40.

By: D. Roy n, T. Klaenhammer n & H. Hassan n

author keywords: ESCHERICHIA-COLI; SUPEROXIDE DISMUTASE; FUSION PROTEIN; LACTOCOCCUS; LACTOBACILLUS
MeSH headings : Base Sequence; Blotting, Western; Cloning, Molecular; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / growth & development; Gene Expression Regulation, Enzymologic / drug effects; Lactobacillus / genetics; Lactococcus lactis / genetics; Manganese / pharmacology; Molecular Sequence Data; Oxygen / metabolism; Paraquat / pharmacology; Plasmids; Superoxide Dismutase / genetics
TL;DR: The Escherichia coli sodA gene encoding the antioxidant enzyme Mn-containing superoxide dismutase (MnSOD), was cloned in the expression vector pMG36e, which has a multiple cloning site down-stream of a promoter and Shine-Dalgarno sequences derived from Lactococcus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1993 journal article

Modeling the cucumber fermentation: Growth ofLactobacillus plantarum

Journal of Industrial Microbiology, 12(3-5), 341–345.

By: F. Passos n, D. Ollis n, H. Fleming n, H. Hassan* & R. Felder n

author keywords: LACTIC ACID BACTERIA; LACTIC ACID; ACETIC ACID; SODIUM CHLORIDE; PH; HYDROGEN ION
TL;DR: The present paper summarizes experimental data and the development of a model for growth of the commercially important bacterium, lactobacillus plantarum, in cucumber juice, which provides an excellent correlation of data for the influence on bacterial growth rate of NaCl, protons (H+), and the neutral, inhibitory forms of acetic acid and the fermentation product, lactic acid. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1993 journal article

Modeling the specific growth rate of Lactobacillus plantarum in cucumber extract

Applied Microbiology and Biotechnology, 40(1).

By: F. Passos n, H. Fleming n, D. Ollis n, H. Hassan n & R. Felder n

Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1993 journal article

Transcriptional activation of Mn-superoxide dismutase gene (sodA) of Escherichia coli by MnCl2

Biochimica Et Biophysica Acta (BBA) - Gene Structure and Expression, 1216(2), 186–190.

By: L. Schrum n & H. Hassan n

author keywords: SUPEROXIDE DISMUTASE; MANGANESE ENZYME; TRANSCRIPTION ACTIVATION; TOPOLOGY; IRON-CONTAINING REPRESSOR PROTEIN; (ESCHERICHIA-COLI)
MeSH headings : Chlorides / pharmacology; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / genetics; Gene Expression; Iron / analysis; Manganese / analysis; Manganese Compounds / pharmacology; Mutation; Superoxide Dismutase / genetics; Transcription, Genetic / drug effects; beta-Galactosidase / genetics
TL;DR: Results show that Mn2+ causes activation of sodA transcription, and it is found that manganese was capable of altering DNA topology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1992 journal article

Characterization oftrans-acting regulatory elements affecting the expression of Mn-superoxide dismutase (sodA) inEscherichia coli

Current Microbiology, 25(3), 135–141.

By: M. Beaumont n & H. Hassan n

TL;DR: Complementation studies revealed that eitherarc (aerobic respiratory control) orfur (ferric uptake regulation) loci independently complemented anaerobic expression of asodA::lacZ protein fusion in one mutant strain (UV16), which exhibited phenotypes typical offur andarc mutants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1992 journal article

Isolation and characterization of an Escherichia coli mutant elevated in manganese-containing superoxide dismutase biosynthesis

Biochem. (Life Sci. Adv.), 11, 23–30.

By: J. Rivers & H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

1992 journal article

Regulatory roles of Fnr, Fur, and Arc in expression of manganese-containing superoxide dismutase in Escherichia coli.

Proceedings of the National Academy of Sciences, 89(8), 3217–3221.

By: H. Hassan n & H. Sun n

MeSH headings : 2,2'-Dipyridyl / pharmacology; Aerobiosis; Anaerobiosis; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Genotype; Iron-Sulfur Proteins; Isoenzymes / biosynthesis; Isoenzymes / genetics; Manganese / metabolism; Molecular Sequence Data; Nitrates / pharmacology; Oligodeoxyribonucleotides; Oxidants / pharmacology; Paraquat / pharmacology; Recombinant Fusion Proteins / biosynthesis; Recombinant Proteins / biosynthesis; Repressor Proteins / genetics; Repressor Proteins / metabolism; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / genetics; Transcription Factors / genetics; Transcription Factors / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism; Viral Regulatory and Accessory Proteins; beta-Galactosidase / biosynthesis; beta-Galactosidase / genetics
TL;DR: It is concluded that Fnr, Arc, and Fur act as anaerobic repressors of sodA and the regulation of SodA by Fur (ferric uptake regulation protein), Arc (aerobic respiratory control), and Fnr is independent of the superoxide response regulon SoxRS. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1992 journal article

Transcriptional regulation of Mn-superoxide dismutase gene (sodA) of Escherichia coli is stimulated by DNA gyrase inhibitors

Archives of Biochemistry and Biophysics, 299(1), 185–192.

By: L. Schrum n & H. Hassan n

MeSH headings : 2,2'-Dipyridyl / pharmacology; Aminocoumarins; Anti-Bacterial Agents / pharmacology; Cloning, Molecular; Coumarins / pharmacology; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / genetics; Gene Expression Regulation, Enzymologic / drug effects; Kinetics; Paraquat / pharmacology; Recombinant Fusion Proteins / metabolism; Sodium Iodide / pharmacology; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Topoisomerase II Inhibitors; Transcription, Genetic / drug effects; beta-Galactosidase / genetics; beta-Galactosidase / metabolism
TL;DR: The results indicate that the effect of DNA gyrase inhibitors is secondary to the cis- and trans-regulatory elements of SodA and suggest that changes in DNA topology may affect transcriptional regulation of sodA. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1991 chapter

The role of oxygen free radicals in biology and evolution

In C. Bryant (Ed.), Metazoan Life Without Oxygen (pp. 19–37). NY: Chapman and Hall.

By: H. Hassan & J. Schiavone

Ed(s): C. Bryant

Source: NC State University Libraries
Added: March 12, 2022

1991 journal article

Use of continuous culture for internal pH determination of lactic acid bacteria

Food Microbiology, 8(2), 137–142.

By: L. McDonald n, H. Hassan n, H. Fleming n & M. Daeschel n

TL;DR: Oxygen, manganese, cell density, external pH, and organic acid production were shown not to influence the internal pH of these bacteria when grown in continuous culture. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1990 journal article

Acid Tolerance of Leuconostoc mesenteroides and Lactobacillus plantarum

Applied and Environmental Microbiology, 56(7), 2120–2124.

By: L. McDonald n, H. Fleming n & H. Hassan n

TL;DR: The comparatively low growth-limiting internal pH and ability to maintain a pH gradient at high organic acid concentration may contribute to the ability of L. plantarum to terminate vegetable fermentations. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1990 chapter

Superoxide dismutases in aerobic organisms

In R. G. Alscher & J. R. Cumming (Eds.), Stress Responses in Plants: Adaptation and Acclimation Mechanisms (pp. 175–199). NY: Wiley-Liss.

By: H. Hassan & J. Scandalios

Ed(s): R. Alscher & J. Cumming

Source: NC State University Libraries
Added: March 12, 2022

1990 journal article

Use of site-directed mutagenesis to identify an upstream regulatory sequence of sodA gene of Escherichia coli K-12.

Proceedings of the National Academy of Sciences, 87(7), 2618–2622.

By: S. Naik n & H. Hassan n

MeSH headings : 2,2'-Dipyridyl / pharmacology; Aerobiosis; Base Sequence; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / genetics; Genes, Bacterial; Molecular Sequence Data; Mutation; Oligonucleotide Probes; Paraquat / pharmacology; Plasmids; Regulatory Sequences, Nucleic Acid; Restriction Mapping; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / genetics
TL;DR: The results indicate that the region within and around the mutated sequence probably plays an important role in sodA regulation and that the mutation disrupts a sequence that interacts with the repressor. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1989 journal article

An electron spin resonance study of oxyradical generation in superoxide dismutase- and catalase-deficient mutants of Escherichia coli K-12

Archives of Biochemistry and Biophysics, 271(2), 323–331.

By: H. Schellhorn, S. Pou*, C. Moody* & H. Hassan n

MeSH headings : Catalase / metabolism; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / genetics; Free Radicals; Hydroxides / metabolism; Hydroxyl Radical; Mutation; NAD / metabolism; NADP / metabolism; Oxygen / metabolism; Pyocyanine / pharmacology; Spin Labels; Superoxide Dismutase / metabolism; Superoxides / metabolism
TL;DR: The response of superoxide dismutase- and catalase-deficient strains of Escherichia coli to redox active compounds was examined by electron spin resonance and levels of radicals formed in response to pyocyanine in situ were extremely low. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1989 chapter

Attenuation of Antioxidant Enzymes in Response to Oxidative Stresses1

In J. C. Somogyi & H. R. Müller (Eds.), Nutritional Impact of Food Processing. 25th Symposium of the Group of European Nutritionists, Reykjavik, September 1987 (pp. 278–287).

By: H. Hassan n

Ed(s): J. Somogyi & H. Müller

Event: 25th Symposium of the Group of European Nutritionists at Reykjavik, Iceland

MeSH headings : Antioxidants / pharmacology; Catalase; Oxidation-Reduction; Oxygen / toxicity; Peroxidases; Superoxide Dismutase
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1989 journal article

Effect of temperature andhtpRon the biosynthesis of superoxide dismutase inEscherichia coli

FEMS Microbiology Letters, 58(2-3), 133–137.

By: H. Hassan n & F. Lee*

TL;DR: The synthesis of Mn- and FeSODs in response to temperature changes was examined in strains of Escherichia coli with different mutations in sod and htpR genes, with results that showed FeS OD was more stable at 42 degrees C than was MnSOD. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1989 chapter

Microbial Superoxide Dismutases

In Advances in Genetics (pp. 65–97).

By: H. Hassan n

MeSH headings : Bacteria / enzymology; Bacteria / genetics; Biological Evolution; Cloning, Molecular; Gene Expression Regulation; Manganese / metabolism; Mutation; Plants / enzymology; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Superoxides / metabolism
TL;DR: The evolution of three types of SODs to accomplish the same reaction in different organisms attests to their vital role in aerobic survival and the knowledge gained from E. coli will make it possible to explore the regulation of S ODs in other organisms. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

An assay for the detection of superoxide dismutase in individual Escherichia coli colonies

Analytical Biochemistry, 168(2), 455–461.

By: J. Schiavone n & H. Hassan n

MeSH headings : Bacteriological Techniques; Escherichia coli / enzymology; Mutation; Superoxide Dismutase / analysis
TL;DR: This assay provides a method for screening a population of cells for mutants deficient in or overproducing superoxide dismutase, and can detect the presence of superoxide Dismutase and the relative amount of enzyme as well. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Antibacterial activity of plantaricin SIK-83, a bacteriocin produced by Lactobacillus plantarum

Biochimie, 70(3), 381–390.

By: R. Andersson*, M. Daeschel n & H. Hassan n

MeSH headings : Adsorption; Bacteria / drug effects; Bacteriocins / biosynthesis; Bacteriocins / pharmacology; Bacteriolysis; Cell Membrane / drug effects; Cell Survival / drug effects; Lactobacillus / metabolism; Microbial Sensitivity Tests
TL;DR: It was shown that the bacteriocin binds specifically to sensitive cells but not to nonsensitive lactic acid bacteria, the producer strain or Gram-negative bacteria, and could be rescued by treatment with proteolytic enzymes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Induction of the manganese-containing superoxide dismutase in Escherichia coli is independent of the oxidative stress (oxyR-controlled) regulon.

Journal of Biological Chemistry, 263(29), 14808–14811.

By: S. Bowen n & H. Hassan n

TL;DR: The data clearly demonstrate that the induction of manganese-superoxide dismutase is independent of the oxyR-controlled regulon. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Isolation and characterization of respiratory-deficient mutants of Escherichia coli K-12

Journal of Bacteriology, 170(1), 78–83.

By: H. Schellhorn n & H. Hassan n

MeSH headings : Aerobiosis; Catalase / metabolism; Chromosome Mapping; Culture Media; Cytochromes / metabolism; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / growth & development; Escherichia coli / metabolism; Fermentation; Genes, Bacterial; Glucose / metabolism; Heme / biosynthesis; Kinetics; Mutation; Oxygen Consumption; Peroxidases / metabolism; Superoxide Dismutase / metabolism
TL;DR: Several mutants of Escherichia coli K-12 defective in aerobic metabolism were isolated and one such mutant was found to be deficient in cytochromes, heme, and catalase, which produced lactic acid as the principal fermentation product. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Regulation and synthesis of superoxide dismutase

Free Radical Biology and Medicine, 5, 377–385.

By: H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

1988 journal article

Response of hydroperoxidase and superoxide dismutase deficient mutants of Escherichia coli K-12 to oxidative stress

Canadian Journal of Microbiology, 34(10), 1171–1176.

By: H. Schellhorn & H. Hassan*

MeSH headings : Anaerobiosis; Antioxidants; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / growth & development; Ethylmaleimide / toxicity; Iodoacetates / toxicity; Iodoacetic Acid; Mutation; Oxygen Consumption / drug effects; Peroxidase / deficiency; Peroxidase / genetics; Superoxide Dismutase / deficiency; Superoxide Dismutase / genetics
TL;DR: Strips of E. coli deficient in superoxide dismutase and (or) hydroperoxidase were constructed by generalized transduction and their physiological responses to oxygen and oxidant stress examined, and both types of enzymes were required for an effective defense against chemical oxidants that generate superoxide radicals and hydrogen peroxide. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Role of oxyradicals in the inactivation of catalase by ozone

Free Radical Biology and Medicine, 5(5-6), 305–312.

By: C. Whiteside n & H. Hassan n

MeSH headings : Animals; Antioxidants / pharmacology; Buffers; Catalase / metabolism; Cattle; Enzyme Activation / drug effects; Free Radicals; Hydrogen-Ion Concentration; Ozone / pharmacology; Spectrophotometry; Water
TL;DR: The data suggest the possible involvement of superoxide radicals in the inactivation of catalase by ozone and that SOD is less effective due to its inactivation by ozone. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Stability and expression of a plasmid-containing killer toxin cDNA in batch and chemostat cultures ofsaccharomyces cerevisiae

Biotechnology and Bioengineering, 31(8), 783–789.

By: F. Lee n & H. Hassan n

TL;DR: The effects of dilution rate, D(h−1) on plasmid stability and toxin expression were studied in transformed AH22 (AH22/T3) and Montrachet 522 (522/T1) wine yeast grown in glucose‐limited chemostat cultures and it was suggested that plasmids stability was dependent on the presence of the 2μ plasmide which is naturally present in some yeasts. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 conference paper

Superoxide dismutase: an antioxidant defense enzyme

UCLA Symposia on Molecular and Cellular Biology, 82, 183–193.

By: H. Hassan & H. Schellhorn

Source: NC State University Libraries
Added: March 12, 2022

1988 journal article

The role of redox in the regulation of manganese-containing superoxide dismutase biosynthesis in Escherichia coli.

Journal of Biological Chemistry, 263(9), 4269–4273.

By: J. Schiavone n & H. Hassan n

TL;DR: It is concluded that the redox state of the cell possibly influences the biosynthesis and/or activity of an iron-containing repressor protein that serves to negatively regulate manganese-superoxide dismutase biosynthesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1988 journal article

Transcriptional regulation of katE in Escherichia coli K-12

Journal of Bacteriology, 170(9), 4286–4292.

By: H. Schellhorn n & H. Hassan n

MeSH headings : Aerobiosis; Anaerobiosis; Catalase / biosynthesis; Catalase / genetics; Cloning, Molecular; Enzyme Induction; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli / growth & development; Gene Expression Regulation; Genes, Bacterial; Hydrogen Peroxide / pharmacology; Mutation; Oxidation-Reduction; Transcription, Genetic; Transduction, Genetic
TL;DR: Escherichia coli produces two distinct species of catalase, hydroperoxidases I and II, which differ in kinetic properties and regulation, and katE was fully expressed in air and was not further induced when the growth medium was sparged with 100% oxygen. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1987 journal article

Biosynthesis of superoxide dismutase and catalase in chemostat culture of Saccharomyces cerevisiae

Applied Microbiology and Biotechnology, 26(6), 531–536.

By: F. Lee n & H. Hassan n

TL;DR: The data clearly show that synthesis of SOD and catalase, though correlated with changes in oxygen tension, are independent of one another. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1987 journal article

Biosynthesis of superoxide dismutase in eight prokaryotes: Effects of oxygen, paraquat and an iron chelator

FEMS Microbiology Letters, 42(1), 33–38.

By: J. Schiavone n & H. Hassan n

TL;DR: The data indicate that the induction of S OD in P. vulgaris, E. cloacae, and Salmonella typhimurium is similar to that found in E. coli and suggest that the mechanism of SOD regulation inE. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1987 journal article

Effect of oxygen tension on stability and expression of a killer toxin chimeric plasmid in a chemostat culture of Saccharomyces cerevisiae

Applied Microbiology and Biotechnology, 27(1), 72–74.

By: F. Lee n & H. Hassan n

TL;DR: The effects of oxygen tension and dilution rate on expression and stability of killer toxin chimeric plasmid (pADH-10A) in transformants of wine yeast (Montrachet 522) were investigated using a glucose-limited chemostat culture. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1987 speech

Free Radicals in Health and Disease

Hassan, H. (1987, September). Workshop presented at the Symposium on Nutritional Impact of Food Processing, Reykjavik, Iceland.

By: H. Hassan

Event: Symposium on Nutritional Impact of Food Processing at Reykjavik, Iceland on September 2-4, 1987

Source: NC State University Libraries
Added: March 12, 2022

1987 journal article

Induction and inactivation of catalase and superoxide dismutase of Escherichia coli by ozone

Archives of Biochemistry and Biophysics, 257(2), 464–471.

By: C. Whiteside n & H. Hassan n

MeSH headings : Catalase / antagonists & inhibitors; Catalase / biosynthesis; Enzyme Induction; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / growth & development; Kinetics; Ozone / pharmacology; Superoxide Dismutase / antagonists & inhibitors; Superoxide Dismutase / biosynthesis
TL;DR: Ozonation of cell-free extracts showed that superoxide dismutase and catalase are subject to oxidative inactivation by ozone, suggesting in vivo induction of these enzymes may represent an adaptive response evolved to protect cells against ozone toxicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1987 journal article

Regulation of manganese-containing superoxide dismutase in Escherichia coli. Anaerobic induction by nitrate.

Journal of Biological Chemistry, 262(35), 17173–17177.

By: H. Hassan n & C. Moody n

TL;DR: The data support the previously proposed model for a negatively controlled operon where the repressor molecule is envisioned as an allosteric redox sensing protein and conditions known to oxidize or to deplete the iron are also found to cause induction of the manganese-superoxide dismutase albeit the absence of dioxygen. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1986 chapter

An alternate defense mechanism against paraquat toxicity in Escherichia coli

In G. Rotilio (Ed.), Superoxide and Superoxide Dismutase in Chemistry, Biology and Medicine (pp. 354–357). Elsevier Science.

By: H. Hassan & S. Kao

Ed(s): G. Rotilio

Source: NC State University Libraries
Added: March 12, 2022

1986 journal article

Biosynthesis of superoxide dismutase and catalase inSaccharomyces cerevisiae: effects of oxygen and cytochromec deficiency

Journal of Industrial Microbiology, 1(3), 187–193.

By: F. Lee n & H. Hassan n

TL;DR: Two strains ofSaccharomyces cerevisiae were used to study the synthesis of superoxide dismutase, and the cytochromec-deficient mutant had lower specific growth rate, growth yield, and oxygen uptake than the wild type. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1986 journal article

Effects of cysteine on growth, protease production, and catalase activity of Pseudomonas fluorescens

Applied and Environmental Microbiology, 51(2), 418–421.

By: B. Himelbloom & H. Hassan*

MeSH headings : Catalase / metabolism; Cysteine / pharmacology; Hydrogen Peroxide / pharmacology; Isoenzymes / metabolism; Kinetics; Peptide Hydrolases / biosynthesis; Pseudomonas fluorescens / drug effects; Pseudomonas fluorescens / enzymology; Pseudomonas fluorescens / growth & development
TL;DR: Cysteine inhibits growth of and protease production by Pseudomonas fluorescens NC3 and suggests a role for the endogenous generation of hydrogen peroxide via the autoxidation of cysteine. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1986 chapter

Effects of cytochrome c deficiency, paraquat and copper on the biosynthesis of copper zinc superoxide dismutase and catalase in Saccharomyces cerevisiae

In G. Rotilio (Ed.), Superoxide and Superoxide Dismutase in Chemistry, Biology and Medicine (pp. 300–303). Elsevier Science.

By: H. Hassan & F. Lee

Ed(s): G. Rotilio

Source: NC State University Libraries
Added: March 12, 2022

1986 chapter

Regulation of the synthesis of superoxide dismutase in procaryotes

In G. Rotilo (Ed.), Superoxide and Superoxide Dismutase in Chemistry, Biology and Medicine (pp. 274–279). Elsevier Science.

By: H. Hassan & C. Moody

Ed(s): G. Rotilo

Source: NC State University Libraries
Added: March 12, 2022

1986 conference paper

Role of Superoxide Dismutase in Cellular Protection

Presented at the Frontiers in Basic Sciences that Relate to Heart, Lung, and Blood Diseases Symposium, Bethesda, MD.

By: H. Hassan

Event: Frontiers in Basic Sciences that Relate to Heart, Lung, and Blood Diseases Symposium at Bethesda, MD on December 10-12, 1986

Source: NC State University Libraries
Added: March 12, 2022

1985 journal article

Biochemical characterization of a paraquat-tolerant mutant of Escherichia coli.

Journal of Biological Chemistry, 260(19), 10478–10481.

By: S. Kao n & H. Hassan n

Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1985 journal article

Biosynthesis of superoxide dismutase in Sacchromyces cerevisiae: Effects of paraquat and copper

Journal of Free Radicals in Biology & Medicine, 1(4), 319–325.

By: F. Lee n & H. Hassan n

MeSH headings : Catalase / biosynthesis; Copper / pharmacology; Free Radicals; Kinetics; Oxygen Consumption / drug effects; Paraquat / pharmacology; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / metabolism; Superoxide Dismutase / biosynthesis; Superoxides / metabolism
TL;DR: A cytochrome c deficient mutant strain was found to be more responsive than the wild type strain to paraquat and/or copper by increasing its copper-zinc superoxide dismutase. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1985 journal article

Isolation of paraquat-resistant mutants ofEscherichia coli: lack of correlation between resistance and the activity of superoxide dismutase

FEMS Microbiology Letters, 28(1), 93–97.

By: S. Kao n & H. Hassan n

TL;DR: Resistance to paraquat in these mutant strains is, therefore, unrelated to their capacity to detoxify superoxide or hydrogen peroxide. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1985 chapter

Manipulation of Superoxide Dismutase Levels in Procaryotes

In R. Greenwald (Ed.), CRC Handbook of Methods for Oxygen Radical Research (pp. 353–358). Florida, US: CRC Press Inc.

By: H. Hassan

Ed(s): R. Greenwald

Source: NC State University Libraries
Added: March 12, 2022

1985 journal article

Optimization of the hide powder azure assay for quantitating the protease of Pseudomonas fluorescens

Journal of Microbiological Methods, 4(2), 59–66.

By: B. Himelbloom n & H. Hassan n

TL;DR: Protease production by P. fluorescens NC 3 was initiated during late-logarithmic-growth phase in a sodium caseinate medium and reached its maximum at the onset of the stationary phase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1985 journal article

Regulation of the manganese-containing superoxide dismutase is independent of the inducible DNA repair system in Escherichia coli.

Journal of Biological Chemistry, 260(24), 12954–12956.

By: L. Hancock n & H. Hassan n

TL;DR: Studies on the induction of the manganese-containing superoxide dismutase in several strains of Escherichia coli with different mutations in recA and lexA revealed that the inductions of the Mn-isozyme and of the SOS system by oxygen free radicals are not coregulated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1984 journal article

Anaerobic biosynthesis of the manganese containing superoxide dismutase in Escherichia coli

Journal of Biological Chemistry, 259(20), 12821–12825.

By: C. Moody n & H. Hassan n

TL;DR: A model is presented to account for the observed effects of oxygen, superoxide anion, and chelators on the increased synthesis of MnS OD and the regulatory repressor for MnSOD biosynthesis is envisioned as being an iron-containing protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: March 12, 2022

1984 chapter

Chemistry and Biochemistry of Oxygen and of Its Partially Reduced Derivatives

In J. R. Totter, S. F. Gottlieb, & I. S. Longmuir (Eds.), Oxygen: An In Depth Study of Its Pathophysiology (pp. 307–340). Undersea Medical Society, Inc.

By: H. Hassan

Ed(s): J. Totter, S. Gottlieb & I. Longmuir

Source: NC State University Libraries
Added: March 12, 2022

1984 journal article

Induction of the manganese-containing superoxide dismutase inEscherichia coliby nalidixic acid and by iron chelators

FEMS Microbiology Letters, 25(2-3), 233–236.

By: H. Hassan n & C. Moody n

TL;DR: 2,2′-dipyridyl and 1,10-phenanthroline were shown to cause a 7- to 20-fold increase in the MnSOD of E. coli, and it is proposed that the repressor for MnS OD is an iron-containing protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1984 chapter

Oxygen toxicity in prokaryotes

In J. V. Bannister (Ed.), The Biology and Chemistry of Active Oxygen (pp. 128–138). North Holland: Elsevier.

By: H. Hassan & I. Fridovich

Ed(s): J. Bannister

Source: NC State University Libraries
Added: March 12, 2022

1984 chapter

Superoxide Dismutase: An Antioxidant Defense Enzyme. In Free Radicals in Molecular Biology, Aging and Disease

In D. Armstrong, R. S. Sohal, R. G. Cutler, & T. F. Slater (Eds.), Free Radicals in Molecular Biology, Aging and Disease (pp. 77–85). NY: Raven Press.

By: H. Hassan

Ed(s): D. Armstrong, R. Sohal, R. Cutler & T. Slater

Source: NC State University Libraries
Added: March 12, 2022

1984 journal article

Superoxide dismutase, catalase and peroxidase in four strains of Neisseria meningitidis of different virulence

FEMS Microbiology Letters, 25(1), 71–74.

By: H. Hassan n, A. Bhatti & L. White

TL;DR: It was shown that the presence of catalase could yield an artifactual SOD-band when the SOD activity stain is performed in the presenceof 10 mM H2O2, normally required to inactivate and identify FeSODs. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1984 chapter

[30] Determination of the mutagenicity of oxygen free radicals using microbial systems

In L. Packer (Ed.), Oxygen Radicals in Biological Systems (pp. 254–263).

By: H. Hassan* & C. Moody

Ed(s): L. Packer

MeSH headings : Animals; Bacillus subtilis / drug effects; Biotransformation; Escherichia coli / drug effects; Free Radicals; Hydrogen Peroxide / toxicity; Hydroxides / toxicity; Hydroxyl Radical; Microsomes, Liver / metabolism; Mutagenicity Tests; Mutagens; Mutation; Saccharomyces cerevisiae / drug effects; Salmonella typhimurium / drug effects; Species Specificity; Superoxides / toxicity
TL;DR: This chapter presents methods for assessing the mutagenicity of oxygen free radicals in microbial systems, usually rapid, simple, and inexpensive. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1984 chapter

[53] Determination of microbial damage caused by oxygen free radicals, and the protective role of superoxide dismutase

In L. Packer (Ed.), Oxygen Radicals in Biological Systems (pp. 404–412).

By: H. Hassan*

Ed(s): L. Packer

MeSH headings : Anaerobiosis; Animals; Anti-Bacterial Agents; Electron Transport; Enzyme Induction; Escherichia coli / drug effects; Escherichia coli / metabolism; Humans; Neutrophils / physiology; Oxygen / toxicity; Photolysis; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / metabolism; Superoxides / metabolism; Superoxides / toxicity; Xanthine Oxidase / metabolism
TL;DR: This chapter presents methods for generating oxygen free radicals, for manipulating the cellular concentration of SOD, and for assessing the damage caused by oxygenfree radicals. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1984 chapter

[69] Exacerbation of superoxide radical formation by Paraquat

In L. Packer (Ed.), Oxygen Radicals in Biological Systems (pp. 523–532).

By: H. Hassan*

Ed(s): L. Packer

MeSH headings : Animals; Cyanides / pharmacology; Escherichia coli / drug effects; Escherichia coli / metabolism; Free Radicals; Kinetics; NADP / metabolism; Oxidation-Reduction; Oxygen / metabolism; Oxygen Consumption / drug effects; Paraquat / radiation effects; Paraquat / toxicity; Pulse Radiolysis / methods; Superoxide Dismutase / metabolism; Superoxides / metabolism
TL;DR: This chapter presents methods for measuring and identifying the partially reduced oxygen species generated during the reaction of PQ· + and O 2 . (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1983 speech

Antioxidant Enzymes and Aging

Hassan, H. (1983, October). Lecture presented at the 13th Annual Meeting of the American Aging Association, Washington, DC.

By: H. Hassan

Event: 13th Annual Meeting of the American Aging Association at Washington, DC on October 6-8, 1983

Source: NC State University Libraries
Added: March 12, 2022

1983 speech

Oxygen

Hassan, H. (1983, June). Workshop presented at the Undersea Medical Society and the Institute of Energy Analysis Oak Ridge Associated Universities, Oak Ridge, TN.

By: H. Hassan

Event: Undersea Medical Society and the Institute of Energy Analysis Oak Ridge Associated Universities at Oak Ridge, TN on June 30 - July 1, 1983

Source: NC State University Libraries
Added: March 12, 2022

1983 chapter

Oxygen toxicity and mutagenicity in procaryotes

In G. Cohen & R. Greenwald (Eds.), Oxy Radicals and Their scavengers Systems: Molecular Aspects (Vol. 1, pp. 198–206). Holland: Elsevier.

By: H. Hassan

Ed(s): G. Cohen & R. Greenwald

Source: NC State University Libraries
Added: March 12, 2022

1982 journal article

Biosynthesis of oxygen-detoxifying enzymes in Bdellovibrio stolpii

Journal of Bacteriology, 152(2), 792–796.

By: R. Von Stein, L. Barber & H. Hassan*

MeSH headings : Aerobiosis; Bdellovibrio / enzymology; Catalase / biosynthesis; Isoenzymes / biosynthesis; Kinetics; Oxygen / metabolism; Oxygen Consumption; Peroxidase; Peroxidases / biosynthesis; Superoxide Dismutase / biosynthesis
TL;DR: Induction of superoxide dismutase with 100% oxygen was manifested as an increase in the intensities of the two minor bands of activity, suggesting that isozyme I is constitutive, whereas isozymes II and III are inducible. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1982 chapter

Exacerbations of Oxygen Toxicity by Redox Active Compounds

In T. E. King, H. S. Mason, & M. Morrison (Eds.), Oxidases and Related Redox Systems (pp. 151–167).

By: H. Hassan* & I. Fridovich*

Ed(s): T. King, H. Mason & M. Morrison

Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1982 journal article

MUTAGENICITY OF OXYGEN FREE-RADICALS

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 79(9), 2855–2859.

By: C. Moody & H. Hassan*

MeSH headings : Catalase / metabolism; Free Radicals; Mutagenicity Tests; Mutagens; Oxygen; Paraquat / toxicity; Salmonella typhimurium / drug effects; Superoxide Dismutase / metabolism
TL;DR: The mutagenicity of paraquat appears to be due to its ability to exacerbate the intracellular production of superoxide radicals. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1982 journal article

Superoxide dismutase protects against paraquat-mediated dioxygen toxicity and mutagenicity: studies in Salmonella typhimurium

Canadian Journal of Physiology and Pharmacology, 60(11), 1367–1373.

By: H. Hassan* & C. Moody

MeSH headings : Animals; Electron Transport; Enzyme Induction; Free Radicals; Humans; Mutagens / antagonists & inhibitors; Oxygen / antagonists & inhibitors; Oxygen / metabolism; Oxygen / toxicity; Paraquat / antagonists & inhibitors; Paraquat / toxicity; Salmonella typhimurium / drug effects; Salmonella typhimurium / genetics; Superoxide Dismutase / metabolism; Superoxide Dismutase / pharmacology
TL;DR: Cells containing high levels of superoxide dismutase were more resistant to the toxic and mutagenic effects of paraquat than were cells containing a normal level of this enzyme. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1981 journal article

Chemistry and biochemistry of superoxide dismutases

European Journal of Rheumatology and Inflammation, 4, 160–172.

By: H. Hassan & I. Fridovich

Source: NC State University Libraries
Added: March 12, 2022

1981 chapter

Superoxide and superoxide dismutases in Escherichia coli: threat and defense

In K. Bloch, L. Bolis, D. C. Tosteson, & J. Wright (Eds.), Membranes, Molecules, Toxins, and Cells (pp. 123–133). Boston: PSG Inc.

By: H. Hassan

Ed(s): K. Bloch, L. Bolis, D. Tosteson & J. Wright

Source: NC State University Libraries
Added: March 12, 2022

1981 report

Vacuum Drum Planters

(Patent No. US4306509A).

By: A. Hassan & H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

1980 chapter

Impermeability of the Escherichia coli cell envelope to superoxide radical

In W. H. Bannister & J. V. Bannister (Eds.), Developments in Biochemistry: Biological and Clinical Aspects of Superoxide and Superoxide Dismutase (Vol. 11B, pp. 57–71). North Holland: Elsevier North Holland.

By: H. Hassan & I. Fridovich

Ed(s): W. Bannister & J. Bannister

Source: NC State University Libraries
Added: March 12, 2022

1980 journal article

Inhibitors of superoxide dismutases: A cautionary tale

Archives of Biochemistry and Biophysics, 199(2), 349–354.

By: H. Hassan*, H. Dougherty* & I. Fridovich*

MeSH headings : Animals; Cattle; Cytochrome c Group / metabolism; Dianisidine / metabolism; Enterococcus faecalis / enzymology; Erythrocytes / enzymology; Escherichia coli / enzymology; Metalloproteins / metabolism; Naphthols / pharmacology; Oxidation-Reduction; Superoxide Dismutase / antagonists & inhibitors; Superoxide Dismutase / blood
TL;DR: It was shown that pamoic acid did not at all inhibit superoxide dismutase but rather diminished the sensitivity of the assays, and the mechanism proposed to account for this effect involved oxidation of pamoate, by O2−, to yield a pamoates radical which can then reduce cytochrome c or oxidize pyrogallol. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1980 journal article

Mechanism of the antibiotic action pyocyanine

Journal of Bacteriology, 141(1), 156–163.

By: H. Hassan* & I. Fridovich

MeSH headings : Catalase / biosynthesis; Enzyme Induction; Escherichia coli / drug effects; Escherichia coli / metabolism; Hydrogen Peroxide / metabolism; Isoenzymes; Oxygen / metabolism; Oxygen Consumption / drug effects; Phenazines / pharmacology; Pseudomonas aeruginosa / drug effects; Pyocyanine / pharmacology; Superoxide Dismutase / biosynthesis; Superoxides / metabolism
TL;DR: It is concluded that pyocyanine diverts electron flow and thus increases the production of O2- and H2O2 and that the antibiotic action of this pigment is largely a reflection of the toxicity of these products of oxygen reduction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1980 chapter

Superoxide Dismutases

In D. Evered & G. Lawrenson (Eds.), Biological Roles of Copper (pp. 125–142).

By: H. Hassan*

Ed(s): D. Evered & G. Lawrenson

MeSH headings : Aerobiosis; Animals; Copper / analysis; Enzyme Induction; Escherichia coli / enzymology; Iron / analysis; Macromolecular Substances; Manganese / analysis; Protein Conformation; Species Specificity; Superoxide Dismutase / metabolism; Superoxides / pharmacology; Zinc / analysis
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1980 chapter

Superoxide Dismutases: Detoxication of a Free Radical

In W. B. Jakoby (Ed.), Enzymatic Basis of Detoxication (pp. 311–332).

By: H. Hassan & I. Fridovich

Ed(s): W. Jakoby

Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1980 speech

Superoxide and Superoxide Dismutases

Hassan, H. (1980, June). Mini symposium presented at the American Society of Biological Chemists Meeting, New Orleans, LA.

By: H. Hassan

Event: American Society of Biological Chemists Meeting at New Orleans, LA on June 4, 1980

Source: NC State University Libraries
Added: March 12, 2022

1979 speech

Clinical Application of Superoxide Dismutase

Hassan, H. (1979, October). Presented at the Chemie Grunenthal GMBH, Aachen, West Germany.

By: H. Hassan

Event: Chemie Grunenthal GMBH at Aachen, West Germany on October 19-20, 1979

Source: NC State University Libraries
Added: March 12, 2022

1979 journal article

Intracellular production of superoxide radical and of hydrogen peroxide by redox active compounds

Archives of Biochemistry and Biophysics, 196(2), 385–395.

By: H. Hassan* & I. Fridovich*

MeSH headings : Catalase / metabolism; Cyanides / pharmacology; Electron Transport; Escherichia coli / drug effects; Escherichia coli / metabolism; Hydrogen Peroxide / metabolism; NAD / metabolism; Oxidation-Reduction; Oxygen / metabolism; Oxygen Consumption / drug effects; Subcellular Fractions / metabolism; Superoxide Dismutase / metabolism; Superoxides / metabolism
TL;DR: The effect of alloxan appeared paradoxical in that it increased cyanide-resistant respiration without significantly increasing the cell content of the manganese-superoxide dismutase and with only a small effect on the level of catalase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1979 chapter

Oxygen toxicity

In M. Shilo (Ed.), Strategy of Microbial Life in Extreme Environments (pp. 223–241). NY: Dahlem Konferenzen, Verlag Chemie.

By: H. Hassan, N. Krinsky, J. Morris, N. Pfenning, H. Schlegel, M. Shilo, G. Vogels, U. Weser, R. Wolfe, J. Kuenen

Ed(s): M. Shilo

Source: NC State University Libraries
Added: March 12, 2022

1979 journal article

Paraquat and Escherichia coli. Mechanism of production of extracellular superoxide radical.

Journal of Biological Chemistry, 254(21), 10846–10852.

By: H. Hassan* & I. Fridovich*

Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1979 journal article

Paraquat and the exacerbation of oxygen toxicity

Trends in Biochemical Sciences, 4(5), 113–115.

By: I. Fridovich* & H. Hassan*

Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1979 chapter

Superoxide dismutase and its role for survival in the presence of oxygen

In M. Shilo (Ed.), Strategy of Microbial Life in Extreme Environments (pp. 179 193). NY: DahlemKonferenzen, Verlag Chemie [Wiley-VCH].

By: H. Hassan & I. Fridovich

Ed(s): M. Shilo

Source: NC State University Libraries
Added: March 12, 2022

1979 journal article

Superoxide, Hydrogen Peroxide, and Oxygen Tolerance of Oxygen-Sensitive Mutants of Escherichia coli

Clinical Infectious Diseases, 1(2), 357–369.

By: H. Hassan* & I. Fridovich*

MeSH headings : Escherichia coli / classification; Escherichia coli / drug effects; Escherichia coli / genetics; Hydrogen Peroxide / toxicity; Mutation; Oxygen / toxicity; Superoxide Dismutase / physiology; Superoxides / toxicity; Virulence
TL;DR: The results indicated that enzymic scavenging of both H2O2 and O2- provides an important defense against oxygen toxicity. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1978 journal article

Regulation and Role of Superoxide Dismutase

Biochemical Society Transactions, 6(2), 356–361.

By: H. Hassan* & I. Fridovich*

MeSH headings : Enzyme Induction; Escherichia coli / metabolism; Glucose / metabolism; Kinetics; Oxygen; Superoxide Dismutase / metabolism
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1978 journal article

Regulation of the synthesis of catalase and peroxidase in Escherichia coli.

Journal of Biological Chemistry, 253(18), 6445–6450.

By: H. Hassan* & I. Fridovich*

Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1978 speech

Strategies of Microbial Life in Extreme Environment

Hassan, H. (1978, November). Workshop presented at the Dahlem Konferenzen, Berlin, W. Germany.

By: H. Hassan

Event: Dahlem Konferenzen at Berlin, W. Germany on November 20-24, 1978

Source: NC State University Libraries
Added: March 12, 2022

1978 journal article

Superoxide radical and the oxygen enhancement of the toxicity of paraquat in Escherichia coli.

Journal of Biological Chemistry, 253(22), 8143–8148.

By: H. Hassan* & I. Fridovich*

Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1977 journal article

Biochemical and physiological properties of alkaline phosphatases in five isolates of marine bacteria

Journal of Bacteriology, 129(3), 1607–1612.

By: H. Hassan* & D. Pratt

MeSH headings : Alkaline Phosphatase / biosynthesis; Alkaline Phosphatase / metabolism; Bacteria / enzymology; Enzyme Repression; Isoenzymes / biosynthesis; Kinetics; Magnesium / pharmacology; Phosphates / pharmacology; Seawater; Species Specificity
TL;DR: The alkaline phosphatase activities of five unique isolates of marine bacteria were found to be associated with the periplasmic space; however, the enzymes from these isolates differed with respect to their repressibility, the apparent number of isoenzymes, the necessity for Mg2 for activity, and the conditions required for their release. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1977 journal article

Enzymatic defenses against the toxicity of oxygen and of streptonigrin in Escherichia coli

Journal of Bacteriology, 129(3), 1574–1583.

By: H. Hassan* & I. Fridovich

MeSH headings : Anaerobiosis; Catalase / biosynthesis; Chloramphenicol / pharmacology; Enzyme Induction / drug effects; Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / ultrastructure; Isoenzymes / biosynthesis; Oxygen / toxicity; Peroxidases / biosynthesis; Puromycin / pharmacology; Streptonigrin / toxicity; Superoxide Dismutase / biosynthesis
TL;DR: Anaerobically grown Escherichia coli K-12 cells showed a much greater oxygen enhancement of the lethality of streptonigrin than did aerobically growing cells, in accord with the proposal that streptonigratedin can serve as an intracellular source of superoxide. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1977 journal article

Physiological function of superoxide dismutase in glucose-limited chemostat cultures of Escherichia coli

Journal of Bacteriology, 130(2), 805–811.

By: H. Hassan* & I. Fridovich

MeSH headings : Escherichia coli / drug effects; Escherichia coli / enzymology; Escherichia coli / growth & development; Free Radicals; Glucose / metabolism; Isoenzymes / analysis; Oxygen / pharmacology; Oxygen Consumption; Streptonigrin / pharmacology; Superoxide Dismutase / analysis; Superoxide Dismutase / physiology; Superoxides / metabolism
TL;DR: Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established, and results indicated a progressive deficiency of reducing power at growth rates below 0.25/h and a surfeit of reducingPower with progressively greater protection against O2- by superoxide dismutase at growth rate greater than 0. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1977 journal article

Regulation of superoxide dismutase synthesis in Escherichia coli: glucose effect

Journal of Bacteriology, 132(2), 505–510.

By: H. Hassan & I. Fridovich

MeSH headings : Cyclic AMP / metabolism; Enzyme Repression; Escherichia coli / enzymology; Escherichia coli / metabolism; Fermentation; Glucose / metabolism; Hydrogen-Ion Concentration; Methylglucosides / pharmacology; Paraquat / pharmacology; Superoxide Dismutase / biosynthesis
TL;DR: It appears likely that superoxide dismutase is controlled directly or indirectly by the intracellular level of O2- and that glucose depressed the level of this enzyme because glucose metabolism is not associated with as rapid a production of O 2- as is the metabolsim of many other substances. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1977 journal article

Regulation of the synthesis of superoxide dismutase in Escherichia coli. Induction by methyl viologen.

Journal of Biological Chemistry, 252(21), 7667–7672.

By: H. Hassan* & I. Fridovich*

TL;DR: It seems likely that paraquat subverts a portion of the normal, cyanide-sensitive, electron flow in the cells and transfers that electron flow to oxygen, in an increased rate of intracellular production of O,-, then induces or derepresses the biosynthesis of the manganese-superoxide dismutase, which defends the cell against the deleterious reactivities of this radical. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1976 journal article

Diminution of outer membrane permeability by Mg2+ in a marine pseudomonad

Journal of Bacteriology, 125(3), 910–915.

By: H. Hassan

MeSH headings : Alkaline Phosphatase / metabolism; Cell Membrane Permeability / drug effects; Cell-Free System; Kinetics; Nitrophenols; Organophosphates / metabolism; Pseudomonas / enzymology; Pseudomonas / metabolism; Seawater; Sodium Chloride / pharmacology; Water Microbiology
TL;DR: Intact cells of the marine pseudomonad MB-45, in the presence of optimal Mg2+, exhibited little alkaline phosphatase activity as judged by the hydrolysis of p-nitrophenylphosphate, but Sonic extracts, in contrast, were rich in this activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1975 chapter

Characterization of methane production from poultry manure

In Managing Livestock Wastes (pp. 244–247, 251). American Society of Agricultural Engineers.

By: H. Hassan, D. Belyea & A. Hassan

Source: NC State University Libraries
Added: March 12, 2022

1975 chapter

Energy recovery and feed production from poultry waste

In W. J. Jewell (Ed.), Energy, Agriculture and Waste Management (pp. 289–305). Michigan, US: Ann Arbor Science Publishers Inc.

By: A. Hassan, H. Hassan & N. Smith

Ed(s): W. Jewell

Source: NC State University Libraries
Added: March 12, 2022

1975 journal article

Kinetics of Na+-dependent K+ ion transport in a marine pseudomonad

Journal of Bacteriology, 121(1), 160–164.

By: H. Hassan* & R. MacLeod

MeSH headings : Biological Transport, Active; Cell Membrane / metabolism; Kinetics; Potassium / metabolism; Potassium Isotopes; Pseudomonadaceae / metabolism; Radioisotopes; Seawater; Sodium / metabolism; Water Microbiology
TL;DR: The results suggest that Na plus and K plus form a ternary complex with a specific K plus carrier molecule on the outer surface of the membrane prior to translocation and the release of K plus inside the cell. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1975 journal article

The resistance of Pseudomonas aeruginosa to chloramphenicol

Canadian Journal of Microbiology, 21(8), 1185–1191.

By: J. Ingram & H. Hassan

MeSH headings : Acetylation; Acridines / pharmacology; Acyltransferases / biosynthesis; Chloramphenicol / metabolism; Chloramphenicol / pharmacology; Drug Resistance, Microbial / drug effects; Genetic Variation; Mitomycins / pharmacology; Pseudomonas aeruginosa / drug effects; Pseudomonas aeruginosa / growth & development; Pseudomonas aeruginosa / metabolism; Pyocyanine / biosynthesis; R Factors / drug effects; Sodium Dodecyl Sulfate / pharmacology
TL;DR: The results suggest that the resistant strain inactivates CM by acetylation and, in addition, develops a "permeability" barrier towards chloramphenicol. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 15, 2021

1973 journal article

Effect of milk solids concentration on buffalo and cow soft cheese

Alexandria Journal of Agricultural Research, 21, 383–389.

By: A. Ismail, N. El Salam & H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

1973 journal article

Some technological aspects of buffalo and cow soft cheese

Alexandria Journal of Agricultural Research, 21, 391–396.

By: A. Ismail, N. El Salam & H. Hassan

Source: NC State University Libraries
Added: March 12, 2022

1969 journal article

Effects of Selected Food Additives on Growth of Pseudomonas fragi

Journal of Dairy Science, 52(3), 335–340.

By: H. Hassan* & E. Collins*

MeSH headings : Bacitracin / pharmacology; Benzoates / pharmacology; Chloramphenicol / pharmacology; Dairy Products; Edetic Acid / pharmacology; Food Additives / pharmacology; Food Microbiology; Food Preservation; Muramidase / pharmacology; Nitrofurazone / pharmacology; Pseudomonas / drug effects; Pseudomonas / growth & development; Tetracycline / pharmacology
TL;DR: Tests of inhibition of Pseudomonas fragi in lactose-yeast extract broth by the food additives chlortctraeycline, nisin, bacitracin, chloramphenicol, lysozyme, ethylenediaminetetraacetic acid (EDTA), nitrofurazone, propyl-p-hydroxybenzoate, sodium benzoates, and potassium sorbate found them ineffective. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1969 journal article

Molar Growth Yield of Streptococcus faecalis on Pyruvate

Journal of Bacteriology, 97(3), 1496–1497.

By: H. Hassan* & E. Collins*

MeSH headings : Culture Media; Enterococcus faecalis / growth & development; Enterococcus faecalis / metabolism; Glucose / metabolism; Pyruvates / metabolism
TL;DR: Y(pyruvate) was 17.3, similar to Y(arginine), for Streptococcus faecalis 6783 grown statically in a complex medium in 1 atm of air. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1969 journal article

Sensory and Shelf-Life Evaluations of Cottage Cheese Treated with Potassium Sorbate

Journal of Dairy Science, 52(4), 439–442.

By: E. Collins* & H. Hassan*

MeSH headings : Antifungal Agents; Dairy Products; Food Preservation; Potassium
TL;DR: These amounts of potassium sorbate retarded growth of bacteria that produced fruity and putrid odors and slime in Cottage cheese at refrigeration temperatures andacteria that produced sourness and molds. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1968 journal article

Molar Growth Yields of Certain Lactic Acid Bacteria as Influenced by Autolysis

Journal of Bacteriology, 96(1), 117–125.

By: H. Hassan* & E. Collins*

MeSH headings : Adenosine Triphosphate / metabolism; Arginine / metabolism; Bacteriological Techniques; Bacteriolysis; Culture Media; Enterococcus faecalis / growth & development; Enterococcus faecalis / metabolism; Glucose / metabolism; Streptococcus / growth & development; Streptococcus / metabolism
TL;DR: Molar growth yields determined from batch cultures of Streptococcus diacetilactis and S. faecalis were appreciably greater at the peaks of maximal growth than after continued incubation and considerable autolysis. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

1968 journal article

Role of Galactose or Glucose-1-Phosphate in Preventing the Lysis of Streptococcus diacetilactis

Journal of Bacteriology, 95(2), 592–602.

By: H. Hassan* & E. Collins*

MeSH headings : Bacterial Proteins / analysis; Bacteriolysis; Chloramphenicol / pharmacology; Galactose / metabolism; Glucose / metabolism; Hexosamines / metabolism; Hexosephosphates / metabolism; Hydrogen-Ion Concentration; Lactose / metabolism; Maltose / metabolism; Osmosis; Streptococcus / metabolism; Temperature
TL;DR: The results indicate that the organism phosphorolyzes maltose to glucose plus beta-glucose-1-phosphate, and suggest that it can use the beta- glucose -1- phosphate in place of alpha-glUCose- 1-ph phosphate in the formation of cell materials. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 13, 2021

Employment

Updated: January 18th, 2023 12:40

1980 - present

North Carolina State University Raleigh, North Carolina, US
Professor of Microbiology, Biochemistry, Food Science, and Toxicology Poultry Science

Education

Updated: January 18th, 2023 12:45

1962 - 1967

University of California Davis Department of Microbiology and Molecular Genetics Davis, CA, US
Ph.D. Microbiology

Funding History

Funding history based on the linked ORCID record. Updated: January 18th, 2023 13:02

grant July 1, 2012 - June 1, 2018
Development of Novel Salmonella control Practices and Integrated Education Program to Reduce Salmonellosis
USDA-NIFA

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