Cloning, Over-Expression, and Purification of Carbonic Anhydrase from an Extremophilic Bacterium: An Introduction to Advanced Molecular Biology
Somalinga, V., Klemmer, H., Arun, A., Mathews, S., Wapshott, H., & Grunden, A. M. (2017, December 21). The American Biology Teacher, Vol. 80, pp. 29–34.
author keywords: research methods; high school student research program; carbonic anhydrase; protein purification; protonography; Photobacterium profundum
topics (OpenAlex): Enzyme function and inhibition; Enzyme Catalysis and Immobilization; Polyamine Metabolism and Applications
TL;DR:
Successful cloning and over-expression by high school students of two novel carbonic anhydrases from a psychrohalophile with application in biofuel production are reported.
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UN Sustainable Development Goal Categories
14. Life Below Water
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Sources: Web Of Science, NC State University Libraries, ORCID