@article{chamberlin_bennett_isleib_copeland_dunne_2022, title={Registration of 'Comrade' peanut}, volume={8}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20233}, abstractNote={Abstract}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Chamberlin, Kelly and Bennett, Rebecca S. and Isleib, Thomas George and Copeland, Susan and Dunne, Jeffrey C.}, year={2022}, month={Aug} }
 @article{chu_clevenger_holbrook_isleib_ozias-akins_2022, title={Registration of two peanut recombinant inbred lines (TifGP-5 and TifGP-6) resistant to late leaf spot disease}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20242}, abstractNote={Abstract}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Chu, Ye and Clevenger, Josh P. and Holbrook, C. Corley and Isleib, Thomas G. and Ozias-Akins, Peggy}, year={2022}, month={Jul} }
 @article{wang_chen_dang_carter_zhao_lamb_chu_holbrook_ozias-akins_isleib_et al._2022, title={Variabilities in symbiotic nitrogen fixation and carbon isotope discrimination among peanut (Arachis hypogaea L.) genotypes under drought stress}, ISSN={["1439-037X"]}, DOI={10.1111/jac.12619}, abstractNote={Abstract}, journal={JOURNAL OF AGRONOMY AND CROP SCIENCE}, author={Wang, Xu and Chen, Charles Y. and Dang, Phat and Carter, Joshua and Zhao, Shuli and Lamb, Marshall C. and Chu, Ye and Holbrook, Corley and Ozias-Akins, Peggy and Isleib, Thomas G. and et al.}, year={2022}, month={Jul} }
 @article{luo_cui_chavarro_tseng_zhou_peng_chi_yang_lopez_tillman_et al._2020, title={Mapping quantitative trait loci (QTLs) and estimating the epistasis controlling stem rot resistance in cultivated peanut (Arachis hypogaea)}, volume={133}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-020-03542-y}, abstractNote={A total of 33 additive stem rot QTLs were identified in peanut genome with nine of them consistently detected in multiple years or locations. And 12 pairs of epistatic QTLs were firstly reported for peanut stem rot disease. Stem rot in peanut (Arachis hypogaea) is caused by the Sclerotium rolfsii and can result in great economic loss during production. In this study, a recombinant inbred line population from the cross between NC 3033 (stem rot resistant) and Tifrunner (stem rot susceptible) that consists of 156 lines was genotyped by using 58 K peanut single nucleotide polymorphism (SNP) array and phenotyped for stem rot resistance at multiple locations and in multiple years. A linkage map consisting of 1451 SNPs and 73 simple sequence repeat (SSR) markers was constructed. A total of 33 additive quantitative trait loci (QTLs) for stem rot resistance were detected, and six of them with phenotypic variance explained of over 10% (qSR.A01-2, qSR.A01-5, qSR.A05/B05-1, qSR.A05/B05-2, qSR.A07/B07-1 and qSR.B05-1) can be consistently detected in multiple years or locations. Besides, 12 pairs of QTLs with epistatic (additive × additive) interaction were identified. An additive QTL qSR.A01-2 also with an epistatic effect interacted with a novel locus qSR.B07_1-1 to affect the percentage of asymptomatic plants in a row. A total of 193 candidate genes within 38 stem rot QTLs intervals were annotated with functions of biotic stress resistance such as chitinase, ethylene-responsive transcription factors and pathogenesis-related proteins. The identified stem rot resistance QTLs, candidate genes, along with the associated SNP markers in this study, will benefit peanut molecular breeding programs for improving stem rot resistance.}, number={4}, journal={THEORETICAL AND APPLIED GENETICS}, author={Luo, Ziliang and Cui, Renjie and Chavarro, Carolina and Tseng, Yu-Chien and Zhou, Hai and Peng, Ze and Chi, Ye and Yang, Xiping and Lopez, Yolanda and Tillman, Barry and et al.}, year={2020}, month={Apr}, pages={1201–1212} }
 @article{gangurde_wang_yaduru_pandey_fountain_chu_isleib_holbrook_xavier_culbreath_et al._2020, title={Nested-association mapping (NAM)-based genetic dissection uncovers candidate genes for seed and pod weights in peanut (Arachis hypogaea)}, volume={18}, ISSN={["1467-7652"]}, DOI={10.1111/pbi.13311}, abstractNote={Summary}, number={6}, journal={PLANT BIOTECHNOLOGY JOURNAL}, author={Gangurde, Sunil S. and Wang, Hui and Yaduru, Shasidhar and Pandey, Manish K. and Fountain, Jake C. and Chu, Ye and Isleib, Thomas and Holbrook, C. Corley and Xavier, Alencar and Culbreath, Albert K. and et al.}, year={2020}, month={Jun}, pages={1457–1471} }
 @article{chavarro_chu_holbrook_isleib_bertioli_hovav_butts_lamb_sorensen_jackson_et al._2020, title={Pod and Seed Trait QTL Identification To Assist Breeding for Peanut Market Preferences}, volume={10}, ISSN={["2160-1836"]}, DOI={10.1534/g3.120.401147}, abstractNote={Abstract}, number={7}, journal={G3-GENES GENOMES GENETICS}, author={Chavarro, Carolina and Chu, Ye and Holbrook, Corley and Isleib, Thomas and Bertioli, David and Hovav, Ran and Butts, Christopher and Lamb, Marshall and Sorensen, Ronald and Jackson, Scott A. and et al.}, year={2020}, month={Jul}, pages={2297–2315} }
 @article{cui_clevenger_chu_brenneman_isleib_holbrook_ozias-akins_2020, title={Quantitative trait loci sequencing-derived molecular markers for selection of stem rot resistance in peanut}, volume={60}, ISSN={["1435-0653"]}, DOI={10.1002/csc2.20047}, abstractNote={Abstract}, number={4}, journal={CROP SCIENCE}, author={Cui, Renjie and Clevenger, Josh and Chu, Ye and Brenneman, Timothy and Isleib, Thomas G. and Holbrook, C. Corley and Ozias-Akins, Peggy}, year={2020}, pages={2008–2018} }
 @article{balota_isleib_2020, title={Registration of GP-VT NC 01 peanut germplasm}, volume={14}, ISSN={["1940-3496"]}, DOI={10.1002/plr2.20028}, abstractNote={Abstract}, number={2}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Balota, M. and Isleib, T. G.}, year={2020}, month={May}, pages={172–178} }
 @article{hancock_tallury_isleib_chu_ozias-akins_stalker_2019, title={Introgression Analysis and Morphological Characterization of an Arachis hypogaea x A. diogoi Interspecific Hybrid Derived Population}, volume={59}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2018.07.0461}, abstractNote={Cultivated peanut (Arachis hypogaea L.) is an economically important crop grown around the world. Compared with the entire Arachis genus, cultivated peanut germplasm has low levels of genetic diversity for several economically important traits, resulting in the need for alternative sources of favorable alleles. Wild diploid species of Arachis are a source of such alleles to improve cultivated peanut for many economically important traits. An A. hypogaea × A. diogoi Hoehne introgression population was produced via the triploid–hexaploid method; the fourth generation after tetraploidy was used to initiate this study. The introgression lines were genotyped using a single nucleotide polymorphism (SNP) marker array to estimate the percentage of A. diogoi chromatin introgression. Morphologically, the introgression lines varied for an array of measured traits, with the majority being intermediate to the two parents. The average amount of A. diogoi genome introgressed was 8.12% across the tetraploid genome and ranged from 3.00 to 18.14% on individual chromosomes. The average A. diogoi introgression across all lines was 7.70% and ranged from 0.17 to 51.12%. Principal component analysis of morphological data and SNP markers revealed similarities and groupings of introgression lines. This introgression population demonstrates the potential of using wild diploid Arachis species for peanut improvement and has great potential for use in cultivated peanut breeding programs.}, number={2}, journal={CROP SCIENCE}, author={Hancock, Wesley G. and Tallury, Shyam P. and Isleib, Thomas G. and Chu, Ye and Ozias-Akins, Peggy and Stalker, H. Thomas}, year={2019}, pages={640–649} }
 @article{chu_chee_culbreath_isleib_holbrook_ozias-akins_2019, title={Major QTLs for Resistance to Early and Late Leaf Spot Diseases Are Identified on Chromosomes 3 and 5 in Peanut (Arachis hypogaea)}, volume={10}, ISSN={["1664-462X"]}, DOI={10.3389/fpls.2019.00883}, abstractNote={Early and late leaf spots are the major foliar diseases of peanut responsible for severely decreased yield in the absence of intensive fungicide spray programs. Pyramiding host resistance to leaf spots in elite cultivars is a sustainable solution to mitigate the diseases. In order to determine the genetic control of leaf spot disease resistance in peanut, a recombinant inbred line population (Florida-07 x GP-NC WS16) segregating for resistance to both diseases was used to construct a SNP-based linkage map consisting of 855 loci. QTL mapping revealed three resistance QTLs for late leaf spot qLLSA05 (phenotypic variation explained, PVE=7-10%), qLLSB03 (PVE=5-7%), and qLLSB05 (PVE=15-41%) that were consistently expressed over multi-year analysis. Two QTL, qLLSA05 and qLLSB05, confirmed our previously published QTL-seq results. For early leaf spot, three resistance QTLs were identified in multiple years, two on chromosome A03 (PVE=8-12%) and one on chromosome B03 (PVE=13-20%), with the locus qELSA03_1.1 coinciding with the previously published genomic region for LLS resistance in GPBD4. Comparative analysis of the genomic regions spanning the QTLs suggests that resistance to early and late leaf spots are largely genetically independent. In addition, QTL analysis on yield showed that the presence of resistance allele in qLLSB03 and qLLSB05 loci might result in protection from yield loss caused by LLS disease damage. Finally, post hoc analysis of the RIL subpopulation that was not utilized in the QTL mapping revealed that the flanking markers for these QTLs can successfully select for resistant and susceptible lines, confirming the effectiveness of pyramiding these resistance loci to improve host-plant resistance in peanut breeding programs using marker-assisted selection.}, journal={FRONTIERS IN PLANT SCIENCE}, author={Chu, Ye and Chee, Peng and Culbreath, Albert and Isleib, Thomas G. and Holbrook, C. Corley and Ozias-Akins, Peggy}, year={2019}, month={Jul} }
 @article{chu_chee_isleib_holbrook_ozias-akins_2019, title={Major seed size QTL on chromosome A05 of peanut (Arachis hypogaea) is conserved in the US mini core germplasm collection}, volume={40}, ISSN={["1572-9788"]}, DOI={10.1007/s11032-019-1082-4}, abstractNote={Abstract}, number={1}, journal={MOLECULAR BREEDING}, author={Chu, Ye and Chee, Peng and Isleib, Thomas G. and Holbrook, C. Corley and Ozias-Akins, Peggy}, year={2019}, month={Dec} }
 @article{sinclair_shekoofa_isleib_balota_zhang_2018, title={Identification of Virginia-Type Peanut Genotypes for Water-Deficit Conditions Based on Early Decrease in Transpiration Rate with Soil Drying}, volume={58}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2018.05.0293}, abstractNote={Early decrease in transpiration rate as soil progressively dries allows soil water conservation for sustained crop physiological activity as water deficit continues to increase. This trait is likely to be particularly useful for peanut (Arachis hypogaea L.), which is often grown on sandy soil where water deficit can develop quickly. This study was undertaken to identify peanut genotypes that express this water conservation trait and to determine if it confers a yield advantage. Three approaches were taken. (i) Two populations of peanut were tested in controlled environments during progressive soil drying. Especially high thresholds of soil water content for initiation of decrease in transpiration rate were identified in three lines in each of the breeding populations. (ii) Nine genotypes were identified for field observation of leaf wilting under rain shelters and in the open field. There was a correspondence between the early decrease in transpiration rate with soil drying and delayed wilting. (iii) Yield trial data for three genotypes were examined to identify those lines that consistently had higher yields than the commercial check cultivar ‘Bailey’, particularly at low yield levels commonly associated with drier conditions. When expressing total seed yield in monetary return, these breeding lines had yield values that were consistently superior to Bailey below the US$2000 ha−1 threshold. Based on the three experimental approaches, N12006ol consistently expressed a desired response of early decrease in transpiration rate with soil drying, delayed wilting in the field when soil water deficit developed, and greater yield value than Bailey in low‐yield environments.}, number={6}, journal={CROP SCIENCE}, author={Sinclair, Thomas R. and Shekoofa, Avat and Isleib, Thomas G. and Balota, Maria and Zhang, Hao}, year={2018}, pages={2607–2612} }
 @article{clevenger_chu_chavarro_botton_culbreath_isleib_holbrook_ozias-akins_2018, title={Mapping Late Leaf Spot Resistance in Peanut (Arachis hypogaea) Using QTL-seq Reveals Markers for Marker-Assisted Selection}, volume={9}, ISSN={["1664-462X"]}, DOI={10.3389/fpls.2018.00083}, abstractNote={Late leaf spot (LLS; Cercosporidium personatum) is a major fungal disease of cultivated peanut (Arachis hypogaea). A recombinant inbred line population segregating for quantitative field resistance was used to identify quantitative trait loci (QTL) using QTL-seq. High rates of false positive SNP calls using established methods in this allotetraploid crop obscured significant QTLs. To resolve this problem, robust parental SNPs were first identified using polyploid-specific SNP identification pipelines, leading to discovery of significant QTLs for LLS resistance. These QTLs were confirmed over 4 years of field data. Selection with markers linked to these QTLs resulted in a significant increase in resistance, showing that these markers can be immediately applied in breeding programs. This study demonstrates that QTL-seq can be used to rapidly identify QTLs controlling highly quantitative traits in polyploid crops with complex genomes. Markers identified can then be deployed in breeding programs, increasing the efficiency of selection using molecular tools. Key Message: Field resistance to late leaf spot is a quantitative trait controlled by many QTLs. Using polyploid-specific methods, QTL-seq is faster and more cost effective than QTL mapping.}, journal={FRONTIERS IN PLANT SCIENCE}, author={Clevenger, Josh and Chu, Ye and Chavarro, Carolina and Botton, Stephanie and Culbreath, Albert and Isleib, Thomas G. and Holbrook, C. C. and Ozias-Akins, Peggy}, year={2018}, month={Feb} }
 @article{shekoofa_sinclair_aninbon_holbrook_isleib_ozias-akins_chu_2017, title={Expression of the limited-transpiration trait under high vapour pressure deficit in peanut populations: Runner and virginia types}, volume={203}, ISSN={["1439-037X"]}, DOI={10.1111/jac.12204}, abstractNote={Abstract}, number={4}, journal={JOURNAL OF AGRONOMY AND CROP SCIENCE}, author={Shekoofa, A. and Sinclair, T. R. and Aninbon, C. and Holbrook, C. C. and Isleib, T. G. and Ozias-Akins, P. and Chu, Y.}, year={2017}, month={Aug}, pages={295–300} }
 @article{clevenger_chu_chavarro_agarwal_bertioli_leal-bertioli_pandey_vaughn_abernathy_barkley_et al._2017, title={Genome-wide SNP Genotyping Resolves Signatures of Selection and Tetrasomic Recombination in Peanut}, volume={10}, ISSN={["1752-9867"]}, DOI={10.1016/j.molp.2016.11.015}, abstractNote={Peanut (Arachis hypogaea; 2n = 4x = 40) is a nutritious food and a good source of vitamins, minerals, and healthy fats. Expansion of genetic and genomic resources for genetic enhancement of cultivated peanut has gained momentum from the sequenced genomes of the diploid ancestors of cultivated peanut. To facilitate high-throughput genotyping of Arachis species, 20 genotypes were re-sequenced and genome-wide single nucleotide polymorphisms (SNPs) were selected to develop a large-scale SNP genotyping array. For flexibility in genotyping applications, SNPs polymorphic between tetraploid and diploid species were included for use in cultivated and interspecific populations. A set of 384 accessions was used to test the array resulting in 54 564 markers that produced high-quality polymorphic clusters between diploid species, 47 116 polymorphic markers between cultivated and interspecific hybrids, and 15 897 polymorphic markers within A. hypogaea germplasm. An additional 1193 markers were identified that illuminated genomic regions exhibiting tetrasomic recombination. Furthermore, a set of elite cultivars that make up the pedigree of US runner germplasm were genotyped and used to identify genomic regions that have undergone positive selection. These observations provide key insights on the inclusion of new genetic diversity in cultivated peanut and will inform the development of high-resolution mapping populations. Due to its efficiency, scope, and flexibility, the newly developed SNP array will be very useful for further genetic and breeding applications in Arachis.}, number={2}, journal={MOLECULAR PLANT}, author={Clevenger, Josh and Chu, Ye and Chavarro, Carolina and Agarwal, Gaurav and Bertioli, David J. and Leal-Bertioli, Soraya C. M. and Pandey, Manish K. and Vaughn, Justin and Abernathy, Brian and Barkley, Noelle A. and et al.}, year={2017}, month={Feb}, pages={309–322} }
 @article{koppelman_jayasena_luykx_schepens_apostolovic_jong_isleib_nordlee_baumert_taylor_et al._2016, title={Allergenicity attributes of different peanut market types}, volume={91}, ISSN={["1873-6351"]}, DOI={10.1016/j.fct.2016.02.016}, abstractNote={Four different market classes of peanut (Runner, Virginia Spanish, and Valencia) are commonly consumed in Western countries, but for some consumers peanuts are a main cause of food-induced anaphylaxis. Limited information is available on the comparative allergenicity of these distinct market classes. The aim of this study was to compare allergenicity attributes of different peanut cultivars. The protein content and protein profiles were highly comparable for all tested cultivars. All cultivar samples contained the major allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6, as assessed by SDS-PAGE and RP-HPLC, although some minor differences in major allergen content were found between samples. All samples were reactive in commercial ELISAs for detection and quantification of peanut protein. IgE-binding potency differed between samples with a maximum factor of 2, indicating a highly comparable allergenicity. Based on our observations, we conclude that peanuts from the main market types consumed in Western countries are highly comparable in their allergenicity attributes, indicating that safety considerations with regard to peanut allergy are not dependent on the peanut cultivar in question.}, journal={FOOD AND CHEMICAL TOXICOLOGY}, author={Koppelman, Stef J. and Jayasena, Shyamali and Luykx, Dion and Schepens, Erik and Apostolovic, Danijela and Jong, Govardus A. H. and Isleib, Thomas G. and Nordlee, Julie and Baumert, Joe and Taylor, Steve L. and et al.}, year={2016}, month={May}, pages={82–90} }
 @article{kimball_isleib_reynolds_zuleta_milla-lewis_2016, title={Combining ability for winter survival and turf quality traits in st. augustinegrass}, volume={51}, number={7}, journal={HortScience}, author={Kimball, J. A. and Isleib, T. G. and Reynolds, W. C. and Zuleta, M. C. and Milla-Lewis, S. R.}, year={2016}, pages={810–815} }
 @article{livingston_tuong_isleib_murphy_2016, title={Differences between wheat genotypes in damage from freezing temperatures during reproductive growth}, volume={74}, ISSN={["1873-7331"]}, DOI={10.1016/j.eja.2015.12.002}, abstractNote={Cereal crops in the reproductive stage of growth are considerably more susceptible to injury from freezing temperatures than during their vegetative growth stage in the fall. While damage resulting from spring-freeze events has been documented, information on genotypic differences in tolerance to spring-freezes is scarce. Ninety wheat genotypes were subjected to a simulated spring-freeze at the mid-boot growth stage under controlled conditions. Spring-freeze tolerance was evaluated as the number of seeds per head at maturity after plants were frozen at −6 °C. Plants that froze, as confirmed by infrared (IR) thermography, died shortly after thawing and consequently the heads did not mature. Only in plants that had no visible freezing (super-cooled) were heads able to reach maturity and produce seeds. In plants that super-cooled four genotypes had significantly higher seed counts after being exposed to freezing than three with the lowest. In addition, significant differences between genotypes were found in whole plant survival among those that had frozen. Genotypes with high whole-plant freezing survival were not necessarily the same as the super-cooled plants with the highest seed counts. Spring-freeze tolerance was not correlated with maturity suggesting that improvement in freezing tolerance could be selected for without affecting heading date. Spring-freeze tolerance was not correlated with freezing tolerance of genotypes of plants in a vegetative state, either under non-acclimated or cold-acclimated conditions indicating that vegetative freezing tolerance is not a good predictor of spring-freeze tolerance.}, journal={EUROPEAN JOURNAL OF AGRONOMY}, author={Livingston, David P., III and Tuong, Tan D. and Isleib, Thomas G. and Murphy, J. Paul}, year={2016}, month={Mar}, pages={164–172} }
 @article{shekoofa_rosas-anderson_sinclair_balota_isleib_2015, title={Measurement of Limited-Transpiration Trait under High Vapor Pressure Deficit for Peanut in Chambers and in Field}, volume={107}, ISSN={["1435-0645"]}, DOI={10.2134/agronj14.0570}, abstractNote={Drought is one of the most important environmental factors that limit crop production. Based on controlled‐environment studies, it has been hypothesized that a limited‐transpiration (TRlim) trait under high vapor pressure deficit (VPD) is a mechanism for water conservation leading to yield increase under water‐deficit conditions. The current research objective was to compare expression of TRlim in peanut (Arachis hypogaea L.) observed by whole‐plant measurements in controlled environments and by leaf gas exchange measurements on plants grown in the field. Six peanut genotypes with different breeding backgrounds, that is, wild‐type, commercial cultivars, and advanced breeding lines were studied. Differences were observed among genotypes in their expression of TRlim with increasing VPD in the controlled environment at 31/26°C. Within each breeding background, one genotype showed a linear increase in transpiration with increasing VPD while the other expressed the TRlim trait. In a second set of controlled environment experiments at 36/26°C, none of the six genotypes expressed the TRlim trait. In the field, again none of the genotypes expressed the TRlim trait. The temperature to which the plants were exposed between the two controlled environments and field trial appeared critical in the expression of the TRlim trait of three of the genotypes.}, number={3}, journal={AGRONOMY JOURNAL}, author={Shekoofa, Avat and Rosas-Anderson, Pablo and Sinclair, Thomas R. and Balota, Maria and Isleib, Thomas G.}, year={2015}, pages={1019–1024} }
 @article{isleib_milla-lewis_pattee_copeland_zuleta_shew_hollowell_sanders_dean_hendrix_et al._2015, title={Registration of ‘Sugg’ peanut}, volume={9}, ISSN={["1940-3496"]}, DOI={10.3198/jpr2013.09.0059crc}, abstractNote={‘Sugg’ (Reg. No. CV-125, PI 666112) is a large-seeded virginia-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) cultivar with partial resistance to four diseases that occur commonly in the Virginia–Carolina production area: early leafspot caused by Cercospora arachidicola S. Hori, Cylindrocladium black rot caused by Cylindrocladium parasiticum Crous, Wingfield & Alfenas, Sclerotinia blight caused by Sclerotinia minor Jagger, and tomato spotted wilt caused by the Tomato spotted wilt tospovirus. Sugg was developed as part of a program of selection for multiple disease resistance funded by growers, seed dealers, shellers, and processors. Sugg was tested under the experimental designation N03091T and released by the North Carolina Agricultural Research Service (NCARS) in 2009. Sugg was tested by the NCARS, the Virginia Agricultural Experiment Station, and five other state agricultural experiment stations and the USDA–ARS units participating in the Uniform Peanut Performance Tests. Sugg has alternate branching pattern, intermediate runner growth habit, medium green foliage, and high contents of fancy pods and medium virginia-type seeds. It has seeds with pink testa averaging 957 mg seed−1, approximately 40% jumbo and 46% fancy pods, and extra-large kernel content of ∼47%. Sugg is named in honor of Norfleet “Fleet” Sugg and the late Joseph “Joe” Sugg, cousins who served consecutively as executive directors of the North Carolina Peanut Growers Association from 1966 through 1993.}, number={1}, journal={J. Plant Reg.}, publisher={American Society of Agronomy}, author={Isleib, T.G. and Milla-Lewis, S.R. and Pattee, H.E. and Copeland, S.C. and Zuleta, M.C. and Shew, B.B. and Hollowell, J.E. and Sanders, T.H. and Dean, L.O. and Hendrix, K.W. and et al.}, year={2015}, pages={44–52} }
 @article{singh_collakova_isleib_welbaum_tallury_balota_2014, title={Differential Physiological and Metabolic Responses to Drought Stress of Peanut Cultivars and Breeding Lines}, volume={54}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.09.0606}, abstractNote={ABSTRACT}, number={5}, journal={CROP SCIENCE}, author={Singh, Daljit and Collakova, Eva and Isleib, Thomas G. and Welbaum, Gregory E. and Tallury, Shyam P. and Balota, Maria}, year={2014}, pages={2262–2274} }
 @article{rosas-anderson_sinclair_balota_tallury_isleib_rufty_2014, title={Genetic Variation for Epidermal Conductance in Peanut}, volume={54}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.07.0461}, abstractNote={ABSTRACT}, number={2}, journal={CROP SCIENCE}, author={Rosas-Anderson, Pablo and Sinclair, Thomas R. and Balota, Maria and Tallury, Shyam and Isleib, Thomas G. and Rufty, Thomas}, year={2014}, pages={730–737} }
 @article{rosas-anderson_shekoofa_sinclair_balota_isleib_tallury_rufty_2014, title={Genetic variation in peanut leaf maintenance and transpiration recovery from severe soil drying}, volume={158}, ISSN={0378-4290}, url={http://dx.doi.org/10.1016/J.FCR.2013.12.019}, DOI={10.1016/J.FCR.2013.12.019}, abstractNote={Peanut (Arachis hypogaea L.) is an important food crop that is often grown in areas prone to intermittent drought. After drought is relieved, plant recovery from soil drying is an important factor for continued productivity. While recovery can involve a multitude of physiological processes, transpiration is one of the most important for carbon fixation. Two greenhouse experiments and a field experiment were conducted to screen and evaluate a total of 19 peanut genotypes for transpiration recovery and leaf maintenance after experiencing a drying cycle. In the greenhouse experiments, plants were allowed to transpire all available transpirable soil water from their pots before being re-watered. The transpiration of plants was measured in subsequent days and a visual rating scale was used to rate leaf maintenance on plants. Significant differences were detected among genotypes for both transpiration recovery and leaf maintenance, and superior genotypes were identified for both traits. The superior genotypes included ICGV 86015, TMV 2, PI 497579 and PI 404020 in the greenhouse. In the field, a regression between stomatal conductance before and after re-watering gave an estimate for recovery of stomatal conductance. Breeding lines N05006 and SPT 06-07 had the greatest estimated stomata conductance recovery in the field. Genotypes which recovered poorly in the greenhouse also had low estimated recovery in the field.}, journal={Field Crops Research}, publisher={Elsevier BV}, author={Rosas-Anderson, Pablo and Shekoofa, Avat and Sinclair, Thomas R. and Balota, Maria and Isleib, Thomas G. and Tallury, Shyam and Rufty, Thomas}, year={2014}, month={Mar}, pages={65–72} }
 @article{tallury_isleib_copeland_rosas-anderson_balota_singh_stalker_2014, title={Registration of Two Multiple Disease-Resistant Peanut Germplasm Lines Derived from Arachis cardenasii Krapov. & WC Gregory, GKP 10017}, volume={8}, ISSN={["1940-3496"]}, DOI={10.3198/jpr2013.04.0017crg}, abstractNote={Two tetraploid (2n = 4x = 40) peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) germplasm lines, GP-NC WS 16 (SPT 06-06) (Reg. No. GP-235, PI 669445) and GP-NC WS 17 (SPT 06-07) (Reg. No. GP-236, PI 669446), derived from interspecific hybridization, were developed in the peanut genetics program at North Carolina State University (NCSU), Raleigh, NC. These two lines were tested extensively by the North Carolina Agricultural Research Service from 2006 through 2012 in disease evaluation tests. They have unique alleles introgressed from the diploid (2n = 2x = 20) wild species, A. cardenasii Krapov. & W.C. Gregory. The germplasm lines are also unique in that they exhibited multiple disease resistances superior to the germplasm lines derived from A. cardenasii that were released previously by NCSU. Resistance to multiple diseases included early leaf spot (ELS), Cylindrocladium black rot (CBR), Sclerotinia blight (SB), and tomato spotted wilt (TSW). One of the lines, GP-NC WS 17, also exhibited drought tolerance in field and greenhouse studies. Thus, it can be concluded that these two peanut germplasm lines derived from diploid wild species have multiple biotic stress resistances, specifically for ELS, CBR, SB, and TSWV, as well as abiotic stress resistance in the case of GP-NC WS 17. These two lines should provide unique, improved germplasm for breeders interested in multiple disease resistance and in expanding the germplasm pool of A. hypogaea.}, number={1}, journal={JOURNAL OF PLANT REGISTRATIONS}, author={Tallury, S. P. and Isleib, T. G. and Copeland, S. C. and Rosas-Anderson, P. and Balota, M. and Singh, D. and Stalker, H. T.}, year={2014}, month={Jan}, pages={86–89} }
 @article{shekoofa_devi_sinclair_holbrook_isleib_2013, title={Divergence in Drought-resistance Traits among Parents of Recombinant Peanut Inbred Lines}, volume={53}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.03.0153}, abstractNote={ABSTRACT}, number={6}, journal={CROP SCIENCE}, author={Shekoofa, Avat and Devi, J. Mura and Sinclair, Thomas R. and Holbrook, Corley C. and Isleib, Thomas G.}, year={2013}, pages={2569–2576} }
 @article{mothapo_grossman_maul_shi_isleib_2013, title={Genetic diversity of resident soil rhizobia isolated from nodules of distinct hairy vetch (Vicia villosa Roth) genotypes}, volume={64}, ISSN={["1873-0272"]}, DOI={10.1016/j.apsoil.2012.12.010}, abstractNote={Hairy vetch (Vicia villosa Roth, HV) is widely grown as a legume cover crop throughout the U.S.A., with biological nitrogen fixation (BNF) through symbiosis with Rhizobium leguminosarum biovar viciae (Rlv) being one of the most sought after benefits of its cultivation. This study determined if HV cultivation history and plant genotype affect genetic diversity of resident Rlv. Soil samples were collected from within farmers’ fields at Graham, Cedar Grove and Ivanhoe sites in North Carolina and pairs of genetically similar hairy vetch genotypes used as trap hosts. A total of 519 Rlv strains were isolated from six paired field soils, three with and three without histories of HV cultivation. A total of 46 strains failed to PCR-amplify the nifH gene; however nodC PCR amplification of these nifH-negative strains resulted in amplification of 22 of the strains. Repetitive element polymerase chain reaction (rep-PCR) with BOX-A1R primer and redundancy analysis showed rhizobial diversity to vary greatly within and between fields, with over 30 BOX banding patterns obtained across the six fields. Cluster analysis of BOX-PCR banding patterns resulted in 36 genetic groups of Rlv at a similarity level of 70%, with 15 of the isolates from fields with HV history not belonging to any of the clusters. Site was found to be the main driver of isolate diversity overall, explaining 57%, of the total variation among rhizobia occupying HV nodules, followed by history of hairy vetch cultivation. Evidence of a HV host genotype influence on the populations of rhizobia that infect hairy vetch was also observed, with plant genotype explaining 12.7% of the variation among all isolates. Our results show that second to site, HV cultivation history was the most important driver of rhizobial nodule community structure and increases the genetic diversity of resident Rlv in soils.}, journal={APPLIED SOIL ECOLOGY}, author={Mothapo, N. V. and Grossman, J. M. and Maul, J. E. and Shi, W. and Isleib, T.}, year={2013}, month={Feb}, pages={201–213} }
 @article{barkley_isleib_wang_pittman_2013, title={Genotypic effect of ahFAD2 on fatty acid profiles in six segregating peanut (Arachis hypogaea L) populations}, volume={14}, ISSN={["1471-2156"]}, DOI={10.1186/1471-2156-14-62}, abstractNote={Fatty acid composition of oil extracted from peanut (Arachis hypogaea L.) seed is an important quality trait because it may affect the flavor and shelf life of resulting food products. In particular, a high ratio of oleic (C18:1) relative to linoleic (C18:2) fatty acid (O/L ≥ 10) results in a longer shelf life. Previous reports suggest that the high oleic (~80%) trait was controlled by recessive alleles of ahFAD2A and ahFAD2B, the former of which is thought to have a high frequency in US runner- and virginia-type cultivars. Functional mutations, G448A in ahFAD2A and 442insA in ahFAD2B eliminate or knock down desaturase activity and have been demonstrated to produce peanut oil with high O/L ratios. In order to employ marker assisted selection (MAS) to select a high oleic disease resistant peanut and to evaluate genotypic and phenotypic variation, crosses were made between high oleic (~80%) and normal oleic (~50%) peanuts to produce segregating populations.A total of 539 F2 progenies were randomly selected to empirically determine each ahFAD2 genotype and the resulting fatty acid composition. Five of the six crosses segregated for the high oleic trait in a digenic fashion. The remaining cross was consistent with monogenic segregation because both parental genotypes were fixed for the ahFAD2A mutation. Segregation distortion was significant in ahFAD2A in one cross; however, the remaining crosses showed no distortion. Quantitative analyses revealed that dominance was incomplete for the wild type allele of ahFAD2, and both loci showed significant additive effects. Oleic and linoleic acid displayed five unique phenotypes, based on the number of ahFAD2 mutant alleles. Further, the ahFAD2 loci did exhibit pleiotropic interactions with palmitic (C16:0), oleic (C18:1), linoleic (C18:2) acids and the O/L ratio. Fatty acid levels in these progeny were affected by the parental genotype suggesting that other genes also influence fatty acid composition in peanut. As far as the authors are aware, this is the first study in which all of the nine possible ahFAD2 genotypes were quantitatively measured.The inheritance of the high oleic trait initially was suggested to be controlled by dominant gene action from two homoeologous genes (ahFAD2A and ahFAD2B) exhibiting complete recessivity. Analyzing the ahFAD2 genotypes and fatty acid compositions of these segregating peanut populations clearly demonstrated that the fatty acid contents are quantitative in nature although much of the variability in the predominant fatty acids (oleic, linoleic, and palmitic) is controlled by only two loci.}, journal={BMC GENETICS}, author={Barkley, Noelle A. and Isleib, Thomas G. and Wang, Ming Li and Pittman, Roy N.}, year={2013}, month={Jul} }
 @article{hurlburt_schmitt_isleib_cheng_garvey_koenig_maleki_2011, title={Production of pure protein and antibodies and development of immunoassays to detect Ara h 3 levels in peanut varieties}, volume={46}, ISSN={["0950-5423"]}, DOI={10.1111/j.1365-2621.2011.02645.x}, abstractNote={Summary}, number={7}, journal={INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY}, author={Hurlburt, Barry K. and Schmitt, David and Isleib, Thomas G. and Cheng, Hsiaopo and Garvey, Cathryn and Koenig, Robbin L. and Maleki, Soheila J.}, year={2011}, month={Jul}, pages={1477–1484} }
 @article{milla-lewis_zuleta_isleib_2010, title={Assessment of Genetic Diversity among U.S. Runner-Type Peanut Cultivars Using Simple Sequence Repeat Markers}, volume={50}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2010.04.0223}, abstractNote={The scientific community has long assumed that plant breeding activities decrease genetic diversity in crop species. To determine the influence of plant breeding on peanut, this study was designed to assess allelic diversity changes among peanut (Arachis hypogaea L.) cultivars of the runner market type using simple sequence repeat (SSR) markers. All runner‐type cultivars released to date were included with the exception of ten cultivars released in the 2000s. Thirty‐four SSR primer pairs amplified a total of 154 alleles. The results indicated that (i) at the gene level, allelic diversity has increased significantly through decades of breeding, (ii) at the population level, genetic diversity was at its lowest during the pre‐1980s time period and gradually increased in each subsequent decade, and (iii) most of the observed SSR variation occurred within, rather than among time periods. A principal coordinate analysis (PCO) clearly demonstrated increases in the variation present in each subsequent breeding decade, reaching its maximum in the 2000s. Therefore, it appears that runner‐type peanut breeders have been successful at developing improved peanut cultivars while increasing levels of diversity in the last three decades of breeding. In addition, genetic relationships among cultivars reported in this study might be of use for peanut breeders when selecting parents for establishment of breeding populations.}, number={6}, journal={CROP SCIENCE}, publisher={Crop Science Society of America}, author={Milla-Lewis, Susana R. and Zuleta, M. Carolina and Isleib, T. G.}, year={2010}, pages={2396–2405} }
 @article{isleib_milla-lewis_pattee_copeland_zuleta_shew_hollowell_sanders_dean_hendrix_et al._2010, title={Registration of ‘Bailey’ peanut}, volume={5}, ISSN={["1940-3496"]}, DOI={10.3198/jpr2009.12.0742crc}, abstractNote={‘Bailey’ (Reg. No. CV‐111, PI 659502) is a large‐seeded virginia‐type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) with partial resistance to five diseases that occur commonly in the Virginia‐Carolina production area: early leaf spot (caused by Cercospora arachidicola Hori), late leaf spot [caused by Cercosporidium personatum (Berk. & M.A. Curtis) Deighton], Cylindrocladium black rot [caused by Cylindrocladium parasiticum Crous, M.J. Wingf. & Alfenas], Sclerotinia blight (caused by Sclerotinia minor Jagger), and tomato spotted wilt (caused by Tomato spotted wilt tospovirus). It also has partial resistance to southern stem rot (caused by Sclerotium rolfsii Sacc.). Bailey was developed as part of a program of selection for multiple‐disease resistance funded by growers, seedsmen, shellers, and processors. Bailey was tested under the experimental designation N03081T and was released by the North Carolina Agricultural Research Service (NCARS) in 2008. Bailey was tested by the NCARS, the Virginia Agricultural Experimental Station, and five other state agricultural experiment stations and the USDA‐ARS units participating in the Uniform Peanut Performance Tests. Bailey has an alternate branching pattern, an intermediate runner growth habit, medium green foliage, and high contents of fancy pods and medium virginia‐type seeds. It has approximately 34% jumbo and 46% fancy pods, seeds with tan testas and an average weight of 823 mg seed−1, and an extra large kernel content of approximately 42%. Bailey is named in honor of the late Dr. Jack E. Bailey, formerly the peanut breeding project's collaborating plant pathologist.}, number={1}, journal={J. Plant Reg.}, publisher={American Society of Agronomy}, author={Isleib, T.G. and Milla-Lewis, S.R. and Pattee, H.E. and Copeland, S.C. and Zuleta, M.C. and Shew, B.B. and Hollowell, J.E. and Sanders, T.H. and Dean, L.O. and Hendrix, K.W. and et al.}, year={2010}, pages={27–39} }
 @article{milla-lewis_zuleta_isleib_2010, title={Simple sequence repeat allelic diversity in virginia-type peanut cultivars released from 1943 to 2006}, volume={50}, DOI={10.2135/cropsci2009.09.0501}, abstractNote={Studies on genetic diversity in Arachis spp. using microsatellite markers have included few or no commercial cultivars among the genotypes analyzed. The primary objective of this investigation was to evaluate the utility of simple sequence repeat (SSR) markers for detecting molecular polymorphism among elite virginia‐type peanut germplasm. Within that context, we had a secondary objective of assessing the impact of decades of plant breeding on allelic diversity levels among virginia‐type peanut cultivars. All U.S. virginia‐type cultivated varieties (except four) released between 1943 and 2006 were genotyped at 39 microsatellite loci. A total of 171 alleles were amplified. Allelic frequencies ranged from 0.02 to 0.97, with an average of 0.27. Although no significant difference was observed for the number of alleles present between the initial and the most recent time periods, our results indicate that levels of diversity present in virginia‐type peanuts have fluctuated significantly since the 1940s and peaked during the 1970s. Our study demonstrates that microsatellite markers may be useful for detecting molecular variation among peanut cultivars. Moreover, this is the first report of using microsatellite markers to describe genetic diversity in a collection of cultivated varieties of peanut.}, number={4}, journal={Crop Science}, publisher={Crop Science Society of America}, author={Milla-Lewis, S. R. and Zuleta, M. C. and Isleib, T. G.}, year={2010}, pages={1348–1356} }
 @article{smith_garrison_hollowell_isleib_shew_2008, title={Evaluation of application timing and efficacy of the fungicides fluazinam. and boscalid for control of Sclerotinia blight of peanut}, volume={27}, ISSN={["0261-2194"]}, DOI={10.1016/j.cropro.2007.11.010}, abstractNote={Sclerotinia blight of peanut (Arachis hypogaea) is caused by the soilborne fungus Sclerotinia minor. Management of Sclerotinia blight of peanut requires an integrated approach that includes rotation with non-hosts, resistant cultivars, cultural practices, and fungicides. Greenhouse experiments compared fluazinam and boscalid and investigated pre- and post-inoculation applications of fungicide or no fungicide to control infections by S. minor. Significant reductions in successful infections in the greenhouse occurred when fungicide was applied prior to, or up to 2 d after, inoculation, but not when applied 4 d after inoculation. Field experiments were conducted from 2004 to 2006 to investigate the comparative efficacy of the fungicides fluazinam and boscalid using alternating sequences of those fungicides or no fungicide for each of three sprays per season. In the field, applications of fungicide that preceded the largest incremental increase in disease incidence provided the best control of disease or increased yield. In both the field and greenhouse studies boscalid performed marginally better than fluazinam. Disease advisories or intensive scouting should be used to determine when epidemics initiate so that a fungicide can be applied prior to infection.}, number={3-5}, journal={CROP PROTECTION}, author={Smith, D. L. and Garrison, M. C. and Hollowell, J. E. and Isleib, T. G. and Shew, B. B.}, year={2008}, pages={823–833} }
 @article{smith_hollowell_isleib_shew_2007, title={A site-specific, weather-based disease regression model for Sclerotinia blight of peanut}, volume={91}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS-91-11-1436}, abstractNote={ In North Carolina, losses due to Sclerotinia blight of peanut, caused by the fungus Sclerotinia minor, are an estimated 1 to 4 million dollars annually. In general, peanut (Arachis hypogaea) is very susceptible to Sclerotinia blight, but some partially resistant virginia-type cultivars are available. Up to three fungicide applications per season are necessary to maintain a healthy crop in years highly favorable for disease development. Improved prediction of epidemic initiation and identification of periods when fungicides are not required would increase fungicide efficiency and reduce production costs on resistant and susceptible cultivars. A Sclerotinia blight disease model was developed using regression strategies in an effort to describe the relationships between modeled environmental variables and disease increase. Changes in incremental disease incidence (% of newly infected plants of the total plant population per plot) for the 2002–2005 growing seasons were statistically transformed and described using 5-day moving averages of modeled site-specific weather variables (localized, mathematical estimations of weather data derived at a remote location) obtained from SkyBit (ZedX, Inc.). Variables in the regression to describe the Sclerotinia blight disease index included: mean relative humidity (linear and quadratic), mean soil temperature (quadratic), maximum air temperature (linear and quadratic), maximum relative humidity (linear and quadratic), minimum air temperature (linear and quadratic), minimum relative humidity (linear and quadratic), and minimum soil temperature (linear and quadratic). The model explained approximately 50% of the variability in Sclerotinia blight index over 4 years of field research in eight environments. The relationships between weather variables and Sclerotinia blight index were independent of host partial resistance. Linear regression models were used to describe progress of Sclerotinia blight on cultivars and breeding lines with varying levels of partial resistance. Resistance affected the rate of disease progress, but not disease onset. The results of this study will be used to develop site- and cultivar-specific spray advisories for Sclerotinia blight. }, number={11}, journal={PLANT DISEASE}, author={Smith, D. L. and Hollowell, J. E. and Isleib, T. G. and Shew, B. B.}, year={2007}, month={Nov}, pages={1436–1444} }
 @article{smith_hollowell_isleib_shew_2006, title={Analysis of factors that influence the epidemiology of Sclerotinia minor on peanut}, volume={90}, ISSN={["1943-7692"]}, DOI={10.1094/PD-90-1425}, abstractNote={ In North Carolina, sclerotia of Sclerotinia minor germinate myceliogenically to initiate infections on peanut. The effects of soil temperature and soil matric potential (ψM on germination and growth of S. minor have not been well characterized, and little is known about relative physiological resistance in different parts of the peanut plant. Laboratory tests examined the ability of the fungus to germinate, grow, and infect detached peanut leaflets at soil temperatures ranging from 18 to 30°C at ψM of -100, -10, and -7.2 kPa. In addition, detached pegs, leaves, main stems, and lateral branches from three peanut lines varying in field resistance were examined for resistance to infection by S. minor. Sclerotial germination was greatest at 30°C and ψM of -7.2 kPa. Final mycelial diameters decreased with decreasing ψM, whereas soil matric potential did not affect lesion development. Mycelial growth and leaflet lesion expansion were maximal at 18 or 22°C. Soil ψM did not affect leaflet infection and lesion expansion. Lesions were not observed on leaves incubated at temperatures of 29°C or above, but developed when temperatures were reduced to 18 or 22°C 2 days after inoculation. Pegs and leaflets were equally susceptible to infection and were more susceptible than either main stems or lateral branches. Results of this work, particularly the effects of temperature on S. minor, and knowledge of peanut part susceptibility has application in improving Sclerotinia blight prediction models for recommending protective fungicide applications. }, number={11}, journal={PLANT DISEASE}, author={Smith, D. L. and Hollowell, J. E. and Isleib, T. G. and Shew, B. B.}, year={2006}, month={Nov}, pages={1425–1432} }
 @article{perkins_schmitt_isleib_cheng_maleki_2006, title={Breeding a Hypoallergenic Peanut}, volume={117}, ISSN={0091-6749}, url={http://dx.doi.org/10.1016/j.jaci.2005.12.1293}, DOI={10.1016/j.jaci.2005.12.1293}, abstractNote={RATIONALE: Approximately 700 varieties of peanut, including irradiated mutants, were previously screened to determine if variations could be found in the levels of allergenic proteins, Ara h 1, 2, and 3. Some irradiated peanuts were found to contain mutations resulting in the lack of one of the allergen isoforms or showed reduced levels of these proteins. These varieties were crossbred towards producing progeny missing more than one of the allergen isoforms. METHODS: The F2 generation produced by crossbreeding peanuts that were missing either an Ara h 2 or Ara h 3 isoform were homogenized and the proteins were extracted. The proteins were screened using anti-Ara h 2 and 3 antibodies and serum IgE from allergic individuals to determine if there were any peanuts that lacked isoforms of both Ara h 2 and Ara h 3. RESULTS: We observed that some of the crossbred peanuts were missing either an isoform of Ara h 3 or the higher molecular weight isoform of Ara h 2, and other peanuts were missing both. The observed numbers of double-mutant lines conformed to the 15:1 mendelian dihybrid ratio. CONCLUSIONS: Through conventional breeding practices it is possible to eliminate more than one allergenic protein, towards ultimately reducing sensitization ability and/or the severity of the allergic response to peanuts. Future studies will involve crossing these peanuts that lacked both isoforms with peanuts that have reduced levels of Ara h 1 or are missing other isoforms of Ara h 2 or Ara h 3.}, number={2}, journal={Journal of Allergy and Clinical Immunology}, publisher={Elsevier BV}, author={Perkins, T. and Schmitt, D.A. and Isleib, T.G. and Cheng, H. and Maleki, S.J.}, year={2006}, month={Feb}, pages={S328} }
 @article{isleib_pattee_sanders_hendrix_dean_2006, title={Compositional and sensory comparisons between normal- and high-oleic peanuts}, volume={54}, ISSN={["1520-5118"]}, DOI={10.1021/jf052353t}, abstractNote={The high-oleic-acid trait improves the oxidative stability of peanuts (Arachis hypogaea L.) and their products. The explicit effect of the trait on sensory quality, particularly on off-flavors associated with oil rancidity, has not been well documented. To assess the effect of the trait on off-flavors, data from two independent databases were analyzed to compare sensory quality and composition in normal- versus high-oleic peanut genotypes. In data collected using a sensory panel in the Department of Food Science at North Carolina State University, there were small differences between near-isogenic lines for intensities of the roasted peanut, astringent, over-roast, and nutty attributes, with the high-oleic lines exhibiting slightly greater intensities of those attributes. There were no differences for off-flavors such as fruity, painty, stale, moldy, or petroleum. In data collected from the multistate Uniform Peanut Performance Test and evaluated by a panel in the USDA-ARS Market Quality and Handling Research Unit (MQHRU) at Raleigh, NC, there were differences in chemical composition associated with the high-oleic trait, including differences in oil content, tocopherols, and carbohydrates in addition to the expected differences in fatty acid contents. There were small decreases in the intensities of the sensory attributes cardboard and painty associated with the high-oleic trait in the MQHRU data when all high-oleic lines were compared with all normal-oleic lines. Comparison of the near-isogenic pair NC 7 and N00090ol showed differences in oil and glucose contents, but not in sensory attributes. The high-oleic trait does not appear to have a major impact on sensory quality on average, although there were individual instances in which the trait was associated with shifts in sensory attribute intensities that may be perceptible to consumers.}, number={5}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Isleib, TG and Pattee, HE and Sanders, TH and Hendrix, KW and Dean, LO}, year={2006}, month={Mar}, pages={1759–1763} }
 @article{isleib_wilson_novitzky_2006, title={Partial dominance, pleiotropism, and epistasis in the inheritance of the high-oleate trait in peanut}, volume={46}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2005.09-0313}, abstractNote={ABSTRACT}, number={3}, journal={CROP SCIENCE}, author={Isleib, TG and Wilson, RF and Novitzky, WP}, year={2006}, pages={1331–1335} }
 @article{isleib_rice_mozingo_copeland_graeber_novitzky_pattee_sanders_mozingo_coker_2006, title={Registration of 'Brantley' peanut}, volume={46}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2005.12.0492}, abstractNote={‘Brantley’ (Reg. no. CV-86, PI 642026) is a large-seeded virginia-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) cultivar with high oleic fatty acid content in its seed oil, essentially derived from the ‘NC 7’ cultivar (Wynne et al., 1979). Brantley was tested under the experimental designation N00090ol and was released jointly by the North Carolina Agric. Res. Service (NCARS) and the USDA-ARS in 2005. Brantley was tested by the NCARS, by the Virginia Agric. Exp. Stn. (VAES), and five other state agricultural experiment stations participating in the Uniform Peanut Performance Tests (UPPT). Brantley is named in honor of the late Ms. Peggy Y. Brantley, long-time office administrator to the peanut breeding and genetics programs at North Carolina State Univ. (NCSU). Brantley is a virginia market type cultivar possessing alternate branching pattern, intermediate runner growth habit, medium green foliage, large seeds with tan testa averaging 895 mg seed, approximately 65% jumbo pods and 24% fancy pods, and extra large kernel content of approximately 50%. Brantley was developed by backcrossing the high-oleic trait patented by the University of Florida (Norden et al., 1987; Moore and Knauft, 1989; Knauft et al., 1993) into the NC 7 cultivar from Florida line F435 (Moore and Knauft, 1989). In the backcrossing steps, F4:5 progenies or individual F2 seeds were analyzed for fatty acid profiles using gas chromatography (Zeile et al., 1993), and high-oleic families or seeds were selected. BC4F2:3 families were grown in a replicated preliminary yield test in 1999. N00090ol was numbered in 2000 on entry into the NCSUAdvanced Yield Test series. Agronomic performance of Brantley has been evaluated in 16 trials conducted by the NCARS breeding program over 5 yr and 24 trials (including early and late diggings as separate trials) in the joint VAES-NCARS Peanut Variety and Quality Evaluation (PVQE) program over 3 yr (Coker and Mozingo, 2004, 2005). Brantley was also tested in the Uniform Peanut Performance Test series conducted at nine sites in seven states in 2003 (Branch et al., 2004). Because it was essentially derived from NC 7 by backcrossing, most characteristics of Brantley are similar to those of NC 7. The following comparisons are based on results from the PVQE program except as noted. Compared with NC 7, Brantley has similar pod yield (4479 vs. 4492 kg ha,ns), sound mature kernel content (67%), and meat content (73%), but more jumbo pods (64 vs. 58%, P , 0.05), fewer fancy size pods (24 vs. 29%, P, 0.05), more extra large kernels (ELK) (53 vs. 48%, P , 0.05), and greater jumbo pod brightness (44.5 vs. 43.5 Hunter L score, P , 0.01) (Isleib et al., 1997) and average pod brightness (43.9 vs. 43.0 Hunter L score, P , 0.01). The ratio of oleic to linoleic fatty acid of Brantley was greater than that of NC 7 (27.77 vs. 2.61, P , 0.01). In the NCSU trials, Brantley had greater brightness of jumbo pods than NC 7 (46.3 vs. 45.5 Hunter L score, P, 0.01), greater average pod brightness (45.1 vs. 44.4 Hunter L score, P , 0.05), and greater ELK content (46 vs. 43%, P , 0.01). Flavor attributes of roasted sound mature kernel samples from eight NCSU trials were evaluated by a trained sensory panel under the direction of USDA personnel. Averaged across eight samples taken from NCARS trials in 2000 and 2002 and adjusted to common values of roast color and fruity attribute (Pattee and Giesbrecht, 1990), the roasted peanut, sweet, bitter, and astringent attributes of flavor in Brantley were not different from those in NC 7, the flavor standard for the virginia market type. Averaged across ELK samples from nine UPPT locations from 2003 (USDA-ARS, 2004), Brantley was not significantly different from NC 7 for intensity of roasted peanut [4.41 vs. 4.61 flavor intensity units (fiu), ns], sweet (1.98 vs. 2.02 fiu, ns), or bitter (3.07 vs. 2.97 fiu, ns). Although it was not developed specifically to carry any particular disease resistance, Brantley was evaluated for resistance to diseases common to the Virginia-Carolina region. Because it was essentially derived from NC 7 by backcrossing, it was found to have the same susceptibilities to disease as NC 7. Brantley’s reaction to early leafspot (caused by Cercospora arachidicola S. Hori) was evaluated from 2001 through 2004 in four field trials with no application of leafspot fungicide during the entire season. Defoliation was rated on a proportional scale of 1 (no defoliation) to 9 (complete defoliation) in late September or early October each year, and yield was measured. Brantley was not significantly different from NC 7 in defoliation (7.0 vs. 6.8 defoliation score, ns) or yield (2380 vs. 2346 kg ha, ns). Brantley’s reactions to Cylindrocladium black rot (CBR) {caused by Cylindrocladium parasiticum Crous, Wingfield & Alfenas [syn. C. crotalariae (Loos) D.K. Bell & Sobers]} and to Sclerotinia blight (caused by Sclerotinia minor Jagger) were evaluated by the NCSU breeding project in four replicated tests on naturally infested soils with no chemical control of the soilborne diseases. Brantley was not different from NC 7 in incidence of CBR (37 vs. 33%, ns) or Sclerotinia blight (28 vs. 44%, ns). Brantley’s reaction to Tomato spotted wilt virus (TSWV) was evaluated from 2001 through 2004 in four field trials with seeds spaced 50 cm apart and no application of insecticides to control the thrips (Frankliniella fusca Hinds), the vector of the virus. Brantley was not different from NC 7 in incidence of TSWV symptoms (44 vs. 36%, ns). Like its recurrent parent NC 7, Brantley should be considered susceptible to all four of these diseases. Brantley is adapted to the Virginia–Carolina peanut production area but also has performed well in the southeastern US production area including Georgia, Florida, and Alabama. Breeder seed of Brantley will be maintained by the N.C. Agricultural Research Service, Box 7643, N.C. State University, Raleigh, NC 27695–7643. Foundation seed will be distributed by the N.C. Foundation Seed Producers, Inc., 8220 Riley Hill Rd., Zebulon, NC 27597. The N.C. Agricultural Research Service will provide small (50–100 seed) samples to research organizations for research purposes. An application is pending for protection of Brantley under the U.S. Plant Variety Protection Act as amended in 1994, under which Brantley may be sold only as a class of Certified seed. The high-oleic trait is protected by U.S. Patents (No. 5922,390, 6063,984, and 6121,472) issued to theUniv. of Florida by whose permission the trait was used.}, number={5}, journal={CROP SCIENCE}, author={Isleib, T. G. and Rice, P. W. and Mozingo, R. W., II and Copeland, S. C. and Graeber, J. B. and Novitzky, W. P. and Pattee, H. E. and Sanders, T. H. and Mozingo, R. W. and Coker, D. L.}, year={2006}, pages={2309–2311} }
 @article{isleib_rice_mozingo_copeland_graeber_pattee_sanders_mozingo_coker_2006, title={Registration of 'Phillips' peanut}, volume={46}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2005.12.0491}, abstractNote={‘Phillips’ (Reg. no. CV-85, PI 642025) is a large-seeded virginia-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) cultivar with a high percentage of bright fancy pods. Phillips was tested under the experimental designation N98003 and was released jointly by the North Carolina Agric. Res. Service (NCARS) and the USDA-ARS in 2005. Phillips was tested by theNCARS, by theVirginiaAgric. Exp. Stn. (VAES), and five other state agricultural experiment stations participating in the Uniform Peanut Performance Tests. Phillips is named for the late Ms. Ida G. “Gerry” Phillips, long-time research aide to the peanut breeding program at North Carolina State University (NCSU). Phillips is a virginia market type cultivar possessing alternate branching pattern, intermediate runner growth habit, medium green foliage, large seeds with tan testa averaging 835 mg seed, approximately 34% jumbo pods and 45% fancy pods. Phillips is an F5–derived line selected from a cross between two early maturing NCSU breeding lines, N90014E used as the female and N91024 used as the male. Both parents were F5–derived selections from a cross between ‘NC 7’ (Wynne et al., 1979) and ‘NC 9’ (Wynne et al., 1986). Singleseed descent was practiced in the F2 at the PeanutBelt Research Station (PBRS) in Bertie Co. near Lewiston, NC, during the summer of 1994 and in the F3 at a winter nursery at Juana Diaz, PR. The F1:4 family was subjected to selection for pod size and shape in the field at PBRS in 1995 and the F4:5 family in 1996. Yield and grade data were collected on F5:6 families in a replicated preliminary yield test in 1997. The F5:7 family was tested under the experimental designation N98003 in subsequent years. Agronomic performance of Phillips was evaluated in 16 trials conducted by the NCARS breeding program from 1998 through 2004 and 40 trials (including early and late diggings as separate trials) in the joint VAES-NCARS Peanut Variety and Quality Evaluation (PVQE) program from 2000 through 2004 (Coker andMozingo, 2004, 2005). Phillips was also tested in the Uniform Peanut Performance Test (UPPT) series conducted at nine sites in seven states in 2003 (Branch et al., 2004). The following comparisons are based on results from thePVQEprogramexcept as noted. Comparedwith ‘NC-V11’ (Wynne et al., 1991), Phillips had similar pod yield (5102 vs. 5014 kg ha, ns), and fancy pod content (45 vs. 47%, ns), but greater jumbo pod content (34 vs. 26%, P, 0.01), jumbo pod brightness (44.5 vs. 43.5 Hunter L score,P, 0.01) (Isleib et al., 1997), fancy pod brightness (45.8 vs. 44.9 Hunter L score, P , 0.01), average pod brightness (46.1 vs. 45.0 Hunter L score, P , 0.01), extra large kernel (ELK) content (47 vs. 34%, P , 0.01), soundmature kernel content (69 vs. 67%, P, 0.01), and meat content (74 vs. 73%, P, 0.01), and crop value at federal support price ($2095 vs. $1999 ha, P , 0.01). Although the ratio of oleic to linoleic fatty acid in seed oil of Phillips was greater than that of NC-V 11 (1.63 vs. 1.57, P , 0.05), both cultivars have oleic acid levels considered to be low within the normal range for virginia-type peanuts. In the NCSU trials, Phillips had greater brightness of fancy pods than NC-V 11 (46.9 vs. 45.3 Hunter L score, P , 0.01), greater average pod brightness (46.5 vs. 45.3 Hunter L score, P , 0.01), greater ELK content (43 vs. 33%, P, 0.01), and greater meat content (72 vs. 70%, P , 0.05). Flavor attributes of roasted sound mature kernel samples from three NCSU trials were evaluated by a trained sensory panel under the direction of USDA personnel. Adjusted to common values of roast color and fruity attribute (Pattee and Giesbrecht, 1990), intensity of the roasted peanut attribute of flavor in Phillips was not different from that in NC 7 [3.36 vs. 3.54 flavor intensity units (fiu), ns], the flavor standard for the virginia market-type, but intensity of the sweet attribute was higher (3.45 vs. 2.70 fiu, P , 0.05) and that of the bitter attribute lower 2.30 vs. 2.73 fiu, P , 0.05). Averaged across ELK samples from nine UPPT locations from 2003, Phillips was not significantly different from NC 7 for intensity of roasted peanut (4.55 vs. 4.61 fiu, ns), sweet (2.03 vs. 2.02 fiu, ns), or bitter (3.05 vs. 2.97 fiu, ns) (USDA, 2004). Although it was not developed specifically to carry any particular disease resistance, Phillips was evaluated for resistance to diseases common to the Virginia-Carolina region. Phillips’s reaction to early leafspot (caused by Cercospora arachidicola S. Hori) was evaluated from 1999 through 2004 in six field trials with no application of leafspot fungicide during the entire season. Defoliation was rated on a proportional scale of 1 (no defoliation) to 9 (complete defoliation) in late September or early October each year, and yield was measured on the unsprayed plots. Phillips was not significantly different from NCV 11 in defoliation (6.7 vs. 7.0 defoliation score, ns) or yield (2707 vs. 2563 kg ha, ns). Phillips’s reactions to Cylindrocladium black rot (CBR) {caused byCylindrocladium parasiticum Crous, Wingfield & Alfenas [syn. C. crotalariae (Loos) D.K. Bell & Sobers]} and to Sclerotinia blight (caused by Sclerotinia minor Jagger) were evaluated by the NCSU breeding project in six replicated tests on naturally infested soils with no chemical control of the soilborne diseases. Phillips was not different from NC-V 11 in incidence of CBR (27 vs. 23%, ns) or Sclerotinia blight (39 vs. 31%, ns). Phillips’s reaction to Tomato spotted wilt virus (TSWV) was evaluated from 1998 through 2004 in six field trials with seeds spaced 50 cm apart and no application of insecticides to control thrips (Frankliniella fusca Hinds), the vector of the virus. Phillips was not different from NC-V 11 in incidence of TSWV symptoms (35 vs. 30%, ns). Phillips should be considered susceptible to all four of these diseases. Phillips is adapted to the Virginia–Carolina peanut production area but also has performed well in the southeastern U.S. production area including Georgia, Florida, and Alabama. Breeder seed of Phillips will be maintained by the N.C. Agricultural Research Service, Box 7643, N.C. State University, Raleigh, NC 27695–7643. Foundation seed will be distributed by the N.C. Foundation Seed Producers, Inc., 8220 Riley Hill Rd., Zebulon, NC 27597. The N.C. Agricultural Research Service will provide small (50–100 seed) samples to research organizations for research purposes. An application for protection of Phillips under the U.S. Plant Variety Protection Act as amended in 1994 is pending. Phillips may be sold only as a class of Certified seed.}, number={5}, journal={CROP SCIENCE}, author={Isleib, T. G. and Rice, P. W. and Mozingo, R. W., II and Copeland, S. C. and Graeber, J. B. and Pattee, H. E. and Sanders, T. H. and Mozingo, R. W. and Coker, D. L.}, year={2006}, pages={2308–2309} }
 @article{isleib_rice_mozingo_copeland_graeber_shew_smith_melouk_stalker_2006, title={Registration of N96076L peanut germplasm line}, volume={46}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2005.12.0479}, abstractNote={N96076L (Reg. no. GP-125, PI 641950) is a large-seeded virginia-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) germplasm line with resistance to multiple diseases including early leafspot (caused by Cercospora arachidicola S. Hori), Cylindrocladium black rot (CBR) {caused by Cylindrocladium parasiticum Crous, Wingfield & Alfenas [syn. C. crotalariae (Loos)D.K.Bell&Sobers]}, Sclerotiniablight (caused by Sclerotinia minor Jagger), and tomato spotted wilt caused by Tomato spotted wilt virus (TSWV). N96076Lwas released by the NorthCarolinaAgriculturalResearchService (NCARS) in 2005. N96076Lwas tested by theNCARS, by the VirginiaAgricultural Experiment Station (VAES), and the USDA-ARSWheat, Peanut and Other Field Crops Research Unit at Stillwater, OK. N96076L is a virginia market-type line possessing alternate branching pattern, runner growth habit, medium green foliage, large seeds with dull tan testa averaging 880 mg seed, approximately 70% jumbo pods and 22% fancy pods. N96076L is an F4–derived line selected from cross X91053 made in 1991 using NCSU breeding line N90004 as the female and leafspotresistant germplasm line GP NCWS 13 (Stalker et al., 2002) as the male. N90004 was an F5–derived line selected from NCSU cross X84002 between ‘NC 7’ (Wynne et al., 1979) and ‘Florigiant’ (Carver, 1969). GP NC WS 13 has complex ancestry. One quarter of its ancestry comes from GP NC WS 4 (Stalker and Beute, 1993), a tetraploid (2n 5 4x 5 40) selection from a cross between PI 261942 (A. hypogaea subsp. fastigiataWaldron var. fastigiata) and leafspot-resistant diploid (2n5 2x5 20) wild species A. cardenasii Krapov. & W.C. Gregory GKP 10017 (PI 262141). One quarter of GP NC WS 13’s ancestry comes from leafspot-resistantA. hypogaea PI 270807, and one half from the cultivar ‘NC 5’ (Emery andGregory, 1970), which has moderate resistance to early leafspot. F1 plants of cross X91053 were grown at a winter nursery in Puerto Rico, single-seed descent was practiced in the F2 and F3 generations, and single-plant selections were made in the F4 generation. F4:5 families were planted at Peanut Belt Research Station (PBRS) at Lewiston in Bertie County, NC, in a field receiving no fungicide treatment to control leafspot in the summer of 1995. Families exhibiting reduced levels of defoliation were harvested in bulk and retained for evaluation in the Leafspot Test, a two-rep test of defoliation, yield, and grade grown without leafspot control at PBRS in 1996. Family X91053 F2-S-S-08: F05 was numbered N96076L when entered in the 1996 Leafspot Test. Although N96076L was developed for resistance to early leafspot, it also was evaluated for resistance to other diseases common to theVirginia–Carolina region.N96076L’s reaction to early leafspot was evaluated from 1996 through 2004 in 12 field trials with no application of leafspot fungicide during the entire season. Defoliation was rated on a proportional scale of 1 (no defoliation) to 9 (complete defoliation) in late September or early October each year, and yield was measured on the unsprayed plots. Although N96076L had more defoliation than resistant checkGP-NC343(Campbell etal., 1971) (5.5vs.4.3,P, 0.01), it had less than either ‘NC 12C’ (Isleib et al., 1997) (5.7 vs. 6.2 defoliation score,P, 0.01) or ‘Perry’ (Isleib et al., 2003) (5.8 vs. 6.6defoliation score,P,0.01), the twomost resistantvirginiatype cultivars. N96076L did not differ significantly from any of these three checks for yield in the absence of leafspot control. N96076L’s reactions to Cylindrocladium black rot (CBR) and to Sclerotinia blight were evaluated by the NCSU breeding project from 1997 through 2004 in eight replicated tests conducted in North Carolina on naturally infested soils with no chemical control of these diseases. N96076L was not significantly different from the resistant cultivar Perry in incidence of CBR (8 vs. 10%, ns), but it did have lower CBR incidence than NC 12C (9 vs. 21%, P , 0.01) and ‘Gregory’ (Isleib et al., 1999) (8 vs. 17%, P, 0.01). N96076L was not different from the partially resistant cultivar Perry in incidence of Sclerotinia blight (7 vs. 21%, ns), but it did have lower incidence than NC 12C (6 vs. 28%, P , 0.01) and Gregory (7 vs. 30%, P, 0.01). Yield, grade and Sclerotinia blight incidence in N96076L were evaluated by USDA-ARS personnel at Stillwater, OK, in a two-rep trial conducted in infested soil at Fort Cobb, OK, during 1998. Disease incidence in N96076L was less than in any of the lines tested except ‘Tamrun 98’ (Simpson et al., 2000) (16 vs. 30%, ns), but there was no variation in yield among the lines tested. Physiological resistance to S. minor was documented in detached plant part inoculations under controlled laboratory conditions (Smith, 2004, p. 72–93). Lesion development measured by the area under the disease progress curve (AUDPC) was significantly smaller for all parts with the exception of mainstems when compared to NC 12C and NC 7 (P , 0.0001). In the field, resistance most likely due to avoidance was also documented. Fewer infections were detected on lateral branches of N96076L plants when compared with NC 12C (13 vs. 46%, P , 0.01), Perry, (13 vs. 44%, P , 0.01), and ‘VA 98R’ (Mozingo et al., 2000) (13 vs. 23%, P , 0.01). N96076L’s reaction to TSWV was evaluated from 1997 through 2004 in 18 field trials with seeds spaced 50 cm apart and no application of insecticides to control thrips (Frankliniella fusca Hinds), the vector of the virus. N96076L had lower incidence of TSWV symptoms than NC 12C (22 vs. 45%,P, 0.01), Gregory (26 vs. 33%,P, 0.01), and Perry (25 vs. 52%,P, 0.01) and was not different from resistant check PI 576636 (21 vs. 16%, ns). N96076L should be considered resistant to all four of these diseases. Agronomic performance of N96076L was evaluated in 13 trials conducted by the NCARS breeding program over 1996 to 2004. Although yield of N96076L was not significantly different from that of NC 12C (3774 vs. 4050 kg ha, ns), Gregory (3703 vs. 3960 kg ha, ns) or Perry (3702 vs. 3709 kg ha, ns), its average pod brightness (42.7 Hunter L score) (Isleib et al., 1997) was less (44.6 for NC 12C, P, 0.01; 44.3 for Gregory, P, 0.01; and 44.4 for Perry, P, 0.01), making N96076L unsuitable for use as a cultivar for the in-shell market. N96076L is adapted to the Virginia-Carolina peanut production area. Seed of N96076L will be maintained by the N.C. Agricultural Research Service, Box 7643, N.C. State University, Raleigh, NC 27695–7643. Foundation seed will be distributed by the N.C. Foundation Seed Producers, Inc., 8220 Riley Hill Rd., Zebulon, NC 27597. The N.C. Agricultural Research Service will provide small (50–100 seed) samples to research organizations for research purposes.}, number={5}, journal={CROP SCIENCE}, author={Isleib, T. G. and Rice, P. W. and Mozingo, R. W., II and Copeland, S. C. and Graeber, J. B. and Shew, B. B. and Smith, D. L. and Melouk, H. A. and Stalker, H. T.}, year={2006}, pages={2329–2330} }
 @article{milla_isleib_stalker_2005, title={Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers}, volume={48}, ISSN={["1480-3321"]}, DOI={10.1139/g04-089}, abstractNote={ Cultivated peanut, Arachis hypogaea L., is a tetraploid (2n = 4x = 40) species thought to be of allopolyploid origin. Its closest relatives are the diploid (2n = 2x = 20) annual and perennial species included with it in Arachis sect. Arachis. Species in section Arachis represent an important source of novel alleles for improvement of cultivated peanut. A better understanding of the level of speciation and taxonomic relationships between taxa within section Arachis is a prerequisite to the effective use of this secondary gene pool in peanut breeding programs. The AFLP technique was used to determine intra- and interspecific relationships among and within 108 accessions of 26 species of this section. A total of 1328 fragments were generated with 8 primer combinations. From those, 239 bands ranging in size from 65 to 760 bp were scored as binary data. Genetic distances among accessions ranged from 0 to 0.50. Average distances among diploid species (0.30) were much higher than that detected between tetraploid species (0.05). Cluster analysis using different methods and principal component analysis were performed. The resulting grouping of accessions and species supports previous taxonomic classifications and genome designations. Based on genetic distances and cluster analysis, A-genome accessions KG 30029 (Arachis helodes) and KSSc 36009 (Arachis simpsonii) and B-genome accession KGBSPSc 30076 (A. ipaensis) were the most closely related to both Arachis hypogaea and Arachis monticola. This finding suggests their involvement in the evolution of the tetraploid peanut species.Key words: peanut, numerical taxonomy, genome donors, classification. }, number={1}, journal={Genome}, publisher={Canadian Science Publishing}, author={Milla, S.R. and Isleib, T.G. and Stalker, H.T.}, year={2005}, pages={1–11} }
 @article{isleib_pattee_giesbrecht_2004, title={Oil, sugar, and starch characteristics in peanut breeding lines selected for low and high oil content and their combining ability}, volume={52}, ISSN={["1520-5118"]}, DOI={10.1021/jf035465y}, abstractNote={Peanut seeds contain approximately 50% oil on a dry weight basis, making them a high fat food. Reduction of the oil content would make peanuts a more desirable food to fat conscious consumers. Removal of existing oil by processing is not feasible for in-shell peanuts, the dominant product of the North Carolina-Virginia area. To reduce oil content in in-shell peanuts, a genetic solution must be found. However, while reduced oil content is a desirable objective, changes in oil must not be accompanied by significant decreases in any of the desirable aspects of peanut flavor. Because the impact of selection for low or high oil on flavor is not known, it would be useful to know in what form dry matter is being stored in the seed, particularly if it is not being stored as oil. Screening of 584 accessions identified two lines (PI 269723 and PI 315608) with high and two (Robusto 2 and Robusto 3) with low oil contents, each pair differing in sugar content. The four parents were crossed in diallel fashion to investigate patterns of inheritance. General combining abilities (GCA) for oil content closely followed values of the parental lines. One low oil parent (Robusto 2) had a correspondingly elevated GCA for sugar content, but neither low oil parent had the effect of elevating starch in progeny. Reciprocal cross differences were found for starch and sugar contents, suggesting influences of cytoplasmic genes on those traits. These lines serve as resource material for researchers interested in the genetic and physiological aspects of the oil-sugar-starch relationship in peanuts.}, number={10}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Isleib, TG and Pattee, HE and Giesbrecht, FG}, year={2004}, month={May}, pages={3165–3168} }
 @article{xue_isleib_payne_wilson_novitzky_g o'brian_2003, title={Comparison of aflatoxin production in normal- and high-oleic backeross-derived peanut lines}, volume={87}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.2003.87.11.1360}, abstractNote={ The effect of the high-oleate trait of peanut on aflatoxin production was tested by comparing normal oleic lines with high-oleic backcross-derived lines. Seeds were blanched, quartered, and inoculated with Aspergillus flavus conidia, placed on moistened filter paper in petri dishes, and incubated for 8 days. In one experiment, dishes were stacked in plastic bags in a Latin square design with bags and positions in stacks as blocking variables. High-oleic lines averaged nearly twice as much aflatoxin as normal lines. Background genotype had no significant effect on aflatoxin content, and interaction between background genotype and oleate level was not detected. In a second experiment, dishes were arranged on plastic trays enclosed in plastic bags and stacked with PVC spacers between trays. Fungal growth and aflatoxin production were greater than in the first experiment. Background genotype, oleate level, and their interaction were significant. The mean of high-oleic lines was almost twice that of normal lines, but the magnitude of the difference varied with background genotype. Special care should be taken with high-oleic lines to prevent growth of Aspergillus spp. and concomitant development of aflatoxin contamination. }, number={11}, journal={PLANT DISEASE}, author={Xue, HQ and Isleib, TG and Payne, GA and Wilson, RF and Novitzky, WP and G O'Brian}, year={2003}, month={Nov}, pages={1360–1365} }
 @article{hollowell_shew_isleib_2003, title={Evaluating isolate aggressiveness and host resistance from peanut leaflet inoculations with Sclerotinia minor}, volume={87}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.2003.87.4.402}, abstractNote={ Sclerotinia minor is a major pathogen of peanut in North Carolina, Virginia, Oklahoma, and Texas. Partial resistance to S. minor has been reported based on field screening, but field performance is not always correlated with laboratory or greenhouse evaluations of resistance. More efficient screening methods and better understanding of the mechanisms contributing to Sclerotinia blight resistance are needed, and a detached leaf assay was developed and evaluated. Detached leaflets of 12 greenhouse-grown peanut lines were inoculated on the adaxial surface with a 4-mm-diameter mycelial plug of a single isolate of S. minor. Leaflets were incubated in the dark at 20°C in Nalgene utility boxes containing moistened sand. Lesion length 3 days after inoculation ranged from 11 to 24 mm, with a mean of 19 mm. Lengths differed significantly among the entries, with GP-NC WS 12, an advanced breeding line derived from a cross of NC 6 × (NC 3033 × GP-NC WS 1), being the most resistant. Forty-eight isolates of S. minor obtained from peanut were inoculated on leaflets of the susceptible cultivar NC 7 and aggressiveness was assessed by measuring lesion-length expansion. Three days after inoculation, lesion length differed among the isolates and ranged from 2 to 24 mm, with a mean of 15 mm. Finally, the potential for specific interactions between peanut lines and S. minor isolates was evaluated. A subset of S. minor isolates was selected to represent the observed range of aggressiveness and a subset of peanut entries was selected to represent the range of resistance or susceptibility. Nine-week-old greenhouse- or field-grown plants were compared for five peanut entries. Main effects of isolates and entries were highly significant, but isolate-entry interactions were not significant. The most resistant peanut entry (GP-NC WS 12) performed consistently with all isolates regardless of plant source. }, number={4}, journal={PLANT DISEASE}, author={Hollowell, JE and Shew, BB and Isleib, TG}, year={2003}, month={Apr}, pages={402–406} }
 @article{isleib_rice_mozingo_bailey_mozingo_pattee_2003, title={Registration of 'Perry' peanut}, volume={43}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2003.0739}, abstractNote={Crop ScienceVolume 43, Issue 2 p. 739-740 Registrations of Cultivar Registration of ‘Perry’ Peanut T.G. Isleib, Corresponding Author T.G. Isleib [email protected] Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Corresponding author ([email protected])Search for more papers by this authorP.W. Rice, P.W. Rice Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorR.W. Mozingo, R.W. Mozingo Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629 Dep. of Soil and Environ. Sciences, Tidewater Agric. Res. Ext. Center, 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorJ.E. Bailey, J.E. Bailey Dep. of Plant Pathology, Box 7616, N.C. State Univ., Raleigh, NC, 27695-7616Search for more papers by this authorR.W. Mozingo, R.W. Mozingo Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629 Dep. of Soil and Environ. Sciences, Tidewater Agric. Res. Ext. Center, 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorH.E. Pattee, H.E. Pattee USDA-ARS, Box 7625, N.C. State Univ., Raleigh, NC, 27695-7625Search for more papers by this author T.G. Isleib, Corresponding Author T.G. Isleib [email protected] Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Corresponding author ([email protected])Search for more papers by this authorP.W. Rice, P.W. Rice Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorR.W. Mozingo, R.W. Mozingo Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629 Dep. of Soil and Environ. Sciences, Tidewater Agric. Res. Ext. Center, 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorJ.E. Bailey, J.E. Bailey Dep. of Plant Pathology, Box 7616, N.C. State Univ., Raleigh, NC, 27695-7616Search for more papers by this authorR.W. Mozingo, R.W. Mozingo Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629 Dep. of Soil and Environ. Sciences, Tidewater Agric. Res. Ext. Center, 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorH.E. Pattee, H.E. Pattee USDA-ARS, Box 7625, N.C. State Univ., Raleigh, NC, 27695-7625Search for more papers by this author First published: 01 March 2003 https://doi.org/10.2135/cropsci2003.7390Citations: 14 Partial support for the development of NC 12C was provided by the N.C. Peanut Growers' Association, Inc., the N.C. Crop Improvement Association, the N.C. Foundation Seed Producers, Inc., and the USAID Peanut Collaborative Research Support Program. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL References Beute M.K., Registration of NC 3033 peanut germplasm (Reg. No. GP 9). :. Crop Sci. (1976) 16, 887http://doi.org/10.2135/cropsci1976.0011183X001600060046x Carver W.A., Registration of Florigiant peanuts (Reg. No. 1). Crop Sci. (1969) 9, 849– 850 http://doi.org/10.2135/cropsci1969.0011183X000900060067x, Coffelt T.A., Registration of ‘VA 93B’ peanut. :. Crop Sci. (1994) 34, 1126http://doi.org/10.2135/cropsci1994.0011183X003400040060x, Gregory W.C., Registration of NC 2 peanuts (Reg. No. 5). Crop Sci. (1970) 10, 459– 460 http://doi.org/10.2135/cropsci1970.0011183X001000040058x Isleib T.G., a. Registration of ‘NC 12C’ peanut. :. Crop Sci. (1997) 37, 1976http://doi.org/10.2135/cropsci1997.0011183X003700060051x, Isleib T.G., b. Use of a laboratory colorimeter to measure pod brightness in virginia-type peanuts. Peanut Sci. (1997) 24, 81– 84 http://doi.org/10.3146/i0095-3679-24-2-4 Mozingo R.W. 1999. Peanut variety and quality evaluation results, 1999. I. Agronomic and grade data. Virginia Polytech. Inst. and State Univ. Inf. Ser. No. 422. Mozingo R.W. 2000. Peanut variety and quality evaluation results, 1999. II. Quality data. Virginia Polytech. Inst. and State Univ. Inf. Ser. No. 423. Mozingo R.W., Registration of VA 98R peanut. Crop Sci. (2000) 40, 1202– 1203 http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=agrocropsoil&KeyUT=000089410600060&DestLinkType=FullRecord&DestApp=WOS_CPL&UsrCustomerID=523bbf5d2a868de7bbaeea0bc70ec0e4 Pattee H.E., Adjusting roast peanut scores for fruity attribute and non-optimum CIELAB L* values. J. Sens. Stud. (1990) 9, 353– 363 http://doi.org/10.1111/j.1745-459X.1994.tb00253.x Wynne J.C., Registration of ‘NC-V11’ peanut. Crop Sci. (1991) 31, 484– 485 http://doi.org/10.2135/cropsci1991.0011183X003100020062x, Wynne J.C., Registration of NC 7 peanut (Reg. No. 22). :. Crop Sci. (1979) 19, 563http://doi.org/10.2135/cropsci1979.0011183X001900040037x, Wynne J.C., Registration of ‘NC 9’ peanut. :. Crop Sci. (1986) 26, 197http://doi.org/10.2135/cropsci1986.0011183X002600010051x Citing Literature Volume43, Issue2March–April 2003Pages 739-740 ReferencesRelatedInformation}, number={2}, journal={CROP SCIENCE}, author={Isleib, TG and Rice, PW and Mozingo, RW and Bailey, JE and Mozingo, RW and Pattee, HE}, year={2003}, pages={739–740} }
 @article{pattee_isleib_moore_gorbet_giesbrecht_2002, title={Effect of high-oleic trait and paste storage variables on sensory attribute stability of roasted peanuts}, volume={50}, ISSN={["0021-8561"]}, DOI={10.1021/jf025853k}, abstractNote={There has been much interest in the effect of the high-oleic acid trait of peanuts on various quality factors since discovery of high levels of oleic acid in a peanut mutant genotype. The trait provides greater oxidative stability for the high-oleic oil and seed. Several research groups have investigated high-oleic peanut oil and roasted peanut flavor characteristics, which were similar within high-oleic lines compared to Florunner. It was observed that some high-oleic lines derived from the Sunrunner cultivar have consistently higher predicted breeding values for roasted peanut attribute than Sunrunner itself. This study investigated if this apparent effect of the trait was an artifact arising from the handling procedures during processing and storage or from flavor fade. High-oleic lines used were derived by backcrossing the trait into existing cultivars, and the comparison of sensory attribute intensity was with the recurrent parent used in backcrossing. Previous comparisons have been between lines differing in more than just oleate content, that is, with widely different background genotypes that could contribute to the differences observed. Differential rates of change in sensory attributes were found in different background genotypes, suggesting that the comparison of high- and normal-oleic lines should be made in common background genotypes as well as in common production and postharvest environments. There was no measurable change in roasted peanut attribute in samples stored at -20 degrees C over the 63 day duration of this experiment. There were changes in roasted peanut in samples stored at 22 degrees C, confirming that storage at -20 degrees C is sufficient for large studies that require multiple sensory panel sessions over a period of weeks.}, number={25}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Pattee, HE and Isleib, TG and Moore, KM and Gorbet, DW and Giesbrecht, FG}, year={2002}, month={Dec}, pages={7366–7370} }
 @article{pattee_isleib_gorbet_moore_lopez_baring_simpson_2002, title={Effect of the high-oleic trait on roasted peanut flavor in backcross-derived breeding lines}, volume={50}, ISSN={["0021-8561"]}, DOI={10.1021/jf025854c}, abstractNote={The high-oleic trait of peanut (Arachis hypogaea L.) has been suggested to have a positive impact on the roasted peanut sensory attribute. A series of lines derived by backcrossing the high-oleic trait into several existing cultivars were compared with their parent cultivars at locations in Florida, Georgia, North Carolina, and Texas. Breeders grew their high-oleic lines and parents in three-replicate tests at one or two locations. The Florida high-oleic line F435-2-3-B-2-1-b4-B-B-3-b3-b3-1-B was grown at each location. The test included normal- and high-oleic variants of F435, GK 7, NC 7, NC 9, Sunrunner, Tamrun 96, and Tamspan 90. Sound-mature kernel samples were roasted, ground into paste, and evaluated by a sensory panel using a 14-point flavor intensity unit (fiu) scale. Background genotype had an effect (P < 0.01) on the heritable sensory attributes roasted peanut, sweet, and bitter. Oleate level had a positive effect on roasted peanut intensity, increasing it by 0.3 fiu averaged across all seven background genotypes. However, the magnitude of improvement varied across background genotypes. The high-oleic trait had no effect or increased the intensity of the roasted peanut attribute in each background genotype. The increase was greatest in Tamrun 96 (+0.6 fiu, P < 0.05) and Spanish genotypes Tamspan 90 (+0.4 fiu, P < 0.05) and F435 (+0.4 fiu, P < 0.10). A change of 0.5 fiu or more should be perceptible to consumers. Interaction between oleate level and background genotype was detected for sweet (P < 0.10) and bitter (P < 0.01) attributes. The trait had an increasing effect on the bitter attribute only in the background genotype of Tamspan 90 (+0.7 fiu, P < 0.01). There was a nonsignificant increase in bitterness in the other Spanish background genotype, F435. Changes in bitterness in runner- and Virginia-type backgrounds were close to zero. Incorporation of the high-oleic trait into peanut cultivars is likely to improve the intensity of the roasted peanut attribute, but it may also increase the bitter attribute in Spanish genotypes.}, number={25}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Pattee, HE and Isleib, TG and Gorbet, DW and Moore, KM and Lopez, Y and Baring, MR and Simpson, CE}, year={2002}, month={Dec}, pages={7362–7365} }
 @article{beam_jordan_york_isleib_bailey_mckemie_spears_johnson_2002, title={Influence of prohexadione calcium on pod yield and pod loss of peanut}, volume={94}, DOI={10.2134/agronj2002.9550}, abstractNote={Excessive vegetative growth of peanut (Arachis hypogaea L.) can make digging and inverting operations less efficient. Reducing vine growth by applying a suitable plant growth regulator would be an efficient way to manage peanut vines. Pod yield, market grade factors, and gross economic value of peanut treated with prohexadione calcium (calcium salt of 3,5-dioxo-4 propionylcyclohexanecarboxylic acid) were evaluated at 19 sites in North Carolina during 1999 and 2000. Experiments were also conducted at two locations each during 1999 and 2000 to determine the effect of prohexadione Ca, digging date, and lifting (shaking peanut vines after digging to remove soil before combining) on combined yield, market grade factors, gross economic value, seed germination, and pod loss of the virginia market-type cultivar NC 12C. Prohexadione Ca at 140 g a.i. ha -1 , applied at 50% row closure and repeated 2 wk later, increased row visibility at harvest, pod yield by 310 kg ha -1 , and gross economic value of quota peanut by $223 ha -1 when pooled over 19 sites. Prohexadione Ca increased combined yield by 220 kg ha -1 and decreased percent pod loss by 4% regardless of digging date and lifting treatment compared with nontreated peanut. Prohexadione Ca did not affect maximum yield (sum of pods remaining in soil and on the soil surface and pods that were combined) or germination of peanut seed. These data suggest that increased combined yield noted following application of prohexadione Ca can be partially attributed to decreased pod loss.}, number={2}, journal={Agronomy Journal}, author={Beam, J. B. and Jordan, D. L. and York, A. C. and Isleib, T. G. and Bailey, J. E. and McKemie, T. E. and Spears, J. F. and Johnson, P. D.}, year={2002}, pages={331–336} }
 @article{pattee_isleib_giesbrecht_cui_2002, title={Prediction of parental genetic compatibility to enhance flavor attributes of peanuts}, volume={829}, DOI={10.1021/bk-2002-0829.ch017}, abstractNote={As future advances in transformation technology allow insertion of useful genes into a broader array of target genotypes, the choice of targets will become more important. Targets should be genotypes that will pass to their progeny other useful characteristics, such as sensory quality characteristics, while improving agronomic performance or pest resistance. This is particularly important if flavor quality is to be maintained or improved as the transgene is moved into breeding populations via sexual transfer. Selection of genotypes with superior breeding values through the use of Best Linear Unbiased Prediction procedures (BLUPs) is discussed and using a database of sensory attributes on 250 peanut cultivars and breeding lines, the application of BLUP procedures to the selection of parents for improvement of roasted peanut and sweet attributes in breeding of peanut cultivars is illustrated.}, journal={Crop biotechnology  (ACS symposium series ; 829)}, publisher={Washington, DC: American Chemical Society}, author={Pattee, H. E. and Isleib, T. G. and Giesbrecht, F. G. and Cui, Z.}, editor={K. Rajasekaran, T. J. Jacks and Finley, J. W.Editors}, year={2002}, pages={217–230} }
 @article{stalker_beute_shew_isleib_2002, title={Registration of five leaf spot-resistant peanut germplasm lines}, volume={42}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2002.0314}, abstractNote={Crop ScienceVolume 42, Issue 1 p. 314-316 Registration of Germplasm Registration of Five Leaf Spot-Resistant Peanut Germplasm Lines H.T. Stalker, Corresponding Author H.T. Stalker hts@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (hts@unity.ncsu.edu)Search for more papers by this authorM.K. Beute, M.K. Beute Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorB.B. Shew, B.B. Shew Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorT.G. Isleib, T.G. Isleib Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author H.T. Stalker, Corresponding Author H.T. Stalker hts@unity.ncsu.edu Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Corresponding author (hts@unity.ncsu.edu)Search for more papers by this authorM.K. Beute, M.K. Beute Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorB.B. Shew, B.B. Shew Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorT.G. Isleib, T.G. Isleib Dep. of Crop Science, North Carolina State Univ., Raleigh, NC, 27695-7629Search for more papers by this author First published: 01 January 2002 https://doi.org/10.2135/cropsci2002.3140Citations: 27 Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume42, Issue1January–February 2002Pages 314-316 RelatedInformation}, number={1}, journal={CROP SCIENCE}, author={Stalker, HT and Beute, MK and Shew, BB and Isleib, TG}, year={2002}, pages={314–316} }
 @article{pattee_isleib_gorbet_giesbrecht_2002, title={Selecton of alternative genetic sources of large-seed size in Virginia-type peanut: Evaluation of sensory, composition, and agronomic characteristics}, volume={50}, ISSN={["0021-8561"]}, DOI={10.1021/jf025601j}, abstractNote={Jenkins Jumbo, the ancestral source of large-seed size in the Virginia market type (Arachis hypogaea L.), has been shown to have a deleterious effect on flavor of peanut. The pervasiveness of Jenkins Jumbo in the ancestry of large-seeded germplasm contributes to the generally less intense roasted peanut flavor of U.S. cultivars of the Virginia market type. As a remedy to this problem, alternative sources of large-seed size were sought. Nine large-seeded selections, with NC 7 and Florunner as checks, were tested in replicated trials in North Carolina and Florida from 1996 to 1998. Pod yield, grade, weight of 100 seeds, and oil, sugar, and starch contents were measured. A descriptive sensory panel evaluated flavor attributes of a roasted sound mature kernel (SMK) sample from each plot. NC 7 scored low for sweet sensory attribute, high for bitter, and median for roasted peanut. UF714021, a multiline incorporating the Altika cultivar with several sister lines, had the best flavor profile of the large-seeded selections, but it did not have particularly large seeds relative to NC 7. The largest seeded selections were X90037 and X90053, both derived from Japan Jumbo. Flavor scores for X90037 were similar to those for NC 7 for roasted peanut (3.0 vs 2.9 flavor intensity units, fiu) and sweet (2.7 vs 2.6 fiu) but worse than NC 7 for bitter (3.3 vs 3.7 fiu) and astringent (3.5 vs 3.7 fiu). X90053 had intermediate values for roasted peanut and astringent, high value for sweet, and low for bitter. Other lines that had or were likely to have Jenkins Jumbo as a recent ancestor were generally poor in roasted flavor, supporting the hypothesis that ancestry from Jenkins Jumbo imparts poor flavor characteristics. With the exception of the unexpected relationship between astringent attribute and extra large kernel (ELK) content (r = 0.82, P < 0.01), there were no significant correlations between sensory attributes and the important agronomic traits: yield, meat, and ELK content. Among the nine large-seeded lines tested in this study, three appear to have greater potential for use as parents: 86x45B-10-1-2-2-b2-B, UF714021, and X90053.}, number={17}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Pattee, HE and Isleib, TG and Gorbet, DW and Giesbrecht, FG}, year={2002}, month={Aug}, pages={4885–4889} }
 @inbook{pattee_sanders_isleib_giesbrecht_2001, title={Peanut roast color and sensory attribute relationships}, DOI={10.1021/bk-2001-0775.ch013}, abstractNote={Peanut roasting develops not only a pleasing sensory flavor but also a pleasing color. In studying the genetic relationships between sensory attributes and peanut genotypes, roast color of the peanut paste test sample is an important source of variability that must be considered. Intensity of the roasted peanut sensory attribute has a quadratic relationship to CIELAB L* with an optimum for roast color at 58.7. Changes in roasted peanut, sweet, bitter, and astringent sensory attributes as roasting progresses are discussed as are the effects of peanut market-type on the intensity and rate of change in the sensory attributes. Differences in the roasted peanut quality of the peanut market-types point to the importance of cooperative efforts between plant breeders and food scientists to ensure that when new varieties are released they not only have superior agronomic characteristics but also maintain or improve upon the flavor quality characteristics.}, booktitle={Chemistry and physiology of selected food colorants}, publisher={Washington, DC: American Chemical Society}, author={Pattee, H. E. and Sanders, T. H. and Isleib, T. G. and Giesbrecht, F. G.}, editor={J. M. Ames and Hofmann, T. F.Editors}, year={2001}, pages={187–200} }
 @article{anis-ur-rehman_isleib_2001, title={Reproductive allocation on branches of Virginia-type peanut cultivars bred for yield in North Carolina}, volume={41}, number={1}, journal={Crop Science}, author={Anis-Ur-Rehman, W. R. and Isleib, T. G.}, year={2001}, pages={72–77} }
 @article{wilcut_askew_bailey_spears_isleib_2001, title={Virginia market-type peanut (Arachis hypogaea) cultivar tolerance and yield response to flumioxazin preemergence}, volume={15}, ISSN={["1550-2740"]}, DOI={10.1614/0890-037X(2001)015[0137:VMTPAH]2.0.CO;2}, abstractNote={Abstract: Field studies were conducted in 1996 and 1997 to evaluate response of eight peanut cultivars to flumioxazin applied preemergence (PRE) at 71 g ai/ha. Peanut cultivars evaluated include ‘NC 12C’, ‘NC 7’, ‘VAC 92R’, ‘NC-V 11’, ‘NC 10C’, ‘AT VC 1’, ‘NC 9’, and the experimental breeding line ‘N9001OE’. Visible injury 3 wk after planting in 1996 was 3% or less regardless of cultivar. In 1997, all cultivars were injured 15 to 28% with flumioxazin PRE, except VC 1, which was injured 45%. No visible injury was observed at 5 and 9 wk after planting. Flumioxazin did not influence the incidence of early leaf spot, late leaf spot, southern stem rot, cylindrocladium black rot, or tomato spotted wilt virus. Flumioxazin did not affect percentage of extra-large kernels, sound mature kernels, other kernels, and total yield. Nomenclature: Flumioxazin; peanut, Arachis hypogaea L., ‘NC 12C’, ‘NC 7’, ‘VAC 92R’, ‘NC-V 11’, ‘NC 10C’, ‘AT VC 1’, ‘NC 9’, ‘N9001OE’. Additional index words: Disease interaction, Cylindrocladium crotalariae (Loos) Bell and Sobers, Cercospora arachidicola Hori, Cercosporidium personatum (Berk. and Curt.), Sclerotium rolfsii Sacc., grade parameters, extra-large kernels, sound mature kernels, sound splits, total kernels, other kernels, fancy pods. Abbreviations: CBR, cylindrocladium black rot; DAP, days after planting; ELK, extra-large kernels; PPI, preplant incorporated; PRE, preemergence; SMK, sound mature kernels; SS, sound splits; TMSK, total sound mature kernels; TSWV, tomato spotted wilt virus; WAP, weeks after planting.}, number={1}, journal={WEED TECHNOLOGY}, author={Wilcut, JW and Askew, SD and Bailey, WA and Spears, JF and Isleib, TG}, year={2001}, pages={137–140} }
 @article{bailey_wilcut_spears_isleib_langston_2000, title={Diclosulam does not influence yields in eight virginia market-type peanut (Arachis hypogaea) cultivars}, volume={14}, ISSN={["1550-2740"]}, DOI={10.1614/0890-037x(2000)014[0402:ddniyi]2.0.co;2}, abstractNote={Abstract: Field studies were conducted in 1996 and 1997 to evaluate response of eight peanut cultivars to diclosulam applied preplant incorporated at 36 g ai/ha in a weed-free environment. Peanut cultivars evaluated included ‘NC 12C’, ‘NC 7’, ‘VAC 92R’, ‘NC-V 11’, ‘NC 10C’, ‘AT VC 1’,‘NC 9’, and the experimental breeding line N90010E. Visible injury 3 wk after planting was less than 5% regardless of cultivar. No injury was observed at 21 d after planting. Diclosulam did not influence the incidence of early leaf spot, late leaf spot, southern stem rot, cylindrocladium black rot, or tomato spotted wilt virus. Diclosulam did not affect percentage of extra large kernels, sound mature kernels, other kernels, and yield. Nomenclature: Diclosulam; peanut, Arachis hypogaea L. NC 12C, NC 7, VAC 92R, NC-V 11, NC 10C, AT VC 1, NC 9, N90010E. Additional index words: Disease interaction, cylindrocladium black rot, Cylindrocladium crotalariae (Loos) Bell et Sobers, early leaf spot, Cercospora arachidicola Hori, late leaf spot, Cercosporidium personatum (Berk. et Curt.), southern stem rot, Sclerotium rolfsii Sacc., tomato spotted wilt virus, grade parameters, extra large kernels, sound mature kernels, sound splits, total kernels, other kernels, fancy pods. Abbreviations: CBR, cylindrocladium black rot; DAP, days after planting; ELK, extra large kernels; PPI, preplant incorporated; SMK, sound mature kernels; SS, sound splits; TSMK, total sound mature kernels; TSWV, tomato spotted wilt virus; WAP, weeks after planting.}, number={2}, journal={WEED TECHNOLOGY}, author={Bailey, WA and Wilcut, JW and Spears, JF and Isleib, TG and Langston, VB}, year={2000}, pages={402–405} }
 @article{pattee_isleib_giesbrecht_mcfeeters_2000, title={Investigations into genotypic variations of peanut carbohydrates}, volume={48}, ISSN={["0021-8561"]}, DOI={10.1021/jf9910739}, abstractNote={Carbohydrates are known to be important precursors in the development of roasted peanut quality. However, little is known about their genotypic variation. A better understanding of the role of carbohydrates in roasted peanut quality requires first an understanding of the genotypic variation in the soluble carbohydrate components. Ion exchange chromatography was used to isolate 20 different carbohydrate components in 52 genotypes grown in replicated trials at two locations. Inositol, glucose, fructose, sucrose, raffinose, and stachyose were quantitated, and 12 unknown peaks were evaluated on the basis of the peak height of the unknown relative to the cellobiose internal standard peak height. Peaks tentatively identified as verbascose and ajugose could not be properly integrated because of tailing. Of the 18 carbohydrates that were estimated, 9 exhibited significant variation between test environments, 5 among market types, 14 among genotypes within market types, and 11 exhibited some significant form of genotype x environment interaction. Genotypes accounted for 38-78% of the total variation for the known components, suggesting that broad-sense heritability for these components is high. The observed high genotypic variation in carbohydrate components is similar to the high genotypic variation observed for the sweetness attribute in roasted peanuts, which raises the question regarding possible interrelationships. The establishment of such interrelationships could be most beneficial to peanut breeding programs to ensure the maintenance of flavor quality in future peanut varieties.}, number={3}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Pattee, HE and Isleib, TG and Giesbrecht, FG and McFeeters, RF}, year={2000}, month={Mar}, pages={750–756} }
 @article{mozingo_coffelt_isleib_2000, title={Registration of 'VA 98R' peanut}, volume={40}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2000.0051rcv}, abstractNote={Crop ScienceVolume 40, Issue 4 p. 1202-1203 Registration of Cultivar Registration of ‘VA 98R’ Peanut R.W. Mozingo, Corresponding Author R.W. Mozingo [email protected] Virginia Polytechnic Inst. and State Univ., Crop and Soil Environmental Sciences, Tidewater Agric. Res. and Ext. Center, 6321 Holland Rd., Suffolk, VA, 23437Corresponding author ([email protected]).Search for more papers by this authorT.A. Coffelt, T.A. Coffelt USDA-ARS, Phoenix, AZ, 85040Search for more papers by this authorT.G. Isleib, T.G. Isleib North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this author R.W. Mozingo, Corresponding Author R.W. Mozingo [email protected] Virginia Polytechnic Inst. and State Univ., Crop and Soil Environmental Sciences, Tidewater Agric. Res. and Ext. Center, 6321 Holland Rd., Suffolk, VA, 23437Corresponding author ([email protected]).Search for more papers by this authorT.A. Coffelt, T.A. Coffelt USDA-ARS, Phoenix, AZ, 85040Search for more papers by this authorT.G. Isleib, T.G. Isleib North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this author First published: 01 July 2000 https://doi.org/10.2135/cropsci2000.0051rcvCitations: 26 Registration by CSSA. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume40, Issue4July–August 2000Pages 1202-1203 RelatedInformation}, number={4}, journal={CROP SCIENCE}, author={Mozingo, RW and Coffelt, TA and Isleib, TG}, year={2000}, pages={1202–1203} }
 @article{pattee_isleib_giesbrecht_mcfeeters_2000, title={Relationships of sweet, bitter, and roasted peanut sensory attributes with carbohydrate components in peanuts}, volume={48}, ISSN={["0021-8561"]}, DOI={10.1021/jf9910741}, abstractNote={Certain roasted peanut quality sensory attributes have been shown to be heritable. Currently the only means of measuring these traits is the use of a trained sensory panel. This is a costly and time-consuming process. It is desirable, from a cost, time, and sample size perspective, to find other methodologies for estimating these traits. Because sweetness is the most heritable trait and it has a significant positive relationship to the roasted peanut trait, the possible relationships between heritable sensory traits and 18 carbohydrate components (inositol, glucose, fructose, sucrose, raffinose, stachyose, and 12 unknown peaks) in raw peanuts from 52 genotypes have been investigated. Previously reported correlations among sweet, bitter, and roasted peanut attributes were evident in this study as well. Where there was positive correlation of total sugars with sweetness, there also was positive correlation of total sugars with roasted peanut attribute and negative correlation of total sugars with bitterness and astringency. The expected generalized relationship of total sugars or sucrose to sweetness could not be established because the relationship was not the same across all market-types. Further work is needed to determine the nature of the chemical components related to the bitter principle, which appear to modify the sweet response and interfere with the sensory perception of sweetness, particularly in the Virginia market-type. Also, certain carbohydrate components showed significant relationships with sensory attributes in one market-type and not another. These differential associations demonstrate the complexity of the interrelationships among sweet, bitter, and roasted peanut sensory attributes. Within two market-types it is possible to improve the efficiency of selection for sweetness and roasted peanut quality by assaying for total carbohydrates. On the basis of the regression values the greatest efficiency would occur in the fastigiate market-type and then the runner.}, number={3}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Pattee, HE and Isleib, TG and Giesbrecht, FG and McFeeters, RF}, year={2000}, month={Mar}, pages={757–763} }
 @article{isleib_1999, title={Recovery of superior homozygous progeny from biparental crosses and backcrosses}, volume={39}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci1999.0011183X003900020042x}, abstractNote={Breeders of self‐pollinated species are often confronted with the question of how best to incorporate exotic germplasm into breeding populations. The objective of this work was to illustrate those situations in which a breeder might utilize one or more backcrosses to the better parent of a self‐pollinating population to increase the probability of recovery of desirable homozygous progeny from the population. The stochastic theory is presented along with probabilities associated with different conditions. The number of cycles of backcrossing needed to recover desirable progeny is a function of three variables specific to a particular pair of parents: the number of allelic differences between parents (N), the proportion (k) of desirable alleles in the better parent, and the number of loci homozygous for desirable alleles by which a progeny must exceed the number in the better parent to be selected (nd). In crosses between parents bearing equal or nearly equal numbers of desirable alleles (k close to 0.5), selfing provides the greatest probability of recovering the desired plants. As k approaches 1, the increased probability of recovering desirable alleles by backcrossing to the better parent outweighs the decreased probability of recovering desirable alleles from the worse parent, and backcrossing is indicated. With increasing N, or increasing nd, more cycles of backcrossing are needed to maximize the probability of recovery of desirable plants.}, number={2}, journal={CROP SCIENCE}, author={Isleib, TG}, year={1999}, pages={558–563} }
 @article{isleib_rice_mozingo_mozingo_pattee_1999, title={Registration of 'Gregory' peanut}, volume={39}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1999.0001rcv}, abstractNote={Crop ScienceVolume 39, Issue 5 p. 1526-1526 Registration of Cultivar Registration of ‘Gregory’ Peanut T. G. Isleib, Corresponding Author T. G. Isleib [email protected] Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Corresponding author ([email protected]).Search for more papers by this authorP. W. Rice, P. W. Rice Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorR. W. Mozingo, R. W. Mozingo Dep. of Soil and Environ. Sciences, Tidewater Agric. Res. Ext. Ctr., 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorR. W. Mozingo II, R. W. Mozingo II Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorH. E. Pattee, H. E. Pattee USDA-ARS, Box 7625, N.C State Univ., Raleigh, NC, 27695-7625Search for more papers by this author T. G. Isleib, Corresponding Author T. G. Isleib [email protected] Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Corresponding author ([email protected]).Search for more papers by this authorP. W. Rice, P. W. Rice Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorR. W. Mozingo, R. W. Mozingo Dep. of Soil and Environ. Sciences, Tidewater Agric. Res. Ext. Ctr., 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorR. W. Mozingo II, R. W. Mozingo II Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Search for more papers by this authorH. E. Pattee, H. E. Pattee USDA-ARS, Box 7625, N.C State Univ., Raleigh, NC, 27695-7625Search for more papers by this author First published: 01 September 1999 https://doi.org/10.2135/cropsci1999.0001rcvCitations: 41 Support for the development of Gregory was provided in part by the N.C Peanut Growers’ Association, Inc., the N.C. Crop Improvement Association, and the N.C. Foundation Seed Producers, Inc. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume39, Issue5September–October 1999Pages 1526-1526 RelatedInformation}, number={5}, journal={CROP SCIENCE}, author={Isleib, TG and Rice, PW and Mozingo, RW and Mozingo, RW and Pattee, HE}, year={1999}, pages={1526–1526} }
 @article{diers_isleib_sneller_boyse_1999, title={Registration of 'Titan' soybean}, volume={39}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1999.0013rcv}, abstractNote={Titan' soybean [Glycine max (L.) Merr.] (Reg. no. CV-400, PI 608438) was developed by the Michigan Agricultural Experiment Station. It was released in February 1998 because of its high yield potential compared with other cultivars of a similar maturity and its partial resistance to sclerotinia stem rot [caused by Sclerotinia sclerotiorum (Lib.) de Bary]. Titan, originally tested as E93147, is an F4-derived line selected from the cross E86067 x 'Kenwood' (1). E86067 is an experimental line developed by Michigan State University that has the pedigree A80-145015xA79-135010.A80-145015has the pedigree ('Corsoy' x 'Wayne') x Peterson '118-11' (2,3), with Peterson 118-11 having the pedigree Corsoy x ('Hawkeye' x 'Chippewa') (4,5). A79-135010 was derived from the cross Pride 'B216' x 'Cumberland' (6). Pride B216 was developed from the cross Corsoy x Wayne. The cross between E86067 and Kenwood was made during the summer of 1989 in East Lansing, MI. The F, was grown the following summer, and the F2 and F3 plants were grown in Belize during the winter of 1990-1991. The F4 plants were grown during the summer of 1991 in East Lansing and were individually threshed to composite F4-derived lines. Titan was selected based on yield and visual ratings as an F4.5 line in 1992 at East Lansing. Further yield evaluations of Titan were conducted in Michigan from 1993 to 1997. Titan was evaluated in the Preliminary Test I of the Uniform Soybean Tests-Northern Region Test (7) in 1995 and the Uniform Test I in 1996 and 1997. Titan is an indeterminate cultivar with a late Group I maturity and is best adapted to 42° to 44° N lat. In the 1996 to 1997 Uniform Soybean Test I, the maturity of Titan was 4 d later than 'Parker' (8) and 1 d earlier than 'Marcus 95'. Marcus 95 was developed by backcrossing the Rpsl-k and Rps6 genes into 'Marcus' (9). The seed yield of Titan was similar to Marcus 95 and 5% greater than Parker. Titan has better lodging scores than either Marcus 95 or Parker. The seed quality score of Titan is similar to Parker and is better than Marcus 95. The plant height of Titan is similar to Marcus 95 and is 10 cm less than Parker. Compared with Marcus 95, the seed of Titan is 5 mg seed" larger, the protein content is 8 g kg" less, and oil content is 4 g kg" less. Compared with Parker, the seed of Titan is 3 mg seed larger, the protein content is 9 g kg" less, and oil content is similar. Titan has purple flowers, tawny pubescence, brown pods at maturity, and yellow seeds with black hila. Titan does not have a major gene conferring resistance to phytophthora rot (caused by Phytophthora sojae M.J. Kaufmann & J.W. Gerdemann) and is susceptible to brown stem rot [caused by Phialophora gregata (Allington & D.W. Chamberlain) W. Gams]. Titan has partial resistance to sclerotinia stem rot. Application for U.S. plant variety protection will be made for Titan, which will permit only Foundation and Certified classes beyond breeder seed. A small sample of seed for research purposes can be obtained from the corresponding author for at least five years.}, number={5}, journal={CROP SCIENCE}, author={Diers, BW and Isleib, TG and Sneller, CH and Boyse, JF}, year={1999}, pages={1534–1534} }
 @article{pattee_giesbrecht_isleib_1999, title={Sensory attribute variation in low-temperature-stored roasted peanut paste}, volume={47}, ISSN={["1520-5118"]}, DOI={10.1021/jf981063s}, abstractNote={Length of sample storage can become significant in sensory studies due to panel fatigue limitations and samples needed for a reasonable expectation of finding significant differences. In roasted peanut sensory studies samples are stored between -10 and -23 degrees C to prevent or retard changes. Studies of up to 13 months' duration have examined stability and slow-rate sensory changes. Sweet taste was relatively stable, whereas bitter and tongue burn attributes increased slightly. Stale taste increased, suggesting lipid oxidation was taking place even at -23 degrees C. Painty attribute did not increase until stale was >3. An increase in fruity attribute was unexpected. With increases in fruity and stale attributes a decrease in roasted peanut was expected. However, storage at -23 degrees C seems to stabilize the roasted peanut lability when compared to storage at -10 degrees C. Fruity and stale interactions with roasted peanut and lability of roasted peanut were shown to be three separate and identifiable effects on roasted peanut.}, number={6}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Pattee, HE and Giesbrecht, FG and Isleib, TG}, year={1999}, month={Jun}, pages={2415–2420} }
 @article{diers_isleib_sneller_1998, title={Registration of 'Apollo' soybean}, volume={38}, DOI={10.2135/cropsci1998.0011183x003800050050x}, abstractNote={Crop ScienceVolume 38, Issue 5 cropsci1998.0011183X003800050050x p. 1400-1401 Registration of Cultivars Registration of ‘Apollo’ Soybean B. W. Diers, B. W. DiersSearch for more papers by this authorT. G. Isleib, T. G. IsleibSearch for more papers by this authorC. H. Sneller, C. H. SnellerSearch for more papers by this author B. W. Diers, B. W. DiersSearch for more papers by this authorT. G. Isleib, T. G. IsleibSearch for more papers by this authorC. H. Sneller, C. H. SnellerSearch for more papers by this author First published: 01 September 1998 https://doi.org/10.2135/cropsci1998.0011183X003800050050xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume38, Issue5September–October 1998Pages 1400-1401 RelatedInformation}, number={5}, journal={Crop Science}, author={Diers, B. W. and Isleib, T. G. and Sneller, C. H.}, year={1998}, pages={1400–1401} }
 @article{diers_isleib_sneller_1998, title={Registration of 'Olympus' soybean}, volume={38}, DOI={10.2135/cropsci1998.0011183x003800050049x}, abstractNote={Crop ScienceVolume 38, Issue 5 cropsci1998.0011183X003800050049x p. 1400-1400 Registration of Cultivars Registration of ‘Olympus’ Soybean B. W. Diers, B. W. DiersSearch for more papers by this authorT. G. Isleib, T. G. IsleibSearch for more papers by this authorC. H. Sneller, C. H. SnellerSearch for more papers by this author B. W. Diers, B. W. DiersSearch for more papers by this authorT. G. Isleib, T. G. IsleibSearch for more papers by this authorC. H. Sneller, C. H. SnellerSearch for more papers by this author First published: 01 September 1998 https://doi.org/10.2135/cropsci1998.0011183X003800050049xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume38, Issue5September–October 1998Pages 1400-1400 RelatedInformation}, number={5}, journal={Crop Science}, author={Diers, B. W. and Isleib, T. G. and Sneller, C. H.}, year={1998}, pages={1400} }
 @article{isleib_1997, title={Cost-effective transfer of recessive traits via the backcross procedure}, volume={37}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1997.0011183X003700010024x}, abstractNote={The backcross breeding method is often used to transfer recessive traits controlled by one or a few genes from one pure line to another. Guidelines are needed to help plant breeders to use backcrossing cost‐effectively. In a backcrossing program, a plant breeder may choose between methods with two or three stages per cycle. The two‐stage method utilizes alternate backcrossing and selfing to identify BCiSj plants with the recessive trait while the three‐stage method uses two sequential crosses followed by selfing. In the three‐stage method, nmin, the minimum number of plants required to recover at least one or two BCiSj plants in the ith cycle with a given probability (1 — α), is obtained by growing only one BCiSj plant for each of nmin BCiSo. Alternative values for the number of BCiSo plants (rib) and BCiSj plants per BCiSo (ns are present for α = 0.05 and α = 0.01 for traits controlled by one, two, three, or four recessive genetic loci. A method to compare the cost‐effectiveness of alternative values is presented for cases where the relative costs of crossing, selfing, and evaluation of Sj progeny are known. Unless time is the paramount concern, it is only in cases where the ressive trait is controlled by a single locus that it can be more cost‐effective to make two sequential crosses to the recurrent parent before selflng than to cross and self and then only if the cost of evaluating BCiSj plants is high relative to the cost of producing BCiSo plants. When using the three‐stage backcross method, the breeder can reduce the nb and increase ns if the cost of evaluating BCiSj plants is low relative to the cost of producing BCiSo plants.}, number={1}, journal={CROP SCIENCE}, author={Isleib, TG}, year={1997}, pages={139–144} }
 @article{isleib_rice_bailey_mozingo_pattee_1997, title={Registration of 'NC 12C' peanut}, volume={37}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1997.0011183X003700060051x}, abstractNote={Crop ScienceVolume 37, Issue 6 cropsci1997.0011183X003700060051x p. 1976-1976 Registration of Cultivars Registration of ‘NC 12C’ Peanut T. G. Isleib, Corresponding Author T. G. Isleib [email protected] Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Corresponding author ([email protected]).Search for more papers by this authorP. W. Rice, P. W. Rice Dep. of Plant Pathology, N.C. State Univ., Box 7616, Raleigh, NC, 27695-7616Search for more papers by this authorJ. E. Bailey, J. E. Bailey Dep. of Soil and Environ. Sciences Tidewater Agric. Res. Ext. Ctr., 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorR. W. Mozingo, R. W. Mozingo USDA-ARS, Box 7625, N.C. State Univ., Raleigh, NC, 27695-7625Search for more papers by this authorH. E. Pattee, H. E. Pattee USDA-ARS, Box 7625, N.C. State Univ., Raleigh, NC, 27695-7625Search for more papers by this author T. G. Isleib, Corresponding Author T. G. Isleib [email protected] Dep. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC, 27695-7629Corresponding author ([email protected]).Search for more papers by this authorP. W. Rice, P. W. Rice Dep. of Plant Pathology, N.C. State Univ., Box 7616, Raleigh, NC, 27695-7616Search for more papers by this authorJ. E. Bailey, J. E. Bailey Dep. of Soil and Environ. Sciences Tidewater Agric. Res. Ext. Ctr., 6321 Holland Rd., Suffolk, VA, 23437Search for more papers by this authorR. W. Mozingo, R. W. Mozingo USDA-ARS, Box 7625, N.C. State Univ., Raleigh, NC, 27695-7625Search for more papers by this authorH. E. Pattee, H. E. Pattee USDA-ARS, Box 7625, N.C. State Univ., Raleigh, NC, 27695-7625Search for more papers by this author First published: 01 November 1997 https://doi.org/10.2135/cropsci1997.0011183X003700060051xCitations: 21AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume37, Issue6November–December 1997Pages 1976-1976 RelatedInformation}, number={6}, journal={CROP SCIENCE}, author={Isleib, TG and Rice, PW and Bailey, JE and Mozingo, RW and Pattee, HE}, year={1997}, pages={1976–1976} }
 @article{isleib_young_knauft_1996, title={Fatty acid genotypes of five Virginia-type peanut cultivars}, volume={36}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1996.0011183X003600030003x}, abstractNote={Reduction of linoleic acid in the fatty acid profile of Virginia‐type peanut (Arachis hypogaea L.) cultivars is desirable because it would improve the stability and shelf‐life of in‐shell and other peanut products. A low‐linoleate/high‐oleate trait controlled by two recessive genes, ol1 and ol2, has been identified in Univ. of Florida breeding line F435. Most runner‐type cultivars and breeding lines genotyped to date were found to differ from F435 by only one ol allele. Genotypes differing from F435 at two ol loci were Virginia types. The number of genes controlling inheritance of the trait has major implications with respect to the numbers of backcross and F2 test progeny required in an efficient backcross breeding program. To determine the number of genes differentiating F435 from large‐seeded Virginia‐type cultivars, F435 was crossed as a male with five common Virginia‐type cultivars. F4:5 progenies were assayed for fatty acid content by gas chromatography. Progenies were classified as having high or low oleate levels and chi square analysis was applied to the data. Segregation ratios of populations derived from crosses with ‘NC 7’, ‘NC 9’, ‘NC 10C’, and ‘VA‐C 92R’ were consistent with a monogenic model and inconsistent with the digenic model. These cultivars have genotype Ol1Ol1ol2ol2 or ol1ol1Ol2Ol2. Progeny of ‘NC‐V 11’ / F435 followed a ratio consistent with the digenic model and inconsistent with the monogenic model, indicating that NC‐V 11 has genotype Ol1Ol1Ol2Ol2. Oleate levels were elevated by 220 to 280 g kg−1, linoleate levels decreased by 190 to 270 g kg−1, and palmitate levels decreased by 30 to 40 g kg−1 in high‐oleate subpopulations. Other fatty acids exhibited changes that were statistically significant but probably too small in magnitude to significantly alter processing quality or stability of oil.}, number={3}, journal={CROP SCIENCE}, author={Isleib, TG and Young, CT and Knauft, DA}, year={1996}, pages={556–558} }
 @article{isleib_sneller_diers_1994, title={Registration of 'Dimon' soybean}, volume={34}, DOI={10.2135/cropsci1994.0011183x003400010077x}, abstractNote={Crop ScienceVolume 34, Issue 1 cropsci1994.0011183X003400010077x p. 311-311 Registration of Cultivars Registration of ‘Dimon’ Soybean T. G. Isleib, Corresponding Author T. G. Isleib n/a@.dne Dep. of Crop Sci., North Carolina State Univ., Raleigh, NC, 27695-7620Corresponding author.Search for more papers by this authorC. H. Sneller, C. H. Sneller Dep. of Agronomy, Univ. of Arkansas, Fayetteville, AR, 72701Search for more papers by this authorB W. Diers, B W. Diers Dep. of Crop and Soil Sci., Michigan State Univ., East Lansing, MI, 48824Search for more papers by this author T. G. Isleib, Corresponding Author T. G. Isleib n/a@.dne Dep. of Crop Sci., North Carolina State Univ., Raleigh, NC, 27695-7620Corresponding author.Search for more papers by this authorC. H. Sneller, C. H. Sneller Dep. of Agronomy, Univ. of Arkansas, Fayetteville, AR, 72701Search for more papers by this authorB W. Diers, B W. Diers Dep. of Crop and Soil Sci., Michigan State Univ., East Lansing, MI, 48824Search for more papers by this author First published: 01 January 1994 https://doi.org/10.2135/cropsci1994.0011183X003400010077xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume34, Issue1January–February 1994Pages 311-311 RelatedInformation}, number={1}, journal={Crop Science}, author={Isleib, T. G. and Sneller, C. H. and Diers, B. W.}, year={1994}, pages={311} }
 @article{isleib_sneller_diers_1994, title={Registration of 'Felix' soybean}, volume={34}, DOI={10.2135/cropsci1994.0011183x003400010078x}, abstractNote={Crop ScienceVolume 34, Issue 1 cropsci1994.0011183X003400010078x p. 311-312 Registration of Cultivars Registration of ‘Felix’ Soybean T. G. Isleib, Corresponding Author T. G. Isleib n/a@.dne Dep. of Crop Sci., N.C. State Univ., Raleigh, NC, 27695-7620Corresponding author.Search for more papers by this authorC. H. Sneller, C. H. Sneller Dep. of Agronomy, Univ. of Ark., Fayetteville, AR, 72701Search for more papers by this authorB W. Diers, B W. Diers Dep. of Crop and Soil Sci., Mich. State Univ., East Lansing, MI, 48824Search for more papers by this author T. G. Isleib, Corresponding Author T. G. Isleib n/a@.dne Dep. of Crop Sci., N.C. State Univ., Raleigh, NC, 27695-7620Corresponding author.Search for more papers by this authorC. H. Sneller, C. H. Sneller Dep. of Agronomy, Univ. of Ark., Fayetteville, AR, 72701Search for more papers by this authorB W. Diers, B W. Diers Dep. of Crop and Soil Sci., Mich. State Univ., East Lansing, MI, 48824Search for more papers by this author First published: 01 January 1994 https://doi.org/10.2135/cropsci1994.0011183X003400010078xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume34, Issue1January–February 1994Pages 311-312 RelatedInformation}, number={1}, journal={Crop Science}, author={Isleib, T. G. and Sneller, C. H. and Diers, B. W.}, year={1994}, pages={311} }