@article{vontimitta_danehower_steede_moon_lewis_2010, title={Analysis of a Nicotiana tabacum L. Genomic Region Controlling Two Leaf Surface Chemistry Traits}, volume={58}, ISSN={["1520-5118"]}, DOI={10.1021/jf903256h}, abstractNote={cis-Abienol and sucrose esters are Nicotiana tabacum leaf surface components that likely influence plant resistance to pests. Their breakdown products also contribute to flavor and aroma characteristics of certain tobacco types. Mapping of genes involved in the biosynthesis of these compounds could permit development of molecular-based tools for generating tobacco types with novel cured leaf chemistry profiles. A doubled haploid mapping population segregating for major genes (Abl and BMVSE) affecting the ability to accumulate cis-abienol and sucrose esters was generated and genotyped with a large set of microsatellite markers. The two genes were found to reside on chromosome A of the N. tabacum genome with a distance of 8.2 cM (centimorgans) between them. Seventeen microsatellite markers were also placed on this linkage group, several of which exhibited complete cosegregation with Abl and BMVSE. Results should aid breeding efforts focused on modification of this aspect of tobacco cured leaf chemistry.}, number={1}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Vontimitta, Vijay and Danehower, David A. and Steede, Tyler and Moon, Hyunsook S. and Lewis, Ramsey S.}, year={2010}, month={Jan}, pages={294–300} }
 @article{lee_shannon_vuong_moon_nguyen_tsukamoto_chung_2010, title={Genetic diversity in wild soybean (Glycine soja Sieb. and Zucc.) accessions from southern islands of Korean peninsula}, volume={129}, ISSN={["0179-9541"]}, DOI={10.1111/j.1439-0523.2009.01757.x}, abstractNote={ With 2 figures and 3 tables }, number={3}, journal={PLANT BREEDING}, author={Lee, J. -D. and Shannon, J. G. and Vuong, T. D. and Moon, H. and Nguyen, H. T. and Tsukamoto, C. and Chung, G.}, year={2010}, month={Jun}, pages={257–263} }
 @article{moon_nicholson_heineman_lion_hoeven_hayes_lewis_2009, title={Changes in Genetic Diversity of US Flue-Cured Tobacco Germplasm over Seven Decades of Cultivar Development}, volume={49}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2008.05.0253}, abstractNote={Plant breeding methodologies have been applied to flue‐cured tobacco (Nicotiana tabacum L.) for approximately seven decades. As has been observed in several other crops, stringent quality requirements have resulted in use of conservative breeding strategies in the development of new cultivars. The impact of breeding practices on genetic diversity within U.S. flue‐cured tobacco germplasm has not been investigated. In this study, we genotyped 117 tobacco cultivars from eight sequential time periods with 71 microsatellite primer pairs. A total of 294 alleles were scored. Only a fraction (48%) of alleles present in the initial germplasm pool was represented in cultivars released during the 1990s and 2000s. Only 13 and 18 alleles were detected in the 1990s and 2000s, respectively, which were undetected in the initial gene pool. The overall trend was one of gradual reduction in allelic counts at microsatellite loci, indicating a reduction in diversity over time at the gene level. Average genetic similarity was highest among cultivars of the 1990s and 2000s, reflecting a reduction in genetic diversity at the population level. This observed narrowing of the U.S. flue‐cured tobacco germplasm base in combination with low rates of genetic gain for yield in the last 20 years may point to a need for diversification of parental materials used in future breeding crosses. Reported genetic relationships among the group of genotyped cultivars may be valuable for future strategic germplasm choices.}, number={2}, journal={CROP SCIENCE}, author={Moon, H. S. and Nicholson, J. S. and Heineman, A. and Lion, K. and Hoeven, P. and Hayes, A. J. and Lewis, R. S.}, year={2009}, pages={498–508} }
 @article{moon_nifong_nicholson_heineman_lion_hoeven_hayes_lewis_2009, title={Microsatellite-based Analysis of Tobacco (Nicotiana tabacum L.) Genetic Resources}, volume={49}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2009.01.0024}, abstractNote={ABSTRACT}, number={6}, journal={CROP SCIENCE}, author={Moon, H. S. and Nifong, J. M. and Nicholson, J. S. and Heineman, A. and Lion, K. and Hoeven, R. and Hayes, A. J. and Lewis, R. S.}, year={2009}, pages={2149–2159} }
 @article{moon_nicholson_lewis_2008, title={Use of transferable Nicotiana tabacum L. microsatellite markers for investigating genetic diversity in the genus Nicotiana}, volume={51}, ISSN={["0831-2796"]}, DOI={10.1139/G08-039}, abstractNote={The recent development of microsatellite markers for tobacco, Nicotiana tabacum L., may be valuable for genetic studies within the genus Nicotiana . The first objective was to evaluate transferability of 100 N. tabacum microsatellite primer combinations to 5 diploid species closely related to tobacco. The number of primer combinations that amplified scorable bands in these species ranged from 42 to 56. Additional objectives were to assess levels of genetic diversity amongst available accessions of diploid relatives closely related to tobacco (species of sections Sylvestres and Tomentosae), and to evaluate the efficacy of microsatellite markers for establishing species relationships in comparison with existing phylogenetic reconstructions. A subset of 46 primer combinations was therefore used to genotype 3 synthetic tobaccos and an expanded collection of 51 Nicotiana accessions representing 15 species. The average genetic similarity for 7 diverse accessions of tobacco was greater than the average similarity for N. otophora accessions, but lower than the average genetic similarities for N. sylvestris , N. tomentosa , N. kawakamii , and N. tomentosiformis accessions. A microsatellite-based phylogenetic tree was largely congruent with taxonomic representations based on morphological, cytological, and molecular observations. Results will be useful for selection of parents for creation of diploid mapping populations and for germplasm introgression activities.}, number={8}, journal={GENOME}, author={Moon, H. S. and Nicholson, J. S. and Lewis, R. S.}, year={2008}, month={Aug}, pages={547–559} }
 @article{moon_nicholson_2007, title={AFLP and SCAR markers linked to Tomato spotted wilt virus resistance in tobacco}, volume={47}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2007.01.0002}, abstractNote={Tomato spotted wilt virus (TSWV) is a serious disease in tobacco (Nicotiana tabacum L.). The breeding line ‘Polalta’ contains a single dominant gene conferring resistance to TSWV that was introgressed from N. alata Link & Otto. The resistance is tightly associated with an abnormal plant type, however, and traditional backcrossing has been ineffective in producing normal plants with TSWV resistance. A potential strategy to overcome this problem is to use molecular markers to select against alien chromatin in backcross progeny. Amplified fragment length polymorphism (AFLP) technology and bulked segregant analysis were applied to identify markers linked to the resistance gene. The DNA bulks from susceptible and resistant doubled haploid lines derived from a cross between susceptible cultivar K326 and Polalta were analyzed to identify linked markers. An F2 population and the doubled haploids were used to construct a 2.5‐cM map of this locus containing 17 coupling phase and seven repulsion phase markers. By selecting for resistant plants with an improved plant type and marker profile in a BC3 population, four plants were identified that were missing 1 to 14 of the AFLP markers associated with the alien chromatin, demonstrating that rare recombination events can be identified. Four AFLP fragments were successfully converted to sequence characterized amplified region markers suitable for large‐scale screening. This approach may facilitate development of TSWV‐resistant tobacco cultivars.}, number={5}, journal={CROP SCIENCE}, author={Moon, H. and Nicholson, J. S.}, year={2007}, pages={1887–1894} }