Hayden Nathaniel Brochu

Works (17)

Updated: February 19th, 2026 16:58

2025 article

Cervicovaginal microbial features predict Chlamydia trachomatis spread to the upper genital tract of infected women

Jeong, S., Tollison, T., Brochu, H., Chou, H., Huntress, I., Yount, K. S., … Peng, X. (2025, August 12). (M. Raffatellu, Ed.). Infection and Immunity, Vol. 8.

By: S. Jeong n, T. Tollison n, H. Brochu n , H. Chou n, I. Huntress n, K. Yount*, X. Zheng *, T. Darville *, C. O'Connell *, X. Peng n 

Ed(s): M. Raffatellu

author keywords: cervicovaginal microbiome; <italic>Chlamydia trachomatis</italic>; upper genital tract infection; 16S rRNA sequencing
topics (OpenAlex): Reproductive tract infections research; Pelvic floor disorders treatments; Urinary Tract Infections Management
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 12, 2025

2025 article

Development of ferret immune repertoire reference resources and single-cell-based high-throughput profiling assays

Walsh, E. S., Yang, K., Tollison, T. S., Seenu, S., Adams, N., Zeitoun, G., … Peng, X. (2025, March 21). Journal of Virology, Vol. 3.

By: E. Walsh n, K. Yang *, T. Tollison n, S. Seenu*, N. Adams n, G. Zeitoun *, I. Sideri *, G. Folch * ...

author keywords: ferret; immune repertoire; reference resource development; VDJ annotation; single-cell sequencing; repertoire profiling
MeSH headings : Animals; Ferrets / immunology; Ferrets / genetics; Single-Cell Analysis / methods; Receptors, Antigen, T-Cell / genetics; Receptors, Antigen, T-Cell / immunology; High-Throughput Nucleotide Sequencing; Transcriptome; Lymph Nodes / immunology; Gene Expression Profiling; Spleen / immunology; Spleen / cytology
topics (OpenAlex): Single-cell and spatial transcriptomics; T-cell and B-cell Immunology; Immune Cell Function and Interaction
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 31, 2025

2025 article

Development of ferret immune repertoire reference resources and single-cell-based high-throughput profiling assays

Walsh, E. S., Yang, K., Tollison, T. S., Seenu, S., Adams, N., Zeitoun, G., … Peng, X. (2025, February 10). (Vol. 2). Vol. 2.

By: E. Walsh n, K. Yang *, T. Tollison n, S. Seenu*, N. Adams n, G. Zeitoun *, I. Sideri *, G. Folch * ...

topics (OpenAlex): Immune Cell Function and Interaction; HIV Research and Treatment; T-cell and B-cell Immunology
Sources: NC State University Libraries, NC State University Libraries
Added: January 18, 2026

2025 article

Diagnostic-avoiding Chlamydia trachomatis variants detected in cervical and endometrial specimens from women during 16S microbiome profiling

Jeong, S., Tollison, T., Brochu, H., Chou, H., Yu, T., Baghaie, P., … O’Connell, C. M. (2025, March 12). BMC Infectious Diseases, Vol. 25.

By: S. Jeong n, T. Tollison n, H. Brochu n , H. Chou n, T. Yu *, P. Baghaie*, K. Yount *, T. Darville * ...

author keywords: NAAT; Chlamydia; Gonorrhea; Microbiome; 16S rRNA; False negative; 23S
MeSH headings : Humans; Female; Chlamydia trachomatis / genetics; Chlamydia trachomatis / isolation & purification; Chlamydia Infections / diagnosis; Chlamydia Infections / microbiology; RNA, Ribosomal, 16S / genetics; Cervix Uteri / microbiology; Microbiota / genetics; Adult; DNA, Bacterial / genetics; Endometrium / microbiology; Gonorrhea / diagnosis; Gonorrhea / microbiology; RNA, Ribosomal, 23S / genetics; Neisseria gonorrhoeae / genetics; Neisseria gonorrhoeae / isolation & purification; Young Adult; Pennsylvania
topics (OpenAlex): Reproductive tract infections research; Syphilis Diagnosis and Treatment; Cervical Cancer and HPV Research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 24, 2025

2024 article

A program for real-time surveillance of SARS-CoV-2 genetics

Brochu, H. N., Song, K., Zhang, Q., Zeng, Q., Shafi, A., Robinson, M., … Iyer, L. K. (2024, April 19). BioRxiv (Cold Spring Harbor Laboratory).

By: H. Brochu* , K. Song *, Q. Zhang *, Q. Zeng *, A. Shafi *, M. Robinson *, J. Humphrey*, B. Croy* ...

topics (OpenAlex): SARS-CoV-2 and COVID-19 Research; CRISPR and Genetic Engineering; SARS-CoV-2 detection and testing
Source: ORCID
Added: April 22, 2024

2024 article

Characterization of vaginal microbiomes in clinician-collected bacterial vaginosis diagnosed samples

Brochu, H. N., Zhang, Q., Song, K., Wang, L., Deare, E. A., Williams, J. D., … Iyer, L. K. (2024, October 1). BioRxiv (Cold Spring Harbor Laboratory).

By: H. Brochu* , Q. Zhang, K. Song, L. Wang, E. Deare, J. Williams, C. Icenhour, L. Iyer

topics (OpenAlex): Reproductive tract infections research; Pelvic floor disorders treatments; Urinary Tract Infections Management
Source: ORCID
Added: October 8, 2024

2024 article

Pre-challenge gut microbial signature predicts RhCMV/SIV vaccine efficacy in rhesus macaques

Brochu, H. N., Smith, E., Jeong, S., Carlson, M., Hansen, S. G., Tisoncik-Go, J., … Peng, X. (2024, February 28).

By: H. Brochu n , E. Smith*, S. Jeong n, M. Carlson *, S. Hansen *, J. Tisoncik-Go *, L. Law *, L. Picker *, M. Gale *, X. Peng n 

topics (OpenAlex): Blood groups and transfusion; HIV Research and Treatment; Hepatitis B Virus Studies; Gut microbiota and health; Clostridium difficile and Clostridium perfringens research
TL;DR: Findings indicate that changes in gut microbiomes are associated with RhCMV/SIV vaccine-induced protection and early host response to vaccination in rhesus macaques. (via Semantic Scholar)
Source: ORCID
Added: March 1, 2024

2024 article

Pre-challenge gut microbial signature predicts RhCMV/SIV vaccine efficacy in rhesus macaques

Brochu, H. N., Smith, E., Jeong, S., Carlson, M., Hansen, S. G., Tisoncik-Go, J., … Peng, X. (2024, September 30). (L. Stamatatos, Ed.). Microbiology Spectrum, Vol. 9.

By: H. Brochu n , E. Smith*, S. Jeong n, M. Carlson *, S. Hansen *, J. Tisoncik-Go *, L. Law *, L. Picker *, M. Gale *, X. Peng n 

Ed(s): L. Stamatatos

author keywords: gut microbiome; immunization; T-cell immunity; rhesus macaque; simian immunodeficiency virus; 16S RNA
MeSH headings : Animals; Macaca mulatta; Gastrointestinal Microbiome / immunology; Simian Immunodeficiency Virus / immunology; Simian Immunodeficiency Virus / genetics; Simian Acquired Immunodeficiency Syndrome / prevention & control; Simian Acquired Immunodeficiency Syndrome / immunology; Simian Acquired Immunodeficiency Syndrome / virology; Simian Acquired Immunodeficiency Syndrome / microbiology; SAIDS Vaccines / immunology; SAIDS Vaccines / administration & dosage; RNA, Ribosomal, 16S / genetics; Cytomegalovirus / immunology; Cytomegalovirus / genetics; Vaccination; Vaccine Efficacy; Bacteria / classification; Bacteria / genetics; Bacteria / immunology; Male
topics (OpenAlex): Gut microbiota and health; Cytomegalovirus and herpesvirus research; HIV Research and Treatment
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: October 1, 2024

2022 article

Alternative splicing and genetic variation of MHC-E: Implications for rhesus cytomegalovirus-based vaccines

Brochu, H., Wang, R., Tollison, T., Pyo, C.-W., Thomas, A., Tseng, E., … Peng, X. (2022, June 14). Research Square (Research Square).

By: H. Brochu n , R. Wang *, T. Tollison  n, C. Pyo*, A. Thomas *, E. Tseng *, L. Law *, L. Picker * ...

topics (OpenAlex): Cytomegalovirus and herpesvirus research; T-cell and B-cell Immunology; Immune Cell Function and Interaction; Viral Infections and Immunology Research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: March 1, 2024

2022 article

Alternative splicing and genetic variation of mhc-e: implications for rhesus cytomegalovirus-based vaccines

Brochu, H., Wang, R., Tollison, T., Pyo, C.-W., Thomas, A., Tseng, E., … Peng, X. (2022, December 19). Communications Biology, Vol. 5.

By: H. Brochu n , R. Wang *, T. Tollison n, C. Pyo*, A. Thomas *, E. Tseng *, L. Law *, L. Picker * ...

MeSH headings : Animals; Humans; Alternative Splicing; Cytomegalovirus; Cytomegalovirus Vaccines; Genetic Variation; Macaca mulatta; Simian Immunodeficiency Virus; Histocompatibility Antigens Class I / genetics
topics (OpenAlex): Cytomegalovirus and herpesvirus research; T-cell and B-cell Immunology; Immune Cell Function and Interaction
TL;DR: Using long-read sequencing, 16 Mamu-E isoforms were identified and all MamU-E splicing junctions were detected among HLA-Eisoforms in humans and the presence of G2_LTR alleles was significantly associated with the lack of RhCMV/SIV vaccine protection. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 17, 2023

2022 article

Phylogeny-guided microbiome OTU-specific association test (POST)

Huang, C., Callahan, B. J., Wu, M. C., Holloway, S. T., Brochu, H., Lu, W., … Tzeng, J.-Y. (2022, June 7). Microbiome, Vol. 6.

By: C. Huang  n, B. Callahan n , M. Wu *, S. Holloway n, H. Brochu n , W. Lu n , X. Peng n , J. Tzeng n 

author keywords: Association test; Phylogenetic tree; Kernel machine regression
MeSH headings : Computational Biology / methods; Computer Simulation; Female; Humans; Infant, Newborn; Microbiota / genetics; Phylogeny; Premature Birth
topics (OpenAlex): Gut microbiota and health; Reproductive tract infections research; Probiotics and Fermented Foods
TL;DR: This work proposes a local collapsing test called phylogeny-guided microbiome OTU-specific association test (POST), and develops a user friendly R package POSTm, which can enhance the selection performance of associated microbiome features by improving the overall true-positive and false-positive detection. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 20, 2022

2022 article

Single-Cell–Based High-Throughput Ig and TCR Repertoire Sequencing Analysis in Rhesus Macaques

Walsh, E. S., Tollison, T. S., Brochu, H. N., Shaw, B. I., Diveley, K. R., Chou, H., … Peng, X. (2022, January 5). The Journal of Immunology, Vol. 208, pp. 762–771.

By: E. Walsh n, T. Tollison n, H. Brochu n , B. Shaw *, K. Diveley n, H. Chou n, L. Law *, A. Kirk *, M. Gale *, X. Peng n 

MeSH headings : Animals; Gene Expression Profiling; High-Throughput Nucleotide Sequencing; Immunoglobulins / genetics; Macaca mulatta; Receptors, Antigen, T-Cell, alpha-beta / genetics; Receptors, Antigen, T-Cell, gamma-delta / genetics; Single-Cell Analysis; T-Lymphocytes / immunology; Transcriptome / genetics; VDJ Exons / genetics
topics (OpenAlex): T-cell and B-cell Immunology; Single-cell and spatial transcriptomics; Immune Cell Function and Interaction
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 28, 2022

2021 article

Tumor Necrosis Factor Alpha-Induced Interleukin-1 Alpha Synthesis and Cell Death Is Increased in Mouse Epithelial Cells Infected With Chlamydia muridarum

Nagarajan, U. M., Cho, C., Gyorke, C. E., Nagarajan, S., Ezzell, J. A., Brochu, H., … Peng, X. (2021, March 25). The Journal of Infectious Diseases, Vol. 224, pp. S47–S55.

By: U. Nagarajan *, C. Cho *, C. Gyorke*, S. Nagarajan*, J. Ezzell *, H. Brochu n , I. Huntress n, E. Harrell n, X. Peng n 

author keywords: Chlamydia muridarum; IL-1 alpha; mouse epithelial cells; mouse model; TNF alpha
MeSH headings : Animals; Cell Death; Chlamydia Infections / immunology; Chlamydia Infections / metabolism; Chlamydia muridarum / immunology; Epithelial Cells; Female; Interleukin-1alpha / immunology; Interleukin-1alpha / metabolism; Mice; Mice, Inbred C57BL; Tumor Necrosis Factor-alpha / immunology; Tumor Necrosis Factor-alpha / metabolism
topics (OpenAlex): Reproductive tract infections research; Immune Response and Inflammation; Reproductive Physiology in Livestock
TL;DR: The results suggest TNFα and IL-1α enhance each other's downstream effects resulting in a hyperinflammatory response to chlamydial infection and propose that biologics targeting TNF-induced IL- 1α synthesis could be used to mitigate tissue damage during chlamYDial infection. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 13, 2021

2020 article

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis

Dinh, P.-U. C., Paudel, D., Brochu, H., Popowski, K. D., Gracieux, M. C., Cores, J., … Cheng, K. (2020, February 28). Nature Communications, Vol. 11.

By: P. Dinh n, D. Paudel n, H. Brochu n , K. Popowski n , M. Gracieux n, J. Cores n, K. Huang n , M. Hensley n ...

MeSH headings : Administration, Inhalation; Alveolar Epithelial Cells / drug effects; Alveolar Epithelial Cells / metabolism; Alveolar Epithelial Cells / pathology; Animals; Apoptosis / drug effects; Bleomycin / toxicity; Cell Proliferation; Disease Models, Animal; Exosomes / metabolism; Exosomes / transplantation; Humans; Idiopathic Pulmonary Fibrosis / chemically induced; Idiopathic Pulmonary Fibrosis / metabolism; Idiopathic Pulmonary Fibrosis / pathology; Idiopathic Pulmonary Fibrosis / therapy; Lung / cytology; Lung / metabolism; Lung Injury / chemically induced; Lung Injury / metabolism; Lung Injury / pathology; Lung Injury / therapy; Mesenchymal Stem Cells / metabolism; Mice; Myofibroblasts / cytology; Proteomics; Silicon Dioxide / toxicity; Spheroids, Cellular / metabolism
topics (OpenAlex): Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis; Medical Imaging and Pathology Studies; Extracellular vesicles in disease
TL;DR: It is shown that the secretome and exosomes of lung spheroid cells is effective as inhalation treatment in rodent models of lung injury and fibrosis and superior to the counterparts derived from mesenchymal stem cells. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 23, 2020

2020 article

Systematic Profiling of Full-Length Ig and TCR Repertoire Diversity in Rhesus Macaque through Long Read Transcriptome Sequencing

Brochu, H. N., Tseng, E., Smith, E., Thomas, M. J., Jones, A. M., Diveley, K. R., … Peng, X. (2020, May 6). The Journal of Immunology, Vol. 204, pp. 3434–3444.

By: H. Brochu n , E. Tseng *, E. Smith*, M. Thomas *, A. Jones n, K. Diveley n, L. Law *, S. Hansen * ...

MeSH headings : Animals; High-Throughput Nucleotide Sequencing / methods; Humans; Immunoglobulins / genetics; Immunoglobulins / immunology; Macaca mulatta / genetics; Macaca mulatta / immunology; Receptors, Antigen, T-Cell / immunology; Transcriptome / genetics; Transcriptome / immunology
topics (OpenAlex): Single-cell and spatial transcriptomics; Immune Cell Function and Interaction; T-cell and B-cell Immunology
TL;DR: New rhesus-specific assays are designed that remove the need for primers conventionally targeting variable regions and allow single cell level Ig and TCR repertoire analysis, and the first complete reference set for the constant regions of all known isotypes and chain types of rhesu Ig andTCR repertoires is constructed. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: July 6, 2020

2019 article

Simultaneous profiling of sexually transmitted bacterial pathogens, microbiome, and concordant host response in cervical samples using whole transcriptome sequencing analysis

O'Connell, C. M., Brochu, H., Girardi, J., Harrell, E., Jones, A., Darville, T., … Peng, X. (2019, February 20). Microbial Cell, Vol. 6, pp. 177–183.

By: C. O'Connell *, H. Brochu n , J. Girardi *, E. Harrell n, A. Jones n, T. Darville *, A. Seña *, X. Peng n 

author keywords: RNA-seq; chlamydia; gonorrhea; natural infection; microbiome
topics (OpenAlex): Reproductive tract infections research; Reproductive System and Pregnancy; Pelvic floor disorders treatments
TL;DR: It is suggested that total RNA profiling will advance understanding of the interplay of pathogen, host and microbiota during natural infection and may reveal novel, outcome-relevant biomarkers. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 18, 2019

2017 review

Elucidating the role of host long non-coding RNA during viral infection: Challenges and paths forward

[Review of ]. Vaccines, 5(4).

By: D. Lemler, H. Brochu , F. Yang, E. Harrell & X. Peng 

Source: NC State University Libraries
Added: August 6, 2018

Employment

Updated: April 17th, 2024 07:50

2021 - present

LabCorp (United States) Portland, Oregon, US
Senior Bioinformatics Scientist

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